Mvp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mvp
Entrez Gene ID	64681
Full Name	major vault protein
General protein information	Preferred Names major vault protein Names major vault protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q37
Summary	member of a large cytosolic ribonucleoprotein particle; may play a role in vault function [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022715.2	NP_073206.2	major vault protein
XM_017589686.1	XP_017445175.1	major vault protein isoform X1

Pathways from BioSystems

Homologies

	LOC706736	XP_002808339.1
Rattus norvegicus (Norway rat)	Mvp	NP_073206.2
Danio rerio (zebrafish)	mvp	NP_958482.1
Canis lupus familiaris (dog)	MVP	XP_536910.3
Bos taurus (cattle)	MVP	NP_001030394.1
Gallus gallus (chicken)	MVP	NP_001006336.1
Xenopus tropicalis (tropical clawed frog)	mvp	NP_001072293.1
Homo sapiens (human)	MVP	NP_005106.2
Mus musculus (house mouse)	Mvp	NP_542369.2

Gene Ontology
Bibliography

Related articles in PubMed

Modulation of the Vault Protein-Protein Interaction for Tuning of Molecular Release.
Yu K, Yau YH, Sinha A, Tan T, Kickhoefer VA, Rome LH, Lee H, Shochat SG, Lim S
Scientific reports7(1)14816(2017 Nov)

Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
Corominas R, Yang X, Lin GN, Kang S, Shen Y, Ghamsari L, Broly M, Rodriguez M, Tam S, Trigg SA, Fan C, Yi S, Tasan M, Lemmens I, Kuang X, Zhao N, Malhotra D, Michaelson JJ, Vacic V, Calderwood MA, Roth FP, Tavernier J, Horvath S, Salehi-Ashtiani K, Korkin D, Sebat J, Hill DE, Hao T, Vidal M, Iakoucheva LM
Nature communications53650(2014 Apr)

Toward an understanding of the protein interaction network of the human liver.
Wang J, Huo K, Ma L, Tang L, Li D, Huang X, Yuan Y, Li C, Wang W, Guan W, Chen H, Jin C, Wei J, Zhang W, Yang Y, Liu Q, Zhou Y, Zhang C, Wu Z, Xu W, Zhang Y, Liu T, Yu D, Zhang Y, Chen L, Zhu D, Zhong X, Kang L, Gan X, Yu X, Ma Q, Yan J, Zhou L, Liu Z, Zhu Y, Zhou T, He F, Yang X
Molecular systems biology7536(2011 Oct)

Protein profile of exosomes from trabecular meshwork cells.
Stamer WD, Hoffman EA, Luther JM, Hachey DL, Schey KL
Journal of proteomics74(6)796-804(2011 May)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

x-ray structure of liver vault at 3.5 angstrom resolution; study shows the cage structure consists of a dimer of half-vaults, with each half-vault comprising 39 identical major vault protein (MVP) chains; each MVP monomer folds into 12 domains
Title: The structure of rat liver vault at 3.5 angstrom resolution.

Data show that the interaction between Src and major vault protein (MVP) is critically dependent on Src activity and protein (MVP) tyrosyl phosphorylation, which are induced by epidermal growth factor stimulation.
Title: Crosstalk between Src and major vault protein in epidermal growth factor-dependent cell signalling.

casein kinase II enhances the phosphorylation of MVP in PC12 cells.Our results suggest that cell-specific phosphorylation of MVP may play a critical role in vault function.
Title: Major vault protein is a substrate of endogenous protein kinases in CHO and PC12 cells.

