pggt1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pggt1b
Entrez Gene ID	570808
Full Name	protein geranylgeranyltransferase type I, beta subunit
Synonyms	zgc:114070
General protein information	Preferred Names protein geranylgeranyltransferase type I, beta subunit Names geranylgeranyl transferase type-1 subunit beta ggt1b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001030255.2	NP_001025426.1	geranylgeranyl transferase type-1 subunit beta

Pathways from BioSystems

Homologies

Homo sapiens (human)	PGGT1B	NP_005014.2
Mus musculus (house mouse)	Pggt1b	NP_766215.1
Gallus gallus (chicken)	PGGT1B	XP_003643150.1
Arabidopsis thaliana (thale cress)	PGGT-I	NP_181487.1
Xenopus tropicalis (tropical clawed frog)	pggt1b	NP_001008137.1
Pan troglodytes (chimpanzee)	PGGT1B	XP_526978.2
Canis lupus familiaris (dog)	PGGT1B	XP_538560.2
Rattus norvegicus (Norway rat)	Pggt1b	NP_112344.1
Drosophila melanogaster (fruit fly)	betaggt-I	NP_001260061.1
Bos taurus (cattle)	PGGT1B	NP_001015560.1
Oryza sativa (rice)	Os01g0150100	NP_001042031.1
	LOC696437	XP_002808482.1
Danio rerio (zebrafish)	pggt1b	NP_001025426.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008406	XP_317045.4
Caenorhabditis elegans (roundworm)	Y48E1B.3	NP_001254397.1

Gene Ontology
Bibliography

Related articles in PubMed

Disruption of
El-Rass S, Eisa-Beygi S, Khong E, Brand-Arzamendi K, Mauro A, Zhang H, Clark KJ, Ekker SC, Wen XY
Biology open6(3)348-357(2017 Mar)

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
Sullivan C, Lage CR, Yoder JA, Postlethwait JH, Kim CH
PloS one12(6)e0179517(2017)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

A zebrafish chemical suppressor screening identifies small molecule inhibitors of the Wnt/?-catenin pathway.
Nishiya N, Oku Y, Kumagai Y, Sato Y, Yamaguchi E, Sasaki A, Shoji M, Ohnishi Y, Okamoto H, Uehara Y
Chemistry & biology21(4)530-540(2014 Apr)

The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) pathway regulates developmental cerebral-vascular stability via prenylation-dependent signalling pathway.
Eisa-Beygi S, Hatch G, Noble S, Ekker M, Moon TW
Developmental biology373(2)258-66(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pggt1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pggt1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa30438	NM_001030255.2 Latest version!	Danio rerio protein geranylgeranyltransferase type I, beta subunit (pggt1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pggt1b ( NM_001030255.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001025426.1 Danio rerio protein geranylgeranyltransferase type I, beta subunit (pggt1b), mRNA.

Price & Availability↑

CloneID	ODa30438
Clone ID Related Accession (Same CDS sequence)	NM_001030255.2
Accession Version	NM_001030255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-11
Organism	Danio rerio(zebrafish)
Product	geranylgeranyl transferase type-1 subunit beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC097132.1. On Apr 2, 2013 this sequence version replaced NM_001030255.1. ##Evidence-Data-START## Transcript exon combination :: BC097132.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGATT TTGAGTCGGT GGATTTCCTC CGTGAGCGGC ACGTGCGCTT CTTCCAGCGC 
TGCCTGTACG TGTTACCGGA GAGATATGCG CCGTACGAGA CCAGCCGGCT GACGATAGCC 
TTCTTCTCTC TGTCGGGTCT GGATGTTCTG GGCGCTCTGG ATGTTGTGGA CAGACACAGT 
CTGATCGAGT GGATTTACTC TCTGCAGATC CTGCCCACCG CTGACCAGTC GAATCTCCAG 
CGCTGTGGCT TCAGAGGATC TTCCCACATT GGTGTTCCCT ACAGCTCGTC TAAGGGCCCC 
GGGGCTCCTC ACCCCTATGA CAGTGGGCAC GTGACCATGA CCTACACCGG GCTGGCGTGT 
CTGCTGATCT TGGGTGATGA TCTGGGCCGT GTGGATCGGG CCGCGTGTGT GTCGGGGCTG 
CGTGCGCTGC AGCTGGAGGA CGGCAGCTTC TACGCCGTGC CCGAGGGCAG CGAGAACGAC 
ATGCGCTTCG TCTACTGCGC CGCCTGCATC TGCTTCATGC TGGACGACTG GAGCGGGATG 
GACCGGCAGA AGACCATCGA CTACATCCGC AGGAGCACAT CGTTTGACTT CGGTATCGGG 
CAGGGCGCCG GCCTGGAGTC TCACGGTGGC TCCACCTTCT GTGCCGTGGC GTCGCTGTGC 
ATGATGGGAA AGCTGCGGGA GGTGTTCAGC GAGCGTGAGC TGGGTCGTCT GCGCCGCTGG 
TGTGTGCTCC GACAGCAGAA CGGCTTCCAG GGACGACCCA ACAAACCTGT GGACACCTGC 
TACTCCTTCT GGGTCGGGGC CACGCTGCAG CTGTTGGATG TGTTCCAGTA CACAAACTTT 
GAGAAGAACC GGAACTATAT TCTGTCCACT CAGGACCGGC TGGTGGGCGG CTTCGCTAAA 
TGGCCCGACA GTCACCCAGA CCCTCTCCAC ACCTACTTCG GCATCTGTGG TCTGTCCCTG 
ATGGGTGAAG CGGGTCTGCA GCCGGTGCAC GCGGCGCTCA ACATCAGCCT GCGGGCGTCC 
GACAGTCTGC GGCGGCTGCA CGAGTGCTGG AGGCGGAGCC GCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025426.1
CDS	41..1108
Translation	MADFESVDFLRERHVRFFQRCLYVLPERYAPYETSRLTIAFFSLSGLDVLGALDVVDRHSLIEWIYSLQILPTADQSNLQRCGFRGSSHIGVPYSSSKGPGAPHPYDSGHVTMTYTGLACLLILGDDLGRVDRAACVSGLRALQLEDGSFYAVPEGSENDMRFVYCAACICFMLDDWSGMDRQKTIDYIRRSTSFDFGIGQGAGLESHGGSTFCAVASLCMMGKLREVFSERELGRLRRWCVLRQQNGFQGRPNKPVDTCYSFWVGATLQLLDVFQYTNFEKNRNYILSTQDRLVGGFAKWPDSHPDPLHTYFGICGLSLMGEAGLQPVHAALNISLRASDSLRRLHECWRRSRT

