Gpbar1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpbar1
Entrez Gene ID	338443
Full Name	G protein-coupled bile acid receptor 1
Synonyms	Tgr5
General protein information	Preferred Names G protein-coupled bile acid receptor 1 Names G-protein coupled bile acid receptor 1 G protein-coupled receptor M-BAR membrane-type receptor for bile acids
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q33
Summary	a bile acid receptor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006245235.3	XP_006245297.1	G-protein coupled bile acid receptor 1 isoform X1
NM_177936.1	NP_808797.1	G-protein coupled bile acid receptor 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GPBAR1	XP_001155488.1
Mus musculus (house mouse)	Gpbar1	NP_778150.1
Homo sapiens (human)	GPBAR1	NP_001070659.1
Canis lupus familiaris (dog)	GPBAR1	XP_005640698.1
Bos taurus (cattle)	GPBAR1	NP_778219.1
	GPBAR1	XP_001088918.1
Rattus norvegicus (Norway rat)	Gpbar1	NP_808797.1

Gene Ontology
Bibliography

Related articles in PubMed

TGR5 contributes to hepatic cystogenesis in rodents with polycystic liver diseases through cyclic adenosine monophosphate/G?s signaling.
Masyuk TV, Masyuk AI, Lorenzo Pisarello M, Howard BN, Huang BQ, Lee PY, Fung X, Sergienko E, Ardecky RJ, Chung TDY, Pinkerton AB, LaRusso NF
Hepatology (Baltimore, Md.)66(4)1197-1218(2017 10)

The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance.
Yan X, Li P, Tang Z, Feng B
BMC endocrine disorders17(1)60(2017 Sep)

TUDCA: An Agonist of the Bile Acid Receptor GPBAR1/TGR5 With Anti-Inflammatory Effects in Microglial Cells.
Yanguas-Cas?s N, Barreda-Manso MA, Nieto-Sampedro M, Romero-Ram?rez L
Journal of cellular physiology232(8)2231-2245(2017 Aug)

Bile acid receptor TGR5 overexpression is associated with decreased intestinal mucosal injury and epithelial cell proliferation in obstructive jaundice.
Ji CG, Xie XL, Yin J, Qi W, Chen L, Bai Y, Wang N, Zhao DQ, Jiang XY, Jiang HQ
Translational research : the journal of laboratory and clinical medicine18288-102(2017 Apr)

TGR5 suppresses high glucose-induced upregulation of fibronectin and transforming growth factor-?1 in rat glomerular mesangial cells by inhibiting RhoA/ROCK signaling.
Xiong F, Li X, Yang Z, Wang Y, Gong W, Huang J, Chen C, Liu P, Huang H
Endocrine54(3)657-670(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

contributes to hepatic cystogenesis by increasing cAMP and enhancing cholangiocyte proliferation
Title: TGR5 contributes to hepatic cystogenesis in rodents with polycystic liver diseases through cyclic adenosine monophosphate/G?s signaling.

GPBAR1/TGR5 receptor agonist, tauroursodeoxycholic acid, has anti-inflammatory effects in microglial cells.
Title: TUDCA: An Agonist of the Bile Acid Receptor GPBAR1/TGR5 With Anti-Inflammatory Effects in Microglial Cells.

bile acids promote intestinal epithelial cell proliferation and decrease mucosal injury by upregulating TGR5 expression in obstructive jaundice.
Title: Bile acid receptor TGR5 overexpression is associated with decreased intestinal mucosal injury and epithelial cell proliferation in obstructive jaundice.

In high glucose-treated glomerular mesangial cells, TGR5 can suppress the NF-kappaB-mediated upregulation of fibronectin and TGF-beta1 by inhibiting RhoA/ROCK signaling via protein kinase A.
Title: TGR5 suppresses high glucose-induced upregulation of fibronectin and transforming growth factor-?1 in rat glomerular mesangial cells by inhibiting RhoA/ROCK signaling.

TGR5 activation reduced ICAM-1, TGF-beta1 and FN expressions induced by high glucose in glomerular mesangial cells, the mechanism might be through suppressing S1P/S1P2 signaling, thus ameliorating diabetic nephropathy.
Title: TGR5 activation suppressed S1P/S1P2 signaling and resisted high glucose-induced fibrosis in glomerular mesangial cells.

The following Gpbar1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpbar1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12356	NM_177936.1 Latest version!	Rattus norvegicus G protein-coupled bile acid receptor 1 (Gpbar1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa12356	XM_006245235.3 Latest version!	Rattus norvegicus G protein-coupled bile acid receptor 1 (Gpbar1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpbar1 ( NM_177936.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_808797.1 Rattus norvegicus G protein-coupled bile acid receptor 1 (Gpbar1), mRNA.

