Fstl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fstl1
Entrez Gene ID	79210
Full Name	follistatin-like 1
Synonyms	Frp,Fstl
General protein information	Preferred Names follistatin-like 1 Names follistatin-related protein 1 follistatin-like protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q21
Summary	interacts with transforming growth factor (TGF) superfamily proteins; may play a role in reproduction and in regulation of activin A in brain wound healing [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_024369.2	NP_077345.1	follistatin-related protein 1 precursor
XM_017598075.1	XP_017453564.1	follistatin-related protein 1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Fstl1	NP_032073.2
Danio rerio (zebrafish)	fstl1a	NP_001017860.1
Bos taurus (cattle)	FSTL1	NP_001017950.1
Rattus norvegicus (Norway rat)	Fstl1	NP_077345.1
Danio rerio (zebrafish)	fstl1b	NP_001034710.1
Xenopus tropicalis (tropical clawed frog)	fstl1	NP_001039221.1
Pan troglodytes (chimpanzee)	FSTL1	NP_001231517.1
Canis lupus familiaris (dog)	FSTL1	XP_005639705.1
Homo sapiens (human)	FSTL1	NP_009016.1
Gallus gallus (chicken)	FSTL1	XP_004938226.1

Gene Ontology
Bibliography

Related articles in PubMed

Follistatin-like 1 expression is decreased in the alveolar epithelium of hypoplastic rat lungs with nitrofen-induced congenital diaphragmatic hernia.
Takahashi T, Zimmer J, Friedmacher F, Puri P
Journal of pediatric surgery52(5)706-709(2017 May)

FSTL1 as a Potential Mediator of Exercise-Induced Cardioprotection in Post-Myocardial Infarction Rats.
Xi Y, Gong DW, Tian Z
Scientific reports632424(2016 Aug)

Follistatin-like 1 protects cardiomyoblasts from injury induced by sodium nitroprusside through modulating Akt and Smad1/5/9 signaling.
Chen W, Xia J, Hu P, Zhou F, Chen Y, Wu J, Lei W, Shen Z
Biochemical and biophysical research communications469(3)418-23(2016 Jan)

Follistatin-like 1 attenuates apoptosis via disco-interacting protein 2 homolog A/Akt pathway after middle cerebral artery occlusion in rats.
Liang X, Hu Q, Li B, McBride D, Bian H, Spagnoli P, Chen D, Tang J, Zhang JH
Stroke45(10)3048-3054(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Decreased expression of Fstl1 in alveolar epithelium may disrupt alveolarization and pulmonary surfactant production, thus contributing to the development of pulmonary hypertension in the nitrofen-induced rat congenital diaphragmatic hernia model.
Title: Follistatin-like 1 expression is decreased in the alveolar epithelium of hypoplastic rat lungs with nitrofen-induced congenital diaphragmatic hernia.

Taken together, these results indicated that FSTL1 protects the SNP-induced injury in cardiac H9c2 cells through, at least in part, the activation of Akt and inhibition of Smad1/5/9 signaling.
Title: Follistatin-like 1 protects cardiomyoblasts from injury induced by sodium nitroprusside through modulating Akt and Smad1/5/9 signaling.

our findings indicate that FSTL1 may contribute to neuronal survival after brain ischemia
Title: Follistatin-like 1 attenuates apoptosis via disco-interacting protein 2 homolog A/Akt pathway after middle cerebral artery occlusion in rats.

Follistatin-like 1 (FSTL1) transcript is overexpressed in tolerated allografts compared to rejected allografts or syngeneic grafts
Title: An immunomodulatory role for follistatin-like 1 in heart allograft transplantation.

Expression levels of FLRG protein are significantly increased in brain damaged regions after penetrating injury; specifically, FLRG protein is colocalized in glial fibrillary acidic protein-positive reactive astroglial cells.
Title: Regulation of FLRG expression in rat primary astroglial cells and injured brain tissue by transforming growth factor-beta 1 (TGF-beta 1).

The following Fstl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fstl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06956	NM_024369.2 Latest version!	Rattus norvegicus follistatin-like 1 (Fstl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa57723	XM_017598075.1 Latest version!	Rattus norvegicus follistatin-like 1 (Fstl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fstl1 ( NM_024369.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077345.1 Rattus norvegicus follistatin-like 1 (Fstl1), mRNA.

