Rest cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rest
Entrez Gene ID	83618
Full Name	RE1-silencing transcription factor
Synonyms	Nrsf
General protein information	Preferred Names RE1-silencing transcription factor Names RE1-silencing transcription factor neural-restrictive silencer factor zinc finger transcription factor REST protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p11
Summary	represses transcription of the AMPA receptor (AMPAR) subunit GluR2 and other neural genes [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006250848.1	XP_006250910.1	RE1-silencing transcription factor isoform X1
NM_031788.1	NP_113976.1	RE1-silencing transcription factor
XM_006250849.3	XP_006250911.1	RE1-silencing transcription factor isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Rest	NP_113976.1
Bos taurus (cattle)	REST	XP_005208055.1
Gallus gallus (chicken)	REST	XP_420580.4
Pan troglodytes (chimpanzee)	REST	XP_003310418.1
Canis lupus familiaris (dog)	REST	XP_539282.3
Homo sapiens (human)	REST	NP_001180437.1
	REST	XP_002804152.1
Mus musculus (house mouse)	Rest	NP_035393.2

Gene Ontology
Bibliography

Related articles in PubMed

Role of the Astroglial Glutamate Exchanger xCT in Ventral Hippocampus in Resilience to Stress.
Nasca C, Bigio B, Zelli D, de Angelis P, Lau T, Okamoto M, Soya H, Ni J, Brichta L, Greengard P, Neve RL, Lee FS, McEwen BS
Neuron96(2)402-413.e5(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Authors demonstrate for the first time novel neuroprotective function of REST in prion diseases and hypothesise that the LRP6-Wnt-beta-catenin/REST signaling plays critical and collaborative roles in neuroprotection.
Title: REST alleviates neurotoxic prion peptide-induced synaptic abnormalities, neurofibrillary degeneration and neuronal death partially via LRP6-mediated Wnt-?-catenin signaling.

miR-20, Rest and Wnt signaling are suggested to be involved in a regulatory circuit that can modulate the neural differention of neural progenitor cells. [mir-20]
Title: The miR-20-Rest-Wnt signaling axis regulates neural progenitor cell differentiation.

These effects of FosDT in part are due to its interactions with corepressors of the transcription factor REST and subsequent derepression of REST-downstream genes
Title: Long Noncoding RNA FosDT Promotes Ischemic Brain Injury by Interacting with REST-Associated Chromatin-Modifying Proteins.

Results indicate that neuron-restrictive silencer factor plays an important role as a repressor of VGF gene regulation in neuroblastoma cells through a mechanism that is dependent on VGF-neuron-restrictive silencer element
Title: Transcriptional regulation of the neuropeptide VGF by the neuron-restrictive silencer factor/neuron-restrictive silencer element.

The transcription factor NRSF contributes to epileptogenesis by selective repression of a subset of target genes.
Title: The transcription factor NRSF contributes to epileptogenesis by selective repression of a subset of target genes.

The following Rest gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rest cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08743	NM_031788.1 Latest version!	Rattus norvegicus RE1-silencing transcription factor (Rest), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$783.30 $1119.00
ORa35991	XM_006250849.3 Latest version!	Rattus norvegicus RE1-silencing transcription factor (Rest), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
ORa35991	XM_006250848.1 Latest version!	Rattus norvegicus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rest ( NM_031788.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113976.1 Rattus norvegicus RE1-silencing transcription factor (Rest), mRNA.

