St14 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	St14
Entrez Gene ID	114093
Full Name	suppression of tumorigenicity 14
Synonyms	MT-SP1
General protein information	Preferred Names suppression of tumorigenicity 14 Names suppressor of tumorigenicity 14 protein matriptase membrane-type serine protease suppression of tumorigenicity 14 (colon carcinoma) suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q13
Summary	regulates the cell-substratum adhesion by controlling the intestinal epithelial turnover [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053635.2	NP_446087.1	suppressor of tumorigenicity 14 protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	ST14	NP_068813.1
Xenopus tropicalis (tropical clawed frog)	st14	NP_001005075.1
	LOC718848	XP_001112126.2
Pan troglodytes (chimpanzee)	ST14	NP_001189434.1
Canis lupus familiaris (dog)	ST14	XP_546396.4
Bos taurus (cattle)	ST14	NP_001070006.1
Gallus gallus (chicken)	ST14	XP_417872.4
Mus musculus (house mouse)	St14	NP_035306.2
Rattus norvegicus (Norway rat)	St14	NP_446087.1
Danio rerio (zebrafish)	st14a	NP_001035441.2

Gene Ontology
Bibliography

Related articles in PubMed

Roles of CUB and LDL receptor class A domain repeats of a transmembrane serine protease matriptase in its zymogen activation.
Inouye K, Tomoishi M, Yasumoto M, Miyake Y, Kojima K, Tsuzuki S, Fushiki T
Journal of biochemistry153(1)51-61(2013 Jan)

Identification of the matriptase second CUB domain as the secondary site for interaction with hepatocyte growth factor activator inhibitor type-1.
Inouye K, Tsuzuki S, Yasumoto M, Kojima K, Mochida S, Fushiki T
The Journal of biological chemistry285(43)33394-403(2010 Oct)

Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome.
Sales KU, Masedunskas A, Bey AL, Rasmussen AL, Weigert R, List K, Szabo R, Overbeek PA, Bugge TH
Nature genetics42(8)676-83(2010 Aug)

Hepsin activates prostasin and cleaves the extracellular domain of the epidermal growth factor receptor.
Chen M, Chen LM, Lin CY, Chai KX
Molecular and cellular biochemistry337(1-2)259-66(2010 Apr)

The occurrence of matriptase C-terminal fragments on the apical and basolateral sides of Madin-Darby canine kidney epithelial cells.
Tsuzuki S, Miyake Y, Inouye K, Fushiki T
Bioscience, biotechnology, and biochemistry73(11)2538-40(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

investigation of protein structure of matriptase and the structural effects on kinetics of proteolysis and zymogen activation
Title: Roles of CUB and LDL receptor class A domain repeats of a transmembrane serine protease matriptase in its zymogen activation.

Identification of the matriptase second CUB domain as the secondary site for interaction with hepatocyte growth factor activator inhibitor type-1.
Title: Identification of the matriptase second CUB domain as the secondary site for interaction with hepatocyte growth factor activator inhibitor type-1.

It is concluded that N-glycosylation site at Asn772 of matriptase is required for the zymogen activation because it plays an important role in rendering this protease catalytically competent in the cellular environment.
Title: The role of asparagine-linked glycosylation site on the catalytic domain of matriptase in its zymogen activation.

Matriptase C-terminal fragments containing the catalytic serine protease domain were found to occur on the apical and basolateral sides of Madin-Darby canine kidney epithelial cells transfected with a cDNA encoding the protease.
Title: The occurrence of matriptase C-terminal fragments on the apical and basolateral sides of Madin-Darby canine kidney epithelial cells.

the activity of this protease was found to depend solely on the catalytic domain
Title: The activity of a type II transmembrane serine protease, matriptase, is dependent solely on the catalytic domain.

The following St14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the St14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10151	NM_053635.2 Latest version!	Rattus norvegicus suppression of tumorigenicity 14 (St14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

St14 ( NM_053635.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446087.1 Rattus norvegicus suppression of tumorigenicity 14 (St14), mRNA.

