Amelx cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Amelx
Entrez Gene ID	29160
Full Name	amelogenin, X-linked
Synonyms	Amel,LRAP
General protein information	Preferred Names amelogenin, X-linked Names amelogenin, X isoform amelogenin X chromosome leucine-rich amelogenin peptide
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq13
Summary	may play a role in mineralization of tooth enamel matrix [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271077.1	NP_001258006.1	amelogenin, X isoform isoform 6 precursor
NM_001271078.1	NP_001258007.1	amelogenin, X isoform isoform 7 precursor
NM_001271074.1	NP_001258003.1	amelogenin, X isoform isoform 1 precursor
NM_001271073.1	NP_001258002.1	amelogenin, X isoform isoform 2 precursor
NM_001271076.1	NP_001258005.1	amelogenin, X isoform isoform 4 precursor
NM_019154.1	NP_062027.1	amelogenin, X isoform isoform 5 precursor
NM_001271075.1	NP_001258004.1	amelogenin, X isoform isoform 3 precursor
NM_019154.2	NP_062027.1	amelogenin, X isoform isoform 5 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	AMELX	NP_872621.1
Canis lupus familiaris (dog)	AMELX	XP_003435493.1
Rattus norvegicus (Norway rat)	Amelx	NP_062027.1
Bos taurus (cattle)	AMELX	NP_001014984.1
	AMELX	NP_001091983.1

Gene Ontology
Bibliography

Related articles in PubMed

A solution NMR investigation into the impaired self-assembly properties of two murine amelogenins containing the point mutations T21?I or P41?T.
Buchko GW, Lin G, Tarasevich BJ, Shaw WJ
Archives of biochemistry and biophysics537(2)217-24(2013 Sep)

Leucine-rich amelogenin peptides regulate mineralization in vitro.
Le Norcy E, Kwak SY, Wiedemann-Bidlack FB, Beniash E, Yamakoshi Y, Simmer JP, Margolis HC
Journal of dental research90(9)1091-7(2011 Sep)

A solution NMR investigation into the murine amelogenin splice-variant LRAP (Leucine-Rich Amelogenin Protein).
Buchko GW, Tarasevich BJ, Roberts J, Snead ML, Shaw WJ
Biochimica et biophysica acta1804(9)1768-74(2010 Sep)

Amelogenin-collagen interactions regulate calcium phosphate mineralization in vitro.
Deshpande AS, Fang PA, Simmer JP, Margolis HC, Beniash E
The Journal of biological chemistry285(25)19277-87(2010 Jun)

GTPases RhoA and Rac1 are important for amelogenin and DSPP expression during differentiation of ameloblasts and odontoblasts.
Biz MT, Marques MR, Crema VO, Moriscot AS, dos Santos MF
Cell and tissue research340(3)459-70(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

interactions between collagen and amelogenin might play an important role in the formation of the DEB providing structural continuity between dentin and enamel
Title: Amelogenin-collagen interactions regulate calcium phosphate mineralization in vitro.

The aim of this study was to verify the expression, distribution and role of Rho GTPases in ameloblasts and odontoblasts during tooth development in correlation with actin and tubulin arrangements and amelogenin and dentin sialophosphoprotein expression.
Title: GTPases RhoA and Rac1 are important for amelogenin and DSPP expression during differentiation of ameloblasts and odontoblasts.

This study identified a suppressor element in the promoter region and enhancer elements in intron 1 of amelogenin in HAT-7 and HeLA cells.
Title: Identification of a suppressor element in the amelogenin promoter.

subpopulation of granules containing only ameloblastin could be responsible for augmenting its local concentration along secretory surfaces against which hydroxyapatite crystals actively elongate
Title: Ameloblastin and amelogenin share a common secretory pathway and are co-secreted during enamel formation.

during differentiation, dental epithelial cells utilize a unique mechanism for increasing the production of amelogenin, the reuptake of secreted amelogenin
Title: Reuptake of extracellular amelogenin by dental epithelial cells results in increased levels of amelogenin mRNA through enhanced mRNA stabilization.

