Ptk2b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptk2b
Entrez Gene ID	50646
Full Name	protein tyrosine kinase 2 beta
Synonyms	CAK beta,CAKB,CAKbeta,Pyk2
General protein information	Preferred Names protein tyrosine kinase 2 beta Names protein-tyrosine kinase 2-beta CADTK CAK-beta FADK 2 PTK2 protein tyrosine kinase 2 beta PTK2B protein tyrosine kinase 2 beta calcium-dependent tyrosine kinase calcium-regulated non-receptor proline-rich tyrosine kinase cell adhesion kinase beta focal adhesion kinase 2 proline-rich tyrosine kinase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p12
Summary	may phosphorylate focal adhesion kinase FAK; may play a role in actin cytoskeleton reorganization; may mediate thrombin induced astrocyte proliferation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008770774.2	XP_008768996.1	protein-tyrosine kinase 2-beta isoform X1
XM_006252145.3	XP_006252207.1	protein-tyrosine kinase 2-beta isoform X2
NM_017318.2	NP_059014.2	protein-tyrosine kinase 2-beta
XM_006252143.3	XP_006252205.1	protein-tyrosine kinase 2-beta isoform X1
XM_008770773.2	XP_008768995.1	protein-tyrosine kinase 2-beta isoform X1
XM_006252144.3	XP_006252206.1	protein-tyrosine kinase 2-beta isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PTK2B	NP_775267.1
Gallus gallus (chicken)	PTK2B	XP_004935900.1
Pan troglodytes (chimpanzee)	PTK2B	XP_001163381.1
	PTK2B	XP_001109122.1
Bos taurus (cattle)	PTK2B	NP_001095722.1
Mus musculus (house mouse)	Ptk2b	NP_001155838.1
Canis lupus familiaris (dog)	PTK2B	XP_005635736.1
Rattus norvegicus (Norway rat)	Ptk2b	NP_059014.2
Xenopus tropicalis (tropical clawed frog)	ptk2b	NP_001011049.1

Gene Ontology
Bibliography

Related articles in PubMed

Pyk2 inhibition promotes contractile differentiation in arterial smooth muscle.
Grossi M, Bhattachariya A, Nordstr?m I, Turczy?ska KM, Svensson D, Albinsson S, Nilsson BO, Hellstrand P
Journal of cellular physiology232(11)3088-3102(2017 Nov)

The Pyk2/MCU pathway in the rat middle cerebral artery occlusion model of ischemic stroke.
Zhang K, Yan J, Wang L, Tian X, Zhang T, Guo L, Li B, Wang W, Liu X
Neuroscience research(2017 Sep)

OGD induced modification of FAK- and PYK2-coupled pathways in organotypic hippocampal slice cultures.
Ziemka-Nalecz M, Jaworska J, Sypecka J, Zalewska T
Brain research160621-33(2015 May)

A role for the tyrosine kinase Pyk2 in depolarization-induced contraction of vascular smooth muscle.
Mills RD, Mita M, Nakagawa J, Shoji M, Sutherland C, Walsh MP
The Journal of biological chemistry290(14)8677-92(2015 Apr)

Role for NUP62 depletion and PYK2 redistribution in dendritic retraction resulting from chronic stress.
Kinoshita Y, Hunter RG, Gray JD, Mesias R, McEwen BS, Benson DL, Kohtz DS
Proceedings of the National Academy of Sciences of the United States of America111(45)16130-5(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pyk2 inhibition promotes contractile differentiation in carotid artery smooth muscle.
Title: Pyk2 inhibition promotes contractile differentiation in arterial smooth muscle.

Inhibition of the early step of Focal adhesion kinase and proline-rich tyrosine kinase activation may comprise an important portion of the response expressed by modulation of some coupled signal transduction pathways.
Title: OGD induced modification of FAK- and PYK2-coupled pathways in organotypic hippocampal slice cultures.

depolarization-induced entry of Ca(2+) activates Pyk2 upstream of the RhoA/ROK pathway, leading to MYPT1 phosphorylation and MLCP inhibition.
Title: A role for the tyrosine kinase Pyk2 in depolarization-induced contraction of vascular smooth muscle.

Pyk2 is integrated to the PSD-95 signal complex in cortical neurons following Src protein phosphorylation.
Title: The upregulation of NR2A-containing N-methyl-D-aspartate receptor function by tyrosine phosphorylation of postsynaptic density 95 via facilitating Src/proline-rich tyrosine kinase 2 activation.

Role for NUP62 depletion and PYK2 redistribution in dendritic retraction resulting from chronic stress
Title: Role for NUP62 depletion and PYK2 redistribution in dendritic retraction resulting from chronic stress.