Price & Availability↑

CloneID	ORa08743
Clone ID Related Accession (Same CDS sequence)	NM_031788.1
Accession Version	NM_031788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	RE1-silencing transcription factor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF037199.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF037199.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGAA GTCTCTTTAA CAACAGTGGC 
AACATGGGCA TGGCCTTACC CAACGACATG TATGACTTGC ACGACCTCTC GAAAGCTGAA 
CTGGCGGCAC CTCAGCTCAT TATGTTAGCC AACGTGGCCC TGACTGGGGA AGTGAATGGC 
AGCTGCTGTG ATTACCTGGT TGGTGAAGAG AGACAGATGG CCGAGTTGAT GCCTGTTGGA 
GACAACCACT TTTCAGATAG CGAAGGAGAA GGCCTTGAGG AGTCGGCTGA ACTAAAAGGT 
GACCCCAGTG GGCTGGACAA CATGGAACTG AGAAGTTTGG AGCTAAGCGT TGTAGAGCCC 
CAGCCCGTAT TTGAAGCATC AGCTGCCCCA GAAGTGTACA GCTCGAATAA AGATCCCGCC 
CCTGAAGCAC CCGTGGCGGA GGACAAATGC AAGAATTTGA AGGCCAAACC CTTCCGTTGT 
AAGCCATGCC AGTATGAAGC GGAGTCTGAA GAACAGTTCG TACATCACAT CCGGGTTCAC 
AGTGCTAAGA AGTTTTTTGT GGAAGAGAGT GCAGAGAAGC AAGCCAAAGC CAGGGAATCT 
GGGGCTTCCC CGTCTGAGGA GGGCGAGTTC TCCAAGGGTC CCATCCGCTG TGATCGCTGT 
GGCTACAATA CCAACCGGTA TGATCACTAC ACGGCACACC TGAAGCACCA CCTGAGAGCC 
GGGGATAACG AGCGTGTCTA CAAGTGTATC ATTTGCACGT ACACGACAGT CAGCGAATAC 
CACTGGCGGA AACACCTGAG GAACCATTTT CCCAGGAAAG TCTACACGTG TAGCAAGTGC 
AACTATTTTT CGACAGAAAA AAATAATTAT GTTCAACACG TTCGAACTCA CACAGGAGAA 
CGCCCTTATA AATGTGAACT GTGTCCTTAC TCAAGTTCTC AGAAGACTCA TCTAACTCGA 
CACATGCGTA CTCACTCAGG TGAGAAGCCA TTTAAATGTG ATCAGTGCAA TTATGTGGCC 
TCTAATCAGC ACGAAGTGAC CCGACACGCA AGACAGGTTC ACAACGGGCC TAAACCTCTT 
AATTGCCCTC ACTGTGACTA CAAAACAGCC GATAGGAGCA ACTTCAAGAA GCACGTCGAG 
CTGCACGTCA ACCCTCGGCA GTTCAACTGC CCCGTGTGTG ACTACGCGGC CTCCAAGAAG 
TGTAACCTGC AGTACCATTT CAAGTCCAAG CACCCCACCT GCCCCAGCAA GACGATGGAC 
GTGTCCAAAG TGAAGCTGAA GAAAACCAAG AGGAGGGAGG CTGACCTGCA CCGTGACGCC 
GCCGCCGCCG CCACTGAGCA GACGGACACA GAGCAAGCGA AAACCAAGGG GGTGGACGCG 
TCTGCGAGGA GAAGTGAGAG GCCTGTAAAA GGCGTTGGAA AAGATGTTCC AAAAGAGAAG 
AAGCCCTGTA GCAATGCCTC TGTGGTGCAG GTAACTACCC GAACTCGGAA ATCAGCGGTG 
GAGACTAAAG CAGCGGAGGG AAAACACACA GATGGACAGA CAGGAAACAA CGCAGAAAAG 
TCCTCTAAAG CTAAGAAGAG CAAAAGGAAG ATGGACGCCG AGGCCCATCC CTCGGTCGAG 
CCTGTGACTG AGGGACCCGT GACAAAGAAG AAAAAGACGG AGAGCAAACC CAAGACCAGC 
GGCGAAGTGC CGAAGGGCAG CAGAGTGGAG GACAGGAAGG CGGACAAACA GCAAAGTGCT 
TCCATTAAGA AAGGCGGGAA GAAGACGGCT CTCAAGACTA AGACAGCTAA AAAAGGCAGC 
AAACTTGCTC CGAAGTGGGT GGGGCACACA GAACCTTCCT CGGAGATGGC TCAAGGAGGG 
GAGTCTCCAG TTCCTGCTCT CACTCAGGCG GTGGTCACCC CATCAGGATC TACTCAGACA 
GAGCTCTCTT CTCCCATGGA TATTGCTCAG ACAGAGCCTG CCCAGATGGA CGTTTCCCAG 
ACAGGGCCGC CTCAGGTGCA GCGGCCTCTT CCTGTGGAGC CTGCTCAATT GGAGCCGTCT 
CCTCCTCAGG AGCCTCCCCA GGTAGAGCCA CCTGCCTGTG TGGAGCCTCC CCCTCCCGTG 
GAGCCTCCAT GTCCCATGGA GCCTGCTGAG ATGGAACCGT CCCCTCCCAT GGAGCCTTCC 
CAGGTGGAGC CACCTCCTCA TTTGGAGCCT CCGCTTCCCA TGGAGCTGCC TCAGGTGGAG 
CTGCCTCCTG TGGAGGATTG TCAGAAGGAG CTGCCTCCTG TGGAGCATGC TCAGACTAAG 
GTTGCTCAGA CAGGTCCTAC TCAGGTGGGA GCTGTTCAGG AGGAGCCCCT TTTCTGTCTC 
CGAGCCACCT CAAGTCAAGC TAACCAGAAG GTCATCTCCC CGAAAGACCG TGCCAAGGAG 
AAGTTGAGCG TGCTGAGTGA GATGGCGAGG CAGGAGCAGG TTCTTATTGA GGTTGGCTTA 
GTGCCTGTCA GAGATAGCCA GCTTCTGAAG GCCAGCAAGA GCGCACCGGA CCTCCCAGCC 
CCACCGTCAC CACTGCCAAA GGGACACTTG AGAAGAGAAG AGACACCCAA GGACCAAGAA 
ATGTTCTCTG ACGGGGAAGG AAATAAAGTA TCCCCTCTCG AGAAAGGAGG AACAGAGGAA 
GCTGGTGAGA GTCGAGCTGA GCTGGCTGCT CCCATGGAAT CTACCAGTGC TTTATCCTCT 
GAACAAAGCT CAAATGCACC AGATGGTGAA ACATTACACA GCGAGTGTCA GGCTGACTCC 
ACTGCGGTTT GTGAAATGGA AGTGGACACT GAGCAGAAGA CAGACCGTGT CCCTCTGAAA 
GACTCAGCAG TAGAACCAGT GTCACCTCTT AACCCAAGAG TGGACCCTGA AGCAGCGGCA 
CCAGCTGTGG TGGCCTCCCC TCCTATCACT TTGGCCGAGT CTCAGGAAAT TGATGAGGAT 
GAAGGCATTC ACAGCCATGA TGGAAGTGAC CTGAGCGACA ACATGTCTGA GGGGAGTGAT 
GACTCAGGAC TGCATGGGGC TCGGCCAGCA CCACAGGAAG CTACGTCAAA AAGTGGAAAG 
GAAGGGTTGG CTGTCAAAGT AACTGAGGGA GAGTTTGTTT GTATTTTTTG TGATCGTTCT 
TTTAGAAAGG AAAAAGACTA TAGCAAACAC CTCAATCGCC ATTTGGTTAA TGTGTACTTC 
CTTGAAGAAG CAGCTGAGGA GCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113976.1
CDS	1..3210
Translation	MATQVMGQSSGGGSLFNNSGNMGMALPNDMYDLHDLSKAELAAPQLIMLANVALTGEVNGSCCDYLVGEERQMAELMPVGDNHFSDSEGEGLEESAELKGDPSGLDNMELRSLELSVVEPQPVFEASAAPEVYSSNKDPAPEAPVAEDKCKNLKAKPFRCKPCQYEAESEEQFVHHIRVHSAKKFFVEESAEKQAKARESGASPSEEGEFSKGPIRCDRCGYNTNRYDHYTAHLKHHLRAGDNERVYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCSKCNYFSTEKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCNYVASNQHEVTRHARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPSKTMDVSKVKLKKTKRREADLHRDAAAAATEQTDTEQAKTKGVDASARRSERPVKGVGKDVPKEKKPCSNASVVQVTTRTRKSAVETKAAEGKHTDGQTGNNAEKSSKAKKSKRKMDAEAHPSVEPVTEGPVTKKKKTESKPKTSGEVPKGSRVEDRKADKQQSASIKKGGKKTALKTKTAKKGSKLAPKWVGHTEPSSEMAQGGESPVPALTQAVVTPSGSTQTELSSPMDIAQTEPAQMDVSQTGPPQVQRPLPVEPAQLEPSPPQEPPQVEPPACVEPPPPVEPPCPMEPAEMEPSPPMEPSQVEPPPHLEPPLPMELPQVELPPVEDCQKELPPVEHAQTKVAQTGPTQVGAVQEEPLFCLRATSSQANQKVISPKDRAKEKLSVLSEMARQEQVLIEVGLVPVRDSQLLKASKSAPDLPAPPSPLPKGHLRREETPKDQEMFSDGEGNKVSPLEKGGTEEAGESRAELAAPMESTSALSSEQSSNAPDGETLHSECQADSTAVCEMEVDTEQKTDRVPLKDSAVEPVSPLNPRVDPEAAAPAVVASPPITLAESQEIDEDEGIHSHDGSDLSDNMSEGSDDSGLHGARPAPQEATSKSGKEGLAVKVTEGEFVCIFCDRSFRKEKDYSKHLNRHLVNVYFLEEAAEEQE

Target ORF information:

RefSeq Version	NM_031788.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RE1-silencing transcription factor (Rest), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031788.1

ORF Insert Sequence:

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGAA GTCTCTTTAA CAACAGTGGC 
AACATGGGCA TGGCCTTACC CAACGACATG TATGACTTGC ACGACCTCTC GAAAGCTGAA 
CTGGCGGCAC CTCAGCTCAT TATGTTAGCC AACGTGGCCC TGACTGGGGA AGTGAATGGC 
AGCTGCTGTG ATTACCTGGT TGGTGAAGAG AGACAGATGG CCGAGTTGAT GCCTGTTGGA 
GACAACCACT TTTCAGATAG CGAAGGAGAA GGCCTTGAGG AGTCGGCTGA ACTAAAAGGT 
GACCCCAGTG GGCTGGACAA CATGGAACTG AGAAGTTTGG AGCTAAGCGT TGTAGAGCCC 
CAGCCCGTAT TTGAAGCATC AGCTGCCCCA GAAGTGTACA GCTCGAATAA AGATCCCGCC 
CCTGAAGCAC CCGTGGCGGA GGACAAATGC AAGAATTTGA AGGCCAAACC CTTCCGTTGT 
AAGCCATGCC AGTATGAAGC GGAGTCTGAA GAACAGTTCG TACATCACAT CCGGGTTCAC 
AGTGCTAAGA AGTTTTTTGT GGAAGAGAGT GCAGAGAAGC AAGCCAAAGC CAGGGAATCT 
GGGGCTTCCC CGTCTGAGGA GGGCGAGTTC TCCAAGGGTC CCATCCGCTG TGATCGCTGT 
GGCTACAATA CCAACCGGTA TGATCACTAC ACGGCACACC TGAAGCACCA CCTGAGAGCC 
GGGGATAACG AGCGTGTCTA CAAGTGTATC ATTTGCACGT ACACGACAGT CAGCGAATAC 
CACTGGCGGA AACACCTGAG GAACCATTTT CCCAGGAAAG TCTACACGTG TAGCAAGTGC 
AACTATTTTT CGACAGAAAA AAATAATTAT GTTCAACACG TTCGAACTCA CACAGGAGAA 
CGCCCTTATA AATGTGAACT GTGTCCTTAC TCAAGTTCTC AGAAGACTCA TCTAACTCGA 
CACATGCGTA CTCACTCAGG TGAGAAGCCA TTTAAATGTG ATCAGTGCAA TTATGTGGCC 
TCTAATCAGC ACGAAGTGAC CCGACACGCA AGACAGGTTC ACAACGGGCC TAAACCTCTT 
AATTGCCCTC ACTGTGACTA CAAAACAGCC GATAGGAGCA ACTTCAAGAA GCACGTCGAG 
CTGCACGTCA ACCCTCGGCA GTTCAACTGC CCCGTGTGTG ACTACGCGGC CTCCAAGAAG 
TGTAACCTGC AGTACCATTT CAAGTCCAAG CACCCCACCT GCCCCAGCAA GACGATGGAC 
GTGTCCAAAG TGAAGCTGAA GAAAACCAAG AGGAGGGAGG CTGACCTGCA CCGTGACGCC 
GCCGCCGCCG CCACTGAGCA GACGGACACA GAGCAAGCGA AAACCAAGGG GGTGGACGCG 
TCTGCGAGGA GAAGTGAGAG GCCTGTAAAA GGCGTTGGAA AAGATGTTCC AAAAGAGAAG 
AAGCCCTGTA GCAATGCCTC TGTGGTGCAG GTAACTACCC GAACTCGGAA ATCAGCGGTG 
GAGACTAAAG CAGCGGAGGG AAAACACACA GATGGACAGA CAGGAAACAA CGCAGAAAAG 
TCCTCTAAAG CTAAGAAGAG CAAAAGGAAG ATGGACGCCG AGGCCCATCC CTCGGTCGAG 
CCTGTGACTG AGGGACCCGT GACAAAGAAG AAAAAGACGG AGAGCAAACC CAAGACCAGC 
GGCGAAGTGC CGAAGGGCAG CAGAGTGGAG GACAGGAAGG CGGACAAACA GCAAAGTGCT 
TCCATTAAGA AAGGCGGGAA GAAGACGGCT CTCAAGACTA AGACAGCTAA AAAAGGCAGC 
AAACTTGCTC CGAAGTGGGT GGGGCACACA GAACCTTCCT CGGAGATGGC TCAAGGAGGG 
GAGTCTCCAG TTCCTGCTCT CACTCAGGCG GTGGTCACCC CATCAGGATC TACTCAGACA 
GAGCTCTCTT CTCCCATGGA TATTGCTCAG ACAGAGCCTG CCCAGATGGA CGTTTCCCAG 
ACAGGGCCGC CTCAGGTGCA GCGGCCTCTT CCTGTGGAGC CTGCTCAATT GGAGCCGTCT 
CCTCCTCAGG AGCCTCCCCA GGTAGAGCCA CCTGCCTGTG TGGAGCCTCC CCCTCCCGTG 
GAGCCTCCAT GTCCCATGGA GCCTGCTGAG ATGGAACCGT CCCCTCCCAT GGAGCCTTCC 
CAGGTGGAGC CACCTCCTCA TTTGGAGCCT CCGCTTCCCA TGGAGCTGCC TCAGGTGGAG 
CTGCCTCCTG TGGAGGATTG TCAGAAGGAG CTGCCTCCTG TGGAGCATGC TCAGACTAAG 
GTTGCTCAGA CAGGTCCTAC TCAGGTGGGA GCTGTTCAGG AGGAGCCCCT TTTCTGTCTC 
CGAGCCACCT CAAGTCAAGC TAACCAGAAG GTCATCTCCC CGAAAGACCG TGCCAAGGAG 
AAGTTGAGCG TGCTGAGTGA GATGGCGAGG CAGGAGCAGG TTCTTATTGA GGTTGGCTTA 
GTGCCTGTCA GAGATAGCCA GCTTCTGAAG GCCAGCAAGA GCGCACCGGA CCTCCCAGCC 
CCACCGTCAC CACTGCCAAA GGGACACTTG AGAAGAGAAG AGACACCCAA GGACCAAGAA 