The following Mvp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mvp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa56829	XM_017589686.1 Latest version!	Rattus norvegicus major vault protein (Mvp), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$475.30 $679.00
ORa12977	NM_022715.2 Latest version!	Rattus norvegicus major vault protein (Mvp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$503.30 $719.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mvp ( XM_017589686.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445175.1 Rattus norvegicus major vault protein (Mvp), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56829
Clone ID Related Accession (Same CDS sequence)	XM_017589686.1
Accession Version	XM_017589686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	major vault protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGTG GGAAAAGCTT GGGATTCACC ATGGCAACTG AAGAGGCCAT CATCCGCATC 
CCCCCATACC ACTACATCCA TGTGCTGGAC CAGAACAGTA ATGTGTCCCG TGTGGAGGTT 
GGACCAAAGA CCTACATCCG GCAGGACAAT GAGAGGGTAC TGTTTGCCCC AGTTCGCATG 
GTGACCGTCC CCCCACGCCA CTACTGCATA GTGGCCAACC CTGTGTCCCG GGACACCCAG 
AGTTCTGTGT TATTTGACAT CACAGGACAA GTCCGACTCC GGCACGCTGA CCAGGAGATC 
CGACTAGCCC AGGACCCCTT CCCCCTGTAT CCAGGGGAGG TGCTGGAAAA GGACATCACC 
CCACTGCAGG TGGTTCTGCC CAACACAGCA CTGCATCTTA AGGCGTTGCT GGACTTTGAG 
GATAAGAATG GAGACAAGGT CATGGCAGGA GACGAGTGGC TATTTGAGGG ACCTGGCACC 
TACATCCCAC AGAAGGAAGT GGAAGTCGTG GAGATCATTC AGGCCACAGT CATCAAACAG 
AACCAAGCAC TGCGGCTAAG GGCCCGAAAG GAGTGCTTTG ACCGGGAGGG CAAGGGGCGC 
GTGACAGGTG AGGAGTGGCT GGTCCGATCC GTGGGGGCTT ACCTCCCAGC TGTCTTTGAA 
GAGGTGCTGG ATCTGGTGGA TGCTGTGATC CTTACAGAAA AGACTGCCCT GCACCTCCGG 
GCTCTGCAGA ACTTCAGGGA CCTTCGGGGA GTGCTCCACC GCACCGGGGA GGAATGGTTA 
GTGACGGTGC AGGACACAGA AGCCCATGTT CCAGATGTCT ATGAGGAGGT GCTTGGGGTA 
GTACCCATCA CCACCCTGGG ACCTCGACAC TACTGTGTCA TTCTTGACCC AATGGGACCA 
GACGGCAAGA ACCAGCTGGG ACAAAAGCGT GTTGTCAAGG GAGAGAAGTC CTTTTTCCTC 
CAGCCAGGAG AGAGGCTGGA GCGAGGCATC CAGGATGTGT ATGTGCTGTC AGAGCAGCAG 
GGGCTGCTAC TGAAGGCACT GCAGCCCCTG GAGGAGGGAG AGAGCGAGGA GAAGGTCTCC 
CATCAGGCCG GAGACTGCTG GCTCATCCGT GGGCCCCTGG AGTATGTGCC ATCTGCAAAA 
GTGGAGGTGG TGGAGGAGCG TCAGGCTATC CCTCTGGACC AAAATGAGGG CATCTATGTG 
CAGGATGTCA AGACGGGGAA GGTGCGGGCT GTGATTGGAA GCACCTACAT GCTGACTCAG 
GATGAAGTCC TGTGGGAAAA GGAGCTGCCT TCTGGGGTGG AGGAGCTGCT GAACTTGGGG 
