Prnp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prnp
Entrez Gene ID	24686
Full Name	prion protein
Synonyms	PrP,Prn
General protein information	Preferred Names prion protein Names major prion protein prion protein, PrP prion protein, structural
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q36
Summary	conformational conversion of gene product prion protein (PrP) associated with Prion diseases, fatal neurodegenerative disorders in man and animal [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012631.2	NP_036763.1	major prion protein precursor
XM_006235062.3	XP_006235124.1	major prion protein isoform X1

Pathways from BioSystems

Homologies

	PRNP	NP_001040617.1
Bos taurus (cattle)	PRNP	NP_851358.2
Mus musculus (house mouse)	Prnp	NP_001265185.1
Pan troglodytes (chimpanzee)	PRNP	NP_001009093.1
Rattus norvegicus (Norway rat)	Prnp	NP_036763.1
Homo sapiens (human)	PRNP	NP_898902.1

Gene Ontology
Bibliography

Related articles in PubMed

Morphine Withdrawal Modifies Prion Protein Expression in Rat Hippocampus.
Mattei V, Martellucci S, Santilli F, Manganelli V, Garofalo T, Candelise N, Caruso A, Sorice M, Scaccianoce S, Misasi R
PloS one12(1)e0169571(2017)

The prion protein selectively binds to and modulates the content of purinergic receptor P2X4R.
Carneiro MV, Americo TA, Guimar?es MZ, Linden R
Biochemical and biophysical research communications472(2)293-8(2016 Apr)

Regulators of Iron Homeostasis: New Players in Metabolism, Cell Death, and Disease.
Bogdan AR, Miyazawa M, Hashimoto K, Tsuji Y
Trends in biochemical sciences41(3)274-86(2016 Mar)

Stabilization of microtubular cytoskeleton protects neurons from toxicity of N-terminal fragment of cytosolic prion protein.
Zajkowski T, Nieznanska H, Nieznanski K
Biochimica et biophysica acta1853(10 Pt A)2228-39(2015 Oct)

Primary blast-induced traumatic brain injury in rats leads to increased prion protein in plasma: a potential biomarker for blast-induced traumatic brain injury.
Pham N, Sawyer TW, Wang Y, Jazii FR, Vair C, Taghibiglou C
Journal of neurotrauma32(1)58-65(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the mechanism by which stressful stimuli induced by opiate withdrawal and the subsequent long-term homeostatic changes in hippocampal plasticity, modulate the expression and the dynamics of Prion protein.
Title: Morphine Withdrawal Modifies Prion Protein Expression in Rat Hippocampus.

The results suggest a role of PrP(C) in proteostasis, dysfunctions of which may be involved in the pathogenesis of neurodegenerative diseases such as TSE and Alzheimer's Disease.
Title: The prion protein selectively binds to and modulates the content of purinergic receptor P2X4R.

this study implies that lithium, the commonly used mood stabilizer, may be a promising therapeutic agent in TSE, particularly in case of the disease forms associated with accumulation of cytoPrP.
Title: Stabilization of microtubular cytoskeleton protects neurons from toxicity of N-terminal fragment of cytosolic prion protein.

We provide the first report that mean PrPC concentration in primary blast exposed rats is significantly increased compared with controls
Title: Primary blast-induced traumatic brain injury in rats leads to increased prion protein in plasma: a potential biomarker for blast-induced traumatic brain injury.

data provide new insights into the cellular mechanism of prion conversion and suggest that GM1-prion protein interaction at the cell surface could play a significant role in the mechanism predisposing to pathology.
Title: Role of lipid rafts and GM1 in the segregation and processing of prion protein.

The following Prnp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prnp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00500	NM_012631.2 Latest version!	Rattus norvegicus prion protein (Prnp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
ORa00500	XM_006235062.3 Latest version!	Rattus norvegicus prion protein (Prnp), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prnp ( NM_012631.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036763.1 Rattus norvegicus prion protein (Prnp), mRNA.

