C5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	C5
Entrez Gene ID	362119
Full Name	complement C5
Synonyms	C5a,Hc,RGD1561905
General protein information	Preferred Names complement C5 Names complement C5 anaphylatoxin complement component 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p11

mRNA and Protein(s)

mRNA	Protein	Name
XM_003749455.4	XP_003749503.2	complement C5 isoform X2
NM_053020.1	NP_444179.1	complement C5 precursor
XM_001079130.6	XP_001079130.2	complement C5 isoform X3
XM_008761798.1	XP_008760020.1	complement C5 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Role of Complement C5 in Experimental Blunt Chest Trauma-Induced Septic Acute Lung Injury (ALI).
Kalbitz M, Karbach M, Braumueller S, Kellermann P, Gebhard F, Huber-Lang M, Perl M
PloS one11(7)e0159417(2016)

Renal C3 complement component: feed forward to diabetic kidney disease.
Kelly KJ, Liu Y, Zhang J, Dominguez JH
American journal of nephrology41(1)48-56(2015)

Complement C5-deficient mice are protected from seizures in experimental cerebral malaria.
Buckingham SC, Ramos TN, Barnum SR
Epilepsia55(12)e139-42(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

n the complex but clinically relevant DH model the local and systemic inflammatory immune response features both, C5-dependent and C5-independent characteristics. Activation of caspase-3 in lung tissue after DH was C5-dependent whereas local inflammation in lung tissue was C5-independent.
Title: Role of Complement C5 in Experimental Blunt Chest Trauma-Induced Septic Acute Lung Injury (ALI).

Th data of this study suggested a role for the Complement C5 in malaria-induced seizures and that inhibition of the terminal complement pathway may reduce seizures and seizure-related neurocognitive deficits.
Title: Complement C5-deficient mice are protected from seizures in experimental cerebral malaria.

data indicate that the mechanism of IL-6 and TGF-beta1 production in renal tissue of Thy-1N rats is associated with sublytic C5b-9 up-regulated p300 and p300-mediated C/EBPbeta acetylation
Title: Sublytic C5b-9 induces IL-6 and TGF-?1 production by glomerular mesangial cells in rat Thy-1 nephritis through p300-mediated C/EBP? acetylation.

Data indicate that absence of complement C3, complement C5 and Fc receptor subunit gamma (FcRgamma) does not impact autoantibody production in K/BxN mice.
Title: Autoantibody-mediated arthritis in the absence of C3 and activating Fc? receptors: C5 is activated by the coagulation cascade.

Early immune-inflammatory response to blast injury involves complement and TNFalpha, which correlates with hippocampus and cerebral cortex damage.
Title: Blast-induced moderate neurotrauma (BINT) elicits early complement activation and tumor necrosis factor ? (TNF?) release in a rat brain.

The following C5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the C5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa25764	XM_003749455.4 Latest version!	Rattus norvegicus complement component 5 (C5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa48131	XM_008761798.1 Latest version!	Rattus norvegicus complement component 5 (C5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa15554	NM_053020.1 Latest version!	Rattus norvegicus complement C5 (C5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ORa15554	XM_001079130.6 Latest version!	Rattus norvegicus complement component 5 (C5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

C5 ( XM_003749455.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_003749503.2 Rattus norvegicus complement component 5 (C5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa25764
Clone ID Related Accession (Same CDS sequence)	XM_003749455.4
Accession Version	XM_003749455.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	complement C5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003749455.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCTTC CAGCCATGGG CCTTTGGGGA CTACTTTGCC TTTTAATTTT CCTGGACAAG 
ACCTGGGGAC AGGAACAAAC CTACGTCATT TCAGCACCCA AAATCTTCCG GGTCGGATCA 
TCCGAAAATG TCGTAATTCA AGCCCATGGC TACACCGAAG CCTTTGACGC AACGATCTCT 
CTGAAAAGCT ATCCTGACAA AAAAGTGACC TACTCTTCCG GCTATGTTAA CTTGTCGCCG 
GAAAACAAAT TCCAGAACTC AGCACTGTTG ACATTACCGC CCAAACAATT TCCCAGAGAT 
GAAAACCCAG TCTCTCACGT GTATCTGGAA GTTGTGTCAA TGCACTTTTC GAAATCGAAG 
AAAATACCAA TAACCTATGA CAATGGATTT CTCTTCATCC ATACAGACAA ACCTGTTTAC 
ACTCCAGACC AGTCAGTAAA GATTAGAGTC TACTCTCTGA GTGACGACTT GAAGCCAGCC 
AAACGGGAGA CTGTCTTAAC TTTCGTAGAT CCCGAAGGAA CAGAAGTTGA CATCGTAGAA 
GAAAATGATT ATACTGGAAT TATCTCTTTT CCTGACTTCA AGATTCCATC TAATCCCAAG 
TATGGTGTAT GGACAATTAA AGCTAAATAT AAGAAGGATT TTACAACGAC TGGAACTGCA 
TACTTTGAAG TTAAAGAATA TGTCTTGCCC CGATTCTCTG TATCAATAGA ACCAGAAAGC 
AACTTCATTG GCTATAAGAA CTTTAAGAAC TTTGAAATCA CTGTGAAAGC AAGATATTTT 
TATAATAAAA TGGTCCCCGA TGCTGAAGTC TATATCTTTT TTGGGTTGAG AGAGGACATA 
AAAGAGGATG AGAAACAGAT GATGCATAAA GCCATGCAAG CCGCAACGGT AAGATATTTG 
CTCAGATGTA ATGAGCATGA GTTACGTGCA AACCCTCAGT GCAGTCCCAC ATGCTGGACA 
CCTGCGGACA GTGCAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003749503.2
CDS	60..1040
Translation	MPLPAMGLWGLLCLLIFLDKTWGQEQTYVISAPKIFRVGSSENVVIQAHGYTEAFDATISLKSYPDKKVTYSSGYVNLSPENKFQNSALLTLPPKQFPRDENPVSHVYLEVVSMHFSKSKKIPITYDNGFLFIHTDKPVYTPDQSVKIRVYSLSDDLKPAKRETVLTFVDPEGTEVDIVEENDYTGIISFPDFKIPSNPKYGVWTIKAKYKKDFTTTGTAYFEVKEYVLPRFSVSIEPESNFIGYKNFKNFEITVKARYFYNKMVPDAEVYIFFGLREDIKEDEKQMMHKAMQAATVRYLLRCNEHELRANPQCSPTCWTPADSAA

Target ORF information:

RefSeq Version	XM_003749455.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus complement component 5 (C5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003749455.4

ORF Insert Sequence:

ATGCCGCTTC CAGCCATGGG CCTTTGGGGA CTACTTTGCC TTTTAATTTT CCTGGACAAG 
ACCTGGGGAC AGGAACAAAC CTACGTCATT TCAGCACCCA AAATCTTCCG GGTCGGATCA 
TCCGAAAATG TCGTAATTCA AGCCCATGGC TACACCGAAG CCTTTGACGC AACGATCTCT 
CTGAAAAGCT ATCCTGACAA AAAAGTGACC TACTCTTCCG GCTATGTTAA CTTGTCGCCG 
GAAAACAAAT TCCAGAACTC AGCACTGTTG ACATTACCGC CCAAACAATT TCCCAGAGAT 
GAAAACCCAG TCTCTCACGT GTATCTGGAA GTTGTGTCAA TGCACTTTTC GAAATCGAAG 
AAAATACCAA TAACCTATGA CAATGGATTT CTCTTCATCC ATACAGACAA ACCTGTTTAC 
ACTCCAGACC AGTCAGTAAA GATTAGAGTC TACTCTCTGA GTGACGACTT GAAGCCAGCC 
AAACGGGAGA CTGTCTTAAC TTTCGTAGAT CCCGAAGGAA CAGAAGTTGA CATCGTAGAA 
GAAAATGATT ATACTGGAAT TATCTCTTTT CCTGACTTCA AGATTCCATC TAATCCCAAG 
TATGGTGTAT GGACAATTAA AGCTAAATAT AAGAAGGATT TTACAACGAC TGGAACTGCA 
TACTTTGAAG TTAAAGAATA TGTCTTGCCC CGATTCTCTG TATCAATAGA ACCAGAAAGC 
AACTTCATTG GCTATAAGAA CTTTAAGAAC TTTGAAATCA CTGTGAAAGC AAGATATTTT 
TATAATAAAA TGGTCCCCGA TGCTGAAGTC TATATCTTTT TTGGGTTGAG AGAGGACATA 
AAAGAGGATG AGAAACAGAT GATGCATAAA GCCATGCAAG CCGCAACGGT AAGATATTTG 
CTCAGATGTA ATGAGCATGA GTTACGTGCA AACCCTCAGT GCAGTCCCAC ATGCTGGACA 
CCTGCGGACA GTGCAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C5 ( XM_008761798.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760020.1 Rattus norvegicus complement component 5 (C5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa48131
Clone ID Related Accession (Same CDS sequence)	XM_008761798.1
Accession Version	XM_008761798.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	complement C5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAA CAGTCAATGT GAATCAAGAG ACATCTGACT TGGAACCAAA GAGGAGCATC 
ACACACTCTG CTGATGGAGT GGCTTCATTT GTGGTGAACC TCCCATCAGA AGTGACATCA 
CTGAAGTTTG AGGTCAAAAC TGATGCCCCG GAACTTCCCG AAGAAAATCA AGCCAGCAAA 
GAATATGAAG CAGTTACATA CTCATCCCTC AGCCAGAGTT ACATTTACAT TGGCTGGACT 
GAAAACTACA AGCCCATGCT TGTGGGAGAA TATCTGAATA TTATCGTCAC CCCCAAGAGT 
CCATATATTG ACAAAATAAC TCACTATAAT TACTTGATTT TATCCAAAGG CAAAATTGTA 
CAGTATGGCA CAAAGGAGAA ACTTCTCTAT TCATCTTATC AAAATATAAA CATCCCAGTG 
ACACAGGACA TGGTTCCTTC AGCGCGGCTC CTGGTCTATT ACATAGTCAC GGGGGAGCAG 
ACAGCAGAAT TGGTGGCTGA CGCAGTCTGG ATAAACATTG AGGAGAAGTG TGGCAACCAG 
CTCCAGGTCC ATCTGTCTCC AGATAAAGAC GTGTATTCTC CAGGCCAAAC TGTGTCCCTT 
GACATGGTGA CTGAAGCAGA CTCATGGGTG GCACTATCTG CGGTGGACAG CGCTGTGTAT 
GGAGTCCGGG GAAAAGCCAA AAGGGCCATG CAAAGAGTGT TCCAAGCTTT TGATGACAAG 
AGTGACCTGG GCTGTGGGGC AGGTGGTGGC CGTGACAATG TAGATGTATT CCATCTAGCT 
GGGCTCACCT TCCTCACCAA TGCAAACGCA GATGACTCCC AATACCACGA TGACTCTTGT 
AAGGAAATTC TCAGGCCAAA GAGAGACCTG CAGCTCCTGC ATCAGAAAGT GGAAGAACAA 
GCTGCTAAAT ACAAACACCG TGTGCCCAAG AAATGCTGTT ATGATGGAGC CCGAGAAAAC 
AAATACGAAA CCTGTGAGCA GCGAGTTGCC CGGGTGACCA TAGGCCCACA CTGCATCAGG 
GCCTTCAACG AGTGTTGTAC TATTGCGGAT AAGATCCGAA AAGAAAGCCA CCACAAAGGC 
ATGCTGTTGG GAAGGATCCA AATAAAGGCC CTGTTACCAG TGATGAAGGC AGAAATCCGA 
AGCTACTTTC CAGAGAGCTG GCTATGGGAA GTTCATCGTG TTCCCAAAAG AAACCAGCTG 
CAGGTTGCAC TGCCTGACTC ACTGACGACC TGGGAAATTC AAGGCATCGG CATCTCAGAC 
AATGGTATAT GTGTTGCTGA CACACTCAAG GCAAAGGTGT TCAAAGATGT CTTCCTGGAG 
ATGAACATAC CATATTCTGT TGTACGAGGG GAGCAGATCC AATTGAAGGG AACCGTTTAC 
AATTATAGGA CCTCTGGGAC AATGTTCTGT GTTAAAATGT CTGCCGTGGA GGGAATCTGC 
ACTCCAGGAA GCTCGGCTGC TAGCCCTCAG ACCTCTAGGT CCTCCAGATG TGTGCGCCAG 
AGAATAGAGG GCTCCTCCAG TCACTTGGTG ACCTTCAGCC TGCTTCCTCT GGAAATTGGC 
CTTCACTCCA TAAACTTCTC ACTAGAGACT TCATTTGGGA AAGAAATCTT AGTGAAGACA 
TTACGGGTAG TGCCAGAAGG GATCAAAAGG GAAAGCTATG CTGGTGTGAC TCTGGACCCC 
AGGGGAGTTT ATGGTATTGT TAACAGACGA AAGGAATTCC CATACAGGAT ACCATTAGAT 
TTGGTCCCCA AAACCAACGT CAAAAGGATT TTGAGTGTAA AAGGACTGCT TATAGGGGAA 
TTCTTGTCCA CGGTTCTGAG TAAAGAAGGC ATCGACATCC TAACCCACCT CCCCAAGGGC 
AGCGCCGAGG CAGAACTCAT GAGCATAGTC CCGGTGTTCT ACGTTTTCCA CTACCTGGAA 
GCAGGAAACC ATTGGAATAT TTTCCACCCT GATACGTTAG CTAGAAAACA GAGCCTGCAG 
AAAAAAATAA AAGAAGGGCT GGTGAGCGTC ATGTCCTACA GAAACGCTGA CTATTCCTAC 
AGCATGTGGA AGGGAGCAAG CTCTAGTGCC TGGCTGACAG CTTTTGCTCT GAGAGTGCTT 
GGACAGGTGA ACAAGTATGT GAAACAAGAC CAATACTCGA TCTGTAACTC CTTGTTATGG 
CTGATTGAGA AGTGTCAGCT GGAAAACGGA TCTTTCAAGG AAAATTCCCA ATATCTACCA 
ATAAAATTAC AGGGTACTTT GCCTGCTGAA GCCCAAGAGA ACACTTTATA TCTTACAGCC 
TTTTCTGTGA TTGGAATTAG AAAGGCTATT GGCATATGCC CCACGGAGAA AATCTACACA 
GCGCTGGCTA AAGCTGACTC CTTCCTACTT GAAAGGACCC TGCCTTCCAA GAGCACCTTC 
ACCCTGGCCA TTGTGGCCTA TGCTCTCTCC CTGGGAGACA GAACCCACCC GAAGTTTCGT 
TCTATTGTGT CAGCCCTGAA GAGGGAAGCT TTGGTTAAAG GAGACCCGCC CATTTACCGT 
TTCTGGAGAG ACACTCTCCA ACGTCCAGAC AGCTCAGCAC CCAACAGCGG CACAGCAGGT 
ATGGTAGAAA CCACGGCCTA TGCTTTGCTC ACCAGCCTGA ACCTGAAGGA GACGAGTTAT 
GTCAACCCGA TCATCAAGTG GCTATCTGAG GAGCAGAGGT ATGGAGGCGG CTTTTATTCC 
ACCCAGGATA CCATTAACGC CATCGAGGGC CTGACAGAGT ATTCACTCCT GGTTAAACAA 
CTTCATTTGG ATATGGATAT CAATGTCTCC TACAAACACA AAGGGGATTT CTACCAGTAT 
AAAGTGACAG AGAAGAACTT CCTCGGGAGG CCAGTGGAGG TACCCCTCAA TGATGACCTC 
ATCGTCACCA CAGGCTATAG CAGTGGCTTG GCTACAGTAT ATGTAAAAAC TGTGGTTCAC 
AAAACTAGTG TCGCTGAGGA ATTTTGCAGC TTTTACTTGA AAATTGATAC CCAAGAAGTT 
GAAGCCTCCA GCTACCTCAG CTACAGTGAC TCGGGACACA AGCGCATAAT AGCCTGTGCC 
AGCTACAAGC CCAGCAAGGA GGAGTCAGCA TCTGGGTCCT CCCATGCAGT AATGGATATA 
CTGCTGCCGA CCGGAATCGG AGCAAACCAA GAAGATTTAC GAGCTCTTGT GGAAGGAGTA 
GATCAACTCC TAACTGATTA CCAGATCAAA GACAGTCATG TTATTCTGCA ATTGAATTCG 
ATTCCCTCCA GAGATTTCCT TTGTGTTCGG TTCCGGATAT TTGAACTTTT CCAAGTTGGG 
TTTCTGAATC CTGCTACGTT CACGGTGTAC GAGTATCACA GACCAGATAA GCAGTGTACC 
ATGATTTACA GCACTTCTGA CACCAACCTT CAGAGAGTCT GTGAAGGAGC GGCATGCAAA 
TGCGTTGAAG CTGATTGTGG GCAACTGCAG GCAGAACTGG ACCTGGCCAT CTCTGCAGAC 
ACCAGGAAAG AAACAGCATG TAAACCAGAG ATTGCATATG CTTATAAGGT CAGGATCACG 
TCGGCCACGG AAGAAAACAT TTTTGTCAAG TACACTGCGA CGCTTCTGGA TATTTACAAA 
ACAGGGGAAG CCGCTGCTGA GAAGGACTCT GAGATCACCT TCATTAAAAA GATAAGCTGT 
ACCAACGCCA ACCTGGTGAA AGGAAAGCAA TATTTAATCA TGGGCAAAGA GGCTCTGCAG 
ATCAAACACA ATTTCAGTTT CAAGTATATA TACCCTCTAG ATTCCTCCAC CTGGATTGAA 
TATTGGCCCA CAGACACAAC GTGTCCATCC TGCCAAGCGT TTGTAGCTAA TTTGGACGAG 
TTCGCTGAAG ACATCTTTCT AAATGGCTGT GAAAATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760020.1
CDS	208..4089
Translation	MAQTVNVNQETSDLEPKRSITHSADGVASFVVNLPSEVTSLKFEVKTDAPELPEENQASKEYEAVTYSSLSQSYIYIGWTENYKPMLVGEYLNIIVTPKSPYIDKITHYNYLILSKGKIVQYGTKEKLLYSSYQNINIPVTQDMVPSARLLVYYIVTGEQTAELVADAVWINIEEKCGNQLQVHLSPDKDVYSPGQTVSLDMVTEADSWVALSAVDSAVYGVRGKAKRAMQRVFQAFDDKSDLGCGAGGGRDNVDVFHLAGLTFLTNANADDSQYHDDSCKEILRPKRDLQLLHQKVEEQAAKYKHRVPKKCCYDGARENKYETCEQRVARVTIGPHCIRAFNECCTIADKIRKESHHKGMLLGRIQIKALLPVMKAEIRSYFPESWLWEVHRVPKRNQLQVALPDSLTTWEIQGIGISDNGICVADTLKAKVFKDVFLEMNIPYSVVRGEQIQLKGTVYNYRTSGTMFCVKMSAVEGICTPGSSAASPQTSRSSRCVRQRIEGSSSHLVTFSLLPLEIGLHSINFSLETSFGKEILVKTLRVVPEGIKRESYAGVTLDPRGVYGIVNRRKEFPYRIPLDLVPKTNVKRILSVKGLLIGEFLSTVLSKEGIDILTHLPKGSAEAELMSIVPVFYVFHYLEAGNHWNIFHPDTLARKQSLQKKIKEGLVSVMSYRNADYSYSMWKGASSSAWLTAFALRVLGQVNKYVKQDQYSICNSLLWLIEKCQLENGSFKENSQYLPIKLQGTLPAEAQENTLYLTAFSVIGIRKAIGICPTEKIYTALAKADSFLLERTLPSKSTFTLAIVAYALSLGDRTHPKFRSIVSALKREALVKGDPPIYRFWRDTLQRPDSSAPNSGTAGMVETTAYALLTSLNLKETSYVNPIIKWLSEEQRYGGGFYSTQDTINAIEGLTEYSLLVKQLHLDMDINVSYKHKGDFYQYKVTEKNFLGRPVEVPLNDDLIVTTGYSSGLATVYVKTVVHKTSVAEEFCSFYLKIDTQEVEASSYLSYSDSGHKRIIACASYKPSKEESASGSSHAVMDILLPTGIGANQEDLRALVEGVDQLLTDYQIKDSHVILQLNSIPSRDFLCVRFRIFELFQVGFLNPATFTVYEYHRPDKQCTMIYSTSDTNLQRVCEGAACKCVEADCGQLQAELDLAISADTRKETACKPEIAYAYKVRITSATEENIFVKYTATLLDIYKTGEAAAEKDSEITFIKKISCTNANLVKGKQYLIMGKEALQIKHNFSFKYIYPLDSSTWIEYWPTDTTCPSCQAFVANLDEFAEDIFLNGCENA

Target ORF information:

RefSeq Version	XM_008761798.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus complement component 5 (C5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761798.1

ORF Insert Sequence:

ATGGCACAAA CAGTCAATGT GAATCAAGAG ACATCTGACT TGGAACCAAA GAGGAGCATC 
ACACACTCTG CTGATGGAGT GGCTTCATTT GTGGTGAACC TCCCATCAGA AGTGACATCA 
CTGAAGTTTG AGGTCAAAAC TGATGCCCCG GAACTTCCCG AAGAAAATCA AGCCAGCAAA 
GAATATGAAG CAGTTACATA CTCATCCCTC AGCCAGAGTT ACATTTACAT TGGCTGGACT 
GAAAACTACA AGCCCATGCT TGTGGGAGAA TATCTGAATA TTATCGTCAC CCCCAAGAGT 
CCATATATTG ACAAAATAAC TCACTATAAT TACTTGATTT TATCCAAAGG CAAAATTGTA 
CAGTATGGCA CAAAGGAGAA ACTTCTCTAT TCATCTTATC AAAATATAAA CATCCCAGTG 
ACACAGGACA TGGTTCCTTC AGCGCGGCTC CTGGTCTATT ACATAGTCAC GGGGGAGCAG 
ACAGCAGAAT TGGTGGCTGA CGCAGTCTGG ATAAACATTG AGGAGAAGTG TGGCAACCAG 
CTCCAGGTCC ATCTGTCTCC AGATAAAGAC GTGTATTCTC CAGGCCAAAC TGTGTCCCTT 
GACATGGTGA CTGAAGCAGA CTCATGGGTG GCACTATCTG CGGTGGACAG CGCTGTGTAT 
GGAGTCCGGG GAAAAGCCAA AAGGGCCATG CAAAGAGTGT TCCAAGCTTT TGATGACAAG 
AGTGACCTGG GCTGTGGGGC AGGTGGTGGC CGTGACAATG TAGATGTATT CCATCTAGCT 
GGGCTCACCT TCCTCACCAA TGCAAACGCA GATGACTCCC AATACCACGA TGACTCTTGT 
AAGGAAATTC TCAGGCCAAA GAGAGACCTG CAGCTCCTGC ATCAGAAAGT GGAAGAACAA 
GCTGCTAAAT ACAAACACCG TGTGCCCAAG AAATGCTGTT ATGATGGAGC CCGAGAAAAC 
AAATACGAAA CCTGTGAGCA GCGAGTTGCC CGGGTGACCA TAGGCCCACA CTGCATCAGG 
GCCTTCAACG AGTGTTGTAC TATTGCGGAT AAGATCCGAA AAGAAAGCCA CCACAAAGGC 
ATGCTGTTGG GAAGGATCCA AATAAAGGCC CTGTTACCAG TGATGAAGGC AGAAATCCGA 
AGCTACTTTC CAGAGAGCTG GCTATGGGAA GTTCATCGTG TTCCCAAAAG AAACCAGCTG 
CAGGTTGCAC TGCCTGACTC ACTGACGACC TGGGAAATTC AAGGCATCGG CATCTCAGAC 
AATGGTATAT GTGTTGCTGA CACACTCAAG GCAAAGGTGT TCAAAGATGT CTTCCTGGAG 
ATGAACATAC CATATTCTGT TGTACGAGGG GAGCAGATCC AATTGAAGGG AACCGTTTAC 
AATTATAGGA CCTCTGGGAC AATGTTCTGT GTTAAAATGT CTGCCGTGGA GGGAATCTGC 
ACTCCAGGAA GCTCGGCTGC TAGCCCTCAG ACCTCTAGGT CCTCCAGATG TGTGCGCCAG 
AGAATAGAGG GCTCCTCCAG TCACTTGGTG ACCTTCAGCC TGCTTCCTCT GGAAATTGGC 
CTTCACTCCA TAAACTTCTC ACTAGAGACT TCATTTGGGA AAGAAATCTT AGTGAAGACA 
TTACGGGTAG TGCCAGAAGG GATCAAAAGG GAAAGCTATG CTGGTGTGAC TCTGGACCCC 
AGGGGAGTTT ATGGTATTGT TAACAGACGA AAGGAATTCC CATACAGGAT ACCATTAGAT 
TTGGTCCCCA AAACCAACGT CAAAAGGATT TTGAGTGTAA AAGGACTGCT TATAGGGGAA 
TTCTTGTCCA CGGTTCTGAG TAAAGAAGGC ATCGACATCC TAACCCACCT CCCCAAGGGC 
AGCGCCGAGG CAGAACTCAT GAGCATAGTC CCGGTGTTCT ACGTTTTCCA CTACCTGGAA 
GCAGGAAACC ATTGGAATAT TTTCCACCCT GATACGTTAG CTAGAAAACA GAGCCTGCAG 
AAAAAAATAA AAGAAGGGCT GGTGAGCGTC ATGTCCTACA GAAACGCTGA CTATTCCTAC 
AGCATGTGGA AGGGAGCAAG CTCTAGTGCC TGGCTGACAG CTTTTGCTCT GAGAGTGCTT 
GGACAGGTGA ACAAGTATGT GAAACAAGAC CAATACTCGA TCTGTAACTC CTTGTTATGG 
CTGATTGAGA AGTGTCAGCT GGAAAACGGA TCTTTCAAGG AAAATTCCCA ATATCTACCA 
ATAAAATTAC AGGGTACTTT GCCTGCTGAA GCCCAAGAGA ACACTTTATA TCTTACAGCC 
TTTTCTGTGA TTGGAATTAG AAAGGCTATT GGCATATGCC CCACGGAGAA AATCTACACA 
GCGCTGGCTA AAGCTGACTC CTTCCTACTT GAAAGGACCC TGCCTTCCAA GAGCACCTTC 
ACCCTGGCCA TTGTGGCCTA TGCTCTCTCC CTGGGAGACA GAACCCACCC GAAGTTTCGT 
TCTATTGTGT CAGCCCTGAA GAGGGAAGCT TTGGTTAAAG GAGACCCGCC CATTTACCGT 
TTCTGGAGAG ACACTCTCCA ACGTCCAGAC AGCTCAGCAC CCAACAGCGG CACAGCAGGT 
ATGGTAGAAA CCACGGCCTA TGCTTTGCTC ACCAGCCTGA ACCTGAAGGA GACGAGTTAT 
GTCAACCCGA TCATCAAGTG GCTATCTGAG GAGCAGAGGT ATGGAGGCGG CTTTTATTCC 
ACCCAGGATA CCATTAACGC CATCGAGGGC CTGACAGAGT ATTCACTCCT GGTTAAACAA 
CTTCATTTGG ATATGGATAT CAATGTCTCC TACAAACACA AAGGGGATTT CTACCAGTAT 
AAAGTGACAG AGAAGAACTT CCTCGGGAGG CCAGTGGAGG TACCCCTCAA TGATGACCTC 
ATCGTCACCA CAGGCTATAG CAGTGGCTTG GCTACAGTAT ATGTAAAAAC TGTGGTTCAC 
AAAACTAGTG TCGCTGAGGA ATTTTGCAGC TTTTACTTGA AAATTGATAC CCAAGAAGTT 
GAAGCCTCCA GCTACCTCAG CTACAGTGAC TCGGGACACA AGCGCATAAT AGCCTGTGCC 
AGCTACAAGC CCAGCAAGGA GGAGTCAGCA TCTGGGTCCT CCCATGCAGT AATGGATATA 
CTGCTGCCGA CCGGAATCGG AGCAAACCAA GAAGATTTAC GAGCTCTTGT GGAAGGAGTA 
GATCAACTCC TAACTGATTA CCAGATCAAA GACAGTCATG TTATTCTGCA ATTGAATTCG 
ATTCCCTCCA GAGATTTCCT TTGTGTTCGG TTCCGGATAT TTGAACTTTT CCAAGTTGGG 
TTTCTGAATC CTGCTACGTT CACGGTGTAC GAGTATCACA GACCAGATAA GCAGTGTACC 
ATGATTTACA GCACTTCTGA CACCAACCTT CAGAGAGTCT GTGAAGGAGC GGCATGCAAA 
TGCGTTGAAG CTGATTGTGG GCAACTGCAG GCAGAACTGG ACCTGGCCAT CTCTGCAGAC 
ACCAGGAAAG AAACAGCATG TAAACCAGAG ATTGCATATG CTTATAAGGT CAGGATCACG 
TCGGCCACGG AAGAAAACAT TTTTGTCAAG TACACTGCGA CGCTTCTGGA TATTTACAAA 
ACAGGGGAAG CCGCTGCTGA GAAGGACTCT GAGATCACCT TCATTAAAAA GATAAGCTGT 
ACCAACGCCA ACCTGGTGAA AGGAAAGCAA TATTTAATCA TGGGCAAAGA GGCTCTGCAG 
ATCAAACACA ATTTCAGTTT CAAGTATATA TACCCTCTAG ATTCCTCCAC CTGGATTGAA 
TATTGGCCCA CAGACACAAC GTGTCCATCC TGCCAAGCGT TTGTAGCTAA TTTGGACGAG 
TTCGCTGAAG ACATCTTTCT AAATGGCTGT GAAAATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C5 ( NM_053020.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_444179.1 Rattus norvegicus complement C5 (C5), mRNA.

