Stx4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Stx4
Entrez Gene ID	81803
Full Name	syntaxin 4
Synonyms	Stx4a
General protein information	Preferred Names syntaxin 4 Names syntaxin-4 syntaxin 4A (placental)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q37
Summary	involved in docking of synaptic vesicles at presynaptic active zones [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031125.1	NP_112387.1	syntaxin-4
XM_006230348.3	XP_006230410.1	syntaxin-4 isoform X3
XM_006230345.3	XP_006230407.1	syntaxin-4 isoform X1
XM_006230346.3	XP_006230408.1	syntaxin-4 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	STX4	NP_004595.2
Canis lupus familiaris (dog)	STX4	XP_547039.2
Danio rerio (zebrafish)	stx4	NP_956515.1
Pan troglodytes (chimpanzee)	STX4	XP_003315122.1
Bos taurus (cattle)	STX4	NP_001030236.1
	LOC704880	XP_001095454.1
Mus musculus (house mouse)	Stx4a	NP_033320.1
Rattus norvegicus (Norway rat)	Stx4	NP_112387.1

Gene Ontology
Bibliography

Related articles in PubMed

Syntaxin-4 and SNAP23 act as exocytic SNAREs to release NGF from cultured Schwann cells.
Lin M, Jiang M, Ding F, Cao Z
Neuroscience letters65397-104(2017 Jul)

Identification of the SNARE complex mediating the exocytosis of NMDA receptors.
Gu Y, Huganir RL
Proceedings of the National Academy of Sciences of the United States of America113(43)12280-12285(2016 Oct)

CRACM3 regulates the stability of non-excitable exocytotic vesicle fusion pores in a Ca(2+)-independent manner via molecular interaction with syntaxin4.
Liu S, Sahid MN, Takemasa E, Kiyoi T, Kuno M, Oshima Y, Maeyama K
Scientific reports628133(2016 Jun)

The SNARE Protein Syntaxin 3 Confers Specificity for Polarized Axonal Trafficking in Neurons.
Soo Hoo L, Banna CD, Radeke CM, Sharma N, Albertolle ME, Low SH, Weimbs T, Vandenberg CA
PloS one11(9)e0163671(2016)

Transcriptional expression of myelin basic protein in oligodendrocytes depends on functional syntaxin 4: a potential correlation with autocrine signaling.
Bijlard M, Klunder B, de Jonge JC, Nomden A, Tyagi S, de Vries H, Hoekstra D, Baron W
Molecular and cellular biology35(4)675-87(2015 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Transcriptional expression of myelin basic protein in oligodendrocytes depends on functional syntaxin 4.
Title: Transcriptional expression of myelin basic protein in oligodendrocytes depends on functional syntaxin 4: a potential correlation with autocrine signaling.

Syntaxin 1 was localized to the plasma membrane of the presynaptic terminal, including the active zone, while syntaxin 4 was localized to peri-synaptic astroglial processes. Results show that syntaxin 4 is available to support exocytosis in astroglia.
Title: Syntaxin 4 is concentrated on plasma membrane of astrocytes.

These data suggest that Rab17 mediates the dendritic trafficking of Syntaxin-4 to selectively regulate dendritic surface insertion of GluK2-containing KARs in rat hippocampal neurons.
Title: Small GTPase Rab17 regulates the surface expression of kainate receptors but not ?-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in hippocampal neurons via dendritic trafficking of Syntaxin-4 protein.

regulation of mucosal mast cell activation
Title: Regulation of mucosal mast cell activation by short interfering RNAs targeting syntaxin4.

Data showv that complexin 2 interacts with vesicle-associated membrane protein (VAMP) 2, syntaxins 3 and 4.
Title: Complexin 2 modulates vesicle-associated membrane protein (VAMP) 2-regulated zymogen granule exocytosis in pancreatic acini.

The following Stx4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stx4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07677	NM_031125.1 Latest version!	Rattus norvegicus syntaxin 4 (Stx4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa23487	XM_006230345.3 Latest version!	Rattus norvegicus syntaxin 4 (Stx4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa23488	XM_006230346.3 Latest version!	Rattus norvegicus syntaxin 4 (Stx4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa23490	XM_006230348.3 Latest version!	Rattus norvegicus syntaxin 4 (Stx4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Stx4 ( NM_031125.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112387.1 Rattus norvegicus syntaxin 4 (Stx4), mRNA.