Price & Availability↑

CloneID	ORa12356
Clone ID Related Accession (Same CDS sequence)	XM_006245235.3 , NM_177936.1
Accession Version	NM_177936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled bile acid receptor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB086172.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB086172.1 [ECO:0000332] ##Evidence-Data-END##

ATGATGTCAC ACAACACCAC TGAGCTGTCA GCCATTCCCA GAGGGGTTCA GGAGCTTTCC 
CTGGTCCTGG CAAGCCTCAT CGTCATCGCC AACCTGCTCC TGGCCCTAGG CATTGTCCTG 
GACCGCCACT TACGCAGCCC ACCTGCTGGC TGCTTCTTTC TAAGCCTACT ACTAGCTGGG 
CTACTCACAG GGTTGGCACT GCCCACGCTG CCTGGGCTAT GGAATAGGAG CCATCAGGGG 
TACTGGTCCT GCCTCCTTCT CCACTTGGCC CCCAACTTTT GTTTCCTCTC CCTGCTTGCC 
AATCTGCTGC TGGTGCATGG GGAACGCTAC ATGGCAGTGT TGCAGCCACT CCGGCCCCAT 
GGGAGTGTGC GGCTAGCCCT GTTCCTCACC TGGATCAGCT CCCTGCTCTT TGCCAGCCTG 
CCTGCTCTGG GCTGGAACCA CTGGAGTCCT GGTGCCAACT GCAGCTCCCA GGCTATCTTC 
CCAGCCCCCT ACCTTTACCT CGAAGTCTAT GGGCTCCTGC TGCCCGCTGT GGGGGCCACT 
GCCCTTCTCT CTGTCCGAGT GTTGGCCACT GCCCACCACC AGCTGCGGGA GATCCGCAGA 
CTGGAGCGGG CGGTGTGCCG TGATGCACCC TCAACCCTAG CGAGGGCTCT CACCTGGAGG 
CAGGCTAGGG CACAGGCAGG AGCCACACTG CTCTTTTTGC TGTGTTGGGG GCCCTATGTG 
GCCACATTGC TCCTGTCAGT CTTGGCCTAT GAGCGGCGGC CACCACTAGG GCCTGTAACT 
CTGTTATCTC TCATCTCATT GGGCAGTGCC AGTGCTGCAG TTGTGCCTGT GGCCATGGGT 
CTGGGTGATC AGCGCTACAC GGCCCCCTGG AGGACAGCTG CCCAAAGGTG GCTACAAGTG 
CTTCGAGGAA GACCCAAGAG GGCCAATCCA GGCCCCAGCA CTGCCTACCA CTCCAGTAGC 
CAATGCAGCA CTGACTTGGA CTTGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_808797.1
CDS	1..990
Translation	MMSHNTTELSAIPRGVQELSLVLASLIVIANLLLALGIVLDRHLRSPPAGCFFLSLLLAGLLTGLALPTLPGLWNRSHQGYWSCLLLHLAPNFCFLSLLANLLLVHGERYMAVLQPLRPHGSVRLALFLTWISSLLFASLPALGWNHWSPGANCSSQAIFPAPYLYLEVYGLLLPAVGATALLSVRVLATAHHQLREIRRLERAVCRDAPSTLARALTWRQARAQAGATLLFLLCWGPYVATLLLSVLAYERRPPLGPVTLLSLISLGSASAAVVPVAMGLGDQRYTAPWRTAAQRWLQVLRGRPKRANPGPSTAYHSSSQCSTDLDLN

Target ORF information:

RefSeq Version	NM_177936.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled bile acid receptor 1 (Gpbar1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177936.1

ORF Insert Sequence:

ATGATGTCAC ACAACACCAC TGAGCTGTCA GCCATTCCCA GAGGGGTTCA GGAGCTTTCC 
CTGGTCCTGG CAAGCCTCAT CGTCATCGCC AACCTGCTCC TGGCCCTAGG CATTGTCCTG 
GACCGCCACT TACGCAGCCC ACCTGCTGGC TGCTTCTTTC TAAGCCTACT ACTAGCTGGG 
CTACTCACAG GGTTGGCACT GCCCACGCTG CCTGGGCTAT GGAATAGGAG CCATCAGGGG 
TACTGGTCCT GCCTCCTTCT CCACTTGGCC CCCAACTTTT GTTTCCTCTC CCTGCTTGCC 
AATCTGCTGC TGGTGCATGG GGAACGCTAC ATGGCAGTGT TGCAGCCACT CCGGCCCCAT 
GGGAGTGTGC GGCTAGCCCT GTTCCTCACC TGGATCAGCT CCCTGCTCTT TGCCAGCCTG 
CCTGCTCTGG GCTGGAACCA CTGGAGTCCT GGTGCCAACT GCAGCTCCCA GGCTATCTTC 
CCAGCCCCCT ACCTTTACCT CGAAGTCTAT GGGCTCCTGC TGCCCGCTGT GGGGGCCACT 
GCCCTTCTCT CTGTCCGAGT GTTGGCCACT GCCCACCACC AGCTGCGGGA GATCCGCAGA 
CTGGAGCGGG CGGTGTGCCG TGATGCACCC TCAACCCTAG CGAGGGCTCT CACCTGGAGG 
CAGGCTAGGG CACAGGCAGG AGCCACACTG CTCTTTTTGC TGTGTTGGGG GCCCTATGTG 
GCCACATTGC TCCTGTCAGT CTTGGCCTAT GAGCGGCGGC CACCACTAGG GCCTGTAACT 
CTGTTATCTC TCATCTCATT GGGCAGTGCC AGTGCTGCAG TTGTGCCTGT GGCCATGGGT 
CTGGGTGATC AGCGCTACAC GGCCCCCTGG AGGACAGCTG CCCAAAGGTG GCTACAAGTG 
CTTCGAGGAA GACCCAAGAG GGCCAATCCA GGCCCCAGCA CTGCCTACCA CTCCAGTAGC 
CAATGCAGCA CTGACTTGGA CTTGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpbar1 ( XM_006245235.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245297.1 Rattus norvegicus G protein-coupled bile acid receptor 1 (Gpbar1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12356
Clone ID Related Accession (Same CDS sequence)	XM_006245235.3 , NM_177936.1
Accession Version	XM_006245235.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 990bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	G-protein coupled bile acid receptor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245235.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCAC ACAACACCAC TGAGCTGTCA GCCATTCCCA GAGGGGTTCA GGAGCTTTCC 
CTGGTCCTGG CAAGCCTCAT CGTCATCGCC AACCTGCTCC TGGCCCTAGG CATTGTCCTG 
GACCGCCACT TACGCAGCCC ACCTGCTGGC TGCTTCTTTC TAAGCCTACT ACTAGCTGGG 
CTACTCACAG GGTTGGCACT GCCCACGCTG CCTGGGCTAT GGAATAGGAG CCATCAGGGG 
TACTGGTCCT GCCTCCTTCT CCACTTGGCC CCCAACTTTT GTTTCCTCTC CCTGCTTGCC 
AATCTGCTGC TGGTGCATGG GGAACGCTAC ATGGCAGTGT TGCAGCCACT CCGGCCCCAT 
GGGAGTGTGC GGCTAGCCCT GTTCCTCACC TGGATCAGCT CCCTGCTCTT TGCCAGCCTG 
CCTGCTCTGG GCTGGAACCA CTGGAGTCCT GGTGCCAACT GCAGCTCCCA GGCTATCTTC 
CCAGCCCCCT ACCTTTACCT CGAAGTCTAT GGGCTCCTGC TGCCCGCTGT GGGGGCCACT 
GCCCTTCTCT CTGTCCGAGT GTTGGCCACT GCCCACCACC AGCTGCGGGA GATCCGCAGA 
CTGGAGCGGG CGGTGTGCCG TGATGCACCC TCAACCCTAG CGAGGGCTCT CACCTGGAGG 
CAGGCTAGGG CACAGGCAGG AGCCACACTG CTCTTTTTGC TGTGTTGGGG GCCCTATGTG 
GCCACATTGC TCCTGTCAGT CTTGGCCTAT GAGCGGCGGC CACCACTAGG GCCTGTAACT 
CTGTTATCTC TCATCTCATT GGGCAGTGCC AGTGCTGCAG TTGTGCCTGT GGCCATGGGT 
CTGGGTGATC AGCGCTACAC GGCCCCCTGG AGGACAGCTG CCCAAAGGTG GCTACAAGTG 
CTTCGAGGAA GACCCAAGAG GGCCAATCCA GGCCCCAGCA CTGCCTACCA CTCCAGTAGC 
CAATGCAGCA CTGACTTGGA CTTGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245297.1
CDS	2525..3514
Translation	MMSHNTTELSAIPRGVQELSLVLASLIVIANLLLALGIVLDRHLRSPPAGCFFLSLLLAGLLTGLALPTLPGLWNRSHQGYWSCLLLHLAPNFCFLSLLANLLLVHGERYMAVLQPLRPHGSVRLALFLTWISSLLFASLPALGWNHWSPGANCSSQAIFPAPYLYLEVYGLLLPAVGATALLSVRVLATAHHQLREIRRLERAVCRDAPSTLARALTWRQARAQAGATLLFLLCWGPYVATLLLSVLAYERRPPLGPVTLLSLISLGSASAAVVPVAMGLGDQRYTAPWRTAAQRWLQVLRGRPKRANPGPSTAYHSSSQCSTDLDLN

