S100g cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	S100g
Entrez Gene ID	24249
Full Name	S100 calcium binding protein G
Synonyms	Cabp,Calb3,Cbpi,Rncalbd9
General protein information	Preferred Names S100 calcium binding protein G Names protein S100-G 9 kDa CaBP calbindin 3, (vitamin D-dependent calcium binding protein) calbindin-D9K calcium-binding protein, intestinal, vitamin D-dependent (9-kDa CaBP) cholecalcin vitamin D-dependent calcium-binding protein, intestinal
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq14
Summary	calcium-binding protein that may be involved in intestinal calcium regulation; transcriptionally activated by 1,25-dihydroxyvitamin D3 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006256888.3	XP_006256950.1	protein S100-G isoform X1
NM_012521.2	NP_036653.1	protein S100-G

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	S100G	XP_849066.1
Rattus norvegicus (Norway rat)	S100g	NP_036653.1
Mus musculus (house mouse)	S100g	NP_033919.1
Homo sapiens (human)	S100G	NP_004048.1
Pan troglodytes (chimpanzee)	S100G	XP_001139782.1
	S100G	XP_001102900.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential effects of estrogen and estrogen receptor antagonist, ICI 182 780, on the expression of calbindin-D9k in rat pituitary prolactinoma GH? cells.
Wang W, Knosp E, Tai G, Zhao Y, Liang Q, Guo Y
International journal of clinical and experimental pathology7(12)8498-505(2014)

Participation of the oviductal s100 calcium binding protein G in the genomic effect of estradiol that accelerates oviductal embryo transport in mated rats.
R?os M, Parada-Bustamante A, Vel?squez LA, Croxatto HB, Orihuela PA
Reproductive biology and endocrinology : RB&E969(2011 May)

Cytosolic Ca2+ buffers.
Schwaller B
Cold Spring Harbor perspectives in biology2(11)a004051(2010 Nov)

Estrogen regulates the localization and expression of calbindin-D9k in the pituitary gland of immature male rats via the ERalpha-pathway.
Tinnanooru P, Dang VH, Nguyen TH, Lee GS, Choi KC, Jeung EB
Molecular and cellular endocrinology285(1-2)26-33(2008 Mar)

Effects of calcitonin and parathyroid hormone on the regulation of cabindin-D(9k) in the uterus, placenta, and fetal membrane of rats related to blood calcium level during late gestation.
Hong EJ, Choi KC, Hyun SH, Jeung EB
Molecular reproduction and development74(9)1188-97(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Estrogen-induced CaBP-9k directly combines with ERalpha, suggesting that CaBP-9k is involved in the biological effects mediated by an ER pathway in the GH3 rat pituitary prolactinoma cell line
Title: Differential effects of estrogen and estrogen receptor antagonist, ICI 182 780, on the expression of calbindin-D9k in rat pituitary prolactinoma GH? cells.

S100 g is a functional component of E2 genomic effect that accelerates egg transport.
Title: Participation of the oviductal s100 calcium binding protein G in the genomic effect of estradiol that accelerates oviductal embryo transport in mated rats.

Results suggest that pituitary calbindin-D9k is induced by estrogen in male rats and its expression is predominantly regulated by estrogen receptor alpha, but not ERbeta.
Title: Estrogen regulates the localization and expression of calbindin-D9k in the pituitary gland of immature male rats via the ERalpha-pathway.

There was an increase in the level of CaBP-9k in the uterus, placenta, and extra-embryonic membrane at late gestation, as blood calcium level increased.
Title: Effects of calcitonin and parathyroid hormone on the regulation of cabindin-D(9k) in the uterus, placenta, and fetal membrane of rats related to blood calcium level during late gestation.

Pituitary CaBP-9k expression is regulated during estrous cycle.Expression is mainly controlled by E2 & antagonized by P4. Pituitary CaBP-9k in female rats may be involved in the central function of the reproduction system.
Title: A calcium binding protein, calbindin-D9k, is mainly regulated by estrogen in the pituitary gland of rats during estrous cycle.

The following S100g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the S100g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00183	NM_012521.2 Latest version!	Rattus norvegicus S100 calcium binding protein G (S100g), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa39697	XM_006256888.3 Latest version!	Rattus norvegicus S100 calcium binding protein G (S100g), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

S100g ( NM_012521.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036653.1 Rattus norvegicus S100 calcium binding protein G (S100g), mRNA.

