Ube2i cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2i
Entrez Gene ID	22196
Full Name	ubiquitin-conjugating enzyme E2I
Synonyms	5830467E05Rik,UBC9,Ubce2i,Ubce9
General protein information	Preferred Names ubiquitin-conjugating enzyme E2I Names SUMO-conjugating enzyme UBC9 RING-type E3 SUMO transferase UBC9 SUMO-protein ligase ubiquitin carrier protein 9 ubiquitin carrier protein I ubiquitin-protein ligase I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 A3.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001177610.1	NP_001171081.1	SUMO-conjugating enzyme UBC9
XM_006524049.4	XP_006524112.1	SUMO-conjugating enzyme UBC9 isoform X1
NM_001357694.1	NP_001344623.1	SUMO-conjugating enzyme UBC9
NM_011665.4	NP_035795.1	SUMO-conjugating enzyme UBC9
XM_006524049.3	XP_006524112.1	SUMO-conjugating enzyme UBC9 isoform X1
XM_006524048.3	XP_006524111.1	SUMO-conjugating enzyme UBC9 isoform X1
NM_001177609.1	NP_001171080.1	SUMO-conjugating enzyme UBC9

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE2I	NP_003336.1
Pan troglodytes (chimpanzee)	UBE2I	XP_003314955.1
	UBE2I	XP_002802397.1
Bos taurus (cattle)	UBE2I	NP_001092842.1
Rattus norvegicus (Norway rat)	Ube2i	NP_037182.1
Gallus gallus (chicken)	UBE2I	NP_989596.1
Saccharomyces cerevisiae (baker's yeast)	UBC9	NP_010219.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011076	XP_309574.1
Oryza sativa (rice)	Os03g0123100	NP_001048802.1
Oryza sativa (rice)	Os10g0536000	NP_001065167.1
Mus musculus (house mouse)	Ube2i	NP_035795.1
Danio rerio (zebrafish)	ube2i2	NP_571908.1
Eremothecium gossypii	AGOS_AER056C	NP_984916.1
Schizosaccharomyces pombe (fission yeast)	hus5	NP_593204.1
Arabidopsis thaliana (thale cress)	SCE1	NP_191346.1
Xenopus tropicalis (tropical clawed frog)	ube2i	NP_001016408.1
Drosophila melanogaster (fruit fly)	lwr	NP_001259833.1
Kluyveromyces lactis	KLLA0E05039g	XP_454172.1
Magnaporthe oryzae (rice blast fungus)	MGG_00970	XP_003717980.1
Canis lupus familiaris (dog)	UBE2I	XP_005621791.1
Neurospora crassa	NCU04302	XP_961092.1

Gene Ontology
Bibliography

Related articles in PubMed

Wang A, Ding X, Demarque M, Liu X, Pan D, Xin H, Zhong B, Wang X, Dejean A, Jin W, Dong C
Journal of immunology (Baltimore, Md. : 1950)198(9)3461-3470(2017 May)

Manipulating PML SUMOylation via Silencing UBC9 and RNF4 Regulates Cardiac Fibrosis.
Liu Y, Zhao D, Qiu F, Zhang LL, Liu SK, Li YY, Liu MT, Wu D, Wang JX, Ding XQ, Liu YX, Dong CJ, Shao XQ, Yang BF, Chu WF
Molecular therapy : the journal of the American Society of Gene Therapy25(3)666-678(2017 Mar)

Nur77 suppresses hepatocellular carcinoma via switching glucose metabolism toward gluconeogenesis through attenuating phosphoenolpyruvate carboxykinase sumoylation.
Bian XL, Chen HZ, Yang PB, Li YP, Zhang FN, Zhang JY, Wang WJ, Zhao WX, Zhang S, Chen QT, Zheng Y, Sun XY, Wang XM, Chien KY, Wu Q
Nature communications814420(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that loss of Ubc9 results in a significant reduction of CD4 and CD8 single-positive lymphocytes in both thymus and periphery

These findings suggest that the UBC9/PML/RNF4 axis plays a critical role as an important SUMO pathway in cardiac fibrosis. Modulating the protein levels of the pathway provides an attractive therapeutic target for the treatment of cardiac fibrosis and heart failure.
Title: Manipulating PML SUMOylation via Silencing UBC9 and RNF4 Regulates Cardiac Fibrosis.

