Has2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Has2
Entrez Gene ID	25694
Full Name	hyaluronan synthase 2
General protein information	Preferred Names hyaluronan synthase 2 Names hyaluronan synthase 2 HA synthase 2 hyaluronate synthase 2 hyaluronic acid synthase 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q33
Summary	membrane protein that synthesizes hyaluronan, a high molecular weight polysaccharide that is a component of the extracellular matrix; may be important for epidermal proliferation, thickness and differentiation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013153.1	NP_037285.1	hyaluronan synthase 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HAS2	XP_539153.1
Xenopus tropicalis (tropical clawed frog)	has2	NP_001120557.1
Homo sapiens (human)	HAS2	NP_005319.1
Bos taurus (cattle)	HAS2	NP_776504.1
Danio rerio (zebrafish)	has2	NP_705936.1
Pan troglodytes (chimpanzee)	HAS2	XP_528222.2
Mus musculus (house mouse)	Has2	NP_032242.3
	HAS2	XP_001098841.1
Rattus norvegicus (Norway rat)	Has2	NP_037285.1
Gallus gallus (chicken)	HAS2	NP_990137.1
Magnaporthe oryzae (rice blast fungus)	MGG_15353	XP_003716009.1

Gene Ontology
Bibliography

Related articles in PubMed

CRISPR/Cas9 knockout of HAS2 in rat chondrosarcoma chondrocytes demonstrates the requirement of hyaluronan for aggrecan retention.
Huang Y, Askew EB, Knudson CB, Knudson W
Matrix biology : journal of the International Society for Matrix Biology5674-94(2016 Dec)

Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3.
Bart G, Vico NO, Hassinen A, Pujol FM, Deen AJ, Ruusala A, Tammi RH, Squire A, Heldin P, Kellokumpu S, Tammi MI
The Journal of biological chemistry290(18)11479-90(2015 May)

Homeostasis of hyaluronic acid in normal and scarred vocal folds.
Tateya I, Tateya T, Watanuki M, Bless DM
Journal of voice : official journal of the Voice Foundation29(2)133-9(2015 Mar)

Involvement of hyaluronan synthesis in ovarian follicle growth in rats.
Takahashi N, Tarumi W, Ishizuka B
Reproduction (Cambridge, England)147(2)189-97(2014 Feb)

Tissue distribution and subcellular localization of hyaluronan synthase isoenzymes.
T?rr?nen K, Nikunen K, K?rn? R, Tammi M, Tammi R, Rilla K
Histochemistry and cell biology141(1)17-31(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression patterns of Has and Hyal genes differed between normal, acute-scarred, and chronic-scarred vocal folds, indicating the distinct roles of each enzyme in maintaining HA.
Title: Homeostasis of hyaluronic acid in normal and scarred vocal folds.

Data indicate that p38 and Ca(2+)/calmodulin-dependent protein kinase II pathways were found to be involved in the UVB-induced up-regulation of Has2 and Has3 expression.
Title: Low dose ultraviolet B irradiation increases hyaluronan synthesis in epidermal keratinocytes via sequential induction of hyaluronan synthases Has1-3 mediated by p38 and Ca2+/calmodulin-dependent protein kinase II (CaMKII) signaling.

HAS-1 expression was significantly decreased on rat vocal fold postinjury days 3, 7, 14, 28, and 56; HAS-2 expression was significantly decreased on postinjury days 28 and 56; HAS-3 expression was significantly decreased on postinjury day 56.
Title: Extracellular matrix gene expression during wound healing of the injured rat vocal fold.

Results provide evidence of HGF treatment effects on procollagen type III and HAS-2 gene expression pathways.
Title: Effect of hepatocyte growth factor on gene expression of extracellular matrix during wound healing of the injured rat vocal fold.

Increased expression of Has2 and MMP-1 genes from BSCs may have a positive potential in the treatment of vocal fold scarring.
Title: Expression of extracellular matrix proteins in the vocal folds and bone marrow derived stromal cells of rats.

The following Has2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Has2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02139	NM_013153.1 Latest version!	Rattus norvegicus hyaluronan synthase 2 (Has2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$328.30 $469.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Has2 ( NM_013153.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037285.1 Rattus norvegicus hyaluronan synthase 2 (Has2), mRNA.

Price & Availability↑

CloneID	ORa02139
Clone ID Related Accession (Same CDS sequence)	NM_013153.1
Accession Version	NM_013153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	hyaluronan synthase 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF008201.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF008201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689602, SAMN00632258 [ECO:0000348] ##Evidence-Data-END##

