Nos1ap cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nos1ap
Entrez Gene ID	192363
Full Name	nitric oxide synthase 1 adaptor protein
Synonyms	Capon
General protein information	Preferred Names nitric oxide synthase 1 adaptor protein Names carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein C-terminal PDZ domain ligand of neuronal nitric oxide synthase C-terminal PDZ ligand of neuronal nitric oxide synthase protein nitric oxide synthase 1 (neuronal) adaptor protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q24
Summary	involved in regulating the interaction between neuronal nitric oxide synthase and PSD95 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138922.1	NP_620277.1	carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein

Pathways from BioSystems

Homologies

	NOS1AP	NP_001158236.1
Drosophila melanogaster (fruit fly)	CG42673	NP_001189078.1
Mus musculus (house mouse)	Nos1ap	NP_001103455.1
Rattus norvegicus (Norway rat)	Nos1ap	NP_620277.1
Danio rerio (zebrafish)	nos1apa	XP_002662526.3

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of Isoforms of Nitric Oxide Synthase 1 Adaptor Protein, Encoded by a Risk Gene for Schizophrenia, Alters Actin Dynamics and Synaptic Function.
Hernandez K, Swiatkowski P, Patel MV, Liang C, Dudzinski NR, Brzustowicz LM, Firestein BL
Frontiers in cellular neuroscience106(2016)

NOS1AP Functionally Associates with YAP To Regulate Hippo Signaling.
Clattenburg L, Wigerius M, Qi J, Rainey JK, Rourke JL, Muruganandan S, Sinal CJ, Fawcett JP
Molecular and cellular biology35(13)2265-77(2015 Jul)

Nitric oxide synthase 1 adaptor protein, a protein implicated in schizophrenia, controls radial migration of cortical neurons.
Carrel D, Hernandez K, Kwon M, Mau C, Trivedi MP, Brzustowicz LM, Firestein BL
Biological psychiatry77(11)969-78(2015 Jun)

CAPON modulates neuronal calcium handling and cardiac sympathetic neurotransmission during dysautonomia in hypertension.
Lu CJ, Hao G, Nikiforova N, Larsen HE, Liu K, Crabtree MJ, Li D, Herring N, Paterson DJ
Hypertension (Dallas, Tex. : 1979)65(6)1288-1297(2015 Jun)

NOS1AP modulates intracellular Ca(2+) in cardiac myocytes and is up-regulated in dystrophic cardiomyopathy.
Treuer AV, Gonzalez DR
International journal of physiology, pathophysiology and pharmacology6(1)37-46(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study showed that critical role for the schizophrenia-associated gene NOS1AP in cortical patterning and neuron migration.
Title: Nitric oxide synthase 1 adaptor protein, a protein implicated in schizophrenia, controls radial migration of cortical neurons.

CAPON modulates neuronal calcium during dysautonomia in hypertension.
Title: CAPON modulates neuronal calcium handling and cardiac sympathetic neurotransmission during dysautonomia in hypertension.

nNOS and CAPON co-localize in the pre-sympathetic autonomic neurons in the paraventricular nucleus and their expression is reciprocally regulated in congestive heart failure via angiotensin II.
Title: Decreased nNOS in the PVN leads to increased sympathoexcitation in chronic heart failure: role for CAPON and Ang II.

CAPON might play a proactive role in the process of inflammation by transferring from cytoplasm to the nucleus and through the NMDA-nNOS signal pathway
Title: The nuclear localization of CAPON in hippocampus and cerebral cortex neurons after lipopolysaccharide stimulation.

NOS1AP plays an important role in the mammalian synapse.
Title: NOS1AP associates with Scribble and regulates dendritic spine development.

The following Nos1ap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nos1ap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02044	NM_138922.1 Latest version!	Rattus norvegicus nitric oxide synthase 1 adaptor protein (Nos1ap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$293.30 $419.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nos1ap ( NM_138922.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620277.1 Rattus norvegicus nitric oxide synthase 1 adaptor protein (Nos1ap), mRNA.