ATGTTCTCTG ACGGGGAAGG AAATAAAGTA TCCCCTCTCG AGAAAGGAGG AACAGAGGAA 
GCTGGTGAGA GTCGAGCTGA GCTGGCTGCT CCCATGGAAT CTACCAGTGC TTTATCCTCT 
GAACAAAGCT CAAATGCACC AGATGGTGAA ACATTACACA GCGAGTGTCA GGCTGACTCC 
ACTGCGGTTT GTGAAATGGA AGTGGACACT GAGCAGAAGA CAGACCGTGT CCCTCTGAAA 
GACTCAGCAG TAGAACCAGT GTCACCTCTT AACCCAAGAG TGGACCCTGA AGCAGCGGCA 
CCAGCTGTGG TGGCCTCCCC TCCTATCACT TTGGCCGAGT CTCAGGAAAT TGATGAGGAT 
GAAGGCATTC ACAGCCATGA TGGAAGTGAC CTGAGCGACA ACATGTCTGA GGGGAGTGAT 
GACTCAGGAC TGCATGGGGC TCGGCCAGCA CCACAGGAAG CTACGTCAAA AAGTGGAAAG 
GAAGGGTTGG CTGTCAAAGT AACTGAGGGA GAGTTTGTTT GTATTTTTTG TGATCGTTCT 
TTTAGAAAGG AAAAAGACTA TAGCAAACAC CTCAATCGCC ATTTGGTTAA TGTGTACTTC 
CTTGAAGAAG CAGCTGAGGA GCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rest ( XM_006250848.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250910.1 Rattus norvegicus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35991
Clone ID Related Accession (Same CDS sequence)	XM_006250848.1 , XM_006250849.3
Accession Version	XM_006250848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	RE1-silencing transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGAA GTCTCTTTAA CAACAGTGGC 
AACATGGGCA TGGCCTTACC CAACGACATG TATGACTTGC ACGACCTCTC GAAAGCTGAA 
CTGGCGGCAC CTCAGCTCAT TATGTTAGCC AACGTGGCCC TGACTGGGGA AGTGAATGGC 
AGCTGCTGTG ATTACCTGGT TGGTGAAGAG AGACAGATGG CCGAGTTGAT GCCTGTTGGA 
GACAACCACT TTTCAGATAG CGAAGGAGAA GGCCTTGAGG AGTCGGCTGA ACTAAAAGGT 
GACCCCAGTG GGCTGGACAA CATGGAACTG AGAAGTTTGG AGCTAAGCGT TGTAGAGCCC 
CAGCCTGTAT TTGAAGCATC AGCTGCCCCA GAAGTGTACA GCTCGAATAA AGATCCCGCC 
CCTGAAGCAC CCGTGGCGGA GGACAAATGC AAGAATTTGA AGGCCAAACC CTTCCGTTGT 
AAGCCATGCC AGTATGAAGC GGAGTCTGAA GAACAGTTCG TACATCACAT CCGGGTTCAC 
AGTGCTAAGA AGTTTTTTGT GGAAGAGAGT GCAGAGAAGC AAGCCAAAGC CAGGGAATCT 
GGGGCTTCCC CGTCTGAGGA GGGCGAGTTC TCCAAGGGTC CCATCCGCTG TGATCGCTGT 
GGCTACAATA CCAACCGGTA TGATCACTAC ACGGCACACC TGAAGCACCA CCTGAGAGCC 
GGGGATAACG AGCGTGTCTA CAAGTGTATC ATTTGCACGT ACACGACGGT CAGCGAATAC 
CACTGGCGGA AACACCTGAG GAACCATTTT CCCAGGAAAG TCTACACGTG TAGCAAGTGC 
AACTATTTTT CCGACAGAAA AAATAATTAT GTTCAACACG TTCGAACTCA CACAGGAGAA 
CGCCCTTATA AATGTGAACT GTGTCCTTAC TCAAGTTCTC AGAAGACTCA TCTAACTCGA 
CACATGCGTA CTCACTCAGG TGAGAAGCCA TTTAAATGTG ATCAGTGCAA TTATGTGGCC 
TCTAATCAGC ACGAAGTGAC CCGACACGCA AGACAGGTTC ACAACGGGCC TAAACCTCTT 
AATTGCCCTC ACTGTGACTA CAAAACAGCC GATAGGAGCA ACTTCAAGAA GCACGTCGAG 
CTGCACGTCA ACCCTCGGCA GTTCAACTGC CCCGTGTGTG ACTACGCGGC CTCCAAGAAG 
TGTAACCTGC AGTACCATTT CAAGTCCAAG CACCCCACCT GCCCCAGCAA GACGATGGAC 
GTGTCCAAAG TGAAGCTGAA GAAAACCAAG AGGAGGGAGG CTGACCTGCA CCGTGACGCC 
GCCGCCGCCG CCACTGAGCA GACGGACACA GAGCAAGCGA AAACCAAGGG GGTGGACGCG 
TCTGCGAGGA GAAGTGAGAG GCCTGTAAAA GGCGTTGGAA AAGATGTTCC AAAAGAGAAG 
AAGCCCTGTA GCAATGCCTC TGTGGTGCAG GTAACTACCC GAACTCGGAA ATCAGCGGTG 
GAGACTAAAG CAGCGGAGGG AAAACACACA GATGGACAGA CAGGAAACAA CGCAGAAAAG 
TCCTCTAAAG CTAAGAAGAG CAAAAGGAAG ATGGACGCCG AGGCCCATCC CTCGGCTGAA 
