MAN2B1 cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAN2B1
Entrez Gene ID	107519445
Full Name	mannosidase alpha class 2B member 1
Gene Type	protein-coding
Organism	Rousettus aegyptiacus(Egyptian rousette)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016162161.1	XP_016017647.1	lysosomal alpha-mannosidase isoform X1
XM_016162162.1	XP_016017648.1	lysosomal alpha-mannosidase isoform X2

Pathways from BioSystems
Gene Ontology

The following MAN2B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORo23788	XM_016162161.1 Latest version!	Rousettus aegyptiacus mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
ORo23789	XM_016162162.1 Latest version!	Rousettus aegyptiacus mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAN2B1 ( XM_016162161.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_016017647.1 Rousettus aegyptiacus mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORo23788
Clone ID Related Accession (Same CDS sequence)	XM_016162161.1
Accession Version	XM_016162161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	lysosomal alpha-mannosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015494751.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCG ACACGCCGCC TTCGGGGGTC CGCGCTGGCC GCGGCCAGGG CGCGGCGGGG 
CCCTGGACAA GCTCCAGCGC ACTGCTGCCA CTGCTCCCGC CTCTCTTCTT CCTTTTCTTG 
CTGCTGGTCG CGCCCGGCGC TCAGGCCGCG GGTTACGAGA CATGCCCCAC GGTGCAGCCG 
GACATGATCA ACGTGCACCT GGTGGCACAC ACACATGATG ACGTGGGCTG GCTCAAGACG 
GTGGACCAGT ACTTTTATGG CATTGACAAT GATGTCCAGC ATGCAGGTGT GCAGTACATC 
CTAGACTCCG TCATCTCTTC CCTGCTGGCA GAGCCCACTC GCCGCTTCAT CTATGTGGAG 
ATGGCCTTCT TCTCCCGTTG GTGGCACCAA CAGACAAATG CAACACAGGA AGTTGTGCGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GCCAACGGTG GCTGGGTTAT GAACGATGAG 
GCAGCCACCC ACTATGGAGC AATCATAGAC CAGATGACAC TCGGACTGCG CTTCCTGGAA 
GATACGTTTG GCAACAACGG GCGCCCCCGT GTGGCCTGGC ACATTGACCC CTTCGGGCAT 
TCTCGGGAGC AGGCCTCCCT GTTCGCGCAG ATGGGCTTTG ACGGCTTCTT CTTCGGGCGC 
CTGGATTATC AAGATAAGTG GGTTCGGAAA AAGCAGCTAA GGATGGAGCA GGTGTGGCGA 
GCAAGTGCCA GCCTGAAGCC CCCCGCTGCC GACCTCTTCA CCAGTGTGCT CCCCAACATG 
TACAACCCGC CGATGAACCT GTGCTGGGAT GTGATGTGCG TTGACAAACC TATCGTGGAA 
GACCCCCGCA GCCCCGAGTA CAATGCCAAG GAACTGGTTG ATTACTTCCT GAAATTAGCC 
ACAGTCCAGA GTGGGTACTA CCGCACCAAC CACACCATAA TGACCATGGG CTCAGACTTC 