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CloneID	ORa15554
Clone ID Related Accession (Same CDS sequence)	NM_053020.1 , XM_001079130.6
Accession Version	NM_053020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Rattus norvegicus(Norway rat)
Product	complement C5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01020893.1, AAHX01020892.1, AAHX01020890.1, AAHX01020889.1 and AAHX01020888.1. On Dec 6, 2019 this sequence version replaced XM_001079130.6. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGCTTC CAGCCATGGG CCTTTGGGGA CTACTTTGCC TTTTAATTTT CCTGGACAAG 
ACCTGGGGAC AGGAACAAAC CTACGTCATT TCAGCACCCA AAATCTTCCG GGTCGGATCA 
TCCGAAAATG TCGTAATTCA AGCCCATGGC TACACCGAAG CCTTTGACGC AACGATCTCT 
CTGAAAAGCT ATCCTGACAA AAAAGTGACC TACTCTTCCG GCTATGTTAA CTTGTCGCCG 
GAAAACAAAT TCCAGAACTC AGCACTGTTG ACATTACCGC CCAAACAATT TCCCAGAGAT 
GAAAACCCAG TCTCTCACGT GTATCTGGAA GTTGTGTCAA TGCACTTTTC GAAATCGAAG 
AAAATACCAA TAACCTATGA CAATGGATTT CTCTTCATCC ATACAGACAA ACCTGTTTAC 
ACTCCAGACC AGTCAGTAAA GATTAGAGTC TACTCTCTGA GTGACGACTT GAAGCCAGCC 
AAACGGGAGA CTGTCTTAAC TTTCGTAGAT CCCGAAGGAA CAGAAGTTGA CATCGTAGAA 
GAAAATGATT ATACTGGAAT TATCTCTTTT CCTGACTTCA AGATTCCATC TAATCCCAAG 
TATGGTGTAT GGACAATTAA AGCTAAATAT AAGAAGGATT TTACAACGAC TGGAACTGCA 
TACTTTGAAG TTAAAGAATA TGTCTTGCCC CGATTCTCTG TATCAATAGA ACCAGAAAGC 
AACTTCATTG GCTATAAGAA CTTTAAGAAC TTTGAAATCA CTGTGAAAGC AAGATATTTT 
TATAATAAAA TGGTCCCCGA TGCTGAAGTC TATATCTTTT TTGGGTTGAG AGAGGACATA 
AAAGAGGATG AGAAACAGAT GATGCATAAA GCCATGCAAG CCGCAACGTT GGTCAATGGA 
GTTGCTCAGA TCTCTTTTGA CTCTGAAACA GCAGTGAAAG AGCTGTCATA TGAAAGTCTA 
GAAGATCTAA ACAATAAGTA CCTTTATATT GCTGTAACAG TCACAGAGTC TTCAGGTGGG 
TTTTCGGAAG AGGCAGAAAT TCCTGGCATC AAATACGTCC TCTCTCCCTA TACACTGAAT 
TTGGTCGCTA CCCCTCTTTT CCTGAAGCCT GGGATTCCAT TTTCCATCAA GGTACAGGTT 
AAGGATTCAC TCGAGCAGTT GGTAGGAGGG GTCCCAGTAA CTCTGATGGC ACAAACAGTC 
AATGTGAATC AAGAGACATC TGACTTGGAA CCAAAGAGGA GCATCACACA CTCTGCTGAT 
GGAGTGGCTT CATTTGTGGT GAACCTCCCA TCAGAAGTGA CATCACTGAA GTTTGAGGTC 
AAAACTGATG CCCCGGAACT TCCCGAAGAA AATCAAGCCA GCAAAGAATA TGAAGCAGTT 
ACATACTCAT CCCTCAGCCA GAGTTACATT TACATTGGCT GGACTGAAAA CTACAAGCCC 
ATGCTTGTGG GAGAATATCT GAATATTATC GTCACCCCCA AGAGTCCATA TATTGACAAA 
ATAACTCACT ATAATTACTT GATTTTATCC AAAGGCAAAA TTGTACAGTA TGGCACAAAG 
GAGAAACTTC TCTATTCATC TTATCAAAAT ATAAACATCC CAGTGACACA GGACATGGTT 
CCTTCAGCGC GGCTCCTGGT CTATTACATA GTCACGGGGG AGCAGACAGC AGAATTGGTG 
GCTGACGCAG TCTGGATAAA CATTGAGGAG AAGTGTGGCA ACCAGCTCCA GGTCCATCTG 
TCTCCAGATA AAGACGTGTA TTCTCCAGGC CAAACTGTGT CCCTTGACAT GGTGACTGAA 
GCAGACTCAT GGGTGGCACT ATCTGCGGTG GACAGCGCTG TGTATGGAGT CCGGGGAAAA 
GCCAAAAGGG CCATGCAAAG AGTGTTCCAA GCTTTTGATG ACAAGAGTGA CCTGGGCTGT 
GGGGCAGGTG GTGGCCGTGA CAATGTAGAT GTATTCCATC TAGCTGGGCT CACCTTCCTC 
ACCAATGCAA ACGCAGATGA CTCCCAATAC CACGATGACT CTTGTAAGGA AATTCTCAGG 
CCAAAGAGAG ACCTGCAGCT CCTGCATCAG AAAGTGGAAG AACAAGCTGC TAAATACAAA 
CACCGTGTGC CCAAGAAATG CTGTTATGAT GGAGCCCGAG AAAACAAATA CGAAACCTGT 
GAGCAGCGAG TTGCCCGGGT GACCATAGGC CCACACTGCA TCAGGGCCTT CAACGAGTGT 
TGTACTATTG CGGATAAGAT CCGAAAAGAA AGCCACCACA AAGGCATGCT GTTGGGAAGG 
ATCCAAATAA AGGCCCTGTT ACCAGTGATG AAGGCAGAAA TCCGAAGCTA CTTTCCAGAG 
AGCTGGCTAT GGGAAGTTCA TCGTGTTCCC AAAAGAAACC AGCTGCAGGT TGCACTGCCT 
GACTCACTGA CGACCTGGGA AATTCAAGGC ATCGGCATCT CAGACAATGG TATATGTGTT 
GCTGACACAC TCAAGGCAAA GGTGTTCAAA GATGTCTTCC TGGAGATGAA CATACCATAT 
TCTGTTGTAC GAGGGGAGCA GATCCAATTG AAGGGAACCG TTTACAATTA TAGGACCTCT 
GGGACAATGT TCTGTGTTAA AATGTCTGCC GTGGAGGGAA TCTGCACTCC AGGAAGCTCG 
GCTGCTAGCC CTCAGACCTC TAGGTCCTCC AGATGTGTGC GCCAGAGAAT AGAGGGCTCC 
TCCAGTCACT TGGTGACCTT CAGCCTGCTT CCTCTGGAAA TTGGCCTTCA CTCCATAAAC 
TTCTCACTAG AGACTTCATT TGGGAAAGAA ATCTTAGTGA AGACATTACG GGTAGTGCCA 
GAAGGGATCA AAAGGGAAAG CTATGCTGGT GTGACTCTGG ACCCCAGGGG AGTTTATGGT 
ATTGTTAACA GACGAAAGGA ATTCCCATAC AGGATACCAT TAGATTTGGT CCCCAAAACC 
AACGTCAAAA GGATTTTGAG TGTAAAAGGA CTGCTTATAG GGGAATTCTT GTCCACGGTT 
CTGAGTAAAG AAGGCATCGA CATCCTAACC CACCTCCCCA AGGGCAGCGC CGAGGCAGAA 
CTCATGAGCA TAGTCCCGGT GTTCTACGTT TTCCACTACC TGGAAGCAGG AAACCATTGG 
AATATTTTCC ACCCTGATAC GTTAGCTAGA AAACAGAGCC TGCAGAAAAA AATAAAAGAA 
GGGCTGGTGA GCGTCATGTC CTACAGAAAC GCTGACTATT CCTACAGCAT GTGGAAGGGA 
GCAAGCTCTA GTGCCTGGCT GACAGCTTTT GCTCTGAGAG TGCTTGGACA GGTGAACAAG 
TATGTGAAAC AAGACCAATA CTCGATCTGT AACTCCTTGT TATGGCTGAT TGAGAAGTGT 
CAGCTGGAAA ACGGATCTTT CAAGGAAAAT TCCCAATATC TACCAATAAA ATTACAGGGT 
ACTTTGCCTG CTGAAGCCCA AGAGAACACT TTATATCTTA CAGCCTTTTC TGTGATTGGA 
ATTAGAAAGG CTATTGGCAT ATGCCCCACG GAGAAAATCT ACACAGCGCT GGCTAAAGCT 
GACTCCTTCC TACTTGAAAG GACCCTGCCT TCCAAGAGCA CCTTCACCCT GGCCATTGTG 
GCCTATGCTC TCTCCCTGGG AGACAGAACC CACCCGAAGT TTCGTTCTAT TGTGTCAGCC 
CTGAAGAGGG AAGCTTTGGT TAAAGGAGAC CCGCCCATTT ACCGTTTCTG GAGAGACACT 
CTCCAACGTC CAGACAGCTC AGCACCCAAC AGCGGCACAG CAGGTATGGT AGAAACCACG 
GCCTATGCTT TGCTCACCAG CCTGAACCTG AAGGAGACGA GTTATGTCAA CCCGATCATC 
AAGTGGCTAT CTGAGGAGCA GAGGTATGGA GGCGGCTTTT ATTCCACCCA GGATACCATT 
AACGCCATCG AGGGCCTGAC AGAGTATTCA CTCCTGGTTA AACAACTTCA TTTGGATATG 
GATATCAATG TCTCCTACAA ACACAAAGGG GATTTCTACC AGTATAAAGT GACAGAGAAG 
AACTTCCTCG GGAGGCCAGT GGAGGTACCC CTCAATGATG ACCTCATCGT CACCACAGGC 
TATAGCAGTG GCTTGGCTAC AGTATATGTA AAAACTGTGG TTCACAAAAC TAGTGTCGCT 
GAGGAATTTT GCAGCTTTTA CTTGAAAATT GATACCCAAG AAGTTGAAGC CTCCAGCTAC 
CTCAGCTACA GTGACTCGGG ACACAAGCGC ATAATAGCCT GTGCCAGCTA CAAGCCCAGC 
AAGGAGGAGT CAGCATCTGG GTCCTCCCAT GCAGTAATGG ATATACTGCT GCCGACCGGA 
ATCGGAGCAA ACCAAGAAGA TTTACGAGCT CTTGTGGAAG GAGTAGATCA ACTCCTAACT 
GATTACCAGA TCAAAGACAG TCATGTTATT CTGCAATTGA ATTCGATTCC CTCCAGAGAT 
TTCCTTTGTG TTCGGTTCCG GATATTTGAA CTTTTCCAAG TTGGGTTTCT GAATCCTGCT 
ACGTTCACGG TGTACGAGTA TCACAGACCA GATAAGCAGT GTACCATGAT TTACAGCACT 
TCTGACACCA ACCTTCAGAG AGTCTGTGAA GGAGCGGCAT GCAAATGCGT TGAAGCTGAT 
TGTGGGCAAC TGCAGGCAGA ACTGGACCTG GCCATCTCTG CAGACACCAG GAAAGAAACA 
GCATGTAAAC CAGAGATTGC ATATGCTTAT AAGGTCAGGA TCACGTCGGC CACGGAAGAA 
AACATTTTTG TCAAGTACAC TGCGACGCTT CTGGATATTT ACAAAACAGG GGAAGCCGCT 
GCTGAGAAGG ACTCTGAGAT CACCTTCATT AAAAAGATAA GCTGTACCAA CGCCAACCTG 
GTGAAAGGAA AGCAATATTT AATCATGGGC AAAGAGGCTC TGCAGATCAA ACACAATTTC 
AGTTTCAAGT ATATATACCC TCTAGATTCC TCCACCTGGA TTGAATATTG GCCCACAGAC 
ACAACGTGTC CATCCTGCCA AGCGTTTGTA GCTAATTTGG ACGAGTTCGC TGAAGACATC 
TTTCTAAATG GCTGTGAAAA TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444179.1
CDS	59..5125
Translation	MPLPAMGLWGLLCLLIFLDKTWGQEQTYVISAPKIFRVGSSENVVIQAHGYTEAFDATISLKSYPDKKVTYSSGYVNLSPENKFQNSALLTLPPKQFPRDENPVSHVYLEVVSMHFSKSKKIPITYDNGFLFIHTDKPVYTPDQSVKIRVYSLSDDLKPAKRETVLTFVDPEGTEVDIVEENDYTGIISFPDFKIPSNPKYGVWTIKAKYKKDFTTTGTAYFEVKEYVLPRFSVSIEPESNFIGYKNFKNFEITVKARYFYNKMVPDAEVYIFFGLREDIKEDEKQMMHKAMQAATLVNGVAQISFDSETAVKELSYESLEDLNNKYLYIAVTVTESSGGFSEEAEIPGIKYVLSPYTLNLVATPLFLKPGIPFSIKVQVKDSLEQLVGGVPVTLMAQTVNVNQETSDLEPKRSITHSADGVASFVVNLPSEVTSLKFEVKTDAPELPEENQASKEYEAVTYSSLSQSYIYIGWTENYKPMLVGEYLNIIVTPKSPYIDKITHYNYLILSKGKIVQYGTKEKLLYSSYQNINIPVTQDMVPSARLLVYYIVTGEQTAELVADAVWINIEEKCGNQLQVHLSPDKDVYSPGQTVSLDMVTEADSWVALSAVDSAVYGVRGKAKRAMQRVFQAFDDKSDLGCGAGGGRDNVDVFHLAGLTFLTNANADDSQYHDDSCKEILRPKRDLQLLHQKVEEQAAKYKHRVPKKCCYDGARENKYETCEQRVARVTIGPHCIRAFNECCTIADKIRKESHHKGMLLGRIQIKALLPVMKAEIRSYFPESWLWEVHRVPKRNQLQVALPDSLTTWEIQGIGISDNGICVADTLKAKVFKDVFLEMNIPYSVVRGEQIQLKGTVYNYRTSGTMFCVKMSAVEGICTPGSSAASPQTSRSSRCVRQRIEGSSSHLVTFSLLPLEIGLHSINFSLETSFGKEILVKTLRVVPEGIKRESYAGVTLDPRGVYGIVNRRKEFPYRIPLDLVPKTNVKRILSVKGLLIGEFLSTVLSKEGIDILTHLPKGSAEAELMSIVPVFYVFHYLEAGNHWNIFHPDTLARKQSLQKKIKEGLVSVMSYRNADYSYSMWKGASSSAWLTAFALRVLGQVNKYVKQDQYSICNSLLWLIEKCQLENGSFKENSQYLPIKLQGTLPAEAQENTLYLTAFSVIGIRKAIGICPTEKIYTALAKADSFLLERTLPSKSTFTLAIVAYALSLGDRTHPKFRSIVSALKREALVKGDPPIYRFWRDTLQRPDSSAPNSGTAGMVETTAYALLTSLNLKETSYVNPIIKWLSEEQRYGGGFYSTQDTINAIEGLTEYSLLVKQLHLDMDINVSYKHKGDFYQYKVTEKNFLGRPVEVPLNDDLIVTTGYSSGLATVYVKTVVHKTSVAEEFCSFYLKIDTQEVEASSYLSYSDSGHKRIIACASYKPSKEESASGSSHAVMDILLPTGIGANQEDLRALVEGVDQLLTDYQIKDSHVILQLNSIPSRDFLCVRFRIFELFQVGFLNPATFTVYEYHRPDKQCTMIYSTSDTNLQRVCEGAACKCVEADCGQLQAELDLAISADTRKETACKPEIAYAYKVRITSATEENIFVKYTATLLDIYKTGEAAAEKDSEITFIKKISCTNANLVKGKQYLIMGKEALQIKHNFSFKYIYPLDSSTWIEYWPTDTTCPSCQAFVANLDEFAEDIFLNGCENA

