CNN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CNN1
Entrez Gene ID	850607
Full Name	centromere-binding protein CNN1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180011.1	NP_116704.1	centromere-binding protein CNN1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Phosphorylation of the Cnn1 N-terminus coordinates the binding of the two competing Ndc80 interaction partners.
Title: A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors.

Cnn1 regulates KMN activity in a spatiotemporal manner by inhibiting the interaction between its complexes
Title: Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore.

Data show that cells that fail to activate Cdk1 are devoid of spindles due to persistently active APCCdh1, which targets microtubule-associated proteins Cin8, Kip1 and Ase1 for degradation.
Title: Cdk1 regulates centrosome separation by restraining proteolysis of microtubule-associated proteins.

The following CNN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CNN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00284	NM_001180011.1 Latest version!	Saccharomyces cerevisiae S288C centromere-binding protein CNN1 (CNN1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CNN1 ( NM_001180011.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116704.1 Saccharomyces cerevisiae S288C centromere-binding protein CNN1 (CNN1), partial mRNA.

Price & Availability↑

CloneID	OSi00284
Clone ID Related Accession (Same CDS sequence)	NM_001180011.1
Accession Version	NM_001180011.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1086bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	centromere-binding protein CNN1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAGCACTC CCAGGAAGGC CGCCGGTAAC AACGAGAATA CAGAAGTCTC AGAGATCAGA 
ACCCCCTTTC GGGAGAGAGC CCTTGAAGAA CAACGTCTCA AGGATGAGGT ACTGATCAGA 
AATACGCCAG GTTATAGAAA ACTTTTATCT GCTTCTACTA AGTCTCATGA CATCCTCAAT 
AAAGATCCGA ACGAAGTACG AAGTTTTTTA CAAGACTTAA GCCAAGTGTT AGCACGCAAA 
TCACAAGGCA ACGATACAAC TACTAATAAA ACTCAAGCAC GGAATCTAAT AGATGAATTA 
GCGTACGAAG AGAGCCAACC AGAGGAGAAT GAGCTTCTTC GGAGCAGAAG TGAGAAACTG 
ACAGATAACA ACATTGGCAA TGAAACGCAA CCTGATTATA CTTCCTTATC GCAAACAGTA 
TTTGCAAAAT TGCAGGAAAG AGACAAAGGC TTGAAAAGTA GGAAGATTGA CCCGATAATA 
ATACAAGATG TTCCCACGAC AGGACACGAG GATGAATTGA CAGTGCACTC TCCAGATAAA 
GCTAACAGTA TCTCTATGGA AGTTTTGCGC ACGTCGCCAA GCATTGGAAT GGATCAGGTA 
GATGAACCGC CAGTACGTGA TCCGGTACCA ATTTCCATAA CGCAACAGGA AGAACCACTA 
TCTGAAGATT TACCAAGCGA CGATAAAGAG GAGACAGAAG AAGCAGAAAA CGAAGATTAT 
TCGTTTGAAA ACACCAGTGA CGAAAATTTG GATGATATAG GCAACGATCC TATCCGTTTA 
AATGTCCCTG CGGTGAGACG TTCTTCTATA AAACCGCTGC AAATAATGGA TCTAAAACAT 
CTTACAAGGC AATTTTTGAA TGAGAATAGG ATAATATTGC CGAAACAAAC GTGGTCTACT 
ATACAGGAGG AATCTTTAAA TATAATGGAT TTCTTGAAAC AAAAAATCGG CACGTTACAA 
AAACAGGAAT TGGTAGATTC GTTTATCGAT ATGGGTATTA TCAATAACGT TGATGATATG 
TTTGAGCTCG CGCACGAATT GCTTCCCTTA GAACTTCAAT CAAGAATTGA AAGTTATTTG 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116704.1
CDS	1..1086
Translation	MSTPRKAAGNNENTEVSEIRTPFRERALEEQRLKDEVLIRNTPGYRKLLSASTKSHDILNKDPNEVRSFLQDLSQVLARKSQGNDTTTNKTQARNLIDELAYEESQPEENELLRSRSEKLTDNNIGNETQPDYTSLSQTVFAKLQERDKGLKSRKIDPIIIQDVPTTGHEDELTVHSPDKANSISMEVLRTSPSIGMDQVDEPPVRDPVPISITQQEEPLSEDLPSDDKEETEEAENEDYSFENTSDENLDDIGNDPIRLNVPAVRRSSIKPLQIMDLKHLTRQFLNENRIILPKQTWSTIQEESLNIMDFLKQKIGTLQKQELVDSFIDMGIINNVDDMFELAHELLPLELQSRIESYLF

Target ORF information:

RefSeq Version	NM_001180011.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C centromere-binding protein CNN1 (CNN1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180011.1

ORF Insert Sequence:

ATGAGCACTC CCAGGAAGGC CGCCGGTAAC AACGAGAATA CAGAAGTCTC AGAGATCAGA 
ACCCCCTTTC GGGAGAGAGC CCTTGAAGAA CAACGTCTCA AGGATGAGGT ACTGATCAGA 
AATACGCCAG GTTATAGAAA ACTTTTATCT GCTTCTACTA AGTCTCATGA CATCCTCAAT 
AAAGATCCGA ACGAAGTACG AAGTTTTTTA CAAGACTTAA GCCAAGTGTT AGCACGCAAA 
TCACAAGGCA ACGATACAAC TACTAATAAA ACTCAAGCAC GGAATCTAAT AGATGAATTA 
GCGTACGAAG AGAGCCAACC AGAGGAGAAT GAGCTTCTTC GGAGCAGAAG TGAGAAACTG 
ACAGATAACA ACATTGGCAA TGAAACGCAA CCTGATTATA CTTCCTTATC GCAAACAGTA 
TTTGCAAAAT TGCAGGAAAG AGACAAAGGC TTGAAAAGTA GGAAGATTGA CCCGATAATA 
ATACAAGATG TTCCCACGAC AGGACACGAG GATGAATTGA CAGTGCACTC TCCAGATAAA 
GCTAACAGTA TCTCTATGGA AGTTTTGCGC ACGTCGCCAA GCATTGGAAT GGATCAGGTA 
GATGAACCGC CAGTACGTGA TCCGGTACCA ATTTCCATAA CGCAACAGGA AGAACCACTA 
TCTGAAGATT TACCAAGCGA CGATAAAGAG GAGACAGAAG AAGCAGAAAA CGAAGATTAT 
TCGTTTGAAA ACACCAGTGA CGAAAATTTG GATGATATAG GCAACGATCC TATCCGTTTA 
AATGTCCCTG CGGTGAGACG TTCTTCTATA AAACCGCTGC AAATAATGGA TCTAAAACAT 
CTTACAAGGC AATTTTTGAA TGAGAATAGG ATAATATTGC CGAAACAAAC GTGGTCTACT 
ATACAGGAGG AATCTTTAAA TATAATGGAT TTCTTGAAAC AAAAAATCGG CACGTTACAA 
AAACAGGAAT TGGTAGATTC GTTTATCGAT ATGGGTATTA TCAATAACGT TGATGATATG 
TTTGAGCTCG CGCACGAATT GCTTCCCTTA GAACTTCAAT CAAGAATTGA AAGTTATTTG 
TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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CNN1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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