CCTGTGACTG AGGGACCCGT GACAAAGAAG AAAAAGACGG AGAGCAAACC CAAGACCAGC 
GGCGAAGTGC CGAAGGGCAG CAGAGTGGAG GACAGGAAGG CGGACAAACA GCAAAGTGCT 
TCCATTAAGA AAGGCGGGAA GAAGACGGCT CTCAAGACTA AGACAGCTAA AAAAGGCAGC 
AAACTTGCTC CTAAGGGGGT GGGGCACACA GAACCTTCCT CGGAGATGGC TCAAGGAGGG 
GAGTCTCCAG TTCCTGCTCT CACTCAGGCG GTGGTCACCC CATCAGGATC TACTCAGACA 
GAGCTCTCTT CTCCCATGGA TATTGCTCAG ACAGAGCCTG CCCAGATGGA CGTTTCCCAG 
ACAGGGCCGC CTCAGGTGCA GCGGCCTCTT CCTGTGGAGC CTGCTCAATT GGAGCCGTCT 
CCTCCTCAGG AGCCTCCCCA GGTAGAGCCA CCTGCCTGTG TGGAGCCTCC CCCTCCCGTG 
GAGCCTCCAT GTCCCATGGA GCCTGCTGAG ATGGAACCGT CCCCTCCCAT GGAGCCTTCC 
CAGGTGGAGC CACCTCCTCA TTTGGAGCCT CCGCTTCCCA TGGAGCTGCC TCAGGTGGAG 
CTGCCTCCTG TGGAGGATTG TCAGAAGGAG CTGCCTTCTG TGGAGCATGC TCAGACTAAG 
GTTGCTCAGA CAGGTCCTAC TCAGGTGGGA GCTGTTCAGG AGGAGCCCCT TTCTGTCTCC 
GAGCCACCTC AAGTCAAGCT AACCAGAAGG TCATCTCCCC GGAAAGACCG TGCCAAGGAG 
AAGTTGAGCG TGCTGAGTGA GATGGCGAGG CAGGAGCAGG TTCTTATTGA GGTTGGCTTA 
GTGCCTGTCA GAGATAGCCA GCTTCTGAAG GCCAGCAAGA GCGCACCGGA CCTCCCAGCC 
CCACCGTCAC CACTGCCAAA GGGACACTTG AGAAGAGAAG AGACACCCAA GGACCAAGAA 
ATGTTCTCTG ACGGGGAAGG AAATAAAGTA TCCCCTCTCG AGAAAGGAGG AACAGAGGAA 
GCTGGTGAGA GTCGAGCTGA GCTGGCTGCT CCCATGGAAT CTACCAGTGC TTTATCCTCT 
GAACAAAGCT CAAATGCACC AGATGGTGAA ACATTACACA GCGAGTGTCA GGCTGACTCC 
ACTGCGGTTT GTGAAATGGA AGTGGACACT GAGCAGAAGA CAGACCGTGT CCCTCTGAAA 
GACTCAGCAG TAGAACCAGT GTCACCTCTT AACCCAAGAG TGGACCCTGA AGCAGCGGCA 
CCAGCTGTGG TGGCCTCCCC TCCTATCACT TTGGCCGAGT CTCAGGAAAT TGATGAGGAT 
GAAGGCATTC ACAGCCATGA TGGAAGTGAC CTGAGCGACA ACATGTCTGA GGGGAGTGAT 
GACTCAGGAC TGCATGGGGC TCGGCCAGCA CCACAGGAAG CTACGTCAAA AAGTGGAAAG 
GAAGGGTTGG CTGTCAAAGT AACTGAGGGA GAGTTTGTTT GTATTTTTTG TGATCGTTCT 
TTTAGAAAGG AAAAAGACTA TAGCAAACAC CTCAATCGCC ATTTGGTGAA TGTGTACTTC 
CTTGAAGAAG CAGCTGAGGA GCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250910.1
CDS	85..3294
Translation	MATQVMGQSSGGGSLFNNSGNMGMALPNDMYDLHDLSKAELAAPQLIMLANVALTGEVNGSCCDYLVGEERQMAELMPVGDNHFSDSEGEGLEESAELKGDPSGLDNMELRSLELSVVEPQPVFEASAAPEVYSSNKDPAPEAPVAEDKCKNLKAKPFRCKPCQYEAESEEQFVHHIRVHSAKKFFVEESAEKQAKARESGASPSEEGEFSKGPIRCDRCGYNTNRYDHYTAHLKHHLRAGDNERVYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCSKCNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCNYVASNQHEVTRHARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPSKTMDVSKVKLKKTKRREADLHRDAAAAATEQTDTEQAKTKGVDASARRSERPVKGVGKDVPKEKKPCSNASVVQVTTRTRKSAVETKAAEGKHTDGQTGNNAEKSSKAKKSKRKMDAEAHPSAEPVTEGPVTKKKKTESKPKTSGEVPKGSRVEDRKADKQQSASIKKGGKKTALKTKTAKKGSKLAPKGVGHTEPSSEMAQGGESPVPALTQAVVTPSGSTQTELSSPMDIAQTEPAQMDVSQTGPPQVQRPLPVEPAQLEPSPPQEPPQVEPPACVEPPPPVEPPCPMEPAEMEPSPPMEPSQVEPPPHLEPPLPMELPQVELPPVEDCQKELPSVEHAQTKVAQTGPTQVGAVQEEPLSVSEPPQVKLTRRSSPRKDRAKEKLSVLSEMARQEQVLIEVGLVPVRDSQLLKASKSAPDLPAPPSPLPKGHLRREETPKDQEMFSDGEGNKVSPLEKGGTEEAGESRAELAAPMESTSALSSEQSSNAPDGETLHSECQADSTAVCEMEVDTEQKTDRVPLKDSAVEPVSPLNPRVDPEAAAPAVVASPPITLAESQEIDEDEGIHSHDGSDLSDNMSEGSDDSGLHGARPAPQEATSKSGKEGLAVKVTEGEFVCIFCDRSFRKEKDYSKHLNRHLVNVYFLEEAAEEQE

