Lnx1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lnx1
Entrez Gene ID	360926
Full Name	ligand of numb-protein X 1
General protein information	Preferred Names ligand of numb-protein X 1 Names E3 ubiquitin-protein ligase LNX ligand of numb-protein X 1, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p11

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599287.1	XP_017454776.1	E3 ubiquitin-protein ligase LNX isoform X1
NM_001108358.1	NP_001101828.1	E3 ubiquitin-protein ligase LNX
XM_006250903.3	XP_006250965.1	E3 ubiquitin-protein ligase LNX isoform X3
XM_006250904.3	XP_006250966.1	E3 ubiquitin-protein ligase LNX isoform X4
XM_006250902.3	XP_006250964.1	E3 ubiquitin-protein ligase LNX isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Lnx1	NP_001101828.1
Danio rerio (zebrafish)	lnx1	NP_001068581.1
Xenopus tropicalis (tropical clawed frog)	lnx1	XP_002934714.2
Pan troglodytes (chimpanzee)	LNX1	XP_001147752.1
	LNX1	XP_001091044.1
Gallus gallus (chicken)	LNX1	XP_001232234.1
Homo sapiens (human)	LNX1	NP_001119800.1
Canis lupus familiaris (dog)	LNX1	XP_849707.1
Bos taurus (cattle)	LNX1	NP_001069702.2
Mus musculus (house mouse)	Lnx1	NP_001153049.1

Gene Ontology
Bibliography

Related articles in PubMed

LNX functions as a RING type E3 ubiquitin ligase that targets the cell fate determinant Numb for ubiquitin-dependent degradation.
Nie J, McGill MA, Dermer M, Dho SE, Wolting CD, McGlade CJ
The EMBO journal21(1-2)93-102(2002 Jan)

The Lnx family proteins function as molecular scaffolds for Numb family proteins.
Rice DS, Northcutt GM, Kurschner C
Molecular and cellular neurosciences18(5)525-40(2001 Nov)

The mammalian numb phosphotyrosine-binding domain. Characterization of binding specificity and identification of a novel PDZ domain-containing numb binding protein, LNX.
Dho SE, Jacob S, Wolting CD, French MB, Rohrschneider LR, McGlade CJ
The Journal of biological chemistry273(15)9179-87(1998 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lnx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lnx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa36025	XM_006250903.3 Latest version!	Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa36026	XM_006250904.3 Latest version!	Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa07193	NM_001108358.1 Latest version!	Rattus norvegicus ligand of numb-protein X 1 (Lnx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ORa36024	XM_006250902.3 Latest version!	Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa58358	XM_017599287.1 Latest version!	Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lnx1 ( XM_006250903.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250965.1 Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa36025
Clone ID Related Accession (Same CDS sequence)	XM_006250903.3
Accession Version	XM_006250903.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase LNX isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250903.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGC TGCTGCTGCT GGTCCTGCCC TGGCTGAGTC CAGCTAACTA CATTGACAAT 
GTGGGCAACC TGCACTTCCT GTACTCGGAA CTCTGCAAAG GGGCCTCCCA CTATGGCCTG 
ACCAAAGACA GGAAAAGACG CTCACAAGAT GGCTGCCCTG ATGGTTGCGC CAGCCTCACG 
GCAACGACGC TCTCCCCAGA GGTTTCTGCT GCTGCCACCA TCTCTTTAAT GACAGACGAG 
CCTGGCCTAG ACAATCCTGC CTACACGTCC TCAGTGGAGG ATGGTGAACC GGTAACCAAC 
CCCTCAGACA CTGGCCGGAG CAACCGAACC AGGGCACGGC CCTTTGAGCG CTCCACTATG 
AGAAGCCGCT CTTTCAAAAA GATCAACCGA GCCCTGAGTG CTCTCCGAAG GACGAAGAGT 
GGAAGTGCTG TTGCCAACCA CGCTGACCAG GGCAGGGACA ACTCTGAGAA CGACACTGTC 
CCCGAAGTCT TCCCAAGGTT GTTTCACCTG ATTCCAGATG GTGAAATTAC AAGCATCAAG 
ATCAATAGAG TGGATCCCAA TGAAAGCCTC TCCATAAGGC TTGTGGGAGG CAGCGAGACC 
CCGCTGGTCC ACATCATCAT CCAGCATATT TATCGTGATG GAGTGATTGC CAGAGATGGA 
CGACTGCTGC CAGGAGACAT CATTCTCAAG GTCAATGGCA TGGACATCAG CAATGTCCCT 
CATAACTATG CCGTGCGGCT CCTGCGGCAG CCCTGCCAGG TGCTACGGCT AACGGTGCTG 
CGGGAACAGA AGTTCCGTAG CAGAAGCAAT GCTCCTGCGC CGGACTCCTA CGGACCTCGG 
GATGACAGCT TCCATGTAAT CCTCAACAAA AGCAGCCCTG AGGAGCAGCT TGGCATAAAG 
CTGGTGCGCA GGGTGGATGA GCCCGGGGTG TTCATCTTCA ACGTGCTGAA TGGCGGTGTT 
GCCGACCGAC ACGGCCAGCT GGAGGAGAAT GATCGTGTGT TGGCCATCAA CGGACATGAC 
CTTCGATTTG GCAGTCCAGA GAGCGCAGCT CATCTGATTC AGGCCAGTGA AAGACGTGTT 
CACCTCGTCG TGTCCCGCCA GGTTCGACAG CCCAGCCCAG ACATCTTTCA AGAAGCTGGC 
TGCATCAGCA ACGGCCAGCA GTCTCCTGTC TCAGGGGAGA GGAGCACATC TTCTAAGCCT 
GCAGCCACTT GTCATGAGAA GGTTGTAAGT GTCCAAAAAG ACCCCAACGA ATCTCTCGGC 
ATGACCGTTG GAGGGGGAGC ATCACACAGA GAATGGGACT TGCCCATCTA TGTCATCAGT 
GTTGAGCCCG GAGGAGTCAT AAGCAGAGAC GGAAGAATAA AAACAGGTGA CATTTTATTG 
AATGTGAATG GGATTGAGCT CACAGAGGTC AGCCGGACAG AGGCCGTTGC CATACTGAAG 
AGCACACCCT CCTCAGTGGT ACTCAAAGCC TTGGAAGTCA AGGAACAGGA GACCCAGGAA 
GACTGCAGCC CAGCAGCCCT GGACTCCAAC CACAACGTGA CCCCGCCTGG TGACTGGTCC 
CCATCCTGGG TCATGTGGCT GGAATTACCA CAGTACTTAT ATAACTGTAA GGATGTGATA 
CTACGAAGGA ACACAGCGGG AAGCCTGGGC TTCTGCATTG TGGGAGGTTA TGAAGAATAC 
AGTGGTAACC AACCTTTTTT TATCAAATCC ATCGTCGAGG GAACACCCGC GTATAATGAT 
GGAAGAATTA GATGTGGTGA CATTCTTCTT GCTGTCAACG GCAGAAGCAC GTCGGGGATG 
ATGCACGCCT GCTTAGCGAG GATGCTCAAA GAACTCAAAG GGAAAATTAC TCTCACCATT 
GCTTCCTGGC CTGGTACTTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250965.1
CDS	2524..4410
Translation	MKALLLLVLPWLSPANYIDNVGNLHFLYSELCKGASHYGLTKDRKRRSQDGCPDGCASLTATTLSPEVSAAATISLMTDEPGLDNPAYTSSVEDGEPVTNPSDTGRSNRTRARPFERSTMRSRSFKKINRALSALRRTKSGSAVANHADQGRDNSENDTVPEVFPRLFHLIPDGEITSIKINRVDPNESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQVLRLTVLREQKFRSRSNAPAPDSYGPRDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQPSPDIFQEAGCISNGQQSPVSGERSTSSKPAATCHEKVVSVQKDPNESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSTPSSVVLKALEVKEQETQEDCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLYNCKDVILRRNTAGSLGFCIVGGYEEYSGNQPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMMHACLARMLKELKGKITLTIASWPGTFL

