S100a4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	S100a4
Entrez Gene ID	24615
Full Name	S100 calcium-binding protein A4
Synonyms	18A2,42A,CAPL,MTS1,P9ka,PEL98,RNP9KA
General protein information	Preferred Names S100 calcium-binding protein A4 Names protein S100-A4 P9K Protein 9 Ka homologous to calcium-binding protein calvasculin metastasin nerve growth factor-induced protein 42A placental calcium-binding protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34
Summary	may play a role in regulation of cell growth and differentiation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012618.2	NP_036750.1	protein S100-A4
XM_006232592.3	XP_006232654.1	protein S100-A4 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	S100A4	NP_002952.1
	S100A4	XP_001110673.1
Danio rerio (zebrafish)	icn	NP_997926.1
Danio rerio (zebrafish)	icn2	NP_001076426.1
Mus musculus (house mouse)	S100a4	NP_035441.1
Gallus gallus (chicken)	S100A4	XP_004948276.1
Canis lupus familiaris (dog)	S100A4	NP_001003161.1
Bos taurus (cattle)	S100A4	NP_777020.1
Rattus norvegicus (Norway rat)	S100a4	NP_036750.1
Pan troglodytes (chimpanzee)	S100A4	XP_001138744.1

Gene Ontology
Bibliography

Related articles in PubMed

The microRNA miR-124 inhibits vascular smooth muscle cell proliferation by targeting S100 calcium-binding protein A4 (S100A4).
Choe N, Kwon DH, Shin S, Kim YS, Kim YK, Kim J, Ahn Y, Eom GH, Kook H
FEBS letters591(7)1041-1052(2017 04)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Involvement of fibroblast-specific protein 1 (S100A4) and matrix metalloproteinase-13 (MMP-13) in CCl4-induced reversible liver fibrosis.
Louka ML, Ramzy MM
Gene579(1)29-33(2016 Mar)

S100A4 upregulation suppresses tissue ossification and enhances matrix degradation in experimental periodontitis models.
Zhou M, Li ZQ, Wang ZL
Acta pharmacologica Sinica36(11)1388-94(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that microRNA miR-124 targets S100 calcium-binding protein A4 (S100A4) 3' UTR and regulates vascular smooth muscle cell proliferation.
Title: The microRNA miR-124 inhibits vascular smooth muscle cell proliferation by targeting S100 calcium-binding protein A4 (S100A4).

Results show S100A4 expression increasing during fibrotic stage and declining during resolution suggesting it as a good marker for liver fibrogenesis.
Title: Involvement of fibroblast-specific protein 1 (S100A4) and matrix metalloproteinase-13 (MMP-13) in CCl4-induced reversible liver fibrosis.

S100A4 is upregulated in the experimental rat periodontitis.
Title: S100A4 upregulation suppresses tissue ossification and enhances matrix degradation in experimental periodontitis models.

found a significant increase in the expression of S100A4 in vitreous samples of rats with diabetic retinopathy
Title: S100A4 is upregulated in proliferative diabetic retinopathy and correlates with markers of angiogenesis and fibrogenesis.

TG2 regulates S100A4-mediated mammary tumor cell migration.
Title: The role of TG2 in regulating S100A4-mediated mammary tumour cell migration.

The following S100a4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the S100a4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00477	NM_012618.2 Latest version!	Rattus norvegicus S100 calcium-binding protein A4 (S100a4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa00477	XM_006232592.3 Latest version!	Rattus norvegicus S100 calcium-binding protein A4 (S100a4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

S100a4 ( NM_012618.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036750.1 Rattus norvegicus S100 calcium-binding protein A4 (S100a4), mRNA.