Target ORF information:

RefSeq Version	NM_053020.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus complement C5 (C5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053020.1

ORF Insert Sequence:

ATGCCGCTTC CAGCCATGGG CCTTTGGGGA CTACTTTGCC TTTTAATTTT CCTGGACAAG 
ACCTGGGGAC AGGAACAAAC CTACGTCATT TCAGCACCCA AAATCTTCCG GGTCGGATCA 
TCCGAAAATG TCGTAATTCA AGCCCATGGC TACACCGAAG CCTTTGACGC AACGATCTCT 
CTGAAAAGCT ATCCTGACAA AAAAGTGACC TACTCTTCCG GCTATGTTAA CTTGTCGCCG 
GAAAACAAAT TCCAGAACTC AGCACTGTTG ACATTACCGC CCAAACAATT TCCCAGAGAT 
GAAAACCCAG TCTCTCACGT GTATCTGGAA GTTGTGTCAA TGCACTTTTC GAAATCGAAG 
AAAATACCAA TAACCTATGA CAATGGATTT CTCTTCATCC ATACAGACAA ACCTGTTTAC 
ACTCCAGACC AGTCAGTAAA GATTAGAGTC TACTCTCTGA GTGACGACTT GAAGCCAGCC 
AAACGGGAGA CTGTCTTAAC TTTCGTAGAT CCCGAAGGAA CAGAAGTTGA CATCGTAGAA 
GAAAATGATT ATACTGGAAT TATCTCTTTT CCTGACTTCA AGATTCCATC TAATCCCAAG 
TATGGTGTAT GGACAATTAA AGCTAAATAT AAGAAGGATT TTACAACGAC TGGAACTGCA 
TACTTTGAAG TTAAAGAATA TGTCTTGCCC CGATTCTCTG TATCAATAGA ACCAGAAAGC 
AACTTCATTG GCTATAAGAA CTTTAAGAAC TTTGAAATCA CTGTGAAAGC AAGATATTTT 
TATAATAAAA TGGTCCCCGA TGCTGAAGTC TATATCTTTT TTGGGTTGAG AGAGGACATA 
AAAGAGGATG AGAAACAGAT GATGCATAAA GCCATGCAAG CCGCAACGTT GGTCAATGGA 
GTTGCTCAGA TCTCTTTTGA CTCTGAAACA GCAGTGAAAG AGCTGTCATA TGAAAGTCTA 
GAAGATCTAA ACAATAAGTA CCTTTATATT GCTGTAACAG TCACAGAGTC TTCAGGTGGG 
TTTTCGGAAG AGGCAGAAAT TCCTGGCATC AAATACGTCC TCTCTCCCTA TACACTGAAT 
TTGGTCGCTA CCCCTCTTTT CCTGAAGCCT GGGATTCCAT TTTCCATCAA GGTACAGGTT 
AAGGATTCAC TCGAGCAGTT GGTAGGAGGG GTCCCAGTAA CTCTGATGGC ACAAACAGTC 
AATGTGAATC AAGAGACATC TGACTTGGAA CCAAAGAGGA GCATCACACA CTCTGCTGAT 
GGAGTGGCTT CATTTGTGGT GAACCTCCCA TCAGAAGTGA CATCACTGAA GTTTGAGGTC 
AAAACTGATG CCCCGGAACT TCCCGAAGAA AATCAAGCCA GCAAAGAATA TGAAGCAGTT 
ACATACTCAT CCCTCAGCCA GAGTTACATT TACATTGGCT GGACTGAAAA CTACAAGCCC 
ATGCTTGTGG GAGAATATCT GAATATTATC GTCACCCCCA AGAGTCCATA TATTGACAAA 
ATAACTCACT ATAATTACTT GATTTTATCC AAAGGCAAAA TTGTACAGTA TGGCACAAAG 
GAGAAACTTC TCTATTCATC TTATCAAAAT ATAAACATCC CAGTGACACA GGACATGGTT 
CCTTCAGCGC GGCTCCTGGT CTATTACATA GTCACGGGGG AGCAGACAGC AGAATTGGTG 
GCTGACGCAG TCTGGATAAA CATTGAGGAG AAGTGTGGCA ACCAGCTCCA GGTCCATCTG 
TCTCCAGATA AAGACGTGTA TTCTCCAGGC CAAACTGTGT CCCTTGACAT GGTGACTGAA 
GCAGACTCAT GGGTGGCACT ATCTGCGGTG GACAGCGCTG TGTATGGAGT CCGGGGAAAA 
GCCAAAAGGG CCATGCAAAG AGTGTTCCAA GCTTTTGATG ACAAGAGTGA CCTGGGCTGT 
GGGGCAGGTG GTGGCCGTGA CAATGTAGAT GTATTCCATC TAGCTGGGCT CACCTTCCTC 
ACCAATGCAA ACGCAGATGA CTCCCAATAC CACGATGACT CTTGTAAGGA AATTCTCAGG 
CCAAAGAGAG ACCTGCAGCT CCTGCATCAG AAAGTGGAAG AACAAGCTGC TAAATACAAA 
CACCGTGTGC CCAAGAAATG CTGTTATGAT GGAGCCCGAG AAAACAAATA CGAAACCTGT 
GAGCAGCGAG TTGCCCGGGT GACCATAGGC CCACACTGCA TCAGGGCCTT CAACGAGTGT 
TGTACTATTG CGGATAAGAT CCGAAAAGAA AGCCACCACA AAGGCATGCT GTTGGGAAGG 
ATCCAAATAA AGGCCCTGTT ACCAGTGATG AAGGCAGAAA TCCGAAGCTA CTTTCCAGAG 
AGCTGGCTAT GGGAAGTTCA TCGTGTTCCC AAAAGAAACC AGCTGCAGGT TGCACTGCCT 
GACTCACTGA CGACCTGGGA AATTCAAGGC ATCGGCATCT CAGACAATGG TATATGTGTT 
GCTGACACAC TCAAGGCAAA GGTGTTCAAA GATGTCTTCC TGGAGATGAA CATACCATAT 
TCTGTTGTAC GAGGGGAGCA GATCCAATTG AAGGGAACCG TTTACAATTA TAGGACCTCT 
GGGACAATGT TCTGTGTTAA AATGTCTGCC GTGGAGGGAA TCTGCACTCC AGGAAGCTCG 
GCTGCTAGCC CTCAGACCTC TAGGTCCTCC AGATGTGTGC GCCAGAGAAT AGAGGGCTCC 
TCCAGTCACT TGGTGACCTT CAGCCTGCTT CCTCTGGAAA TTGGCCTTCA CTCCATAAAC 
TTCTCACTAG AGACTTCATT TGGGAAAGAA ATCTTAGTGA AGACATTACG GGTAGTGCCA 
GAAGGGATCA AAAGGGAAAG CTATGCTGGT GTGACTCTGG ACCCCAGGGG AGTTTATGGT 
ATTGTTAACA GACGAAAGGA ATTCCCATAC AGGATACCAT TAGATTTGGT CCCCAAAACC 
AACGTCAAAA GGATTTTGAG TGTAAAAGGA CTGCTTATAG GGGAATTCTT GTCCACGGTT 
CTGAGTAAAG AAGGCATCGA CATCCTAACC CACCTCCCCA AGGGCAGCGC CGAGGCAGAA 
CTCATGAGCA TAGTCCCGGT GTTCTACGTT TTCCACTACC TGGAAGCAGG AAACCATTGG 
AATATTTTCC ACCCTGATAC GTTAGCTAGA AAACAGAGCC TGCAGAAAAA AATAAAAGAA 
GGGCTGGTGA GCGTCATGTC CTACAGAAAC GCTGACTATT CCTACAGCAT GTGGAAGGGA 
GCAAGCTCTA GTGCCTGGCT GACAGCTTTT GCTCTGAGAG TGCTTGGACA GGTGAACAAG 
TATGTGAAAC AAGACCAATA CTCGATCTGT AACTCCTTGT TATGGCTGAT TGAGAAGTGT 
CAGCTGGAAA ACGGATCTTT CAAGGAAAAT TCCCAATATC TACCAATAAA ATTACAGGGT 
ACTTTGCCTG CTGAAGCCCA AGAGAACACT TTATATCTTA CAGCCTTTTC TGTGATTGGA 
ATTAGAAAGG CTATTGGCAT ATGCCCCACG GAGAAAATCT ACACAGCGCT GGCTAAAGCT 
GACTCCTTCC TACTTGAAAG GACCCTGCCT TCCAAGAGCA CCTTCACCCT GGCCATTGTG 
GCCTATGCTC TCTCCCTGGG AGACAGAACC CACCCGAAGT TTCGTTCTAT TGTGTCAGCC 
CTGAAGAGGG AAGCTTTGGT TAAAGGAGAC CCGCCCATTT ACCGTTTCTG GAGAGACACT 
CTCCAACGTC CAGACAGCTC AGCACCCAAC AGCGGCACAG CAGGTATGGT AGAAACCACG 
GCCTATGCTT TGCTCACCAG CCTGAACCTG AAGGAGACGA GTTATGTCAA CCCGATCATC 
AAGTGGCTAT CTGAGGAGCA GAGGTATGGA GGCGGCTTTT ATTCCACCCA GGATACCATT 
AACGCCATCG AGGGCCTGAC AGAGTATTCA CTCCTGGTTA AACAACTTCA TTTGGATATG 
