STE2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	STE2
Entrez Gene ID	850518
Full Name	alpha-factor pheromone receptor STE2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179940.2	NP_116627.2	alpha-factor pheromone receptor STE2

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	STE2	NP_116627.2
Kluyveromyces lactis	KLLA0F25102g	XP_456199.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

yeast pheromone receptor Ste2 forms predominantly tetramers at average expression levels of 2 to 25 molecules per pixel and a mixture of tetramers and octamers at expression levels of 25-100 molecules per pixel. Ste2 is a class D GPCR found in the yeast Saccharomyces cerevisiae of the mating type a, and binds the pheromone a-factor secreted secreted by cells of the mating type a
Title: Quaternary structure of the yeast pheromone receptor Ste2 in living cells.

Results suggest that the G-protein-coupled receptor, Ste2 binds the inhibitory G-protein signaling, Sst2 forming a complex with antagonistic functions.
Title: Yeast GPCR signaling reflects the fraction of occupied receptors, not the number.

Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast
Title: Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast.

The main difference between the Ste2 and Ste3 receptor observed in this work was that while hyperactive mutations were found for Ste3, no gain-of-function mutant for the Ste2 receptor was found. this fi nding is an outcome of the different strategies the two genes have adopted for their expression.
Title: Analysis of random PCR-originated mutants of the yeast Ste2 and Ste3 receptors.

we propose that the N-terminus of Ste2p plays multiple regulatory roles in controlling receptor function.
Title: The N-terminus of the yeast G protein-coupled receptor Ste2p plays critical roles in surface expression, signaling, and negative regulation.

The following STE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00210	NM_001179940.2 Latest version!	Saccharomyces cerevisiae S288C alpha-factor pheromone receptor STE2 (STE2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STE2 ( NM_001179940.2 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116627.2 Saccharomyces cerevisiae S288C alpha-factor pheromone receptor STE2 (STE2), partial mRNA.

Price & Availability↑

CloneID	OSi00210
Clone ID Related Accession (Same CDS sequence)	NM_001179940.2
Accession Version	NM_001179940.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	alpha-factor pheromone receptor STE2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). On Apr 26, 2011 this sequence version replaced NM_001179940.1. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGATG CGGCTCCTTC ATTGAGCAAT CTATTTTATG ATCCAACGTA TAATCCTGGT 
CAAAGCACCA TTAACTACAC TTCCATATAT GGGAATGGAT CTACCATCAC TTTCGATGAG 
TTGCAAGGTT TAGTTAACAG TACTGTTACT CAGGCCATTA TGTTTGGTGT CAGATGTGGT 
GCAGCTGCTT TGACTTTGAT TGTCATGTGG ATGACATCGA GAAGCAGAAA AACGCCGATT 
TTCATTATCA ACCAAGTTTC ATTGTTTTTA ATCATTTTGC ATTCTGCACT CTATTTTAAA 
TATTTACTGT CTAATTACTC TTCAGTGACT TACGCTCTCA CCGGATTTCC TCAGTTCATC 
AGTAGAGGTG ACGTTCATGT TTATGGTGCT ACAAATATAA TTCAAGTCCT TCTTGTGGCT 
TCTATTGAGA CTTCACTGGT GTTTCAGATA AAAGTTATTT TCACAGGCGA CAACTTCAAA 
AGGATAGGTT TGATGCTGAC GTCGATATCT TTCACTTTAG GGATTGCTAC AGTTACCATG 
TATTTTGTAA GCGCTGTTAA AGGTATGATT GTGACTTATA ATGATGTTAG TGCCACCCAA 
GATAAATACT TCAATGCATC CACAATTTTA CTTGCATCCT CAATAAACTT TATGTCATTT 
GTCCTGGTAG TTAAATTGAT TTTAGCTATT AGATCAAGAA GATTCCTTGG TCTCAAGCAG 
TTCGATAGTT TCCATATTTT ACTCATAATG TCATGTCAAT CTTTGTTGGT TCCATCGATA 
ATATTCATCC TCGCATACAG TTTGAAACCA AACCAGGGAA CAGATGTCTT GACTACTGTT 
GCAACATTAC TTGCTGTATT GTCTTTACCA TTATCATCAA TGTGGGCCAC GGCTGCTAAT 
AATGCATCCA AAACAAACAC AATTACTTCA GACTTTACAA CATCCACAGA TAGGTTTTAT 
CCAGGCACGC TGTCTAGCTT TCAAACTGAT AGTATCAACA ACGATGCTAA AAGCAGTCTC 
AGAAGTAGAT TATATGACCT ATATCCTAGA AGGAAGGAAA CAACATCGGA TAAACATTCG 
GAAAGAACTT TTGTTTCTGA GACTGCAGAT GATATAGAGA AAAATCAGTT TTATCAGTTG 
CCCACACCTA CGAGTTCAAA AAATACTAGG ATAGGACCGT TTGCTGATGC AAGTTACAAA 
GAGGGAGAAG TTGAACCCGT CGACATGTAC ACTCCCGATA CGGCAGCTGA TGAGGAAGCC 
AGAAAGTTCT GGACTGAAGA TAATAATAAT TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116627.2
CDS	1..1296
Translation	MSDAAPSLSNLFYDPTYNPGQSTINYTSIYGNGSTITFDELQGLVNSTVTQAIMFGVRCGAAALTLIVMWMTSRSRKTPIFIINQVSLFLIILHSALYFKYLLSNYSSVTYALTGFPQFISRGDVHVYGATNIIQVLLVASIETSLVFQIKVIFTGDNFKRIGLMLTSISFTLGIATVTMYFVSAVKGMIVTYNDVSATQDKYFNASTILLASSINFMSFVLVVKLILAIRSRRFLGLKQFDSFHILLIMSCQSLLVPSIIFILAYSLKPNQGTDVLTTVATLLAVLSLPLSSMWATAANNASKTNTITSDFTTSTDRFYPGTLSSFQTDSINNDAKSSLRSRLYDLYPRRKETTSDKHSERTFVSETADDIEKNQFYQLPTPTSSKNTRIGPFADASYKEGEVEPVDMYTPDTAADEEARKFWTEDNNNL