Target ORF information:

RefSeq Version	XM_006250848.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RE1-silencing transcription factor (Rest), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250848.1

ORF Insert Sequence:

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGAA GTCTCTTTAA CAACAGTGGC 
AACATGGGCA TGGCCTTACC CAACGACATG TATGACTTGC ACGACCTCTC GAAAGCTGAA 
CTGGCGGCAC CTCAGCTCAT TATGTTAGCC AACGTGGCCC TGACTGGGGA AGTGAATGGC 
AGCTGCTGTG ATTACCTGGT TGGTGAAGAG AGACAGATGG CCGAGTTGAT GCCTGTTGGA 
GACAACCACT TTTCAGATAG CGAAGGAGAA GGCCTTGAGG AGTCGGCTGA ACTAAAAGGT 
GACCCCAGTG GGCTGGACAA CATGGAACTG AGAAGTTTGG AGCTAAGCGT TGTAGAGCCC 
CAGCCTGTAT TTGAAGCATC AGCTGCCCCA GAAGTGTACA GCTCGAATAA AGATCCCGCC 
CCTGAAGCAC CCGTGGCGGA GGACAAATGC AAGAATTTGA AGGCCAAACC CTTCCGTTGT 
AAGCCATGCC AGTATGAAGC GGAGTCTGAA GAACAGTTCG TACATCACAT CCGGGTTCAC 
AGTGCTAAGA AGTTTTTTGT GGAAGAGAGT GCAGAGAAGC AAGCCAAAGC CAGGGAATCT 
GGGGCTTCCC CGTCTGAGGA GGGCGAGTTC TCCAAGGGTC CCATCCGCTG TGATCGCTGT 
GGCTACAATA CCAACCGGTA TGATCACTAC ACGGCACACC TGAAGCACCA CCTGAGAGCC 
GGGGATAACG AGCGTGTCTA CAAGTGTATC ATTTGCACGT ACACGACGGT CAGCGAATAC 
CACTGGCGGA AACACCTGAG GAACCATTTT CCCAGGAAAG TCTACACGTG TAGCAAGTGC 
AACTATTTTT CCGACAGAAA AAATAATTAT GTTCAACACG TTCGAACTCA CACAGGAGAA 
CGCCCTTATA AATGTGAACT GTGTCCTTAC TCAAGTTCTC AGAAGACTCA TCTAACTCGA 
CACATGCGTA CTCACTCAGG TGAGAAGCCA TTTAAATGTG ATCAGTGCAA TTATGTGGCC 
TCTAATCAGC ACGAAGTGAC CCGACACGCA AGACAGGTTC ACAACGGGCC TAAACCTCTT 
AATTGCCCTC ACTGTGACTA CAAAACAGCC GATAGGAGCA ACTTCAAGAA GCACGTCGAG 
CTGCACGTCA ACCCTCGGCA GTTCAACTGC CCCGTGTGTG ACTACGCGGC CTCCAAGAAG 
TGTAACCTGC AGTACCATTT CAAGTCCAAG CACCCCACCT GCCCCAGCAA GACGATGGAC 
GTGTCCAAAG TGAAGCTGAA GAAAACCAAG AGGAGGGAGG CTGACCTGCA CCGTGACGCC 
GCCGCCGCCG CCACTGAGCA GACGGACACA GAGCAAGCGA AAACCAAGGG GGTGGACGCG 
TCTGCGAGGA GAAGTGAGAG GCCTGTAAAA GGCGTTGGAA AAGATGTTCC AAAAGAGAAG 
AAGCCCTGTA GCAATGCCTC TGTGGTGCAG GTAACTACCC GAACTCGGAA ATCAGCGGTG 
GAGACTAAAG CAGCGGAGGG AAAACACACA GATGGACAGA CAGGAAACAA CGCAGAAAAG 
TCCTCTAAAG CTAAGAAGAG CAAAAGGAAG ATGGACGCCG AGGCCCATCC CTCGGCTGAA 
CCTGTGACTG AGGGACCCGT GACAAAGAAG AAAAAGACGG AGAGCAAACC CAAGACCAGC 
GGCGAAGTGC CGAAGGGCAG CAGAGTGGAG GACAGGAAGG CGGACAAACA GCAAAGTGCT 
TCCATTAAGA AAGGCGGGAA GAAGACGGCT CTCAAGACTA AGACAGCTAA AAAAGGCAGC 
AAACTTGCTC CTAAGGGGGT GGGGCACACA GAACCTTCCT CGGAGATGGC TCAAGGAGGG 
GAGTCTCCAG TTCCTGCTCT CACTCAGGCG GTGGTCACCC CATCAGGATC TACTCAGACA 
GAGCTCTCTT CTCCCATGGA TATTGCTCAG ACAGAGCCTG CCCAGATGGA CGTTTCCCAG 
ACAGGGCCGC CTCAGGTGCA GCGGCCTCTT CCTGTGGAGC CTGCTCAATT GGAGCCGTCT 
CCTCCTCAGG AGCCTCCCCA GGTAGAGCCA CCTGCCTGTG TGGAGCCTCC CCCTCCCGTG 
GAGCCTCCAT GTCCCATGGA GCCTGCTGAG ATGGAACCGT CCCCTCCCAT GGAGCCTTCC 
CAGGTGGAGC CACCTCCTCA TTTGGAGCCT CCGCTTCCCA TGGAGCTGCC TCAGGTGGAG 
CTGCCTCCTG TGGAGGATTG TCAGAAGGAG CTGCCTTCTG TGGAGCATGC TCAGACTAAG 
GTTGCTCAGA CAGGTCCTAC TCAGGTGGGA GCTGTTCAGG AGGAGCCCCT TTCTGTCTCC 
GAGCCACCTC AAGTCAAGCT AACCAGAAGG TCATCTCCCC GGAAAGACCG TGCCAAGGAG 
AAGTTGAGCG TGCTGAGTGA GATGGCGAGG CAGGAGCAGG TTCTTATTGA GGTTGGCTTA 
GTGCCTGTCA GAGATAGCCA GCTTCTGAAG GCCAGCAAGA GCGCACCGGA CCTCCCAGCC 
CCACCGTCAC CACTGCCAAA GGGACACTTG AGAAGAGAAG AGACACCCAA GGACCAAGAA 
ATGTTCTCTG ACGGGGAAGG AAATAAAGTA TCCCCTCTCG AGAAAGGAGG AACAGAGGAA 
GCTGGTGAGA GTCGAGCTGA GCTGGCTGCT CCCATGGAAT CTACCAGTGC TTTATCCTCT 
GAACAAAGCT CAAATGCACC AGATGGTGAA ACATTACACA GCGAGTGTCA GGCTGACTCC 
ACTGCGGTTT GTGAAATGGA AGTGGACACT GAGCAGAAGA CAGACCGTGT CCCTCTGAAA 
GACTCAGCAG TAGAACCAGT GTCACCTCTT AACCCAAGAG TGGACCCTGA AGCAGCGGCA 
CCAGCTGTGG TGGCCTCCCC TCCTATCACT TTGGCCGAGT CTCAGGAAAT TGATGAGGAT 
GAAGGCATTC ACAGCCATGA TGGAAGTGAC CTGAGCGACA ACATGTCTGA GGGGAGTGAT 
GACTCAGGAC TGCATGGGGC TCGGCCAGCA CCACAGGAAG CTACGTCAAA AAGTGGAAAG 
GAAGGGTTGG CTGTCAAAGT AACTGAGGGA GAGTTTGTTT GTATTTTTTG TGATCGTTCT 
TTTAGAAAGG AAAAAGACTA TAGCAAACAC CTCAATCGCC ATTTGGTGAA TGTGTACTTC 
CTTGAAGAAG CAGCTGAGGA GCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rest ( XM_006250849.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250911.1 Rattus norvegicus RE1-silencing transcription factor (Rest), transcript variant X2, mRNA.

