Pcsk4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcsk4
Entrez Gene ID	171085
Full Name	proprotein convertase subtilisin/kexin type 4
General protein information	Preferred Names proprotein convertase subtilisin/kexin type 4 Names proprotein convertase subtilisin/kexin type 4 KEX2-like endoprotease 3 NEC 3 PC4 neuroendocrine convertase 3 prohormone convertase 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11
Summary	member of the Kex2/subtilisin-like proprotein convertases; may mediate the developmental maturation of germ cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133559.1	NP_598243.1	proprotein convertase subtilisin/kexin type 4 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Pcsk4	NP_032819.1
Rattus norvegicus (Norway rat)	Pcsk4	NP_598243.1
Pan troglodytes (chimpanzee)	PCSK4	XP_001172660.2
Saccharomyces cerevisiae (baker's yeast)	KEX2	NP_014161.1
Neurospora crassa	NCU03219	XP_965319.1
Xenopus tropicalis (tropical clawed frog)	pcsk4	NP_001072361.1
	PCSK4	XP_001095657.1
Bos taurus (cattle)	PCSK4	XP_005209386.1
Eremothecium gossypii	AGOS_ABL203W	NP_982744.2
Schizosaccharomyces pombe (fission yeast)	krp1	NP_594835.1
Magnaporthe oryzae (rice blast fungus)	MGG_15291	XP_003716137.1
Homo sapiens (human)	PCSK4	NP_060043.2
Canis lupus familiaris (dog)	PCSK4	XP_542201.3
Gallus gallus (chicken)	PCSK4	XP_003642925.2
Kluyveromyces lactis	KLLA0D19811g	XP_453942.1

Gene Ontology
Bibliography

Related articles in PubMed

Enzymatic activity of sperm proprotein convertase is important for mammalian fertilization.
Iamsaard S, Vanichviriyakit R, Hommalai G, Saewu A, Srakaew N, Withyachumnarnkul B, Basak A, Tanphaichitr N
Journal of cellular physiology226(11)2817-26(2011 Nov)

The precursor to the germ cell-specific PCSK4 proteinase is inefficiently activated in transfected somatic cells: evidence of interaction with the BiP chaperone.
Gyamera-Acheampong C, Sirois F, Denis NJ, Mishra P, Figeys D, Basak A, Mbikay M
Molecular and cellular biochemistry348(1-2)43-52(2011 Feb)

PCSK4-null sperm display enhanced protein tyrosine phosphorylation and ADAM2 proteolytic processing during in vitro capacitation.
Gyamera-Acheampong C, Vasilescu J, Figeys D, Mbikay M
Fertility and sterility93(4)1112-23(2010 Mar)

Sperm from mice genetically deficient for the PCSK4 proteinase exhibit accelerated capacitation, precocious acrosome reaction, reduced binding to egg zona pellucida, and impaired fertilizing ability.
Gyamera-Acheampong C, Tantibhedhyangkul J, Weerachatyanukul W, Tadros H, Xu H, van de Loo JW, Pelletier RM, Tanphaichitr N, Mbikay M
Biology of reproduction74(4)666-73(2006 Apr)

Role of pro-IGF-II processing by proprotein convertase 4 in human placental development.
Qiu Q, Basak A, Mbikay M, Tsang BK, Gruslin A
Proceedings of the National Academy of Sciences of the United States of America102(31)11047-52(2005 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Maturation by propeptide removal occurs very inefficiently when rat or human proPCSK4 is overexpressed in HEK293 cells.
Title: The precursor to the germ cell-specific PCSK4 proteinase is inefficiently activated in transfected somatic cells: evidence of interaction with the BiP chaperone.

The following Pcsk4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcsk4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00934	NM_133559.1 Latest version!	Rattus norvegicus proprotein convertase subtilisin/kexin type 4 (Pcsk4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcsk4 ( NM_133559.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_598243.1 Rattus norvegicus proprotein convertase subtilisin/kexin type 4 (Pcsk4), mRNA.