ATGCATTGTG AGAGGTTTCT ATGTGTCCTG AGAATAATCG GAACCACACT GTTTGGAGTG 
TCTCTCCTCC TCGGAATCAC AGCTGCTTAT ATTGTTGGCT ACCAGTTTAT CCAAACAGAT 
AATTACTATT TCTCCTTTGG GCTGTATGGT GCCTTTTTAG CCTCACACCT CATCATCCAA 
AGCCTCTTTG CCTTTTTGGA ACACCGGAAA ATGAAAAAGT CCCTTGAAAC CCCCATTAAA 
TTGAACAAAA CTGTAGCTCT CTGCATTGCT GCGTATCAAG AAGACCCTGA CTACTTACGG 
AAATGTTTGC AATCTGTGAA AAGGCTGACC TACCCTGGGA TCAAAGTCGT GATGGTCATA 
GATGGAAATT CAGATGACGA CCTTTACATG ATGGACATCT TCAGTGAAGT CATGGGCAGG 
GACAAATCAG TCACTTACAT CTGGAAGAAC AACTTCCATG AAAGGGGACC TGGTGAGACA 
GAAGAGTCCC ATAAAGAAAG TTCGCAACAT GTAACCCAAT TGGTCTTGTC TAACAAGAGT 
ATTTGCATCA TGCAAAAATG GGGTGGAAAG AGAGAAGTCA TGTACACCGC CTTCAGAGCA 
CTGGGCAGAA GCGTGGATTA TGTACAGGTG TGTGACTCAG ACACCATGCT TGACCCTGCC 
TCGTCTGTGG AGATGGTGAA GGTCTTAGAA GAAGACCCGA TGGTTGGAGG TGTTGGGGGA 
GATGTTCAGA TTTTAAACAA GTATGATTCT TGGATCTCCT TCCTCAGCAG TGTGAGATAC 
TGGATGGCTT TTAATATAGA AAGGGCCTGC CAGTCTTATT TTGGCTGTGT CCAGTGCATA 
AGCGGTCCTC TGGGAATGTA CAGAAACTCC TTGTTGCATG AGTTTGTGGA AGACTGGTAC 
AATCAGGAAT TCATGGGTAA CCAATGCAGT TTCGGTGATG ATAGGCACCT TACCAACAGG 
GTACTGAGTC TGGGCTATGC AACTAAATAC ACGGCTCGGT CCAAGTGCCT TACTGAAACA 
CCCATAGAAT ATCTGAGATG GCTGAACCAG CAGACCCGTT GGAGCAAGTC CTACTTCCGA 
GAGTGGCTAT ACAATGCCAT GTGGTTTCAC AAGCATCACT TGTGGATGAC CTACGAAGCT 
GTTATCACTG GATTCTTTCC TTTCTTTCTC ATTGCCACAG TCATCCAGCT CTTCTACAGG 
GGTAAAATCT GGAACATCCT CCTCTTCCTG TTAACTGTCC AGCTAGTGGG TCTCATCAAG 
TCGTCTTTCG CCAGCTGCCT TAGAGGAAAT ATCGTCATGG TCTTCATGTC TCTCTACTCA 
GTGTTGTACA TGTCAAGTCT ACTTCCTGCC AAGATGTTTG CAATTGCAAC CATAAACAAA 
GCTGGGTGGG GCACATCTGG AAGGAAAACC ATTGTCGTTA ATTTCATAGG ACTTATTCCA 
GTGTCCGTGT GGTTTACAAT CCTTCTAGGT GGTGTGATTT TCACCATTTA TAAGGAATCT 
AAAAAGCCAT TTTCCGAATC CAAACAGACT GTTCTCATTG TGGGAACCTT GATCTATGCG 
TGCTACTGGG TCGTGCTTTT GACTCTGTAT GTGGTTCTCA TCAATAAGTG TGGCAGGCGG 
AAGAAGGGAC AACAGTACGA CATGGTGCTT GATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037285.1
CDS	507..2165
Translation	MHCERFLCVLRIIGTTLFGVSLLLGITAAYIVGYQFIQTDNYYFSFGLYGAFLASHLIIQSLFAFLEHRKMKKSLETPIKLNKTVALCIAAYQEDPDYLRKCLQSVKRLTYPGIKVVMVIDGNSDDDLYMMDIFSEVMGRDKSVTYIWKNNFHERGPGETEESHKESSQHVTQLVLSNKSICIMQKWGGKREVMYTAFRALGRSVDYVQVCDSDTMLDPASSVEMVKVLEEDPMVGGVGGDVQILNKYDSWISFLSSVRYWMAFNIERACQSYFGCVQCISGPLGMYRNSLLHEFVEDWYNQEFMGNQCSFGDDRHLTNRVLSLGYATKYTARSKCLTETPIEYLRWLNQQTRWSKSYFREWLYNAMWFHKHHLWMTYEAVITGFFPFFLIATVIQLFYRGKIWNILLFLLTVQLVGLIKSSFASCLRGNIVMVFMSLYSVLYMSSLLPAKMFAIATINKAGWGTSGRKTIVVNFIGLIPVSVWFTILLGGVIFTIYKESKKPFSESKQTVLIVGTLIYACYWVVLLTLYVVLINKCGRRKKGQQYDMVLDV

Target ORF information:

RefSeq Version	NM_013153.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hyaluronan synthase 2 (Has2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013153.1

ORF Insert Sequence:

ATGCATTGTG AGAGGTTTCT ATGTGTCCTG AGAATAATCG GAACCACACT GTTTGGAGTG 
TCTCTCCTCC TCGGAATCAC AGCTGCTTAT ATTGTTGGCT ACCAGTTTAT CCAAACAGAT 
AATTACTATT TCTCCTTTGG GCTGTATGGT GCCTTTTTAG CCTCACACCT CATCATCCAA 
AGCCTCTTTG CCTTTTTGGA ACACCGGAAA ATGAAAAAGT CCCTTGAAAC CCCCATTAAA 
TTGAACAAAA CTGTAGCTCT CTGCATTGCT GCGTATCAAG AAGACCCTGA CTACTTACGG 
AAATGTTTGC AATCTGTGAA AAGGCTGACC TACCCTGGGA TCAAAGTCGT GATGGTCATA 
GATGGAAATT CAGATGACGA CCTTTACATG ATGGACATCT TCAGTGAAGT CATGGGCAGG 
GACAAATCAG TCACTTACAT CTGGAAGAAC AACTTCCATG AAAGGGGACC TGGTGAGACA 
GAAGAGTCCC ATAAAGAAAG TTCGCAACAT GTAACCCAAT TGGTCTTGTC TAACAAGAGT 
ATTTGCATCA TGCAAAAATG GGGTGGAAAG AGAGAAGTCA TGTACACCGC CTTCAGAGCA 
CTGGGCAGAA GCGTGGATTA TGTACAGGTG TGTGACTCAG ACACCATGCT TGACCCTGCC 
TCGTCTGTGG AGATGGTGAA GGTCTTAGAA GAAGACCCGA TGGTTGGAGG TGTTGGGGGA 
GATGTTCAGA TTTTAAACAA GTATGATTCT TGGATCTCCT TCCTCAGCAG TGTGAGATAC 
TGGATGGCTT TTAATATAGA AAGGGCCTGC CAGTCTTATT TTGGCTGTGT CCAGTGCATA 
AGCGGTCCTC TGGGAATGTA CAGAAACTCC TTGTTGCATG AGTTTGTGGA AGACTGGTAC 
AATCAGGAAT TCATGGGTAA CCAATGCAGT TTCGGTGATG ATAGGCACCT TACCAACAGG 
GTACTGAGTC TGGGCTATGC AACTAAATAC ACGGCTCGGT CCAAGTGCCT TACTGAAACA 
CCCATAGAAT ATCTGAGATG GCTGAACCAG CAGACCCGTT GGAGCAAGTC CTACTTCCGA 
GAGTGGCTAT ACAATGCCAT GTGGTTTCAC AAGCATCACT TGTGGATGAC CTACGAAGCT 
GTTATCACTG GATTCTTTCC TTTCTTTCTC ATTGCCACAG TCATCCAGCT CTTCTACAGG 
GGTAAAATCT GGAACATCCT CCTCTTCCTG TTAACTGTCC AGCTAGTGGG TCTCATCAAG 
TCGTCTTTCG CCAGCTGCCT TAGAGGAAAT ATCGTCATGG TCTTCATGTC TCTCTACTCA 
GTGTTGTACA TGTCAAGTCT ACTTCCTGCC AAGATGTTTG CAATTGCAAC CATAAACAAA 
GCTGGGTGGG GCACATCTGG AAGGAAAACC ATTGTCGTTA ATTTCATAGG ACTTATTCCA 
GTGTCCGTGT GGTTTACAAT CCTTCTAGGT GGTGTGATTT TCACCATTTA TAAGGAATCT 
AAAAAGCCAT TTTCCGAATC CAAACAGACT GTTCTCATTG TGGGAACCTT GATCTATGCG 
TGCTACTGGG TCGTGCTTTT GACTCTGTAT GTGGTTCTCA TCAATAAGTG TGGCAGGCGG 
AAGAAGGGAC AACAGTACGA CATGGTGCTT GATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CRISPR/Cas9 knockout of HAS2 in rat chondrosarcoma chondrocytes demonstrates the requirement of hyaluronan for aggrecan retention.
Matrix biology : journal of the International Society for Matrix Biology5674-94(2016 Dec)
Huang Y,Askew EB,Knudson CB,Knudson W

Fluorescence resonance energy transfer (FRET) and proximity ligation assays reveal functionally relevant homo- and heteromeric complexes among hyaluronan synthases HAS1, HAS2, and HAS3.
The Journal of biological chemistry290(18)11479-90(2015 May)
Bart G,Vico NO,Hassinen A,Pujol FM,Deen AJ,Ruusala A,Tammi RH,Squire A,Heldin P,Kellokumpu S,Tammi MI

Homeostasis of hyaluronic acid in normal and scarred vocal folds.
Journal of voice : official journal of the Voice Foundation29(2)133-9(2015 Mar)
Tateya I,Tateya T,Watanuki M,Bless DM

Involvement of hyaluronan synthesis in ovarian follicle growth in rats.
Reproduction (Cambridge, England)147(2)189-97(2014 Feb)
Takahashi N,Tarumi W,Ishizuka B

Tissue distribution and subcellular localization of hyaluronan synthase isoenzymes.
Histochemistry and cell biology141(1)17-31(2014 Jan)
T?rr?nen K,Nikunen K,K?rn? R,Tammi M,Tammi R,Rilla K

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Has2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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