Target ORF information:

RefSeq Version	NM_001179940.2
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C alpha-factor pheromone receptor STE2 (STE2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179940.2

ORF Insert Sequence:

ATGTCTGATG CGGCTCCTTC ATTGAGCAAT CTATTTTATG ATCCAACGTA TAATCCTGGT 
CAAAGCACCA TTAACTACAC TTCCATATAT GGGAATGGAT CTACCATCAC TTTCGATGAG 
TTGCAAGGTT TAGTTAACAG TACTGTTACT CAGGCCATTA TGTTTGGTGT CAGATGTGGT 
GCAGCTGCTT TGACTTTGAT TGTCATGTGG ATGACATCGA GAAGCAGAAA AACGCCGATT 
TTCATTATCA ACCAAGTTTC ATTGTTTTTA ATCATTTTGC ATTCTGCACT CTATTTTAAA 
TATTTACTGT CTAATTACTC TTCAGTGACT TACGCTCTCA CCGGATTTCC TCAGTTCATC 
AGTAGAGGTG ACGTTCATGT TTATGGTGCT ACAAATATAA TTCAAGTCCT TCTTGTGGCT 
TCTATTGAGA CTTCACTGGT GTTTCAGATA AAAGTTATTT TCACAGGCGA CAACTTCAAA 
AGGATAGGTT TGATGCTGAC GTCGATATCT TTCACTTTAG GGATTGCTAC AGTTACCATG 
TATTTTGTAA GCGCTGTTAA AGGTATGATT GTGACTTATA ATGATGTTAG TGCCACCCAA 
GATAAATACT TCAATGCATC CACAATTTTA CTTGCATCCT CAATAAACTT TATGTCATTT 
GTCCTGGTAG TTAAATTGAT TTTAGCTATT AGATCAAGAA GATTCCTTGG TCTCAAGCAG 
TTCGATAGTT TCCATATTTT ACTCATAATG TCATGTCAAT CTTTGTTGGT TCCATCGATA 
ATATTCATCC TCGCATACAG TTTGAAACCA AACCAGGGAA CAGATGTCTT GACTACTGTT 
GCAACATTAC TTGCTGTATT GTCTTTACCA TTATCATCAA TGTGGGCCAC GGCTGCTAAT 
AATGCATCCA AAACAAACAC AATTACTTCA GACTTTACAA CATCCACAGA TAGGTTTTAT 
CCAGGCACGC TGTCTAGCTT TCAAACTGAT AGTATCAACA ACGATGCTAA AAGCAGTCTC 
AGAAGTAGAT TATATGACCT ATATCCTAGA AGGAAGGAAA CAACATCGGA TAAACATTCG 
GAAAGAACTT TTGTTTCTGA GACTGCAGAT GATATAGAGA AAAATCAGTT TTATCAGTTG 
CCCACACCTA CGAGTTCAAA AAATACTAGG ATAGGACCGT TTGCTGATGC AAGTTACAAA 
GAGGGAGAAG TTGAACCCGT CGACATGTAC ACTCCCGATA CGGCAGCTGA TGAGGAAGCC 
AGAAAGTTCT GGACTGAAGA TAATAATAAT TTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

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STE2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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