Target ORF information:

RefSeq Version	XM_006250903.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250903.3

ORF Insert Sequence:

ATGAAGGCGC TGCTGCTGCT GGTCCTGCCC TGGCTGAGTC CAGCTAACTA CATTGACAAT 
GTGGGCAACC TGCACTTCCT GTACTCGGAA CTCTGCAAAG GGGCCTCCCA CTATGGCCTG 
ACCAAAGACA GGAAAAGACG CTCACAAGAT GGCTGCCCTG ATGGTTGCGC CAGCCTCACG 
GCAACGACGC TCTCCCCAGA GGTTTCTGCT GCTGCCACCA TCTCTTTAAT GACAGACGAG 
CCTGGCCTAG ACAATCCTGC CTACACGTCC TCAGTGGAGG ATGGTGAACC GGTAACCAAC 
CCCTCAGACA CTGGCCGGAG CAACCGAACC AGGGCACGGC CCTTTGAGCG CTCCACTATG 
AGAAGCCGCT CTTTCAAAAA GATCAACCGA GCCCTGAGTG CTCTCCGAAG GACGAAGAGT 
GGAAGTGCTG TTGCCAACCA CGCTGACCAG GGCAGGGACA ACTCTGAGAA CGACACTGTC 
CCCGAAGTCT TCCCAAGGTT GTTTCACCTG ATTCCAGATG GTGAAATTAC AAGCATCAAG 
ATCAATAGAG TGGATCCCAA TGAAAGCCTC TCCATAAGGC TTGTGGGAGG CAGCGAGACC 
CCGCTGGTCC ACATCATCAT CCAGCATATT TATCGTGATG GAGTGATTGC CAGAGATGGA 
CGACTGCTGC CAGGAGACAT CATTCTCAAG GTCAATGGCA TGGACATCAG CAATGTCCCT 
CATAACTATG CCGTGCGGCT CCTGCGGCAG CCCTGCCAGG TGCTACGGCT AACGGTGCTG 
CGGGAACAGA AGTTCCGTAG CAGAAGCAAT GCTCCTGCGC CGGACTCCTA CGGACCTCGG 
GATGACAGCT TCCATGTAAT CCTCAACAAA AGCAGCCCTG AGGAGCAGCT TGGCATAAAG 
CTGGTGCGCA GGGTGGATGA GCCCGGGGTG TTCATCTTCA ACGTGCTGAA TGGCGGTGTT 
GCCGACCGAC ACGGCCAGCT GGAGGAGAAT GATCGTGTGT TGGCCATCAA CGGACATGAC 
CTTCGATTTG GCAGTCCAGA GAGCGCAGCT CATCTGATTC AGGCCAGTGA AAGACGTGTT 
CACCTCGTCG TGTCCCGCCA GGTTCGACAG CCCAGCCCAG ACATCTTTCA AGAAGCTGGC 
TGCATCAGCA ACGGCCAGCA GTCTCCTGTC TCAGGGGAGA GGAGCACATC TTCTAAGCCT 
GCAGCCACTT GTCATGAGAA GGTTGTAAGT GTCCAAAAAG ACCCCAACGA ATCTCTCGGC 
ATGACCGTTG GAGGGGGAGC ATCACACAGA GAATGGGACT TGCCCATCTA TGTCATCAGT 
GTTGAGCCCG GAGGAGTCAT AAGCAGAGAC GGAAGAATAA AAACAGGTGA CATTTTATTG 
AATGTGAATG GGATTGAGCT CACAGAGGTC AGCCGGACAG AGGCCGTTGC CATACTGAAG 
AGCACACCCT CCTCAGTGGT ACTCAAAGCC TTGGAAGTCA AGGAACAGGA GACCCAGGAA 
GACTGCAGCC CAGCAGCCCT GGACTCCAAC CACAACGTGA CCCCGCCTGG TGACTGGTCC 
CCATCCTGGG TCATGTGGCT GGAATTACCA CAGTACTTAT ATAACTGTAA GGATGTGATA 
CTACGAAGGA ACACAGCGGG AAGCCTGGGC TTCTGCATTG TGGGAGGTTA TGAAGAATAC 
AGTGGTAACC AACCTTTTTT TATCAAATCC ATCGTCGAGG GAACACCCGC GTATAATGAT 
GGAAGAATTA GATGTGGTGA CATTCTTCTT GCTGTCAACG GCAGAAGCAC GTCGGGGATG 
ATGCACGCCT GCTTAGCGAG GATGCTCAAA GAACTCAAAG GGAAAATTAC TCTCACCATT 
GCTTCCTGGC CTGGTACTTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lnx1 ( XM_006250904.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250966.1 Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa36026
Clone ID Related Accession (Same CDS sequence)	XM_006250904.3
Accession Version	XM_006250904.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase LNX isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250904.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGC TGCTGCTGCT GGTCCTGCCC TGGCTGAGTC CAGCTAACTA CATTGACAAT 
GTGGGCAACC TGCACTTCCT GTACTCGGAA CTCTGCAAAG GGGCCTCCCA CTATGGCCTG 
ACCAAAGACA GGAAAAGACG CTCACAAGAT GGCTGCCCTG ATGGTTGCGC CAGCCTCACG 
GCAACGACGC TCTCCCCAGA GGTTTCTGCT GCTGCCACCA TCTCTTTAAT GACAGACGAG 
CCTGGCCTAG ACAATCCTGC CTACACGTCC TCAGTGGAGG ATGGTGAACC GGTAACCAAC 
CCCTCAGACA CTGGCCGGAG CAACCGAACC AGGGCACGGC CCTTTGAGCG CTCCACTATG 
AGAAGCCGCT CTTTCAAAAA GATCAACCGA GCCCTGAGTG CTCTCCGAAG GACGAAGAGT 
GGAAGTGCTG TTGCCAACCA CGCTGACCAG GGCAGGGACA ACTCTGAGAA CGACACTGTC 
CCCGAAGATG GTGAAATTAC AAGCATCAAG ATCAATAGAG TGGATCCCAA TGAAAGCCTC 
TCCATAAGGC TTGTGGGAGG CAGCGAGACC CCGCTGGTCC ACATCATCAT CCAGCATATT 
TATCGTGATG GAGTGATTGC CAGAGATGGA CGACTGCTGC CAGGAGACAT CATTCTCAAG 
GTCAATGGCA TGGACATCAG CAATGTCCCT CATAACTATG CCGTGCGGCT CCTGCGGCAG 
CCCTGCCAGG TGCTACGGCT AACGGTGCTG CGGGAACAGA AGTTCCGTAG CAGAAGCAAT 
GCTCCTGCGC CGGACTCCTA CGGACCTCGG GATGACAGCT TCCATGTAAT CCTCAACAAA 
AGCAGCCCTG AGGAGCAGCT TGGCATAAAG CTGGTGCGCA GGGTGGATGA GCCCGGGGTG 
TTCATCTTCA ACGTGCTGAA TGGCGGTGTT GCCGACCGAC ACGGCCAGCT GGAGGAGAAT 
GATCGTGTGT TGGCCATCAA CGGACATGAC CTTCGATTTG GCAGTCCAGA GAGCGCAGCT 
CATCTGATTC AGGCCAGTGA AAGACGTGTT CACCTCGTCG TGTCCCGCCA GGTTCGACAG 
CCCAGCCCAG ACATCTTTCA AGAAGCTGGC TGCATCAGCA ACGGCCAGCA GTCTCCTGTC 
TCAGGGGAGA GGAGCACATC TTCTAAGCCT GCAGCCACTT GTCATGAGAA GGTTGTAAGT 
GTCCAAAAAG ACCCCAACGA ATCTCTCGGC ATGACCGTTG GAGGGGGAGC ATCACACAGA 
GAATGGGACT TGCCCATCTA TGTCATCAGT GTTGAGCCCG GAGGAGTCAT AAGCAGAGAC 
GGAAGAATAA AAACAGGTGA CATTTTATTG AATGTGAATG GGATTGAGCT CACAGAGGTC 
AGCCGGACAG AGGCCGTTGC CATACTGAAG AGCACACCCT CCTCAGTGGT ACTCAAAGCC 
TTGGAAGTCA AGGAACAGGA GACCCAGGAA GACTGCAGCC CAGCAGCCCT GGACTCCAAC 
CACAACGTGA CCCCGCCTGG TGACTGGTCC CCATCCTGGG TCATGTGGCT GGAATTACCA 
CAGTACTTAT ATAACTGTAA GGATGTGATA CTACGAAGGA ACACAGCGGG AAGCCTGGGC 
TTCTGCATTG TGGGAGGTTA TGAAGAATAC AGTGGTAACC AACCTTTTTT TATCAAATCC 
ATCGTCGAGG GAACACCCGC GTATAATGAT GGAAGAATTA GATGTGGTGA CATTCTTCTT 
GCTGTCAACG GCAGAAGCAC GTCGGGGATG ATGCACGCCT GCTTAGCGAG GATGCTCAAA 
GAACTCAAAG GGAAAATTAC TCTCACCATT GCTTCCTGGC CTGGTACTTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250966.1
CDS	2524..4380
Translation	MKALLLLVLPWLSPANYIDNVGNLHFLYSELCKGASHYGLTKDRKRRSQDGCPDGCASLTATTLSPEVSAAATISLMTDEPGLDNPAYTSSVEDGEPVTNPSDTGRSNRTRARPFERSTMRSRSFKKINRALSALRRTKSGSAVANHADQGRDNSENDTVPEDGEITSIKINRVDPNESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQVLRLTVLREQKFRSRSNAPAPDSYGPRDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQPSPDIFQEAGCISNGQQSPVSGERSTSSKPAATCHEKVVSVQKDPNESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSTPSSVVLKALEVKEQETQEDCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLYNCKDVILRRNTAGSLGFCIVGGYEEYSGNQPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMMHACLARMLKELKGKITLTIASWPGTFL