Increased UBC9-mediated SUMOylation is sufficient to upregulate cardiac autophagy. UBC9 overexpression reduced aggregate formation, decreased fibrosis, reduced hypertrophy, and improved cardiac function and survival.
Title: UBC9-Mediated Sumoylation Favorably Impacts Cardiac Function in Compromised Hearts.

The study reveals a function of SUMO protein modification as a Ubiquitin-independent ESCRT sorting signal, regulating the extracellular vesicle release of alpha-Synuclein.
Title: Extracellular vesicle sorting of ?-Synuclein is regulated by sumoylation.

Synaptic trapping of Ubc9 through a PKC phosphorylation-dependent increase of Ubc9 recognition to phosphorylated substrates and consequently leads to the modulation of synaptic sumoylation.
Title: mGlu5 receptors regulate synaptic sumoylation via a transient PKC-dependent diffusional trapping of Ubc9 into spines.

The following Ube2i gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2i cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00624	NM_001357694.1 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu00624	NM_001177609.1 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu00624	NM_011665.4 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu00624	NM_001177610.1 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu00624	XM_006524048.3 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu00624	XM_006524049.4 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu00624	XM_006524049.3	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2i ( NM_001177610.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171081.1 Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu00624
Clone ID Related Accession (Same CDS sequence)	NM_001177610.1 , XM_006524049.4 , NM_001357694.1 , NM_011665.4 , XM_006524049.3 , XM_006524048.3 , NM_001177609.1
Accession Version	NM_001177610.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Mus musculus(house mouse)
Product	SUMO-conjugating enzyme UBC9
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122454.4 and AK166930.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 2. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK160988.1, SRR1660811.157757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171081.1
CDS	123..599
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	NM_001177610.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177610.1

ORF Insert Sequence:

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2i ( NM_001357694.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001344623.1 Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu00624
Clone ID Related Accession (Same CDS sequence)	NM_001177610.1 , XM_006524049.4 , NM_001357694.1 , NM_011665.4 , XM_006524049.3 , XM_006524048.3 , NM_001177609.1
Accession Version	NM_001357694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	SUMO-conjugating enzyme UBC9
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122454.4 and CD776278.1. On Nov 4, 2017 this sequence version replaced XM_006524048.3. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 2. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.26526.1, CF894562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001344623.1
CDS	125..601
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	NM_001357694.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001357694.1

ORF Insert Sequence:

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2i ( NM_011665.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035795.1 Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu00624
Clone ID Related Accession (Same CDS sequence)	NM_001177610.1 , XM_006524049.4 , NM_001357694.1 , NM_011665.4 , XM_006524049.3 , XM_006524048.3 , NM_001177609.1
Accession Version	NM_011665.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Mus musculus(house mouse)
Product	SUMO-conjugating enzyme UBC9
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY074880.1 and AK005058.1. On Apr 20, 2010 this sequence version replaced NM_011665.3. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK005058.1, U94402.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035795.1
CDS	161..637
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	NM_011665.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011665.4

ORF Insert Sequence:

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2i ( NM_001177609.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171080.1 Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu00624
Clone ID Related Accession (Same CDS sequence)	NM_001177610.1 , XM_006524049.4 , NM_001357694.1 , NM_011665.4 , XM_006524049.3 , XM_006524048.3 , NM_001177609.1
Accession Version	NM_001177609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	SUMO-conjugating enzyme UBC9
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK166657.1, AK150575.1 and AK166930.1. Transcript Variant: This variant (2) represents the longest transcript. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.43562.1, SRR1660817.314111.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164142 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171080.1
CDS	834..1310
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	NM_001177609.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177609.1

