Pggt1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pggt1b
Entrez Gene ID	81746
Full Name	protein geranylgeranyltransferase type 1 subunit beta
General protein information	Preferred Names protein geranylgeranyltransferase type 1 subunit beta Names geranylgeranyl transferase type-1 subunit beta GGTase-I-beta geranylgeranyl transferase type I subunit beta geranylgeranyltransferase type 1 beta geranylgeranyltransferase type I (GGTase-I) protein geranylgeranyltransferase type I, beta subunit type I protein geranyl-geranyltransferase subunit beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q11
Summary	catalyzes the transfer of a geranylgeranyl group to the cysteine residue of proteins containing a carboxyl-terminal CAAX where X is preferably leucine [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008772177.2	XP_008770399.1	geranylgeranyl transferase type-1 subunit beta isoform X1
NM_031082.1	NP_112344.1	geranylgeranyl transferase type-1 subunit beta

Pathways from BioSystems

Homologies

Homo sapiens (human)	PGGT1B	NP_005014.2
Mus musculus (house mouse)	Pggt1b	NP_766215.1
Gallus gallus (chicken)	PGGT1B	XP_003643150.1
Arabidopsis thaliana (thale cress)	PGGT-I	NP_181487.1
Xenopus tropicalis (tropical clawed frog)	pggt1b	NP_001008137.1
Pan troglodytes (chimpanzee)	PGGT1B	XP_526978.2
Canis lupus familiaris (dog)	PGGT1B	XP_538560.2
Rattus norvegicus (Norway rat)	Pggt1b	NP_112344.1
Drosophila melanogaster (fruit fly)	betaggt-I	NP_001260061.1
Bos taurus (cattle)	PGGT1B	NP_001015560.1
Oryza sativa (rice)	Os01g0150100	NP_001042031.1
	LOC696437	XP_002808482.1
Danio rerio (zebrafish)	pggt1b	NP_001025426.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008406	XP_317045.4
Caenorhabditis elegans (roundworm)	Y48E1B.3	NP_001254397.1

Gene Ontology
Bibliography

Related articles in PubMed

Geranylgeranyltransferase I is essential for dendritic development of cerebellar Purkinje cells.
Wu KY, Zhou XP, Luo ZG
Molecular brain318(2010 Jun)

TrkB-mediated activation of geranylgeranyltransferase I promotes dendritic morphogenesis.
Zhou XP, Wu KY, Liang B, Fu XQ, Luo ZG
Proceedings of the National Academy of Sciences of the United States of America105(44)17181-6(2008 Nov)

Conversion of protein farnesyltransferase to a geranylgeranyltransferase.
Terry KL, Casey PJ, Beese LS
Biochemistry45(32)9746-55(2006 Aug)

Crystallographic analysis of CaaX prenyltransferases complexed with substrates defines rules of protein substrate selectivity.
Reid TS, Terry KL, Casey PJ, Beese LS
Journal of molecular biology343(2)417-33(2004 Oct)

Crystallographic analysis reveals that anticancer clinical candidate L-778,123 inhibits protein farnesyltransferase and geranylgeranyltransferase-I by different binding modes.
Reid TS, Long SB, Beese LS
Biochemistry43(28)9000-8(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results indicate that geranylgeranyltransferase I (GGT) plays an important role in Purkinje cell development, and suggest a novel role of GGT in neuronal morphogenesis in vivo.
Title: Geranylgeranyltransferase I is essential for dendritic development of cerebellar Purkinje cells.

lysine beta311 of GGTase I partially replaces the catalytic function of Mg(II) observed in FTas
Title: Lysine beta311 of protein geranylgeranyltransferase type I partially replaces magnesium.

The following Pggt1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pggt1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07542	NM_031082.1 Latest version!	Rattus norvegicus protein geranylgeranyltransferase type I subunit beta (Pggt1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa38368	XM_008772177.2 Latest version!	Rattus norvegicus protein geranylgeranyltransferase type 1 subunit beta (Pggt1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pggt1b ( NM_031082.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112344.1 Rattus norvegicus protein geranylgeranyltransferase type I subunit beta (Pggt1b), mRNA.