CATGACCCTC TGGCAGACAG GGGTCAGAAG GGCACAGCCA AGCCCCTTCA GCCCTCAGCT 
CCAAGGAACA AGACCCGAGT GGTCAGCTAC CGTGTCCCGC ACAATGCAGC GGTGCAGGTC 
TATGACTACA GAGCCAAGAG AGCCCGTGTG GTCTTTGGGC CCGAGCTAGT GACACTGGAT 
CCTGAGGAGC AGTTCACAGT ATTGTCCCTT TCTGCCGGGC GACCCAAGCG TCCTCATGCC 
CGCCGTGCAC TCTGCCTACT GCTGGGACCT GATTTCTTTA CTGATGTCAT CACCATCGAA 
ACTGCAGATC ATGCCAGGTT GCAGCTGCAG CTTGCCTACA ACTGGCACTT TGAACTGAAG 
AACCGGAATG ACCCTGCAGA GGCAGCCAAG CTTTTCTCCG TGCCTGACTT CGTGGGTGAC 
GCCTGCAAGG CCATTGCATC CCGAGTCCGG GGGGCTGTAG CCTCTGTCAC CTTTGATGAC 
TTCCATAAAA ACTCAGCCCG GATCATTCGA ATGGCTGTTT TTGGCTTTGA GATGTCTGAA 
GACACAGGTC CTGATGGCAC ACTCCTGCCC AAGGCTCGAG ACCAGGCAGT CTTTCCCCAA 
AACGGGCTGG TAGTCAGCAG TGTGGATGTG CAGTCAGTGG AGCCCGTGGA CCAGAGGACC 
CGGGATGCCC TTCAGCGCAG CGTTCAGCTG GCCATCGAAA TTACCACCAA CTCCCAGGAG 
GCAGCAGCCA AGCACGAGGC TCAGAGACTG GAACAGGAAG CCCGTGGTCG GCTTGAGAGG 
CAGAAGATCT TGGACCAGTC AGAAGCTGAA AAAGCCCGCA AGGAACTCTT GGAGCTTGAG 
GCTATGAGCA TGGCTGTGGA GAGCACGGGT AATGCCAAAG CAGAGGCTGA GTCCCGTGCA 
GAGGCAGCGA GGATCGAAGG AGAAGGCTCT GTGCTGCAGG CCAAGCTCAA GGCACAGGCG 
CTAGCCATTG AGACGGAGGC TGAGTTGGAG CGAGTAAAGA AAGTACGAGA GATGGAACTG 
ATCTATGCCC GGGCCCAGTT GGAGCTGGAG GTGAGCAAGG CGCAGCAGCT TGCCAATGTG 
GAGGCAAAGA AGTTCAAGGA GATGACAGAG GCACTGGGCC CCGGCACCAT CAGGGACCTG 
GCTGTGGCCG GGCCAGAGAT GCAGGTGAAA CTTCTCCAGT CCCTGGGCCT GAAATCCACT 
CTCATCACCG ATGGCTCGTC TCCCATCAAC CTCTTCAGCA CAGCCTTCGG GTTGCTGGGG 
CTGGGGTCTG ATGGTCAGCC GCCAGCACAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445175.1
CDS	226..2841
Translation	MFGGKSLGFTMATEEAIIRIPPYHYIHVLDQNSNVSRVEVGPKTYIRQDNERVLFAPVRMVTVPPRHYCIVANPVSRDTQSSVLFDITGQVRLRHADQEIRLAQDPFPLYPGEVLEKDITPLQVVLPNTALHLKALLDFEDKNGDKVMAGDEWLFEGPGTYIPQKEVEVVEIIQATVIKQNQALRLRARKECFDREGKGRVTGEEWLVRSVGAYLPAVFEEVLDLVDAVILTEKTALHLRALQNFRDLRGVLHRTGEEWLVTVQDTEAHVPDVYEEVLGVVPITTLGPRHYCVILDPMGPDGKNQLGQKRVVKGEKSFFLQPGERLERGIQDVYVLSEQQGLLLKALQPLEEGESEEKVSHQAGDCWLIRGPLEYVPSAKVEVVEERQAIPLDQNEGIYVQDVKTGKVRAVIGSTYMLTQDEVLWEKELPSGVEELLNLGHDPLADRGQKGTAKPLQPSAPRNKTRVVSYRVPHNAAVQVYDYRAKRARVVFGPELVTLDPEEQFTVLSLSAGRPKRPHARRALCLLLGPDFFTDVITIETADHARLQLQLAYNWHFELKNRNDPAEAAKLFSVPDFVGDACKAIASRVRGAVASVTFDDFHKNSARIIRMAVFGFEMSEDTGPDGTLLPKARDQAVFPQNGLVVSSVDVQSVEPVDQRTRDALQRSVQLAIEITTNSQEAAAKHEAQRLEQEARGRLERQKILDQSEAEKARKELLELEAMSMAVESTGNAKAEAESRAEAARIEGEGSVLQAKLKAQALAIETEAELERVKKVREMELIYARAQLELEVSKAQQLANVEAKKFKEMTEALGPGTIRDLAVAGPEMQVKLLQSLGLKSTLITDGSSPINLFSTAFGLLGLGSDGQPPAQK