Price & Availability↑

CloneID	ORa06956
Clone ID Related Accession (Same CDS sequence)	NM_024369.2
Accession Version	NM_024369.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 921bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Rattus norvegicus(Norway rat)
Product	follistatin-related protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC087014.1. On Dec 26, 2004 this sequence version replaced NM_024369.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC087014.1, U06864.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTGGAAAC GCTGGCTGGC GCTCGCGCTG GTGACCATCG CCCTGGTCCA CGGCGAGGAG 
GAACAAAGAA GCAAATCCAA GATCTGCGCC AATGTGTTTT GTGGAGCTGG CCGGGAATGC 
GCCGTCACGG AGAAGGGGGA GCCAACGTGC CTCTGCATTG AGCAATGCAA ACCTCACAAG 
AGGCCTGTGT GTGGCAGTAA TGGCAAGACC TACCTCAACC ATTGTGAACT TCACAGAGAC 
GCCTGCCTCA CTGGATCCAA GATCCAGGTT GATTATGATG GGCACTGCAA AGAAAAGAAG 
TCTGTGAGTC CATCCGCCAG CCCCGTTGTC TGCTATCAGG CTAACCGTGA TGAGCTGCGG 
CGCCGGATCA TCCAGTGGCT GGAAGCCGAG ATCATTCCAG ATGGCTGGTT CTCTAAAGGC 
AGTAACTACA GTGAGATCCT AGACAAGTAC TTTAAGAGCT TTGATAATGG TGACTCTCAC 
CTGGACTCCA GCGAATTCCT GAAATTCGTG GAGCAGAATG AAACAGCCGT CAACATCACC 
GCTTACCCCA ATCAGGAGAA CAACAAACTG CTCAGAGGCC TCTGTGTTGA TGCCCTCATT 
GAACTGTCCG ATGAGAACGC TGACTGGAAA CTCAGCTTCC AAGAGTTCCT CAAGTGCCTC 
AACCCATCCT TCAACCCTCC TGAGAAGAAG TGCGCCCTGG AGGACGAAAC CTATGCAGAT 
GGAGCTGAGA CCGAGGTGGA CTGCAATCGC TGTGTCTGTT CCTGTGGACA CTGGGTCTGC 
ACAGCGATGA CCTGTGATGG AAAGAATCAG AAGGGGGTCC AGACCCACAC AGAGGAGGAG 
ATGACGAGAT ATGCCCAGGA ACTCCAGAAG CACCAGGGAA CAGCAGAAAA GACCAAGAAG 
GTGAACACCA AAGAGATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077345.1
CDS	89..1009
Translation	MWKRWLALALVTIALVHGEEEQRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSVSPSASPVVCYQANRDELRRRIIQWLEAEIIPDGWFSKGSNYSEILDKYFKSFDNGDSHLDSSEFLKFVEQNETAVNITAYPNQENNKLLRGLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCSCGHWVCTAMTCDGKNQKGVQTHTEEEMTRYAQELQKHQGTAEKTKKVNTKEI

Target ORF information:

RefSeq Version	NM_024369.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus follistatin-like 1 (Fstl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024369.2

ORF Insert Sequence:

ATGTGGAAAC GCTGGCTGGC GCTCGCGCTG GTGACCATCG CCCTGGTCCA CGGCGAGGAG 
GAACAAAGAA GCAAATCCAA GATCTGCGCC AATGTGTTTT GTGGAGCTGG CCGGGAATGC 
GCCGTCACGG AGAAGGGGGA GCCAACGTGC CTCTGCATTG AGCAATGCAA ACCTCACAAG 
AGGCCTGTGT GTGGCAGTAA TGGCAAGACC TACCTCAACC ATTGTGAACT TCACAGAGAC 
GCCTGCCTCA CTGGATCCAA GATCCAGGTT GATTATGATG GGCACTGCAA AGAAAAGAAG 
TCTGTGAGTC CATCCGCCAG CCCCGTTGTC TGCTATCAGG CTAACCGTGA TGAGCTGCGG 
CGCCGGATCA TCCAGTGGCT GGAAGCCGAG ATCATTCCAG ATGGCTGGTT CTCTAAAGGC 
AGTAACTACA GTGAGATCCT AGACAAGTAC TTTAAGAGCT TTGATAATGG TGACTCTCAC 
CTGGACTCCA GCGAATTCCT GAAATTCGTG GAGCAGAATG AAACAGCCGT CAACATCACC 
GCTTACCCCA ATCAGGAGAA CAACAAACTG CTCAGAGGCC TCTGTGTTGA TGCCCTCATT 
GAACTGTCCG ATGAGAACGC TGACTGGAAA CTCAGCTTCC AAGAGTTCCT CAAGTGCCTC 
AACCCATCCT TCAACCCTCC TGAGAAGAAG TGCGCCCTGG AGGACGAAAC CTATGCAGAT 
GGAGCTGAGA CCGAGGTGGA CTGCAATCGC TGTGTCTGTT CCTGTGGACA CTGGGTCTGC 
ACAGCGATGA CCTGTGATGG AAAGAATCAG AAGGGGGTCC AGACCCACAC AGAGGAGGAG 
ATGACGAGAT ATGCCCAGGA ACTCCAGAAG CACCAGGGAA CAGCAGAAAA GACCAAGAAG 
GTGAACACCA AAGAGATCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fstl1 ( XM_017598075.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453564.1 Rattus norvegicus follistatin-like 1 (Fstl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa57723
Clone ID Related Accession (Same CDS sequence)	XM_017598075.1
Accession Version	XM_017598075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	follistatin-related protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACACCC CGTGCCTTCT CTTGAGATAT CTGAGGATTC TTGGTGGGGG TCTAGGAGAG 
GACATGGATG CCTGCGGTGC ACGGAGGCTC CTGGGCTCCG GGCGGGGGCC AGGAGGAGGG 
AGCGCAGCGG GAGCCGCGGG GGCCGCGCTG CAGAAGGGGA GGAGAATGGG GAGGAGCTGG 
GGGGCCGGCG GGCGGGGAAG GAGGGGTCTT AAGGGGCGGC GAGCCCAGGT CGGACTTCCT 
CGGCGCCTGG TGATCGGCGA CGCTCCCACC TTCGCCTCCA ACTCACTGCT TCCATCCTGC 
CCAGTGTCCT CTCGAGTCCC GGACCCGAGC ACGATGTGGA AACGCTGGCT GGCGCTCGCG 
CTGGTGACCA TCGCCCTGGT CCACGGCGAG GAGGAACAAA GAAGCAAATC CAAGATCTGC 
GCCAATGTGT TTTGTGGAGC TGGCCGGGAA TGCGCCGTCA CGGAGAAGGG GGAGCCAACG 
TGCCTCTGCA TTGAGCAATG CAAACCTCAC AAGAGGCCTG TGTGTGGCAG TAATGGCAAG 
ACCTACCTCA ACCATTGTGA ACTTCACAGA GACGCCTGCC TCACTGGATC CAAGATCCAG 
GTTGATTATG ATGGGCACTG CAAAGAAAAG AAGTCTGTGA GTCCATCCGC CAGCCCCGTT 
GTCTGCTATC AGGCTAACCG TGATGAGCTG CGGCGCCGGA TCATCCAGTG GCTGGAAGCC 
GAGATCATTC CAGATGGCTG GTTCTCTAAA GGCAGTAACT ACAGTGAGAT CCTAGACAAG 
TACTTTAAGA GCTTTGATAA TGGTGACTCT CACCTGGACT CCAGCGAATT CCTGAAATTC 
GTGGAGCAGA ATGAAACAGC CGTCAACATC ACCGCTTACC CCAATCAGGA GAACAACAAA 
CTGCTCAGAG GCCTCTGTGT TGATGCCCTC ATTGAACTGT CCGATGAGAA CGCTGACTGG 
AAACTCAGCT TCCAAGAGTT CCTCAAGTGC CTCAACCCAT CCTTCAACCC TCCTGAGAAG 
AAGTGCGCCC TGGAGGACGA AACCTATGCA GATGGAGCTG AGACCGAGGT GGACTGCAAT 
CGCTGTGTCT GTTCCTGTGG ACACTGGGTC TGCACAGCGA TGACCTGTGA TGGAAAGAAT 
CAGAAGGGGG TCCAGACCCA CACAGAGGAG GAGATGACGA GATATGCCCA GGAACTCCAG 
AAGCACCAGG GAACAGCAGA AAAGACCAAG AAGGTGAACA CCAAAGAGAT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453564.1
CDS	102..1355
Translation	MDTPCLLLRYLRILGGGLGEDMDACGARRLLGSGRGPGGGSAAGAAGAALQKGRRMGRSWGAGGRGRRGLKGRRAQVGLPRRLVIGDAPTFASNSLLPSCPVSSRVPDPSTMWKRWLALALVTIALVHGEEEQRSKSKICANVFCGAGRECAVTEKGEPTCLCIEQCKPHKRPVCGSNGKTYLNHCELHRDACLTGSKIQVDYDGHCKEKKSVSPSASPVVCYQANRDELRRRIIQWLEAEIIPDGWFSKGSNYSEILDKYFKSFDNGDSHLDSSEFLKFVEQNETAVNITAYPNQENNKLLRGLCVDALIELSDENADWKLSFQEFLKCLNPSFNPPEKKCALEDETYADGAETEVDCNRCVCSCGHWVCTAMTCDGKNQKGVQTHTEEEMTRYAQELQKHQGTAEKTKKVNTKEI