Target ORF information:

RefSeq Version	XM_006250904.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250904.3

ORF Insert Sequence:

ATGAAGGCGC TGCTGCTGCT GGTCCTGCCC TGGCTGAGTC CAGCTAACTA CATTGACAAT 
GTGGGCAACC TGCACTTCCT GTACTCGGAA CTCTGCAAAG GGGCCTCCCA CTATGGCCTG 
ACCAAAGACA GGAAAAGACG CTCACAAGAT GGCTGCCCTG ATGGTTGCGC CAGCCTCACG 
GCAACGACGC TCTCCCCAGA GGTTTCTGCT GCTGCCACCA TCTCTTTAAT GACAGACGAG 
CCTGGCCTAG ACAATCCTGC CTACACGTCC TCAGTGGAGG ATGGTGAACC GGTAACCAAC 
CCCTCAGACA CTGGCCGGAG CAACCGAACC AGGGCACGGC CCTTTGAGCG CTCCACTATG 
AGAAGCCGCT CTTTCAAAAA GATCAACCGA GCCCTGAGTG CTCTCCGAAG GACGAAGAGT 
GGAAGTGCTG TTGCCAACCA CGCTGACCAG GGCAGGGACA ACTCTGAGAA CGACACTGTC 
CCCGAAGATG GTGAAATTAC AAGCATCAAG ATCAATAGAG TGGATCCCAA TGAAAGCCTC 
TCCATAAGGC TTGTGGGAGG CAGCGAGACC CCGCTGGTCC ACATCATCAT CCAGCATATT 
TATCGTGATG GAGTGATTGC CAGAGATGGA CGACTGCTGC CAGGAGACAT CATTCTCAAG 
GTCAATGGCA TGGACATCAG CAATGTCCCT CATAACTATG CCGTGCGGCT CCTGCGGCAG 
CCCTGCCAGG TGCTACGGCT AACGGTGCTG CGGGAACAGA AGTTCCGTAG CAGAAGCAAT 
GCTCCTGCGC CGGACTCCTA CGGACCTCGG GATGACAGCT TCCATGTAAT CCTCAACAAA 
AGCAGCCCTG AGGAGCAGCT TGGCATAAAG CTGGTGCGCA GGGTGGATGA GCCCGGGGTG 
TTCATCTTCA ACGTGCTGAA TGGCGGTGTT GCCGACCGAC ACGGCCAGCT GGAGGAGAAT 
GATCGTGTGT TGGCCATCAA CGGACATGAC CTTCGATTTG GCAGTCCAGA GAGCGCAGCT 
CATCTGATTC AGGCCAGTGA AAGACGTGTT CACCTCGTCG TGTCCCGCCA GGTTCGACAG 
CCCAGCCCAG ACATCTTTCA AGAAGCTGGC TGCATCAGCA ACGGCCAGCA GTCTCCTGTC 
TCAGGGGAGA GGAGCACATC TTCTAAGCCT GCAGCCACTT GTCATGAGAA GGTTGTAAGT 
GTCCAAAAAG ACCCCAACGA ATCTCTCGGC ATGACCGTTG GAGGGGGAGC ATCACACAGA 
GAATGGGACT TGCCCATCTA TGTCATCAGT GTTGAGCCCG GAGGAGTCAT AAGCAGAGAC 
GGAAGAATAA AAACAGGTGA CATTTTATTG AATGTGAATG GGATTGAGCT CACAGAGGTC 
AGCCGGACAG AGGCCGTTGC CATACTGAAG AGCACACCCT CCTCAGTGGT ACTCAAAGCC 
TTGGAAGTCA AGGAACAGGA GACCCAGGAA GACTGCAGCC CAGCAGCCCT GGACTCCAAC 
CACAACGTGA CCCCGCCTGG TGACTGGTCC CCATCCTGGG TCATGTGGCT GGAATTACCA 
CAGTACTTAT ATAACTGTAA GGATGTGATA CTACGAAGGA ACACAGCGGG AAGCCTGGGC 
TTCTGCATTG TGGGAGGTTA TGAAGAATAC AGTGGTAACC AACCTTTTTT TATCAAATCC 
ATCGTCGAGG GAACACCCGC GTATAATGAT GGAAGAATTA GATGTGGTGA CATTCTTCTT 
GCTGTCAACG GCAGAAGCAC GTCGGGGATG ATGCACGCCT GCTTAGCGAG GATGCTCAAA 
GAACTCAAAG GGAAAATTAC TCTCACCATT GCTTCCTGGC CTGGTACTTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lnx1 ( NM_001108358.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101828.1 Rattus norvegicus ligand of numb-protein X 1 (Lnx1), mRNA.