The following Amelx gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Amelx cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10160	NM_001271075.1 Latest version!	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa10163	NM_001271076.1 Latest version!	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa10173	NM_001271078.1 Latest version!	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa03871	NM_019154.2 Latest version!	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
ORa10152	NM_001271073.1 Latest version!	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
ORa10156	NM_001271074.1 Latest version!	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
ORa10167	NM_001271077.1 Latest version!	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
ORa03871	NM_019154.1	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Amelx ( NM_001271075.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258004.1 Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa10160
Clone ID Related Accession (Same CDS sequence)	NM_001271075.1
Accession Version	NM_001271075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Rattus norvegicus(Norway rat)
Product	amelogenin, X isoform isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U60562.1 and U60564.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site at the 5' end of an exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U60564.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGCCC CTGTCCCCCA TTCTTCCTGA GCTGCCTCTG 
GAAGCTTGGC CAGCGACAGA CAAGACCAAG CGGGAAGAAG TGGCATTTTC TCCTATGAAG 
TGGTACCAGG GCACGGCAAG GCATCCGCTT AACATGGAAA CCACAACAGA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258004.1
CDS	67..363
Translation	MGTWILFACLLGAAFAMPLPPHPGSPGYINLSYEVLTPLKWYQSMIRQPPLSPILPELPLEAWPATDKTKREEVAFSPMKWYQGTARHPLNMETTTEK

Target ORF information:

RefSeq Version	NM_001271075.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271075.1

ORF Insert Sequence:

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGCCC CTGTCCCCCA TTCTTCCTGA GCTGCCTCTG 
GAAGCTTGGC CAGCGACAGA CAAGACCAAG CGGGAAGAAG TGGCATTTTC TCCTATGAAG 
TGGTACCAGG GCACGGCAAG GCATCCGCTT AACATGGAAA CCACAACAGA AAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amelx ( NM_001271076.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258005.1 Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 4, mRNA.

Price & Availability↑

CloneID	ORa10163
Clone ID Related Accession (Same CDS sequence)	NM_001271076.1
Accession Version	NM_001271076.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Rattus norvegicus(Norway rat)
Product	amelogenin, X isoform isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U60562.1 and U60563.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGGCA TTTTCTCCTA TGAAGTGGTA CCAGGGCACG 
GCAAGGCATC CGCTTAACAT GGAAACCACA ACAGAAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258005.1
CDS	67..288
Translation	MGTWILFACLLGAAFAMPLPPHPGSPGYINLSYEVLTPLKWYQSMIRQPAFSPMKWYQGTARHPLNMETTTEK

Target ORF information:

RefSeq Version	NM_001271076.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271076.1

ORF Insert Sequence:

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGGCA TTTTCTCCTA TGAAGTGGTA CCAGGGCACG 
GCAAGGCATC CGCTTAACAT GGAAACCACA ACAGAAAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amelx ( NM_001271078.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258007.1 Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 7, mRNA.

Price & Availability↑

CloneID	ORa10173
Clone ID Related Accession (Same CDS sequence)	NM_001271078.1
Accession Version	NM_001271078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Rattus norvegicus(Norway rat)
Product	amelogenin, X isoform isoform 7 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U60562.1, U60564.1 and U67130.1. Transcript Variant: This variant (7) uses an alternate in-frame splice site at the 5' end of an exon and differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (7) lacks an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U07054.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGCCC CTGTCCCCCA TTCTTCCTGA GCTGCCTCTG 
GAAGCTTGGC CAGCGACAGA CAAGACCAAG CGGGAAGAAG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258007.1
CDS	67..294
Translation	MGTWILFACLLGAAFAMPLPPHPGSPGYINLSYEVLTPLKWYQSMIRQPPLSPILPELPLEAWPATDKTKREEVD

Target ORF information:

RefSeq Version	NM_001271078.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271078.1

ORF Insert Sequence:

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGCCC CTGTCCCCCA TTCTTCCTGA GCTGCCTCTG 
GAAGCTTGGC CAGCGACAGA CAAGACCAAG CGGGAAGAAG TGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amelx ( NM_019154.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062027.1 Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 5, mRNA.