Price & Availability↑

CloneID	ORa10151
Clone ID Related Accession (Same CDS sequence)	NM_053635.2
Accession Version	NM_053635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	suppressor of tumorigenicity 14 protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB049189.1. On or before Dec 17, 2003 this sequence version replaced XM_346821.1, NM_053635.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB049189.1, AB037898.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAACA ATCGGGGCCG CAAGGCCGGA GGGGGCTCGC AGGACTTCGG CGCCGGACTC 
AAGTACAACT CCCGGCTAGA GAACATGAAT GGCTTTGAGG AGGGTGTGGA GTTCCTGCCT 
GTGAACAACG CCAAGCAGGT GGAGAAGCGA GGCCCCAGGC GCTGGGTGGT GATGGTGGCC 
GTAGTGTTCA GCTTCCTTTT GCTCTCGCTC ATGGCCGGCT TGCTGGTGTG GCACTTCCAT 
TACCGGAATG TGAGGATCCA AAAGGTCTTC AATGGCCATC TGAGGATCAC GAATGAGAAC 
TTTCTGGACG CGTATGAGAA CTCCACCTCC ACGGAGTTCA TCAGCCTGGC CAGTCAGGTG 
AAGGAAGCGC TGAAGCTGAT GTACAGTGAA GTCCCTGTCC TGGGCCCCTA CCACAAGAAA 
TCGACTGTAA CTGCCTTCAG TGAGGGCAGC GTCATTGCCT ACTACTGGTC AGAGTTCAGC 
ATCCCCCCAC ACCTGGAGGA AGAGGTTGAT CGCGCCATGG CTGTGGAGCG AGTTGTCACA 
CTGCCACCCC GAGCACGGGC ACTGAAATCC TTCGTGCTGA CCTCTGTGGT GGCCTTCCCC 
ATTGACCCCA GAATGCTCCA GAGGACTCAG GACAACAGCT GCAGTTTTGC CCTCCATGCC 
CGTGGCAGGA CAGTGACACG TTTCACTACC CCTGGCTTCC CTAACAGTCC CTACCCAGCT 
CATGCCCGCT GCCAGTGGGT CCTGCGGGGG GACGCGGACT CTGTGCTGAG CCTCACCTTC 
CGAAGTTTTG ATGTCGCGCC CTGTGATGGG CATGACAGTG ACCTGGTCAC AGTGTATGAC 
AGCCTGAGCC CCATGGAGCC CCATGCTGTG GTGCGGCTGT GTGGCACCTT CTCGCCCTCC 
TACAACCTGA CTTTCCTCTC CTCCCAGAAC GTCTTCCTTG TTACGCTGAT AACCAACACT 
GACCGGCGAC ATCCTGGCTT TGAGGCCACT TTCTTCCAGC TGCCCAAGAT GAGCAGCTGT 
GGCGGCCTTT TGAGTGAAGC CCAAGGGACG TTTAGCAGCC CCTACTATCC AGGCCATTAC 
CCGCCCAACA TCAACTGCAC ATGGAATATC AAGGTGCCCA ACAACCGGAA CGTGAAAGTA 
CGCTTCAAAC TCTTCTATCT GGTGGACCCC AACATACCAG TGGGAAGCTG CACCAAGGAC 
TATGTGGAGA TCAACGGGGA GAAGTTCTGC GGTGAGAGGT CCCAGTTTGT GGTGAGCAGC 
AACAGCAGCA AGATTACGGT CCACTTCCAT TCTGATCACT CGTACACGGA CACCGGGTTC 
CTGGCTGAGT ATCTCTCCTA CGACTCCAAT GACCCATGCC CAGGGATGTT CATGTGTAAG 
ACTGGACGGT GCATCCGCAA GGACTTGCGC TGCGATGGCT GGGCTGACTG CCCGGACTAC 
