Gnai3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gnai3
Entrez Gene ID	25643
Full Name	G protein subunit alpha i3
Synonyms	GIP3A
General protein information	Preferred Names G protein subunit alpha i3 Names guanine nucleotide-binding protein G(k) subunit alpha Guanine nucleotide binding, protein, alpha inhibiting polypeptide 3 g(i) alpha-3 guanine nucleotide binding protein (G protein), alpha inhibiting 3 guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3 guanine nucleotide binding protein, alpha inhibiting 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	has GTPase activity; involved in zymogen granule-plasma membrane fusion in pancreatic cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013106.1	NP_037238.1	guanine nucleotide-binding protein G(i) subunit alpha

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GNAI3	XP_513624.1
	GNAI3	XP_001091470.1
Bos taurus (cattle)	GNAI3	NP_001029849.1
Rattus norvegicus (Norway rat)	Gnai3	NP_037238.1
Canis lupus familiaris (dog)	GNAI3	XP_854614.3
Xenopus tropicalis (tropical clawed frog)	gnai3	NP_001011471.1
Homo sapiens (human)	GNAI3	NP_006487.1
Danio rerio (zebrafish)	gnai3	NP_001104720.1
Mus musculus (house mouse)	Gnai3	NP_034436.1
Gallus gallus (chicken)	GNAI3	NP_989580.1

Gene Ontology
Bibliography

Related articles in PubMed

Nitric oxide attenuates overexpression of Gi? proteins in vascular smooth muscle cells from SHR: Role of ROS and ROS-mediated signaling.
Sarkar O, Li Y, Anand-Srivastava MB
PloS one12(7)e0179301(2017)

Knockdown of Inhibitory Guanine Nucleotide Binding Protein Gi?-2 by Antisense Oligodeoxynucleotides Attenuates the Development of Hypertension and Tachycardia in Spontaneously Hypertensive Rats.
Ali El-Basyuni Y, Li Y, Anand-Srivastava MB
Journal of the American Heart Association5(11)(2016 Oct)

GIV/Girdin activates G?i and inhibits G?s via the same motif.
Gupta V, Bhandari D, Leyme A, Aznar N, Midde KK, Lo IC, Ear J, Niesman I, L?pez-S?nchez I, Blanco-Canosa JB, von Zastrow M, Garcia-Marcos M, Farquhar MG, Ghosh P
Proceedings of the National Academy of Sciences of the United States of America113(39)E5721-30(2016 09)

Lysophosphatidic acid rescues bone mesenchymal stem cells from hydrogen peroxide-induced apoptosis.
Wang XY, Fan XS, Cai L, Liu S, Cong XF, Chen X
Apoptosis : an international journal on programmed cell death20(3)273-84(2015 Mar)

Natriuretic peptide receptor-C agonist attenuates the expression of cell cycle proteins and proliferation of vascular smooth muscle cells from spontaneously hypertensive rats: role of Gi proteins and MAPkinase/PI3kinase signaling.
El Andalousi J, Li Y, Anand-Srivastava MB
PloS one8(10)e76183(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nitric oxide decreased the enhanced expression of Gialpha-2/3 proteins and hyperproliferation of Vascular smooth muscle cells from spontaneously hypertensive rats by cGMP-independent mechanism.
Title: Nitric oxide attenuates overexpression of Gi? proteins in vascular smooth muscle cells from SHR: Role of ROS and ROS-mediated signaling.

These results indicate that NPR-C activation by C-ANP4-23 attenuates the enhanced levels of cell cycle proteins through the inhibition of enhanced expression of Gialpha proteins.
Title: Natriuretic peptide receptor-C agonist attenuates the expression of cell cycle proteins and proliferation of vascular smooth muscle cells from spontaneously hypertensive rats: role of Gi proteins and MAPkinase/PI3kinase signaling.

Results suggest that the suppression of Dopamine-2 (D2) receptor short (D2S)-regulated Gi3 inhibition of Gs protein may be one of the mechanisms controlling estradiol (E(2)-activated prolactin (PRL) synthesis and cell proliferation in lactotropes.
Title: Estrogen inhibits D2S receptor-regulated Gi3 and Gs protein interactions to stimulate prolactin production and cell proliferation in lactotropic cells.

