Ankrd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ankrd1
Entrez Gene ID	27064
Full Name	ankyrin repeat domain 1
Synonyms	Alrp,Carp,Crap,MARP
General protein information	Preferred Names ankyrin repeat domain 1 Names ankyrin repeat domain-containing protein 1 ankyrin repeat domain 1 (cardiac muscle) ankyrin-like repeat protein cardiac adriamycin-responsive protein cardiac ankyrin repeat protein cardiac responsive adriamycin protein muscle ankyrin repeat protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q53
Summary	acts as a negative regulator of cardiac-specific gene expression [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008760363.2	XP_008758585.2	ankyrin repeat domain-containing protein 1 isoform X1
NM_013220.1	NP_037352.1	ankyrin repeat domain-containing protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ANKRD1	XP_001144141.1
	ANKRD1	XP_001087855.1
Bos taurus (cattle)	ANKRD1	NP_001029550.1
Rattus norvegicus (Norway rat)	Ankrd1	NP_037352.1
Danio rerio (zebrafish)	ankrd1a	XP_001920092.1
Xenopus tropicalis (tropical clawed frog)	ankrd1	NP_001025607.1
Homo sapiens (human)	ANKRD1	NP_055206.2
Mus musculus (house mouse)	Ankrd1	NP_038496.2
Gallus gallus (chicken)	ANKRD1	NP_989736.1
Canis lupus familiaris (dog)	ANKRD1	XP_005637588.1

Gene Ontology
Bibliography

Related articles in PubMed

MLP and CARP are linked to chronic PKC? signalling in dilated cardiomyopathy.
Lange S, Gehmlich K, Lun AS, Blondelle J, Hooper C, Dalton ND, Alvarez EA, Zhang X, Bang ML, Abassi YA, Dos Remedios CG, Peterson KL, Chen J, Ehler E
Nature communications712120(2016 Jun)

Cytosolic CARP promotes angiotensin II- or pressure overload-induced cardiomyocyte hypertrophy through calcineurin accumulation.
Chen C, Shen L, Cao S, Li X, Xuan W, Zhang J, Huang X, Bin J, Xu D, Li G, Kitakaze M, Liao Y
PloS one9(8)e104040(2014)

Ankrd1 is a transcriptional repressor for the androgen receptor that is downregulated by testosterone.
Wu Y, Ruggiero CL, Bauman WA, Cardozo C
Biochemical and biophysical research communications437(3)355-60(2013 Aug)

26S proteasome regulation of Ankrd1/CARP in adult rat ventricular myocytes and human microvascular endothelial cells.
Samaras SE, Chen B, Koch SR, Sawyer DB, Lim CC, Davidson JM
Biochemical and biophysical research communications425(4)830-5(2012 Sep)

Disruption of a GATA4/Ankrd1 signaling axis in cardiomyocytes leads to sarcomere disarray: implications for anthracycline cardiomyopathy.
Chen B, Zhong L, Roush SF, Pentassuglia L, Peng X, Samaras S, Davidson JM, Sawyer DB, Lim CC
PloS one7(4)e35743(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of Ankrd1 exacerbates pathological cardiac remodeling through the enhancement of cytosolic translocation of CARP and up-regulation of calcineurin.
Title: Cytosolic CARP promotes angiotensin II- or pressure overload-induced cardiomyocyte hypertrophy through calcineurin accumulation.

Ankrd1 binds to and directly regulates the transcriptional activity of the human androgen receptor in cultured rat myoblasts, whereas Ankrd2 does not.
Title: Ankrd1 is a transcriptional repressor for the androgen receptor that is downregulated by testosterone.

the 26S proteasome is the dominant regulator of Ankrd1/CARP degradation, and that Ankrd1/CARP half-life is significantly longer in cardiomyocytes (h) than endothelial cells (min).
Title: 26S proteasome regulation of Ankrd1/CARP in adult rat ventricular myocytes and human microvascular endothelial cells.

These data demonstrate co-dependent roles for GATA4 and CARP in regulating sarcomere gene expression and maintaining sarcomeric organization in cardiomyocytes in culture.
Title: Disruption of a GATA4/Ankrd1 signaling axis in cardiomyocytes leads to sarcomere disarray: implications for anthracycline cardiomyopathy.

Data show that CARP specific expressed in mouse heart and mainly localized in the nucleus in rat cardiac primary cells.
Title: [Polyclonal antibody preparation and expression pattern analysis of CARP].

The following Ankrd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ankrd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02370	NM_013220.1 Latest version!	Rattus norvegicus ankyrin repeat domain 1 (Ankrd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa56417	XM_008760363.2 Latest version!	Rattus norvegicus ankyrin repeat domain 1 (Ankrd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ankrd1 ( NM_013220.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037352.1 Rattus norvegicus ankyrin repeat domain 1 (Ankrd1), mRNA.

