BST1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BST1
Entrez Gene ID	850519
Full Name	Bst1p
Synonyms	PER17
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179941.1	NP_116628.1	Bst1p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F25124g	XP_456200.1
Neurospora crassa	NCU08021	XP_962691.1
Saccharomyces cerevisiae (baker's yeast)	BST1	NP_116628.1
Schizosaccharomyces pombe (fission yeast)	SPAC824.02	NP_593441.1
Magnaporthe oryzae (rice blast fungus)	MGG_01844	XP_003714864.1
Eremothecium gossypii	AGOS_AFR521W	NP_986068.2

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Glycosylphosphatidylinositol (GPI) inositol deacylation by Bst1p plays an important role in the quality control and ER-associated degradation of glycosylphosphatidylinositol-anchored proteins.
Title: Inositol deacylation by Bst1p is required for the quality control of glycosylphosphatidylinositol-anchored proteins.

The following BST1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BST1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00211	NM_001179941.1 Latest version!	Saccharomyces cerevisiae S288C Bst1p (BST1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BST1 ( NM_001179941.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116628.1 Saccharomyces cerevisiae S288C Bst1p (BST1), partial mRNA.

Price & Availability↑

CloneID	OSi00211
Clone ID Related Accession (Same CDS sequence)	NM_001179941.1
Accession Version	NM_001179941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Bst1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGTATCA GGAGATTAGT TAGTGTTATA ACGCGGCCCA TAATTAATAA AGTTAACTCA 
TCTGGTCAAT ACTCGAGAGT ACTCGCTACG CGTGAAGACC AGGATAAAGC TTCCCCCAAA 
TATATGAACA ATGATAAGAT AGCAAAAAAA CCTTACACAT ATCGCCTTTT CTCTATTCTT 
GGCATTTTAA GCATTTGCTC GCTTTTATTG ATATCGCTAC TGAAACCTTT TAATGGTGCA 
GATGCTCCTC AGTGTGAATC TATCTATATG TTTCCATCTT ACGCAAGGAT CGATGGATTT 
GACGAGAGGT ATACACCACT GGCACACAAG TATCATCTTT ACTTGTATCG AGAACAAAGC 
GTAGACCGAG AACCTTTAAA TGGTGATGAG TTACAACTTG ATGGTATCCC TGTACTTTTT 
ATACCCGGAA ATGCAGGTTC TTTTAGGCAA TGCAGATCGA TTGCTTCCGC TTGCTCCAAT 
ATATATTTTG ATTCCAATAC CAGGGCGACT CTGCGTAACG AAAATGTGCG AAATTTGGAC 
TTCTTTACGG CAGATTTCAA TGAAGATTTC ACTGCGTTTC ATGGTGAAAC AATGCTTGAT 
CAAGCTGAAT ACCTAAATGA TGCCATTAAG TACATCTTGT CTCTTTACGA AAGGACCCCT 
GATTACCCAC ATCCAAAACC GCAGTCAGTT ATTATCGTGG GACATTCCAT GGGCGGTATT 
GTATCCAGGG TCATGCTAAC TTTGAAGAAC CACGTACCGG GAAGTATAAG CACCATATTG 
ACCTTATCAT CACCTCATGC TGCTTCACCA GTAACCTTTG ATGGTGACAT TTTAAAGCTT 
TACAAAAATA CAAACGAATA TTGGAGAAAG CAGTTATCCC AAAACGATTC TTTTTTCTCT 
AAGAACATTT CATTGGTTTC CATTACAGGC GGTATATTGG ATACTACTTT ACCTGCAGAT 
TATGCATCTG TAGAAGATTT GGTTAGCTTA GAAAATGGGT TCACCAGCTT CACAACTACT 
ATTCCAGATG TTTGGACTCC AATTGATCAT TTAGCCATTG TTTGGTGCAA GCAATTAAGA 
GAGGTACTAG CGCGCCTTTT ATTGGAAAGT ATAGATGCCT CCAAACCTGA AAAAGTTAAG 
CCATTGAATC AGAGATTGCA AATCGCTAGA AAACTATTGC TTTCAGGGTT TGAAGACTAC 
TCATGGATGA ATAGTAAGTT AAATTATCCT CAAGAAAACT TACAAGAATT TAGTGACAAC 
TTCTTTTCTG ATTACGCTAC TTTGGAAATG AACGATGTCC TAGATTTTGA AATGTTTAAC 