Price & Availability↑

CloneID	ORa07677
Clone ID Related Accession (Same CDS sequence)	NM_031125.1
Accession Version	NM_031125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	syntaxin-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L20821.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L20821.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCGCGACA GGACCCATGA GTTGAGGCAG GGGGATAACA TCTCAGACGA TGAAGATGAG 
GTTCGAGTCG CGTTGGTGGT GCACTCAGGT GCCGCCCGGC TGAGCAGCCC GGACGACGAG 
TTCTTCCAGA AGGTGCAGAC AATTCGGCAG ACTATGGCCA AACTGGAGAG TAAAGTCCGG 
GAGTTGGAGA AACAGCAGGT CACCATTCTG GCCACGCCTC TTCCCGAGGA GAGCATGAAG 
CAGGGCCTGC AGAACCTGCG AGAGGAGATC AAACAGCTGG GGAGAGAAGT CCGGGCACAG 
CTAAAAGCCA TAGAGCCCCA GAAGGAAGAA GCTGATGAGA ATTATAATTC AGTCAACACA 
AGAATGAAGA AAACCCAGCA TGGGGTCCTG TCCCAGCAGT TTGTGGAGCT CATCAACAAG 
TGTAACTCAA TGCAGTCCGA GTACCGAGAG AAGAACGTGG AGCGCATTCG GCGGCAGCTG 
AAGATCACCA ATGCTGGAAT GGTGTCTGAC GAGGAACTGG AGCAGATGCT GGACAGTGGG 
CAGAGTGAGG TGTTTGTGTC TAATATACTG AAGGACACAC AGGTGACCCG GCAGGCCCTG 
AATGAGATCT CTGCGCGACA CAGTGAGATC CAGCAGTTGG AGCGCACGAT CCGTGAACTC 
CATGAGATCT TCACTTTTCT AGCTACCGAG GTGGAGATGC AGGGAGAGAT GATCAATCGT 
ATCGAAAAGA ACATTCTGAG CTCAGCAGAC TATGTGGAAC GTGGGCAAGA ACATGTCAAG 
ATAGCGCTAG AGAATCAGAA GAAGGCGAGG AAGAAAAAGG TCATGATTGC CATCTGTGTT 
TCTGTCACTG TTCTCATCTT GGCTGTCATC ATTGGCATCA CCATAACCGT TGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112387.1
CDS	32..928
Translation	MRDRTHELRQGDNISDDEDEVRVALVVHSGAARLSSPDDEFFQKVQTIRQTMAKLESKVRELEKQQVTILATPLPEESMKQGLQNLREEIKQLGREVRAQLKAIEPQKEEADENYNSVNTRMKKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERTIRELHEIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKIALENQKKARKKKVMIAICVSVTVLILAVIIGITITVG

Target ORF information:

RefSeq Version	NM_031125.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus syntaxin 4 (Stx4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031125.1

ORF Insert Sequence:

ATGCGCGACA GGACCCATGA GTTGAGGCAG GGGGATAACA TCTCAGACGA TGAAGATGAG 
GTTCGAGTCG CGTTGGTGGT GCACTCAGGT GCCGCCCGGC TGAGCAGCCC GGACGACGAG 
TTCTTCCAGA AGGTGCAGAC AATTCGGCAG ACTATGGCCA AACTGGAGAG TAAAGTCCGG 
GAGTTGGAGA AACAGCAGGT CACCATTCTG GCCACGCCTC TTCCCGAGGA GAGCATGAAG 
CAGGGCCTGC AGAACCTGCG AGAGGAGATC AAACAGCTGG GGAGAGAAGT CCGGGCACAG 
CTAAAAGCCA TAGAGCCCCA GAAGGAAGAA GCTGATGAGA ATTATAATTC AGTCAACACA 
AGAATGAAGA AAACCCAGCA TGGGGTCCTG TCCCAGCAGT TTGTGGAGCT CATCAACAAG 
TGTAACTCAA TGCAGTCCGA GTACCGAGAG AAGAACGTGG AGCGCATTCG GCGGCAGCTG 
AAGATCACCA ATGCTGGAAT GGTGTCTGAC GAGGAACTGG AGCAGATGCT GGACAGTGGG 
CAGAGTGAGG TGTTTGTGTC TAATATACTG AAGGACACAC AGGTGACCCG GCAGGCCCTG 
AATGAGATCT CTGCGCGACA CAGTGAGATC CAGCAGTTGG AGCGCACGAT CCGTGAACTC 
CATGAGATCT TCACTTTTCT AGCTACCGAG GTGGAGATGC AGGGAGAGAT GATCAATCGT 
ATCGAAAAGA ACATTCTGAG CTCAGCAGAC TATGTGGAAC GTGGGCAAGA ACATGTCAAG 
ATAGCGCTAG AGAATCAGAA GAAGGCGAGG AAGAAAAAGG TCATGATTGC CATCTGTGTT 
TCTGTCACTG TTCTCATCTT GGCTGTCATC ATTGGCATCA CCATAACCGT TGGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stx4 ( XM_006230345.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230407.1 Rattus norvegicus syntaxin 4 (Stx4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23487
Clone ID Related Accession (Same CDS sequence)	XM_006230345.3
Accession Version	XM_006230345.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	syntaxin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230345.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGACA GGACCCATGA GTTGAGGCAG GGGGATAACA TCTCAGACGA TGAAGATGAG 
GTTCGAGTCG CGTTGGTGGT GCACTCAGGT GCCGCCCGGC TGAGCAGCCC GGACGACGAG 
TTCTTCCAGA AGGTGCAGAC AATTCGGCAG ACTATGGCCA AACTGGAGAG TAAAGTCCGG 
GAGTTGGAGA AACAGCAGGT CACCATTCTG GCCACGCCTC TTCCCGAGGA GAGCATGAAG 
CAGGGCCTGC AGAACCTGCG AGAGGAGATC AAACAGCTGG GGAGAGAAGT CCGGGCACAG 
CTAAAAGCCA TAGAGCCCCA GAAGGAAGAA GCTGATGAGA ATTATAATTC AGTCAACACA 
AGAATGAAGA AAACCCAGCA TGGGGTCCTG TCCCAGCAGT TTGTGGAGCT CATCAACAAG 
TGTAACTCAA TGCAGTCCGA GTACCGAGAG AAGAACGTGG AGCGCATTCG GCGGCAGCTG 
AAGATCACCA ATGCTGGAAT GGTGTCTGAC GAGGAACTGG AGCAGATGCT GGACAGTGGG 
CAGAGTGAGG TGTTTGTGTC TAATATACTG AAGGACACAC AGGTGACCCG GCAGGCCCTG 
AATGAGATCT CTGCGCGACA CAGTGAGATC CAGCAGTTGG AGCGCAGCAT CCGTGAACTC 
CATGAGATCT TCACTTTTCT AGCTACCGAG GTGGAGATGC AGGGAGAGAT GATCAATCGT 
ATCGAAAAGA ACATTCTGAG CTCAGCAGAC TATGTGGAAC GTGGGCAAGA ACATGTCAAG 
ATAGCGCTAG AGAATCAGAA GAAGGCGAGG AAGGTAAGCC TGGCCTCTGA CCCCAGCCTT 
GGGCACGCCA GGGTGACTTT CCATGACCCT ACCTCTCCCA CAGAAAAAGG TCATGATTGC 
CATCTGTGTT TCTGTCACTG TTCTCATCTT GGCTGTCATC ATTGGCATCA CCATAACCGT 
TGGATAATGT CACATGTTCT TGGTGAGATA TGGCGGGCCT GCCCCATCCC TGGCAACAGC 
CTTCCCACCT CCTGTCAGAC CCATTTCTTT CTCCTTCCCT TGCAGGCACT GGGAGAAACA 
GAGACCCAGC ATTTGTGGAG GCAGGGCAGG CCTCCAGCTG GACCCACCCT CACACTGGCC 
CTTGTGCAGA AGGGCATATG GCTCTTCTGG GTTGGCAGCT GCTCGTTCAT GGAGTCCTTC 
CCTTCAGGCC ACAATGCCTG GGAGAAAGTT TCCAATGTCC TGTTTGTCTG GGACATATGG 
TTTTATAAAA AATTAAAAAA ACAAAAAAAC AAAAGACCAG AAGCTTGGAG CCAGCTGTGC 
TTGTGTGTTC CAGGAAAGAC TGGCCTAGGC CTCCAAGTAT GCATTCTCCT TTCCCGTGGC 
CATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230407.1
CDS	539..1927
Translation	MRDRTHELRQGDNISDDEDEVRVALVVHSGAARLSSPDDEFFQKVQTIRQTMAKLESKVRELEKQQVTILATPLPEESMKQGLQNLREEIKQLGREVRAQLKAIEPQKEEADENYNSVNTRMKKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHEIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKIALENQKKARKVSLASDPSLGHARVTFHDPTSPTEKGHDCHLCFCHCSHLGCHHWHHHNRWIMSHVLGEIWRACPIPGNSLPTSCQTHFFLLPLQALGETETQHLWRQGRPPAGPTLTLALVQKGIWLFWVGSCSFMESFPSGHNAWEKVSNVLFVWDIWFYKKLKKQKNKRPEAWSQLCLCVPGKTGLGLQVCILLSRGHC

