Plb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plb1
Entrez Gene ID	192259
Full Name	phospholipase B1
Synonyms	Phlpb
General protein information	Preferred Names phospholipase B1 Names phospholipase B1, membrane-associated PLB/LIP phospholipase B phospholipase B/lipase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q14
Summary	exhibits broad lipolytic activities (PLA2, lysophospholipase, and lipase activities); may mediate terminal digestion, or membrane digestion, of dietary lipids and biliary phospholipids [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138898.1	NP_620253.1	phospholipase B1, membrane-associated precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PLB1	XP_005630350.1
Mus musculus (house mouse)	Plb1	NP_001074876.1
Rattus norvegicus (Norway rat)	Plb1	NP_620253.1
Homo sapiens (human)	PLB1	NP_694566.4
Gallus gallus (chicken)	PLB1	XP_004935430.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011512	XP_315274.4
Xenopus tropicalis (tropical clawed frog)	LOC100495859	XP_004914985.1
Pan troglodytes (chimpanzee)	PLB1	XP_515372.2
Xenopus tropicalis (tropical clawed frog)	LOC100492698	XP_002943699.2
Bos taurus (cattle)	PLB1	XP_002691542.2
Danio rerio (zebrafish)	si:ch211-214p16.3	XP_689138.6
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011513	XP_315272.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011511	XP_315275.3

Gene Ontology
Bibliography

Related articles in PubMed

Phospholipase B is activated in response to sterol removal and stimulates acrosome exocytosis in murine sperm.
Asano A, Nelson-Harrington JL, Travis AJ
The Journal of biological chemistry288(39)28104-15(2013 Sep)

Mechanisms involved in the intestinal absorption of dietary vitamin A and provitamin A carotenoids.
Harrison EH
Biochimica et biophysica acta1821(1)70-7(2012 Jan)

Identification of functional domains of rat intestinal phospholipase B/lipase. Its cDNA cloning, expression, and tissue distribution.
Takemori H, Zolotaryov FN, Ting L, Urbain T, Komatsubara T, Hatano O, Okamoto M, Tojo H
The Journal of biological chemistry273(4)2222-31(1998 Jan)

Purification and partial characterization of a retinyl ester hydrolase from the brush border of rat small intestine mucosa: probable identity with brush border phospholipase B.
Rigtrup KM, Kakkad B, Ong DE
Biochemistry33(9)2661-6(1994 Mar)

Further characterization of a novel phospholipase B (phospholipase A2--lysophospholipase) from intestinal brush-border membranes.
Pind S, Kuksis A
Biochemistry and cell biology = Biochimie et biologie cellulaire69(5-6)346-57(1991 May-Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Plb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01932	NM_138898.1 Latest version!	Rattus norvegicus phospholipase B1 (Plb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1063.30 $1519.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plb1 ( NM_138898.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620253.1 Rattus norvegicus phospholipase B1 (Plb1), mRNA.