Price & Availability↑

CloneID	ORa00500
Clone ID Related Accession (Same CDS sequence)	NM_012631.2 , XM_006235062.3
Accession Version	NM_012631.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	major prion protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D50093.2. On or before Dec 17, 2003 this sequence version replaced XM_346677.1, NM_012631.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ148608.1, CO394130.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAACC TTGGCTACTG GCTGCTGGCC CTCTTTGTGA CTACATGTAC TGATGTTGGC 
CTCTGCAAAA AGCGGCCAAA GCCTGGAGGG TGGAACACTG GTGGAAGCCG GTACCCTGGG 
CAGGGAAGCC CTGGAGGCAA CCGTTACCCA CCTCAGAGTG GTGGTACCTG GGGGCAGCCC 
CATGGTGGTG GCTGGGGACA ACCTCATGGT GGTGGCTGGG GACAACCTCA TGGTGGTGGC 
TGGGGTCAGC CCCATGGCGG GGGCTGGAGT CAAGGAGGGG GTACCCATAA TCAGTGGAAC 
AAGCCCAGCA AGCCAAAAAC CAACCTCAAG CATGTGGCAG GGGCTGCCGC AGCTGGGGCA 
GTAGTGGGGG GCCTTGGTGG CTACATGTTG GGGAGTGCCA TGAGCAGGCC CATGCTCCAT 
TTTGGCAACG ACTGGGAGGA CCGCTACTAC CGAGAAAACA TGTACCGTTA CCCTAACCAA 
GTGTACTACA GGCCGGTGGA TCAGTACAGC AACCAGAACA ACTTCGTGCA CGACTGTGTC 
AATATCACCA TCAAGCAGCA TACAGTCACC ACCACCACCA AGGGGGAGAA CTTCACGGAG 
ACCGACGTGA AGATGATGGA GCGTGTGGTG GAGCAGATGT GCGTCACCCA GTATCAGAAG 
GAGTCCCAGG CCTATTACGA CGGGAGAAGA TCTAGCGCCG TGCTTTTCTC CTCCCCTCCT 
GTGATCCTCC TCATCTCCTT CCTCATCTTC CTGATCGTGG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036763.1
CDS	156..920
Translation	MANLGYWLLALFVTTCTDVGLCKKRPKPGGWNTGGSRYPGQGSPGGNRYPPQSGGTWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWSQGGGTHNQWNKPSKPKTNLKHVAGAAAAGAVVGGLGGYMLGSAMSRPMLHFGNDWEDRYYRENMYRYPNQVYYRPVDQYSNQNNFVHDCVNITIKQHTVTTTTKGENFTETDVKMMERVVEQMCVTQYQKESQAYYDGRRSSAVLFSSPPVILLISFLIFLIVG

Target ORF information:

RefSeq Version	NM_012631.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prion protein (Prnp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012631.2

ORF Insert Sequence:

ATGGCGAACC TTGGCTACTG GCTGCTGGCC CTCTTTGTGA CTACATGTAC TGATGTTGGC 
CTCTGCAAAA AGCGGCCAAA GCCTGGAGGG TGGAACACTG GTGGAAGCCG GTACCCTGGG 
CAGGGAAGCC CTGGAGGCAA CCGTTACCCA CCTCAGAGTG GTGGTACCTG GGGGCAGCCC 
CATGGTGGTG GCTGGGGACA ACCTCATGGT GGTGGCTGGG GACAACCTCA TGGTGGTGGC 
TGGGGTCAGC CCCATGGCGG GGGCTGGAGT CAAGGAGGGG GTACCCATAA TCAGTGGAAC 
AAGCCCAGCA AGCCAAAAAC CAACCTCAAG CATGTGGCAG GGGCTGCCGC AGCTGGGGCA 
GTAGTGGGGG GCCTTGGTGG CTACATGTTG GGGAGTGCCA TGAGCAGGCC CATGCTCCAT 
TTTGGCAACG ACTGGGAGGA CCGCTACTAC CGAGAAAACA TGTACCGTTA CCCTAACCAA 
GTGTACTACA GGCCGGTGGA TCAGTACAGC AACCAGAACA ACTTCGTGCA CGACTGTGTC 
AATATCACCA TCAAGCAGCA TACAGTCACC ACCACCACCA AGGGGGAGAA CTTCACGGAG 
ACCGACGTGA AGATGATGGA GCGTGTGGTG GAGCAGATGT GCGTCACCCA GTATCAGAAG 
GAGTCCCAGG CCTATTACGA CGGGAGAAGA TCTAGCGCCG TGCTTTTCTC CTCCCCTCCT 
GTGATCCTCC TCATCTCCTT CCTCATCTTC CTGATCGTGG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prnp ( XM_006235062.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235124.1 Rattus norvegicus prion protein (Prnp), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa00500
Clone ID Related Accession (Same CDS sequence)	NM_012631.2 , XM_006235062.3
Accession Version	XM_006235062.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	major prion protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235062.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAACC TTGGCTACTG GCTGCTGGCC CTCTTTGTGA CTACATGTAC TGATGTTGGC 
CTCTGCAAAA AGCGGCCAAA GCCTGGAGGG TGGAACACTG GTGGAAGCCG GTACCCTGGG 
CAGGGAAGCC CTGGAGGCAA CCGTTACCCA CCTCAGAGTG GTGGTACCTG GGGGCAGCCC 
CATGGTGGTG GCTGGGGACA ACCTCATGGT GGTGGCTGGG GACAACCTCA TGGTGGTGGC 
TGGGGTCAGC CCCATGGCGG GGGCTGGAGT CAAGGAGGGG GTACCCATAA TCAGTGGAAC 
AAGCCCAGCA AGCCAAAAAC CAACCTCAAG CATGTGGCAG GGGCTGCCGC AGCTGGGGCA 
GTAGTGGGGG GCCTTGGTGG CTACATGTTG GGGAGTGCCA TGAGCAGGCC CATGCTCCAT 
TTTGGCAACG ACTGGGAGGA CCGCTACTAC CGAGAAAACA TGTACCGTTA CCCTAACCAA 
GTGTACTACA GGCCGGTGGA TCAGTACAGC AACCAGAACA ACTTCGTGCA CGACTGTGTC 
AATATCACCA TCAAGCAGCA TACAGTCACC ACCACCACCA AGGGGGAGAA CTTCACGGAG 
ACCGACGTGA AGATGATGGA GCGTGTGGTG GAGCAGATGT GCGTCACCCA GTATCAGAAG 
GAGTCCCAGG CCTATTACGA CGGGAGAAGA TCTAGCGCCG TGCTTTTCTC CTCCCCTCCT 
GTGATCCTCC TCATCTCCTT CCTCATCTTC CTGATCGTGG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235124.1
CDS	313..1077
Translation	MANLGYWLLALFVTTCTDVGLCKKRPKPGGWNTGGSRYPGQGSPGGNRYPPQSGGTWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWSQGGGTHNQWNKPSKPKTNLKHVAGAAAAGAVVGGLGGYMLGSAMSRPMLHFGNDWEDRYYRENMYRYPNQVYYRPVDQYSNQNNFVHDCVNITIKQHTVTTTTKGENFTETDVKMMERVVEQMCVTQYQKESQAYYDGRRSSAVLFSSPPVILLISFLIFLIVG