Target ORF information:

RefSeq Version	XM_006230345.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus syntaxin 4 (Stx4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230345.3

ORF Insert Sequence:

ATGCGCGACA GGACCCATGA GTTGAGGCAG GGGGATAACA TCTCAGACGA TGAAGATGAG 
GTTCGAGTCG CGTTGGTGGT GCACTCAGGT GCCGCCCGGC TGAGCAGCCC GGACGACGAG 
TTCTTCCAGA AGGTGCAGAC AATTCGGCAG ACTATGGCCA AACTGGAGAG TAAAGTCCGG 
GAGTTGGAGA AACAGCAGGT CACCATTCTG GCCACGCCTC TTCCCGAGGA GAGCATGAAG 
CAGGGCCTGC AGAACCTGCG AGAGGAGATC AAACAGCTGG GGAGAGAAGT CCGGGCACAG 
CTAAAAGCCA TAGAGCCCCA GAAGGAAGAA GCTGATGAGA ATTATAATTC AGTCAACACA 
AGAATGAAGA AAACCCAGCA TGGGGTCCTG TCCCAGCAGT TTGTGGAGCT CATCAACAAG 
TGTAACTCAA TGCAGTCCGA GTACCGAGAG AAGAACGTGG AGCGCATTCG GCGGCAGCTG 
AAGATCACCA ATGCTGGAAT GGTGTCTGAC GAGGAACTGG AGCAGATGCT GGACAGTGGG 
CAGAGTGAGG TGTTTGTGTC TAATATACTG AAGGACACAC AGGTGACCCG GCAGGCCCTG 
AATGAGATCT CTGCGCGACA CAGTGAGATC CAGCAGTTGG AGCGCAGCAT CCGTGAACTC 
CATGAGATCT TCACTTTTCT AGCTACCGAG GTGGAGATGC AGGGAGAGAT GATCAATCGT 
ATCGAAAAGA ACATTCTGAG CTCAGCAGAC TATGTGGAAC GTGGGCAAGA ACATGTCAAG 
ATAGCGCTAG AGAATCAGAA GAAGGCGAGG AAGGTAAGCC TGGCCTCTGA CCCCAGCCTT 
GGGCACGCCA GGGTGACTTT CCATGACCCT ACCTCTCCCA CAGAAAAAGG TCATGATTGC 
CATCTGTGTT TCTGTCACTG TTCTCATCTT GGCTGTCATC ATTGGCATCA CCATAACCGT 
TGGATAATGT CACATGTTCT TGGTGAGATA TGGCGGGCCT GCCCCATCCC TGGCAACAGC 
CTTCCCACCT CCTGTCAGAC CCATTTCTTT CTCCTTCCCT TGCAGGCACT GGGAGAAACA 
GAGACCCAGC ATTTGTGGAG GCAGGGCAGG CCTCCAGCTG GACCCACCCT CACACTGGCC 
CTTGTGCAGA AGGGCATATG GCTCTTCTGG GTTGGCAGCT GCTCGTTCAT GGAGTCCTTC 
CCTTCAGGCC ACAATGCCTG GGAGAAAGTT TCCAATGTCC TGTTTGTCTG GGACATATGG 
TTTTATAAAA AATTAAAAAA ACAAAAAAAC AAAAGACCAG AAGCTTGGAG CCAGCTGTGC 
TTGTGTGTTC CAGGAAAGAC TGGCCTAGGC CTCCAAGTAT GCATTCTCCT TTCCCGTGGC 
CATTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stx4 ( XM_006230346.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230408.1 Rattus norvegicus syntaxin 4 (Stx4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa23488
Clone ID Related Accession (Same CDS sequence)	XM_006230346.3