Price & Availability↑

CloneID	ORa01932
Clone ID Related Accession (Same CDS sequence)	NM_138898.1
Accession Version	NM_138898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Rattus norvegicus(Norway rat)
Product	phospholipase B1, membrane-associated precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D63648.1. ##Evidence-Data-START## Transcript exon combination :: D63648.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGTCAT GGCCAGGCGT TTCCCTGGTG GGACTGCTGC TGCTGCTACT GCTGGGACAA 
GGGCCCTCCC AAATCCACGG CTCTTCTGGA GAAAACACAT CGCAGCCCCA GCAAGTGTTT 
CGGACCCTGA AGAATTTTTC ATTCCCTTGC AAGCCAAAGA AGTTAGAACT GAGTGTGCTT 
TCTAAGTCAG TTCACTCTCT GAGACCCTCA GACATTAAAC TCGTGGCAGC CATCGGCAAC 
CTAGAAACTC CTCCAGCCCC TGGCTCAGGC GTGGTCAACA TGGAGAAACC TCAAAGCCTC 
GAGAGCGAAC TACAGAATGT GTGCATAGGA ATCATGACAG CCCTTTCAGA TATCATCAGA 
CATTTCAACC CTTCTGTTCT GATGCCCACG TGTTCTCCTG GGAAGGGTAC TGCAGGCCAC 
ACTACTATTG CCGAAGACTT GTGGATTCAG GCTAAAGAGC TGGTGAGGCA TCTGAAAGAC 
AACCCGGAAC TTGATTTTGA GAAGGACTGG AAACTCATCA CTGTGCTCTT CAGTAACACA 
AGCCAGTGCC ACCTGTGTTC CTCTGATCAG CAGAAAAGGC ACTTGATGAA GCACATGGAG 
ATGCTGTCAG GGGTGCTGGA TTACCTGCAT CGTGAGGTCC CCAGAGCGTT TGTGAATTTG 
GTGGATCTCT CTGAGGTTTT GACCATGGCT CAGCAGCATC AAGAGACTGG TTTCAGCCCT 
GCACCAGAGA TTTGCAAATG CTCAGAGGAA ATAACGAAGT TATCCAAGGC TGTCATGCAG 
TGGTCCTATC AGGAAGCCTG GGAAGATCTC CTGGCCTCCA GCAAGTTCAA TAAGCATGAG 
ACCTTCGCTG TGGTTTTCCA GTCTTTCTTC TCTGAGGTAG AACTACCCTT GGAGAGGCCC 
TCGCCCCAGG ATTCCACCAC GCTCGCCCTC AGGATCTGGA ATAGCATGAT GGAACCAGTG 
GGTCGAAAGG ATGGGACACT CAATGAAGCA GAGAGAAAAA CAATGAAATG TCCCTCTCAG 
GAGAGCCCCT ATCTGTTCAC CTACAGAAAT AGCAACTACC AGGCCAGACA GCTGAAACCC 
ATAGGAAAGT TTCAGATGAA AGAAGGAACA AAATTCACCT GTCCTGACAA AGACCCCTCG 
GACTCCATCC CCACAACAGT TCACAGGCTG AGGCCGGCTG ACATCAAGGT CATCGGAGCC 
ATGGGTGACT CGCTCACGGC AGGCAACGGG GCAGGGTCCA GCCCTGGGAA TGTCTTGGAT 
GTCTTAACTC AATACCGAGG CCTGTCGTGG AGTGTGGGCG GAGATGAGAC CATCGAGACC 
GTGACCACCC TAGCCAACAT CCTCCGGGAA TTCAACCCCT CCTTGAAGGG CTTCTCTGTT 
GGCACTGGGA AAGAAAACAC TCCCCGAGCA TCCTTCAACC AGGCCGTAGC AGGAGCCAAA 
TCTGATGGCT TAGCTGCCCA GGCCAAAAAG CTGGTGAGCC TGATGAAGGA TGACAAGACA 
ATAAACTTTC AGGAAGACTG GAAGATAATC ACTGTGTTTA TAGGAGGCAA CGACCTCTGT 
GGCTCCTGCA ATAACCTGGC TCGCTTTTCT CCCCAAACCT TCACAGACAA CATCAAGACG 
GCCCTGGACA TCCTCCATGC AGAGGTTCCC CGGGCCTTTG TGAACATGGT CTCGGTGATT 