Price & Availability↑

CloneID	ORa07542
Clone ID Related Accession (Same CDS sequence)	NM_031082.1
Accession Version	NM_031082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Rattus norvegicus(Norway rat)
Product	geranylgeranyl transferase type-1 subunit beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L24116.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L24116.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCA CAGAGGATGA CAGACTGGCG GGGAGCGGAG AAGGAGAACG GCTGGATTTC 
CTGCGGGACC GACACGTGCG GTTCTTCCAG CGCTGCCTCC AGGTCTTGCC GGAGCGGTAT 
TCTTCGCTGG AGACCAGCAG GCTGACAATT GCATTTTTTG CACTCTCTGG GCTGGATATG 
TTGGACTCCT TGGATGTGGT GAACAAAGAC GATATAATAG AGTGGATTTA TTCCTTGCAG 
GTTCTTCCCA CAGAAGACAG GTCAAATCTG GATCGCTGTG GTTTCCGAGG TTCTTCATAT 
TTGGGTATTC CATTCAACCC ATCAAAGAAT CCAGGCACAG CTCATCCTTA TGACAGTGGA 
CACATAGCGA TGACTTACAC TGGTCTTTCC TGTTTAATTA TTCTTGGAGA TGATTTAAGC 
CGAGTAGATA AAGAAGCTTG CTTAGCAGGC TTGAGAGCAC TTCAGCTGGA AGATGGGAGC 
TTCTGTGCTG TTCCTGAAGG CAGTGAGAAT GACATGAGGT TTGTGTACTG TGCTTCCTGC 
ATTTGCTATA TGCTCAACAA CTGGTCAGGC ATGGATATGA AGAAAGCCAT CAGCTACATT 
AGAAGAAGTA TGTCCTATGA CAATGGCCTG GCACAGGGGG CAGGACTTGA GTCTCATGGA 
GGATCCACCT TTTGTGGCAT TGCGTCACTG TGCCTGATGG GTAAACTGGA AGAAGTTTTT 
TCAGAGAAAG AACTGAACCG GATAAAGAGG TGGTGCATAA TGAGGCAGCA GAACGGGTAC 
CACGGAAGAC CTAACAAGCC TGTCGACACC TGTTACTCTT TCTGGGTGGG AGCAACACTA 
AAGCTTTTGA AAATTTTCCA GTACACTAAC TTTGAGAAGA ATAGGAATTA CATCTTATCA 
ACTCAGGATC GCCTTGTTGG GGGATTTGCT AAATGGCCAG ACAGTCATCC AGATGCTTTG 
CATGCGTACT TCGGGATCTG TGGCCTGTCA CTAATGGAGG AGAGTGGAAT TTGTAAAGTT 
CATCCTGCTC TGAATGTAAG CACACGAACT TCTGAGCGCC TCCGAGATCT CCATCAAAGC 
TGGAAGACCA AGGACTCTAA ACAGTGCTCA GACAATGTCC ATATTTCCAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112344.1
CDS	10..1143
Translation	MAATEDDRLAGSGEGERLDFLRDRHVRFFQRCLQVLPERYSSLETSRLTIAFFALSGLDMLDSLDVVNKDDIIEWIYSLQVLPTEDRSNLDRCGFRGSSYLGIPFNPSKNPGTAHPYDSGHIAMTYTGLSCLIILGDDLSRVDKEACLAGLRALQLEDGSFCAVPEGSENDMRFVYCASCICYMLNNWSGMDMKKAISYIRRSMSYDNGLAQGAGLESHGGSTFCGIASLCLMGKLEEVFSEKELNRIKRWCIMRQQNGYHGRPNKPVDTCYSFWVGATLKLLKIFQYTNFEKNRNYILSTQDRLVGGFAKWPDSHPDALHAYFGICGLSLMEESGICKVHPALNVSTRTSERLRDLHQSWKTKDSKQCSDNVHISS

Target ORF information:

RefSeq Version	NM_031082.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein geranylgeranyltransferase type I subunit beta (Pggt1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031082.1

ORF Insert Sequence:

ATGGCGGCCA CAGAGGATGA CAGACTGGCG GGGAGCGGAG AAGGAGAACG GCTGGATTTC 
CTGCGGGACC GACACGTGCG GTTCTTCCAG CGCTGCCTCC AGGTCTTGCC GGAGCGGTAT 
TCTTCGCTGG AGACCAGCAG GCTGACAATT GCATTTTTTG CACTCTCTGG GCTGGATATG 
TTGGACTCCT TGGATGTGGT GAACAAAGAC GATATAATAG AGTGGATTTA TTCCTTGCAG 
GTTCTTCCCA CAGAAGACAG GTCAAATCTG GATCGCTGTG GTTTCCGAGG TTCTTCATAT 
TTGGGTATTC CATTCAACCC ATCAAAGAAT CCAGGCACAG CTCATCCTTA TGACAGTGGA 
CACATAGCGA TGACTTACAC TGGTCTTTCC TGTTTAATTA TTCTTGGAGA TGATTTAAGC 
CGAGTAGATA AAGAAGCTTG CTTAGCAGGC TTGAGAGCAC TTCAGCTGGA AGATGGGAGC 
TTCTGTGCTG TTCCTGAAGG CAGTGAGAAT GACATGAGGT TTGTGTACTG TGCTTCCTGC 
ATTTGCTATA TGCTCAACAA CTGGTCAGGC ATGGATATGA AGAAAGCCAT CAGCTACATT 
AGAAGAAGTA TGTCCTATGA CAATGGCCTG GCACAGGGGG CAGGACTTGA GTCTCATGGA 
GGATCCACCT TTTGTGGCAT TGCGTCACTG TGCCTGATGG GTAAACTGGA AGAAGTTTTT 
TCAGAGAAAG AACTGAACCG GATAAAGAGG TGGTGCATAA TGAGGCAGCA GAACGGGTAC 
CACGGAAGAC CTAACAAGCC TGTCGACACC TGTTACTCTT TCTGGGTGGG AGCAACACTA 
AAGCTTTTGA AAATTTTCCA GTACACTAAC TTTGAGAAGA ATAGGAATTA CATCTTATCA 
ACTCAGGATC GCCTTGTTGG GGGATTTGCT AAATGGCCAG ACAGTCATCC AGATGCTTTG 
CATGCGTACT TCGGGATCTG TGGCCTGTCA CTAATGGAGG AGAGTGGAAT TTGTAAAGTT 
CATCCTGCTC TGAATGTAAG CACACGAACT TCTGAGCGCC TCCGAGATCT CCATCAAAGC 
TGGAAGACCA AGGACTCTAA ACAGTGCTCA GACAATGTCC ATATTTCCAG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pggt1b ( XM_008772177.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008770399.1 Rattus norvegicus protein geranylgeranyltransferase type 1 subunit beta (Pggt1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38368
Clone ID Related Accession (Same CDS sequence)	XM_008772177.2
Accession Version	XM_008772177.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	geranylgeranyl transferase type-1 subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008772177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACT CCTTGGATGT GGTGAACAAA GACGATATAA TAGAGTGGAT TTATTCCTTG 
CAGGTTCTTC CCACAGAAGA CAGGTCAAAT CTGGATCGCT GTGGTTTCCG AGGTTCTTCA 
TATTTGGGTA TTCCATTCAA CCCATCAAAG AATCCAGGCA CAGCTCATCC TTATGACAGT 
GGACACATAG CGATGACTTA CACTGGTCTT TCCTGTTTAA TTATTCTTGG AGATGATTTA 
AGCCGAGTAG ATAAAGAAGC TTGCTTAGCA GGCTTGAGAG CACTTCAGCT GGAAGATGGG 
AGCTTCTGTG CTGTTCCTGA AGGCAGTGAG AATGACATGA GGTTTGTGTA CTGTGCTTCC 
TGCATTTGCT ATATGCTCAA CAACTGGTCA GGCATGGATA TGAAGAAAGC CATCAGCTAC 
ATTAGAAGAA GTATGTCCTA TGACAATGGC CTGGCACAGG GGGCAGGACT TGAGTCTCAT 
GGAGGATCCA CCTTTTGTGG CATTGCGTCA CTGTGCCTGA TGGGTAAACT GGAAGAAGTT 
TTTTCAGAGA AAGAACTGAA CCGGATAAAG AGGTGGTGCA TAATGAGGCA GCAGAACGGG 
TACCACGGAA GACCTAACAA GCCTGTCGAC ACCTGTTACT CTTTCTGGGT GGGAGCAACA 
CTAAAGCTTT TGAAAATTTT CCAGTACACT AACTTTGAGA AGAATAGGAA TTACATCTTA 
TCAACTCAGG ATCGCCTTGT TGGGGGATTT GCTAAATGGC CAGACAGTCA TCCAGATGCT 
TTGCATGCGT ACTTCGGGAT CTGTGGCCTG TCACTAATGG AGGAGAGTGG AATTTGTAAA 
GTTCATCCTG CTCTGAATGT AAGCACACGA ACTTCTGAGC GCCTCCGAGA TCTCCATCAA 
AGCTGGAAGA CCAAGGACTC TAAACAGTGC TCAGACAATG TCCATATTTC CAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008770399.1
CDS	202..1158
Translation	MLDSLDVVNKDDIIEWIYSLQVLPTEDRSNLDRCGFRGSSYLGIPFNPSKNPGTAHPYDSGHIAMTYTGLSCLIILGDDLSRVDKEACLAGLRALQLEDGSFCAVPEGSENDMRFVYCASCICYMLNNWSGMDMKKAISYIRRSMSYDNGLAQGAGLESHGGSTFCGIASLCLMGKLEEVFSEKELNRIKRWCIMRQQNGYHGRPNKPVDTCYSFWVGATLKLLKIFQYTNFEKNRNYILSTQDRLVGGFAKWPDSHPDALHAYFGICGLSLMEESGICKVHPALNVSTRTSERLRDLHQSWKTKDSKQCSDNVHISS