CAGTATGAGA ATGCCAACAT GTGGTTTAAG AACCTTGATA AGCTCATCCA TCTGGTCAAT 
GCTCAGCAGC AGGCCAATGG GAGCCACGTC AATGTTCTCT ACTCCACTCC GGCCTGTTAC 
CTCTGGGAGC TGAACAAGGC CAACCTCACC TGGTCGGCGA AACAGGATGA CTTCTTCCCC 
TACGCCGATG GCCCCCACCA GTTCTGGACA GGCTTCTTTT CCAGCCGGCC GGGCCTCAAA 
CGCTACGAGC GTCTCAGCTA TAACTTCCTG CAGGTGTGCA ACCAGCTGGA GGCGCTGGCA 
GGTCCGGCGG CCAACGTGGG ACCTTATGGC TCGGGAGACA GCGCACCCCT CAATGAGGCG 
ATGGCTGTGC TCCAGCACCA TGATGCGGTC AGTGGTACCT CCCGGCAGCA CGTGGCAGAC 
GACTATGCTC GCCAGCTTGC CGCAGGCTGG GGACCCTGTG AGGTTCTCCT GAGTAACGCG 
CTGGCACGGC TCAGCGGCTC CAAGGAGGAA TTTGCGTTCT GCCGCTGGCT CAACATCAGC 
GTCTGTCCGC TTAGCCAGAC CGCGGAGATC TTCCAGGTGA CTATTTATAA CCCCTTGGGG 
CGGAAAGTGG AATGGATGGT GCGGCTTCCG GTCAGCAAAC ACATATTCCT CGTGAAAGAT 
CCCAATGGCA CAGTTATACC CAGCGATGTG GTTACAATGC CCAGCTCAGA CAGACAGGAG 
CTGCTTTTCT CAGCCTCAGT GCCTGCCCTC GGCTTCAGCA TCTACTCAGT AACCCAGGTG 
CCTGGCCAGA GCCCACAGGC TCACACCTCC AAGCCCAGAT CCCAGAAGTC CTCGTCCCGC 
ATCTTGACCA TCCAAAATGA GTACATCCGG GCTAGATTTG ATCCTGACAC AGGGCTCTTG 
GTGGAGTTGG AGAACATGGA CCAGAAACTC CTGCTGCCTG TTCGCCAAGC CTTCTACTGG 
TACAACGCCA GTACAGGCAA CTACCTAAGT GACCAGGTCT CAGGTGCCTA CATCTTCAGA 
CCCAACCGAC AGGAACCACT GCTCGTGAGC CGCTGGGCTC AAATCCACCT GGTGAAGAAG 
GCCTTGGTGC AGGAGGTACA CCAGAACTTC TCAGCCTGGT GTTCCCAAGT GGTTCGTCTG 
TATCCAGGAC AGCGGCACCT GGAGCTGGAG TGGACAGTGG GACCAATACC TGTGGGTGAT 
GGCTGGGGAA AGGAGGTCAT TAGCCGTTTT GACACTGAGC TGGAGACAAA AGGACTCTTC 
TTCACTGACA GCAATGGCCG GGAGATCCTG GAGAGAAGAC GGAATTATCG ACCCACATGG 
GTGCTGAACA TGACTGAGCC AGTGGCAGGA AACTACTATC CAGTCAACAG TCGCATTTAC 
ATTACGGACG GGAAAGCGCA ACTGACGGTG CTGACAGACC GTTCCCAGGG CGGCAGCAGC 
CTGAAAGATG GCTCCGTAGA GCTCATGGTG CACCGAAGGC TGCTGAAAGA CGATAGGCGC 
GGAGTTGGGG AGCCACTGCT GGAGAATGGG TCGGGGTCGG GGTTGTGGGT GCGGGGGCGC 
CACCTCGTGC TGCTGGATAA GGCCCGGACC GCCGCTGTCA GGCACCGGTT GCAGGCGGAG 
AAGGAGCTCC TGGCCCCGCA GGTGGTGCTG GCCCAGGGTA GCGGCACTCC CTACCACCCG 
GGGGTCACCC CGCGCAGGCA GTTCTCCGGG CTGCGCAGGG AGCTGCCGCC CAACGTGCAC 
CTGCTTACCC TCGCCCGCTG GGGCCGGGAA ACGCTGCTGC TGCGCTTAGA GCATCAGTTC 
GCCGTACAGG AGGATATGGT CGGCAACTTG AGCTCTCCGG TGACCTTGGA CTTGAGGAAC 
CTGTTTTCTG CCTTCACCAT CACTGACCTT AAGGAAACCA CGCTTGCTGC CAACCAGCTA 
CGGGCCAGTG CCTCCAGGCT CCAGTGGACA GTGAACACGG ATGAGGGCCA CACACCCAAG 