Price & Availability↑

CloneID	ORa02044
Clone ID Related Accession (Same CDS sequence)	NM_138922.1
Accession Version	NM_138922.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF037071.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF037071.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCCAGCA AAACCAAGTA CAACCTTGTG GACGATGGGC ACGACTTACG GATCCCTTTG 
CACAACGAGG ACGCCTTCCA GCACGGCATC TCTTTTGAGG CCAAGTACGT GGGAAGCCTG 
GATGTGCCCA GACCCAACAG CAGGGTTGAG ATCGTGGCTG CCATGCGCAG AATCCGGTAT 
GAGTTTAAAG CCAAGAATAT CAAGAAGAAG AAAGTAAGCA TCATGGTCTC CGTGGACGGT 
GTCAAAGTGA TTCTGAAGAA GAAGAAAAAG AAAAAGGAGT GGACGTGGGA TGAGAGCAAG 
ATGCTGGTGA TGCAGGACCC TATCTACAGG ATCTTCTATG TCTCTCATGA CTCCCAAGAC 
TTGAAAATCT TCAGCTATAT CGCTCGAGAT GGTGCCAGCA ATATCTTCAG ATGCAATGTC 
TTTAAATCCA AGAAGAAGAG CCAAGCTATG AGAATCGTAC GGACAGTGGG ACAGGCCTTT 
GAGGTCTGCC ACAAGCTGAG CCTGCAGCAC ACACAGCAGA ATGCAGATGG CCAGGAAGAT 
GGAGAGAGCG AGAGGAACAG CGATGGCTCA GGAGACCCAG GCCGCCAGCT CACTGGAGCT 
GAGAGGGTCT CCACAGCCAC CGCAGAGGAG ACCGACATTG ACGCTGTGGA GGTCCCACTC 
CCCGGGAATG ACATTCTAGA ATTCAGCCGA GGTGTGACTG ACCTGGATGC TATTGGGAAG 
GACGGAGGCT CCCACATAGA CACGACGGTC TCACCCCATC CACAGGAGCC CATGCTGGCA 
GCCTCCCCTC GCATGCTGCT CCCTTCTTCT TCTTCCTCGA AGCCACCGGG CTTGGGCACT 
GGGACGCCCC TGTCCACTCA CCACCAGATG CAGCTCCTCC AGCAGCTCCT CCAGCAGCAG 
CAGCAGCAGA CACAAGTGGC TGTGGCTCAG GTTCACTTGC TGAAGGATCA GTTGGCTGCT 
GAGGCTGCGG CACGGCTGGA GGCCCAGGCA CGAGTGCACC AGCTCCTGCT ACAGAACAAA 
GACATGCTTC AGCACATCTC TCTGCTGGTT AAGCAGGTGC AGGAGCTGGA ACTGAAGCTG 
TCAGGACAGA GCACCATGGG CTCCCAGGAC AGCTTGCTGG AGATCACCTT CCGTTCAGGT 
GCCCTGCCTG TGCTCTGTGA ATCCACCACT CCTAAGCCAG AGGACCTACA CTCACCACTG 
CTGGGCGCTG GCTTGGCTGA CTTTGCCCAC CCAGTGGGCA GCCCCTTAGG TAGGCGTGAC 
TGCTTGGTGA AGCTGGAGTG CTTTCGTTTC CTCCCAGCCG AGGATAACCA GCCGATGGCA 
CAGGGTGAGC CGCTCCTAGG TGGCCTGGAG CTCATCAAGT TCCGAGAGTC AGGCATCGCC 
TCAGAGTATG AGTCCAACAC AGACGAAAGC GAGGAGCGTG ACTCGTGGTC GCAGGAAGAG 
CTGCCACGCC TGCTCAATGT CCTACAGCGG CAGGAGTTGG GTGACAGTTT GGATGATGAG 
ATCGCCGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620277.1
CDS	393..1904
Translation	MPSKTKYNLVDDGHDLRIPLHNEDAFQHGISFEAKYVGSLDVPRPNSRVEIVAAMRRIRYEFKAKNIKKKKVSIMVSVDGVKVILKKKKKKKEWTWDESKMLVMQDPIYRIFYVSHDSQDLKIFSYIARDGASNIFRCNVFKSKKKSQAMRIVRTVGQAFEVCHKLSLQHTQQNADGQEDGESERNSDGSGDPGRQLTGAERVSTATAEETDIDAVEVPLPGNDILEFSRGVTDLDAIGKDGGSHIDTTVSPHPQEPMLAASPRMLLPSSSSSKPPGLGTGTPLSTHHQMQLLQQLLQQQQQQTQVAVAQVHLLKDQLAAEAAARLEAQARVHQLLLQNKDMLQHISLLVKQVQELELKLSGQSTMGSQDSLLEITFRSGALPVLCESTTPKPEDLHSPLLGAGLADFAHPVGSPLGRRDCLVKLECFRFLPAEDNQPMAQGEPLLGGLELIKFRESGIASEYESNTDESEERDSWSQEELPRLLNVLQRQELGDSLDDEIAV

Target ORF information:

RefSeq Version	NM_138922.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nitric oxide synthase 1 adaptor protein (Nos1ap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138922.1

ORF Insert Sequence:

ATGCCCAGCA AAACCAAGTA CAACCTTGTG GACGATGGGC ACGACTTACG GATCCCTTTG 
CACAACGAGG ACGCCTTCCA GCACGGCATC TCTTTTGAGG CCAAGTACGT GGGAAGCCTG 
GATGTGCCCA GACCCAACAG CAGGGTTGAG ATCGTGGCTG CCATGCGCAG AATCCGGTAT 
GAGTTTAAAG CCAAGAATAT CAAGAAGAAG AAAGTAAGCA TCATGGTCTC CGTGGACGGT 
GTCAAAGTGA TTCTGAAGAA GAAGAAAAAG AAAAAGGAGT GGACGTGGGA TGAGAGCAAG 
ATGCTGGTGA TGCAGGACCC TATCTACAGG ATCTTCTATG TCTCTCATGA CTCCCAAGAC 
TTGAAAATCT TCAGCTATAT CGCTCGAGAT GGTGCCAGCA ATATCTTCAG ATGCAATGTC 
TTTAAATCCA AGAAGAAGAG CCAAGCTATG AGAATCGTAC GGACAGTGGG ACAGGCCTTT 
GAGGTCTGCC ACAAGCTGAG CCTGCAGCAC ACACAGCAGA ATGCAGATGG CCAGGAAGAT 
GGAGAGAGCG AGAGGAACAG CGATGGCTCA GGAGACCCAG GCCGCCAGCT CACTGGAGCT 
GAGAGGGTCT CCACAGCCAC CGCAGAGGAG ACCGACATTG ACGCTGTGGA GGTCCCACTC 
CCCGGGAATG ACATTCTAGA ATTCAGCCGA GGTGTGACTG ACCTGGATGC TATTGGGAAG 
GACGGAGGCT CCCACATAGA CACGACGGTC TCACCCCATC CACAGGAGCC CATGCTGGCA 
GCCTCCCCTC GCATGCTGCT CCCTTCTTCT TCTTCCTCGA AGCCACCGGG CTTGGGCACT 
GGGACGCCCC TGTCCACTCA CCACCAGATG CAGCTCCTCC AGCAGCTCCT CCAGCAGCAG 
CAGCAGCAGA CACAAGTGGC TGTGGCTCAG GTTCACTTGC TGAAGGATCA GTTGGCTGCT 
GAGGCTGCGG CACGGCTGGA GGCCCAGGCA CGAGTGCACC AGCTCCTGCT ACAGAACAAA 
GACATGCTTC AGCACATCTC TCTGCTGGTT AAGCAGGTGC AGGAGCTGGA ACTGAAGCTG 
TCAGGACAGA GCACCATGGG CTCCCAGGAC AGCTTGCTGG AGATCACCTT CCGTTCAGGT 
GCCCTGCCTG TGCTCTGTGA ATCCACCACT CCTAAGCCAG AGGACCTACA CTCACCACTG 
CTGGGCGCTG GCTTGGCTGA CTTTGCCCAC CCAGTGGGCA GCCCCTTAGG TAGGCGTGAC 
TGCTTGGTGA AGCTGGAGTG CTTTCGTTTC CTCCCAGCCG AGGATAACCA GCCGATGGCA 
CAGGGTGAGC CGCTCCTAGG TGGCCTGGAG CTCATCAAGT TCCGAGAGTC AGGCATCGCC 
TCAGAGTATG AGTCCAACAC AGACGAAAGC GAGGAGCGTG ACTCGTGGTC GCAGGAAGAG 
CTGCCACGCC TGCTCAATGT CCTACAGCGG CAGGAGTTGG GTGACAGTTT GGATGATGAG 
ATCGCCGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression of Isoforms of Nitric Oxide Synthase 1 Adaptor Protein, Encoded by a Risk Gene for Schizophrenia, Alters Actin Dynamics and Synaptic Function.
Frontiers in cellular neuroscience106(2016)
Hernandez K,Swiatkowski P,Patel MV,Liang C,Dudzinski NR,Brzustowicz LM,Firestein BL

NOS1AP Functionally Associates with YAP To Regulate Hippo Signaling.
Molecular and cellular biology35(13)2265-77(2015 Jul)
Clattenburg L,Wigerius M,Qi J,Rainey JK,Rourke JL,Muruganandan S,Sinal CJ,Fawcett JP

Nitric oxide synthase 1 adaptor protein, a protein implicated in schizophrenia, controls radial migration of cortical neurons.
Biological psychiatry77(11)969-78(2015 Jun)
Carrel D,Hernandez K,Kwon M,Mau C,Trivedi MP,Brzustowicz LM,Firestein BL

CAPON modulates neuronal calcium handling and cardiac sympathetic neurotransmission during dysautonomia in hypertension.
Hypertension (Dallas, Tex. : 1979)65(6)1288-1297(2015 Jun)
Lu CJ,Hao G,Nikiforova N,Larsen HE,Liu K,Crabtree MJ,Li D,Herring N,Paterson DJ

NOS1AP modulates intracellular Ca(2+) in cardiac myocytes and is up-regulated in dystrophic cardiomyopathy.
International journal of physiology, pathophysiology and pharmacology6(1)37-46(2014)
Treuer AV,Gonzalez DR