Gldn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gldn
Entrez Gene ID	315675
Full Name	gliomedin
Synonyms	Colm,Crgl2
General protein information	Preferred Names gliomedin Names gliomedin collomin olfactomedin-related protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24
Summary	cytoplasm protein; mouse homolog is highly expressed in hepatocellular carcinomas [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_181382.2	NP_852047.1	gliomedin

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gldn	NP_796324.1
Gallus gallus (chicken)	GLDN	XP_425097.4
Homo sapiens (human)	GLDN	NP_861454.2
Pan troglodytes (chimpanzee)	GLDN	XP_001169660.1
Canis lupus familiaris (dog)	GLDN	XP_535480.3
Bos taurus (cattle)	GLDN	XP_002690971.1
Rattus norvegicus (Norway rat)	Gldn	NP_852047.1
Xenopus tropicalis (tropical clawed frog)	gldn	XP_002936383.2
Danio rerio (zebrafish)	gldn	NP_001073634.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in GLDN, Encoding Gliomedin, a Critical Component of the Nodes of Ranvier, Are Responsible for Lethal Arthrogryposis.
Maluenda J, Manso C, Quevarec L, Vivanti A, Marguet F, Gonzales M, Guimiot F, Petit F, Toutain A, Whalen S, Grigorescu R, Coeslier AD, Gut M, Gut I, Laquerri?re A, Devaux J, Melki J
American journal of human genetics99(4)928-933(2016 Oct)

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the extracellular olfactomedin domain of gliomedin.
Han H, Kursula P
Acta crystallographica. Section F, Structural biology communications70(Pt 11)1536-9(2014 Nov)

Antibodies to gliomedin cause peripheral demyelinating neuropathy and the dismantling of the nodes of Ranvier.
Devaux JJ
The American journal of pathology181(4)1402-13(2012 Oct)

A glial signal consisting of gliomedin and NrCAM clusters axonal Na+ channels during the formation of nodes of Ranvier.
Feinberg K, Eshed-Eisenbach Y, Frechter S, Amor V, Salomon D, Sabanay H, Dupree JL, Grumet M, Brophy PJ, Shrager P, Peles E
Neuron65(4)490-502(2010 Feb)

Cleavage and oligomerization of gliomedin, a transmembrane collagen required for node of ranvier formation.
Maertens B, Hopkins D, Franzke CW, Keene DR, Bruckner-Tuderman L, Greenspan DS, Koch M
The Journal of biological chemistry282(14)10647-59(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The crystallization of the rat GLDN olfactomedin domain was expressed in an insect-cell system.
Title: Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the extracellular olfactomedin domain of gliomedin.

Primary immune reaction against gliomedin, a peripheral nervous system adhesion molecule, can be responsible for the initiation or progression of the demyelinating form of Guillain-Barre syndrome.
Title: Antibodies to gliomedin cause peripheral demyelinating neuropathy and the dismantling of the nodes of Ranvier.

The following Gldn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gldn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04319	NM_181382.2 Latest version!	Rattus norvegicus gliomedin (Gldn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$321.30 $459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gldn ( NM_181382.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_852047.1 Rattus norvegicus gliomedin (Gldn), mRNA.