Price & Availability↑

CloneID	ORa03871
Clone ID Related Accession (Same CDS sequence)	NM_019154.1 , NM_019154.2
Accession Version	NM_019154.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	amelogenin, X isoform isoform 5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U67130.1. Transcript Variant: This variant (5) contains an alternate in-frame exon and differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (5) contains an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U67130.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052297, SAMN02642716 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGAAGTCACA TTCTCAGGCT 
ATCAATACTG ACAGGACTGC ATTAGTGCTT ACCCCCTTGA AGTGGTACCA GAGCATGATA 
AGGCAGCCGT ATCCTTCCTA TGGTTACGAA CCCATGGGTG GATGGCTGCA CCACCAAATC 
ATCCCTGTGC TGTCTCAACA GCATCCCCCG AGTCACACCC TTCAGCCTCA TCACCACCTC 
CCAGTGGTGC CAGCTCAACA GCCCGTGGCC CCCCAGCAAC CAATGATGCC AGTTCCTGGC 
CACCACTCCA TGACTCCAAC CCAACACCAT CAGCCAAACA TCCCTCCATC CGCCCAGCAG 
CCCTTCCAGC AGCCCTTCCA GCCCCAGGCC ATTCCACCCC AGTCTCATCA GCCCATGCAG 
CCCCAGTCAC CTCTGCATCC CATGCAGCCC CTGGCACCAC AGCCACCTCT GCCTCCACTG 
TTCTCCATGC AGCCCCTGTC CCCCATTCTT CCTGAGCTGC CTCTGGAAGC TTGGCCAGCG 
ACAGACAAGA CCAAGCGGGA AGAAGTGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062027.1
CDS	16..648
Translation	MGTWILFACLLGAAFAMPLPPHPGSPGYINLSYEKSHSQAINTDRTALVLTPLKWYQSMIRQPYPSYGYEPMGGWLHHQIIPVLSQQHPPSHTLQPHHHLPVVPAQQPVAPQQPMMPVPGHHSMTPTQHHQPNIPPSAQQPFQQPFQPQAIPPQSHQPMQPQSPLHPMQPLAPQPPLPPLFSMQPLSPILPELPLEAWPATDKTKREEVD

Target ORF information:

RefSeq Version	NM_019154.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019154.1

ORF Insert Sequence:

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGAAGTCACA TTCTCAGGCT 
ATCAATACTG ACAGGACTGC ATTAGTGCTT ACCCCCTTGA AGTGGTACCA GAGCATGATA 
AGGCAGCCGT ATCCTTCCTA TGGTTACGAA CCCATGGGTG GATGGCTGCA CCACCAAATC 
ATCCCTGTGC TGTCTCAACA GCATCCCCCG AGTCACACCC TTCAGCCTCA TCACCACCTC 
CCAGTGGTGC CAGCTCAACA GCCCGTGGCC CCCCAGCAAC CAATGATGCC AGTTCCTGGC 
CACCACTCCA TGACTCCAAC CCAACACCAT CAGCCAAACA TCCCTCCATC CGCCCAGCAG 
CCCTTCCAGC AGCCCTTCCA GCCCCAGGCC ATTCCACCCC AGTCTCATCA GCCCATGCAG 
CCCCAGTCAC CTCTGCATCC CATGCAGCCC CTGGCACCAC AGCCACCTCT GCCTCCACTG 
TTCTCCATGC AGCCCCTGTC CCCCATTCTT CCTGAGCTGC CTCTGGAAGC TTGGCCAGCG 
ACAGACAAGA CCAAGCGGGA AGAAGTGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amelx ( NM_019154.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062027.1 Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 5, mRNA.

Price & Availability↑

CloneID	ORa03871
Clone ID Related Accession (Same CDS sequence)	NM_019154.1 , NM_019154.2
Accession Version	NM_019154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Rattus norvegicus(Norway rat)
Product	amelogenin, X isoform isoform 5 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U60562.1 and U67130.1. On Jan 18, 2018 this sequence version replaced NM_019154.1. Transcript Variant: This variant (5) contains an alternate in-frame exon and differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (5) contains an alternate internal segment and has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U67130.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGAAGTCACA TTCTCAGGCT 
ATCAATACTG ACAGGACTGC ATTAGTGCTT ACCCCCTTGA AGTGGTACCA GAGCATGATA 
AGGCAGCCGT ATCCTTCCTA TGGTTACGAA CCCATGGGTG GATGGCTGCA CCACCAAATC 
ATCCCTGTGC TGTCTCAACA GCATCCCCCG AGTCACACCC TTCAGCCTCA TCACCACCTC 
CCAGTGGTGC CAGCTCAACA GCCCGTGGCC CCCCAGCAAC CAATGATGCC AGTTCCTGGC 
CACCACTCCA TGACTCCAAC CCAACACCAT CAGCCAAACA TCCCTCCATC CGCCCAGCAG 
CCCTTCCAGC AGCCCTTCCA GCCCCAGGCC ATTCCACCCC AGTCTCATCA GCCCATGCAG 
CCCCAGTCAC CTCTGCATCC CATGCAGCCC CTGGCACCAC AGCCACCTCT GCCTCCACTG 
TTCTCCATGC AGCCCCTGTC CCCCATTCTT CCTGAGCTGC CTCTGGAAGC TTGGCCAGCG 
ACAGACAAGA CCAAGCGGGA AGAAGTGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062027.1
CDS	67..699
Translation	MGTWILFACLLGAAFAMPLPPHPGSPGYINLSYEKSHSQAINTDRTALVLTPLKWYQSMIRQPYPSYGYEPMGGWLHHQIIPVLSQQHPPSHTLQPHHHLPVVPAQQPVAPQQPMMPVPGHHSMTPTQHHQPNIPPSAQQPFQQPFQPQAIPPQSHQPMQPQSPLHPMQPLAPQPPLPPLFSMQPLSPILPELPLEAWPATDKTKREEVD