Target ORF information:

RefSeq Version	XM_008772177.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein geranylgeranyltransferase type 1 subunit beta (Pggt1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008772177.2

ORF Insert Sequence:

ATGTTGGACT CCTTGGATGT GGTGAACAAA GACGATATAA TAGAGTGGAT TTATTCCTTG 
CAGGTTCTTC CCACAGAAGA CAGGTCAAAT CTGGATCGCT GTGGTTTCCG AGGTTCTTCA 
TATTTGGGTA TTCCATTCAA CCCATCAAAG AATCCAGGCA CAGCTCATCC TTATGACAGT 
GGACACATAG CGATGACTTA CACTGGTCTT TCCTGTTTAA TTATTCTTGG AGATGATTTA 
AGCCGAGTAG ATAAAGAAGC TTGCTTAGCA GGCTTGAGAG CACTTCAGCT GGAAGATGGG 
AGCTTCTGTG CTGTTCCTGA AGGCAGTGAG AATGACATGA GGTTTGTGTA CTGTGCTTCC 
TGCATTTGCT ATATGCTCAA CAACTGGTCA GGCATGGATA TGAAGAAAGC CATCAGCTAC 
ATTAGAAGAA GTATGTCCTA TGACAATGGC CTGGCACAGG GGGCAGGACT TGAGTCTCAT 
GGAGGATCCA CCTTTTGTGG CATTGCGTCA CTGTGCCTGA TGGGTAAACT GGAAGAAGTT 
TTTTCAGAGA AAGAACTGAA CCGGATAAAG AGGTGGTGCA TAATGAGGCA GCAGAACGGG 
TACCACGGAA GACCTAACAA GCCTGTCGAC ACCTGTTACT CTTTCTGGGT GGGAGCAACA 
CTAAAGCTTT TGAAAATTTT CCAGTACACT AACTTTGAGA AGAATAGGAA TTACATCTTA 
TCAACTCAGG ATCGCCTTGT TGGGGGATTT GCTAAATGGC CAGACAGTCA TCCAGATGCT 
TTGCATGCGT ACTTCGGGAT CTGTGGCCTG TCACTAATGG AGGAGAGTGG AATTTGTAAA 
GTTCATCCTG CTCTGAATGT AAGCACACGA ACTTCTGAGC GCCTCCGAGA TCTCCATCAA 
AGCTGGAAGA CCAAGGACTC TAAACAGTGC TCAGACAATG TCCATATTTC CAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Geranylgeranyltransferase I is essential for dendritic development of cerebellar Purkinje cells.
Molecular brain318(2010 Jun)
Wu KY,Zhou XP,Luo ZG

TrkB-mediated activation of geranylgeranyltransferase I promotes dendritic morphogenesis.
Proceedings of the National Academy of Sciences of the United States of America105(44)17181-6(2008 Nov)
Zhou XP,Wu KY,Liang B,Fu XQ,Luo ZG

Conversion of protein farnesyltransferase to a geranylgeranyltransferase.
Biochemistry45(32)9746-55(2006 Aug)
Terry KL,Casey PJ,Beese LS

Crystallographic analysis of CaaX prenyltransferases complexed with substrates defines rules of protein substrate selectivity.
Journal of molecular biology343(2)417-33(2004 Oct)
Reid TS,Terry KL,Casey PJ,Beese LS

Crystallographic analysis reveals that anticancer clinical candidate L-778,123 inhibits protein farnesyltransferase and geranylgeranyltransferase-I by different binding modes.
Biochemistry43(28)9000-8(2004 Jul)
Reid TS,Long SB,Beese LS