Price & Availability↑

CloneID	ORa00183
Clone ID Related Accession (Same CDS sequence)	NM_012521.2
Accession Version	NM_012521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	protein S100-G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J02954.1. On Aug 30, 2012 this sequence version replaced NM_012521.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J02954.1, BC059153.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGCTA AGAAATCTCC CGAAGAAATG AAGAGCATTT TTCAAAAATA TGCAGCCAAA 
GAAGGCGATC CAAACCAGCT GTCCAAGGAG GAGCTGAAGC TGCTGATTCA GTCAGAGTTC 
CCCAGCCTCC TGAAGGCTTC AAGTACTCTA GACAATCTCT TTAAAGAGCT GGATAAGAAC 
GGTGATGGAG AAGTTAGCTA TGAAGAATTC GAAGTTTTCT TCAAAAAGTT ATCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036653.1
CDS	62..301
Translation	MSAKKSPEEMKSIFQKYAAKEGDPNQLSKEELKLLIQSEFPSLLKASSTLDNLFKELDKNGDGEVSYEEFEVFFKKLSQ

Target ORF information:

RefSeq Version	NM_012521.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus S100 calcium binding protein G (S100g), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012521.2

ORF Insert Sequence:

ATGAGCGCTA AGAAATCTCC CGAAGAAATG AAGAGCATTT TTCAAAAATA TGCAGCCAAA 
GAAGGCGATC CAAACCAGCT GTCCAAGGAG GAGCTGAAGC TGCTGATTCA GTCAGAGTTC 
CCCAGCCTCC TGAAGGCTTC AAGTACTCTA GACAATCTCT TTAAAGAGCT GGATAAGAAC 
GGTGATGGAG AAGTTAGCTA TGAAGAATTC GAAGTTTTCT TCAAAAAGTT ATCACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

S100g ( XM_006256888.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256950.1 Rattus norvegicus S100 calcium binding protein G (S100g), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa39697
Clone ID Related Accession (Same CDS sequence)	XM_006256888.3
Accession Version	XM_006256888.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein S100-G isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256888.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCTA AGAAATCTCC CGAAGAAATG AAGAGCATTT TTCAAAAATA TGCAGCCAAA 
GAAGGCGATC CAAACCAGCT GTCCAAGGAG GAGCTGAAGC TGCTGATTCA GTCAGAGTTC 
CCCAGCCTCC TGAAGCAGGC TTCAAGTACT CTAGACAATC TCTTTAAAGA GCTGGATAAG 
AACGGTGATG GAGAAGTTAG CTATGAAGAA TTCGAAGTTT TCTTCAAAAA GTTATCACAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256950.1
CDS	428..670
Translation	MSAKKSPEEMKSIFQKYAAKEGDPNQLSKEELKLLIQSEFPSLLKQASSTLDNLFKELDKNGDGEVSYEEFEVFFKKLSQ

Target ORF information:

RefSeq Version	XM_006256888.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus S100 calcium binding protein G (S100g), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256888.3

ORF Insert Sequence:

ATGAGCGCTA AGAAATCTCC CGAAGAAATG AAGAGCATTT TTCAAAAATA TGCAGCCAAA 
GAAGGCGATC CAAACCAGCT GTCCAAGGAG GAGCTGAAGC TGCTGATTCA GTCAGAGTTC 
CCCAGCCTCC TGAAGCAGGC TTCAAGTACT CTAGACAATC TCTTTAAAGA GCTGGATAAG 
AACGGTGATG GAGAAGTTAG CTATGAAGAA TTCGAAGTTT TCTTCAAAAA GTTATCACAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential effects of estrogen and estrogen receptor antagonist, ICI 182 780, on the expression of calbindin-D9k in rat pituitary prolactinoma GH? cells.
International journal of clinical and experimental pathology7(12)8498-505(2014)
Wang W,Knosp E,Tai G,Zhao Y,Liang Q,Guo Y

Participation of the oviductal s100 calcium binding protein G in the genomic effect of estradiol that accelerates oviductal embryo transport in mated rats.
Reproductive biology and endocrinology : RB&E969(2011 May)
R?os M,Parada-Bustamante A,Vel?squez LA,Croxatto HB,Orihuela PA

Cytosolic Ca2+ buffers.
Cold Spring Harbor perspectives in biology2(11)a004051(2010 Nov)
Schwaller B

Estrogen regulates the localization and expression of calbindin-D9k in the pituitary gland of immature male rats via the ERalpha-pathway.
Molecular and cellular endocrinology285(1-2)26-33(2008 Mar)
Tinnanooru P,Dang VH,Nguyen TH,Lee GS,Choi KC,Jeung EB

Effects of calcitonin and parathyroid hormone on the regulation of cabindin-D(9k) in the uterus, placenta, and fetal membrane of rats related to blood calcium level during late gestation.
Molecular reproduction and development74(9)1188-97(2007 Sep)
Hong EJ,Choi KC,Hyun SH,Jeung EB