GATATCAATG TCTCCTACAA ACACAAAGGG GATTTCTACC AGTATAAAGT GACAGAGAAG 
AACTTCCTCG GGAGGCCAGT GGAGGTACCC CTCAATGATG ACCTCATCGT CACCACAGGC 
TATAGCAGTG GCTTGGCTAC AGTATATGTA AAAACTGTGG TTCACAAAAC TAGTGTCGCT 
GAGGAATTTT GCAGCTTTTA CTTGAAAATT GATACCCAAG AAGTTGAAGC CTCCAGCTAC 
CTCAGCTACA GTGACTCGGG ACACAAGCGC ATAATAGCCT GTGCCAGCTA CAAGCCCAGC 
AAGGAGGAGT CAGCATCTGG GTCCTCCCAT GCAGTAATGG ATATACTGCT GCCGACCGGA 
ATCGGAGCAA ACCAAGAAGA TTTACGAGCT CTTGTGGAAG GAGTAGATCA ACTCCTAACT 
GATTACCAGA TCAAAGACAG TCATGTTATT CTGCAATTGA ATTCGATTCC CTCCAGAGAT 
TTCCTTTGTG TTCGGTTCCG GATATTTGAA CTTTTCCAAG TTGGGTTTCT GAATCCTGCT 
ACGTTCACGG TGTACGAGTA TCACAGACCA GATAAGCAGT GTACCATGAT TTACAGCACT 
TCTGACACCA ACCTTCAGAG AGTCTGTGAA GGAGCGGCAT GCAAATGCGT TGAAGCTGAT 
TGTGGGCAAC TGCAGGCAGA ACTGGACCTG GCCATCTCTG CAGACACCAG GAAAGAAACA 
GCATGTAAAC CAGAGATTGC ATATGCTTAT AAGGTCAGGA TCACGTCGGC CACGGAAGAA 
AACATTTTTG TCAAGTACAC TGCGACGCTT CTGGATATTT ACAAAACAGG GGAAGCCGCT 
GCTGAGAAGG ACTCTGAGAT CACCTTCATT AAAAAGATAA GCTGTACCAA CGCCAACCTG 
GTGAAAGGAA AGCAATATTT AATCATGGGC AAAGAGGCTC TGCAGATCAA ACACAATTTC 
AGTTTCAAGT ATATATACCC TCTAGATTCC TCCACCTGGA TTGAATATTG GCCCACAGAC 
ACAACGTGTC CATCCTGCCA AGCGTTTGTA GCTAATTTGG ACGAGTTCGC TGAAGACATC 
TTTCTAAATG GCTGTGAAAA TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C5 ( XM_001079130.6 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001079130.2 Rattus norvegicus complement component 5 (C5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa15554
Clone ID Related Accession (Same CDS sequence)	NM_053020.1 , XM_001079130.6
Accession Version	XM_001079130.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	complement C5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000071.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001079130.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCTTC CAGCCATGGG CCTTTGGGGA CTACTTTGCC TTTTAATTTT CCTGGACAAG 
ACCTGGGGAC AGGAACAAAC CTACGTCATT TCAGCACCCA AAATCTTCCG GGTCGGATCA 
TCCGAAAATG TCGTAATTCA AGCCCATGGC TACACCGAAG CCTTTGACGC AACGATCTCT 
CTGAAAAGCT ATCCTGACAA AAAAGTGACC TACTCTTCCG GCTATGTTAA CTTGTCGCCG 
GAAAACAAAT TCCAGAACTC AGCACTGTTG ACATTACCGC CCAAACAATT TCCCAGAGAT 
GAAAACCCAG TCTCTCACGT GTATCTGGAA GTTGTGTCAA TGCACTTTTC GAAATCGAAG 
AAAATACCAA TAACCTATGA CAATGGATTT CTCTTCATCC ATACAGACAA ACCTGTTTAC 
ACTCCAGACC AGTCAGTAAA GATTAGAGTC TACTCTCTGA GTGACGACTT GAAGCCAGCC 
AAACGGGAGA CTGTCTTAAC TTTCGTAGAT CCCGAAGGAA CAGAAGTTGA CATCGTAGAA 
GAAAATGATT ATACTGGAAT TATCTCTTTT CCTGACTTCA AGATTCCATC TAATCCCAAG 
TATGGTGTAT GGACAATTAA AGCTAAATAT AAGAAGGATT TTACAACGAC TGGAACTGCA 
TACTTTGAAG TTAAAGAATA TGTCTTGCCC CGATTCTCTG TATCAATAGA ACCAGAAAGC 
AACTTCATTG GCTATAAGAA CTTTAAGAAC TTTGAAATCA CTGTGAAAGC AAGATATTTT 
TATAATAAAA TGGTCCCCGA TGCTGAAGTC TATATCTTTT TTGGGTTGAG AGAGGACATA 
AAAGAGGATG AGAAACAGAT GATGCATAAA GCCATGCAAG CCGCAACGTT GGTCAATGGA 
GTTGCTCAGA TCTCTTTTGA CTCTGAAACA GCAGTGAAAG AGCTGTCATA TGAAAGTCTA 
GAAGATCTAA ACAATAAGTA CCTTTATATT GCTGTAACAG TCACAGAGTC TTCAGGTGGG 
TTTTCGGAAG AGGCAGAAAT TCCTGGCATC AAATACGTCC TCTCTCCCTA TACACTGAAT 
TTGGTCGCTA CCCCTCTTTT CCTGAAGCCT GGGATTCCAT TTTCCATCAA GGTACAGGTT 
AAGGATTCAC TCGAGCAGTT GGTAGGAGGG GTCCCAGTAA CTCTGATGGC ACAAACAGTC 
AATGTGAATC AAGAGACATC TGACTTGGAA CCAAAGAGGA GCATCACACA CTCTGCTGAT 
GGAGTGGCTT CATTTGTGGT GAACCTCCCA TCAGAAGTGA CATCACTGAA GTTTGAGGTC 
AAAACTGATG CCCCGGAACT TCCCGAAGAA AATCAAGCCA GCAAAGAATA TGAAGCAGTT 
ACATACTCAT CCCTCAGCCA GAGTTACATT TACATTGGCT GGACTGAAAA CTACAAGCCC 
ATGCTTGTGG GAGAATATCT GAATATTATC GTCACCCCCA AGAGTCCATA TATTGACAAA 
ATAACTCACT ATAATTACTT GATTTTATCC AAAGGCAAAA TTGTACAGTA TGGCACAAAG 
GAGAAACTTC TCTATTCATC TTATCAAAAT ATAAACATCC CAGTGACACA GGACATGGTT 
CCTTCAGCGC GGCTCCTGGT CTATTACATA GTCACGGGGG AGCAGACAGC AGAATTGGTG 
GCTGACGCAG TCTGGATAAA CATTGAGGAG AAGTGTGGCA ACCAGCTCCA GGTCCATCTG 
TCTCCAGATA AAGACGTGTA TTCTCCAGGC CAAACTGTGT CCCTTGACAT GGTGACTGAA 
GCAGACTCAT GGGTGGCACT ATCTGCGGTG GACAGCGCTG TGTATGGAGT CCGGGGAAAA 
GCCAAAAGGG CCATGCAAAG AGTGTTCCAA GCTTTTGATG ACAAGAGTGA CCTGGGCTGT 
GGGGCAGGTG GTGGCCGTGA CAATGTAGAT GTATTCCATC TAGCTGGGCT CACCTTCCTC 
ACCAATGCAA ACGCAGATGA CTCCCAATAC CACGATGACT CTTGTAAGGA AATTCTCAGG 
CCAAAGAGAG ACCTGCAGCT CCTGCATCAG AAAGTGGAAG AACAAGCTGC TAAATACAAA 
CACCGTGTGC CCAAGAAATG CTGTTATGAT GGAGCCCGAG AAAACAAATA CGAAACCTGT 
GAGCAGCGAG TTGCCCGGGT GACCATAGGC CCACACTGCA TCAGGGCCTT CAACGAGTGT 
TGTACTATTG CGGATAAGAT CCGAAAAGAA AGCCACCACA AAGGCATGCT GTTGGGAAGG 
ATCCAAATAA AGGCCCTGTT ACCAGTGATG AAGGCAGAAA TCCGAAGCTA CTTTCCAGAG 
AGCTGGCTAT GGGAAGTTCA TCGTGTTCCC AAAAGAAACC AGCTGCAGGT TGCACTGCCT 
GACTCACTGA CGACCTGGGA AATTCAAGGC ATCGGCATCT CAGACAATGG TATATGTGTT 
GCTGACACAC TCAAGGCAAA GGTGTTCAAA GATGTCTTCC TGGAGATGAA CATACCATAT 