Price & Availability↑

CloneID	ORa02370
Clone ID Related Accession (Same CDS sequence)	NM_013220.1
Accession Version	NM_013220.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin repeat domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U50736.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U50736.1, BC072699.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052297, SAMEA2689595 [ECO:0000350] ##Evidence-Data-END##

ATGATGGTTT TTCGAGTAGA GGAGCTGGTA ACGGGCAAAA AGAACAGCAA TGGGTCCTCA 
GGGGAGTTCC TTCCTGGCGA GTTCAGAAAT GGAGAGTATG AAGCTGCTGT TGCCTTGGAG 
AAACAAGAGG ACTTGAAGAC ACTTCCAGCC AACAGTGTGA ACCTGGGGGA AGAACAACGG 
AAAAGTGAGA AAGTTCGAGA GGCAGAGCTC AAAAAGAAAA AACTAGAACA AAGATCAAAG 
CTTGAAAACT TAGAAGACCT TGAAATAATT GTTCAACTGA AGAAAAGAAA AAAATACAAG 
AAAACTAAAG TTCCAGTTGT GAAGGAACCG GAGCCTGAAA TTATTACTGA ACCTGTGGAT 
GTGCCAAGGT TTCTGAAAGC TGCACTGGAG AACAAACTGC CAGTTGTAGA GAAATTCCTG 
TCAGACAAGA ACAGCCCCGA CGTCTGCGAT GAGTATAAAC GGACCGCTCT CCATAGAGCA 
TGCTTAGAAG GACACTTGGC GATCGTGGAG AAGTTAATGG AGGCTGGAGC CCAGATTGAA 
TTCCGAGATA TGCTGGAATC CACAGCCATC CACTGGGCAT GTCGTGGAGG AAACCTGGAT 
GTCCTGAAAC TGTTGCTAAA CAAAGGTGCC AAAATCAGTG CCCGAGACAA GCTGCTCAGC 
ACAGCGCTGC ACGTGGCGGT GAGGACCGGT CACTACGAGT GTGCTGAGCA CCTCATCGCC 
TGCGAGGCGG ATCTCAATGC CAAGGACAGA GAAGGAGACA CCCCTCTGCA TGATGCGGTG 
AGGTTGAATC GCTACAAGAT GATCCGGCTC TTGATGACCT TCGGTGCGGA CCTCAATGTC 
AAGAACTGTG CTGGGAAGAT CCCTATGGAT CTGGTGTTGC ACTGGCAGAA TGGAACCAAA 
GCGATATTCG ACAGCCTCAA GGAGAATGCC TACAAAAACT CGCGCATAGC TACGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037352.1
CDS	61..1020
Translation	MMVFRVEELVTGKKNSNGSSGEFLPGEFRNGEYEAAVALEKQEDLKTLPANSVNLGEEQRKSEKVREAELKKKKLEQRSKLENLEDLEIIVQLKKRKKYKKTKVPVVKEPEPEIITEPVDVPRFLKAALENKLPVVEKFLSDKNSPDVCDEYKRTALHRACLEGHLAIVEKLMEAGAQIEFRDMLESTAIHWACRGGNLDVLKLLLNKGAKISARDKLLSTALHVAVRTGHYECAEHLIACEADLNAKDREGDTPLHDAVRLNRYKMIRLLMTFGADLNVKNCAGKIPMDLVLHWQNGTKAIFDSLKENAYKNSRIATF

Target ORF information:

RefSeq Version	NM_013220.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat domain 1 (Ankrd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013220.1

ORF Insert Sequence:

ATGATGGTTT TTCGAGTAGA GGAGCTGGTA ACGGGCAAAA AGAACAGCAA TGGGTCCTCA 
GGGGAGTTCC TTCCTGGCGA GTTCAGAAAT GGAGAGTATG AAGCTGCTGT TGCCTTGGAG 
AAACAAGAGG ACTTGAAGAC ACTTCCAGCC AACAGTGTGA ACCTGGGGGA AGAACAACGG 
AAAAGTGAGA AAGTTCGAGA GGCAGAGCTC AAAAAGAAAA AACTAGAACA AAGATCAAAG 
CTTGAAAACT TAGAAGACCT TGAAATAATT GTTCAACTGA AGAAAAGAAA AAAATACAAG 
AAAACTAAAG TTCCAGTTGT GAAGGAACCG GAGCCTGAAA TTATTACTGA ACCTGTGGAT 
GTGCCAAGGT TTCTGAAAGC TGCACTGGAG AACAAACTGC CAGTTGTAGA GAAATTCCTG 
TCAGACAAGA ACAGCCCCGA CGTCTGCGAT GAGTATAAAC GGACCGCTCT CCATAGAGCA 
TGCTTAGAAG GACACTTGGC GATCGTGGAG AAGTTAATGG AGGCTGGAGC CCAGATTGAA 
TTCCGAGATA TGCTGGAATC CACAGCCATC CACTGGGCAT GTCGTGGAGG AAACCTGGAT 
GTCCTGAAAC TGTTGCTAAA CAAAGGTGCC AAAATCAGTG CCCGAGACAA GCTGCTCAGC 
ACAGCGCTGC ACGTGGCGGT GAGGACCGGT CACTACGAGT GTGCTGAGCA CCTCATCGCC 
TGCGAGGCGG ATCTCAATGC CAAGGACAGA GAAGGAGACA CCCCTCTGCA TGATGCGGTG 
AGGTTGAATC GCTACAAGAT GATCCGGCTC TTGATGACCT TCGGTGCGGA CCTCAATGTC 
AAGAACTGTG CTGGGAAGAT CCCTATGGAT CTGGTGTTGC ACTGGCAGAA TGGAACCAAA 
GCGATATTCG ACAGCCTCAA GGAGAATGCC TACAAAAACT CGCGCATAGC TACGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd1 ( XM_008760363.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758585.2 Rattus norvegicus ankyrin repeat domain 1 (Ankrd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56417
Clone ID Related Accession (Same CDS sequence)	XM_008760363.2
Accession Version	XM_008760363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin repeat domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAT CCCCAAACAT GGCTTGGAAG GGACGAAGGT GCTGCAAAAA GCCGAGAGAT 
AAAACTGGTC AGGTGAGAAC TATGCCATCA AGCCACAAGC GACATGCTCA GCCTGGAGTA 
ACGGGCAAAA AGAACAGCAA TGGGTCCTCA GGGGAGTTCC TTCCTGGCGA GTTCAGAAAT 
GGAGAGTATG AAGCTGCTGT TGCCTTGGAG AAACAAGAGG ACTTGAAGAC ACTTCCAGCC 
AACAGTGTGA ACCTGGGGGA AGAACAACGG AAAAGTGAGA AAGTTCGAGA GGCAGAGCTC 
AAAAAGAAAA AACTAGAACA AAGATCAAAG CTTGAAAACT TAGAAGACCT TGAAATAATT 
GTTCAACTGA AGAAAAGAAA AAAATACAAG AAAACTAAAG TTCCAGTTGT GAAGGAACCG 
GAGCCTGAAA TTATTACTGA ACCTGTGGAT GTGCCAAGGT TTCTGAAAGC TGCACTGGAG 
AACAAACTGC CAGTTGTAGA GAAATTCCTG TCAGACAAGA ACAGCCCCGA CGTCTGCGAT 
GAGTATAAAC GGACCGCTCT CCATAGAGCA TGCTTAGAAG GACACTTGGC GATCGTGGAG 
AAGTTAATGG AGGCTGGAGC CCAGATTGAA TTCCGAGATA TGCTGGAATC CACAGCCATC 
CACTGGGCAT GTCGTGGAGG AAACCTGGAT GTCCTGAAAC TGTTGCTAAA CAAAGGTGCC 
AAAATCAGTG CCCGAGACAA GCTGCTCAGC ACAGCGCTGC ACGTGGCGGT GAGGACCGGT 
CACTACGAGT GTGCTGAGCA CCTCATCGCC TGCGAGGCGG ATCTCAATGC CAAGGACAGA 
GAAGGAGACA CCCCTCTGCA TGATGCGGTG AGGTTGAATC GCTACAAGAT GATCCGGCTC 
TTGATGACCT TCGGTGCGGA CCTCAATGTC AAGAACTGTG CTGGGAAGAC CCCTATGGAT 
CTGGTGTTGC ACTGGCAGAA TGGAACCAAA GCGATATTCG ACAGCCTCAA GGAGAATGCC 
TACAAAAACT CGCGCATAGC TACGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758585.2
CDS	21..1070
Translation	MAASPNMAWKGRRCCKKPRDKTGQVRTMPSSHKRHAQPGVTGKKNSNGSSGEFLPGEFRNGEYEAAVALEKQEDLKTLPANSVNLGEEQRKSEKVREAELKKKKLEQRSKLENLEDLEIIVQLKKRKKYKKTKVPVVKEPEPEIITEPVDVPRFLKAALENKLPVVEKFLSDKNSPDVCDEYKRTALHRACLEGHLAIVEKLMEAGAQIEFRDMLESTAIHWACRGGNLDVLKLLLNKGAKISARDKLLSTALHVAVRTGHYECAEHLIACEADLNAKDREGDTPLHDAVRLNRYKMIRLLMTFGADLNVKNCAGKTPMDLVLHWQNGTKAIFDSLKENAYKNSRIATF