GAGATCACCC CCTTGAGAGA ACTGTTCAAT GAACCTAAAG TCAGCTGCCC ACGGATGATC 
CTCAGGAGCC TGTGTCCTTG TGTCTTGAAC CTTGGTGAGA ACTCAGCAGA ACTTGCCCAA 
CTTGTGGAAA GGAACAGGCA GTATCAGGAA GAAACTGGAA AACTGATTGA GAGTGGCCGA 
TACGACACCA GGGATGACTT CACCGTGGTC CTCCAGCCCA TGTTTGAAAA TGTCGTCATG 
CCACGGACCC TGGAGGGCTT GCCCGACAGC TCTTTCTTTG CCCCTGACTG TTTCCACTTC 
AATGTCAAGA CTCACGCTCG CTCAGCCATC GCCCTCTGGA AGAACATGCT AGAACCTGTG 
GGCCGCAAGA CAAGACATCA GAATTTTGAA ATCAAGGTCC CTATCATGTG TCCAAACCAG 
ACCTCACCGT TTCTGAGCAC CACCAAGAAC AGCAACCTGG GACATGGAAC TTCGATGTCT 
TGTGAGGAGA AAGCCCCCTC TGCCTCACCA CCAACCTCAG TGCATACCCT GAGACCTGCA 
GACATCCAAG TTGTGGCAGC TCTGGGAGAC TCTGTGACTG CTGGCAATGG AATCAGCTCC 
CAAGAAGGTG ATCTCGCTGA TGTTACCACA CAGTATCGAG GACTGTCCTA CAGTGCTGGT 
GGGGACAAGT TCCTGGAGAA TGTGACCACC TTGCCCAACA TCCTCCGGGA ATTTAATGGA 
AATCTCACAG GCTACTCAGT CGGAACCGGT GACGTCAACT CTGCAAGCGC GTTCCTTAAC 
CAGGCTGTCC CTGGGGCAAA GGCTGAGAAC CTTGCAAGTC AAGTCCAGAC TCTGATTCAG 
AAGATGAAGA ATGACACCAG AGTGAACTTT CACCAAGACT GGAAGGTCAT CACTGTGATG 
ATTGGGGCCA GCGACTTGTG TGACTTCTGC AAGGATTCGA ATCGTTACTC TGCAGCCAAT 
TTTTCTGACC ATCTCCGCAA TGCCTTGGAC ATCCTGCATA AGGAGGTACC CAGAGCCCTG 
GTCAACCTTG TGGACTTCAT GAACCCCAGT ATCATTCGGC AAGTGTTCCT GAAGAACCCA 
GACAAGTGCC CTGTGAATCA GACCAGTGTC CTGTGCAACT GTGTTCTGAC CCCAGGGGAG 
GATTCCCATG AGCTGGCAAG GTTGGAGGCC TTCACCAAAT CCTACCAGAG TAGCATGCTT 
CAACTGGTTG AGTCAGGCCG CTACGACACC CGGGAGGATT TCTCTGTGGT ACTGCAGCCC 
TTTCTCTTCA ACATCAGGCT CCCCATCCTA GAGAATGGGA ATCCAGATAC ATCCTTCTTT 
GCCCCAGACT GCATCCTCCT AAGCCAGAAG TTCCACACTC AGCTCGCGAG AGCCCTTTGG 
GCCAATATGC TTGAACCCCT GGGAAAGAAA ATGGATACTT TGGACCCGAA AGAACTCATA 
GCTTTGGCCT GCCCCACCAA GGACAAGCCC TTCCTGAGAA CCTTCCGGAA CAGTAACTAC 
ACGTACCCTA TCAAGCCAGC CATTGAGAAT TGGGGCAGTG ACTTCCTGTG CACAGAGCAG 
AGTCCTTCCA GCAAGGTACC CACCTCAGTT CATGAGCTCC GACCATCAGA CATCAAGGTG 
GTGGCAGCAA TGGGTGACTT TCTGACGACA GCCACCGGAG CTCGACCAAG TGAGTCCAGC 
AGTCTAGACA CCCCCTGGAG GGGGCTGTCT TGGAGCATTG GAGGAGATGG AACGTTGGAG 
ACCCATACCA CACTGCCCAA CATCCTGAAG AAGTTCAACC CTTCCATCCT TGGATTCTCC 
ACCGGTACCC TGGAGAACAC GGCAGGATTA AATGTGGCAG AAGAAGGCGC CAGAGCTCAG 
GACATGCCGG CCCAGGCTCA GGCCCTGGTG AAGAAGATGA AAAGCACCCC TACAATCAAC 
ATACAGGAAG ACTGGAAGCT GATTACACTC CTCATCGGGA ACAACGACCT GTGTCTTTAC 