Target ORF information:

RefSeq Version	XM_006235062.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prion protein (Prnp), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235062.3

ORF Insert Sequence:

ATGGCGAACC TTGGCTACTG GCTGCTGGCC CTCTTTGTGA CTACATGTAC TGATGTTGGC 
CTCTGCAAAA AGCGGCCAAA GCCTGGAGGG TGGAACACTG GTGGAAGCCG GTACCCTGGG 
CAGGGAAGCC CTGGAGGCAA CCGTTACCCA CCTCAGAGTG GTGGTACCTG GGGGCAGCCC 
CATGGTGGTG GCTGGGGACA ACCTCATGGT GGTGGCTGGG GACAACCTCA TGGTGGTGGC 
TGGGGTCAGC CCCATGGCGG GGGCTGGAGT CAAGGAGGGG GTACCCATAA TCAGTGGAAC 
AAGCCCAGCA AGCCAAAAAC CAACCTCAAG CATGTGGCAG GGGCTGCCGC AGCTGGGGCA 
GTAGTGGGGG GCCTTGGTGG CTACATGTTG GGGAGTGCCA TGAGCAGGCC CATGCTCCAT 
TTTGGCAACG ACTGGGAGGA CCGCTACTAC CGAGAAAACA TGTACCGTTA CCCTAACCAA 
GTGTACTACA GGCCGGTGGA TCAGTACAGC AACCAGAACA ACTTCGTGCA CGACTGTGTC 
AATATCACCA TCAAGCAGCA TACAGTCACC ACCACCACCA AGGGGGAGAA CTTCACGGAG 
ACCGACGTGA AGATGATGGA GCGTGTGGTG GAGCAGATGT GCGTCACCCA GTATCAGAAG 
GAGTCCCAGG CCTATTACGA CGGGAGAAGA TCTAGCGCCG TGCTTTTCTC CTCCCCTCCT 
GTGATCCTCC TCATCTCCTT CCTCATCTTC CTGATCGTGG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Morphine Withdrawal Modifies Prion Protein Expression in Rat Hippocampus.
PloS one12(1)e0169571(2017)
Mattei V,Martellucci S,Santilli F,Manganelli V,Garofalo T,Candelise N,Caruso A,Sorice M,Scaccianoce S,Misasi R

The prion protein selectively binds to and modulates the content of purinergic receptor P2X4R.
Biochemical and biophysical research communications472(2)293-8(2016 Apr)
Carneiro MV,Americo TA,Guimar?es MZ,Linden R

Regulators of Iron Homeostasis: New Players in Metabolism, Cell Death, and Disease.
Trends in biochemical sciences41(3)274-86(2016 Mar)
Bogdan AR,Miyazawa M,Hashimoto K,Tsuji Y

Stabilization of microtubular cytoskeleton protects neurons from toxicity of N-terminal fragment of cytosolic prion protein.
Biochimica et biophysica acta1853(10 Pt A)2228-39(2015 Oct)
Zajkowski T,Nieznanska H,Nieznanski K

Primary blast-induced traumatic brain injury in rats leads to increased prion protein in plasma: a potential biomarker for blast-induced traumatic brain injury.
Journal of neurotrauma32(1)58-65(2015 Jan)
Pham N,Sawyer TW,Wang Y,Jazii FR,Vair C,Taghibiglou C

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Prnp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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