Price & Availability↑

CloneID	ORa07193
Clone ID Related Accession (Same CDS sequence)	NM_001108358.1
Accession Version	NM_001108358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase LNX
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473981.1. On or before Oct 4, 2007 this sequence version replaced XM_001067995.1, XM_001068098.1, XM_001068052.1, XM_001068150.1, XM_341204.3. ##Evidence-Data-START## CDS exon combination :: EU219404.1, EU219405.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAACCAAC CAGACCTTGC TGACGACCCA GATCCCAGCC CCGAGCCCCT GTGCGTAGTG 
TGTGGCCAAA ACCACTCCCC AGAGGAAAAC CACTTCTACA CATACACAGA AGATGTAGAC 
GACGACCTCG TATGCCACAT CTGCCTACAA GCATTGCTAG ACCCTCTGGA CACTCCGTGT 
GGACACACCT ACTGTACCCT GTGTCTCACC AACTTCCTGG TGGAGAAGGA CTTCTGCCCT 
GTGGATCGAA AGCCTGTGGT TCTGCAGCAC TGCAAGAAAT CCAACATCTT GGTCAACAAG 
CTTCTCAACA AGCTGTTGGT CAACTGCCCA TTCACAGAGC ACTGCTCCGA GGTGCTCCAG 
CGCTGCAATC TCCAGTATCA TTTCCAGACC AGCTGCAAAG GGGCCTCCCA CTATGGCCTG 
ACCAAAGACA GGAAAAGACG CTCACAAGAT GGCTGCCCTG ATGGTTGCGC CAGCCTCACG 
GCAACGACGC TCTCCCCAGA GGTTTCTGCT GCTGCCACCA TCTCTTTAAT GACAGACGAG 
CCTGGCCTAG ACAATCCTGC CTACACGTCC TCAGTGGAGG ATGGTGAACC GGTAACCAAC 
CCCTCAGACA CTGGCCGGAG CAACCGAACC AGGGCACGGC CCTTTGAGCG CTCCACTATG 
AGAAGCCGCT CTTTCAAAAA GATCAACCGA GCCCTGAGTG CTCTCCGAAG GACGAAGAGT 
GGAAGTGCTG TTGCCAACCA CGCTGACCAG GGCAGGGACA ACTCTGAGAA CGACACTGTC 
CCCGAAGTCT TCCCAAGGTT GTTTCACCTG ATTCCAGATG GTGAAATTAC AAGCATCAAG 
ATCAATAGAG TGGATCCCAA TGAAAGCCTC TCCATAAGGC TTGTGGGAGG CAGCGAGACC 
CCGCTGGTCC ACATCATCAT CCAGCATATT TATCGTGATG GAGTGATTGC CAGAGATGGA 
CGACTGCTGC CAGGAGACAT CATTCTCAAG GTCAATGGCA TGGACATCAG CAATGTCCCT 
CATAACTATG CCGTGCGGCT CCTGCGGCAG CCCTGCCAGG TGCTACGGCT AACGGTGCTG 
CGGGAACAGA AGTTCCGTAG CAGAAGCAAT GCTCCTGCGC CGGACTCCTA CGGACCTCGG 
GATGACAGCT TCCATGTAAT CCTCAACAAA AGCAGCCCTG AGGAGCAGCT TGGCATAAAG 
CTGGTGCGCA GGGTGGATGA GCCCGGGGTG TTCATCTTCA ACGTGCTGAA TGGCGGTGTT 
GCCGACCGAC ACGGCCAGCT GGAGGAGAAT GATCGTGTGT TGGCCATCAA CGGACATGAC 
CTTCGATTTG GCAGTCCAGA GAGCGCAGCT CATCTGATTC AGGCCAGTGA AAGACGTGTT 
CACCTCGTCG TGTCCCGCCA GGTTCGACAG CCCAGCCCAG ACATCTTTCA AGAAGCTGGC 
TGCATCAGCA ACGGCCAGCA GTCTCCTGTC TCAGGGGAGA GGAGCACATC TTCTAAGCCT 
GCAGCCACTT GTCATGAGAA GGTTGTAAGT GTCCAAAAAG ACCCCAACGA ATCTCTCGGC 
ATGACCGTTG GAGGGGGAGC ATCACACAGA GAATGGGACT TGCCCATCTA TGTCATCAGT 
GTTGAGCCCG GAGGAGTCAT AAGCAGAGAC GGAAGAATAA AAACAGGTGA CATTTTATTG 
AATGTGAATG GGATTGAGCT CACAGAGGTC AGCCGGACAG AGGCCGTTGC CATACTGAAG 
AGCACACCCT CCTCAGTGGT ACTCAAAGCC TTGGAAGTCA AGGAACAGGA GACCCAGGAA 
GACTGCAGCC CAGCAGCCCT GGACTCCAAC CACAACGTGA CCCCGCCTGG TGACTGGTCC 
CCATCCTGGG TCATGTGGCT GGAATTACCA CAGTACTTAT ATAACTGTAA GGATGTGATA 
CTACGAAGGA ACACAGCGGG AAGCCTGGGC TTCTGCATTG TGGGAGGTTA TGAAGAATAC 
AGTGGTAACC AACCTTTTTT TATCAAATCC ATCGTCGAGG GAACACCCGC GTATAATGAT 
GGAAGAATTA GATGTGGTGA CATTCTTCTT GCTGTCAACG GCAGAAGCAC GTCGGGGATG 
ATGCACGCCT GCTTAGCGAG GATGCTCAAA GAACTCAAAG GGAAAATTAC TCTCACCATT 
GCTTCCTGGC CTGGTACTTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101828.1
CDS	191..2377
Translation	MNQPDLADDPDPSPEPLCVVCGQNHSPEENHFYTYTEDVDDDLVCHICLQALLDPLDTPCGHTYCTLCLTNFLVEKDFCPVDRKPVVLQHCKKSNILVNKLLNKLLVNCPFTEHCSEVLQRCNLQYHFQTSCKGASHYGLTKDRKRRSQDGCPDGCASLTATTLSPEVSAAATISLMTDEPGLDNPAYTSSVEDGEPVTNPSDTGRSNRTRARPFERSTMRSRSFKKINRALSALRRTKSGSAVANHADQGRDNSENDTVPEVFPRLFHLIPDGEITSIKINRVDPNESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQVLRLTVLREQKFRSRSNAPAPDSYGPRDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQPSPDIFQEAGCISNGQQSPVSGERSTSSKPAATCHEKVVSVQKDPNESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSTPSSVVLKALEVKEQETQEDCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLYNCKDVILRRNTAGSLGFCIVGGYEEYSGNQPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMMHACLARMLKELKGKITLTIASWPGTFL

Target ORF information:

RefSeq Version	NM_001108358.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ligand of numb-protein X 1 (Lnx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108358.1

ORF Insert Sequence:

ATGAACCAAC CAGACCTTGC TGACGACCCA GATCCCAGCC CCGAGCCCCT GTGCGTAGTG 
TGTGGCCAAA ACCACTCCCC AGAGGAAAAC CACTTCTACA CATACACAGA AGATGTAGAC 
GACGACCTCG TATGCCACAT CTGCCTACAA GCATTGCTAG ACCCTCTGGA CACTCCGTGT 
GGACACACCT ACTGTACCCT GTGTCTCACC AACTTCCTGG TGGAGAAGGA CTTCTGCCCT 
GTGGATCGAA AGCCTGTGGT TCTGCAGCAC TGCAAGAAAT CCAACATCTT GGTCAACAAG 
CTTCTCAACA AGCTGTTGGT CAACTGCCCA TTCACAGAGC ACTGCTCCGA GGTGCTCCAG 
CGCTGCAATC TCCAGTATCA TTTCCAGACC AGCTGCAAAG GGGCCTCCCA CTATGGCCTG 
ACCAAAGACA GGAAAAGACG CTCACAAGAT GGCTGCCCTG ATGGTTGCGC CAGCCTCACG 
GCAACGACGC TCTCCCCAGA GGTTTCTGCT GCTGCCACCA TCTCTTTAAT GACAGACGAG 
CCTGGCCTAG ACAATCCTGC CTACACGTCC TCAGTGGAGG ATGGTGAACC GGTAACCAAC 
CCCTCAGACA CTGGCCGGAG CAACCGAACC AGGGCACGGC CCTTTGAGCG CTCCACTATG 
AGAAGCCGCT CTTTCAAAAA GATCAACCGA GCCCTGAGTG CTCTCCGAAG GACGAAGAGT 
GGAAGTGCTG TTGCCAACCA CGCTGACCAG GGCAGGGACA ACTCTGAGAA CGACACTGTC 
CCCGAAGTCT TCCCAAGGTT GTTTCACCTG ATTCCAGATG GTGAAATTAC AAGCATCAAG 
ATCAATAGAG TGGATCCCAA TGAAAGCCTC TCCATAAGGC TTGTGGGAGG CAGCGAGACC 
CCGCTGGTCC ACATCATCAT CCAGCATATT TATCGTGATG GAGTGATTGC CAGAGATGGA 
CGACTGCTGC CAGGAGACAT CATTCTCAAG GTCAATGGCA TGGACATCAG CAATGTCCCT 
CATAACTATG CCGTGCGGCT CCTGCGGCAG CCCTGCCAGG TGCTACGGCT AACGGTGCTG 
CGGGAACAGA AGTTCCGTAG CAGAAGCAAT GCTCCTGCGC CGGACTCCTA CGGACCTCGG 
GATGACAGCT TCCATGTAAT CCTCAACAAA AGCAGCCCTG AGGAGCAGCT TGGCATAAAG 
CTGGTGCGCA GGGTGGATGA GCCCGGGGTG TTCATCTTCA ACGTGCTGAA TGGCGGTGTT 
GCCGACCGAC ACGGCCAGCT GGAGGAGAAT GATCGTGTGT TGGCCATCAA CGGACATGAC 
CTTCGATTTG GCAGTCCAGA GAGCGCAGCT CATCTGATTC AGGCCAGTGA AAGACGTGTT 
CACCTCGTCG TGTCCCGCCA GGTTCGACAG CCCAGCCCAG ACATCTTTCA AGAAGCTGGC 
TGCATCAGCA ACGGCCAGCA GTCTCCTGTC TCAGGGGAGA GGAGCACATC TTCTAAGCCT 
GCAGCCACTT GTCATGAGAA GGTTGTAAGT GTCCAAAAAG ACCCCAACGA ATCTCTCGGC 
ATGACCGTTG GAGGGGGAGC ATCACACAGA GAATGGGACT TGCCCATCTA TGTCATCAGT 
GTTGAGCCCG GAGGAGTCAT AAGCAGAGAC GGAAGAATAA AAACAGGTGA CATTTTATTG 
AATGTGAATG GGATTGAGCT CACAGAGGTC AGCCGGACAG AGGCCGTTGC CATACTGAAG 
AGCACACCCT CCTCAGTGGT ACTCAAAGCC TTGGAAGTCA AGGAACAGGA GACCCAGGAA 
GACTGCAGCC CAGCAGCCCT GGACTCCAAC CACAACGTGA CCCCGCCTGG TGACTGGTCC 
CCATCCTGGG TCATGTGGCT GGAATTACCA CAGTACTTAT ATAACTGTAA GGATGTGATA 
CTACGAAGGA ACACAGCGGG AAGCCTGGGC TTCTGCATTG TGGGAGGTTA TGAAGAATAC 
AGTGGTAACC AACCTTTTTT TATCAAATCC ATCGTCGAGG GAACACCCGC GTATAATGAT 
GGAAGAATTA GATGTGGTGA CATTCTTCTT GCTGTCAACG GCAGAAGCAC GTCGGGGATG 
ATGCACGCCT GCTTAGCGAG GATGCTCAAA GAACTCAAAG GGAAAATTAC TCTCACCATT 
GCTTCCTGGC CTGGTACTTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lnx1 ( XM_006250902.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006250964.1 Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X2, mRNA.