TGTGAGGATC CGGAGAACTA CTCAACCAGG GAGTATGTCA AGTACATCCA GCATGCCTTG 
GACATCTTCT ATGAGGAGCT TCCCAGGGTT TTCATCAACG TGGTGGAAGT CATGGAGCTG 
TCCGGTCTGC TCCACGACCA GGGCGGGAAA TGTGCCATGC CGTTGGCTGT CCAGAAAAAC 
TGCAGTTGCC TTAAACGCTC TCAAAACCTC ATGGCAATGC AGGAGCTGAA GAAAGTCAAC 
GGGAACCTCC AGAGTGCCCT CTCGGAGCTC TCCTACTGGC ATCGGTACAT GCAGCGTGAG 
GACTTTGCAG TCACTGTCCA GCCTTTCTTC CGGAATACCT TTGTCCCACT GGATGAGCGT 
GGGGGCCTCG ACCTCACTTT CTTCTCTGAA GACTGTTTCC ACTTCTCAGT CCGTGGGCAT 
GCTGAGATGG CCATTGCCCT CTGGAACAAC ATGCTGGAAC CAGTGGGCAA GAAGACAACC 
TCCAATAACT TCACATACAA CCGGACCAAA CTCAAGTGTC CCTCGCCTGA AAACCCTTTC 
CTCTACACTG TCCGGAACAG TCAGATTCTT CTAGACAAGG CTAAAGAAAA CTCCAATACA 
CTCTACTGGG CAGTGCCAGT GGCTGCAGTA GGTGGCCTGG TAGTTGGCAT CCTTGGAATG 
ATGTTGTGGA GAACTGTGAG ACTCGTCCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620253.1
CDS	36..4388
Translation	MESWPGVSLVGLLLLLLLGQGPSQIHGSSGENTSQPQQVFRTLKNFSFPCKPKKLELSVLSKSVHSLRPSDIKLVAAIGNLETPPAPGSGVVNMEKPQSLESELQNVCIGIMTALSDIIRHFNPSVLMPTCSPGKGTAGHTTIAEDLWIQAKELVRHLKDNPELDFEKDWKLITVLFSNTSQCHLCSSDQQKRHLMKHMEMLSGVLDYLHREVPRAFVNLVDLSEVLTMAQQHQETGFSPAPEICKCSEEITKLSKAVMQWSYQEAWEDLLASSKFNKHETFAVVFQSFFSEVELPLERPSPQDSTTLALRIWNSMMEPVGRKDGTLNEAERKTMKCPSQESPYLFTYRNSNYQARQLKPIGKFQMKEGTKFTCPDKDPSDSIPTTVHRLRPADIKVIGAMGDSLTAGNGAGSSPGNVLDVLTQYRGLSWSVGGDETIETVTTLANILREFNPSLKGFSVGTGKENTPRASFNQAVAGAKSDGLAAQAKKLVSLMKDDKTINFQEDWKIITVFIGGNDLCGSCNNLARFSPQTFTDNIKTALDILHAEVPRAFVNMVSVIEITPLRELFNEPKVSCPRMILRSLCPCVLNLGENSAELAQLVERNRQYQEETGKLIESGRYDTRDDFTVVLQPMFENVVMPRTLEGLPDSSFFAPDCFHFNVKTHARSAIALWKNMLEPVGRKTRHQNFEIKVPIMCPNQTSPFLSTTKNSNLGHGTSMSCEEKAPSASPPTSVHTLRPADIQVVAALGDSVTAGNGISSQEGDLADVTTQYRGLSYSAGGDKFLENVTTLPNILREFNGNLTGYSVGTGDVNSASAFLNQAVPGAKAENLASQVQTLIQKMKNDTRVNFHQDWKVITVMIGASDLCDFCKDSNRYSAANFSDHLRNALDILHKEVPRALVNLVDFMNPSIIRQVFLKNPDKCPVNQTSVLCNCVLTPGEDSHELARLEAFTKSYQSSMLQLVESGRYDTREDFSVVLQPFLFNIRLPILENGNPDTSFFAPDCILLSQKFHTQLARALWANMLEPLGKKMDTLDPKELIALACPTKDKPFLRTFRNSNYTYPIKPAIENWGSDFLCTEQSPSSKVPTSVHELRPSDIKVVAAMGDFLTTATGARPSESSSLDTPWRGLSWSIGGDGTLETHTTLPNILKKFNPSILGFSTGTLENTAGLNVAEEGARAQDMPAQAQALVKKMKSTPTINIQEDWKLITLLIGNNDLCLYCEDPENYSTREYVKYIQHALDIFYEELPRVFINVVEVMELSGLLHDQGGKCAMPLAVQKNCSCLKRSQNLMAMQELKKVNGNLQSALSELSYWHRYMQREDFAVTVQPFFRNTFVPLDERGGLDLTFFSEDCFHFSVRGHAEMAIALWNNMLEPVGKKTTSNNFTYNRTKLKCPSPENPFLYTVRNSQILLDKAKENSNTLYWAVPVAAVGGLVVGILGMMLWRTVRLVQ

