Cert1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cert1
Entrez Gene ID	365652
Full Name	collagen type IV alpha 3 binding protein
General protein information	Preferred Names collagen type IV alpha 3 binding protein Names collagen type IV alpha-3-binding protein alpha 3 type IV collagen binding protein collagen, type IV, alpha 3 (Goodpasture antigen) binding protein procollagen, type IV, alpha 3 (Goodpasture antigen) binding protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231827.3	XP_006231889.1	collagen type IV alpha-3-binding protein isoform X1
NM_001108935.1	NP_001102405.1	collagen type IV alpha-3-binding protein

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	COL4A3BP	XP_424796.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007093	XP_565108.3
Xenopus tropicalis (tropical clawed frog)	col4a3bp	NP_988844.1
Danio rerio (zebrafish)	LOC797430	XP_005161004.1
Drosophila melanogaster (fruit fly)	cert	NP_648199.1
Caenorhabditis elegans (roundworm)	F25H2.6	NP_492762.1
Pan troglodytes (chimpanzee)	COL4A3BP	XP_526893.2
Canis lupus familiaris (dog)	COL4A3BP	XP_849925.2
Homo sapiens (human)	COL4A3BP	NP_001123577.1
	COL4A3BP	XP_002804474.1
Bos taurus (cattle)	COL4A3BP	NP_777190.1
Mus musculus (house mouse)	Col4a3bp	NP_075909.1
Rattus norvegicus (Norway rat)	Col4a3bp	NP_001102405.1

Gene Ontology
Bibliography

Related articles in PubMed

Highly variable penetrance of abnormal phenotypes in embryonic lethal knockout mice.
Wilson R, Geyer SH, Reissig L, Rose J, Szumska D, Hardman E, Prin F, McGuire C, Ramirez-Solis R, White J, Galli A, Tudor C, Tuck E, Mazzeo CI, Smith JC, Robertson E, Adams DJ, Mohun T, Weninger WJ
Wellcome open research11(2016)

Ceramide transfer protein deficiency compromises organelle function and leads to senescence in primary cells.
Rao RP, Scheffer L, Srideshikan SM, Parthibane V, Kosakowska-Cholody T, Masood MA, Nagashima K, Gudla P, Lockett S, Acharya U, Acharya JK
PloS one9(3)e92142(2014)

The expression of the Goodpasture antigen-binding protein (ceramide transporter) in adult rat brain.
Mencarelli C, Hammels C, Van Den Broeck J, Losen M, Steinbusch H, Revert F, Saus J, Hopkins DA, De Baets MH, Steinbusch HW, Martinez-Martinez P
Journal of chemical neuroanatomy38(2)97-105(2009 Oct)

Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice.
Wang X, Rao RP, Kosakowska-Cholody T, Masood MA, Southon E, Zhang H, Berthet C, Nagashim K, Veenstra TK, Tessarollo L, Acharya U, Acharya JK
The Journal of cell biology184(1)143-58(2009 Jan)

Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide.
Kudo N, Kumagai K, Tomishige N, Yamaji T, Wakatsuki S, Nishijima M, Hanada K, Kato R
Proceedings of the National Academy of Sciences of the United States of America105(2)488-93(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Levels of GPBP were observed widely throughout the brain, suggesting that GPBP plays an important role in central nervous system neuron function.
Title: The expression of the Goodpasture antigen-binding protein (ceramide transporter) in adult rat brain.

CERT is a physiological substrate of PP2Cepsilon and that dephosphorylation of CERT by PP2Cepsilon may play an important role in the regulation of ceramide trafficking from the ER to the Golgi apparatus.
Title: Protein phosphatase 2Cepsilon is an endoplasmic reticulum integral membrane protein that dephosphorylates the ceramide transport protein CERT to enhance its association with organelle membranes.

The following Cert1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cert1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa08681	NM_001108935.1 Latest version!	Rattus norvegicus ceramide transporter 1 (Cert1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$370.30 $529.00
ORa24341	XM_006231827.3 Latest version!	Rattus norvegicus collagen type IV alpha 3 binding protein (Col4a3bp), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cert1 ( NM_001108935.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102405.1 Rattus norvegicus ceramide transporter 1 (Cert1), mRNA.

