Ube2i cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2i
Entrez Gene ID	25573
Full Name	ubiquitin-conjugating enzyme E2I
Synonyms	UbcE2A
General protein information	Preferred Names ubiquitin-conjugating enzyme E2I Names SUMO-conjugating enzyme UBC9 RING-type E3 SUMO transferase UBC9 SUMO-protein ligase Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) ubiquitin carrier protein 9 ubiquitin carrier protein I ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) ubiquitin-conjugating enzyme UbcE2A ubiquitin-protein ligase I
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12
Summary	ubuiquitin-conjugating enzyme (E2 class), catalyzes the attachment of ubiquitin-like protein Sumo1 to other proteins; may be involved in the regulation of cell growth and differentiation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006245920.3	XP_006245982.1	SUMO-conjugating enzyme UBC9 isoform X1
NM_013050.1	NP_037182.1	SUMO-conjugating enzyme UBC9
XM_006245919.3	XP_006245981.1	SUMO-conjugating enzyme UBC9 isoform X1
XM_006245918.3	XP_006245980.2	SUMO-conjugating enzyme UBC9 isoform X1
XM_008767549.2	XP_008765771.2	SUMO-conjugating enzyme UBC9 isoform X1
XM_017597044.1	XP_017452533.1	SUMO-conjugating enzyme UBC9 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE2I	NP_003336.1
Pan troglodytes (chimpanzee)	UBE2I	XP_003314955.1
	UBE2I	XP_002802397.1
Bos taurus (cattle)	UBE2I	NP_001092842.1
Rattus norvegicus (Norway rat)	Ube2i	NP_037182.1
Gallus gallus (chicken)	UBE2I	NP_989596.1
Saccharomyces cerevisiae (baker's yeast)	UBC9	NP_010219.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011076	XP_309574.1
Oryza sativa (rice)	Os03g0123100	NP_001048802.1
Oryza sativa (rice)	Os10g0536000	NP_001065167.1
Mus musculus (house mouse)	Ube2i	NP_035795.1
Danio rerio (zebrafish)	ube2i2	NP_571908.1
Eremothecium gossypii	AGOS_AER056C	NP_984916.1
Schizosaccharomyces pombe (fission yeast)	hus5	NP_593204.1
Arabidopsis thaliana (thale cress)	SCE1	NP_191346.1
Xenopus tropicalis (tropical clawed frog)	ube2i	NP_001016408.1
Drosophila melanogaster (fruit fly)	lwr	NP_001259833.1
Kluyveromyces lactis	KLLA0E05039g	XP_454172.1
Magnaporthe oryzae (rice blast fungus)	MGG_00970	XP_003717980.1
Canis lupus familiaris (dog)	UBE2I	XP_005621791.1
Neurospora crassa	NCU04302	XP_961092.1

Gene Ontology
Bibliography

Related articles in PubMed

The C-terminal Region and SUMOylation of Cockayne Syndrome Group B Protein Play Critical Roles in Transcription-coupled Nucleotide Excision Repair.
Sin Y, Tanaka K, Saijo M
The Journal of biological chemistry291(3)1387-97(2016 Jan)

TWIST1 regulates the activity of ubiquitin proteasome system via the miR-199/214 cluster in human end-stage dilated cardiomyopathy.
Baumgarten A, Bang C, Tschirner A, Engelmann A, Adams V, von Haehling S, Doehner W, Pregla R, Anker MS, Blecharz K, Meyer R, Hetzer R, Anker SD, Thum T, Springer J
International journal of cardiology168(2)1447-52(2013 Sep)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Interactions of the mineralocorticoid receptor--within and without.
Yang J, Fuller PJ
Molecular and cellular endocrinology350(2)196-205(2012 Mar)

Ribosomal protein S3 is stabilized by sumoylation.
Jang CY, Shin HS, Kim HD, Kim JW, Choi SY, Kim J
Biochemical and biophysical research communications414(3)523-7(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ISH analysis revealed expression of Ubc9 was high in neuronal stem cells and moderate in differentiated neurons at embryonic stages but in adult brain, moderate expression was observed in dentate granular neurons and pyramidal neurons
Title: Developmental regulation of Ubc9 in the rat nervous system.

Immunohistochemistry showed that Ubc9 and PIAS1 are markedly expressed in rat adrenal glomerulosa cells
Title: Ubc9 and Protein Inhibitor of Activated STAT 1 Activate Chicken Ovalbumin Upstream Promoter-Transcription Factor I-mediated Human CYP11B2 Gene Transcription.

Glucocorticoid receptor ligand binding domain is sufficient for the modulation of glucocorticoid induction properties by homologous receptors, coactivator transcription intermediary factor 2, and Ubc9
Title: Glucocorticoid receptor ligand binding domain is sufficient for the modulation of glucocorticoid induction properties by homologous receptors, coactivator transcription intermediary factor 2, and Ubc9.