The following Ptk2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptk2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa36778	XM_006252145.3 Latest version!	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa13133	NM_017318.2 Latest version!	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
ORa13133	XM_008770773.2 Latest version!	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
ORa13133	XM_006252144.3 Latest version!	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
ORa13133	XM_008770774.2 Latest version!	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
ORa13133	XM_006252143.3 Latest version!	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptk2b ( XM_006252145.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252207.1 Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa36778
Clone ID Related Accession (Same CDS sequence)	XM_006252145.3
Accession Version	XM_006252145.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein-tyrosine kinase 2-beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252145.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGAGGAG 
GACTTCATCC GGCCCAGTAG TCGAGAAGAG GCCCAGCAGC TCTGGGAGGC AGAGAAGATC 
AAGATGAGGC AGGTCCTAGA CAGACAGCAG AAGCAAATGG TGGAAGATTC CCAGTGGCTG 
AGGCGGGAGG AAAGATGCTT GGACCCGATG GTTTATATGA ATGACAAGTC CCCACTGACC 
CCAGAGAAGG AGGCCGGCTA CACGGAGTTC ACAGGGCCCC CCCAGAAGCC ACCACGGCTC 
GGTGCACAGT CCATCCAGCC TACAGCCAAC TTGGACAGGA CTGATGACCT CGTGTACCAC 
AATGTCATGA CCCTGGTGGA GGCCGTGCTG GAACTCAAGA ACAAGCTCAG CCAGCTGCCT 
CCTGAGGAGT ATGTGGTGGT GGTGAAGAAT GTGGGGCTGA ACCTGCGGAA GCTCATCGGT 
AGCGTGGACG ATCTCCTACC CTCCTTGCCA GCATCTTCTA GAACAGAGAT TGAAGGGACC 
CAGAAACTGC TCAACAAAGA CCTGGCAGAG CTCATCAACA AGATGAGGTT GGCTCAGCAG 
AACGCTGTGA CATCCCTAAG TGAGGACTGC AAGCGGCAGA TGCTCACAGC ATCCCATACC 
CTGGCTGTGG ATGCCAAGAA CCTCCTGGAT GCTGTAGACC AAGCCAAGGT TGTGGCTAAT 
CTGGCCCACC CGCCTGCAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252207.1
CDS	224..3127
Translation	MSGVSEPLSRVKVGTLRPPEGPPEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIQEIITSILLSGRIGPNIQLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDTKPTCLAEFKQIRSIRCLPLEETQAVLQLGIEGAPQSLSIKTSSLAEAENMADLIDGYCRLQGEHKGSLIIHAKKDGEKRNSLPQIPTLNLESRRSHLSESCSIESDIYAEIPDETLRRPGGPQYGVAREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWIVMELYPYGELGHYLERNKNSLKVPTLVLYALQICKAMAYLESINCVHRDIAVRNILVASPECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPELCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDIYQMERDIAIEQERNARYRPPKILEPTAFQEPPPKPSRPKYKHPPQTNLLAPKLQFQEEDFIRPSSREEAQQLWEAEKIKMRQVLDRQQKQMVEDSQWLRREERCLDPMVYMNDKSPLTPEKEAGYTEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYHNVMTLVEAVLELKNKLSQLPPEEYVVVVKNVGLNLRKLIGSVDDLLPSLPASSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEDCKRQMLTASHTLAVDAKNLLDAVDQAKVVANLAHPPAE

Target ORF information:

RefSeq Version	XM_006252145.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252145.3

ORF Insert Sequence:

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGAGGAG 
GACTTCATCC GGCCCAGTAG TCGAGAAGAG GCCCAGCAGC TCTGGGAGGC AGAGAAGATC 
AAGATGAGGC AGGTCCTAGA CAGACAGCAG AAGCAAATGG TGGAAGATTC CCAGTGGCTG 
AGGCGGGAGG AAAGATGCTT GGACCCGATG GTTTATATGA ATGACAAGTC CCCACTGACC 
CCAGAGAAGG AGGCCGGCTA CACGGAGTTC ACAGGGCCCC CCCAGAAGCC ACCACGGCTC 
GGTGCACAGT CCATCCAGCC TACAGCCAAC TTGGACAGGA CTGATGACCT CGTGTACCAC 
AATGTCATGA CCCTGGTGGA GGCCGTGCTG GAACTCAAGA ACAAGCTCAG CCAGCTGCCT 
CCTGAGGAGT ATGTGGTGGT GGTGAAGAAT GTGGGGCTGA ACCTGCGGAA GCTCATCGGT 
AGCGTGGACG ATCTCCTACC CTCCTTGCCA GCATCTTCTA GAACAGAGAT TGAAGGGACC 
CAGAAACTGC TCAACAAAGA CCTGGCAGAG CTCATCAACA AGATGAGGTT GGCTCAGCAG 
AACGCTGTGA CATCCCTAAG TGAGGACTGC AAGCGGCAGA TGCTCACAGC ATCCCATACC 
CTGGCTGTGG ATGCCAAGAA CCTCCTGGAT GCTGTAGACC AAGCCAAGGT TGTGGCTAAT 
CTGGCCCACC CGCCTGCAGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptk2b ( NM_017318.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_059014.2 Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), mRNA.