TCCTCTCCCT TCCAACTGGA CCCTGCCAGT GTCACACTGC AGCCCATGGA AATCCGCACC 
TTCCTGGCCT CAGTCCAGTG GGAAAAGGAC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016017647.1
CDS	29..3064
Translation	MDADTPPSGVRAGRGQGAAGPWTSSSALLPLLPPLFFLFLLLVAPGAQAAGYETCPTVQPDMINVHLVAHTHDDVGWLKTVDQYFYGIDNDVQHAGVQYILDSVISSLLAEPTRRFIYVEMAFFSRWWHQQTNATQEVVRNLVRQGRLEFANGGWVMNDEAATHYGAIIDQMTLGLRFLEDTFGNNGRPRVAWHIDPFGHSREQASLFAQMGFDGFFFGRLDYQDKWVRKKQLRMEQVWRASASLKPPAADLFTSVLPNMYNPPMNLCWDVMCVDKPIVEDPRSPEYNAKELVDYFLKLATVQSGYYRTNHTIMTMGSDFQYENANMWFKNLDKLIHLVNAQQQANGSHVNVLYSTPACYLWELNKANLTWSAKQDDFFPYADGPHQFWTGFFSSRPGLKRYERLSYNFLQVCNQLEALAGPAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVADDYARQLAAGWGPCEVLLSNALARLSGSKEEFAFCRWLNISVCPLSQTAEIFQVTIYNPLGRKVEWMVRLPVSKHIFLVKDPNGTVIPSDVVTMPSSDRQELLFSASVPALGFSIYSVTQVPGQSPQAHTSKPRSQKSSSRILTIQNEYIRARFDPDTGLLVELENMDQKLLLPVRQAFYWYNASTGNYLSDQVSGAYIFRPNRQEPLLVSRWAQIHLVKKALVQEVHQNFSAWCSQVVRLYPGQRHLELEWTVGPIPVGDGWGKEVISRFDTELETKGLFFTDSNGREILERRRNYRPTWVLNMTEPVAGNYYPVNSRIYITDGKAQLTVLTDRSQGGSSLKDGSVELMVHRRLLKDDRRGVGEPLLENGSGSGLWVRGRHLVLLDKARTAAVRHRLQAEKELLAPQVVLAQGSGTPYHPGVTPRRQFSGLRRELPPNVHLLTLARWGRETLLLRLEHQFAVQEDMVGNLSSPVTLDLRNLFSAFTITDLKETTLAANQLRASASRLQWTVNTDEGHTPKSSPFQLDPASVTLQPMEIRTFLASVQWEKDS

Target ORF information:

RefSeq Version	XM_016162161.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016162161.1

ORF Insert Sequence:

ATGGATGCCG ACACGCCGCC TTCGGGGGTC CGCGCTGGCC GCGGCCAGGG CGCGGCGGGG 
CCCTGGACAA GCTCCAGCGC ACTGCTGCCA CTGCTCCCGC CTCTCTTCTT CCTTTTCTTG 
CTGCTGGTCG CGCCCGGCGC TCAGGCCGCG GGTTACGAGA CATGCCCCAC GGTGCAGCCG 
GACATGATCA ACGTGCACCT GGTGGCACAC ACACATGATG ACGTGGGCTG GCTCAAGACG 
GTGGACCAGT ACTTTTATGG CATTGACAAT GATGTCCAGC ATGCAGGTGT GCAGTACATC 
CTAGACTCCG TCATCTCTTC CCTGCTGGCA GAGCCCACTC GCCGCTTCAT CTATGTGGAG 
ATGGCCTTCT TCTCCCGTTG GTGGCACCAA CAGACAAATG CAACACAGGA AGTTGTGCGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GCCAACGGTG GCTGGGTTAT GAACGATGAG 
GCAGCCACCC ACTATGGAGC AATCATAGAC CAGATGACAC TCGGACTGCG CTTCCTGGAA 
GATACGTTTG GCAACAACGG GCGCCCCCGT GTGGCCTGGC ACATTGACCC CTTCGGGCAT 
TCTCGGGAGC AGGCCTCCCT GTTCGCGCAG ATGGGCTTTG ACGGCTTCTT CTTCGGGCGC 
CTGGATTATC AAGATAAGTG GGTTCGGAAA AAGCAGCTAA GGATGGAGCA GGTGTGGCGA 
GCAAGTGCCA GCCTGAAGCC CCCCGCTGCC GACCTCTTCA CCAGTGTGCT CCCCAACATG 
TACAACCCGC CGATGAACCT GTGCTGGGAT GTGATGTGCG TTGACAAACC TATCGTGGAA 