Target ORF information:

RefSeq Version	NM_138898.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phospholipase B1 (Plb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138898.1

ORF Insert Sequence:

ATGGAGTCAT GGCCAGGCGT TTCCCTGGTG GGACTGCTGC TGCTGCTACT GCTGGGACAA 
GGGCCCTCCC AAATCCACGG CTCTTCTGGA GAAAACACAT CGCAGCCCCA GCAAGTGTTT 
CGGACCCTGA AGAATTTTTC ATTCCCTTGC AAGCCAAAGA AGTTAGAACT GAGTGTGCTT 
TCTAAGTCAG TTCACTCTCT GAGACCCTCA GACATTAAAC TCGTGGCAGC CATCGGCAAC 
CTAGAAACTC CTCCAGCCCC TGGCTCAGGC GTGGTCAACA TGGAGAAACC TCAAAGCCTC 
GAGAGCGAAC TACAGAATGT GTGCATAGGA ATCATGACAG CCCTTTCAGA TATCATCAGA 
CATTTCAACC CTTCTGTTCT GATGCCCACG TGTTCTCCTG GGAAGGGTAC TGCAGGCCAC 
ACTACTATTG CCGAAGACTT GTGGATTCAG GCTAAAGAGC TGGTGAGGCA TCTGAAAGAC 
AACCCGGAAC TTGATTTTGA GAAGGACTGG AAACTCATCA CTGTGCTCTT CAGTAACACA 
AGCCAGTGCC ACCTGTGTTC CTCTGATCAG CAGAAAAGGC ACTTGATGAA GCACATGGAG 
ATGCTGTCAG GGGTGCTGGA TTACCTGCAT CGTGAGGTCC CCAGAGCGTT TGTGAATTTG 
GTGGATCTCT CTGAGGTTTT GACCATGGCT CAGCAGCATC AAGAGACTGG TTTCAGCCCT 
GCACCAGAGA TTTGCAAATG CTCAGAGGAA ATAACGAAGT TATCCAAGGC TGTCATGCAG 
TGGTCCTATC AGGAAGCCTG GGAAGATCTC CTGGCCTCCA GCAAGTTCAA TAAGCATGAG 
ACCTTCGCTG TGGTTTTCCA GTCTTTCTTC TCTGAGGTAG AACTACCCTT GGAGAGGCCC 
TCGCCCCAGG ATTCCACCAC GCTCGCCCTC AGGATCTGGA ATAGCATGAT GGAACCAGTG 
GGTCGAAAGG ATGGGACACT CAATGAAGCA GAGAGAAAAA CAATGAAATG TCCCTCTCAG 
GAGAGCCCCT ATCTGTTCAC CTACAGAAAT AGCAACTACC AGGCCAGACA GCTGAAACCC 
ATAGGAAAGT TTCAGATGAA AGAAGGAACA AAATTCACCT GTCCTGACAA AGACCCCTCG 
GACTCCATCC CCACAACAGT TCACAGGCTG AGGCCGGCTG ACATCAAGGT CATCGGAGCC 
ATGGGTGACT CGCTCACGGC AGGCAACGGG GCAGGGTCCA GCCCTGGGAA TGTCTTGGAT 
GTCTTAACTC AATACCGAGG CCTGTCGTGG AGTGTGGGCG GAGATGAGAC CATCGAGACC 
GTGACCACCC TAGCCAACAT CCTCCGGGAA TTCAACCCCT CCTTGAAGGG CTTCTCTGTT 
GGCACTGGGA AAGAAAACAC TCCCCGAGCA TCCTTCAACC AGGCCGTAGC AGGAGCCAAA 
TCTGATGGCT TAGCTGCCCA GGCCAAAAAG CTGGTGAGCC TGATGAAGGA TGACAAGACA 
ATAAACTTTC AGGAAGACTG GAAGATAATC ACTGTGTTTA TAGGAGGCAA CGACCTCTGT 
GGCTCCTGCA ATAACCTGGC TCGCTTTTCT CCCCAAACCT TCACAGACAA CATCAAGACG 
GCCCTGGACA TCCTCCATGC AGAGGTTCCC CGGGCCTTTG TGAACATGGT CTCGGTGATT 
GAGATCACCC CCTTGAGAGA ACTGTTCAAT GAACCTAAAG TCAGCTGCCC ACGGATGATC 