Price & Availability↑

CloneID	ORa13133
Clone ID Related Accession (Same CDS sequence)	XM_008770774.2 , NM_017318.2 , XM_006252143.3 , XM_008770773.2 , XM_006252144.3
Accession Version	NM_017318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	protein-tyrosine kinase 2-beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC101921.1. On Jun 10, 2007 this sequence version replaced NM_017318.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC101921.1, D45854.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGTCCCT 
GAGGGTCTGT GTGCCAGCTC TCCTACACTT ACGAGCCCTA TGGAGTATCC ATCTCCAGTT 
AACTCGCTGC ACACCCCGCC TCTGCACCGA CACAATGTCT TCAAGCGCCA CAGCATGCGG 
GAGGAGGACT TCATCCGGCC CAGTAGTCGA GAAGAGGCCC AGCAGCTCTG GGAGGCAGAG 
AAGATCAAGA TGAGGCAGGT CCTAGACAGA CAGCAGAAGC AAATGGTGGA AGATTCCCAG 
TGGCTGAGGC GGGAGGAAAG ATGCTTGGAC CCGATGGTTT ATATGAATGA CAAGTCCCCA 
CTGACCCCAG AGAAGGAGGC CGGCTACACG GAGTTCACAG GGCCCCCCCA GAAGCCACCA 
CGGCTCGGTG CACAGTCCAT CCAGCCTACA GCCAACTTGG ACAGGACTGA TGACCTCGTG 
TACCACAATG TCATGACCCT GGTGGAGGCC GTGCTGGAAC TCAAGAACAA GCTCAGCCAG 
CTGCCTCCTG AGGAGTATGT GGTGGTGGTG AAGAATGTGG GGCTGAACCT GCGGAAGCTC 
ATCGGTAGCG TGGACGATCT CCTACCCTCC TTGCCAGCAT CTTCTAGAAC AGAGATTGAA 
GGGACCCAGA AACTGCTCAA CAAAGACCTG GCAGAGCTCA TCAACAAGAT GAGGTTGGCT 
CAGCAGAACG CTGTGACATC CCTAAGTGAG GACTGCAAGC GGCAGATGCT CACAGCATCC 
CATACCCTGG CTGTGGATGC CAAGAACCTC CTGGATGCTG TAGACCAAGC CAAGGTTGTG 
GCTAATCTGG CCCACCCGCC TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059014.2
CDS	84..3113
Translation	MSGVSEPLSRVKVGTLRPPEGPPEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIQEIITSILLSGRIGPNIQLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDTKPTCLAEFKQIRSIRCLPLEETQAVLQLGIEGAPQSLSIKTSSLAEAENMADLIDGYCRLQGEHKGSLIIHAKKDGEKRNSLPQIPTLNLESRRSHLSESCSIESDIYAEIPDETLRRPGGPQYGVAREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWIVMELYPYGELGHYLERNKNSLKVPTLVLYALQICKAMAYLESINCVHRDIAVRNILVASPECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPELCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDIYQMERDIAIEQERNARYRPPKILEPTAFQEPPPKPSRPKYKHPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIRPSSREEAQQLWEAEKIKMRQVLDRQQKQMVEDSQWLRREERCLDPMVYMNDKSPLTPEKEAGYTEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYHNVMTLVEAVLELKNKLSQLPPEEYVVVVKNVGLNLRKLIGSVDDLLPSLPASSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEDCKRQMLTASHTLAVDAKNLLDAVDQAKVVANLAHPPAE

Target ORF information:

RefSeq Version	NM_017318.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017318.2

ORF Insert Sequence:

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGTCCCT 
GAGGGTCTGT GTGCCAGCTC TCCTACACTT ACGAGCCCTA TGGAGTATCC ATCTCCAGTT 
AACTCGCTGC ACACCCCGCC TCTGCACCGA CACAATGTCT TCAAGCGCCA CAGCATGCGG 
GAGGAGGACT TCATCCGGCC CAGTAGTCGA GAAGAGGCCC AGCAGCTCTG GGAGGCAGAG 
AAGATCAAGA TGAGGCAGGT CCTAGACAGA CAGCAGAAGC AAATGGTGGA AGATTCCCAG 
TGGCTGAGGC GGGAGGAAAG ATGCTTGGAC CCGATGGTTT ATATGAATGA CAAGTCCCCA 
CTGACCCCAG AGAAGGAGGC CGGCTACACG GAGTTCACAG GGCCCCCCCA GAAGCCACCA 
CGGCTCGGTG CACAGTCCAT CCAGCCTACA GCCAACTTGG ACAGGACTGA TGACCTCGTG 
TACCACAATG TCATGACCCT GGTGGAGGCC GTGCTGGAAC TCAAGAACAA GCTCAGCCAG 
CTGCCTCCTG AGGAGTATGT GGTGGTGGTG AAGAATGTGG GGCTGAACCT GCGGAAGCTC 
ATCGGTAGCG TGGACGATCT CCTACCCTCC TTGCCAGCAT CTTCTAGAAC AGAGATTGAA 
GGGACCCAGA AACTGCTCAA CAAAGACCTG GCAGAGCTCA TCAACAAGAT GAGGTTGGCT 
CAGCAGAACG CTGTGACATC CCTAAGTGAG GACTGCAAGC GGCAGATGCT CACAGCATCC 
CATACCCTGG CTGTGGATGC CAAGAACCTC CTGGATGCTG TAGACCAAGC CAAGGTTGTG 
GCTAATCTGG CCCACCCGCC TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptk2b ( XM_008770774.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768996.1 Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa13133
Clone ID Related Accession (Same CDS sequence)	XM_008770774.2 , NM_017318.2 , XM_006252143.3 , XM_008770773.2 , XM_006252144.3
Accession Version	XM_008770774.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein-tyrosine kinase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGTCCCT 
GAGGGTCTGT GTGCCAGCTC TCCTACACTT ACGAGCCCTA TGGAGTATCC ATCTCCAGTT 
AACTCGCTGC ACACCCCGCC TCTGCACCGA CACAATGTCT TCAAGCGCCA CAGCATGCGG 
GAGGAGGACT TCATCCGGCC CAGTAGTCGA GAAGAGGCCC AGCAGCTCTG GGAGGCAGAG 
AAGATCAAGA TGAGGCAGGT CCTAGACAGA CAGCAGAAGC AAATGGTGGA AGATTCCCAG 
TGGCTGAGGC GGGAGGAAAG ATGCTTGGAC CCGATGGTTT ATATGAATGA CAAGTCCCCA 
CTGACCCCAG AGAAGGAGGC CGGCTACACG GAGTTCACAG GGCCCCCCCA GAAGCCACCA 
CGGCTCGGTG CACAGTCCAT CCAGCCTACA GCCAACTTGG ACAGGACTGA TGACCTCGTG 
TACCACAATG TCATGACCCT GGTGGAGGCC GTGCTGGAAC TCAAGAACAA GCTCAGCCAG 
CTGCCTCCTG AGGAGTATGT GGTGGTGGTG AAGAATGTGG GGCTGAACCT GCGGAAGCTC 
ATCGGTAGCG TGGACGATCT CCTACCCTCC TTGCCAGCAT CTTCTAGAAC AGAGATTGAA 
GGGACCCAGA AACTGCTCAA CAAAGACCTG GCAGAGCTCA TCAACAAGAT GAGGTTGGCT 
CAGCAGAACG CTGTGACATC CCTAAGTGAG GACTGCAAGC GGCAGATGCT CACAGCATCC 
CATACCCTGG CTGTGGATGC CAAGAACCTC CTGGATGCTG TAGACCAAGC CAAGGTTGTG 
GCTAATCTGG CCCACCCGCC TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768996.1
CDS	4922..7951
Translation	MSGVSEPLSRVKVGTLRPPEGPPEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIQEIITSILLSGRIGPNIQLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDTKPTCLAEFKQIRSIRCLPLEETQAVLQLGIEGAPQSLSIKTSSLAEAENMADLIDGYCRLQGEHKGSLIIHAKKDGEKRNSLPQIPTLNLESRRSHLSESCSIESDIYAEIPDETLRRPGGPQYGVAREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWIVMELYPYGELGHYLERNKNSLKVPTLVLYALQICKAMAYLESINCVHRDIAVRNILVASPECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPELCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDIYQMERDIAIEQERNARYRPPKILEPTAFQEPPPKPSRPKYKHPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIRPSSREEAQQLWEAEKIKMRQVLDRQQKQMVEDSQWLRREERCLDPMVYMNDKSPLTPEKEAGYTEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYHNVMTLVEAVLELKNKLSQLPPEEYVVVVKNVGLNLRKLIGSVDDLLPSLPASSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEDCKRQMLTASHTLAVDAKNLLDAVDQAKVVANLAHPPAE