Price & Availability↑

CloneID	ORa00477
Clone ID Related Accession (Same CDS sequence)	NM_012618.2 , XM_006232592.3
Accession Version	NM_012618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Rattus norvegicus(Norway rat)
Product	protein S100-A4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J03628.1. On Aug 31, 2012 this sequence version replaced NM_012618.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J03628.1, FQ215053.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGAGAC CCTTGGAGGA GGCCCTGGAT GTAATAGTGT CCACCTTCCA CAAATACTCA 
GGCAACGAGG GTGACAAGTT CAAGCTGAAC AAGACAGAGC TCAAGGAGCT ACTGACCAGG 
GAGCTGCCTA GCTTCCTGGG GAGAAGGACA GACGAAGCTG CATTCCAGAA GCTGATGAAC 
AACTTGGACA GCAACAGGGA CAATGAAGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCATTGCCA TGATGTGCAA TGAATTCTTT GAGGGCTGCC CAGATAAGGA GCCCCGGAAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036750.1
CDS	53..358
Translation	MARPLEEALDVIVSTFHKYSGNEGDKFKLNKTELKELLTRELPSFLGRRTDEAAFQKLMNNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGCPDKEPRKK

Target ORF information:

RefSeq Version	NM_012618.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus S100 calcium-binding protein A4 (S100a4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012618.2

ORF Insert Sequence:

ATGGCGAGAC CCTTGGAGGA GGCCCTGGAT GTAATAGTGT CCACCTTCCA CAAATACTCA 
GGCAACGAGG GTGACAAGTT CAAGCTGAAC AAGACAGAGC TCAAGGAGCT ACTGACCAGG 
GAGCTGCCTA GCTTCCTGGG GAGAAGGACA GACGAAGCTG CATTCCAGAA GCTGATGAAC 
AACTTGGACA GCAACAGGGA CAATGAAGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCATTGCCA TGATGTGCAA TGAATTCTTT GAGGGCTGCC CAGATAAGGA GCCCCGGAAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S100a4 ( XM_006232592.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232654.1 Rattus norvegicus S100 calcium-binding protein A4 (S100a4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa00477
Clone ID Related Accession (Same CDS sequence)	NM_012618.2 , XM_006232592.3
Accession Version	XM_006232592.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein S100-A4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232592.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAGAC CCTTGGAGGA GGCCCTGGAT GTAATAGTGT CCACCTTCCA CAAATACTCA 
GGCAACGAGG GTGACAAGTT CAAGCTGAAC AAGACAGAGC TCAAGGAGCT ACTGACCAGG 
GAGCTGCCTA GCTTCCTGGG GAGAAGGACA GACGAAGCTG CATTCCAGAA GCTGATGAAC 
AACTTGGACA GCAACAGGGA CAATGAAGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCATTGCCA TGATGTGCAA TGAATTCTTT GAGGGCTGCC CAGATAAGGA GCCCCGGAAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232654.1
CDS	126..431
Translation	MARPLEEALDVIVSTFHKYSGNEGDKFKLNKTELKELLTRELPSFLGRRTDEAAFQKLMNNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGCPDKEPRKK

Target ORF information:

RefSeq Version	XM_006232592.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus S100 calcium-binding protein A4 (S100a4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232592.3

ORF Insert Sequence:

ATGGCGAGAC CCTTGGAGGA GGCCCTGGAT GTAATAGTGT CCACCTTCCA CAAATACTCA 
GGCAACGAGG GTGACAAGTT CAAGCTGAAC AAGACAGAGC TCAAGGAGCT ACTGACCAGG 
GAGCTGCCTA GCTTCCTGGG GAGAAGGACA GACGAAGCTG CATTCCAGAA GCTGATGAAC 
AACTTGGACA GCAACAGGGA CAATGAAGTT GACTTCCAGG AGTACTGTGT CTTCCTGTCC 
TGCATTGCCA TGATGTGCAA TGAATTCTTT GAGGGCTGCC CAGATAAGGA GCCCCGGAAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The microRNA miR-124 inhibits vascular smooth muscle cell proliferation by targeting S100 calcium-binding protein A4 (S100A4).
FEBS letters591(7)1041-1052(2017 04)
Choe N,Kwon DH,Shin S,Kim YS,Kim YK,Kim J,Ahn Y,Eom GH,Kook H

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Involvement of fibroblast-specific protein 1 (S100A4) and matrix metalloproteinase-13 (MMP-13) in CCl4-induced reversible liver fibrosis.
Gene579(1)29-33(2016 Mar)
Louka ML,Ramzy MM

S100A4 upregulation suppresses tissue ossification and enhances matrix degradation in experimental periodontitis models.
Acta pharmacologica Sinica36(11)1388-94(2015 Nov)
Zhou M,Li ZQ,Wang ZL