Price & Availability↑

CloneID	ORa00934
Clone ID Related Accession (Same CDS sequence)	NM_133559.1
Accession Version	NM_133559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Rattus norvegicus(Norway rat)
Product	proprotein convertase subtilisin/kexin type 4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L14937.1. ##Evidence-Data-START## Transcript exon combination :: L14937.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN02642708 [ECO:0000348] ##Evidence-Data-END##

ATGCGGCCCT CCCAGACAGC GCTGTGGCTG GGTCTGGTTT TGTCTTTGGC CCTCCTGGCT 
GTGGGGTGGG CCTCAGCCCG ACCACCCATC TATGTCAGCA GCTGGGCAGT GCGGGTGACC 
AAAGGTTACC AGGAGGCTGA GCGCCTGGCA CGTAAATTTG GCTTCGTCAA CCTGGGACAG 
ATCTTTCCTG ATGACCAGTA TTTCCATCTG AGGCACCGGG GTGTGGCCCA GCAGTCCCTG 
ACTCCGCACT GGGGCCACCG TCTGCGCCTG AAGAAAGAGC CCAAGGTGCG GTGGTTTGAG 
CAGCAGACTT TGAGGCGGCG GGTGAAGCGC TCCCTGGTGG TACCCACAGA CCCCTGGTTT 
TCCAAGCAGT GGTACATGAA CAAGGAGATA GAACAAGATC TCAACATCCT AAAGGTTTGG 
AACCAGGGAC TGACTGGCCG GGGAGTGGTG GTCTCCATCT TGGATGATGG CATTGAGAAG 
GACCATCCGG ACCTCTGGGC TAATTATGAC CCCCTGGCCA GCTATGACTT CAATGATTAC 
GACCCAGATC CCCAGCCTCG ATACACACCC AACGATGAGA ACCGGCATGG AACACGCTGC 
GCTGGGGAGG TGTCTGCCAC AGCAAACAAC GGTTTCTGTG GTGCCGGTGT GGCCTTCAAT 
GCCAGAATTG GAGGCGTGCG CATGTTGGAT GGAGCCATCA CTGACATCGT GGAGGCTCAG 
TCCCTCAGCC TGCAGCCGCA ACACATACAC ATCTATAGCG CCAGTTGGGG CCCCGAGGAT 
GATGGGCGCA CAGTGGACGG ACCCGGCCTC CTCACGCAGG AGGCCTTCAG GCGTGGTGTA 
ACCAAGGGCC GCCAAGGGCT GGGCACGCTG TTCATCTGGG CCTCGGGAAA CGGTGGCCTC 
CACTACGACA ACTGCAATTG TGACGGCTAC ACCAACAGCA TCCACACGCT GTCAGTGGGC 
AGTACCACGC GGCAGGGCCG AGTGCCCTGG TACAGCGAGG CCTGCGCCTC CACGTTCACC 
ACCACCTTCA GCAGCGGTGT GGTCACCGAC CCACAGATCG TCACCACGGA CCTACACCAT 