Target ORF information:

RefSeq Version	XM_008770774.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770774.2

ORF Insert Sequence:

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGTCCCT 
GAGGGTCTGT GTGCCAGCTC TCCTACACTT ACGAGCCCTA TGGAGTATCC ATCTCCAGTT 
AACTCGCTGC ACACCCCGCC TCTGCACCGA CACAATGTCT TCAAGCGCCA CAGCATGCGG 
GAGGAGGACT TCATCCGGCC CAGTAGTCGA GAAGAGGCCC AGCAGCTCTG GGAGGCAGAG 
AAGATCAAGA TGAGGCAGGT CCTAGACAGA CAGCAGAAGC AAATGGTGGA AGATTCCCAG 
TGGCTGAGGC GGGAGGAAAG ATGCTTGGAC CCGATGGTTT ATATGAATGA CAAGTCCCCA 
CTGACCCCAG AGAAGGAGGC CGGCTACACG GAGTTCACAG GGCCCCCCCA GAAGCCACCA 
CGGCTCGGTG CACAGTCCAT CCAGCCTACA GCCAACTTGG ACAGGACTGA TGACCTCGTG 
TACCACAATG TCATGACCCT GGTGGAGGCC GTGCTGGAAC TCAAGAACAA GCTCAGCCAG 
CTGCCTCCTG AGGAGTATGT GGTGGTGGTG AAGAATGTGG GGCTGAACCT GCGGAAGCTC 
ATCGGTAGCG TGGACGATCT CCTACCCTCC TTGCCAGCAT CTTCTAGAAC AGAGATTGAA 
GGGACCCAGA AACTGCTCAA CAAAGACCTG GCAGAGCTCA TCAACAAGAT GAGGTTGGCT 
CAGCAGAACG CTGTGACATC CCTAAGTGAG GACTGCAAGC GGCAGATGCT CACAGCATCC 
CATACCCTGG CTGTGGATGC CAAGAACCTC CTGGATGCTG TAGACCAAGC CAAGGTTGTG 
GCTAATCTGG CCCACCCGCC TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptk2b ( XM_006252143.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252205.1 Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa13133
Clone ID Related Accession (Same CDS sequence)	XM_008770774.2 , NM_017318.2 , XM_006252143.3 , XM_008770773.2 , XM_006252144.3
Accession Version	XM_006252143.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein-tyrosine kinase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252143.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGTCCCT 
GAGGGTCTGT GTGCCAGCTC TCCTACACTT ACGAGCCCTA TGGAGTATCC ATCTCCAGTT 
AACTCGCTGC ACACCCCGCC TCTGCACCGA CACAATGTCT TCAAGCGCCA CAGCATGCGG 
GAGGAGGACT TCATCCGGCC CAGTAGTCGA GAAGAGGCCC AGCAGCTCTG GGAGGCAGAG 
AAGATCAAGA TGAGGCAGGT CCTAGACAGA CAGCAGAAGC AAATGGTGGA AGATTCCCAG 
TGGCTGAGGC GGGAGGAAAG ATGCTTGGAC CCGATGGTTT ATATGAATGA CAAGTCCCCA 
CTGACCCCAG AGAAGGAGGC CGGCTACACG GAGTTCACAG GGCCCCCCCA GAAGCCACCA 
CGGCTCGGTG CACAGTCCAT CCAGCCTACA GCCAACTTGG ACAGGACTGA TGACCTCGTG 
TACCACAATG TCATGACCCT GGTGGAGGCC GTGCTGGAAC TCAAGAACAA GCTCAGCCAG 
CTGCCTCCTG AGGAGTATGT GGTGGTGGTG AAGAATGTGG GGCTGAACCT GCGGAAGCTC 
ATCGGTAGCG TGGACGATCT CCTACCCTCC TTGCCAGCAT CTTCTAGAAC AGAGATTGAA 
GGGACCCAGA AACTGCTCAA CAAAGACCTG GCAGAGCTCA TCAACAAGAT GAGGTTGGCT 
CAGCAGAACG CTGTGACATC CCTAAGTGAG GACTGCAAGC GGCAGATGCT CACAGCATCC 
CATACCCTGG CTGTGGATGC CAAGAACCTC CTGGATGCTG TAGACCAAGC CAAGGTTGTG 
GCTAATCTGG CCCACCCGCC TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252205.1
CDS	407..3436
Translation	MSGVSEPLSRVKVGTLRPPEGPPEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIQEIITSILLSGRIGPNIQLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDTKPTCLAEFKQIRSIRCLPLEETQAVLQLGIEGAPQSLSIKTSSLAEAENMADLIDGYCRLQGEHKGSLIIHAKKDGEKRNSLPQIPTLNLESRRSHLSESCSIESDIYAEIPDETLRRPGGPQYGVAREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWIVMELYPYGELGHYLERNKNSLKVPTLVLYALQICKAMAYLESINCVHRDIAVRNILVASPECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPELCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDIYQMERDIAIEQERNARYRPPKILEPTAFQEPPPKPSRPKYKHPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIRPSSREEAQQLWEAEKIKMRQVLDRQQKQMVEDSQWLRREERCLDPMVYMNDKSPLTPEKEAGYTEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYHNVMTLVEAVLELKNKLSQLPPEEYVVVVKNVGLNLRKLIGSVDDLLPSLPASSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEDCKRQMLTASHTLAVDAKNLLDAVDQAKVVANLAHPPAE