Accession Version	XM_006230346.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	syntaxin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230346.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAAC TGGAGAGTAA AGTCCGGGAG TTGGAGAAAC AGCAGGTCAC CATTCTGGCC 
ACGCCTCTTC CCGAGGAGAG CATGAAGCAG GGCCTGCAGA ACCTGCGAGA GGAGATCAAA 
CAGCTGGGGA GAGAAGTCCG GGCACAGCTA AAAGCCATAG AGCCCCAGAA GGAAGAAGCT 
GATGAGAATT ATAATTCAGT CAACACAAGA ATGAAGAAAA CCCAGCATGG GGTCCTGTCC 
CAGCAGTTTG TGGAGCTCAT CAACAAGTGT AACTCAATGC AGTCCGAGTA CCGAGAGAAG 
AACGTGGAGC GCATTCGGCG GCAGCTGAAG ATCACCAATG CTGGAATGGT GTCTGACGAG 
GAACTGGAGC AGATGCTGGA CAGTGGGCAG AGTGAGGTGT TTGTGTCTAA TATACTGAAG 
GACACACAGG TGACCCGGCA GGCCCTGAAT GAGATCTCTG CGCGACACAG TGAGATCCAG 
CAGTTGGAGC GCAGCATCCG TGAACTCCAT GAGATCTTCA CTTTTCTAGC TACCGAGGTG 
GAGATGCAGG GAGAGATGAT CAATCGTATC GAAAAGAACA TTCTGAGCTC AGCAGACTAT 
GTGGAACGTG GGCAAGAACA TGTCAAGATA GCGCTAGAGA ATCAGAAGAA GGCGAGGAAG 
GTAAGCCTGG CCTCTGACCC CAGCCTTGGG CACGCCAGGG TGACTTTCCA TGACCCTACC 
TCTCCCACAG AAAAAGGTCA TGATTGCCAT CTGTGTTTCT GTCACTGTTC TCATCTTGGC 
TGTCATCATT GGCATCACCA TAACCGTTGG ATAATGTCAC ATGTTCTTGG TGAGATATGG 
CGGGCCTGCC CCATCCCTGG CAACAGCCTT CCCACCTCCT GTCAGACCCA TTTCTTTCTC 
CTTCCCTTGC AGGCACTGGG AGAAACAGAG ACCCAGCATT TGTGGAGGCA GGGCAGGCCT 
CCAGCTGGAC CCACCCTCAC ACTGGCCCTT GTGCAGAAGG GCATATGGCT CTTCTGGGTT 
GGCAGCTGCT CGTTCATGGA GTCCTTCCCT TCAGGCCACA ATGCCTGGGA GAAAGTTTCC 
AATGTCCTGT TTGTCTGGGA CATATGGTTT TATAAAAAAT TAAAAAAACA AAAAAACAAA 
AGACCAGAAG CTTGGAGCCA GCTGTGCTTG TGTGTTCCAG GAAAGACTGG CCTAGGCCTC 
CAAGTATGCA TTCTCCTTTC CCGTGGCCAT TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230408.1
CDS	719..1954
Translation	MAKLESKVRELEKQQVTILATPLPEESMKQGLQNLREEIKQLGREVRAQLKAIEPQKEEADENYNSVNTRMKKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHEIFTFLATEVEMQGEMINRIEKNILSSADYVERGQEHVKIALENQKKARKVSLASDPSLGHARVTFHDPTSPTEKGHDCHLCFCHCSHLGCHHWHHHNRWIMSHVLGEIWRACPIPGNSLPTSCQTHFFLLPLQALGETETQHLWRQGRPPAGPTLTLALVQKGIWLFWVGSCSFMESFPSGHNAWEKVSNVLFVWDIWFYKKLKKQKNKRPEAWSQLCLCVPGKTGLGLQVCILLSRGHC

Target ORF information:

RefSeq Version	XM_006230346.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus syntaxin 4 (Stx4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230346.3

ORF Insert Sequence:

ATGGCCAAAC TGGAGAGTAA AGTCCGGGAG TTGGAGAAAC AGCAGGTCAC CATTCTGGCC 
ACGCCTCTTC CCGAGGAGAG CATGAAGCAG GGCCTGCAGA ACCTGCGAGA GGAGATCAAA 
CAGCTGGGGA GAGAAGTCCG GGCACAGCTA AAAGCCATAG AGCCCCAGAA GGAAGAAGCT 
GATGAGAATT ATAATTCAGT CAACACAAGA ATGAAGAAAA CCCAGCATGG GGTCCTGTCC 
CAGCAGTTTG TGGAGCTCAT CAACAAGTGT AACTCAATGC AGTCCGAGTA CCGAGAGAAG 
AACGTGGAGC GCATTCGGCG GCAGCTGAAG ATCACCAATG CTGGAATGGT GTCTGACGAG 
GAACTGGAGC AGATGCTGGA CAGTGGGCAG AGTGAGGTGT TTGTGTCTAA TATACTGAAG 
GACACACAGG TGACCCGGCA GGCCCTGAAT GAGATCTCTG CGCGACACAG TGAGATCCAG 
CAGTTGGAGC GCAGCATCCG TGAACTCCAT GAGATCTTCA CTTTTCTAGC TACCGAGGTG 
GAGATGCAGG GAGAGATGAT CAATCGTATC GAAAAGAACA TTCTGAGCTC AGCAGACTAT 
GTGGAACGTG GGCAAGAACA TGTCAAGATA GCGCTAGAGA ATCAGAAGAA GGCGAGGAAG 
GTAAGCCTGG CCTCTGACCC CAGCCTTGGG CACGCCAGGG TGACTTTCCA TGACCCTACC 
TCTCCCACAG AAAAAGGTCA TGATTGCCAT CTGTGTTTCT GTCACTGTTC TCATCTTGGC 
TGTCATCATT GGCATCACCA TAACCGTTGG ATAATGTCAC ATGTTCTTGG TGAGATATGG 
CGGGCCTGCC CCATCCCTGG CAACAGCCTT CCCACCTCCT GTCAGACCCA TTTCTTTCTC 
CTTCCCTTGC AGGCACTGGG AGAAACAGAG ACCCAGCATT TGTGGAGGCA GGGCAGGCCT 
CCAGCTGGAC CCACCCTCAC ACTGGCCCTT GTGCAGAAGG GCATATGGCT CTTCTGGGTT 
GGCAGCTGCT CGTTCATGGA GTCCTTCCCT TCAGGCCACA ATGCCTGGGA GAAAGTTTCC 
AATGTCCTGT TTGTCTGGGA CATATGGTTT TATAAAAAAT TAAAAAAACA AAAAAACAAA 
AGACCAGAAG CTTGGAGCCA GCTGTGCTTG TGTGTTCCAG GAAAGACTGG CCTAGGCCTC 
CAAGTATGCA TTCTCCTTTC CCGTGGCCAT TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Stx4 ( XM_006230348.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230410.1 Rattus norvegicus syntaxin 4 (Stx4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa23490
Clone ID Related Accession (Same CDS sequence)	XM_006230348.3
Accession Version	XM_006230348.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	syntaxin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230348.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCGACA GGACCCATGA GTTGAGGCAG GGGGATAACA TCTCAGACGA TGAAGATGAG 
GTTCGAGTCG CGTTGGTGGT GCACTCAGGT GCCGCCCGGC TGAGCAGCCC GGACGACGAG 
TTCTTCCAGA AGGTGCAGAC AATTCGGCAG ACTATGGCCA AACTGGAGAG TAAAGTCCGG 
GAGTTGGAGA AACAGCAGGT CACCATTCTG GCCACGCCTC TTCCCGAGGA GAGCATGAAG 
CAGGGCCTGC AGAACCTGCG AGAGGAGATC AAACAGCTGG GGAGAGAAGT CCGGGCACAG 
CTAAAAGCCA TAGAGCCCCA GAAGGAAGAA GCTGATGAGA ATTATAATTC AGTCAACACA 
AGAATGAAGA AAACCCAGCA TGGGGTCCTG TCCCAGCAGT TTGTGGAGCT CATCAACAAG 
TGTAACTCAA TGCAGTCCGA GTACCGAGAG AAGAACGTGG AGCGCATTCG GCGGCAGCTG 
AAGATCACCA ATGCTGGAAT GGTGTCTGAC GAGGAACTGG AGCAGATGCT GGACAGTGGG 
CAGAGTGAGG TGTTTGTGTC TAATATACTG AAGGACACAC AGGTGACCCG GCAGGCCCTG 
AATGAGATCT CTGCGCGACA CAGTGAGATC CAGCAGTTGG AGCGCAGCAT CCGTGAACTC 
CATGAGATCT TCACTTTTCT AGCTACCGAG GTGGAGATGC AGGTTAGAAC GGCCCTGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230410.1
CDS	534..1256
Translation	MRDRTHELRQGDNISDDEDEVRVALVVHSGAARLSSPDDEFFQKVQTIRQTMAKLESKVRELEKQQVTILATPLPEESMKQGLQNLREEIKQLGREVRAQLKAIEPQKEEADENYNSVNTRMKKTQHGVLSQQFVELINKCNSMQSEYREKNVERIRRQLKITNAGMVSDEELEQMLDSGQSEVFVSNILKDTQVTRQALNEISARHSEIQQLERSIRELHEIFTFLATEVEMQVRTALS