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CloneID	ORa35991
Clone ID Related Accession (Same CDS sequence)	XM_006250848.1 , XM_006250849.3
Accession Version	XM_006250849.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	RE1-silencing transcription factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250849.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGAA GTCTCTTTAA CAACAGTGGC 
AACATGGGCA TGGCCTTACC CAACGACATG TATGACTTGC ACGACCTCTC GAAAGCTGAA 
CTGGCGGCAC CTCAGCTCAT TATGTTAGCC AACGTGGCCC TGACTGGGGA AGTGAATGGC 
AGCTGCTGTG ATTACCTGGT TGGTGAAGAG AGACAGATGG CCGAGTTGAT GCCTGTTGGA 
GACAACCACT TTTCAGATAG CGAAGGAGAA GGCCTTGAGG AGTCGGCTGA ACTAAAAGGT 
GACCCCAGTG GGCTGGACAA CATGGAACTG AGAAGTTTGG AGCTAAGCGT TGTAGAGCCC 
CAGCCTGTAT TTGAAGCATC AGCTGCCCCA GAAGTGTACA GCTCGAATAA AGATCCCGCC 
CCTGAAGCAC CCGTGGCGGA GGACAAATGC AAGAATTTGA AGGCCAAACC CTTCCGTTGT 
AAGCCATGCC AGTATGAAGC GGAGTCTGAA GAACAGTTCG TACATCACAT CCGGGTTCAC 
AGTGCTAAGA AGTTTTTTGT GGAAGAGAGT GCAGAGAAGC AAGCCAAAGC CAGGGAATCT 
GGGGCTTCCC CGTCTGAGGA GGGCGAGTTC TCCAAGGGTC CCATCCGCTG TGATCGCTGT 
GGCTACAATA CCAACCGGTA TGATCACTAC ACGGCACACC TGAAGCACCA CCTGAGAGCC 
GGGGATAACG AGCGTGTCTA CAAGTGTATC ATTTGCACGT ACACGACGGT CAGCGAATAC 
CACTGGCGGA AACACCTGAG GAACCATTTT CCCAGGAAAG TCTACACGTG TAGCAAGTGC 
AACTATTTTT CCGACAGAAA AAATAATTAT GTTCAACACG TTCGAACTCA CACAGGAGAA 
CGCCCTTATA AATGTGAACT GTGTCCTTAC TCAAGTTCTC AGAAGACTCA TCTAACTCGA 
CACATGCGTA CTCACTCAGG TGAGAAGCCA TTTAAATGTG ATCAGTGCAA TTATGTGGCC 
TCTAATCAGC ACGAAGTGAC CCGACACGCA AGACAGGTTC ACAACGGGCC TAAACCTCTT 
AATTGCCCTC ACTGTGACTA CAAAACAGCC GATAGGAGCA ACTTCAAGAA GCACGTCGAG 
CTGCACGTCA ACCCTCGGCA GTTCAACTGC CCCGTGTGTG ACTACGCGGC CTCCAAGAAG 
TGTAACCTGC AGTACCATTT CAAGTCCAAG CACCCCACCT GCCCCAGCAA GACGATGGAC 
GTGTCCAAAG TGAAGCTGAA GAAAACCAAG AGGAGGGAGG CTGACCTGCA CCGTGACGCC 
GCCGCCGCCG CCACTGAGCA GACGGACACA GAGCAAGCGA AAACCAAGGG GGTGGACGCG 
TCTGCGAGGA GAAGTGAGAG GCCTGTAAAA GGCGTTGGAA AAGATGTTCC AAAAGAGAAG 
AAGCCCTGTA GCAATGCCTC TGTGGTGCAG GTAACTACCC GAACTCGGAA ATCAGCGGTG 
GAGACTAAAG CAGCGGAGGG AAAACACACA GATGGACAGA CAGGAAACAA CGCAGAAAAG 
TCCTCTAAAG CTAAGAAGAG CAAAAGGAAG ATGGACGCCG AGGCCCATCC CTCGGCTGAA 
CCTGTGACTG AGGGACCCGT GACAAAGAAG AAAAAGACGG AGAGCAAACC CAAGACCAGC 
GGCGAAGTGC CGAAGGGCAG CAGAGTGGAG GACAGGAAGG CGGACAAACA GCAAAGTGCT 
TCCATTAAGA AAGGCGGGAA GAAGACGGCT CTCAAGACTA AGACAGCTAA AAAAGGCAGC 
AAACTTGCTC CTAAGGGGGT GGGGCACACA GAACCTTCCT CGGAGATGGC TCAAGGAGGG 
GAGTCTCCAG TTCCTGCTCT CACTCAGGCG GTGGTCACCC CATCAGGATC TACTCAGACA 
GAGCTCTCTT CTCCCATGGA TATTGCTCAG ACAGAGCCTG CCCAGATGGA CGTTTCCCAG 
ACAGGGCCGC CTCAGGTGCA GCGGCCTCTT CCTGTGGAGC CTGCTCAATT GGAGCCGTCT 
CCTCCTCAGG AGCCTCCCCA GGTAGAGCCA CCTGCCTGTG TGGAGCCTCC CCCTCCCGTG 
GAGCCTCCAT GTCCCATGGA GCCTGCTGAG ATGGAACCGT CCCCTCCCAT GGAGCCTTCC 
CAGGTGGAGC CACCTCCTCA TTTGGAGCCT CCGCTTCCCA TGGAGCTGCC TCAGGTGGAG 
CTGCCTCCTG TGGAGGATTG TCAGAAGGAG CTGCCTTCTG TGGAGCATGC TCAGACTAAG 
GTTGCTCAGA CAGGTCCTAC TCAGGTGGGA GCTGTTCAGG AGGAGCCCCT TTCTGTCTCC 
GAGCCACCTC AAGTCAAGCT AACCAGAAGG TCATCTCCCC GGAAAGACCG TGCCAAGGAG 
AAGTTGAGCG TGCTGAGTGA GATGGCGAGG CAGGAGCAGG TTCTTATTGA GGTTGGCTTA 
GTGCCTGTCA GAGATAGCCA GCTTCTGAAG GCCAGCAAGA GCGCACCGGA CCTCCCAGCC 
CCACCGTCAC CACTGCCAAA GGGACACTTG AGAAGAGAAG AGACACCCAA GGACCAAGAA 
ATGTTCTCTG ACGGGGAAGG AAATAAAGTA TCCCCTCTCG AGAAAGGAGG AACAGAGGAA 
GCTGGTGAGA GTCGAGCTGA GCTGGCTGCT CCCATGGAAT CTACCAGTGC TTTATCCTCT 
GAACAAAGCT CAAATGCACC AGATGGTGAA ACATTACACA GCGAGTGTCA GGCTGACTCC 
ACTGCGGTTT GTGAAATGGA AGTGGACACT GAGCAGAAGA CAGACCGTGT CCCTCTGAAA 
GACTCAGCAG TAGAACCAGT GTCACCTCTT AACCCAAGAG TGGACCCTGA AGCAGCGGCA 
CCAGCTGTGG TGGCCTCCCC TCCTATCACT TTGGCCGAGT CTCAGGAAAT TGATGAGGAT 
GAAGGCATTC ACAGCCATGA TGGAAGTGAC CTGAGCGACA ACATGTCTGA GGGGAGTGAT 
GACTCAGGAC TGCATGGGGC TCGGCCAGCA CCACAGGAAG CTACGTCAAA AAGTGGAAAG 
GAAGGGTTGG CTGTCAAAGT AACTGAGGGA GAGTTTGTTT GTATTTTTTG TGATCGTTCT 
TTTAGAAAGG AAAAAGACTA TAGCAAACAC CTCAATCGCC ATTTGGTGAA TGTGTACTTC 
CTTGAAGAAG CAGCTGAGGA GCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250911.1
CDS	339..3548
Translation	MATQVMGQSSGGGSLFNNSGNMGMALPNDMYDLHDLSKAELAAPQLIMLANVALTGEVNGSCCDYLVGEERQMAELMPVGDNHFSDSEGEGLEESAELKGDPSGLDNMELRSLELSVVEPQPVFEASAAPEVYSSNKDPAPEAPVAEDKCKNLKAKPFRCKPCQYEAESEEQFVHHIRVHSAKKFFVEESAEKQAKARESGASPSEEGEFSKGPIRCDRCGYNTNRYDHYTAHLKHHLRAGDNERVYKCIICTYTTVSEYHWRKHLRNHFPRKVYTCSKCNYFSDRKNNYVQHVRTHTGERPYKCELCPYSSSQKTHLTRHMRTHSGEKPFKCDQCNYVASNQHEVTRHARQVHNGPKPLNCPHCDYKTADRSNFKKHVELHVNPRQFNCPVCDYAASKKCNLQYHFKSKHPTCPSKTMDVSKVKLKKTKRREADLHRDAAAAATEQTDTEQAKTKGVDASARRSERPVKGVGKDVPKEKKPCSNASVVQVTTRTRKSAVETKAAEGKHTDGQTGNNAEKSSKAKKSKRKMDAEAHPSAEPVTEGPVTKKKKTESKPKTSGEVPKGSRVEDRKADKQQSASIKKGGKKTALKTKTAKKGSKLAPKGVGHTEPSSEMAQGGESPVPALTQAVVTPSGSTQTELSSPMDIAQTEPAQMDVSQTGPPQVQRPLPVEPAQLEPSPPQEPPQVEPPACVEPPPPVEPPCPMEPAEMEPSPPMEPSQVEPPPHLEPPLPMELPQVELPPVEDCQKELPSVEHAQTKVAQTGPTQVGAVQEEPLSVSEPPQVKLTRRSSPRKDRAKEKLSVLSEMARQEQVLIEVGLVPVRDSQLLKASKSAPDLPAPPSPLPKGHLRREETPKDQEMFSDGEGNKVSPLEKGGTEEAGESRAELAAPMESTSALSSEQSSNAPDGETLHSECQADSTAVCEMEVDTEQKTDRVPLKDSAVEPVSPLNPRVDPEAAAPAVVASPPITLAESQEIDEDEGIHSHDGSDLSDNMSEGSDDSGLHGARPAPQEATSKSGKEGLAVKVTEGEFVCIFCDRSFRKEKDYSKHLNRHLVNVYFLEEAAEEQE