TCTGTTGTAC GAGGGGAGCA GATCCAATTG AAGGGAACCG TTTACAATTA TAGGACCTCT 
GGGACAATGT TCTGTGTTAA AATGTCTGCC GTGGAGGGAA TCTGCACTCC AGGAAGCTCG 
GCTGCTAGCC CTCAGACCTC TAGGTCCTCC AGATGTGTGC GCCAGAGAAT AGAGGGCTCC 
TCCAGTCACT TGGTGACCTT CAGCCTGCTT CCTCTGGAAA TTGGCCTTCA CTCCATAAAC 
TTCTCACTAG AGACTTCATT TGGGAAAGAA ATCTTAGTGA AGACATTACG GGTAGTGCCA 
GAAGGGATCA AAAGGGAAAG CTATGCTGGT GTGACTCTGG ACCCCAGGGG AGTTTATGGT 
ATTGTTAACA GACGAAAGGA ATTCCCATAC AGGATACCAT TAGATTTGGT CCCCAAAACC 
AACGTCAAAA GGATTTTGAG TGTAAAAGGA CTGCTTATAG GGGAATTCTT GTCCACGGTT 
CTGAGTAAAG AAGGCATCGA CATCCTAACC CACCTCCCCA AGGGCAGCGC CGAGGCAGAA 
CTCATGAGCA TAGTCCCGGT GTTCTACGTT TTCCACTACC TGGAAGCAGG AAACCATTGG 
AATATTTTCC ACCCTGATAC GTTAGCTAGA AAACAGAGCC TGCAGAAAAA AATAAAAGAA 
GGGCTGGTGA GCGTCATGTC CTACAGAAAC GCTGACTATT CCTACAGCAT GTGGAAGGGA 
GCAAGCTCTA GTGCCTGGCT GACAGCTTTT GCTCTGAGAG TGCTTGGACA GGTGAACAAG 
TATGTGAAAC AAGACCAATA CTCGATCTGT AACTCCTTGT TATGGCTGAT TGAGAAGTGT 
CAGCTGGAAA ACGGATCTTT CAAGGAAAAT TCCCAATATC TACCAATAAA ATTACAGGGT 
ACTTTGCCTG CTGAAGCCCA AGAGAACACT TTATATCTTA CAGCCTTTTC TGTGATTGGA 
ATTAGAAAGG CTATTGGCAT ATGCCCCACG GAGAAAATCT ACACAGCGCT GGCTAAAGCT 
GACTCCTTCC TACTTGAAAG GACCCTGCCT TCCAAGAGCA CCTTCACCCT GGCCATTGTG 
GCCTATGCTC TCTCCCTGGG AGACAGAACC CACCCGAAGT TTCGTTCTAT TGTGTCAGCC 
CTGAAGAGGG AAGCTTTGGT TAAAGGAGAC CCGCCCATTT ACCGTTTCTG GAGAGACACT 
CTCCAACGTC CAGACAGCTC AGCACCCAAC AGCGGCACAG CAGGTATGGT AGAAACCACG 
GCCTATGCTT TGCTCACCAG CCTGAACCTG AAGGAGACGA GTTATGTCAA CCCGATCATC 
AAGTGGCTAT CTGAGGAGCA GAGGTATGGA GGCGGCTTTT ATTCCACCCA GGATACCATT 
AACGCCATCG AGGGCCTGAC AGAGTATTCA CTCCTGGTTA AACAACTTCA TTTGGATATG 
GATATCAATG TCTCCTACAA ACACAAAGGG GATTTCTACC AGTATAAAGT GACAGAGAAG 
AACTTCCTCG GGAGGCCAGT GGAGGTACCC CTCAATGATG ACCTCATCGT CACCACAGGC 
TATAGCAGTG GCTTGGCTAC AGTATATGTA AAAACTGTGG TTCACAAAAC TAGTGTCGCT 
GAGGAATTTT GCAGCTTTTA CTTGAAAATT GATACCCAAG AAGTTGAAGC CTCCAGCTAC 
CTCAGCTACA GTGACTCGGG ACACAAGCGC ATAATAGCCT GTGCCAGCTA CAAGCCCAGC 
AAGGAGGAGT CAGCATCTGG GTCCTCCCAT GCAGTAATGG ATATACTGCT GCCGACCGGA 
ATCGGAGCAA ACCAAGAAGA TTTACGAGCT CTTGTGGAAG GAGTAGATCA ACTCCTAACT 
GATTACCAGA TCAAAGACAG TCATGTTATT CTGCAATTGA ATTCGATTCC CTCCAGAGAT 
TTCCTTTGTG TTCGGTTCCG GATATTTGAA CTTTTCCAAG TTGGGTTTCT GAATCCTGCT 
ACGTTCACGG TGTACGAGTA TCACAGACCA GATAAGCAGT GTACCATGAT TTACAGCACT 
TCTGACACCA ACCTTCAGAG AGTCTGTGAA GGAGCGGCAT GCAAATGCGT TGAAGCTGAT 
TGTGGGCAAC TGCAGGCAGA ACTGGACCTG GCCATCTCTG CAGACACCAG GAAAGAAACA 
GCATGTAAAC CAGAGATTGC ATATGCTTAT AAGGTCAGGA TCACGTCGGC CACGGAAGAA 
AACATTTTTG TCAAGTACAC TGCGACGCTT CTGGATATTT ACAAAACAGG GGAAGCCGCT 
GCTGAGAAGG ACTCTGAGAT CACCTTCATT AAAAAGATAA GCTGTACCAA CGCCAACCTG 
GTGAAAGGAA AGCAATATTT AATCATGGGC AAAGAGGCTC TGCAGATCAA ACACAATTTC 
AGTTTCAAGT ATATATACCC TCTAGATTCC TCCACCTGGA TTGAATATTG GCCCACAGAC 
ACAACGTGTC CATCCTGCCA AGCGTTTGTA GCTAATTTGG ACGAGTTCGC TGAAGACATC 
TTTCTAAATG GCTGTGAAAA TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001079130.2
CDS	59..5125
Translation	MPLPAMGLWGLLCLLIFLDKTWGQEQTYVISAPKIFRVGSSENVVIQAHGYTEAFDATISLKSYPDKKVTYSSGYVNLSPENKFQNSALLTLPPKQFPRDENPVSHVYLEVVSMHFSKSKKIPITYDNGFLFIHTDKPVYTPDQSVKIRVYSLSDDLKPAKRETVLTFVDPEGTEVDIVEENDYTGIISFPDFKIPSNPKYGVWTIKAKYKKDFTTTGTAYFEVKEYVLPRFSVSIEPESNFIGYKNFKNFEITVKARYFYNKMVPDAEVYIFFGLREDIKEDEKQMMHKAMQAATLVNGVAQISFDSETAVKELSYESLEDLNNKYLYIAVTVTESSGGFSEEAEIPGIKYVLSPYTLNLVATPLFLKPGIPFSIKVQVKDSLEQLVGGVPVTLMAQTVNVNQETSDLEPKRSITHSADGVASFVVNLPSEVTSLKFEVKTDAPELPEENQASKEYEAVTYSSLSQSYIYIGWTENYKPMLVGEYLNIIVTPKSPYIDKITHYNYLILSKGKIVQYGTKEKLLYSSYQNINIPVTQDMVPSARLLVYYIVTGEQTAELVADAVWINIEEKCGNQLQVHLSPDKDVYSPGQTVSLDMVTEADSWVALSAVDSAVYGVRGKAKRAMQRVFQAFDDKSDLGCGAGGGRDNVDVFHLAGLTFLTNANADDSQYHDDSCKEILRPKRDLQLLHQKVEEQAAKYKHRVPKKCCYDGARENKYETCEQRVARVTIGPHCIRAFNECCTIADKIRKESHHKGMLLGRIQIKALLPVMKAEIRSYFPESWLWEVHRVPKRNQLQVALPDSLTTWEIQGIGISDNGICVADTLKAKVFKDVFLEMNIPYSVVRGEQIQLKGTVYNYRTSGTMFCVKMSAVEGICTPGSSAASPQTSRSSRCVRQRIEGSSSHLVTFSLLPLEIGLHSINFSLETSFGKEILVKTLRVVPEGIKRESYAGVTLDPRGVYGIVNRRKEFPYRIPLDLVPKTNVKRILSVKGLLIGEFLSTVLSKEGIDILTHLPKGSAEAELMSIVPVFYVFHYLEAGNHWNIFHPDTLARKQSLQKKIKEGLVSVMSYRNADYSYSMWKGASSSAWLTAFALRVLGQVNKYVKQDQYSICNSLLWLIEKCQLENGSFKENSQYLPIKLQGTLPAEAQENTLYLTAFSVIGIRKAIGICPTEKIYTALAKADSFLLERTLPSKSTFTLAIVAYALSLGDRTHPKFRSIVSALKREALVKGDPPIYRFWRDTLQRPDSSAPNSGTAGMVETTAYALLTSLNLKETSYVNPIIKWLSEEQRYGGGFYSTQDTINAIEGLTEYSLLVKQLHLDMDINVSYKHKGDFYQYKVTEKNFLGRPVEVPLNDDLIVTTGYSSGLATVYVKTVVHKTSVAEEFCSFYLKIDTQEVEASSYLSYSDSGHKRIIACASYKPSKEESASGSSHAVMDILLPTGIGANQEDLRALVEGVDQLLTDYQIKDSHVILQLNSIPSRDFLCVRFRIFELFQVGFLNPATFTVYEYHRPDKQCTMIYSTSDTNLQRVCEGAACKCVEADCGQLQAELDLAISADTRKETACKPEIAYAYKVRITSATEENIFVKYTATLLDIYKTGEAAAEKDSEITFIKKISCTNANLVKGKQYLIMGKEALQIKHNFSFKYIYPLDSSTWIEYWPTDTTCPSCQAFVANLDEFAEDIFLNGCENA