Price & Availability↑

CloneID	ORa08681
Clone ID Related Accession (Same CDS sequence)	NM_001108935.1
Accession Version	NM_001108935.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	collagen type IV alpha-3-binding protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473955.1. On or before Oct 4, 2007 this sequence version replaced XM_001068766.1, XM_345143.3. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGATA ACCAGAGCTG GAACTCGTCG GGCTCGGAGG AGGATCCGGA GACAGAGTCC 
GGGCCGCCTG TGGAGCGCTG CGGAGTCCTC AGCAAGTGGA CAAACTATAT TCATGGGTGG 
CAGGATCGTT GGGTTGTTTT GAAAAATAAT ACTTTGAGTT ACTACAAATC TGAAGATGAA 
ACAGAATATG GCTGTAGGGG ATCCATCTGT CTAAGCAAGG CTGTGATCAC GCCTCATGAC 
TTTGATGAAT GCCGGTTTGA TATTAGTGTG AATGATAGTG TTTGGTATCT TCGTGCTCAG 
GACCCAGATC ATAGACAGCA GTGGGTAGAC GCCATTGAGC AGCACAAGAC TGAATCAGGA 
TATGGATCTG AGTCCAGCTT GCGTAGACAT GGCTCAATGG TGTCACTGGT GTCTGGAGCA 
AGTGGCTATT CTGCTACGTC CACATCTTCA TTCAAGAAAG GCCACAGTTT ACGTGAGAAG 
TTGGCTGAAA TGGAAACCTT TAGAGACATC CTGTGTAGGC AGGTTGATAC TCTGCAGAAG 
TACTTTGATG TCTGTGCTGA TGCTGTCTCC AAGGATGAAC TTCAAAGGGA TAAAGTGGTA 
GAAGACGATG AAGATGACTT CCCTACAACT CGCTCTGATG GAGACTTCTT GCACAATACC 
AATGGTAATA AGGAAAAATT ATTTCCACAT GTAACACCAA AAGGAATTAA TGGCATAGAC 
TTTAAGGGGG AAGCAATAAC TTTTAAAGCA ACTACTGCTG GCATCCTTGC TACACTGTCT 
CATTGTATTG AACTGATGGT AAAACGCGAA GAAAGCTGGC AAAAAAGGCA TGATAGGGAA 
GTGGAAAAGC GGAGACGAGT AGAGGAAGCA TACAAGAATG TGACGGAGGA ACTTAAGAAG 
AAACCTCGCT TCGGAGGGCC AGACTATGAA GAAGGTCCAA ACAGTCTGAT TAATGAGGAA 
GAGTTCTTTG ATGCTGTTGA AGCTGCTCTT GACAGACAAG ATAAAATAGA GGAACAGTCA 
CAGAGTGAAA AGGTCAGGTT ACACTGGCCT CCGTCATTGC CATCTGGAGA TACCTTTTCT 
TCAGTTGGGA CTCACAGATT CGTACAAAAG CCCTATAGTC GCTCTCCCTC CATGTCTTCC 
ATTGATCTAG TCAGTGCCTC TGACGATGTC CACAGATTCA GCTCCCAGGT TGAAGAGATG 
GTACAGAACC ACATGAATTA TTCATTACAG GATGTAGGTG GTGATGCAAA TTGGCAGCTG 
GTTGTAGAAG AAGGAGAAAT GAAGGTGTAC AGAAGAGAAG TTGAAGAAAA TGGAATCGTT 
CTGGATCCTT TGAAAGCTAC TCATGCAGTT AAAGGTGTTA CAGGACACGA GGTCTGCAAT 
TACTTTTGGA ATGTTGATGT TCGCAATGAC TGGGAAACTA CTATAGAAAA CTTTCATGTG 
GTGGAAACAT TAGCTGATAA TGCAATCATC ATTTATCAAA CGCACAAGAG AGTGTGGCCC 
GCTTCTCAGA GAGACGTGCT GTATCTTTCT GCTATTCGGA AGATCCCAGC CTTGACTGAG 
AACGACCCTG AGACTTGGAT AGTTTGTAAT TTCTCTGTGG ACCATGACAG TGCTCCTCTG 
AACAATCGAT GTGTCCGTGC CAAAATCAAT ATTGCTTTGA TTTGTCAAAC TTTGGTAAGC 
CCACCAGAGG GAGACCAGGA GATAAGCAGA GACAACATTC TGTGCAAGAT TACATATGTA 
GCTAATGTGA ACCCAGGAGG ATGGGCGCCA GCCTCGGTGT TAAGAGCAGT GGCAAAGCGA 
GAATACCCTA AGTTTCTAAA ACGATTTACT TCTTATGTCC AAGAAAAAAC TGCAGGAAAA 
CCAATTTTGT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102405.1
CDS	425..2299
Translation	MSDNQSWNSSGSEEDPETESGPPVERCGVLSKWTNYIHGWQDRWVVLKNNTLSYYKSEDETEYGCRGSICLSKAVITPHDFDECRFDISVNDSVWYLRAQDPDHRQQWVDAIEQHKTESGYGSESSLRRHGSMVSLVSGASGYSATSTSSFKKGHSLREKLAEMETFRDILCRQVDTLQKYFDVCADAVSKDELQRDKVVEDDEDDFPTTRSDGDFLHNTNGNKEKLFPHVTPKGINGIDFKGEAITFKATTAGILATLSHCIELMVKREESWQKRHDREVEKRRRVEEAYKNVTEELKKKPRFGGPDYEEGPNSLINEEEFFDAVEAALDRQDKIEEQSQSEKVRLHWPPSLPSGDTFSSVGTHRFVQKPYSRSPSMSSIDLVSASDDVHRFSSQVEEMVQNHMNYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLADNAIIIYQTHKRVWPASQRDVLYLSAIRKIPALTENDPETWIVCNFSVDHDSAPLNNRCVRAKINIALICQTLVSPPEGDQEISRDNILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF

Target ORF information:

RefSeq Version	NM_001108935.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ceramide transporter 1 (Cert1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108935.1

ORF Insert Sequence:

ATGTCGGATA ACCAGAGCTG GAACTCGTCG GGCTCGGAGG AGGATCCGGA GACAGAGTCC 
GGGCCGCCTG TGGAGCGCTG CGGAGTCCTC AGCAAGTGGA CAAACTATAT TCATGGGTGG 
CAGGATCGTT GGGTTGTTTT GAAAAATAAT ACTTTGAGTT ACTACAAATC TGAAGATGAA 
ACAGAATATG GCTGTAGGGG ATCCATCTGT CTAAGCAAGG CTGTGATCAC GCCTCATGAC 
TTTGATGAAT GCCGGTTTGA TATTAGTGTG AATGATAGTG TTTGGTATCT TCGTGCTCAG 
GACCCAGATC ATAGACAGCA GTGGGTAGAC GCCATTGAGC AGCACAAGAC TGAATCAGGA 
TATGGATCTG AGTCCAGCTT GCGTAGACAT GGCTCAATGG TGTCACTGGT GTCTGGAGCA 
AGTGGCTATT CTGCTACGTC CACATCTTCA TTCAAGAAAG GCCACAGTTT ACGTGAGAAG 
TTGGCTGAAA TGGAAACCTT TAGAGACATC CTGTGTAGGC AGGTTGATAC TCTGCAGAAG 
TACTTTGATG TCTGTGCTGA TGCTGTCTCC AAGGATGAAC TTCAAAGGGA TAAAGTGGTA 
GAAGACGATG AAGATGACTT CCCTACAACT CGCTCTGATG GAGACTTCTT GCACAATACC 
AATGGTAATA AGGAAAAATT ATTTCCACAT GTAACACCAA AAGGAATTAA TGGCATAGAC 
TTTAAGGGGG AAGCAATAAC TTTTAAAGCA ACTACTGCTG GCATCCTTGC TACACTGTCT 
CATTGTATTG AACTGATGGT AAAACGCGAA GAAAGCTGGC AAAAAAGGCA TGATAGGGAA 
GTGGAAAAGC GGAGACGAGT AGAGGAAGCA TACAAGAATG TGACGGAGGA ACTTAAGAAG 
AAACCTCGCT TCGGAGGGCC AGACTATGAA GAAGGTCCAA ACAGTCTGAT TAATGAGGAA 
GAGTTCTTTG ATGCTGTTGA AGCTGCTCTT GACAGACAAG ATAAAATAGA GGAACAGTCA 
CAGAGTGAAA AGGTCAGGTT ACACTGGCCT CCGTCATTGC CATCTGGAGA TACCTTTTCT 
TCAGTTGGGA CTCACAGATT CGTACAAAAG CCCTATAGTC GCTCTCCCTC CATGTCTTCC 
ATTGATCTAG TCAGTGCCTC TGACGATGTC CACAGATTCA GCTCCCAGGT TGAAGAGATG 
GTACAGAACC ACATGAATTA TTCATTACAG GATGTAGGTG GTGATGCAAA TTGGCAGCTG 
GTTGTAGAAG AAGGAGAAAT GAAGGTGTAC AGAAGAGAAG TTGAAGAAAA TGGAATCGTT 
CTGGATCCTT TGAAAGCTAC TCATGCAGTT AAAGGTGTTA CAGGACACGA GGTCTGCAAT 
TACTTTTGGA ATGTTGATGT TCGCAATGAC TGGGAAACTA CTATAGAAAA CTTTCATGTG 