Target ORF information:

RefSeq Version	XM_017589686.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus major vault protein (Mvp), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589686.1

ORF Insert Sequence:

ATGTTTGGTG GGAAAAGCTT GGGATTCACC ATGGCAACTG AAGAGGCCAT CATCCGCATC 
CCCCCATACC ACTACATCCA TGTGCTGGAC CAGAACAGTA ATGTGTCCCG TGTGGAGGTT 
GGACCAAAGA CCTACATCCG GCAGGACAAT GAGAGGGTAC TGTTTGCCCC AGTTCGCATG 
GTGACCGTCC CCCCACGCCA CTACTGCATA GTGGCCAACC CTGTGTCCCG GGACACCCAG 
AGTTCTGTGT TATTTGACAT CACAGGACAA GTCCGACTCC GGCACGCTGA CCAGGAGATC 
CGACTAGCCC AGGACCCCTT CCCCCTGTAT CCAGGGGAGG TGCTGGAAAA GGACATCACC 
CCACTGCAGG TGGTTCTGCC CAACACAGCA CTGCATCTTA AGGCGTTGCT GGACTTTGAG 
GATAAGAATG GAGACAAGGT CATGGCAGGA GACGAGTGGC TATTTGAGGG ACCTGGCACC 
TACATCCCAC AGAAGGAAGT GGAAGTCGTG GAGATCATTC AGGCCACAGT CATCAAACAG 
AACCAAGCAC TGCGGCTAAG GGCCCGAAAG GAGTGCTTTG ACCGGGAGGG CAAGGGGCGC 
GTGACAGGTG AGGAGTGGCT GGTCCGATCC GTGGGGGCTT ACCTCCCAGC TGTCTTTGAA 
GAGGTGCTGG ATCTGGTGGA TGCTGTGATC CTTACAGAAA AGACTGCCCT GCACCTCCGG 
GCTCTGCAGA ACTTCAGGGA CCTTCGGGGA GTGCTCCACC GCACCGGGGA GGAATGGTTA 
GTGACGGTGC AGGACACAGA AGCCCATGTT CCAGATGTCT ATGAGGAGGT GCTTGGGGTA 
GTACCCATCA CCACCCTGGG ACCTCGACAC TACTGTGTCA TTCTTGACCC AATGGGACCA 
GACGGCAAGA ACCAGCTGGG ACAAAAGCGT GTTGTCAAGG GAGAGAAGTC CTTTTTCCTC 
CAGCCAGGAG AGAGGCTGGA GCGAGGCATC CAGGATGTGT ATGTGCTGTC AGAGCAGCAG 
GGGCTGCTAC TGAAGGCACT GCAGCCCCTG GAGGAGGGAG AGAGCGAGGA GAAGGTCTCC 
CATCAGGCCG GAGACTGCTG GCTCATCCGT GGGCCCCTGG AGTATGTGCC ATCTGCAAAA 
GTGGAGGTGG TGGAGGAGCG TCAGGCTATC CCTCTGGACC AAAATGAGGG CATCTATGTG 
CAGGATGTCA AGACGGGGAA GGTGCGGGCT GTGATTGGAA GCACCTACAT GCTGACTCAG 
GATGAAGTCC TGTGGGAAAA GGAGCTGCCT TCTGGGGTGG AGGAGCTGCT GAACTTGGGG 
CATGACCCTC TGGCAGACAG GGGTCAGAAG GGCACAGCCA AGCCCCTTCA GCCCTCAGCT 
CCAAGGAACA AGACCCGAGT GGTCAGCTAC CGTGTCCCGC ACAATGCAGC GGTGCAGGTC 
TATGACTACA GAGCCAAGAG AGCCCGTGTG GTCTTTGGGC CCGAGCTAGT GACACTGGAT 
CCTGAGGAGC AGTTCACAGT ATTGTCCCTT TCTGCCGGGC GACCCAAGCG TCCTCATGCC 
CGCCGTGCAC TCTGCCTACT GCTGGGACCT GATTTCTTTA CTGATGTCAT CACCATCGAA 
ACTGCAGATC ATGCCAGGTT GCAGCTGCAG CTTGCCTACA ACTGGCACTT TGAACTGAAG 
AACCGGAATG ACCCTGCAGA GGCAGCCAAG CTTTTCTCCG TGCCTGACTT CGTGGGTGAC 
GCCTGCAAGG CCATTGCATC CCGAGTCCGG GGGGCTGTAG CCTCTGTCAC CTTTGATGAC 
TTCCATAAAA ACTCAGCCCG GATCATTCGA ATGGCTGTTT TTGGCTTTGA GATGTCTGAA 
GACACAGGTC CTGATGGCAC ACTCCTGCCC AAGGCTCGAG ACCAGGCAGT CTTTCCCCAA 
AACGGGCTGG TAGTCAGCAG TGTGGATGTG CAGTCAGTGG AGCCCGTGGA CCAGAGGACC 
CGGGATGCCC TTCAGCGCAG CGTTCAGCTG GCCATCGAAA TTACCACCAA CTCCCAGGAG 
GCAGCAGCCA AGCACGAGGC TCAGAGACTG GAACAGGAAG CCCGTGGTCG GCTTGAGAGG 
CAGAAGATCT TGGACCAGTC AGAAGCTGAA AAAGCCCGCA AGGAACTCTT GGAGCTTGAG 
GCTATGAGCA TGGCTGTGGA GAGCACGGGT AATGCCAAAG CAGAGGCTGA GTCCCGTGCA 
GAGGCAGCGA GGATCGAAGG AGAAGGCTCT GTGCTGCAGG CCAAGCTCAA GGCACAGGCG 
CTAGCCATTG AGACGGAGGC TGAGTTGGAG CGAGTAAAGA AAGTACGAGA GATGGAACTG 
ATCTATGCCC GGGCCCAGTT GGAGCTGGAG GTGAGCAAGG CGCAGCAGCT TGCCAATGTG 
GAGGCAAAGA AGTTCAAGGA GATGACAGAG GCACTGGGCC CCGGCACCAT CAGGGACCTG 
GCTGTGGCCG GGCCAGAGAT GCAGGTGAAA CTTCTCCAGT CCCTGGGCCT GAAATCCACT 
CTCATCACCG ATGGCTCGTC TCCCATCAAC CTCTTCAGCA CAGCCTTCGG GTTGCTGGGG 
CTGGGGTCTG ATGGTCAGCC GCCAGCACAG AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mvp ( NM_022715.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_073206.2 Rattus norvegicus major vault protein (Mvp), mRNA.

