Gna12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gna12
Entrez Gene ID	81663
Full Name	G protein subunit alpha 12
General protein information	Preferred Names G protein subunit alpha 12 Names guanine nucleotide-binding protein subunit alpha-12 g alpha-12 guanine nucleotide binding protein (G protein) alpha 12 guanine nucleotide binding protein, alpha 12
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q11
Summary	may play a role in D5 dopamine receptor signal transduction and renal sodium transport [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031034.2	NP_112296.1	guanine nucleotide-binding protein subunit alpha-12

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	gpa-12	NP_509557.1
Pan troglodytes (chimpanzee)	GNA12	XP_001137357.2
Bos taurus (cattle)	GNA12	NP_001179330.1
Homo sapiens (human)	GNA12	NP_031379.2
Canis lupus familiaris (dog)	GNA12	XP_005621208.1
Gallus gallus (chicken)	GNA12	XP_001233237.2
Danio rerio (zebrafish)	gna12a	NP_001013295.1
Mus musculus (house mouse)	Gna12	NP_034432.1
Rattus norvegicus (Norway rat)	Gna12	NP_112296.1

Gene Ontology
Bibliography

Related articles in PubMed

Stimulation of Dopamine D3 Receptor Attenuates Renal Ischemia-Reperfusion Injury via Increased Linkage With G?12.
Wang Z, Guan W, Han Y, Ren H, Tang X, Zhang H, Liu Y, Fu J, He D, Asico LD, Jose PA, Zhou L, Chen L, Zeng C
Transplantation99(11)2274-84(2015 Nov)

PRR5L degradation promotes mTORC2-mediated PKC-? phosphorylation and cell migration downstream of G?12.
Gan X, Wang J, Wang C, Sommer E, Kozasa T, Srinivasula S, Alessi D, Offermanns S, Simon MI, Wu D
Nature cell biology14(7)686-96(2012 May)

Role of G?(12)- and G?(13)-protein subunit linkage of D(3) dopamine receptors in the natriuretic effect of D(3) dopamine receptor in kidney.
Zhang Y, Fu C, Asico LD, Villar VA, Ren H, He D, Wang Z, Yang J, Jose PA, Zeng C
Hypertension research : official journal of the Japanese Society of Hypertension34(9)1011-6(2011 Sep)

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Kuo JC, Han X, Hsiao CT, Yates JR 3rd, Waterman CM
Nature cell biology13(4)383-93(2011 Apr)

Endothelial nitric oxide synthase inhibits G12/13 and rho-kinase activated by the angiotensin II type-1 receptor: implication in vascular migration.
Suzuki H, Kimura K, Shirai H, Eguchi K, Higuchi S, Hinoki A, Ishimaru K, Brailoiu E, Dhanasekaran DN, Stemmle LN, Fields TA, Frank GD, Autieri MV, Eguchi S
Arteriosclerosis, thrombosis, and vascular biology29(2)217-24(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Suggest that activation of D3R, by decreasing Galpha12-induced renal damage, may exert a protective effect from renal I/R injury.
Title: Stimulation of Dopamine D3 Receptor Attenuates Renal Ischemia-Reperfusion Injury via Increased Linkage With G?12.

It was concluded that a D3 receptor interaction with Galpha12/Galpha13 that increases sodium excretion may have a role in the regulation of blood pressure.
Title: Role of G?(12)- and G?(13)-protein subunit linkage of D(3) dopamine receptors in the natriuretic effect of D(3) dopamine receptor in kidney.

Endothelial nitric oxide synthase inhibits G12/13 and rho-kinase activated by the angiotensin II type-1 receptor: implication in vascular migration.
Title: Endothelial nitric oxide synthase inhibits G12/13 and rho-kinase activated by the angiotensin II type-1 receptor: implication in vascular migration.

thrombin and lysophosphatidic acid receptors couple to both gna12 and gna13 for growth cone collapse
Title: Lysophosphatidic acid and thrombin receptors require both G alpha12 and G alpha13 to regulate axonal morphology in hippocampal neurons.

Suggest that S1P(2) mediates inhibition of Rac and migration through the coordinated action of G(12/13) and G(q) for Rho activation in vascular smooth muscle.
Title: G12/13 and Gq mediate S1P2-induced inhibition of Rac and migration in vascular smooth muscle in a manner dependent on Rho but not Rho kinase.