CTCAGGAGCC TGTGTCCTTG TGTCTTGAAC CTTGGTGAGA ACTCAGCAGA ACTTGCCCAA 
CTTGTGGAAA GGAACAGGCA GTATCAGGAA GAAACTGGAA AACTGATTGA GAGTGGCCGA 
TACGACACCA GGGATGACTT CACCGTGGTC CTCCAGCCCA TGTTTGAAAA TGTCGTCATG 
CCACGGACCC TGGAGGGCTT GCCCGACAGC TCTTTCTTTG CCCCTGACTG TTTCCACTTC 
AATGTCAAGA CTCACGCTCG CTCAGCCATC GCCCTCTGGA AGAACATGCT AGAACCTGTG 
GGCCGCAAGA CAAGACATCA GAATTTTGAA ATCAAGGTCC CTATCATGTG TCCAAACCAG 
ACCTCACCGT TTCTGAGCAC CACCAAGAAC AGCAACCTGG GACATGGAAC TTCGATGTCT 
TGTGAGGAGA AAGCCCCCTC TGCCTCACCA CCAACCTCAG TGCATACCCT GAGACCTGCA 
GACATCCAAG TTGTGGCAGC TCTGGGAGAC TCTGTGACTG CTGGCAATGG AATCAGCTCC 
CAAGAAGGTG ATCTCGCTGA TGTTACCACA CAGTATCGAG GACTGTCCTA CAGTGCTGGT 
GGGGACAAGT TCCTGGAGAA TGTGACCACC TTGCCCAACA TCCTCCGGGA ATTTAATGGA 
AATCTCACAG GCTACTCAGT CGGAACCGGT GACGTCAACT CTGCAAGCGC GTTCCTTAAC 
CAGGCTGTCC CTGGGGCAAA GGCTGAGAAC CTTGCAAGTC AAGTCCAGAC TCTGATTCAG 
AAGATGAAGA ATGACACCAG AGTGAACTTT CACCAAGACT GGAAGGTCAT CACTGTGATG 
ATTGGGGCCA GCGACTTGTG TGACTTCTGC AAGGATTCGA ATCGTTACTC TGCAGCCAAT 
TTTTCTGACC ATCTCCGCAA TGCCTTGGAC ATCCTGCATA AGGAGGTACC CAGAGCCCTG 
GTCAACCTTG TGGACTTCAT GAACCCCAGT ATCATTCGGC AAGTGTTCCT GAAGAACCCA 
GACAAGTGCC CTGTGAATCA GACCAGTGTC CTGTGCAACT GTGTTCTGAC CCCAGGGGAG 
GATTCCCATG AGCTGGCAAG GTTGGAGGCC TTCACCAAAT CCTACCAGAG TAGCATGCTT 
CAACTGGTTG AGTCAGGCCG CTACGACACC CGGGAGGATT TCTCTGTGGT ACTGCAGCCC 
TTTCTCTTCA ACATCAGGCT CCCCATCCTA GAGAATGGGA ATCCAGATAC ATCCTTCTTT 
GCCCCAGACT GCATCCTCCT AAGCCAGAAG TTCCACACTC AGCTCGCGAG AGCCCTTTGG 
GCCAATATGC TTGAACCCCT GGGAAAGAAA ATGGATACTT TGGACCCGAA AGAACTCATA 
GCTTTGGCCT GCCCCACCAA GGACAAGCCC TTCCTGAGAA CCTTCCGGAA CAGTAACTAC 
ACGTACCCTA TCAAGCCAGC CATTGAGAAT TGGGGCAGTG ACTTCCTGTG CACAGAGCAG 
AGTCCTTCCA GCAAGGTACC CACCTCAGTT CATGAGCTCC GACCATCAGA CATCAAGGTG 
GTGGCAGCAA TGGGTGACTT TCTGACGACA GCCACCGGAG CTCGACCAAG TGAGTCCAGC 
AGTCTAGACA CCCCCTGGAG GGGGCTGTCT TGGAGCATTG GAGGAGATGG AACGTTGGAG 
ACCCATACCA CACTGCCCAA CATCCTGAAG AAGTTCAACC CTTCCATCCT TGGATTCTCC 
ACCGGTACCC TGGAGAACAC GGCAGGATTA AATGTGGCAG AAGAAGGCGC CAGAGCTCAG 
GACATGCCGG CCCAGGCTCA GGCCCTGGTG AAGAAGATGA AAAGCACCCC TACAATCAAC 
ATACAGGAAG ACTGGAAGCT GATTACACTC CTCATCGGGA ACAACGACCT GTGTCTTTAC 