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CloneID	ORa36024
Clone ID Related Accession (Same CDS sequence)	XM_006250902.3
Accession Version	XM_006250902.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2157bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase LNX isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006250902.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAAC CAGACCTTGC TGACGACCCA GATCCCAGCC CCGAGCCCCT GTGCGTAGTG 
TGTGGCCAAA ACCACTCCCC AGAGGAAAAC CACTTCTACA CATACACAGA AGATGTAGAC 
GACGACCTCG TATGCCACAT CTGCCTACAA GCATTGCTGG ACCCTCTGGA CACTCCGTGT 
GGACACACCT ACTGTACCCT GTGTCTCACC AACTTCCTGG TGGAGAAGGA CTTCTGCCCT 
GTGGATCGAA AGCCTGTGGT TCTGCAGCAC TGCAAGAAAT CCAACATCTT GGTCAACAAG 
CTTCTCAACA AGCTGTTGGT CAACTGCCCA TTCACAGAGC ACTGCTCCGA GGTGCTCCAG 
CGCTGCAATC TCCAGTATCA TTTCCAGACC AGCTGCAAAG GGGCCTCCCA CTATGGCCTG 
ACCAAAGACA GGAAAAGACG CTCACAAGAT GGCTGCCCTG ATGGTTGCGC CAGCCTCACG 
GCAACGACGC TCTCCCCAGA GGTTTCTGCT GCTGCCACCA TCTCTTTAAT GACAGACGAG 
CCTGGCCTAG ACAATCCTGC CTACACGTCC TCAGTGGAGG ATGGTGAACC GGTAACCAAC 
CCCTCAGACA CTGGCCGGAG CAACCGAACC AGGGCACGGC CCTTTGAGCG CTCCACTATG 
AGAAGCCGCT CTTTCAAAAA GATCAACCGA GCCCTGAGTG CTCTCCGAAG GACGAAGAGT 
GGAAGTGCTG TTGCCAACCA CGCTGACCAG GGCAGGGACA ACTCTGAGAA CGACACTGTC 
CCCGAAGATG GTGAAATTAC AAGCATCAAG ATCAATAGAG TGGATCCCAA TGAAAGCCTC 
TCCATAAGGC TTGTGGGAGG CAGCGAGACC CCGCTGGTCC ACATCATCAT CCAGCATATT 
TATCGTGATG GAGTGATTGC CAGAGATGGA CGACTGCTGC CAGGAGACAT CATTCTCAAG 
GTCAATGGCA TGGACATCAG CAATGTCCCT CATAACTATG CCGTGCGGCT CCTGCGGCAG 
CCCTGCCAGG TGCTACGGCT AACGGTGCTG CGGGAACAGA AGTTCCGTAG CAGAAGCAAT 
GCTCCTGCGC CGGACTCCTA CGGACCTCGG GATGACAGCT TCCATGTAAT CCTCAACAAA 
AGCAGCCCTG AGGAGCAGCT TGGCATAAAG CTGGTGCGCA GGGTGGATGA GCCCGGGGTG 
TTCATCTTCA ACGTGCTGAA TGGCGGTGTT GCCGACCGAC ACGGCCAGCT GGAGGAGAAT 
GATCGTGTGT TGGCCATCAA CGGACATGAC CTTCGATTTG GCAGTCCAGA GAGCGCAGCT 
CATCTGATTC AGGCCAGTGA AAGACGTGTT CACCTCGTCG TGTCCCGCCA GGTTCGACAG 
CCCAGCCCAG ACATCTTTCA AGAAGCTGGC TGCATCAGCA ACGGCCAGCA GTCTCCTGTC 
TCAGGGGAGA GGAGCACATC TTCTAAGCCT GCAGCCACTT GTCATGAGAA GGTTGTAAGT 
GTCCAAAAAG ACCCCAACGA ATCTCTCGGC ATGACCGTTG GAGGGGGAGC ATCACACAGA 
GAATGGGACT TGCCCATCTA TGTCATCAGT GTTGAGCCCG GAGGAGTCAT AAGCAGAGAC 
GGAAGAATAA AAACAGGTGA CATTTTATTG AATGTGAATG GGATTGAGCT CACAGAGGTC 
AGCCGGACAG AGGCCGTTGC CATACTGAAG AGCACACCCT CCTCAGTGGT ACTCAAAGCC 
TTGGAAGTCA AGGAACAGGA GACCCAGGAA GACTGCAGCC CAGCAGCCCT GGACTCCAAC 
CACAACGTGA CCCCGCCTGG TGACTGGTCC CCATCCTGGG TCATGTGGCT GGAATTACCA 
CAGTACTTAT ATAACTGTAA GGATGTGATA CTACGAAGGA ACACAGCGGG AAGCCTGGGC 
TTCTGCATTG TGGGAGGTTA TGAAGAATAC AGTGGTAACC AACCTTTTTT TATCAAATCC 
ATCGTCGAGG GAACACCCGC GTATAATGAT GGAAGAATTA GATGTGGTGA CATTCTTCTT 
GCTGTCAACG GCAGAAGCAC GTCGGGGATG ATGCACGCCT GCTTAGCGAG GATGCTCAAA 
GAACTCAAAG GGAAAATTAC TCTCACCATT GCTTCCTGGC CTGGTACTTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006250964.1
CDS	199..2355
Translation	MNQPDLADDPDPSPEPLCVVCGQNHSPEENHFYTYTEDVDDDLVCHICLQALLDPLDTPCGHTYCTLCLTNFLVEKDFCPVDRKPVVLQHCKKSNILVNKLLNKLLVNCPFTEHCSEVLQRCNLQYHFQTSCKGASHYGLTKDRKRRSQDGCPDGCASLTATTLSPEVSAAATISLMTDEPGLDNPAYTSSVEDGEPVTNPSDTGRSNRTRARPFERSTMRSRSFKKINRALSALRRTKSGSAVANHADQGRDNSENDTVPEDGEITSIKINRVDPNESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQVLRLTVLREQKFRSRSNAPAPDSYGPRDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQPSPDIFQEAGCISNGQQSPVSGERSTSSKPAATCHEKVVSVQKDPNESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSTPSSVVLKALEVKEQETQEDCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLYNCKDVILRRNTAGSLGFCIVGGYEEYSGNQPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMMHACLARMLKELKGKITLTIASWPGTFL

Target ORF information:

RefSeq Version	XM_006250902.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006250902.3

ORF Insert Sequence:

ATGAACCAAC CAGACCTTGC TGACGACCCA GATCCCAGCC CCGAGCCCCT GTGCGTAGTG 
TGTGGCCAAA ACCACTCCCC AGAGGAAAAC CACTTCTACA CATACACAGA AGATGTAGAC 
GACGACCTCG TATGCCACAT CTGCCTACAA GCATTGCTGG ACCCTCTGGA CACTCCGTGT 
GGACACACCT ACTGTACCCT GTGTCTCACC AACTTCCTGG TGGAGAAGGA CTTCTGCCCT 
GTGGATCGAA AGCCTGTGGT TCTGCAGCAC TGCAAGAAAT CCAACATCTT GGTCAACAAG 
CTTCTCAACA AGCTGTTGGT CAACTGCCCA TTCACAGAGC ACTGCTCCGA GGTGCTCCAG 
CGCTGCAATC TCCAGTATCA TTTCCAGACC AGCTGCAAAG GGGCCTCCCA CTATGGCCTG 
ACCAAAGACA GGAAAAGACG CTCACAAGAT GGCTGCCCTG ATGGTTGCGC CAGCCTCACG 
GCAACGACGC TCTCCCCAGA GGTTTCTGCT GCTGCCACCA TCTCTTTAAT GACAGACGAG 
CCTGGCCTAG ACAATCCTGC CTACACGTCC TCAGTGGAGG ATGGTGAACC GGTAACCAAC 
CCCTCAGACA CTGGCCGGAG CAACCGAACC AGGGCACGGC CCTTTGAGCG CTCCACTATG 
AGAAGCCGCT CTTTCAAAAA GATCAACCGA GCCCTGAGTG CTCTCCGAAG GACGAAGAGT 
GGAAGTGCTG TTGCCAACCA CGCTGACCAG GGCAGGGACA ACTCTGAGAA CGACACTGTC 
CCCGAAGATG GTGAAATTAC AAGCATCAAG ATCAATAGAG TGGATCCCAA TGAAAGCCTC 
TCCATAAGGC TTGTGGGAGG CAGCGAGACC CCGCTGGTCC ACATCATCAT CCAGCATATT 
TATCGTGATG GAGTGATTGC CAGAGATGGA CGACTGCTGC CAGGAGACAT CATTCTCAAG 
GTCAATGGCA TGGACATCAG CAATGTCCCT CATAACTATG CCGTGCGGCT CCTGCGGCAG 
CCCTGCCAGG TGCTACGGCT AACGGTGCTG CGGGAACAGA AGTTCCGTAG CAGAAGCAAT 
GCTCCTGCGC CGGACTCCTA CGGACCTCGG GATGACAGCT TCCATGTAAT CCTCAACAAA 
AGCAGCCCTG AGGAGCAGCT TGGCATAAAG CTGGTGCGCA GGGTGGATGA GCCCGGGGTG 
TTCATCTTCA ACGTGCTGAA TGGCGGTGTT GCCGACCGAC ACGGCCAGCT GGAGGAGAAT 
GATCGTGTGT TGGCCATCAA CGGACATGAC CTTCGATTTG GCAGTCCAGA GAGCGCAGCT 
CATCTGATTC AGGCCAGTGA AAGACGTGTT CACCTCGTCG TGTCCCGCCA GGTTCGACAG 
CCCAGCCCAG ACATCTTTCA AGAAGCTGGC TGCATCAGCA ACGGCCAGCA GTCTCCTGTC 
TCAGGGGAGA GGAGCACATC TTCTAAGCCT GCAGCCACTT GTCATGAGAA GGTTGTAAGT 
GTCCAAAAAG ACCCCAACGA ATCTCTCGGC ATGACCGTTG GAGGGGGAGC ATCACACAGA 
GAATGGGACT TGCCCATCTA TGTCATCAGT GTTGAGCCCG GAGGAGTCAT AAGCAGAGAC 
GGAAGAATAA AAACAGGTGA CATTTTATTG AATGTGAATG GGATTGAGCT CACAGAGGTC 
AGCCGGACAG AGGCCGTTGC CATACTGAAG AGCACACCCT CCTCAGTGGT ACTCAAAGCC 
TTGGAAGTCA AGGAACAGGA GACCCAGGAA GACTGCAGCC CAGCAGCCCT GGACTCCAAC 
CACAACGTGA CCCCGCCTGG TGACTGGTCC CCATCCTGGG TCATGTGGCT GGAATTACCA 
CAGTACTTAT ATAACTGTAA GGATGTGATA CTACGAAGGA ACACAGCGGG AAGCCTGGGC 
TTCTGCATTG TGGGAGGTTA TGAAGAATAC AGTGGTAACC AACCTTTTTT TATCAAATCC 
ATCGTCGAGG GAACACCCGC GTATAATGAT GGAAGAATTA GATGTGGTGA CATTCTTCTT 
GCTGTCAACG GCAGAAGCAC GTCGGGGATG ATGCACGCCT GCTTAGCGAG GATGCTCAAA 
GAACTCAAAG GGAAAATTAC TCTCACCATT GCTTCCTGGC CTGGTACTTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lnx1 ( XM_017599287.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454776.1 Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X1, mRNA.

