gRNAs
The following Bfsp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bfsp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa60272 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006235117.3 | |
| Accession Version | XM_006235117.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | filensin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235117.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGGACGT GGTGCTCAGA CTTGGTTCTC CTGGGCTCTT TTGAGGGTAT TTGTGCTGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006235179.2 |
| CDS | 239..1969 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_006235117.3 |
1 | ATGAGGACGT GGTGCTCAGA CTTGGTTCTC CTGGGCTCTT TTGAGGGTAT TTGTGCTGCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa60273 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017591490.1 | |
| Accession Version | XM_017591490.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1626bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | filensin isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGACAAAAC AAACTGAGCC CCAGTTTGTC CTTCTGCCCC GCTGCTTCCT GCAGATGTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017446979.1 |
| CDS | 1332..2957 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017591490.1 |
1 | ATGACAAAAC AAACTGAGCC CCAGTTTGTC CTTCTGCCCC GCTGCTTCCT GCAGATGTGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa08091 | |
| Clone ID Related Accession (Same CDS sequence) | NM_031555.1 | |
| Accession Version | NM_031555.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | filensin | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB003104.1. On or before Oct 13, 2007 this sequence version replaced XM_001053697.1, XM_342529.2. ##Evidence-Data-START## Transcript exon combination :: AB003104.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMN01906347 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGTACCGCC GCAGCTACGT CTTCCAGGCC CGCCAGGAGC GCTACGAGCG CGCCCAGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_113743.1 |
| CDS | 13..2007 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_031555.1 |
1 | ATGTACCGCC GCAGCTACGT CTTCCAGGCC CGCCAGGAGC GCTACGAGCG CGCCCAGCCC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The function of filensin and phakinin in lens transparency. |
A role for lengsin, a recruited enzyme, in terminal differentiation in the vertebrate lens. |
Resisting the effects of aging: a function for the fiber cell beaded filament. |
Bfsp2 mutation found in mouse 129 strains causes the loss of CP49' and induces vimentin-dependent changes in the lens fibre cell cytoskeleton. |
Analysis of non-crystallin lens fiber cell gene expression in c-Maf -/- mice. |