GACCCCCGCA GCCCCGAGTA CAATGCCAAG GAACTGGTTG ATTACTTCCT GAAATTAGCC 
ACAGTCCAGA GTGGGTACTA CCGCACCAAC CACACCATAA TGACCATGGG CTCAGACTTC 
CAGTATGAGA ATGCCAACAT GTGGTTTAAG AACCTTGATA AGCTCATCCA TCTGGTCAAT 
GCTCAGCAGC AGGCCAATGG GAGCCACGTC AATGTTCTCT ACTCCACTCC GGCCTGTTAC 
CTCTGGGAGC TGAACAAGGC CAACCTCACC TGGTCGGCGA AACAGGATGA CTTCTTCCCC 
TACGCCGATG GCCCCCACCA GTTCTGGACA GGCTTCTTTT CCAGCCGGCC GGGCCTCAAA 
CGCTACGAGC GTCTCAGCTA TAACTTCCTG CAGGTGTGCA ACCAGCTGGA GGCGCTGGCA 
GGTCCGGCGG CCAACGTGGG ACCTTATGGC TCGGGAGACA GCGCACCCCT CAATGAGGCG 
ATGGCTGTGC TCCAGCACCA TGATGCGGTC AGTGGTACCT CCCGGCAGCA CGTGGCAGAC 
GACTATGCTC GCCAGCTTGC CGCAGGCTGG GGACCCTGTG AGGTTCTCCT GAGTAACGCG 
CTGGCACGGC TCAGCGGCTC CAAGGAGGAA TTTGCGTTCT GCCGCTGGCT CAACATCAGC 
GTCTGTCCGC TTAGCCAGAC CGCGGAGATC TTCCAGGTGA CTATTTATAA CCCCTTGGGG 
CGGAAAGTGG AATGGATGGT GCGGCTTCCG GTCAGCAAAC ACATATTCCT CGTGAAAGAT 
CCCAATGGCA CAGTTATACC CAGCGATGTG GTTACAATGC CCAGCTCAGA CAGACAGGAG 
CTGCTTTTCT CAGCCTCAGT GCCTGCCCTC GGCTTCAGCA TCTACTCAGT AACCCAGGTG 
CCTGGCCAGA GCCCACAGGC TCACACCTCC AAGCCCAGAT CCCAGAAGTC CTCGTCCCGC 
ATCTTGACCA TCCAAAATGA GTACATCCGG GCTAGATTTG ATCCTGACAC AGGGCTCTTG 
GTGGAGTTGG AGAACATGGA CCAGAAACTC CTGCTGCCTG TTCGCCAAGC CTTCTACTGG 
TACAACGCCA GTACAGGCAA CTACCTAAGT GACCAGGTCT CAGGTGCCTA CATCTTCAGA 
CCCAACCGAC AGGAACCACT GCTCGTGAGC CGCTGGGCTC AAATCCACCT GGTGAAGAAG 
GCCTTGGTGC AGGAGGTACA CCAGAACTTC TCAGCCTGGT GTTCCCAAGT GGTTCGTCTG 
TATCCAGGAC AGCGGCACCT GGAGCTGGAG TGGACAGTGG GACCAATACC TGTGGGTGAT 
GGCTGGGGAA AGGAGGTCAT TAGCCGTTTT GACACTGAGC TGGAGACAAA AGGACTCTTC 
TTCACTGACA GCAATGGCCG GGAGATCCTG GAGAGAAGAC GGAATTATCG ACCCACATGG 
GTGCTGAACA TGACTGAGCC AGTGGCAGGA AACTACTATC CAGTCAACAG TCGCATTTAC 
ATTACGGACG GGAAAGCGCA ACTGACGGTG CTGACAGACC GTTCCCAGGG CGGCAGCAGC 
CTGAAAGATG GCTCCGTAGA GCTCATGGTG CACCGAAGGC TGCTGAAAGA CGATAGGCGC 
GGAGTTGGGG AGCCACTGCT GGAGAATGGG TCGGGGTCGG GGTTGTGGGT GCGGGGGCGC 
CACCTCGTGC TGCTGGATAA GGCCCGGACC GCCGCTGTCA GGCACCGGTT GCAGGCGGAG 
AAGGAGCTCC TGGCCCCGCA GGTGGTGCTG GCCCAGGGTA GCGGCACTCC CTACCACCCG 
GGGGTCACCC CGCGCAGGCA GTTCTCCGGG CTGCGCAGGG AGCTGCCGCC CAACGTGCAC 
CTGCTTACCC TCGCCCGCTG GGGCCGGGAA ACGCTGCTGC TGCGCTTAGA GCATCAGTTC 
GCCGTACAGG AGGATATGGT CGGCAACTTG AGCTCTCCGG TGACCTTGGA CTTGAGGAAC 