Target ORF information:

RefSeq Version	NM_019154.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019154.2

ORF Insert Sequence:

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGAAGTCACA TTCTCAGGCT 
ATCAATACTG ACAGGACTGC ATTAGTGCTT ACCCCCTTGA AGTGGTACCA GAGCATGATA 
AGGCAGCCGT ATCCTTCCTA TGGTTACGAA CCCATGGGTG GATGGCTGCA CCACCAAATC 
ATCCCTGTGC TGTCTCAACA GCATCCCCCG AGTCACACCC TTCAGCCTCA TCACCACCTC 
CCAGTGGTGC CAGCTCAACA GCCCGTGGCC CCCCAGCAAC CAATGATGCC AGTTCCTGGC 
CACCACTCCA TGACTCCAAC CCAACACCAT CAGCCAAACA TCCCTCCATC CGCCCAGCAG 
CCCTTCCAGC AGCCCTTCCA GCCCCAGGCC ATTCCACCCC AGTCTCATCA GCCCATGCAG 
CCCCAGTCAC CTCTGCATCC CATGCAGCCC CTGGCACCAC AGCCACCTCT GCCTCCACTG 
TTCTCCATGC AGCCCCTGTC CCCCATTCTT CCTGAGCTGC CTCTGGAAGC TTGGCCAGCG 
ACAGACAAGA CCAAGCGGGA AGAAGTGGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amelx ( NM_001271073.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258002.1 Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa10152
Clone ID Related Accession (Same CDS sequence)	NM_001271073.1
Accession Version	NM_001271073.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Rattus norvegicus(Norway rat)
Product	amelogenin, X isoform isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U60562.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U60562.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGCAT CCCCCGAGTC ACACCCTTCA GCCTCATCAC 
CACCTCCCAG TGGTGCCAGC TCAACAGCCC GTGGCCCCCC AGCAACCAAT GATGCCAGTT 
CCTGGCCACC ACTCCATGAC TCCAACCCAA CACCATCAGC CAAACATCCC TCCATCCGCC 
CAGCAGCCCT TCCAGCAGCC CTTCCAGCCC CAGGCCATTC CACCCCAGTC TCATCAGCCC 
ATGCAGCCCC AGTCACCTCT GCATCCCATG CAGCCCCTGG CACCACAGCC ACCTCTGCCT 
CCACTGTTCT CCATGCAGCC CCTGTCCCCC ATTCTTCCTG AGCTGCCTCT GGAAGCTTGG 
CCAGCGACAG ACAAGACCAA GCGGGAAGAA GTGGCATTTT CTCCTATGAA GTGGTACCAG 
GGCACGGCAA GGCATCCGCT TAACATGGAA ACCACAACAG AAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258002.1
CDS	67..654
Translation	MGTWILFACLLGAAFAMPLPPHPGSPGYINLSYEVLTPLKWYQSMIRQPHPPSHTLQPHHHLPVVPAQQPVAPQQPMMPVPGHHSMTPTQHHQPNIPPSAQQPFQQPFQPQAIPPQSHQPMQPQSPLHPMQPLAPQPPLPPLFSMQPLSPILPELPLEAWPATDKTKREEVAFSPMKWYQGTARHPLNMETTTEK

Target ORF information:

RefSeq Version	NM_001271073.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271073.1

ORF Insert Sequence:

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGCAT CCCCCGAGTC ACACCCTTCA GCCTCATCAC 
CACCTCCCAG TGGTGCCAGC TCAACAGCCC GTGGCCCCCC AGCAACCAAT GATGCCAGTT 
CCTGGCCACC ACTCCATGAC TCCAACCCAA CACCATCAGC CAAACATCCC TCCATCCGCC 
CAGCAGCCCT TCCAGCAGCC CTTCCAGCCC CAGGCCATTC CACCCCAGTC TCATCAGCCC 
ATGCAGCCCC AGTCACCTCT GCATCCCATG CAGCCCCTGG CACCACAGCC ACCTCTGCCT 
CCACTGTTCT CCATGCAGCC CCTGTCCCCC ATTCTTCCTG AGCTGCCTCT GGAAGCTTGG 
CCAGCGACAG ACAAGACCAA GCGGGAAGAA GTGGCATTTT CTCCTATGAA GTGGTACCAG 
GGCACGGCAA GGCATCCGCT TAACATGGAA ACCACAACAG AAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amelx ( NM_001271074.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258003.1 Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 1, mRNA.

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CloneID	ORa10156
Clone ID Related Accession (Same CDS sequence)	NM_001271074.1
Accession Version	NM_001271074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Rattus norvegicus(Norway rat)
Product	amelogenin, X isoform isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07037645.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U51195.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGTAT CCTTCCTATG GTTACGAACC CATGGGTGGA 
TGGCTGCACC ACCAAATCAT CCCTGTGCTG TCTCAACAGC ATCCCCCGAG TCACACCCTT 
CAGCCTCATC ACCACCTCCC AGTGGTGCCA GCTCAACAGC CCGTGGCCCC CCAGCAACCA 
ATGATGCCAG TTCCTGGCCA CCACTCCATG ACTCCAACCC AACACCATCA GCCAAACATC 
CCTCCATCCG CCCAGCAGCC CTTCCAGCAG CCCTTCCAGC CCCAGGCCAT TCCACCCCAG 
TCTCATCAGC CCATGCAGCC CCAGTCACCT CTGCATCCCA TGCAGCCCCT GGCACCACAG 
CCACCTCTGC CTCCACTGTT CTCCATGCAG CCCCTGTCCC CCATTCTTCC TGAGCTGCCT 
CTGGAAGCTT GGCCAGCGAC AGACAAGACC AAGCGGGAAG AAGTGGCATT TTCTCCTATG 
AAGTGGTACC AGGGCACGGC AAGGCATCCG CTTAACATGG AAACCACAAC AGAAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258003.1
CDS	67..726
Translation	MGTWILFACLLGAAFAMPLPPHPGSPGYINLSYEVLTPLKWYQSMIRQPYPSYGYEPMGGWLHHQIIPVLSQQHPPSHTLQPHHHLPVVPAQQPVAPQQPMMPVPGHHSMTPTQHHQPNIPPSAQQPFQQPFQPQAIPPQSHQPMQPQSPLHPMQPLAPQPPLPPLFSMQPLSPILPELPLEAWPATDKTKREEVAFSPMKWYQGTARHPLNMETTTEK

Target ORF information:

RefSeq Version	NM_001271074.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271074.1

ORF Insert Sequence:

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGTAT CCTTCCTATG GTTACGAACC CATGGGTGGA 
TGGCTGCACC ACCAAATCAT CCCTGTGCTG TCTCAACAGC ATCCCCCGAG TCACACCCTT 
CAGCCTCATC ACCACCTCCC AGTGGTGCCA GCTCAACAGC CCGTGGCCCC CCAGCAACCA 
ATGATGCCAG TTCCTGGCCA CCACTCCATG ACTCCAACCC AACACCATCA GCCAAACATC 
CCTCCATCCG CCCAGCAGCC CTTCCAGCAG CCCTTCCAGC CCCAGGCCAT TCCACCCCAG 
TCTCATCAGC CCATGCAGCC CCAGTCACCT CTGCATCCCA TGCAGCCCCT GGCACCACAG 
CCACCTCTGC CTCCACTGTT CTCCATGCAG CCCCTGTCCC CCATTCTTCC TGAGCTGCCT 
CTGGAAGCTT GGCCAGCGAC AGACAAGACC AAGCGGGAAG AAGTGGCATT TTCTCCTATG 
AAGTGGTACC AGGGCACGGC AAGGCATCCG CTTAACATGG AAACCACAAC AGAAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Amelx ( NM_001271077.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258006.1 Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 6, mRNA.