Target ORF information:

RefSeq Version	XM_006250849.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RE1-silencing transcription factor (Rest), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250849.3

ORF Insert Sequence:

ATGGCCACCC AGGTGATGGG GCAGTCTTCT GGAGGAGGAA GTCTCTTTAA CAACAGTGGC 
AACATGGGCA TGGCCTTACC CAACGACATG TATGACTTGC ACGACCTCTC GAAAGCTGAA 
CTGGCGGCAC CTCAGCTCAT TATGTTAGCC AACGTGGCCC TGACTGGGGA AGTGAATGGC 
AGCTGCTGTG ATTACCTGGT TGGTGAAGAG AGACAGATGG CCGAGTTGAT GCCTGTTGGA 
GACAACCACT TTTCAGATAG CGAAGGAGAA GGCCTTGAGG AGTCGGCTGA ACTAAAAGGT 
GACCCCAGTG GGCTGGACAA CATGGAACTG AGAAGTTTGG AGCTAAGCGT TGTAGAGCCC 
CAGCCTGTAT TTGAAGCATC AGCTGCCCCA GAAGTGTACA GCTCGAATAA AGATCCCGCC 
CCTGAAGCAC CCGTGGCGGA GGACAAATGC AAGAATTTGA AGGCCAAACC CTTCCGTTGT 
AAGCCATGCC AGTATGAAGC GGAGTCTGAA GAACAGTTCG TACATCACAT CCGGGTTCAC 
AGTGCTAAGA AGTTTTTTGT GGAAGAGAGT GCAGAGAAGC AAGCCAAAGC CAGGGAATCT 
GGGGCTTCCC CGTCTGAGGA GGGCGAGTTC TCCAAGGGTC CCATCCGCTG TGATCGCTGT 
GGCTACAATA CCAACCGGTA TGATCACTAC ACGGCACACC TGAAGCACCA CCTGAGAGCC 
GGGGATAACG AGCGTGTCTA CAAGTGTATC ATTTGCACGT ACACGACGGT CAGCGAATAC 
CACTGGCGGA AACACCTGAG GAACCATTTT CCCAGGAAAG TCTACACGTG TAGCAAGTGC 
AACTATTTTT CCGACAGAAA AAATAATTAT GTTCAACACG TTCGAACTCA CACAGGAGAA 
CGCCCTTATA AATGTGAACT GTGTCCTTAC TCAAGTTCTC AGAAGACTCA TCTAACTCGA 
CACATGCGTA CTCACTCAGG TGAGAAGCCA TTTAAATGTG ATCAGTGCAA TTATGTGGCC 
TCTAATCAGC ACGAAGTGAC CCGACACGCA AGACAGGTTC ACAACGGGCC TAAACCTCTT 
AATTGCCCTC ACTGTGACTA CAAAACAGCC GATAGGAGCA ACTTCAAGAA GCACGTCGAG 
CTGCACGTCA ACCCTCGGCA GTTCAACTGC CCCGTGTGTG ACTACGCGGC CTCCAAGAAG 
TGTAACCTGC AGTACCATTT CAAGTCCAAG CACCCCACCT GCCCCAGCAA GACGATGGAC 
GTGTCCAAAG TGAAGCTGAA GAAAACCAAG AGGAGGGAGG CTGACCTGCA CCGTGACGCC 
GCCGCCGCCG CCACTGAGCA GACGGACACA GAGCAAGCGA AAACCAAGGG GGTGGACGCG 
TCTGCGAGGA GAAGTGAGAG GCCTGTAAAA GGCGTTGGAA AAGATGTTCC AAAAGAGAAG 
AAGCCCTGTA GCAATGCCTC TGTGGTGCAG GTAACTACCC GAACTCGGAA ATCAGCGGTG 
GAGACTAAAG CAGCGGAGGG AAAACACACA GATGGACAGA CAGGAAACAA CGCAGAAAAG 
TCCTCTAAAG CTAAGAAGAG CAAAAGGAAG ATGGACGCCG AGGCCCATCC CTCGGCTGAA 
CCTGTGACTG AGGGACCCGT GACAAAGAAG AAAAAGACGG AGAGCAAACC CAAGACCAGC 
GGCGAAGTGC CGAAGGGCAG CAGAGTGGAG GACAGGAAGG CGGACAAACA GCAAAGTGCT 
TCCATTAAGA AAGGCGGGAA GAAGACGGCT CTCAAGACTA AGACAGCTAA AAAAGGCAGC 
AAACTTGCTC CTAAGGGGGT GGGGCACACA GAACCTTCCT CGGAGATGGC TCAAGGAGGG 
GAGTCTCCAG TTCCTGCTCT CACTCAGGCG GTGGTCACCC CATCAGGATC TACTCAGACA 
GAGCTCTCTT CTCCCATGGA TATTGCTCAG ACAGAGCCTG CCCAGATGGA CGTTTCCCAG 
ACAGGGCCGC CTCAGGTGCA GCGGCCTCTT CCTGTGGAGC CTGCTCAATT GGAGCCGTCT 
CCTCCTCAGG AGCCTCCCCA GGTAGAGCCA CCTGCCTGTG TGGAGCCTCC CCCTCCCGTG 
GAGCCTCCAT GTCCCATGGA GCCTGCTGAG ATGGAACCGT CCCCTCCCAT GGAGCCTTCC 
CAGGTGGAGC CACCTCCTCA TTTGGAGCCT CCGCTTCCCA TGGAGCTGCC TCAGGTGGAG 
CTGCCTCCTG TGGAGGATTG TCAGAAGGAG CTGCCTTCTG TGGAGCATGC TCAGACTAAG 
GTTGCTCAGA CAGGTCCTAC TCAGGTGGGA GCTGTTCAGG AGGAGCCCCT TTCTGTCTCC 
GAGCCACCTC AAGTCAAGCT AACCAGAAGG TCATCTCCCC GGAAAGACCG TGCCAAGGAG 
AAGTTGAGCG TGCTGAGTGA GATGGCGAGG CAGGAGCAGG TTCTTATTGA GGTTGGCTTA 
GTGCCTGTCA GAGATAGCCA GCTTCTGAAG GCCAGCAAGA GCGCACCGGA CCTCCCAGCC 
CCACCGTCAC CACTGCCAAA GGGACACTTG AGAAGAGAAG AGACACCCAA GGACCAAGAA 
ATGTTCTCTG ACGGGGAAGG AAATAAAGTA TCCCCTCTCG AGAAAGGAGG AACAGAGGAA 
GCTGGTGAGA GTCGAGCTGA GCTGGCTGCT CCCATGGAAT CTACCAGTGC TTTATCCTCT 
GAACAAAGCT CAAATGCACC AGATGGTGAA ACATTACACA GCGAGTGTCA GGCTGACTCC 
ACTGCGGTTT GTGAAATGGA AGTGGACACT GAGCAGAAGA CAGACCGTGT CCCTCTGAAA 
GACTCAGCAG TAGAACCAGT GTCACCTCTT AACCCAAGAG TGGACCCTGA AGCAGCGGCA 
CCAGCTGTGG TGGCCTCCCC TCCTATCACT TTGGCCGAGT CTCAGGAAAT TGATGAGGAT 
GAAGGCATTC ACAGCCATGA TGGAAGTGAC CTGAGCGACA ACATGTCTGA GGGGAGTGAT 
GACTCAGGAC TGCATGGGGC TCGGCCAGCA CCACAGGAAG CTACGTCAAA AAGTGGAAAG 
GAAGGGTTGG CTGTCAAAGT AACTGAGGGA GAGTTTGTTT GTATTTTTTG TGATCGTTCT 
TTTAGAAAGG AAAAAGACTA TAGCAAACAC CTCAATCGCC ATTTGGTGAA TGTGTACTTC 
CTTGAAGAAG CAGCTGAGGA GCAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of the Astroglial Glutamate Exchanger xCT in Ventral Hippocampus in Resilience to Stress.
Neuron96(2)402-413.e5(2017 Oct)
Nasca C,Bigio B,Zelli D,de Angelis P,Lau T,Okamoto M,Soya H,Ni J,Brichta L,Greengard P,Neve RL,Lee FS,McEwen BS

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Rest cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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