Target ORF information:

RefSeq Version	XM_017598075.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus follistatin-like 1 (Fstl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598075.1

ORF Insert Sequence:

ATGGACACCC CGTGCCTTCT CTTGAGATAT CTGAGGATTC TTGGTGGGGG TCTAGGAGAG 
GACATGGATG CCTGCGGTGC ACGGAGGCTC CTGGGCTCCG GGCGGGGGCC AGGAGGAGGG 
AGCGCAGCGG GAGCCGCGGG GGCCGCGCTG CAGAAGGGGA GGAGAATGGG GAGGAGCTGG 
GGGGCCGGCG GGCGGGGAAG GAGGGGTCTT AAGGGGCGGC GAGCCCAGGT CGGACTTCCT 
CGGCGCCTGG TGATCGGCGA CGCTCCCACC TTCGCCTCCA ACTCACTGCT TCCATCCTGC 
CCAGTGTCCT CTCGAGTCCC GGACCCGAGC ACGATGTGGA AACGCTGGCT GGCGCTCGCG 
CTGGTGACCA TCGCCCTGGT CCACGGCGAG GAGGAACAAA GAAGCAAATC CAAGATCTGC 
GCCAATGTGT TTTGTGGAGC TGGCCGGGAA TGCGCCGTCA CGGAGAAGGG GGAGCCAACG 
TGCCTCTGCA TTGAGCAATG CAAACCTCAC AAGAGGCCTG TGTGTGGCAG TAATGGCAAG 
ACCTACCTCA ACCATTGTGA ACTTCACAGA GACGCCTGCC TCACTGGATC CAAGATCCAG 
GTTGATTATG ATGGGCACTG CAAAGAAAAG AAGTCTGTGA GTCCATCCGC CAGCCCCGTT 
GTCTGCTATC AGGCTAACCG TGATGAGCTG CGGCGCCGGA TCATCCAGTG GCTGGAAGCC 
GAGATCATTC CAGATGGCTG GTTCTCTAAA GGCAGTAACT ACAGTGAGAT CCTAGACAAG 
TACTTTAAGA GCTTTGATAA TGGTGACTCT CACCTGGACT CCAGCGAATT CCTGAAATTC 
GTGGAGCAGA ATGAAACAGC CGTCAACATC ACCGCTTACC CCAATCAGGA GAACAACAAA 
CTGCTCAGAG GCCTCTGTGT TGATGCCCTC ATTGAACTGT CCGATGAGAA CGCTGACTGG 
AAACTCAGCT TCCAAGAGTT CCTCAAGTGC CTCAACCCAT CCTTCAACCC TCCTGAGAAG 
AAGTGCGCCC TGGAGGACGA AACCTATGCA GATGGAGCTG AGACCGAGGT GGACTGCAAT 
CGCTGTGTCT GTTCCTGTGG ACACTGGGTC TGCACAGCGA TGACCTGTGA TGGAAAGAAT 
CAGAAGGGGG TCCAGACCCA CACAGAGGAG GAGATGACGA GATATGCCCA GGAACTCCAG 
AAGCACCAGG GAACAGCAGA AAAGACCAAG AAGGTGAACA CCAAAGAGAT CTAA