CTGTTTTCTG CCTTCACCAT CACTGACCTT AAGGAAACCA CGCTTGCTGC CAACCAGCTA 
CGGGCCAGTG CCTCCAGGCT CCAGTGGACA GTGAACACGG ATGAGGGCCA CACACCCAAG 
TCCTCTCCCT TCCAACTGGA CCCTGCCAGT GTCACACTGC AGCCCATGGA AATCCGCACC 
TTCCTGGCCT CAGTCCAGTG GGAAAAGGAC AGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2B1 ( XM_016162162.1 ) cDNA ORF clone, Rousettus aegyptiacus(Egyptian rousette) -> XP_016017648.1 Rousettus aegyptiacus mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORo23789
Clone ID Related Accession (Same CDS sequence)	XM_016162162.1
Accession Version	XM_016162162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-28
Organism	Rousettus aegyptiacus(Egyptian rousette)
Product	lysosomal alpha-mannosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015494751.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rousettus aegyptiacus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCCG ACACGCCGCC TTCGGGGGTC CGCGCTGGCC GCGGCCAGGG CGCGGCGGGG 
CCCTGGACAA GCTCCAGCGC ACTGCTGCCA CTGCTCCCGC CTCTCTTCTT CCTTTTCTTG 
CTGCTGGTCG CGCCCGGCGC TCAGGCCGCG GGTTACGAGA CATGCCCCAC GGTGCAGCCG 
GACATGATCA ACGTGCACCT GGTGGCACAC ACACATGATG ACGTGGGCTG GCTCAAGACG 
GTGGACCAGT ACTTTTATGG CATTGACAAT GATGTCCAGC ATGCAGGTGT GCAGTACATC 
CTAGACTCCG TCATCTCTTC CCTGCTGGCA GAGCCCACTC GCCGCTTCAT CTATGTGGAG 
ATGGCCTTCT TCTCCCGTTG GTGGCACCAA CAGACAAATG CAACACAGGA AGTTGTGCGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GCCAACGGTG GCTGGGTTAT GAACGATGAG 
GCAGCCACCC ACTATGGAGC AATCATAGAC CAGATGACAC TCGGACTGCG CTTCCTGGAA 
GATACGTTTG GCAACAACGG GCGCCCCCGT GTGGCCTGGC ACATTGACCC CTTCGGGCAT 
TCTCGGGAGC AGGCCTCCCT GTTCGCGCAG ATGGGCTTTG ACGGCTTCTT CTTCGGGCGC 
CTGGATTATC AAGATAAGTG GGTTCGGAAA AAGCAGCTAA GGATGGAGCA GGTGTGGCGA 
GCAAGTGCCA GCCTGAAGCC CCCCGCTGCC GACCTCTTCA CCAGTGTGCT CCCCAACATG 
TACAACCCGC CGATGAACCT GTGCTGGGAT GTGATGTGCG TTGACAAACC TATCGTGGAA 
GACCCCCGCA GCCCCGAGTA CAATGCCAAG GAACTGGTTG ATTACTTCCT GAAATTAGCC 
ACAGTCCAGA GTGGGTACTA CCGCACCAAC CACACCATAA TGACCATGGG CTCAGACTTC 
CAGTATGAGA ATGCCAACAT GTGGTTTAAG AACCTTGATA AGCTCATCCA TCTGGTCAAT 
GCTCAGCAGG CCAATGGGAG CCACGTCAAT GTTCTCTACT CCACTCCGGC CTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA CCTCACCTGG TCGGCGAAAC AGGATGACTT CTTCCCCTAC 
GCCGATGGCC CCCACCAGTT CTGGACAGGC TTCTTTTCCA GCCGGCCGGG CCTCAAACGC 