Price & Availability↑

CloneID	ORa04319
Clone ID Related Accession (Same CDS sequence)	NM_181382.2
Accession Version	NM_181382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	gliomedin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY266116.1. On Jun 3, 2003 this sequence version replaced NM_181382.1. ##Evidence-Data-START## Transcript exon combination :: AY266116.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACCCGAG CCGCAGAGCG AGGCCAAGGG GCTACAGGCT GGGGACTGCG AGGCGCCCTG 
ATGGCCGTGG CGCTGCTGTC AGTGCTGAAC GCCGTGGGCA CCGTGTTCGT GCTGTACCAG 
TGGCGCGAGC TGAGCGCGGC GCTGCGGGCA CTGGAGGCGC AACACGGCCA GGAGCAGCGC 
GAGGACAGCG CCCTACGCGC CTTTCTAGCT GAATTAAGTC GTGCGCCAGC CCGAGTCCCC 
GAACCACCCC AGGACCCCAT GAGTGCAGCG CGCAATAAGC GCAGCCACGG CGGCGAGCCT 
GCGTCACACA TCCGCGCGGA GAGCCAGGAC ATGATGATGA TGATGACCTA CAGCATGGTG 
CCGATCCGGG TGATGATAGA CCTGTGCAAC AGCACCCAGG GCATCTGCCT TACAGGACCA 
CCGGGCCCAC CAGGACCTCC AGGAGCTGGT GGGTTACCAG GCCACAATGG ATCAGATGGA 
CAGCCTGGTC TCCAGGGCCC AAAAGGAGAA AAAGGAGCAG TTGGGAAGAG AGGAAAAATG 
GGGTTACCCG GAGCCACAGG AAATCCAGGG GAAAAGGGAG AGAAGGGAGA TGCTGGTGAA 
CTGGGCCTAC CTGGAAATGA GGGACCACCA GGACAGAAAG GAGACAAAGG AGACAAAGGA 
GATGTGTCCA ATGACGTGCT TTTGACAGGT GCCAAAGGTG ACCAAGGGCC CCCTGGCCCA 
CCTGGACCCC CAGGGCCTCC AGGCCCTCCT GGAAGCAGAA GAGCCAAAGG CCCTCGGCAG 
CCAAATTCGT TCACCAACCA GTGTCCAGGG GAGACGTGTG TCATACCCAA TGATGATACC 
TTGGTGGGGA GAGCTGATGA GAAAGTCAAT GAGCGCCATT CTCCACAAAC AGAACCCATG 
ATCACGTCCA TTGGTAACCC GGCCCAAGTC CTCAAGGTGA AAGAGACTTT TGGGACCTGG 
CTAAGAGAGT CTGCTAACAG GAGTGACGAC CGCATTTGGG TGACTGAACA TTTTTCAGGC 
ATCATGGTGA AGGAGTTTGA AGACCTGCCC GCCCTCCTGA ATAGCAGCTT CACCCTCCTC 
CACCTCCCAC ATTACTTCCA TGGCTGCGGG CACGCTGTTT ACAACAACTC TCTCTACTAC 
CACAAAGGAG GCTCCAACAC CATAGTGAGA TTTGAATTTG GGAAAGAGAC ACCTCAAACT 
CTGAAGCTTG AAGATGCTTT GTATTTTGAT CGAAAATACC TCTTTGCGAA TTCCAAGACT 
TACTTCAACA TAGCAGTGGA TGAGAAGGGC CTCTGGATTA TCTACGCCTC GAGTGTGGAT 
GGCTCAAGCA TCCTTGTGGC ACAGCTGGAC GAGAGGACAT TCTCTGTGCT GCGGCACATC 
AATACCACAT ACCCCAAGTC CAAGGCTGGC AATGCCTTCA TAGCTCAAGG GATCCTCTAT 
GTCACGGACA CCAAAGATAC AAGGGTCACG TTTGCCTTTG ATTTGTTACG AGGGAAGCAG 
ATCAATGCAA ACTTCGGTCT CAGAATGTCA CAGTCTGTTC TTGCCATGTT GTCGTACAAT 
ATGAGAGACC AGCATTTGTA CTCGTGGGAA GACGGCCACC TGATGCTCTA TCCTGTGCAC 
TTTTCGTCAA CAGCACCCAG CCAGCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852047.1
CDS	189..1838
Translation	MTRAAERGQGATGWGLRGALMAVALLSVLNAVGTVFVLYQWRELSAALRALEAQHGQEQREDSALRAFLAELSRAPARVPEPPQDPMSAARNKRSHGGEPASHIRAESQDMMMMMTYSMVPIRVMIDLCNSTQGICLTGPPGPPGPPGAGGLPGHNGSDGQPGLQGPKGEKGAVGKRGKMGLPGATGNPGEKGEKGDAGELGLPGNEGPPGQKGDKGDKGDVSNDVLLTGAKGDQGPPGPPGPPGPPGPPGSRRAKGPRQPNSFTNQCPGETCVIPNDDTLVGRADEKVNERHSPQTEPMITSIGNPAQVLKVKETFGTWLRESANRSDDRIWVTEHFSGIMVKEFEDLPALLNSSFTLLHLPHYFHGCGHAVYNNSLYYHKGGSNTIVRFEFGKETPQTLKLEDALYFDRKYLFANSKTYFNIAVDEKGLWIIYASSVDGSSILVAQLDERTFSVLRHINTTYPKSKAGNAFIAQGILYVTDTKDTRVTFAFDLLRGKQINANFGLRMSQSVLAMLSYNMRDQHLYSWEDGHLMLYPVHFSSTAPSQR

Target ORF information:

RefSeq Version	NM_181382.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus gliomedin (Gldn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181382.2

ORF Insert Sequence:

ATGACCCGAG CCGCAGAGCG AGGCCAAGGG GCTACAGGCT GGGGACTGCG AGGCGCCCTG 
ATGGCCGTGG CGCTGCTGTC AGTGCTGAAC GCCGTGGGCA CCGTGTTCGT GCTGTACCAG 
TGGCGCGAGC TGAGCGCGGC GCTGCGGGCA CTGGAGGCGC AACACGGCCA GGAGCAGCGC 
GAGGACAGCG CCCTACGCGC CTTTCTAGCT GAATTAAGTC GTGCGCCAGC CCGAGTCCCC 
GAACCACCCC AGGACCCCAT GAGTGCAGCG CGCAATAAGC GCAGCCACGG CGGCGAGCCT 
GCGTCACACA TCCGCGCGGA GAGCCAGGAC ATGATGATGA TGATGACCTA CAGCATGGTG 
CCGATCCGGG TGATGATAGA CCTGTGCAAC AGCACCCAGG GCATCTGCCT TACAGGACCA 
CCGGGCCCAC CAGGACCTCC AGGAGCTGGT GGGTTACCAG GCCACAATGG ATCAGATGGA 
CAGCCTGGTC TCCAGGGCCC AAAAGGAGAA AAAGGAGCAG TTGGGAAGAG AGGAAAAATG 
GGGTTACCCG GAGCCACAGG AAATCCAGGG GAAAAGGGAG AGAAGGGAGA TGCTGGTGAA 
CTGGGCCTAC CTGGAAATGA GGGACCACCA GGACAGAAAG GAGACAAAGG AGACAAAGGA 
GATGTGTCCA ATGACGTGCT TTTGACAGGT GCCAAAGGTG ACCAAGGGCC CCCTGGCCCA 
CCTGGACCCC CAGGGCCTCC AGGCCCTCCT GGAAGCAGAA GAGCCAAAGG CCCTCGGCAG 
CCAAATTCGT TCACCAACCA GTGTCCAGGG GAGACGTGTG TCATACCCAA TGATGATACC 
TTGGTGGGGA GAGCTGATGA GAAAGTCAAT GAGCGCCATT CTCCACAAAC AGAACCCATG 
ATCACGTCCA TTGGTAACCC GGCCCAAGTC CTCAAGGTGA AAGAGACTTT TGGGACCTGG 
CTAAGAGAGT CTGCTAACAG GAGTGACGAC CGCATTTGGG TGACTGAACA TTTTTCAGGC 
ATCATGGTGA AGGAGTTTGA AGACCTGCCC GCCCTCCTGA ATAGCAGCTT CACCCTCCTC 
CACCTCCCAC ATTACTTCCA TGGCTGCGGG CACGCTGTTT ACAACAACTC TCTCTACTAC 
CACAAAGGAG GCTCCAACAC CATAGTGAGA TTTGAATTTG GGAAAGAGAC ACCTCAAACT 
CTGAAGCTTG AAGATGCTTT GTATTTTGAT CGAAAATACC TCTTTGCGAA TTCCAAGACT 
TACTTCAACA TAGCAGTGGA TGAGAAGGGC CTCTGGATTA TCTACGCCTC GAGTGTGGAT 
GGCTCAAGCA TCCTTGTGGC ACAGCTGGAC GAGAGGACAT TCTCTGTGCT GCGGCACATC 
AATACCACAT ACCCCAAGTC CAAGGCTGGC AATGCCTTCA TAGCTCAAGG GATCCTCTAT 
GTCACGGACA CCAAAGATAC AAGGGTCACG TTTGCCTTTG ATTTGTTACG AGGGAAGCAG 
ATCAATGCAA ACTTCGGTCT CAGAATGTCA CAGTCTGTTC TTGCCATGTT GTCGTACAAT 
ATGAGAGACC AGCATTTGTA CTCGTGGGAA GACGGCCACC TGATGCTCTA TCCTGTGCAC 
TTTTCGTCAA CAGCACCCAG CCAGCGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in GLDN, Encoding Gliomedin, a Critical Component of the Nodes of Ranvier, Are Responsible for Lethal Arthrogryposis.
American journal of human genetics99(4)928-933(2016 Oct)
Maluenda J,Manso C,Quevarec L,Vivanti A,Marguet F,Gonzales M,Guimiot F,Petit F,Toutain A,Whalen S,Grigorescu R,Coeslier AD,Gut M,Gut I,Laquerri?re A,Devaux J,Melki J

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of the extracellular olfactomedin domain of gliomedin.
Acta crystallographica. Section F, Structural biology communications70(Pt 11)1536-9(2014 Nov)
Han H,Kursula P

Antibodies to gliomedin cause peripheral demyelinating neuropathy and the dismantling of the nodes of Ranvier.
The American journal of pathology181(4)1402-13(2012 Oct)
Devaux JJ

A glial signal consisting of gliomedin and NrCAM clusters axonal Na+ channels during the formation of nodes of Ranvier.
Neuron65(4)490-502(2010 Feb)
Feinberg K,Eshed-Eisenbach Y,Frechter S,Amor V,Salomon D,Sabanay H,Dupree JL,Grumet M,Brophy PJ,Shrager P,Peles E

Cleavage and oligomerization of gliomedin, a transmembrane collagen required for node of ranvier formation.
The Journal of biological chemistry282(14)10647-59(2007 Apr)
Maertens B,Hopkins D,Franzke CW,Keene DR,Bruckner-Tuderman L,Greenspan DS,Koch M