TGTGAGGATC CGGAGAACTA CTCAACCAGG GAGTATGTCA AGTACATCCA GCATGCCTTG 
GACATCTTCT ATGAGGAGCT TCCCAGGGTT TTCATCAACG TGGTGGAAGT CATGGAGCTG 
TCCGGTCTGC TCCACGACCA GGGCGGGAAA TGTGCCATGC CGTTGGCTGT CCAGAAAAAC 
TGCAGTTGCC TTAAACGCTC TCAAAACCTC ATGGCAATGC AGGAGCTGAA GAAAGTCAAC 
GGGAACCTCC AGAGTGCCCT CTCGGAGCTC TCCTACTGGC ATCGGTACAT GCAGCGTGAG 
GACTTTGCAG TCACTGTCCA GCCTTTCTTC CGGAATACCT TTGTCCCACT GGATGAGCGT 
GGGGGCCTCG ACCTCACTTT CTTCTCTGAA GACTGTTTCC ACTTCTCAGT CCGTGGGCAT 
GCTGAGATGG CCATTGCCCT CTGGAACAAC ATGCTGGAAC CAGTGGGCAA GAAGACAACC 
TCCAATAACT TCACATACAA CCGGACCAAA CTCAAGTGTC CCTCGCCTGA AAACCCTTTC 
CTCTACACTG TCCGGAACAG TCAGATTCTT CTAGACAAGG CTAAAGAAAA CTCCAATACA 
CTCTACTGGG CAGTGCCAGT GGCTGCAGTA GGTGGCCTGG TAGTTGGCAT CCTTGGAATG 
ATGTTGTGGA GAACTGTGAG ACTCGTCCAA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phospholipase B is activated in response to sterol removal and stimulates acrosome exocytosis in murine sperm.
The Journal of biological chemistry288(39)28104-15(2013 Sep)
Asano A,Nelson-Harrington JL,Travis AJ

Mechanisms involved in the intestinal absorption of dietary vitamin A and provitamin A carotenoids.
Biochimica et biophysica acta1821(1)70-7(2012 Jan)
Harrison EH

Identification of functional domains of rat intestinal phospholipase B/lipase. Its cDNA cloning, expression, and tissue distribution.
The Journal of biological chemistry273(4)2222-31(1998 Jan)
Takemori H,Zolotaryov FN,Ting L,Urbain T,Komatsubara T,Hatano O,Okamoto M,Tojo H

Purification and partial characterization of a retinyl ester hydrolase from the brush border of rat small intestine mucosa: probable identity with brush border phospholipase B.
Biochemistry33(9)2661-6(1994 Mar)
Rigtrup KM,Kakkad B,Ong DE

Further characterization of a novel phospholipase B (phospholipase A2--lysophospholipase) from intestinal brush-border membranes.
Biochemistry and cell biology = Biochimie et biologie cellulaire69(5-6)346-57(1991 May-Jun)
Pind S,Kuksis A

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Plb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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