Target ORF information:

RefSeq Version	XM_006252143.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252143.3

ORF Insert Sequence:

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGTCCCT 
GAGGGTCTGT GTGCCAGCTC TCCTACACTT ACGAGCCCTA TGGAGTATCC ATCTCCAGTT 
AACTCGCTGC ACACCCCGCC TCTGCACCGA CACAATGTCT TCAAGCGCCA CAGCATGCGG 
GAGGAGGACT TCATCCGGCC CAGTAGTCGA GAAGAGGCCC AGCAGCTCTG GGAGGCAGAG 
AAGATCAAGA TGAGGCAGGT CCTAGACAGA CAGCAGAAGC AAATGGTGGA AGATTCCCAG 
TGGCTGAGGC GGGAGGAAAG ATGCTTGGAC CCGATGGTTT ATATGAATGA CAAGTCCCCA 
CTGACCCCAG AGAAGGAGGC CGGCTACACG GAGTTCACAG GGCCCCCCCA GAAGCCACCA 
CGGCTCGGTG CACAGTCCAT CCAGCCTACA GCCAACTTGG ACAGGACTGA TGACCTCGTG 
TACCACAATG TCATGACCCT GGTGGAGGCC GTGCTGGAAC TCAAGAACAA GCTCAGCCAG 
CTGCCTCCTG AGGAGTATGT GGTGGTGGTG AAGAATGTGG GGCTGAACCT GCGGAAGCTC 
ATCGGTAGCG TGGACGATCT CCTACCCTCC TTGCCAGCAT CTTCTAGAAC AGAGATTGAA 
GGGACCCAGA AACTGCTCAA CAAAGACCTG GCAGAGCTCA TCAACAAGAT GAGGTTGGCT 
CAGCAGAACG CTGTGACATC CCTAAGTGAG GACTGCAAGC GGCAGATGCT CACAGCATCC 
CATACCCTGG CTGTGGATGC CAAGAACCTC CTGGATGCTG TAGACCAAGC CAAGGTTGTG 
GCTAATCTGG CCCACCCGCC TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptk2b ( XM_008770773.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008768995.1 Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X3, mRNA.

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CloneID	ORa13133
Clone ID Related Accession (Same CDS sequence)	XM_008770774.2 , NM_017318.2 , XM_006252143.3 , XM_008770773.2 , XM_006252144.3
Accession Version	XM_008770773.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein-tyrosine kinase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008770773.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGTCCCT 
GAGGGTCTGT GTGCCAGCTC TCCTACACTT ACGAGCCCTA TGGAGTATCC ATCTCCAGTT 
AACTCGCTGC ACACCCCGCC TCTGCACCGA CACAATGTCT TCAAGCGCCA CAGCATGCGG 
GAGGAGGACT TCATCCGGCC CAGTAGTCGA GAAGAGGCCC AGCAGCTCTG GGAGGCAGAG 
AAGATCAAGA TGAGGCAGGT CCTAGACAGA CAGCAGAAGC AAATGGTGGA AGATTCCCAG 
TGGCTGAGGC GGGAGGAAAG ATGCTTGGAC CCGATGGTTT ATATGAATGA CAAGTCCCCA 
CTGACCCCAG AGAAGGAGGC CGGCTACACG GAGTTCACAG GGCCCCCCCA GAAGCCACCA 
CGGCTCGGTG CACAGTCCAT CCAGCCTACA GCCAACTTGG ACAGGACTGA TGACCTCGTG 
TACCACAATG TCATGACCCT GGTGGAGGCC GTGCTGGAAC TCAAGAACAA GCTCAGCCAG 
CTGCCTCCTG AGGAGTATGT GGTGGTGGTG AAGAATGTGG GGCTGAACCT GCGGAAGCTC 
ATCGGTAGCG TGGACGATCT CCTACCCTCC TTGCCAGCAT CTTCTAGAAC AGAGATTGAA 
GGGACCCAGA AACTGCTCAA CAAAGACCTG GCAGAGCTCA TCAACAAGAT GAGGTTGGCT 
CAGCAGAACG CTGTGACATC CCTAAGTGAG GACTGCAAGC GGCAGATGCT CACAGCATCC 
CATACCCTGG CTGTGGATGC CAAGAACCTC CTGGATGCTG TAGACCAAGC CAAGGTTGTG 
GCTAATCTGG CCCACCCGCC TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008768995.1
CDS	1613..4642
Translation	MSGVSEPLSRVKVGTLRPPEGPPEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIQEIITSILLSGRIGPNIQLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDTKPTCLAEFKQIRSIRCLPLEETQAVLQLGIEGAPQSLSIKTSSLAEAENMADLIDGYCRLQGEHKGSLIIHAKKDGEKRNSLPQIPTLNLESRRSHLSESCSIESDIYAEIPDETLRRPGGPQYGVAREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWIVMELYPYGELGHYLERNKNSLKVPTLVLYALQICKAMAYLESINCVHRDIAVRNILVASPECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPELCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDIYQMERDIAIEQERNARYRPPKILEPTAFQEPPPKPSRPKYKHPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIRPSSREEAQQLWEAEKIKMRQVLDRQQKQMVEDSQWLRREERCLDPMVYMNDKSPLTPEKEAGYTEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYHNVMTLVEAVLELKNKLSQLPPEEYVVVVKNVGLNLRKLIGSVDDLLPSLPASSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEDCKRQMLTASHTLAVDAKNLLDAVDQAKVVANLAHPPAE