Target ORF information:

RefSeq Version	XM_006245235.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein-coupled bile acid receptor 1 (Gpbar1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245235.3

ORF Insert Sequence:

ATGATGTCAC ACAACACCAC TGAGCTGTCA GCCATTCCCA GAGGGGTTCA GGAGCTTTCC 
CTGGTCCTGG CAAGCCTCAT CGTCATCGCC AACCTGCTCC TGGCCCTAGG CATTGTCCTG 
GACCGCCACT TACGCAGCCC ACCTGCTGGC TGCTTCTTTC TAAGCCTACT ACTAGCTGGG 
CTACTCACAG GGTTGGCACT GCCCACGCTG CCTGGGCTAT GGAATAGGAG CCATCAGGGG 
TACTGGTCCT GCCTCCTTCT CCACTTGGCC CCCAACTTTT GTTTCCTCTC CCTGCTTGCC 
AATCTGCTGC TGGTGCATGG GGAACGCTAC ATGGCAGTGT TGCAGCCACT CCGGCCCCAT 
GGGAGTGTGC GGCTAGCCCT GTTCCTCACC TGGATCAGCT CCCTGCTCTT TGCCAGCCTG 
CCTGCTCTGG GCTGGAACCA CTGGAGTCCT GGTGCCAACT GCAGCTCCCA GGCTATCTTC 
CCAGCCCCCT ACCTTTACCT CGAAGTCTAT GGGCTCCTGC TGCCCGCTGT GGGGGCCACT 
GCCCTTCTCT CTGTCCGAGT GTTGGCCACT GCCCACCACC AGCTGCGGGA GATCCGCAGA 
CTGGAGCGGG CGGTGTGCCG TGATGCACCC TCAACCCTAG CGAGGGCTCT CACCTGGAGG 
CAGGCTAGGG CACAGGCAGG AGCCACACTG CTCTTTTTGC TGTGTTGGGG GCCCTATGTG 
GCCACATTGC TCCTGTCAGT CTTGGCCTAT GAGCGGCGGC CACCACTAGG GCCTGTAACT 
CTGTTATCTC TCATCTCATT GGGCAGTGCC AGTGCTGCAG TTGTGCCTGT GGCCATGGGT 
CTGGGTGATC AGCGCTACAC GGCCCCCTGG AGGACAGCTG CCCAAAGGTG GCTACAAGTG 
CTTCGAGGAA GACCCAAGAG GGCCAATCCA GGCCCCAGCA CTGCCTACCA CTCCAGTAGC 
CAATGCAGCA CTGACTTGGA CTTGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TGR5 contributes to hepatic cystogenesis in rodents with polycystic liver diseases through cyclic adenosine monophosphate/G?s signaling.
Hepatology (Baltimore, Md.)66(4)1197-1218(2017 10)
Masyuk TV,Masyuk AI,Lorenzo Pisarello M,Howard BN,Huang BQ,Lee PY,Fung X,Sergienko E,Ardecky RJ,Chung TDY,Pinkerton AB,LaRusso NF

The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance.
BMC endocrine disorders17(1)60(2017 Sep)
Yan X,Li P,Tang Z,Feng B

TUDCA: An Agonist of the Bile Acid Receptor GPBAR1/TGR5 With Anti-Inflammatory Effects in Microglial Cells.
Journal of cellular physiology232(8)2231-2245(2017 Aug)
Yanguas-Cas?s N,Barreda-Manso MA,Nieto-Sampedro M,Romero-Ram?rez L

Bile acid receptor TGR5 overexpression is associated with decreased intestinal mucosal injury and epithelial cell proliferation in obstructive jaundice.
Translational research : the journal of laboratory and clinical medicine18288-102(2017 Apr)
Ji CG,Xie XL,Yin J,Qi W,Chen L,Bai Y,Wang N,Zhao DQ,Jiang XY,Jiang HQ

TGR5 suppresses high glucose-induced upregulation of fibronectin and transforming growth factor-?1 in rat glomerular mesangial cells by inhibiting RhoA/ROCK signaling.
Endocrine54(3)657-670(2016 Dec)
Xiong F,Li X,Yang Z,Wang Y,Gong W,Huang J,Chen C,Liu P,Huang H

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Gpbar1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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