GTGGAAACAT TAGCTGATAA TGCAATCATC ATTTATCAAA CGCACAAGAG AGTGTGGCCC 
GCTTCTCAGA GAGACGTGCT GTATCTTTCT GCTATTCGGA AGATCCCAGC CTTGACTGAG 
AACGACCCTG AGACTTGGAT AGTTTGTAAT TTCTCTGTGG ACCATGACAG TGCTCCTCTG 
AACAATCGAT GTGTCCGTGC CAAAATCAAT ATTGCTTTGA TTTGTCAAAC TTTGGTAAGC 
CCACCAGAGG GAGACCAGGA GATAAGCAGA GACAACATTC TGTGCAAGAT TACATATGTA 
GCTAATGTGA ACCCAGGAGG ATGGGCGCCA GCCTCGGTGT TAAGAGCAGT GGCAAAGCGA 
GAATACCCTA AGTTTCTAAA ACGATTTACT TCTTATGTCC AAGAAAAAAC TGCAGGAAAA 
CCAATTTTGT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cert1 ( XM_006231827.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231889.1 Rattus norvegicus collagen type IV alpha 3 binding protein (Col4a3bp), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24341
Clone ID Related Accession (Same CDS sequence)	XM_006231827.3
Accession Version	XM_006231827.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen type IV alpha-3-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231827.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGATA ACCAGAGCTG GAACTCGTCG GGCTCGGAGG AGGATCCGGA GACAGAGTCC 
GGGCCGCCTG TGGAGCGCTG CGGAGTCCTC AGCAAGTGGA CAAACTATAT TCATGGGTGG 
CAGGATCGTT GGGTTGTTTT GAAAAATAAT ACTTTGAGTT ACTACAAATC TGAAGATGAA 
ACAGAATATG GCTGTAGGGG ATCCATCTGT CTAAGCAAGG CTGTGATCAC GCCTCATGAC 
TTTGATGAAT GCCGGTTTGA TATTAGTGTG AATGATAGTG TTTGGTATCT TCGTGCTCAG 
GACCCAGATC ATAGACAGCA GTGGGTAGAC GCCATTGAGC AGCACAAGAC TGAATCAGGA 
TATGGATCTG AGTCCAGCTT GCGTAGACAT GGCTCAATGG TGTCACTGGT GTCTGGAGCA 
AGTGGCTATT CTGCTACGTC CACATCTTCA TTCAAGAAAG GCCACAGTTT ACGTGAGAAG 
TTGGCTGAAA TGGAAACCTT TAGAGACATC CTGTGTAGGC AGGTTGATAC TCTGCAGAAG 
TACTTTGATG TCTGTGCTGA TGCTGTCTCC AAGGATGAAC TTCAAAGGGA TAAAGTGGTA 
GAAGACGATG AAGATGACTT CCCTACAACT CGCTCTGATG GAGACTTCTT GCACAATACC 
AATGGTAATA AGGAAAAATT ATTTCCACAT GTAACACCAA AAGGAATTAA TGGCATAGAC 
TTTAAGGGGG AAGCAATAAC TTTTAAAGCA ACTACTGCTG GCATCCTTGC TACACTGTCT 