The following Ube2i gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2i cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01779	NM_013050.1 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa01779	XM_006245920.3 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa01779	XM_008767549.2 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa01779	XM_006245918.3 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa01779	XM_017597044.1 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa01779	XM_006245919.3 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2i ( NM_013050.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037182.1 Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), mRNA.

Price & Availability↑

CloneID	ORa01779
Clone ID Related Accession (Same CDS sequence)	XM_006245920.3 , NM_013050.1 , XM_006245919.3 , XM_006245918.3 , XM_008767549.2 , XM_017597044.1
Accession Version	NM_013050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Rattus norvegicus(Norway rat)
Product	SUMO-conjugating enzyme UBC9
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U54632.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U54632.1, CF110316.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037182.1
CDS	82..558
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	NM_013050.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013050.1

ORF Insert Sequence:

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2i ( XM_006245920.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245982.1 Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa01779
Clone ID Related Accession (Same CDS sequence)	XM_006245920.3 , NM_013050.1 , XM_006245919.3 , XM_006245918.3 , XM_008767549.2 , XM_017597044.1
Accession Version	XM_006245920.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SUMO-conjugating enzyme UBC9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245920.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245982.1
CDS	157..633
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	XM_006245920.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245920.3

ORF Insert Sequence:

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2i ( XM_006245919.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245981.1 Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa01779
Clone ID Related Accession (Same CDS sequence)	XM_006245920.3 , NM_013050.1 , XM_006245919.3 , XM_006245918.3 , XM_008767549.2 , XM_017597044.1
Accession Version	XM_006245919.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SUMO-conjugating enzyme UBC9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245919.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245981.1
CDS	756..1232
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	XM_006245919.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245919.3

ORF Insert Sequence:

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2i ( XM_006245918.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245980.2 Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa01779
Clone ID Related Accession (Same CDS sequence)	XM_006245920.3 , NM_013050.1 , XM_006245919.3 , XM_006245918.3 , XM_008767549.2 , XM_017597044.1
Accession Version	XM_006245918.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SUMO-conjugating enzyme UBC9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245918.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245980.2
CDS	71..547
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	XM_006245918.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245918.3

ORF Insert Sequence:

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2i ( XM_008767549.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765771.2 Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01779
Clone ID Related Accession (Same CDS sequence)	XM_006245920.3 , NM_013050.1 , XM_006245919.3 , XM_006245918.3 , XM_008767549.2 , XM_017597044.1
Accession Version	XM_008767549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SUMO-conjugating enzyme UBC9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765771.2
CDS	165..641
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	XM_008767549.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767549.2

ORF Insert Sequence:

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2i ( XM_017597044.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452533.1 Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X5, mRNA.

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CloneID	ORa01779
Clone ID Related Accession (Same CDS sequence)	XM_006245920.3 , NM_013050.1 , XM_006245919.3 , XM_006245918.3 , XM_008767549.2 , XM_017597044.1
Accession Version	XM_017597044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SUMO-conjugating enzyme UBC9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452533.1
CDS	236..712
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	XM_017597044.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597044.1

ORF Insert Sequence:

ATGTCGGGGA TTGCCCTCAG CCGACTTGCG CAGGAGAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTGGCTGT CCCAACAAAG AACCCTGATG GCACGATGAA CCTGATGAAC 
TGGGAGTGTG CTATCCCTGG AAAGAAGGGG ACTCCGTGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCACCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGC GGCCAGCTAT TACGATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ATACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTTCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The C-terminal Region and SUMOylation of Cockayne Syndrome Group B Protein Play Critical Roles in Transcription-coupled Nucleotide Excision Repair.
The Journal of biological chemistry291(3)1387-97(2016 Jan)
Sin Y,Tanaka K,Saijo M

TWIST1 regulates the activity of ubiquitin proteasome system via the miR-199/214 cluster in human end-stage dilated cardiomyopathy.
International journal of cardiology168(2)1447-52(2013 Sep)
Baumgarten A,Bang C,Tschirner A,Engelmann A,Adams V,von Haehling S,Doehner W,Pregla R,Anker MS,Blecharz K,Meyer R,Hetzer R,Anker SD,Thum T,Springer J

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Interactions of the mineralocorticoid receptor--within and without.
Molecular and cellular endocrinology350(2)196-205(2012 Mar)
Yang J,Fuller PJ

Ribosomal protein S3 is stabilized by sumoylation.
Biochemical and biophysical research communications414(3)523-7(2011 Oct)
Jang CY,Shin HS,Kim HD,Kim JW,Choi SY,Kim J

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Ube2i cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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