Target ORF information:

RefSeq Version	NM_001030255.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein geranylgeranyltransferase type I, beta subunit (pggt1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030255.2

ORF Insert Sequence:

ATGGCGGATT TTGAGTCGGT GGATTTCCTC CGTGAGCGGC ACGTGCGCTT CTTCCAGCGC 
TGCCTGTACG TGTTACCGGA GAGATATGCG CCGTACGAGA CCAGCCGGCT GACGATAGCC 
TTCTTCTCTC TGTCGGGTCT GGATGTTCTG GGCGCTCTGG ATGTTGTGGA CAGACACAGT 
CTGATCGAGT GGATTTACTC TCTGCAGATC CTGCCCACCG CTGACCAGTC GAATCTCCAG 
CGCTGTGGCT TCAGAGGATC TTCCCACATT GGTGTTCCCT ACAGCTCGTC TAAGGGCCCC 
GGGGCTCCTC ACCCCTATGA CAGTGGGCAC GTGACCATGA CCTACACCGG GCTGGCGTGT 
CTGCTGATCT TGGGTGATGA TCTGGGCCGT GTGGATCGGG CCGCGTGTGT GTCGGGGCTG 
CGTGCGCTGC AGCTGGAGGA CGGCAGCTTC TACGCCGTGC CCGAGGGCAG CGAGAACGAC 
ATGCGCTTCG TCTACTGCGC CGCCTGCATC TGCTTCATGC TGGACGACTG GAGCGGGATG 
GACCGGCAGA AGACCATCGA CTACATCCGC AGGAGCACAT CGTTTGACTT CGGTATCGGG 
CAGGGCGCCG GCCTGGAGTC TCACGGTGGC TCCACCTTCT GTGCCGTGGC GTCGCTGTGC 
ATGATGGGAA AGCTGCGGGA GGTGTTCAGC GAGCGTGAGC TGGGTCGTCT GCGCCGCTGG 
TGTGTGCTCC GACAGCAGAA CGGCTTCCAG GGACGACCCA ACAAACCTGT GGACACCTGC 
TACTCCTTCT GGGTCGGGGC CACGCTGCAG CTGTTGGATG TGTTCCAGTA CACAAACTTT 
GAGAAGAACC GGAACTATAT TCTGTCCACT CAGGACCGGC TGGTGGGCGG CTTCGCTAAA 
TGGCCCGACA GTCACCCAGA CCCTCTCCAC ACCTACTTCG GCATCTGTGG TCTGTCCCTG 
ATGGGTGAAG CGGGTCTGCA GCCGGTGCAC GCGGCGCTCA ACATCAGCCT GCGGGCGTCC 
GACAGTCTGC GGCGGCTGCA CGAGTGCTGG AGGCGGAGCC GCACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Disruption of
Biology open6(3)348-357(2017 Mar)
El-Rass S,Eisa-Beygi S,Khong E,Brand-Arzamendi K,Mauro A,Zhang H,Clark KJ,Ekker SC,Wen XY

Evolutionary divergence of the vertebrate TNFAIP8 gene family: Applying the spotted gar orthology bridge to understand ohnolog loss in teleosts.
PloS one12(6)e0179517(2017)
Sullivan C,Lage CR,Yoder JA,Postlethwait JH,Kim CH

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

A zebrafish chemical suppressor screening identifies small molecule inhibitors of the Wnt/?-catenin pathway.
Chemistry & biology21(4)530-540(2014 Apr)
Nishiya N,Oku Y,Kumagai Y,Sato Y,Yamaguchi E,Sasaki A,Shoji M,Ohnishi Y,Okamoto H,Uehara Y

The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) pathway regulates developmental cerebral-vascular stability via prenylation-dependent signalling pathway.
Developmental biology373(2)258-66(2013 Jan)
Eisa-Beygi S,Hatch G,Noble S,Ekker M,Moon TW