TACGAGCGTC TCAGCTATAA CTTCCTGCAG GTGTGCAACC AGCTGGAGGC GCTGGCAGGT 
CCGGCGGCCA ACGTGGGACC TTATGGCTCG GGAGACAGCG CACCCCTCAA TGAGGCGATG 
GCTGTGCTCC AGCACCATGA TGCGGTCAGT GGTACCTCCC GGCAGCACGT GGCAGACGAC 
TATGCTCGCC AGCTTGCCGC AGGCTGGGGA CCCTGTGAGG TTCTCCTGAG TAACGCGCTG 
GCACGGCTCA GCGGCTCCAA GGAGGAATTT GCGTTCTGCC GCTGGCTCAA CATCAGCGTC 
TGTCCGCTTA GCCAGACCGC GGAGATCTTC CAGGTGACTA TTTATAACCC CTTGGGGCGG 
AAAGTGGAAT GGATGGTGCG GCTTCCGGTC AGCAAACACA TATTCCTCGT GAAAGATCCC 
AATGGCACAG TTATACCCAG CGATGTGGTT ACAATGCCCA GCTCAGACAG ACAGGAGCTG 
CTTTTCTCAG CCTCAGTGCC TGCCCTCGGC TTCAGCATCT ACTCAGTAAC CCAGGTGCCT 
GGCCAGAGCC CACAGGCTCA CACCTCCAAG CCCAGATCCC AGAAGTCCTC GTCCCGCATC 
TTGACCATCC AAAATGAGTA CATCCGGGCT AGATTTGATC CTGACACAGG GCTCTTGGTG 
GAGTTGGAGA ACATGGACCA GAAACTCCTG CTGCCTGTTC GCCAAGCCTT CTACTGGTAC 
AACGCCAGTA CAGGCAACTA CCTAAGTGAC CAGGTCTCAG GTGCCTACAT CTTCAGACCC 
AACCGACAGG AACCACTGCT CGTGAGCCGC TGGGCTCAAA TCCACCTGGT GAAGAAGGCC 
TTGGTGCAGG AGGTACACCA GAACTTCTCA GCCTGGTGTT CCCAAGTGGT TCGTCTGTAT 
CCAGGACAGC GGCACCTGGA GCTGGAGTGG ACAGTGGGAC CAATACCTGT GGGTGATGGC 
TGGGGAAAGG AGGTCATTAG CCGTTTTGAC ACTGAGCTGG AGACAAAAGG ACTCTTCTTC 
ACTGACAGCA ATGGCCGGGA GATCCTGGAG AGAAGACGGA ATTATCGACC CACATGGGTG 
CTGAACATGA CTGAGCCAGT GGCAGGAAAC TACTATCCAG TCAACAGTCG CATTTACATT 
ACGGACGGGA AAGCGCAACT GACGGTGCTG ACAGACCGTT CCCAGGGCGG CAGCAGCCTG 
AAAGATGGCT CCGTAGAGCT CATGGTGCAC CGAAGGCTGC TGAAAGACGA TAGGCGCGGA 
GTTGGGGAGC CACTGCTGGA GAATGGGTCG GGGTCGGGGT TGTGGGTGCG GGGGCGCCAC 
CTCGTGCTGC TGGATAAGGC CCGGACCGCC GCTGTCAGGC ACCGGTTGCA GGCGGAGAAG 
GAGCTCCTGG CCCCGCAGGT GGTGCTGGCC CAGGGTAGCG GCACTCCCTA CCACCCGGGG 
GTCACCCCGC GCAGGCAGTT CTCCGGGCTG CGCAGGGAGC TGCCGCCCAA CGTGCACCTG 
CTTACCCTCG CCCGCTGGGG CCGGGAAACG CTGCTGCTGC GCTTAGAGCA TCAGTTCGCC 
GTACAGGAGG ATATGGTCGG CAACTTGAGC TCTCCGGTGA CCTTGGACTT GAGGAACCTG 
TTTTCTGCCT TCACCATCAC TGACCTTAAG GAAACCACGC TTGCTGCCAA CCAGCTACGG 
GCCAGTGCCT CCAGGCTCCA GTGGACAGTG AACACGGATG AGGGCCACAC ACCCAAGTCC 
TCTCCCTTCC AACTGGACCC TGCCAGTGTC ACACTGCAGC CCATGGAAAT CCGCACCTTC 
CTGGCCTCAG TCCAGTGGGA AAAGGACAGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016017648.1
CDS	28..3060