CAATGCACCG ACAAGCACAC GGGCACCTCG GCCTCCGCCC CGCTGGCCGC TGGCATGATC 
GCCCTGGCGC TGGAGGCCAA CCCGCTGCTG ACCTGGAGGG ACCTGCAGCA CCTGGTGGTC 
CGCGCGTCCA GGCCGGCGCA GCTGCAGGCG GAGGACTGGA GGATCAACGG CGTGGGGCGC 
CAAGTGAGCC ACCACTATGG CTATGGGCTG CTGGACGCGG GGCTGCTGGT AGACCTGGCT 
CGCGTGTGGC TCCCTACTAA GCCTCAGAAG AAATGCACCA TTCGGGTGGT GCACACCCCA 
ACCCCCATCC TGCCTCGGAT GCTGGTGCCA AAGAACGTGA CTGTATGCTG CGATGGCTCG 
CGCCGCCGCC TCATCCGCTC GCTAGAGCAT GTTCAGGTCC AGCTGTCGCT CTCCTACAGC 
CGCCGCGGGG ACCTGGAGAT CTTCCTCACC AGCCCCATGG GCACGCGCTC CACGCTTGTG 
GCCATCAGAC CCTTGGATAT CAGCGGCCAA GGCTACAACA ACTGGATCTT CATGTCTACT 
CACTACTGGG ATGAGGACCC GCAGGGCCTG TGGACCCTGG GGCTGGAGAA TAAGGGCTAC 
TATTATAACA CAGGAACTCT GTACTACTGC ACGCTGCTGC TGTATGGGAC GGCAGAGGAC 
ATGACAGCGC GGCCCCAGAC CCCCCAGGTG ACCAGCTGCG CGCACGCATG TGCAGAGGGA 
CACAGAGGGG CTGTGCCAGG AAAGTCATTG TCCCCTCTCC ATTGTGGCAG AACTCTGCCT 
CATCTCCAGC AAGCAGTGGT GGTGGCTCTA CAGCCACACA CAGCAGCCAG TGACCAAGGG 
ACAGGACAGC TGTCACCCTC CTACCACACC TGCTCGGCAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598243.1
CDS	1..1965
Translation	MRPSQTALWLGLVLSLALLAVGWASARPPIYVSSWAVRVTKGYQEAERLARKFGFVNLGQIFPDDQYFHLRHRGVAQQSLTPHWGHRLRLKKEPKVRWFEQQTLRRRVKRSLVVPTDPWFSKQWYMNKEIEQDLNILKVWNQGLTGRGVVVSILDDGIEKDHPDLWANYDPLASYDFNDYDPDPQPRYTPNDENRHGTRCAGEVSATANNGFCGAGVAFNARIGGVRMLDGAITDIVEAQSLSLQPQHIHIYSASWGPEDDGRTVDGPGLLTQEAFRRGVTKGRQGLGTLFIWASGNGGLHYDNCNCDGYTNSIHTLSVGSTTRQGRVPWYSEACASTFTTTFSSGVVTDPQIVTTDLHHQCTDKHTGTSASAPLAAGMIALALEANPLLTWRDLQHLVVRASRPAQLQAEDWRINGVGRQVSHHYGYGLLDAGLLVDLARVWLPTKPQKKCTIRVVHTPTPILPRMLVPKNVTVCCDGSRRRLIRSLEHVQVQLSLSYSRRGDLEIFLTSPMGTRSTLVAIRPLDISGQGYNNWIFMSTHYWDEDPQGLWTLGLENKGYYYNTGTLYYCTLLLYGTAEDMTARPQTPQVTSCAHACAEGHRGAVPGKSLSPLHCGRTLPHLQQAVVVALQPHTAASDQGTGQLSPSYHTCSAA