The following Gna12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gna12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13298	NM_031034.2 Latest version!	Rattus norvegicus G protein subunit alpha 12 (Gna12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gna12 ( NM_031034.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112296.1 Rattus norvegicus G protein subunit alpha 12 (Gna12), mRNA.

Price & Availability↑

CloneID	ORa13298
Clone ID Related Accession (Same CDS sequence)	NM_031034.2
Accession Version	NM_031034.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein subunit alpha-12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07035471.1 and AC119536.6. On Sep 27, 2011 this sequence version replaced NM_031034.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D85760.1, DQ120479.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGGGG TGGTGCGGAC CCTCAGCCGC TGCTTGCTGC CGGCCGAGGC CGGAGCCCGC 
GAGCGCAGGG CGGGCGCGGC GCGCGACGCG GAACGCGAGG CCCGACGGCG CAGCCGCGAC 
ATCGACGCGC TGCTGGCCCG CGAGCGGCGC GCGGTGCGGC GGCTGGTCAA GATCTTGCTG 
CTGGGCGCCG GCGAGAGCGG CAAGTCCACC TTCCTCAAGC AGATGCGCAT CATCCACGGC 
CGGGAGTTCG ACCAGAAGGC GCTGCTGGAG TTCCGCGACA CCATCTTCGA CAACATCCTT 
AAGGGCTCGA GGGTGCTTGT AGACGCTCGG GACAAGCTTG GCATTCCCTG GCAGCACTCT 
GAGAACGAGA AGCACGGGAT GTTTCTGATG GCCTTCGAGA ACAAGGCAGG GCTGCCTGTG 
GAGCCTGCCA CCTTCCAGCT CTACGTTCCA GCCCTGAGCG CACTCTGGAG AGACTCCGGG 
ATCAGGGAAG CCTTCAGCCG CAGAAGCGAG TTCCAGCTGG GTGAGTCAGT GAAGTACTTC 
CTGGATAACT TAGACCGGAT TGGCCAGCTG AACTACTTCC CCAGTAAGCA AGACATCCTG 
TTGGCTAGAA AGGCCACCAA GGGAATCGTG GAGCATGACT TCGTGATAAA GAAAATCCCA 
TTTAAGATGG TGGATGTGGG CGGCCAGAGG TCGCAGCGCC AGAAGTGGTT CCAGTGCTTC 
GACGGGATCA CATCGATCCT GTTCATGGTG TCCTCAAGCG AGTATGACCA GGTCCTCATG 
GAGGATAGGC GCACCAACCG GCTGGTGGAG TCCATGAACA TCTTCGAGAC CATCGTCAAC 
AACAAGCTCT TCTTCAACGT CTCCATCATC CTCTTCCTCA ACAAGATGGA CCTGCTGGTG 
GAGAAGGTGA AGTCGGTGAG CATTAAGAAG CACTTCCCAG ACTTCAAGGG CGACCCGCAC 
CGGCTGGAGG ACGTCCAGCG CTACCTGGTC CAGTGCTTTG ACAGGAAGCG CAGGAACCGC 
GGCAAGCCTC TGTTCCACCA CTTCACCACT GCCATAGACA CCGAGAACAT CCGCTTCGTG 
TTTCATGCCG TGAAGGACAC CATCCTGCAG GAGAACCTGA AAGATATCAT GCTGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112296.1
CDS	34..1173
Translation	MSGVVRTLSRCLLPAEAGARERRAGAARDAEREARRRSRDIDALLARERRAVRRLVKILLLGAGESGKSTFLKQMRIIHGREFDQKALLEFRDTIFDNILKGSRVLVDARDKLGIPWQHSENEKHGMFLMAFENKAGLPVEPATFQLYVPALSALWRDSGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYFPSKQDILLARKATKGIVEHDFVIKKIPFKMVDVGGQRSQRQKWFQCFDGITSILFMVSSSEYDQVLMEDRRTNRLVESMNIFETIVNNKLFFNVSIILFLNKMDLLVEKVKSVSIKKHFPDFKGDPHRLEDVQRYLVQCFDRKRRNRGKPLFHHFTTAIDTENIRFVFHAVKDTILQENLKDIMLQ

Target ORF information:

RefSeq Version	NM_031034.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit alpha 12 (Gna12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031034.2

ORF Insert Sequence:

ATGTCCGGGG TGGTGCGGAC CCTCAGCCGC TGCTTGCTGC CGGCCGAGGC CGGAGCCCGC 
GAGCGCAGGG CGGGCGCGGC GCGCGACGCG GAACGCGAGG CCCGACGGCG CAGCCGCGAC 
ATCGACGCGC TGCTGGCCCG CGAGCGGCGC GCGGTGCGGC GGCTGGTCAA GATCTTGCTG 
CTGGGCGCCG GCGAGAGCGG CAAGTCCACC TTCCTCAAGC AGATGCGCAT CATCCACGGC 
CGGGAGTTCG ACCAGAAGGC GCTGCTGGAG TTCCGCGACA CCATCTTCGA CAACATCCTT 
AAGGGCTCGA GGGTGCTTGT AGACGCTCGG GACAAGCTTG GCATTCCCTG GCAGCACTCT 
GAGAACGAGA AGCACGGGAT GTTTCTGATG GCCTTCGAGA ACAAGGCAGG GCTGCCTGTG 
GAGCCTGCCA CCTTCCAGCT CTACGTTCCA GCCCTGAGCG CACTCTGGAG AGACTCCGGG 
ATCAGGGAAG CCTTCAGCCG CAGAAGCGAG TTCCAGCTGG GTGAGTCAGT GAAGTACTTC 
CTGGATAACT TAGACCGGAT TGGCCAGCTG AACTACTTCC CCAGTAAGCA AGACATCCTG 
TTGGCTAGAA AGGCCACCAA GGGAATCGTG GAGCATGACT TCGTGATAAA GAAAATCCCA 
TTTAAGATGG TGGATGTGGG CGGCCAGAGG TCGCAGCGCC AGAAGTGGTT CCAGTGCTTC 
GACGGGATCA CATCGATCCT GTTCATGGTG TCCTCAAGCG AGTATGACCA GGTCCTCATG 
GAGGATAGGC GCACCAACCG GCTGGTGGAG TCCATGAACA TCTTCGAGAC CATCGTCAAC 
AACAAGCTCT TCTTCAACGT CTCCATCATC CTCTTCCTCA ACAAGATGGA CCTGCTGGTG 
GAGAAGGTGA AGTCGGTGAG CATTAAGAAG CACTTCCCAG ACTTCAAGGG CGACCCGCAC 
CGGCTGGAGG ACGTCCAGCG CTACCTGGTC CAGTGCTTTG ACAGGAAGCG CAGGAACCGC 
GGCAAGCCTC TGTTCCACCA CTTCACCACT GCCATAGACA CCGAGAACAT CCGCTTCGTG 
TTTCATGCCG TGAAGGACAC CATCCTGCAG GAGAACCTGA AAGATATCAT GCTGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Stimulation of Dopamine D3 Receptor Attenuates Renal Ischemia-Reperfusion Injury via Increased Linkage With G?12.
Transplantation99(11)2274-84(2015 Nov)
Wang Z,Guan W,Han Y,Ren H,Tang X,Zhang H,Liu Y,Fu J,He D,Asico LD,Jose PA,Zhou L,Chen L,Zeng C

PRR5L degradation promotes mTORC2-mediated PKC-? phosphorylation and cell migration downstream of G?12.
Nature cell biology14(7)686-96(2012 May)
Gan X,Wang J,Wang C,Sommer E,Kozasa T,Srinivasula S,Alessi D,Offermanns S,Simon MI,Wu D

Role of G?(12)- and G?(13)-protein subunit linkage of D(3) dopamine receptors in the natriuretic effect of D(3) dopamine receptor in kidney.
Hypertension research : official journal of the Japanese Society of Hypertension34(9)1011-6(2011 Sep)
Zhang Y,Fu C,Asico LD,Villar VA,Ren H,He D,Wang Z,Yang J,Jose PA,Zeng C

Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for ?-Pix in negative regulation of focal adhesion maturation.
Nature cell biology13(4)383-93(2011 Apr)
Kuo JC,Han X,Hsiao CT,Yates JR 3rd,Waterman CM

Endothelial nitric oxide synthase inhibits G12/13 and rho-kinase activated by the angiotensin II type-1 receptor: implication in vascular migration.
Arteriosclerosis, thrombosis, and vascular biology29(2)217-24(2009 Feb)
Suzuki H,Kimura K,Shirai H,Eguchi K,Higuchi S,Hinoki A,Ishimaru K,Brailoiu E,Dhanasekaran DN,Stemmle LN,Fields TA,Frank GD,Autieri MV,Eguchi S

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Gna12 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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