Translation	MDADTPPSGVRAGRGQGAAGPWTSSSALLPLLPPLFFLFLLLVAPGAQAAGYETCPTVQPDMINVHLVAHTHDDVGWLKTVDQYFYGIDNDVQHAGVQYILDSVISSLLAEPTRRFIYVEMAFFSRWWHQQTNATQEVVRNLVRQGRLEFANGGWVMNDEAATHYGAIIDQMTLGLRFLEDTFGNNGRPRVAWHIDPFGHSREQASLFAQMGFDGFFFGRLDYQDKWVRKKQLRMEQVWRASASLKPPAADLFTSVLPNMYNPPMNLCWDVMCVDKPIVEDPRSPEYNAKELVDYFLKLATVQSGYYRTNHTIMTMGSDFQYENANMWFKNLDKLIHLVNAQQANGSHVNVLYSTPACYLWELNKANLTWSAKQDDFFPYADGPHQFWTGFFSSRPGLKRYERLSYNFLQVCNQLEALAGPAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVADDYARQLAAGWGPCEVLLSNALARLSGSKEEFAFCRWLNISVCPLSQTAEIFQVTIYNPLGRKVEWMVRLPVSKHIFLVKDPNGTVIPSDVVTMPSSDRQELLFSASVPALGFSIYSVTQVPGQSPQAHTSKPRSQKSSSRILTIQNEYIRARFDPDTGLLVELENMDQKLLLPVRQAFYWYNASTGNYLSDQVSGAYIFRPNRQEPLLVSRWAQIHLVKKALVQEVHQNFSAWCSQVVRLYPGQRHLELEWTVGPIPVGDGWGKEVISRFDTELETKGLFFTDSNGREILERRRNYRPTWVLNMTEPVAGNYYPVNSRIYITDGKAQLTVLTDRSQGGSSLKDGSVELMVHRRLLKDDRRGVGEPLLENGSGSGLWVRGRHLVLLDKARTAAVRHRLQAEKELLAPQVVLAQGSGTPYHPGVTPRRQFSGLRRELPPNVHLLTLARWGRETLLLRLEHQFAVQEDMVGNLSSPVTLDLRNLFSAFTITDLKETTLAANQLRASASRLQWTVNTDEGHTPKSSPFQLDPASVTLQPMEIRTFLASVQWEKDS

Target ORF information:

RefSeq Version	XM_016162162.1
Organism	Rousettus aegyptiacus(Egyptian rousette)
Definition	Rousettus aegyptiacus mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016162162.1

ORF Insert Sequence:

ATGGATGCCG ACACGCCGCC TTCGGGGGTC CGCGCTGGCC GCGGCCAGGG CGCGGCGGGG 
CCCTGGACAA GCTCCAGCGC ACTGCTGCCA CTGCTCCCGC CTCTCTTCTT CCTTTTCTTG 
CTGCTGGTCG CGCCCGGCGC TCAGGCCGCG GGTTACGAGA CATGCCCCAC GGTGCAGCCG 
GACATGATCA ACGTGCACCT GGTGGCACAC ACACATGATG ACGTGGGCTG GCTCAAGACG 
GTGGACCAGT ACTTTTATGG CATTGACAAT GATGTCCAGC ATGCAGGTGT GCAGTACATC 
CTAGACTCCG TCATCTCTTC CCTGCTGGCA GAGCCCACTC GCCGCTTCAT CTATGTGGAG 
ATGGCCTTCT TCTCCCGTTG GTGGCACCAA CAGACAAATG CAACACAGGA AGTTGTGCGG 
AACCTGGTGC GCCAGGGGCG CCTGGAGTTT GCCAACGGTG GCTGGGTTAT GAACGATGAG 
GCAGCCACCC ACTATGGAGC AATCATAGAC CAGATGACAC TCGGACTGCG CTTCCTGGAA 
GATACGTTTG GCAACAACGG GCGCCCCCGT GTGGCCTGGC ACATTGACCC CTTCGGGCAT 
TCTCGGGAGC AGGCCTCCCT GTTCGCGCAG ATGGGCTTTG ACGGCTTCTT CTTCGGGCGC 
CTGGATTATC AAGATAAGTG GGTTCGGAAA AAGCAGCTAA GGATGGAGCA GGTGTGGCGA 
GCAAGTGCCA GCCTGAAGCC CCCCGCTGCC GACCTCTTCA CCAGTGTGCT CCCCAACATG 
TACAACCCGC CGATGAACCT GTGCTGGGAT GTGATGTGCG TTGACAAACC TATCGTGGAA 
GACCCCCGCA GCCCCGAGTA CAATGCCAAG GAACTGGTTG ATTACTTCCT GAAATTAGCC 
ACAGTCCAGA GTGGGTACTA CCGCACCAAC CACACCATAA TGACCATGGG CTCAGACTTC 
CAGTATGAGA ATGCCAACAT GTGGTTTAAG AACCTTGATA AGCTCATCCA TCTGGTCAAT 