Target ORF information:

RefSeq Version	XM_006230348.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus syntaxin 4 (Stx4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230348.3

ORF Insert Sequence:

ATGCGCGACA GGACCCATGA GTTGAGGCAG GGGGATAACA TCTCAGACGA TGAAGATGAG 
GTTCGAGTCG CGTTGGTGGT GCACTCAGGT GCCGCCCGGC TGAGCAGCCC GGACGACGAG 
TTCTTCCAGA AGGTGCAGAC AATTCGGCAG ACTATGGCCA AACTGGAGAG TAAAGTCCGG 
GAGTTGGAGA AACAGCAGGT CACCATTCTG GCCACGCCTC TTCCCGAGGA GAGCATGAAG 
CAGGGCCTGC AGAACCTGCG AGAGGAGATC AAACAGCTGG GGAGAGAAGT CCGGGCACAG 
CTAAAAGCCA TAGAGCCCCA GAAGGAAGAA GCTGATGAGA ATTATAATTC AGTCAACACA 
AGAATGAAGA AAACCCAGCA TGGGGTCCTG TCCCAGCAGT TTGTGGAGCT CATCAACAAG 
TGTAACTCAA TGCAGTCCGA GTACCGAGAG AAGAACGTGG AGCGCATTCG GCGGCAGCTG 
AAGATCACCA ATGCTGGAAT GGTGTCTGAC GAGGAACTGG AGCAGATGCT GGACAGTGGG 
CAGAGTGAGG TGTTTGTGTC TAATATACTG AAGGACACAC AGGTGACCCG GCAGGCCCTG 
AATGAGATCT CTGCGCGACA CAGTGAGATC CAGCAGTTGG AGCGCAGCAT CCGTGAACTC 
CATGAGATCT TCACTTTTCT AGCTACCGAG GTGGAGATGC AGGTTAGAAC GGCCCTGTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Syntaxin-4 and SNAP23 act as exocytic SNAREs to release NGF from cultured Schwann cells.
Neuroscience letters65397-104(2017 Jul)
Lin M,Jiang M,Ding F,Cao Z

Identification of the SNARE complex mediating the exocytosis of NMDA receptors.
Proceedings of the National Academy of Sciences of the United States of America113(43)12280-12285(2016 Oct)
Gu Y,Huganir RL

CRACM3 regulates the stability of non-excitable exocytotic vesicle fusion pores in a Ca(2+)-independent manner via molecular interaction with syntaxin4.
Scientific reports628133(2016 Jun)
Liu S,Sahid MN,Takemasa E,Kiyoi T,Kuno M,Oshima Y,Maeyama K

The SNARE Protein Syntaxin 3 Confers Specificity for Polarized Axonal Trafficking in Neurons.
PloS one11(9)e0163671(2016)
Soo Hoo L,Banna CD,Radeke CM,Sharma N,Albertolle ME,Low SH,Weimbs T,Vandenberg CA

Transcriptional expression of myelin basic protein in oligodendrocytes depends on functional syntaxin 4: a potential correlation with autocrine signaling.
Molecular and cellular biology35(4)675-87(2015 Feb)
Bijlard M,Klunder B,de Jonge JC,Nomden A,Tyagi S,de Vries H,Hoekstra D,Baron W

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Stx4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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