ORF Insert Sequence:

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2i ( XM_006524049.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524112.1 Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu00624
Clone ID Related Accession (Same CDS sequence)	NM_001177610.1 , XM_006524049.4 , NM_001357694.1 , NM_011665.4 , XM_006524049.3 , XM_006524048.3 , NM_001177609.1
Accession Version	XM_006524049.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	SUMO-conjugating enzyme UBC9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524112.1
CDS	150..626
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	XM_006524049.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524049.3

ORF Insert Sequence:

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2i ( XM_006524048.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524111.1 Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X1, mRNA.

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CloneID	OMu00624
Clone ID Related Accession (Same CDS sequence)	NM_001177610.1 , XM_006524049.4 , NM_001357694.1 , NM_011665.4 , XM_006524049.3 , XM_006524048.3 , NM_001177609.1
Accession Version	XM_006524048.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	SUMO-conjugating enzyme UBC9 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006524048.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524111.1
CDS	396..872
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	XM_006524048.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524048.3

ORF Insert Sequence:

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2i ( XM_006524049.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006524112.1 Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu00624
Clone ID Related Accession (Same CDS sequence)	NM_001177610.1 , XM_006524049.4 , NM_001357694.1 , NM_011665.4 , XM_006524049.3 , XM_006524048.3 , NM_001177609.1
Accession Version	XM_006524049.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	SUMO-conjugating enzyme UBC9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006524049.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006524112.1
CDS	1639..2115
Translation	MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Target ORF information:

RefSeq Version	XM_006524049.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2I (Ube2i), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006524049.4

ORF Insert Sequence:

ATGTCGGGGA TCGCCCTCAG CCGCCTTGCG CAGGAAAGGA AAGCCTGGAG GAAGGACCAC 
CCTTTTGGCT TTGTAGCTGT CCCAACAAAG AACCCTGATG GCACAATGAA CCTGATGAAC 
TGGGAGTGCG CTATCCCTGG AAAGAAGGGG ACTCCATGGG AAGGAGGCTT GTTCAAGCTA 
CGGATGCTTT TCAAAGATGA CTATCCGTCC TCACCACCAA AATGTAAATT TGAGCCCCCA 
CTGTTTCATC CAAACGTGTA TCCTTCTGGC ACAGTGTGCC TGTCCATCCT GGAGGAAGAC 
AAGGACTGGA GGCCAGCTAT CACCATCAAA CAGATCTTAT TAGGAATACA AGAACTTCTA 
AATGAACCAA ATATTCAAGA CCCAGCTCAA GCAGAGGCCT ACACAATTTA CTGCCAAAAC 
AGAGTGGAAT ATGAGAAAAG GGTCCGAGCA CAAGCGAAGA AGTTTGCCCC CTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Journal of immunology (Baltimore, Md. : 1950)198(9)3461-3470(2017 May)
Wang A,Ding X,Demarque M,Liu X,Pan D,Xin H,Zhong B,Wang X,Dejean A,Jin W,Dong C

Manipulating PML SUMOylation via Silencing UBC9 and RNF4 Regulates Cardiac Fibrosis.
Molecular therapy : the journal of the American Society of Gene Therapy25(3)666-678(2017 Mar)
Liu Y,Zhao D,Qiu F,Zhang LL,Liu SK,Li YY,Liu MT,Wu D,Wang JX,Ding XQ,Liu YX,Dong CJ,Shao XQ,Yang BF,Chu WF

Nur77 suppresses hepatocellular carcinoma via switching glucose metabolism toward gluconeogenesis through attenuating phosphoenolpyruvate carboxykinase sumoylation.
Nature communications814420(2017 Feb)
Bian XL,Chen HZ,Yang PB,Li YP,Zhang FN,Zhang JY,Wang WJ,Zhao WX,Zhang S,Chen QT,Zheng Y,Sun XY,Wang XM,Chien KY,Wu Q

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Ube2i cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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