Target ORF information:

RefSeq Version	XM_008770773.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008770773.2

ORF Insert Sequence:

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGTCCCT 
GAGGGTCTGT GTGCCAGCTC TCCTACACTT ACGAGCCCTA TGGAGTATCC ATCTCCAGTT 
AACTCGCTGC ACACCCCGCC TCTGCACCGA CACAATGTCT TCAAGCGCCA CAGCATGCGG 
GAGGAGGACT TCATCCGGCC CAGTAGTCGA GAAGAGGCCC AGCAGCTCTG GGAGGCAGAG 
AAGATCAAGA TGAGGCAGGT CCTAGACAGA CAGCAGAAGC AAATGGTGGA AGATTCCCAG 
TGGCTGAGGC GGGAGGAAAG ATGCTTGGAC CCGATGGTTT ATATGAATGA CAAGTCCCCA 
CTGACCCCAG AGAAGGAGGC CGGCTACACG GAGTTCACAG GGCCCCCCCA GAAGCCACCA 
CGGCTCGGTG CACAGTCCAT CCAGCCTACA GCCAACTTGG ACAGGACTGA TGACCTCGTG 
TACCACAATG TCATGACCCT GGTGGAGGCC GTGCTGGAAC TCAAGAACAA GCTCAGCCAG 
CTGCCTCCTG AGGAGTATGT GGTGGTGGTG AAGAATGTGG GGCTGAACCT GCGGAAGCTC 
ATCGGTAGCG TGGACGATCT CCTACCCTCC TTGCCAGCAT CTTCTAGAAC AGAGATTGAA 
GGGACCCAGA AACTGCTCAA CAAAGACCTG GCAGAGCTCA TCAACAAGAT GAGGTTGGCT 
CAGCAGAACG CTGTGACATC CCTAAGTGAG GACTGCAAGC GGCAGATGCT CACAGCATCC 
CATACCCTGG CTGTGGATGC CAAGAACCTC CTGGATGCTG TAGACCAAGC CAAGGTTGTG 
GCTAATCTGG CCCACCCGCC TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ptk2b ( XM_006252144.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006252206.1 Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X4, mRNA.