CTGGAAAAGT GGCACAACAA TTATACAAAA ATAAATATTC CGAGTAACAT CTCCTCCACT 
GAACACCTGC ATTTCACATT ATTAACTAGT CTCGATATGC CTATGATCTA TTTCTGTACA 
GAGTCCAGGG TGAATCTAAG CTGTATAACA GCTGTTGATA GTATTCTAAC AGTGCCCAGG 
TCTTCAAAAG ATACTCAGTT CGCAGCTGAT TCTTCTTTTG GAGAAGCAAA AAATCCGTTT 
AAAGCCGTTA GTGTTGGCAA GAATATACTA CAGAAGTACG ACTACTTGAT GATAAGCAAA 
CCCACTTACG GTGAATTTTC AGAACAAGAA GGCATGGAGG ACAATCAGGG GTTTCTTTTG 
GCATTACTGC GCAACGTCTC CAATGTACAA ATAGTCAATA CTACACCTTC TCAAATATTG 
CTCTTTGGAG AACAACTACA CTTGGATGGC AAAGACATCG AGCAGGTTAT AAGTTTCAGT 
AATTTGTGGG ATTCTTTATT ATCGTACAAA TTAGAAACCA AGATTGAGGC AAGCAACGAA 
GGTATTGCTT CCGAAGAGAC ATTGTTTCAG CCTTTCATAA GGCAATGGGT TTACGAACCT 
TTCGAGTCTA AATGGCATCT AAATATTATA AACAAAAGTC TCGATATAAA TATGCATAAT 
GTAGCACCGT TTATCCCATT AAATGAAAGC GAACCGAGAT CGCTACAATT AAGTTTTTTT 
ATTCCACCCG GAATGTCCTT AGAAGCTAAA ATGACTATTA ATTGGTCTTT AACTTTGAAA 
ATGCTGTTTA TCAGGTACAG ATTAGCACTA GCATCTTTTC CAGTCGCGTT TATAGCACTC 
GTACTGTCAT ACCAGTTCTA CTGGTACAAC AAGACATCTG AGTTTCCTTC CTTTGATAGC 
ACTTTGGGAT ATATTTTAAG AAAGCACGGA ATATTAATGT TTTTTACGCT GTTCTTAGCT 
TCGCCTGTGG TTAATAATAA GTTGGTACAA CGAATACTTT ATTTATTGGA TCCTGTCGGT 
TTAAATTATC CATTTCTGCT ATCCGAGAGA AACATGCATG CAAATTTTTA TTATTTGGGG 
ATCAGAGATT GGTTTATGTC AACTATTGGA ATTCTCTTTG GGGTTATGAC AGTTGGATTA 
CTAGCACTAG TTTCTAAGAT TTTTGGATCC TTGGAAATTT TAGTAATATT CCTTCAACGG 
AAGTTATCAA AGAAAAACAC TGAAGATAAA GAGGCTTTTG ATACTATTGA ACATAAAGCG 
TATGGTAAGG GAAGATTAAT GGCATCTGTG CTTTTATTAC TGTTGGTATT TTTTCACATA 
CCATACCAGA TGGCGTTTGT TATCTCATTA GTAATCCAAA TTGCTACCTG CATTAGAGTA 
GCGCTGTTGA AACTATCAAA CAACGAGCAG AAATTGAACT TACTAAATTA TAATATGACG 
TTGCTTCTGC TTTTATTATT TGTTAGTGCC ATTAACATCC CTATCATTAT TGTTTTCCTG 
CATAATGTTG CCATCAAATG GGAAACTTCA TTTAGATCCC ATCACAATAT ACTAGCAGTG 
GCACCAATTA TTTTTTTGGT TGGAAATAAT TCTATTTTCA AAATGCCAAA CAGCGTACCT 
TTAGACACGT GGGATGGTAA AGTTACCATC ATTTTATTTG TGTACTTAAC CGTTTTCAGT 
TTCATTTATG GAATAAGAAA TCTGTACTGG ATACACCACT TGGTGAACAT AATATGTGCC 
TGGTTGCTAT TTTTCGAAAC AATACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116628.1
CDS	1..3090
Translation	MGIRRLVSVITRPIINKVNSSGQYSRVLATREDQDKASPKYMNNDKIAKKPYTYRLFSILGILSICSLLLISLLKPFNGADAPQCESIYMFPSYARIDGFDERYTPLAHKYHLYLYREQSVDREPLNGDELQLDGIPVLFIPGNAGSFRQCRSIASACSNIYFDSNTRATLRNENVRNLDFFTADFNEDFTAFHGETMLDQAEYLNDAIKYILSLYERTPDYPHPKPQSVIIVGHSMGGIVSRVMLTLKNHVPGSISTILTLSSPHAASPVTFDGDILKLYKNTNEYWRKQLSQNDSFFSKNISLVSITGGILDTTLPADYASVEDLVSLENGFTSFTTTIPDVWTPIDHLAIVWCKQLREVLARLLLESIDASKPEKVKPLNQRLQIARKLLLSGFEDYSWMNSKLNYPQENLQEFSDNFFSDYATLEMNDVLDFEMFNLEKWHNNYTKINIPSNISSTEHLHFTLLTSLDMPMIYFCTESRVNLSCITAVDSILTVPRSSKDTQFAADSSFGEAKNPFKAVSVGKNILQKYDYLMISKPTYGEFSEQEGMEDNQGFLLALLRNVSNVQIVNTTPSQILLFGEQLHLDGKDIEQVISFSNLWDSLLSYKLETKIEASNEGIASEETLFQPFIRQWVYEPFESKWHLNIINKSLDINMHNVAPFIPLNESEPRSLQLSFFIPPGMSLEAKMTINWSLTLKMLFIRYRLALASFPVAFIALVLSYQFYWYNKTSEFPSFDSTLGYILRKHGILMFFTLFLASPVVNNKLVQRILYLLDPVGLNYPFLLSERNMHANFYYLGIRDWFMSTIGILFGVMTVGLLALVSKIFGSLEILVIFLQRKLSKKNTEDKEAFDTIEHKAYGKGRLMASVLLLLLVFFHIPYQMAFVISLVIQIATCIRVALLKLSNNEQKLNLLNYNMTLLLLLLFVSAINIPIIIVFLHNVAIKWETSFRSHHNILAVAPIIFLVGNNSIFKMPNSVPLDTWDGKVTIILFVYLTVFSFIYGIRNLYWIHHLVNIICAWLLFFETIH