Target ORF information:

RefSeq Version	XM_001079130.6
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus complement component 5 (C5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001079130.6

ORF Insert Sequence:

ATGCCGCTTC CAGCCATGGG CCTTTGGGGA CTACTTTGCC TTTTAATTTT CCTGGACAAG 
ACCTGGGGAC AGGAACAAAC CTACGTCATT TCAGCACCCA AAATCTTCCG GGTCGGATCA 
TCCGAAAATG TCGTAATTCA AGCCCATGGC TACACCGAAG CCTTTGACGC AACGATCTCT 
CTGAAAAGCT ATCCTGACAA AAAAGTGACC TACTCTTCCG GCTATGTTAA CTTGTCGCCG 
GAAAACAAAT TCCAGAACTC AGCACTGTTG ACATTACCGC CCAAACAATT TCCCAGAGAT 
GAAAACCCAG TCTCTCACGT GTATCTGGAA GTTGTGTCAA TGCACTTTTC GAAATCGAAG 
AAAATACCAA TAACCTATGA CAATGGATTT CTCTTCATCC ATACAGACAA ACCTGTTTAC 
ACTCCAGACC AGTCAGTAAA GATTAGAGTC TACTCTCTGA GTGACGACTT GAAGCCAGCC 
AAACGGGAGA CTGTCTTAAC TTTCGTAGAT CCCGAAGGAA CAGAAGTTGA CATCGTAGAA 
GAAAATGATT ATACTGGAAT TATCTCTTTT CCTGACTTCA AGATTCCATC TAATCCCAAG 
TATGGTGTAT GGACAATTAA AGCTAAATAT AAGAAGGATT TTACAACGAC TGGAACTGCA 
TACTTTGAAG TTAAAGAATA TGTCTTGCCC CGATTCTCTG TATCAATAGA ACCAGAAAGC 
AACTTCATTG GCTATAAGAA CTTTAAGAAC TTTGAAATCA CTGTGAAAGC AAGATATTTT 
TATAATAAAA TGGTCCCCGA TGCTGAAGTC TATATCTTTT TTGGGTTGAG AGAGGACATA 
AAAGAGGATG AGAAACAGAT GATGCATAAA GCCATGCAAG CCGCAACGTT GGTCAATGGA 
GTTGCTCAGA TCTCTTTTGA CTCTGAAACA GCAGTGAAAG AGCTGTCATA TGAAAGTCTA 
GAAGATCTAA ACAATAAGTA CCTTTATATT GCTGTAACAG TCACAGAGTC TTCAGGTGGG 
TTTTCGGAAG AGGCAGAAAT TCCTGGCATC AAATACGTCC TCTCTCCCTA TACACTGAAT 
TTGGTCGCTA CCCCTCTTTT CCTGAAGCCT GGGATTCCAT TTTCCATCAA GGTACAGGTT 
AAGGATTCAC TCGAGCAGTT GGTAGGAGGG GTCCCAGTAA CTCTGATGGC ACAAACAGTC 
AATGTGAATC AAGAGACATC TGACTTGGAA CCAAAGAGGA GCATCACACA CTCTGCTGAT 
GGAGTGGCTT CATTTGTGGT GAACCTCCCA TCAGAAGTGA CATCACTGAA GTTTGAGGTC 
AAAACTGATG CCCCGGAACT TCCCGAAGAA AATCAAGCCA GCAAAGAATA TGAAGCAGTT 
ACATACTCAT CCCTCAGCCA GAGTTACATT TACATTGGCT GGACTGAAAA CTACAAGCCC 
ATGCTTGTGG GAGAATATCT GAATATTATC GTCACCCCCA AGAGTCCATA TATTGACAAA 
ATAACTCACT ATAATTACTT GATTTTATCC AAAGGCAAAA TTGTACAGTA TGGCACAAAG 
GAGAAACTTC TCTATTCATC TTATCAAAAT ATAAACATCC CAGTGACACA GGACATGGTT 
CCTTCAGCGC GGCTCCTGGT CTATTACATA GTCACGGGGG AGCAGACAGC AGAATTGGTG 
GCTGACGCAG TCTGGATAAA CATTGAGGAG AAGTGTGGCA ACCAGCTCCA GGTCCATCTG 
TCTCCAGATA AAGACGTGTA TTCTCCAGGC CAAACTGTGT CCCTTGACAT GGTGACTGAA 
GCAGACTCAT GGGTGGCACT ATCTGCGGTG GACAGCGCTG TGTATGGAGT CCGGGGAAAA 
GCCAAAAGGG CCATGCAAAG AGTGTTCCAA GCTTTTGATG ACAAGAGTGA CCTGGGCTGT 
GGGGCAGGTG GTGGCCGTGA CAATGTAGAT GTATTCCATC TAGCTGGGCT CACCTTCCTC 
ACCAATGCAA ACGCAGATGA CTCCCAATAC CACGATGACT CTTGTAAGGA AATTCTCAGG 
CCAAAGAGAG ACCTGCAGCT CCTGCATCAG AAAGTGGAAG AACAAGCTGC TAAATACAAA 
CACCGTGTGC CCAAGAAATG CTGTTATGAT GGAGCCCGAG AAAACAAATA CGAAACCTGT 
GAGCAGCGAG TTGCCCGGGT GACCATAGGC CCACACTGCA TCAGGGCCTT CAACGAGTGT 
TGTACTATTG CGGATAAGAT CCGAAAAGAA AGCCACCACA AAGGCATGCT GTTGGGAAGG 
ATCCAAATAA AGGCCCTGTT ACCAGTGATG AAGGCAGAAA TCCGAAGCTA CTTTCCAGAG 
AGCTGGCTAT GGGAAGTTCA TCGTGTTCCC AAAAGAAACC AGCTGCAGGT TGCACTGCCT 
GACTCACTGA CGACCTGGGA AATTCAAGGC ATCGGCATCT CAGACAATGG TATATGTGTT 
GCTGACACAC TCAAGGCAAA GGTGTTCAAA GATGTCTTCC TGGAGATGAA CATACCATAT 
TCTGTTGTAC GAGGGGAGCA GATCCAATTG AAGGGAACCG TTTACAATTA TAGGACCTCT 
GGGACAATGT TCTGTGTTAA AATGTCTGCC GTGGAGGGAA TCTGCACTCC AGGAAGCTCG 
GCTGCTAGCC CTCAGACCTC TAGGTCCTCC AGATGTGTGC GCCAGAGAAT AGAGGGCTCC 
TCCAGTCACT TGGTGACCTT CAGCCTGCTT CCTCTGGAAA TTGGCCTTCA CTCCATAAAC 
TTCTCACTAG AGACTTCATT TGGGAAAGAA ATCTTAGTGA AGACATTACG GGTAGTGCCA 
GAAGGGATCA AAAGGGAAAG CTATGCTGGT GTGACTCTGG ACCCCAGGGG AGTTTATGGT 
ATTGTTAACA GACGAAAGGA ATTCCCATAC AGGATACCAT TAGATTTGGT CCCCAAAACC 
AACGTCAAAA GGATTTTGAG TGTAAAAGGA CTGCTTATAG GGGAATTCTT GTCCACGGTT 
CTGAGTAAAG AAGGCATCGA CATCCTAACC CACCTCCCCA AGGGCAGCGC CGAGGCAGAA 
CTCATGAGCA TAGTCCCGGT GTTCTACGTT TTCCACTACC TGGAAGCAGG AAACCATTGG 
AATATTTTCC ACCCTGATAC GTTAGCTAGA AAACAGAGCC TGCAGAAAAA AATAAAAGAA 
GGGCTGGTGA GCGTCATGTC CTACAGAAAC GCTGACTATT CCTACAGCAT GTGGAAGGGA 
GCAAGCTCTA GTGCCTGGCT GACAGCTTTT GCTCTGAGAG TGCTTGGACA GGTGAACAAG 
TATGTGAAAC AAGACCAATA CTCGATCTGT AACTCCTTGT TATGGCTGAT TGAGAAGTGT 
CAGCTGGAAA ACGGATCTTT CAAGGAAAAT TCCCAATATC TACCAATAAA ATTACAGGGT 
ACTTTGCCTG CTGAAGCCCA AGAGAACACT TTATATCTTA CAGCCTTTTC TGTGATTGGA 
ATTAGAAAGG CTATTGGCAT ATGCCCCACG GAGAAAATCT ACACAGCGCT GGCTAAAGCT 
GACTCCTTCC TACTTGAAAG GACCCTGCCT TCCAAGAGCA CCTTCACCCT GGCCATTGTG 
GCCTATGCTC TCTCCCTGGG AGACAGAACC CACCCGAAGT TTCGTTCTAT TGTGTCAGCC 
CTGAAGAGGG AAGCTTTGGT TAAAGGAGAC CCGCCCATTT ACCGTTTCTG GAGAGACACT 
CTCCAACGTC CAGACAGCTC AGCACCCAAC AGCGGCACAG CAGGTATGGT AGAAACCACG 
GCCTATGCTT TGCTCACCAG CCTGAACCTG AAGGAGACGA GTTATGTCAA CCCGATCATC 
AAGTGGCTAT CTGAGGAGCA GAGGTATGGA GGCGGCTTTT ATTCCACCCA GGATACCATT 
AACGCCATCG AGGGCCTGAC AGAGTATTCA CTCCTGGTTA AACAACTTCA TTTGGATATG 
GATATCAATG TCTCCTACAA ACACAAAGGG GATTTCTACC AGTATAAAGT GACAGAGAAG 
AACTTCCTCG GGAGGCCAGT GGAGGTACCC CTCAATGATG ACCTCATCGT CACCACAGGC 
TATAGCAGTG GCTTGGCTAC AGTATATGTA AAAACTGTGG TTCACAAAAC TAGTGTCGCT 
GAGGAATTTT GCAGCTTTTA CTTGAAAATT GATACCCAAG AAGTTGAAGC CTCCAGCTAC 
CTCAGCTACA GTGACTCGGG ACACAAGCGC ATAATAGCCT GTGCCAGCTA CAAGCCCAGC 
AAGGAGGAGT CAGCATCTGG GTCCTCCCAT GCAGTAATGG ATATACTGCT GCCGACCGGA 
ATCGGAGCAA ACCAAGAAGA TTTACGAGCT CTTGTGGAAG GAGTAGATCA ACTCCTAACT 
GATTACCAGA TCAAAGACAG TCATGTTATT CTGCAATTGA ATTCGATTCC CTCCAGAGAT 
TTCCTTTGTG TTCGGTTCCG GATATTTGAA CTTTTCCAAG TTGGGTTTCT GAATCCTGCT 
ACGTTCACGG TGTACGAGTA TCACAGACCA GATAAGCAGT GTACCATGAT TTACAGCACT 
TCTGACACCA ACCTTCAGAG AGTCTGTGAA GGAGCGGCAT GCAAATGCGT TGAAGCTGAT 
TGTGGGCAAC TGCAGGCAGA ACTGGACCTG GCCATCTCTG CAGACACCAG GAAAGAAACA 
GCATGTAAAC CAGAGATTGC ATATGCTTAT AAGGTCAGGA TCACGTCGGC CACGGAAGAA 
AACATTTTTG TCAAGTACAC TGCGACGCTT CTGGATATTT ACAAAACAGG GGAAGCCGCT 
GCTGAGAAGG ACTCTGAGAT CACCTTCATT AAAAAGATAA GCTGTACCAA CGCCAACCTG 
GTGAAAGGAA AGCAATATTT AATCATGGGC AAAGAGGCTC TGCAGATCAA ACACAATTTC 
AGTTTCAAGT ATATATACCC TCTAGATTCC TCCACCTGGA TTGAATATTG GCCCACAGAC 
ACAACGTGTC CATCCTGCCA AGCGTTTGTA GCTAATTTGG ACGAGTTCGC TGAAGACATC 
TTTCTAAATG GCTGTGAAAA TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of Complement C5 in Experimental Blunt Chest Trauma-Induced Septic Acute Lung Injury (ALI).
PloS one11(7)e0159417(2016)
Kalbitz M,Karbach M,Braumueller S,Kellermann P,Gebhard F,Huber-Lang M,Perl M

Renal C3 complement component: feed forward to diabetic kidney disease.
American journal of nephrology41(1)48-56(2015)
Kelly KJ,Liu Y,Zhang J,Dominguez JH

Complement C5-deficient mice are protected from seizures in experimental cerebral malaria.
Epilepsia55(12)e139-42(2014 Dec)
Buckingham SC,Ramos TN,Barnum SR

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C5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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