Target ORF information:

RefSeq Version	XM_008760363.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat domain 1 (Ankrd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760363.2

ORF Insert Sequence:

ATGGCAGCAT CCCCAAACAT GGCTTGGAAG GGACGAAGGT GCTGCAAAAA GCCGAGAGAT 
AAAACTGGTC AGGTGAGAAC TATGCCATCA AGCCACAAGC GACATGCTCA GCCTGGAGTA 
ACGGGCAAAA AGAACAGCAA TGGGTCCTCA GGGGAGTTCC TTCCTGGCGA GTTCAGAAAT 
GGAGAGTATG AAGCTGCTGT TGCCTTGGAG AAACAAGAGG ACTTGAAGAC ACTTCCAGCC 
AACAGTGTGA ACCTGGGGGA AGAACAACGG AAAAGTGAGA AAGTTCGAGA GGCAGAGCTC 
AAAAAGAAAA AACTAGAACA AAGATCAAAG CTTGAAAACT TAGAAGACCT TGAAATAATT 
GTTCAACTGA AGAAAAGAAA AAAATACAAG AAAACTAAAG TTCCAGTTGT GAAGGAACCG 
GAGCCTGAAA TTATTACTGA ACCTGTGGAT GTGCCAAGGT TTCTGAAAGC TGCACTGGAG 
AACAAACTGC CAGTTGTAGA GAAATTCCTG TCAGACAAGA ACAGCCCCGA CGTCTGCGAT 
GAGTATAAAC GGACCGCTCT CCATAGAGCA TGCTTAGAAG GACACTTGGC GATCGTGGAG 
AAGTTAATGG AGGCTGGAGC CCAGATTGAA TTCCGAGATA TGCTGGAATC CACAGCCATC 
CACTGGGCAT GTCGTGGAGG AAACCTGGAT GTCCTGAAAC TGTTGCTAAA CAAAGGTGCC 
AAAATCAGTG CCCGAGACAA GCTGCTCAGC ACAGCGCTGC ACGTGGCGGT GAGGACCGGT 
CACTACGAGT GTGCTGAGCA CCTCATCGCC TGCGAGGCGG ATCTCAATGC CAAGGACAGA 
GAAGGAGACA CCCCTCTGCA TGATGCGGTG AGGTTGAATC GCTACAAGAT GATCCGGCTC 
TTGATGACCT TCGGTGCGGA CCTCAATGTC AAGAACTGTG CTGGGAAGAC CCCTATGGAT 
CTGGTGTTGC ACTGGCAGAA TGGAACCAAA GCGATATTCG ACAGCCTCAA GGAGAATGCC 
TACAAAAACT CGCGCATAGC TACGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MLP and CARP are linked to chronic PKC? signalling in dilated cardiomyopathy.
Nature communications712120(2016 Jun)
Lange S,Gehmlich K,Lun AS,Blondelle J,Hooper C,Dalton ND,Alvarez EA,Zhang X,Bang ML,Abassi YA,Dos Remedios CG,Peterson KL,Chen J,Ehler E

Cytosolic CARP promotes angiotensin II- or pressure overload-induced cardiomyocyte hypertrophy through calcineurin accumulation.
PloS one9(8)e104040(2014)
Chen C,Shen L,Cao S,Li X,Xuan W,Zhang J,Huang X,Bin J,Xu D,Li G,Kitakaze M,Liao Y

Ankrd1 is a transcriptional repressor for the androgen receptor that is downregulated by testosterone.
Biochemical and biophysical research communications437(3)355-60(2013 Aug)
Wu Y,Ruggiero CL,Bauman WA,Cardozo C

26S proteasome regulation of Ankrd1/CARP in adult rat ventricular myocytes and human microvascular endothelial cells.
Biochemical and biophysical research communications425(4)830-5(2012 Sep)
Samaras SE,Chen B,Koch SR,Sawyer DB,Lim CC,Davidson JM

Disruption of a GATA4/Ankrd1 signaling axis in cardiomyocytes leads to sarcomere disarray: implications for anthracycline cardiomyopathy.
PloS one7(4)e35743(2012)
Chen B,Zhong L,Roush SF,Pentassuglia L,Peng X,Samaras S,Davidson JM,Sawyer DB,Lim CC

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Ankrd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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