Target ORF information:

RefSeq Version	NM_001179941.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Bst1p (BST1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179941.1

ORF Insert Sequence:

ATGGGTATCA GGAGATTAGT TAGTGTTATA ACGCGGCCCA TAATTAATAA AGTTAACTCA 
TCTGGTCAAT ACTCGAGAGT ACTCGCTACG CGTGAAGACC AGGATAAAGC TTCCCCCAAA 
TATATGAACA ATGATAAGAT AGCAAAAAAA CCTTACACAT ATCGCCTTTT CTCTATTCTT 
GGCATTTTAA GCATTTGCTC GCTTTTATTG ATATCGCTAC TGAAACCTTT TAATGGTGCA 
GATGCTCCTC AGTGTGAATC TATCTATATG TTTCCATCTT ACGCAAGGAT CGATGGATTT 
GACGAGAGGT ATACACCACT GGCACACAAG TATCATCTTT ACTTGTATCG AGAACAAAGC 
GTAGACCGAG AACCTTTAAA TGGTGATGAG TTACAACTTG ATGGTATCCC TGTACTTTTT 
ATACCCGGAA ATGCAGGTTC TTTTAGGCAA TGCAGATCGA TTGCTTCCGC TTGCTCCAAT 
ATATATTTTG ATTCCAATAC CAGGGCGACT CTGCGTAACG AAAATGTGCG AAATTTGGAC 
TTCTTTACGG CAGATTTCAA TGAAGATTTC ACTGCGTTTC ATGGTGAAAC AATGCTTGAT 
CAAGCTGAAT ACCTAAATGA TGCCATTAAG TACATCTTGT CTCTTTACGA AAGGACCCCT 
GATTACCCAC ATCCAAAACC GCAGTCAGTT ATTATCGTGG GACATTCCAT GGGCGGTATT 
GTATCCAGGG TCATGCTAAC TTTGAAGAAC CACGTACCGG GAAGTATAAG CACCATATTG 
ACCTTATCAT CACCTCATGC TGCTTCACCA GTAACCTTTG ATGGTGACAT TTTAAAGCTT 
TACAAAAATA CAAACGAATA TTGGAGAAAG CAGTTATCCC AAAACGATTC TTTTTTCTCT 
AAGAACATTT CATTGGTTTC CATTACAGGC GGTATATTGG ATACTACTTT ACCTGCAGAT 
TATGCATCTG TAGAAGATTT GGTTAGCTTA GAAAATGGGT TCACCAGCTT CACAACTACT 
ATTCCAGATG TTTGGACTCC AATTGATCAT TTAGCCATTG TTTGGTGCAA GCAATTAAGA 
GAGGTACTAG CGCGCCTTTT ATTGGAAAGT ATAGATGCCT CCAAACCTGA AAAAGTTAAG 
CCATTGAATC AGAGATTGCA AATCGCTAGA AAACTATTGC TTTCAGGGTT TGAAGACTAC 
TCATGGATGA ATAGTAAGTT AAATTATCCT CAAGAAAACT TACAAGAATT TAGTGACAAC 
TTCTTTTCTG ATTACGCTAC TTTGGAAATG AACGATGTCC TAGATTTTGA AATGTTTAAC 
CTGGAAAAGT GGCACAACAA TTATACAAAA ATAAATATTC CGAGTAACAT CTCCTCCACT 
GAACACCTGC ATTTCACATT ATTAACTAGT CTCGATATGC CTATGATCTA TTTCTGTACA 
GAGTCCAGGG TGAATCTAAG CTGTATAACA GCTGTTGATA GTATTCTAAC AGTGCCCAGG 
TCTTCAAAAG ATACTCAGTT CGCAGCTGAT TCTTCTTTTG GAGAAGCAAA AAATCCGTTT 