Price & Availability↑

CloneID	ORa12977
Clone ID Related Accession (Same CDS sequence)	NM_022715.2
Accession Version	NM_022715.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	major vault protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U09870.2. On Jan 21, 2004 this sequence version replaced NM_022715.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U09870.2, BC071174.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGGCAACTG AAGAGGCCAT CATCCGCATC CCCCCATACC ACTACATCCA TGTGCTGGAC 
CAGAACAGTA ATGTGTCCCG TGTGGAGGTT GGACCAAAGA CCTACATCCG GCAGGACAAT 
GAGAGGGTAC TGTTTGCCCC AGTTCGCATG GTGACCGTCC CCCCACGCCA CTACTGCATA 
GTGGCCAACC CTGTGTCCCG GGACACCCAG AGTTCTGTGT TATTTGACAT CACAGGACAA 
GTCCGACTCC GGCACGCTGA CCAGGAGATC CGACTAGCCC AGGACCCCTT CCCCCTGTAT 
CCAGGGGAGG TGCTGGAAAA GGACATCACC CCACTGCAGG TGGTTCTGCC CAACACAGCA 
CTGCATCTTA AGGCGTTGCT GGACTTTGAG GATAAGAATG GAGACAAGGT CATGGCAGGA 
GACGAGTGGC TATTTGAGGG ACCTGGCACC TACATCCCAC AGAAGGAAGT GGAAGTCGTG 
GAGATCATTC AGGCCACAGT CATCAAACAG AACCAAGCAC TGCGGCTAAG GGCCCGAAAG 
GAGTGCTTTG ACCGGGAGGG CAAGGGGCGC GTGACAGGTG AGGAGTGGCT GGTCCGATCC 
GTGGGGGCTT ACCTCCCAGC TGTCTTTGAA GAGGTGCTGG ATCTGGTGGA TGCTGTGATC 
CTTACAGAAA AGACTGCCCT GCACCTCCGG GCTCTGCAGA ACTTCAGGGA CCTTCGGGGA 
GTGCTCCACC GCACCGGGGA GGAATGGTTA GTGACAGTGC AGGACACAGA AGCCCATGTT 
CCAGATGTCT ATGAGGAGGT GCTTGGGGTA GTACCCATCA CCACCCTGGG ACCTCGACAC 
TACTGTGTCA TTCTTGACCC AATGGGACCA GACGGCAAGA ACCAGCTGGG ACAAAAGCGT 
GTTGTCAAGG GAGAGAAGTC CTTTTTCCTC CAGCCAGGAG AGAGGCTGGA GCGAGGCATC 
CAGGATGTGT ATGTGCTGTC AGAGCAGCAG GGGCTGCTAC TGAAGGCACT GCAGCCCCTG 
GAGGAGGGAG AGAGCGAGGA GAAGGTCTCC CATCAGGCCG GAGACTGCTG GCTCATCCGT 
GGGCCCCTGG AGTATGTGCC ATCTGCAAAA GTGGAGGTGG TGGAGGAGCG TCAGGCTATC 
CCTCTGGACC AAAATGAGGG CATCTATGTG CAGGATGTCA AGACGGGGAA GGTGCGGGCT 
GTGATTGGAA GCACCTACAT GCTGACTCAG GATGAAGTCC TGTGGGAAAA GGAGCTGCCT 
TCTGGGGTGG AGGAGCTGCT GAACTTGGGG CATGACCCTC TGGCAGACAG GGGTCAGAAG 
GGCACAGCCA AGCCCCTTCA GCCCTCAGCT CCAAGGAACA AGACCCGAGT GGTCAGCTAC 
CGTGTCCCGC ACAATGCAGC GGTGCAGGTC TATGACTACA GAGCCAAGAG AGCCCGTGTG 
GTCTTTGGGC CCGAGCTAGT GACACTGGAT CCTGAGGAGC AGTTCACAGT ATTGTCCCTT 