GCTCAGCAGG CCAATGGGAG CCACGTCAAT GTTCTCTACT CCACTCCGGC CTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA CCTCACCTGG TCGGCGAAAC AGGATGACTT CTTCCCCTAC 
GCCGATGGCC CCCACCAGTT CTGGACAGGC TTCTTTTCCA GCCGGCCGGG CCTCAAACGC 
TACGAGCGTC TCAGCTATAA CTTCCTGCAG GTGTGCAACC AGCTGGAGGC GCTGGCAGGT 
CCGGCGGCCA ACGTGGGACC TTATGGCTCG GGAGACAGCG CACCCCTCAA TGAGGCGATG 
GCTGTGCTCC AGCACCATGA TGCGGTCAGT GGTACCTCCC GGCAGCACGT GGCAGACGAC 
TATGCTCGCC AGCTTGCCGC AGGCTGGGGA CCCTGTGAGG TTCTCCTGAG TAACGCGCTG 
GCACGGCTCA GCGGCTCCAA GGAGGAATTT GCGTTCTGCC GCTGGCTCAA CATCAGCGTC 
TGTCCGCTTA GCCAGACCGC GGAGATCTTC CAGGTGACTA TTTATAACCC CTTGGGGCGG 
AAAGTGGAAT GGATGGTGCG GCTTCCGGTC AGCAAACACA TATTCCTCGT GAAAGATCCC 
AATGGCACAG TTATACCCAG CGATGTGGTT ACAATGCCCA GCTCAGACAG ACAGGAGCTG 
CTTTTCTCAG CCTCAGTGCC TGCCCTCGGC TTCAGCATCT ACTCAGTAAC CCAGGTGCCT 
GGCCAGAGCC CACAGGCTCA CACCTCCAAG CCCAGATCCC AGAAGTCCTC GTCCCGCATC 
TTGACCATCC AAAATGAGTA CATCCGGGCT AGATTTGATC CTGACACAGG GCTCTTGGTG 
GAGTTGGAGA ACATGGACCA GAAACTCCTG CTGCCTGTTC GCCAAGCCTT CTACTGGTAC 
AACGCCAGTA CAGGCAACTA CCTAAGTGAC CAGGTCTCAG GTGCCTACAT CTTCAGACCC 
AACCGACAGG AACCACTGCT CGTGAGCCGC TGGGCTCAAA TCCACCTGGT GAAGAAGGCC 
TTGGTGCAGG AGGTACACCA GAACTTCTCA GCCTGGTGTT CCCAAGTGGT TCGTCTGTAT 
CCAGGACAGC GGCACCTGGA GCTGGAGTGG ACAGTGGGAC CAATACCTGT GGGTGATGGC 
TGGGGAAAGG AGGTCATTAG CCGTTTTGAC ACTGAGCTGG AGACAAAAGG ACTCTTCTTC 
ACTGACAGCA ATGGCCGGGA GATCCTGGAG AGAAGACGGA ATTATCGACC CACATGGGTG 
CTGAACATGA CTGAGCCAGT GGCAGGAAAC TACTATCCAG TCAACAGTCG CATTTACATT 
ACGGACGGGA AAGCGCAACT GACGGTGCTG ACAGACCGTT CCCAGGGCGG CAGCAGCCTG 
AAAGATGGCT CCGTAGAGCT CATGGTGCAC CGAAGGCTGC TGAAAGACGA TAGGCGCGGA 
GTTGGGGAGC CACTGCTGGA GAATGGGTCG GGGTCGGGGT TGTGGGTGCG GGGGCGCCAC 
CTCGTGCTGC TGGATAAGGC CCGGACCGCC GCTGTCAGGC ACCGGTTGCA GGCGGAGAAG 
GAGCTCCTGG CCCCGCAGGT GGTGCTGGCC CAGGGTAGCG GCACTCCCTA CCACCCGGGG 
GTCACCCCGC GCAGGCAGTT CTCCGGGCTG CGCAGGGAGC TGCCGCCCAA CGTGCACCTG 
CTTACCCTCG CCCGCTGGGG CCGGGAAACG CTGCTGCTGC GCTTAGAGCA TCAGTTCGCC 
GTACAGGAGG ATATGGTCGG CAACTTGAGC TCTCCGGTGA CCTTGGACTT GAGGAACCTG 
TTTTCTGCCT TCACCATCAC TGACCTTAAG GAAACCACGC TTGCTGCCAA CCAGCTACGG 
GCCAGTGCCT CCAGGCTCCA GTGGACAGTG AACACGGATG AGGGCCACAC ACCCAAGTCC 
TCTCCCTTCC AACTGGACCC TGCCAGTGTC ACACTGCAGC CCATGGAAAT CCGCACCTTC 
CTGGCCTCAG TCCAGTGGGA AAAGGACAGC TAG