AAAGCCGTTA GTGTTGGCAA GAATATACTA CAGAAGTACG ACTACTTGAT GATAAGCAAA 
CCCACTTACG GTGAATTTTC AGAACAAGAA GGCATGGAGG ACAATCAGGG GTTTCTTTTG 
GCATTACTGC GCAACGTCTC CAATGTACAA ATAGTCAATA CTACACCTTC TCAAATATTG 
CTCTTTGGAG AACAACTACA CTTGGATGGC AAAGACATCG AGCAGGTTAT AAGTTTCAGT 
AATTTGTGGG ATTCTTTATT ATCGTACAAA TTAGAAACCA AGATTGAGGC AAGCAACGAA 
GGTATTGCTT CCGAAGAGAC ATTGTTTCAG CCTTTCATAA GGCAATGGGT TTACGAACCT 
TTCGAGTCTA AATGGCATCT AAATATTATA AACAAAAGTC TCGATATAAA TATGCATAAT 
GTAGCACCGT TTATCCCATT AAATGAAAGC GAACCGAGAT CGCTACAATT AAGTTTTTTT 
ATTCCACCCG GAATGTCCTT AGAAGCTAAA ATGACTATTA ATTGGTCTTT AACTTTGAAA 
ATGCTGTTTA TCAGGTACAG ATTAGCACTA GCATCTTTTC CAGTCGCGTT TATAGCACTC 
GTACTGTCAT ACCAGTTCTA CTGGTACAAC AAGACATCTG AGTTTCCTTC CTTTGATAGC 
ACTTTGGGAT ATATTTTAAG AAAGCACGGA ATATTAATGT TTTTTACGCT GTTCTTAGCT 
TCGCCTGTGG TTAATAATAA GTTGGTACAA CGAATACTTT ATTTATTGGA TCCTGTCGGT 
TTAAATTATC CATTTCTGCT ATCCGAGAGA AACATGCATG CAAATTTTTA TTATTTGGGG 
ATCAGAGATT GGTTTATGTC AACTATTGGA ATTCTCTTTG GGGTTATGAC AGTTGGATTA 
CTAGCACTAG TTTCTAAGAT TTTTGGATCC TTGGAAATTT TAGTAATATT CCTTCAACGG 
AAGTTATCAA AGAAAAACAC TGAAGATAAA GAGGCTTTTG ATACTATTGA ACATAAAGCG 
TATGGTAAGG GAAGATTAAT GGCATCTGTG CTTTTATTAC TGTTGGTATT TTTTCACATA 
CCATACCAGA TGGCGTTTGT TATCTCATTA GTAATCCAAA TTGCTACCTG CATTAGAGTA 
GCGCTGTTGA AACTATCAAA CAACGAGCAG AAATTGAACT TACTAAATTA TAATATGACG 
TTGCTTCTGC TTTTATTATT TGTTAGTGCC ATTAACATCC CTATCATTAT TGTTTTCCTG 
CATAATGTTG CCATCAAATG GGAAACTTCA TTTAGATCCC ATCACAATAT ACTAGCAGTG 
GCACCAATTA TTTTTTTGGT TGGAAATAAT TCTATTTTCA AAATGCCAAA CAGCGTACCT 
TTAGACACGT GGGATGGTAA AGTTACCATC ATTTTATTTG TGTACTTAAC CGTTTTCAGT 
TTCATTTATG GAATAAGAAA TCTGTACTGG ATACACCACT TGGTGAACAT AATATGTGCC 
TGGTTGCTAT TTTTCGAAAC AATACATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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BST1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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