TCTGCCGGGC GACCCAAGCG TCCTCATGCC CGCCGTGCAC TCTGCCTACT GCTGGGACCT 
GATTTCTTTA CTGATGTCAT CACCATCGAA ACTGCAGATC ATGCCAGGTT GCAGCTGCAG 
CTTGCCTACA ACTGGCACTT TGAACTGAAG AACCGGAATG ACCCTGCAGA GGCAGCCAAG 
CTTTTCTCCG TGCCTGACTT CGTGGGTGAC GCCTGCAAGG CCATTGCATC CCGAGTCCGG 
GGGGCTGTAG CCTCTGTCAC CTTTGATGAC TTCCATAAAA ACTCAGCCCG GATCATTCGA 
ATGGCTGTTT TTGGCTTTGA GATGTCTGAA GACACAGGTC CTGATGGCAC ACTCCTGCCC 
AAGGCTCGAG ACCAGGCAGT CTTTCCCCAA AACGGGCTGG TAGTCAGCAG TGTGGATGTG 
CAGTCAGTGG AGCCCGTGGA CCAGAGGACC CGGGATGCCC TTCAGCGCAG CGTTCAGCTG 
GCCATCGAAA TTACCACCAA CTCCCAGGAG GCAGCAGCCA AGCACGAGGC TCAGAGACTG 
GAACAGGAAG CCCGTGGTCG GCTTGAGAGG CAGAAGATCT TGGACCAGTC AGAAGCTGAA 
AAAGCCCGCA AGGAACTCTT GGAGCTTGAG GCTATGAGCA TGGCTGTGGA GAGCACGGGT 
AATGCCAAAG CAGAGGCTGA GTCCCGTGCA GAGGCAGCGA GGATCGAAGG AGAAGGCTCT 
GTGCTGCAGG CCAAGCTCAA GGCACAGGCG CTAGCCATTG AGACGGAGGC TGAGTTGGAG 
CGAGTAAAGA AAGTACGAGA GATGGAACTG ATCTATGCCC GGGCCCAGTT GGAGCTGGAG 
GTGAGCAAGG CGCAGCAGCT TGCCAATGTG GAGGCAAAGA AGTTCAAGGA GATGACAGAG 
GCACTGGGCC CCGGCACCAT CAGGGACCTG GCTGTGGCCG GGCCAGAGAT GCAGGTGAAA 
CTTCTCCAGT CCCTGGGCCT GAAATCCACT CTCATCACCG ATGGCTCGTC TCCCATCAAC 
CTCTTCAGCA CAGCCTTCGG GTTGCTGGGG CTGGGGTCTG ATGGTCAGCC GCCAGCACAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073206.2
CDS	62..2647
Translation	MATEEAIIRIPPYHYIHVLDQNSNVSRVEVGPKTYIRQDNERVLFAPVRMVTVPPRHYCIVANPVSRDTQSSVLFDITGQVRLRHADQEIRLAQDPFPLYPGEVLEKDITPLQVVLPNTALHLKALLDFEDKNGDKVMAGDEWLFEGPGTYIPQKEVEVVEIIQATVIKQNQALRLRARKECFDREGKGRVTGEEWLVRSVGAYLPAVFEEVLDLVDAVILTEKTALHLRALQNFRDLRGVLHRTGEEWLVTVQDTEAHVPDVYEEVLGVVPITTLGPRHYCVILDPMGPDGKNQLGQKRVVKGEKSFFLQPGERLERGIQDVYVLSEQQGLLLKALQPLEEGESEEKVSHQAGDCWLIRGPLEYVPSAKVEVVEERQAIPLDQNEGIYVQDVKTGKVRAVIGSTYMLTQDEVLWEKELPSGVEELLNLGHDPLADRGQKGTAKPLQPSAPRNKTRVVSYRVPHNAAVQVYDYRAKRARVVFGPELVTLDPEEQFTVLSLSAGRPKRPHARRALCLLLGPDFFTDVITIETADHARLQLQLAYNWHFELKNRNDPAEAAKLFSVPDFVGDACKAIASRVRGAVASVTFDDFHKNSARIIRMAVFGFEMSEDTGPDGTLLPKARDQAVFPQNGLVVSSVDVQSVEPVDQRTRDALQRSVQLAIEITTNSQEAAAKHEAQRLEQEARGRLERQKILDQSEAEKARKELLELEAMSMAVESTGNAKAEAESRAEAARIEGEGSVLQAKLKAQALAIETEAELERVKKVREMELIYARAQLELEVSKAQQLANVEAKKFKEMTEALGPGTIRDLAVAGPEMQVKLLQSLGLKSTLITDGSSPINLFSTAFGLLGLGSDGQPPAQK