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CloneID	ORa58358
Clone ID Related Accession (Same CDS sequence)	XM_017599287.1
Accession Version	XM_017599287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase LNX isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCAAC CAGACCTTGC TGACGACCCA GATCCCAGCC CCGAGCCCCT GTGCGTAGTG 
TGTGGCCAAA ACCACTCCCC AGAGGAAAAC CACTTCTACA CATACACAGA AGATGTAGAC 
GACGACCTCG TATGCCACAT CTGCCTACAA GCATTGCTGG ACCCTCTGGA CACTCCGTGT 
GGACACACCT ACTGTACCCT GTGTCTCACC AACTTCCTGG TGGAGAAGGA CTTCTGCCCT 
GTGGATCGAA AGCCTGTGGT TCTGCAGCAC TGCAAGAAAT CCAACATCTT GGTCAACAAG 
CTTCTCAACA AGCTGTTGGT CAACTGCCCA TTCACAGAGC ACTGCTCCGA GGTGCTCCAG 
CGCTGCAATC TCCAGTATCA TTTCCAGACC AGCTGCAAAG GGGCCTCCCA CTATGGCCTG 
ACCAAAGACA GGAAAAGACG CTCACAAGAT GGCTGCCCTG ATGGTTGCGC CAGCCTCACG 
GCAACGACGC TCTCCCCAGA GGTTTCTGCT GCTGCCACCA TCTCTTTAAT GACAGACGAG 
CCTGGCCTAG ACAATCCTGC CTACACGTCC TCAGTGGAGG ATGGTGAACC GGTAACCAAC 
CCCTCAGACA CTGGCCGGAG CAACCGAACC AGGGCACGGC CCTTTGAGCG CTCCACTATG 
AGAAGCCGCT CTTTCAAAAA GATCAACCGA GCCCTGAGTG CTCTCCGAAG GACGAAGAGT 
GGAAGTGCTG TTGCCAACCA CGCTGACCAG GGCAGGGACA ACTCTGAGAA CGACACTGTC 
CCCGAAGTCT TCCCAAGGTT GTTTCACCTG ATTCCAGATG GTGAAATTAC AAGCATCAAG 
ATCAATAGAG TGGATCCCAA TGAAAGCCTC TCCATAAGGC TTGTGGGAGG CAGCGAGACC 
CCGCTGGTCC ACATCATCAT CCAGCATATT TATCGTGATG GAGTGATTGC CAGAGATGGA 
CGACTGCTGC CAGGAGACAT CATTCTCAAG GTCAATGGCA TGGACATCAG CAATGTCCCT 
CATAACTATG CCGTGCGGCT CCTGCGGCAG CCCTGCCAGG TGCTACGGCT AACGGTGCTG 
CGGGAACAGA AGTTCCGTAG CAGAAGCAAT GCTCCTGCGC CGGACTCCTA CGGACCTCGG 
GATGACAGCT TCCATGTAAT CCTCAACAAA AGCAGCCCTG AGGAGCAGCT TGGCATAAAG 
CTGGTGCGCA GGGTGGATGA GCCCGGGGTG TTCATCTTCA ACGTGCTGAA TGGCGGTGTT 
GCCGACCGAC ACGGCCAGCT GGAGGAGAAT GATCGTGTGT TGGCCATCAA CGGACATGAC 
CTTCGATTTG GCAGTCCAGA GAGCGCAGCT CATCTGATTC AGGCCAGTGA AAGACGTGTT 
CACCTCGTCG TGTCCCGCCA GGTTCGACAG CCCAGCCCAG ACATCTTTCA AGAAGCTGGC 
TGCATCAGCA ACGGCCAGCA GTCTCCTGTC TCAGGGGAGA GGAGCACATC TTCTAAGCCT 
GCAGCCACTT GTCATGAGAA GGTTGTAAGT GTCCAAAAAG ACCCCAACGA ATCTCTCGGC 
ATGACCGTTG GAGGGGGAGC ATCACACAGA GAATGGGACT TGCCCATCTA TGTCATCAGT 
GTTGAGCCCG GAGGAGTCAT AAGCAGAGAC GGAAGAATAA AAACAGGTGA CATTTTATTG 
AATGTGAATG GGATTGAGCT CACAGAGGTC AGCCGGACAG AGGCCGTTGC CATACTGAAG 
AGCACACCCT CCTCAGTGGT ACTCAAAGCC TTGGAAGTCA AGGAACAGGA GACCCAGGAA 
GACTGCAGCC CAGCAGCCCT GGACTCCAAC CACAACGTGA CCCCGCCTGG TGACTGGTCC 
CCATCCTGGG TCATGTGGCT GGAATTACCA CAGTACTTAT ATAACTGTAA GGATGTGATA 
CTACGAAGGA ACACAGCGGG AAGCCTGGGC TTCTGCATTG TGGGAGGTTA TGAAGAATAC 
AGTGGTAACC AACCTTTTTT TATCAAATCC ATCGTCGAGG GAACACCCGC GTATAATGAT 
GGAAGAATTA GATGTGGTGA CATTCTTCTT GCTGTCAACG GCAGAAGCAC GTCGGGGATG 
ATGCACGCCT GCTTAGCGAG GATGCTCAAA GAACTCAAAG GGAAAATTAC TCTCACCATT 
GCTTCCTGGC CTGGTACTTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454776.1
CDS	702..2888
Translation	MNQPDLADDPDPSPEPLCVVCGQNHSPEENHFYTYTEDVDDDLVCHICLQALLDPLDTPCGHTYCTLCLTNFLVEKDFCPVDRKPVVLQHCKKSNILVNKLLNKLLVNCPFTEHCSEVLQRCNLQYHFQTSCKGASHYGLTKDRKRRSQDGCPDGCASLTATTLSPEVSAAATISLMTDEPGLDNPAYTSSVEDGEPVTNPSDTGRSNRTRARPFERSTMRSRSFKKINRALSALRRTKSGSAVANHADQGRDNSENDTVPEVFPRLFHLIPDGEITSIKINRVDPNESLSIRLVGGSETPLVHIIIQHIYRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQVLRLTVLREQKFRSRSNAPAPDSYGPRDDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAAHLIQASERRVHLVVSRQVRQPSPDIFQEAGCISNGQQSPVSGERSTSSKPAATCHEKVVSVQKDPNESLGMTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSTPSSVVLKALEVKEQETQEDCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLYNCKDVILRRNTAGSLGFCIVGGYEEYSGNQPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMMHACLARMLKELKGKITLTIASWPGTFL