CATTGTATTG AACTGATGGT AAAACGCGAA GAAAGCTGGC AAAAAAGGCA TGATAGGGAA 
GTGGAAAAGC GGAGACGAGT AGAGGAAGCA TACAAGAATG TGACGGAGGA ACTTAAGAAG 
AAACCTCGCT TCGGAGGGCC AGACTATGAA GAAGGTCCAA ACAGTCTGAT TAATGAGGAA 
GAGTTCTTTG ATGCTGTTGA AGCTGCTCTT GACAGACAAG ATAAAATAGA GGAACAGTCA 
CAGAGTGAAA AGGTCAGGTT ACACTGGCCT CCGTCATTGC CATCTGGAGA TACCTTTTCT 
TCAGTTGGGA CTCACAGATT CGTACAAAAG GTTGAAGAGA TGGTACAGAA CCACATGAAT 
TATTCATTAC AGGATGTAGG TGGTGATGCA AATTGGCAGC TGGTTGTAGA AGAAGGAGAA 
ATGAAGGTGT ACAGAAGAGA AGTTGAAGAA AATGGAATCG TTCTGGATCC TTTGAAAGCT 
ACTCATGCAG TTAAAGGTGT TACAGGACAC GAGGTCTGCA ATTACTTTTG GAATGTTGAT 
GTTCGCAATG ACTGGGAAAC TACTATAGAA AACTTTCATG TGGTGGAAAC ATTAGCTGAT 
AATGCAATCA TCATTTATCA AACGCACAAG AGAGTGTGGC CCGCTTCTCA GAGAGACGTG 
CTGTATCTTT CTGCTATTCG GAAGATCCCA GCCTTGACTG AGAACGACCC TGAGACTTGG 
ATAGTTTGTA ATTTCTCTGT GGACCATGAC AGTGCTCCTC TGAACAATCG ATGTGTCCGT 
GCCAAAATCA ATATTGCTTT GATTTGTCAA ACTTTGGTAA GCCCACCAGA GGGAGACCAG 
GAGATAAGCA GAGACAACAT TCTGTGCAAG ATTACATATG TAGCTAATGT GAACCCAGGA 
GGATGGGCGC CAGCCTCGGT GTTAAGAGCA GTGGCAAAGC GAGAATACCC TAAGTTTCTA 
AAACGATTTA CTTCTTATGT CCAAGAAAAA ACTGCAGGAA AACCAATTTT GTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231889.1
CDS	428..2224
Translation	MSDNQSWNSSGSEEDPETESGPPVERCGVLSKWTNYIHGWQDRWVVLKNNTLSYYKSEDETEYGCRGSICLSKAVITPHDFDECRFDISVNDSVWYLRAQDPDHRQQWVDAIEQHKTESGYGSESSLRRHGSMVSLVSGASGYSATSTSSFKKGHSLREKLAEMETFRDILCRQVDTLQKYFDVCADAVSKDELQRDKVVEDDEDDFPTTRSDGDFLHNTNGNKEKLFPHVTPKGINGIDFKGEAITFKATTAGILATLSHCIELMVKREESWQKRHDREVEKRRRVEEAYKNVTEELKKKPRFGGPDYEEGPNSLINEEEFFDAVEAALDRQDKIEEQSQSEKVRLHWPPSLPSGDTFSSVGTHRFVQKVEEMVQNHMNYSLQDVGGDANWQLVVEEGEMKVYRREVEENGIVLDPLKATHAVKGVTGHEVCNYFWNVDVRNDWETTIENFHVVETLADNAIIIYQTHKRVWPASQRDVLYLSAIRKIPALTENDPETWIVCNFSVDHDSAPLNNRCVRAKINIALICQTLVSPPEGDQEISRDNILCKITYVANVNPGGWAPASVLRAVAKREYPKFLKRFTSYVQEKTAGKPILF