Target ORF information:

RefSeq Version	NM_133559.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus proprotein convertase subtilisin/kexin type 4 (Pcsk4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133559.1

ORF Insert Sequence:

ATGCGGCCCT CCCAGACAGC GCTGTGGCTG GGTCTGGTTT TGTCTTTGGC CCTCCTGGCT 
GTGGGGTGGG CCTCAGCCCG ACCACCCATC TATGTCAGCA GCTGGGCAGT GCGGGTGACC 
AAAGGTTACC AGGAGGCTGA GCGCCTGGCA CGTAAATTTG GCTTCGTCAA CCTGGGACAG 
ATCTTTCCTG ATGACCAGTA TTTCCATCTG AGGCACCGGG GTGTGGCCCA GCAGTCCCTG 
ACTCCGCACT GGGGCCACCG TCTGCGCCTG AAGAAAGAGC CCAAGGTGCG GTGGTTTGAG 
CAGCAGACTT TGAGGCGGCG GGTGAAGCGC TCCCTGGTGG TACCCACAGA CCCCTGGTTT 
TCCAAGCAGT GGTACATGAA CAAGGAGATA GAACAAGATC TCAACATCCT AAAGGTTTGG 
AACCAGGGAC TGACTGGCCG GGGAGTGGTG GTCTCCATCT TGGATGATGG CATTGAGAAG 
GACCATCCGG ACCTCTGGGC TAATTATGAC CCCCTGGCCA GCTATGACTT CAATGATTAC 
GACCCAGATC CCCAGCCTCG ATACACACCC AACGATGAGA ACCGGCATGG AACACGCTGC 
GCTGGGGAGG TGTCTGCCAC AGCAAACAAC GGTTTCTGTG GTGCCGGTGT GGCCTTCAAT 
GCCAGAATTG GAGGCGTGCG CATGTTGGAT GGAGCCATCA CTGACATCGT GGAGGCTCAG 
TCCCTCAGCC TGCAGCCGCA ACACATACAC ATCTATAGCG CCAGTTGGGG CCCCGAGGAT 
GATGGGCGCA CAGTGGACGG ACCCGGCCTC CTCACGCAGG AGGCCTTCAG GCGTGGTGTA 
ACCAAGGGCC GCCAAGGGCT GGGCACGCTG TTCATCTGGG CCTCGGGAAA CGGTGGCCTC 
CACTACGACA ACTGCAATTG TGACGGCTAC ACCAACAGCA TCCACACGCT GTCAGTGGGC 
AGTACCACGC GGCAGGGCCG AGTGCCCTGG TACAGCGAGG CCTGCGCCTC CACGTTCACC 
ACCACCTTCA GCAGCGGTGT GGTCACCGAC CCACAGATCG TCACCACGGA CCTACACCAT 
CAATGCACCG ACAAGCACAC GGGCACCTCG GCCTCCGCCC CGCTGGCCGC TGGCATGATC 
GCCCTGGCGC TGGAGGCCAA CCCGCTGCTG ACCTGGAGGG ACCTGCAGCA CCTGGTGGTC 
CGCGCGTCCA GGCCGGCGCA GCTGCAGGCG GAGGACTGGA GGATCAACGG CGTGGGGCGC 
CAAGTGAGCC ACCACTATGG CTATGGGCTG CTGGACGCGG GGCTGCTGGT AGACCTGGCT 
CGCGTGTGGC TCCCTACTAA GCCTCAGAAG AAATGCACCA TTCGGGTGGT GCACACCCCA 
ACCCCCATCC TGCCTCGGAT GCTGGTGCCA AAGAACGTGA CTGTATGCTG CGATGGCTCG 
CGCCGCCGCC TCATCCGCTC GCTAGAGCAT GTTCAGGTCC AGCTGTCGCT CTCCTACAGC 
CGCCGCGGGG ACCTGGAGAT CTTCCTCACC AGCCCCATGG GCACGCGCTC CACGCTTGTG 
GCCATCAGAC CCTTGGATAT CAGCGGCCAA GGCTACAACA ACTGGATCTT CATGTCTACT 
CACTACTGGG ATGAGGACCC GCAGGGCCTG TGGACCCTGG GGCTGGAGAA TAAGGGCTAC 
TATTATAACA CAGGAACTCT GTACTACTGC ACGCTGCTGC TGTATGGGAC GGCAGAGGAC 
ATGACAGCGC GGCCCCAGAC CCCCCAGGTG ACCAGCTGCG CGCACGCATG TGCAGAGGGA 
CACAGAGGGG CTGTGCCAGG AAAGTCATTG TCCCCTCTCC ATTGTGGCAG AACTCTGCCT 
CATCTCCAGC AAGCAGTGGT GGTGGCTCTA CAGCCACACA CAGCAGCCAG TGACCAAGGG 
ACAGGACAGC TGTCACCCTC CTACCACACC TGCTCGGCAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enzymatic activity of sperm proprotein convertase is important for mammalian fertilization.
Journal of cellular physiology226(11)2817-26(2011 Nov)
Iamsaard S,Vanichviriyakit R,Hommalai G,Saewu A,Srakaew N,Withyachumnarnkul B,Basak A,Tanphaichitr N

The precursor to the germ cell-specific PCSK4 proteinase is inefficiently activated in transfected somatic cells: evidence of interaction with the BiP chaperone.
Molecular and cellular biochemistry348(1-2)43-52(2011 Feb)
Gyamera-Acheampong C,Sirois F,Denis NJ,Mishra P,Figeys D,Basak A,Mbikay M

PCSK4-null sperm display enhanced protein tyrosine phosphorylation and ADAM2 proteolytic processing during in vitro capacitation.
Fertility and sterility93(4)1112-23(2010 Mar)
Gyamera-Acheampong C,Vasilescu J,Figeys D,Mbikay M

Sperm from mice genetically deficient for the PCSK4 proteinase exhibit accelerated capacitation, precocious acrosome reaction, reduced binding to egg zona pellucida, and impaired fertilizing ability.
Biology of reproduction74(4)666-73(2006 Apr)
Gyamera-Acheampong C,Tantibhedhyangkul J,Weerachatyanukul W,Tadros H,Xu H,van de Loo JW,Pelletier RM,Tanphaichitr N,Mbikay M

Role of pro-IGF-II processing by proprotein convertase 4 in human placental development.
Proceedings of the National Academy of Sciences of the United States of America102(31)11047-52(2005 Aug)
Qiu Q,Basak A,Mbikay M,Tsang BK,Gruslin A

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Pcsk4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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