Target ORF information:

RefSeq Version	XM_017599287.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ligand of numb-protein X 1 (Lnx1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599287.1

ORF Insert Sequence:

ATGAACCAAC CAGACCTTGC TGACGACCCA GATCCCAGCC CCGAGCCCCT GTGCGTAGTG 
TGTGGCCAAA ACCACTCCCC AGAGGAAAAC CACTTCTACA CATACACAGA AGATGTAGAC 
GACGACCTCG TATGCCACAT CTGCCTACAA GCATTGCTGG ACCCTCTGGA CACTCCGTGT 
GGACACACCT ACTGTACCCT GTGTCTCACC AACTTCCTGG TGGAGAAGGA CTTCTGCCCT 
GTGGATCGAA AGCCTGTGGT TCTGCAGCAC TGCAAGAAAT CCAACATCTT GGTCAACAAG 
CTTCTCAACA AGCTGTTGGT CAACTGCCCA TTCACAGAGC ACTGCTCCGA GGTGCTCCAG 
CGCTGCAATC TCCAGTATCA TTTCCAGACC AGCTGCAAAG GGGCCTCCCA CTATGGCCTG 
ACCAAAGACA GGAAAAGACG CTCACAAGAT GGCTGCCCTG ATGGTTGCGC CAGCCTCACG 
GCAACGACGC TCTCCCCAGA GGTTTCTGCT GCTGCCACCA TCTCTTTAAT GACAGACGAG 
CCTGGCCTAG ACAATCCTGC CTACACGTCC TCAGTGGAGG ATGGTGAACC GGTAACCAAC 
CCCTCAGACA CTGGCCGGAG CAACCGAACC AGGGCACGGC CCTTTGAGCG CTCCACTATG 
AGAAGCCGCT CTTTCAAAAA GATCAACCGA GCCCTGAGTG CTCTCCGAAG GACGAAGAGT 
GGAAGTGCTG TTGCCAACCA CGCTGACCAG GGCAGGGACA ACTCTGAGAA CGACACTGTC 
CCCGAAGTCT TCCCAAGGTT GTTTCACCTG ATTCCAGATG GTGAAATTAC AAGCATCAAG 
ATCAATAGAG TGGATCCCAA TGAAAGCCTC TCCATAAGGC TTGTGGGAGG CAGCGAGACC 
CCGCTGGTCC ACATCATCAT CCAGCATATT TATCGTGATG GAGTGATTGC CAGAGATGGA 
CGACTGCTGC CAGGAGACAT CATTCTCAAG GTCAATGGCA TGGACATCAG CAATGTCCCT 
CATAACTATG CCGTGCGGCT CCTGCGGCAG CCCTGCCAGG TGCTACGGCT AACGGTGCTG 
CGGGAACAGA AGTTCCGTAG CAGAAGCAAT GCTCCTGCGC CGGACTCCTA CGGACCTCGG 
GATGACAGCT TCCATGTAAT CCTCAACAAA AGCAGCCCTG AGGAGCAGCT TGGCATAAAG 
CTGGTGCGCA GGGTGGATGA GCCCGGGGTG TTCATCTTCA ACGTGCTGAA TGGCGGTGTT 
GCCGACCGAC ACGGCCAGCT GGAGGAGAAT GATCGTGTGT TGGCCATCAA CGGACATGAC 
CTTCGATTTG GCAGTCCAGA GAGCGCAGCT CATCTGATTC AGGCCAGTGA AAGACGTGTT 
CACCTCGTCG TGTCCCGCCA GGTTCGACAG CCCAGCCCAG ACATCTTTCA AGAAGCTGGC 
TGCATCAGCA ACGGCCAGCA GTCTCCTGTC TCAGGGGAGA GGAGCACATC TTCTAAGCCT 
GCAGCCACTT GTCATGAGAA GGTTGTAAGT GTCCAAAAAG ACCCCAACGA ATCTCTCGGC 
ATGACCGTTG GAGGGGGAGC ATCACACAGA GAATGGGACT TGCCCATCTA TGTCATCAGT 
GTTGAGCCCG GAGGAGTCAT AAGCAGAGAC GGAAGAATAA AAACAGGTGA CATTTTATTG 
AATGTGAATG GGATTGAGCT CACAGAGGTC AGCCGGACAG AGGCCGTTGC CATACTGAAG 
AGCACACCCT CCTCAGTGGT ACTCAAAGCC TTGGAAGTCA AGGAACAGGA GACCCAGGAA 
GACTGCAGCC CAGCAGCCCT GGACTCCAAC CACAACGTGA CCCCGCCTGG TGACTGGTCC 
CCATCCTGGG TCATGTGGCT GGAATTACCA CAGTACTTAT ATAACTGTAA GGATGTGATA 
CTACGAAGGA ACACAGCGGG AAGCCTGGGC TTCTGCATTG TGGGAGGTTA TGAAGAATAC 
AGTGGTAACC AACCTTTTTT TATCAAATCC ATCGTCGAGG GAACACCCGC GTATAATGAT 
GGAAGAATTA GATGTGGTGA CATTCTTCTT GCTGTCAACG GCAGAAGCAC GTCGGGGATG 
ATGCACGCCT GCTTAGCGAG GATGCTCAAA GAACTCAAAG GGAAAATTAC TCTCACCATT 
GCTTCCTGGC CTGGTACTTT TTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

LNX functions as a RING type E3 ubiquitin ligase that targets the cell fate determinant Numb for ubiquitin-dependent degradation.
The EMBO journal21(1-2)93-102(2002 Jan)
Nie J,McGill MA,Dermer M,Dho SE,Wolting CD,McGlade CJ

The Lnx family proteins function as molecular scaffolds for Numb family proteins.
Molecular and cellular neurosciences18(5)525-40(2001 Nov)
Rice DS,Northcutt GM,Kurschner C

The mammalian numb phosphotyrosine-binding domain. Characterization of binding specificity and identification of a novel PDZ domain-containing numb binding protein, LNX.
The Journal of biological chemistry273(15)9179-87(1998 Apr)
Dho SE,Jacob S,Wolting CD,French MB,Rohrschneider LR,McGlade CJ

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Lnx1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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