Target ORF information:

RefSeq Version	XM_006231827.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type IV alpha 3 binding protein (Col4a3bp), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231827.3

ORF Insert Sequence:

ATGTCGGATA ACCAGAGCTG GAACTCGTCG GGCTCGGAGG AGGATCCGGA GACAGAGTCC 
GGGCCGCCTG TGGAGCGCTG CGGAGTCCTC AGCAAGTGGA CAAACTATAT TCATGGGTGG 
CAGGATCGTT GGGTTGTTTT GAAAAATAAT ACTTTGAGTT ACTACAAATC TGAAGATGAA 
ACAGAATATG GCTGTAGGGG ATCCATCTGT CTAAGCAAGG CTGTGATCAC GCCTCATGAC 
TTTGATGAAT GCCGGTTTGA TATTAGTGTG AATGATAGTG TTTGGTATCT TCGTGCTCAG 
GACCCAGATC ATAGACAGCA GTGGGTAGAC GCCATTGAGC AGCACAAGAC TGAATCAGGA 
TATGGATCTG AGTCCAGCTT GCGTAGACAT GGCTCAATGG TGTCACTGGT GTCTGGAGCA 
AGTGGCTATT CTGCTACGTC CACATCTTCA TTCAAGAAAG GCCACAGTTT ACGTGAGAAG 
TTGGCTGAAA TGGAAACCTT TAGAGACATC CTGTGTAGGC AGGTTGATAC TCTGCAGAAG 
TACTTTGATG TCTGTGCTGA TGCTGTCTCC AAGGATGAAC TTCAAAGGGA TAAAGTGGTA 
GAAGACGATG AAGATGACTT CCCTACAACT CGCTCTGATG GAGACTTCTT GCACAATACC 
AATGGTAATA AGGAAAAATT ATTTCCACAT GTAACACCAA AAGGAATTAA TGGCATAGAC 
TTTAAGGGGG AAGCAATAAC TTTTAAAGCA ACTACTGCTG GCATCCTTGC TACACTGTCT 
CATTGTATTG AACTGATGGT AAAACGCGAA GAAAGCTGGC AAAAAAGGCA TGATAGGGAA 
GTGGAAAAGC GGAGACGAGT AGAGGAAGCA TACAAGAATG TGACGGAGGA ACTTAAGAAG 
AAACCTCGCT TCGGAGGGCC AGACTATGAA GAAGGTCCAA ACAGTCTGAT TAATGAGGAA 
GAGTTCTTTG ATGCTGTTGA AGCTGCTCTT GACAGACAAG ATAAAATAGA GGAACAGTCA 
CAGAGTGAAA AGGTCAGGTT ACACTGGCCT CCGTCATTGC CATCTGGAGA TACCTTTTCT 
TCAGTTGGGA CTCACAGATT CGTACAAAAG GTTGAAGAGA TGGTACAGAA CCACATGAAT 
TATTCATTAC AGGATGTAGG TGGTGATGCA AATTGGCAGC TGGTTGTAGA AGAAGGAGAA 
ATGAAGGTGT ACAGAAGAGA AGTTGAAGAA AATGGAATCG TTCTGGATCC TTTGAAAGCT 
ACTCATGCAG TTAAAGGTGT TACAGGACAC GAGGTCTGCA ATTACTTTTG GAATGTTGAT 
GTTCGCAATG ACTGGGAAAC TACTATAGAA AACTTTCATG TGGTGGAAAC ATTAGCTGAT 
AATGCAATCA TCATTTATCA AACGCACAAG AGAGTGTGGC CCGCTTCTCA GAGAGACGTG 
CTGTATCTTT CTGCTATTCG GAAGATCCCA GCCTTGACTG AGAACGACCC TGAGACTTGG 
ATAGTTTGTA ATTTCTCTGT GGACCATGAC AGTGCTCCTC TGAACAATCG ATGTGTCCGT 
GCCAAAATCA ATATTGCTTT GATTTGTCAA ACTTTGGTAA GCCCACCAGA GGGAGACCAG 
GAGATAAGCA GAGACAACAT TCTGTGCAAG ATTACATATG TAGCTAATGT GAACCCAGGA 
GGATGGGCGC CAGCCTCGGT GTTAAGAGCA GTGGCAAAGC GAGAATACCC TAAGTTTCTA 
AAACGATTTA CTTCTTATGT CCAAGAAAAA ACTGCAGGAA AACCAATTTT GTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Highly variable penetrance of abnormal phenotypes in embryonic lethal knockout mice.
Wellcome open research11(2016)
Wilson R,Geyer SH,Reissig L,Rose J,Szumska D,Hardman E,Prin F,McGuire C,Ramirez-Solis R,White J,Galli A,Tudor C,Tuck E,Mazzeo CI,Smith JC,Robertson E,Adams DJ,Mohun T,Weninger WJ

Ceramide transfer protein deficiency compromises organelle function and leads to senescence in primary cells.
PloS one9(3)e92142(2014)
Rao RP,Scheffer L,Srideshikan SM,Parthibane V,Kosakowska-Cholody T,Masood MA,Nagashima K,Gudla P,Lockett S,Acharya U,Acharya JK

The expression of the Goodpasture antigen-binding protein (ceramide transporter) in adult rat brain.
Journal of chemical neuroanatomy38(2)97-105(2009 Oct)
Mencarelli C,Hammels C,Van Den Broeck J,Losen M,Steinbusch H,Revert F,Saus J,Hopkins DA,De Baets MH,Steinbusch HW,Martinez-Martinez P

Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice.
The Journal of cell biology184(1)143-58(2009 Jan)
Wang X,Rao RP,Kosakowska-Cholody T,Masood MA,Southon E,Zhang H,Berthet C,Nagashim K,Veenstra TK,Tessarollo L,Acharya U,Acharya JK

Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide.
Proceedings of the National Academy of Sciences of the United States of America105(2)488-93(2008 Jan)
Kudo N,Kumagai K,Tomishige N,Yamaji T,Wakatsuki S,Nishijima M,Hanada K,Kato R

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Cert1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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