Suggest role for oxidative stress in high glucose-induced decreased expression of Gnai2/3 and adenylyl cyclase signaling in vascular smooth muscle cells.
Title: Role of oxidative stress in high glucose-induced decreased expression of Gialpha proteins and adenylyl cyclase signaling in vascular smooth muscle cells.

studies suggest a possible role for zymogen granule-associated G(alpha)(i3) in zymogen granule-plasma membrane fusion
Title: G(alpha)(i3) in pancreatic zymogen granules participates in vesicular fusion.

The following Gnai3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gnai3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01999	NM_013106.1 Latest version!	Rattus norvegicus G protein subunit alpha i3 (Gnai3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gnai3 ( NM_013106.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037238.1 Rattus norvegicus G protein subunit alpha i3 (Gnai3), mRNA.

Price & Availability↑

CloneID	ORa01999
Clone ID Related Accession (Same CDS sequence)	NM_013106.1
Accession Version	NM_013106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	guanine nucleotide-binding protein G(i) subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M20713.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M20713.1, FQ222046.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGGCTGCA CGTTGAGCGC CGAGGACAAG GCGGCGGTGG AGCGGAGTAA GATGATCGAC 
CGCAACTTGC GGGAGGACGG AGAGAAAGCG GCCAAAGAAG TGAAGCTGCT GCTGCTCGGC 
GCTGGAGAAT CTGGTAAAAG TACTATTGTG AAACAGATGA AAATCATTCA TGAGGATGGC 
TATTCCGAGG ACGAGTGTAA GCAGTATAAA GTTGTCGTCT ACAGCAATAC CATTCAGTCC 
ATCATTGCAA TCATAAGAGC CATGGGACGG TTGAAGATTG ATTTTGGGGA AGCTGCCAGA 
GCGGATGATG CCCGACAGTT ATTTGTTTTA GCTGGCAGTG CTGAGGAAGG AGTCATGACT 
TCAGAACTAG CAGGCGTGAT TAAACGTTTA TGGCGAGATG GCGGGGTGCA GGCATGCTTC 
AGCAGGTCCA GGGAATATCA GCTCAATGAT TCTGCTTCAT ATTACCTAAA TGATTTGGAT 
AGAATATCCC AGACCAACTA CATTCCAACT CAGCAAGATG TTCTTCGGAC GAGAGTGAAG 
ACTACAGGCA TTGTGGAGAC CCACTTCACC TTCAAGGAGC TCTACTTCAA AATGTTTGAT 
GTAGGTGGCC AAAGATCCGA ACGGAAAAAG TGGATCCACT GTTTTGAGGG AGTGACAGCA 
ATTATCTTTT GTGTGGCTCT CAGTGATTAC GACCTTGTTC TGGCTGAGGA CGAGGAAATG 
AACCGAATGC ATGAAAGCAT GAAATTGTTT GACAGCATTT GTAACAACAA ATGGTTTACA 
GACACTTCAA TCATTCTCTT CCTTAATAAG AAAGACCTTT TTGAGGAAAA AATAAAGAGG 
AGTCCATTAA CAATCTGTTA TCCAGAATAC ACAGGTTCCA ATACGTACGA AGAGGCAGCT 
GCTTACATCC AGTGCCAGTT TGAAGATCTG AACCGAAGAA AGGACACCAA GGAGGTCTAC 
ACTCACTTTA CCTGTGCCAC TGACACCAAA AACGTGCAGT TTGTTTTTGA TGCTGTTACA 
GATGTCATCA TTAAAAACAA CTTAAAGGAA TGTGGGCTTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037238.1
CDS	85..1149
Translation	MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMTSELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQTNYIPTQQDVLRTRVKTTGIVETHFTFKELYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEVYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY

Target ORF information:

RefSeq Version	NM_013106.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus G protein subunit alpha i3 (Gnai3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013106.1

ORF Insert Sequence:

ATGGGCTGCA CGTTGAGCGC CGAGGACAAG GCGGCGGTGG AGCGGAGTAA GATGATCGAC 
CGCAACTTGC GGGAGGACGG AGAGAAAGCG GCCAAAGAAG TGAAGCTGCT GCTGCTCGGC 
GCTGGAGAAT CTGGTAAAAG TACTATTGTG AAACAGATGA AAATCATTCA TGAGGATGGC 
TATTCCGAGG ACGAGTGTAA GCAGTATAAA GTTGTCGTCT ACAGCAATAC CATTCAGTCC 
ATCATTGCAA TCATAAGAGC CATGGGACGG TTGAAGATTG ATTTTGGGGA AGCTGCCAGA 
GCGGATGATG CCCGACAGTT ATTTGTTTTA GCTGGCAGTG CTGAGGAAGG AGTCATGACT 
TCAGAACTAG CAGGCGTGAT TAAACGTTTA TGGCGAGATG GCGGGGTGCA GGCATGCTTC 
AGCAGGTCCA GGGAATATCA GCTCAATGAT TCTGCTTCAT ATTACCTAAA TGATTTGGAT 
AGAATATCCC AGACCAACTA CATTCCAACT CAGCAAGATG TTCTTCGGAC GAGAGTGAAG 
ACTACAGGCA TTGTGGAGAC CCACTTCACC TTCAAGGAGC TCTACTTCAA AATGTTTGAT 
GTAGGTGGCC AAAGATCCGA ACGGAAAAAG TGGATCCACT GTTTTGAGGG AGTGACAGCA 
ATTATCTTTT GTGTGGCTCT CAGTGATTAC GACCTTGTTC TGGCTGAGGA CGAGGAAATG 
AACCGAATGC ATGAAAGCAT GAAATTGTTT GACAGCATTT GTAACAACAA ATGGTTTACA 
GACACTTCAA TCATTCTCTT CCTTAATAAG AAAGACCTTT TTGAGGAAAA AATAAAGAGG 
AGTCCATTAA CAATCTGTTA TCCAGAATAC ACAGGTTCCA ATACGTACGA AGAGGCAGCT 
GCTTACATCC AGTGCCAGTT TGAAGATCTG AACCGAAGAA AGGACACCAA GGAGGTCTAC 
ACTCACTTTA CCTGTGCCAC TGACACCAAA AACGTGCAGT TTGTTTTTGA TGCTGTTACA 
GATGTCATCA TTAAAAACAA CTTAAAGGAA TGTGGGCTTT ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nitric oxide attenuates overexpression of Gi? proteins in vascular smooth muscle cells from SHR: Role of ROS and ROS-mediated signaling.
PloS one12(7)e0179301(2017)
Sarkar O,Li Y,Anand-Srivastava MB

Knockdown of Inhibitory Guanine Nucleotide Binding Protein Gi?-2 by Antisense Oligodeoxynucleotides Attenuates the Development of Hypertension and Tachycardia in Spontaneously Hypertensive Rats.
Journal of the American Heart Association5(11)(2016 Oct)
Ali El-Basyuni Y,Li Y,Anand-Srivastava MB

GIV/Girdin activates G?i and inhibits G?s via the same motif.
Proceedings of the National Academy of Sciences of the United States of America113(39)E5721-30(2016 09)
Gupta V,Bhandari D,Leyme A,Aznar N,Midde KK,Lo IC,Ear J,Niesman I,L?pez-S?nchez I,Blanco-Canosa JB,von Zastrow M,Garcia-Marcos M,Farquhar MG,Ghosh P

Lysophosphatidic acid rescues bone mesenchymal stem cells from hydrogen peroxide-induced apoptosis.
Apoptosis : an international journal on programmed cell death20(3)273-84(2015 Mar)
Wang XY,Fan XS,Cai L,Liu S,Cong XF,Chen X

Natriuretic peptide receptor-C agonist attenuates the expression of cell cycle proteins and proliferation of vascular smooth muscle cells from spontaneously hypertensive rats: role of Gi proteins and MAPkinase/PI3kinase signaling.
PloS one8(10)e76183(2013)
El Andalousi J,Li Y,Anand-Srivastava MB

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Gnai3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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