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CloneID	ORa13133
Clone ID Related Accession (Same CDS sequence)	XM_008770774.2 , NM_017318.2 , XM_006252143.3 , XM_008770773.2 , XM_006252144.3
Accession Version	XM_006252144.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein-tyrosine kinase 2-beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006252144.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGTCCCT 
GAGGGTCTGT GTGCCAGCTC TCCTACACTT ACGAGCCCTA TGGAGTATCC ATCTCCAGTT 
AACTCGCTGC ACACCCCGCC TCTGCACCGA CACAATGTCT TCAAGCGCCA CAGCATGCGG 
GAGGAGGACT TCATCCGGCC CAGTAGTCGA GAAGAGGCCC AGCAGCTCTG GGAGGCAGAG 
AAGATCAAGA TGAGGCAGGT CCTAGACAGA CAGCAGAAGC AAATGGTGGA AGATTCCCAG 
TGGCTGAGGC GGGAGGAAAG ATGCTTGGAC CCGATGGTTT ATATGAATGA CAAGTCCCCA 
CTGACCCCAG AGAAGGAGGC CGGCTACACG GAGTTCACAG GGCCCCCCCA GAAGCCACCA 
CGGCTCGGTG CACAGTCCAT CCAGCCTACA GCCAACTTGG ACAGGACTGA TGACCTCGTG 
TACCACAATG TCATGACCCT GGTGGAGGCC GTGCTGGAAC TCAAGAACAA GCTCAGCCAG 
CTGCCTCCTG AGGAGTATGT GGTGGTGGTG AAGAATGTGG GGCTGAACCT GCGGAAGCTC 
ATCGGTAGCG TGGACGATCT CCTACCCTCC TTGCCAGCAT CTTCTAGAAC AGAGATTGAA 
GGGACCCAGA AACTGCTCAA CAAAGACCTG GCAGAGCTCA TCAACAAGAT GAGGTTGGCT 
CAGCAGAACG CTGTGACATC CCTAAGTGAG GACTGCAAGC GGCAGATGCT CACAGCATCC 
CATACCCTGG CTGTGGATGC CAAGAACCTC CTGGATGCTG TAGACCAAGC CAAGGTTGTG 
GCTAATCTGG CCCACCCGCC TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006252206.1
CDS	3397..6426
Translation	MSGVSEPLSRVKVGTLRPPEGPPEPMVVVPVDVEKEDVRILKVCFYSNSFNPGKNFKLVKCTVQTEIQEIITSILLSGRIGPNIQLAECYGLRLKHMKSDEIHWLHPQMTVGEVQDKYECLHVEAEWRYDLQIRYLPEDFMESLKEDRTTLLYFYQQLRNDYMQRYASKVSEGMALQLGCLELRRFFKDMPHNALDKKSNFELLEKEVGLDLFFPKQMQENLKPKQFRKMIQQTFQQYASLREEECVMKFFNTLAGFANIDQETYRCELIQGWNITVDLVIGPKGIRQLTSQDTKPTCLAEFKQIRSIRCLPLEETQAVLQLGIEGAPQSLSIKTSSLAEAENMADLIDGYCRLQGEHKGSLIIHAKKDGEKRNSLPQIPTLNLESRRSHLSESCSIESDIYAEIPDETLRRPGGPQYGVAREDVVLNRILGEGFFGEVYEGVYTNHKGEKINVAVKTCKKDCTLDNKEKFMSEAVIMKNLDHPHIVKLIGIIEEEPTWIVMELYPYGELGHYLERNKNSLKVPTLVLYALQICKAMAYLESINCVHRDIAVRNILVASPECVKLGDFGLSRYIEDEDYYKASVTRLPIKWMSPESINFRRFTTASDVWMFAVCMWEILSFGKQPFFWLENKDVIGVLEKGDRLPKPELCPPVLYTLMTRCWDYDPSDRPRFTELVCSLSDIYQMERDIAIEQERNARYRPPKILEPTAFQEPPPKPSRPKYKHPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIRPSSREEAQQLWEAEKIKMRQVLDRQQKQMVEDSQWLRREERCLDPMVYMNDKSPLTPEKEAGYTEFTGPPQKPPRLGAQSIQPTANLDRTDDLVYHNVMTLVEAVLELKNKLSQLPPEEYVVVVKNVGLNLRKLIGSVDDLLPSLPASSRTEIEGTQKLLNKDLAELINKMRLAQQNAVTSLSEDCKRQMLTASHTLAVDAKNLLDAVDQAKVVANLAHPPAE

Target ORF information:

RefSeq Version	XM_006252144.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein tyrosine kinase 2 beta (Ptk2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006252144.3

ORF Insert Sequence:

ATGTCCGGGG TGTCTGAGCC CCTGAGCCGT GTAAAAGTGG GCACTTTGCG CCCGCCCGAG 
GGCCCCCCAG AGCCCATGGT AGTGGTACCA GTAGACGTGG AGAAGGAAGA CGTGCGTATC 
CTCAAGGTCT GCTTCTACAG CAACAGCTTC AACCCAGGGA AGAACTTCAA GCTTGTCAAA 
TGCACAGTGC AGACAGAGAT CCAGGAGATC ATCACCTCCA TCCTCCTGAG TGGGCGAATA 
GGGCCCAACA TCCAGCTGGC TGAATGCTAT GGGCTGAGGC TGAAGCACAT GAAGTCAGAT 
GAGATCCACT GGCTGCACCC ACAGATGACT GTGGGCGAAG TGCAGGACAA GTATGAATGT 
CTACACGTGG AGGCTGAGTG GAGGTATGAC CTTCAAATCC GCTATTTGCC AGAAGACTTC 
ATGGAGAGCC TGAAAGAAGA CAGGACTACA CTGCTGTACT TTTATCAACA GCTCCGGAAT 
GACTACATGC AACGCTATGC CAGCAAGGTC AGTGAAGGCA TGGCTCTGCA GCTGGGCTGT 
CTGGAGCTCA GGAGATTCTT CAAGGACATG CCCCACAATG CACTTGACAA AAAGTCCAAC 
TTTGAACTCC TGGAAAAAGA AGTCGGTCTG GACCTGTTTT TCCCAAAGCA AATGCAGGAA 
AACTTAAAGC CCAAGCAGTT CCGGAAGATG ATCCAGCAGA CCTTCCAGCA GTATGCATCA 
CTCCGGGAGG AAGAGTGTGT CATGAAATTC TTCAATACTC TGGCAGGCTT TGCCAACATC 
GACCAGGAGA CCTATCGCTG TGAACTCATT CAAGGGTGGA ACATTACTGT GGACCTGGTC 
ATTGGCCCTA AAGGCATCCG TCAGCTGACA AGTCAAGACA CAAAGCCCAC CTGCCTCGCC 
GAGTTCAAGC AGATCAGATC CATCAGGTGC CTCCCCTTGG AAGAGACCCA GGCAGTACTG 
CAGCTGGGCA TCGAGGGTGC CCCCCAGTCC TTGTCCATCA AAACCTCGTC CCTGGCAGAG 
GCTGAGAACA TGGCTGACCT CATAGATGGC TACTGCAGGC TTCAAGGAGA GCATAAAGGC 
TCTCTCATCA TTCATGCCAA GAAAGATGGT GAGAAGAGGA ACAGCCTGCC TCAGATCCCC 
ACACTAAACC TGGAGTCTCG GCGGTCCCAC CTCTCAGAAA GCTGTAGCAT AGAGTCAGAC 
ATCTATGCAG AGATTCCTGA TGAGACCCTG CGAAGGCCAG GAGGCCCACA GTATGGTGTT 
GCCCGTGAAG ATGTAGTTCT TAACCGCATT CTGGGTGAAG GCTTCTTCGG GGAGGTCTAC 
GAAGGTGTCT ACACGAACCA TAAAGGGGAA AAAATTAATG TGGCCGTCAA GACCTGTAAG 
AAAGATTGTA CCCTGGACAA CAAGGAGAAG TTCATGAGTG AGGCAGTGAT CATGAAGAAT 
CTTGACCACC CTCACATCGT CAAGCTGATT GGCATCATTG AAGAGGAACC CACATGGATC 
GTCATGGAAC TGTATCCTTA TGGGGAGCTG GGACACTACC TGGAGCGAAA TAAAAATTCC 
CTGAAGGTAC CCACTCTGGT CCTGTACGCT CTGCAGATAT GCAAAGCCAT GGCCTATCTG 
GAGAGCATCA ACTGTGTCCA CAGGGATATT GCTGTCCGGA ACATCCTGGT GGCCTCTCCT 
GAGTGTGTGA AGCTCGGGGA CTTTGGGCTC TCCCGGTACA TTGAGGATGA GGACTATTAC 
AAAGCTTCCG TGACCCGTCT CCCCATCAAA TGGATGTCCC CCGAGTCCAT CAACTTTCGC 
CGCTTTACAA CCGCCAGTGA CGTCTGGATG TTTGCTGTAT GCATGTGGGA GATCCTCAGC 
TTTGGGAAGC AGCCTTTCTT CTGGCTTGAA AACAAGGATG TCATCGGGGT GCTGGAGAAA 
GGGGACAGGC TGCCTAAGCC CGAACTCTGC CCGCCTGTCC TTTACACACT CATGACCCGC 
TGCTGGGACT ATGACCCCAG CGACCGGCCC CGCTTTACGG AGCTTGTGTG CAGTCTCAGT 
GACATTTATC AGATGGAGAG GGACATTGCA ATAGAACAAG AAAGGAACGC TCGTTACCGA 
CCCCCCAAAA TATTGGAGCC CACTGCTTTT CAGGAACCCC CACCCAAGCC CAGCCGGCCC 
AAGTACAAAC ACCCTCCACA GACCAATCTG CTGGCTCCTA AGCTGCAGTT CCAGGTCCCT 
GAGGGTCTGT GTGCCAGCTC TCCTACACTT ACGAGCCCTA TGGAGTATCC ATCTCCAGTT 
AACTCGCTGC ACACCCCGCC TCTGCACCGA CACAATGTCT TCAAGCGCCA CAGCATGCGG 
GAGGAGGACT TCATCCGGCC CAGTAGTCGA GAAGAGGCCC AGCAGCTCTG GGAGGCAGAG 
AAGATCAAGA TGAGGCAGGT CCTAGACAGA CAGCAGAAGC AAATGGTGGA AGATTCCCAG 
TGGCTGAGGC GGGAGGAAAG ATGCTTGGAC CCGATGGTTT ATATGAATGA CAAGTCCCCA 
CTGACCCCAG AGAAGGAGGC CGGCTACACG GAGTTCACAG GGCCCCCCCA GAAGCCACCA 
CGGCTCGGTG CACAGTCCAT CCAGCCTACA GCCAACTTGG ACAGGACTGA TGACCTCGTG 
TACCACAATG TCATGACCCT GGTGGAGGCC GTGCTGGAAC TCAAGAACAA GCTCAGCCAG 
CTGCCTCCTG AGGAGTATGT GGTGGTGGTG AAGAATGTGG GGCTGAACCT GCGGAAGCTC 
ATCGGTAGCG TGGACGATCT CCTACCCTCC TTGCCAGCAT CTTCTAGAAC AGAGATTGAA 
GGGACCCAGA AACTGCTCAA CAAAGACCTG GCAGAGCTCA TCAACAAGAT GAGGTTGGCT 
CAGCAGAACG CTGTGACATC CCTAAGTGAG GACTGCAAGC GGCAGATGCT CACAGCATCC 
CATACCCTGG CTGTGGATGC CAAGAACCTC CTGGATGCTG TAGACCAAGC CAAGGTTGTG 
GCTAATCTGG CCCACCCGCC TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pyk2 inhibition promotes contractile differentiation in arterial smooth muscle.
Journal of cellular physiology232(11)3088-3102(2017 Nov)
Grossi M,Bhattachariya A,Nordstr?m I,Turczy?ska KM,Svensson D,Albinsson S,Nilsson BO,Hellstrand P

The Pyk2/MCU pathway in the rat middle cerebral artery occlusion model of ischemic stroke.
Neuroscience research(2017 Sep)
Zhang K,Yan J,Wang L,Tian X,Zhang T,Guo L,Li B,Wang W,Liu X

OGD induced modification of FAK- and PYK2-coupled pathways in organotypic hippocampal slice cultures.
Brain research160621-33(2015 May)
Ziemka-Nalecz M,Jaworska J,Sypecka J,Zalewska T

A role for the tyrosine kinase Pyk2 in depolarization-induced contraction of vascular smooth muscle.
The Journal of biological chemistry290(14)8677-92(2015 Apr)
Mills RD,Mita M,Nakagawa J,Shoji M,Sutherland C,Walsh MP

Role for NUP62 depletion and PYK2 redistribution in dendritic retraction resulting from chronic stress.
Proceedings of the National Academy of Sciences of the United States of America111(45)16130-5(2014 Nov)
Kinoshita Y,Hunter RG,Gray JD,Mesias R,McEwen BS,Benson DL,Kohtz DS

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Ptk2b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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