Price & Availability↑

CloneID	ORa10167
Clone ID Related Accession (Same CDS sequence)	NM_001271077.1
Accession Version	NM_001271077.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 591bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Rattus norvegicus(Norway rat)
Product	amelogenin, X isoform isoform 6 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U60562.1 and U67130.1. Transcript Variant: This variant (6) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (6) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: U01245.2 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGTAT CCTTCCTATG GTTACGAACC CATGGGTGGA 
TGGCTGCACC ACCAAATCAT CCCTGTGCTG TCTCAACAGC ATCCCCCGAG TCACACCCTT 
CAGCCTCATC ACCACCTCCC AGTGGTGCCA GCTCAACAGC CCGTGGCCCC CCAGCAACCA 
ATGATGCCAG TTCCTGGCCA CCACTCCATG ACTCCAACCC AACACCATCA GCCAAACATC 
CCTCCATCCG CCCAGCAGCC CTTCCAGCAG CCCTTCCAGC CCCAGGCCAT TCCACCCCAG 
TCTCATCAGC CCATGCAGCC CCAGTCACCT CTGCATCCCA TGCAGCCCCT GGCACCACAG 
CCACCTCTGC CTCCACTGTT CTCCATGCAG CCCCTGTCCC CCATTCTTCC TGAGCTGCCT 
CTGGAAGCTT GGCCAGCGAC AGACAAGACC AAGCGGGAAG AAGTGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258006.1
CDS	67..657
Translation	MGTWILFACLLGAAFAMPLPPHPGSPGYINLSYEVLTPLKWYQSMIRQPYPSYGYEPMGGWLHHQIIPVLSQQHPPSHTLQPHHHLPVVPAQQPVAPQQPMMPVPGHHSMTPTQHHQPNIPPSAQQPFQQPFQPQAIPPQSHQPMQPQSPLHPMQPLAPQPPLPPLFSMQPLSPILPELPLEAWPATDKTKREEVD

Target ORF information:

RefSeq Version	NM_001271077.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus amelogenin, X-linked (Amelx), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271077.1

ORF Insert Sequence:

ATGGGGACCT GGATCTTGTT TGCCTGCCTC CTGGGAGCAG CTTTTGCTAT GCCCCTACCA 
CCTCATCCTG GGAGCCCTGG TTATATCAAC TTAAGCTATG AGGTGCTTAC CCCCTTGAAG 
TGGTACCAGA GCATGATAAG GCAGCCGTAT CCTTCCTATG GTTACGAACC CATGGGTGGA 
TGGCTGCACC ACCAAATCAT CCCTGTGCTG TCTCAACAGC ATCCCCCGAG TCACACCCTT 
CAGCCTCATC ACCACCTCCC AGTGGTGCCA GCTCAACAGC CCGTGGCCCC CCAGCAACCA 
ATGATGCCAG TTCCTGGCCA CCACTCCATG ACTCCAACCC AACACCATCA GCCAAACATC 
CCTCCATCCG CCCAGCAGCC CTTCCAGCAG CCCTTCCAGC CCCAGGCCAT TCCACCCCAG 
TCTCATCAGC CCATGCAGCC CCAGTCACCT CTGCATCCCA TGCAGCCCCT GGCACCACAG 
CCACCTCTGC CTCCACTGTT CTCCATGCAG CCCCTGTCCC CCATTCTTCC TGAGCTGCCT 
CTGGAAGCTT GGCCAGCGAC AGACAAGACC AAGCGGGAAG AAGTGGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A solution NMR investigation into the impaired self-assembly properties of two murine amelogenins containing the point mutations T21?I or P41?T.
Archives of biochemistry and biophysics537(2)217-24(2013 Sep)
Buchko GW,Lin G,Tarasevich BJ,Shaw WJ

Leucine-rich amelogenin peptides regulate mineralization in vitro.
Journal of dental research90(9)1091-7(2011 Sep)
Le Norcy E,Kwak SY,Wiedemann-Bidlack FB,Beniash E,Yamakoshi Y,Simmer JP,Margolis HC

A solution NMR investigation into the murine amelogenin splice-variant LRAP (Leucine-Rich Amelogenin Protein).
Biochimica et biophysica acta1804(9)1768-74(2010 Sep)
Buchko GW,Tarasevich BJ,Roberts J,Snead ML,Shaw WJ

Amelogenin-collagen interactions regulate calcium phosphate mineralization in vitro.
The Journal of biological chemistry285(25)19277-87(2010 Jun)
Deshpande AS,Fang PA,Simmer JP,Margolis HC,Beniash E

GTPases RhoA and Rac1 are important for amelogenin and DSPP expression during differentiation of ameloblasts and odontoblasts.
Cell and tissue research340(3)459-70(2010 Jun)
Biz MT,Marques MR,Crema VO,Moriscot AS,dos Santos MF

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Amelx cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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