Target ORF information:

RefSeq Version	NM_022715.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus major vault protein (Mvp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022715.2

ORF Insert Sequence:

ATGGCAACTG AAGAGGCCAT CATCCGCATC CCCCCATACC ACTACATCCA TGTGCTGGAC 
CAGAACAGTA ATGTGTCCCG TGTGGAGGTT GGACCAAAGA CCTACATCCG GCAGGACAAT 
GAGAGGGTAC TGTTTGCCCC AGTTCGCATG GTGACCGTCC CCCCACGCCA CTACTGCATA 
GTGGCCAACC CTGTGTCCCG GGACACCCAG AGTTCTGTGT TATTTGACAT CACAGGACAA 
GTCCGACTCC GGCACGCTGA CCAGGAGATC CGACTAGCCC AGGACCCCTT CCCCCTGTAT 
CCAGGGGAGG TGCTGGAAAA GGACATCACC CCACTGCAGG TGGTTCTGCC CAACACAGCA 
CTGCATCTTA AGGCGTTGCT GGACTTTGAG GATAAGAATG GAGACAAGGT CATGGCAGGA 
GACGAGTGGC TATTTGAGGG ACCTGGCACC TACATCCCAC AGAAGGAAGT GGAAGTCGTG 
GAGATCATTC AGGCCACAGT CATCAAACAG AACCAAGCAC TGCGGCTAAG GGCCCGAAAG 
GAGTGCTTTG ACCGGGAGGG CAAGGGGCGC GTGACAGGTG AGGAGTGGCT GGTCCGATCC 
GTGGGGGCTT ACCTCCCAGC TGTCTTTGAA GAGGTGCTGG ATCTGGTGGA TGCTGTGATC 
CTTACAGAAA AGACTGCCCT GCACCTCCGG GCTCTGCAGA ACTTCAGGGA CCTTCGGGGA 
GTGCTCCACC GCACCGGGGA GGAATGGTTA GTGACAGTGC AGGACACAGA AGCCCATGTT 
CCAGATGTCT ATGAGGAGGT GCTTGGGGTA GTACCCATCA CCACCCTGGG ACCTCGACAC 
TACTGTGTCA TTCTTGACCC AATGGGACCA GACGGCAAGA ACCAGCTGGG ACAAAAGCGT 
GTTGTCAAGG GAGAGAAGTC CTTTTTCCTC CAGCCAGGAG AGAGGCTGGA GCGAGGCATC 
CAGGATGTGT ATGTGCTGTC AGAGCAGCAG GGGCTGCTAC TGAAGGCACT GCAGCCCCTG 
GAGGAGGGAG AGAGCGAGGA GAAGGTCTCC CATCAGGCCG GAGACTGCTG GCTCATCCGT 
GGGCCCCTGG AGTATGTGCC ATCTGCAAAA GTGGAGGTGG TGGAGGAGCG TCAGGCTATC 
CCTCTGGACC AAAATGAGGG CATCTATGTG CAGGATGTCA AGACGGGGAA GGTGCGGGCT 
GTGATTGGAA GCACCTACAT GCTGACTCAG GATGAAGTCC TGTGGGAAAA GGAGCTGCCT 
TCTGGGGTGG AGGAGCTGCT GAACTTGGGG CATGACCCTC TGGCAGACAG GGGTCAGAAG 
GGCACAGCCA AGCCCCTTCA GCCCTCAGCT CCAAGGAACA AGACCCGAGT GGTCAGCTAC 
CGTGTCCCGC ACAATGCAGC GGTGCAGGTC TATGACTACA GAGCCAAGAG AGCCCGTGTG 
GTCTTTGGGC CCGAGCTAGT GACACTGGAT CCTGAGGAGC AGTTCACAGT ATTGTCCCTT 
TCTGCCGGGC GACCCAAGCG TCCTCATGCC CGCCGTGCAC TCTGCCTACT GCTGGGACCT 
GATTTCTTTA CTGATGTCAT CACCATCGAA ACTGCAGATC ATGCCAGGTT GCAGCTGCAG 
CTTGCCTACA ACTGGCACTT TGAACTGAAG AACCGGAATG ACCCTGCAGA GGCAGCCAAG 
CTTTTCTCCG TGCCTGACTT CGTGGGTGAC GCCTGCAAGG CCATTGCATC CCGAGTCCGG 
GGGGCTGTAG CCTCTGTCAC CTTTGATGAC TTCCATAAAA ACTCAGCCCG GATCATTCGA 
ATGGCTGTTT TTGGCTTTGA GATGTCTGAA GACACAGGTC CTGATGGCAC ACTCCTGCCC 
AAGGCTCGAG ACCAGGCAGT CTTTCCCCAA AACGGGCTGG TAGTCAGCAG TGTGGATGTG 
CAGTCAGTGG AGCCCGTGGA CCAGAGGACC CGGGATGCCC TTCAGCGCAG CGTTCAGCTG 
GCCATCGAAA TTACCACCAA CTCCCAGGAG GCAGCAGCCA AGCACGAGGC TCAGAGACTG 
GAACAGGAAG CCCGTGGTCG GCTTGAGAGG CAGAAGATCT TGGACCAGTC AGAAGCTGAA 
AAAGCCCGCA AGGAACTCTT GGAGCTTGAG GCTATGAGCA TGGCTGTGGA GAGCACGGGT 
AATGCCAAAG CAGAGGCTGA GTCCCGTGCA GAGGCAGCGA GGATCGAAGG AGAAGGCTCT 
GTGCTGCAGG CCAAGCTCAA GGCACAGGCG CTAGCCATTG AGACGGAGGC TGAGTTGGAG 
CGAGTAAAGA AAGTACGAGA GATGGAACTG ATCTATGCCC GGGCCCAGTT GGAGCTGGAG 
GTGAGCAAGG CGCAGCAGCT TGCCAATGTG GAGGCAAAGA AGTTCAAGGA GATGACAGAG 
GCACTGGGCC CCGGCACCAT CAGGGACCTG GCTGTGGCCG GGCCAGAGAT GCAGGTGAAA 
CTTCTCCAGT CCCTGGGCCT GAAATCCACT CTCATCACCG ATGGCTCGTC TCCCATCAAC 
CTCTTCAGCA CAGCCTTCGG GTTGCTGGGG CTGGGGTCTG ATGGTCAGCC GCCAGCACAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modulation of the Vault Protein-Protein Interaction for Tuning of Molecular Release.
Scientific reports7(1)14816(2017 Nov)
Yu K,Yau YH,Sinha A,Tan T,Kickhoefer VA,Rome LH,Lee H,Shochat SG,Lim S

Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
Nature communications53650(2014 Apr)
Corominas R,Yang X,Lin GN,Kang S,Shen Y,Ghamsari L,Broly M,Rodriguez M,Tam S,Trigg SA,Fan C,Yi S,Tasan M,Lemmens I,Kuang X,Zhao N,Malhotra D,Michaelson JJ,Vacic V,Calderwood MA,Roth FP,Tavernier J,Horvath S,Salehi-Ashtiani K,Korkin D,Sebat J,Hill DE,Hao T,Vidal M,Iakoucheva LM

Toward an understanding of the protein interaction network of the human liver.
Molecular systems biology7536(2011 Oct)
Wang J,Huo K,Ma L,Tang L,Li D,Huang X,Yuan Y,Li C,Wang W,Guan W,Chen H,Jin C,Wei J,Zhang W,Yang Y,Liu Q,Zhou Y,Zhang C,Wu Z,Xu W,Zhang Y,Liu T,Yu D,Zhang Y,Chen L,Zhu D,Zhong X,Kang L,Gan X,Yu X,Ma Q,Yan J,Zhou L,Liu Z,Zhu Y,Zhou T,He F,Yang X

Protein profile of exosomes from trabecular meshwork cells.
Journal of proteomics74(6)796-804(2011 May)
Stamer WD,Hoffman EA,Luther JM,Hachey DL,Schey KL

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

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Mvp cDNA ORF clone, Rattus norvegicus(Norway rat)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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