AGTGATGAGC GTCACTGCCG ATGCAACGCT ACCCACCAGT TCATGTGCAA GAACCAGTTC 
TGCAAGCCCC TCTTCTGGGT CTGTGACAGT GTCAACGACT GTGGGGATGG CAGCGATGAG 
GAGGGCTGCA GTTGTCCTGC CGGGAGTTTC AAGTGTTCCA ATGGGAAGTG TCTCCCTCAG 
AGCCAGCAGT GTAATGGGAA GGACGACTGT GGAGATGGGT CTGACGAGGC CTCATGTGAC 
AATGTGAATG CCGTCTCTTG CACCAAATAT ACCTACCGCT GCCAAAATGG CCTCTGTCTG 
AACAAGGGCA ACCCTGAGTG TGATGGGAAG AAGGACTGCA GCGATGGCTC GGATGAGAAA 
AACTGTGACT GTGGGCTGCG ATCCTTTACC AAACAGGCTC GAGTGGTTGG TGGCACGAAT 
GCGGACGAGG GCGAGTGGCC CTGGCAGGTG AGCCTCCACG CTCTGGGCCA GGGCCACTTG 
TGTGGGGCCT CGCTCATCTC TCCTGACTGG CTGGTCTCTG CAGCTCATTG CTTTCAGGAT 
GAGACAATTT TCAAGTACTC AGACCACACC ATGTGGACAG CCTTCCTCGG TCTGCTGGAC 
CAGAGCAAGC GCAGTGCCTC TGGGGTACAG GAGCACAAGC TCAAACGTAT CATCACTCAC 
CCTTCCTTTA ATGACTTTAC CTTCGACTAT GACATTGCCT TGCTGGAGCT GGAGAAGCCG 
GCAGAGTACA GCACTGTCGT GCGCCCCATC TGCCTGCCTG ATAATACCCA TGTCTTTCCT 
GCCGGCAAAG CCATCTGGGT CACAGGCTGG GGCCACACGA AAGAAGGAGG AACTGGAGCA 
CTGATCCTGC AGAAGGGAGA GATCCGGGTC ATCAACCAGA CCACCTGTGA GGAACTCTTG 
CCGCAGCAGA TCACCCCACG AATGATGTGT GTGGGTTTCC TCAGTGGGGG TGTGGACTCC 
TGCCAGGGTG ATTCCGGTGG CCCCTTGTCC AGTGTGGAGA AAGATGGGCG AATCTTCCAG 
GCTGGTGTGG TGAGCTGGGG TGAAGGCTGC GCTCAGAGGA ACAAGCCAGG CGTGTACACA 
AGGATCCCTG AAGTTCGGGA CTGGATCAAA GAGCAAACTG GGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446087.1
CDS	127..2694
Translation	MGNNRGRKAGGGSQDFGAGLKYNSRLENMNGFEEGVEFLPVNNAKQVEKRGPRRWVVMVAVVFSFLLLSLMAGLLVWHFHYRNVRIQKVFNGHLRITNENFLDAYENSTSTEFISLASQVKEALKLMYSEVPVLGPYHKKSTVTAFSEGSVIAYYWSEFSIPPHLEEEVDRAMAVERVVTLPPRARALKSFVLTSVVAFPIDPRMLQRTQDNSCSFALHARGRTVTRFTTPGFPNSPYPAHARCQWVLRGDADSVLSLTFRSFDVAPCDGHDSDLVTVYDSLSPMEPHAVVRLCGTFSPSYNLTFLSSQNVFLVTLITNTDRRHPGFEATFFQLPKMSSCGGLLSEAQGTFSSPYYPGHYPPNINCTWNIKVPNNRNVKVRFKLFYLVDPNIPVGSCTKDYVEINGEKFCGERSQFVVSSNSSKITVHFHSDHSYTDTGFLAEYLSYDSNDPCPGMFMCKTGRCIRKDLRCDGWADCPDYSDERHCRCNATHQFMCKNQFCKPLFWVCDSVNDCGDGSDEEGCSCPAGSFKCSNGKCLPQSQQCNGKDDCGDGSDEASCDNVNAVSCTKYTYRCQNGLCLNKGNPECDGKKDCSDGSDEKNCDCGLRSFTKQARVVGGTNADEGEWPWQVSLHALGQGHLCGASLISPDWLVSAAHCFQDETIFKYSDHTMWTAFLGLLDQSKRSASGVQEHKLKRIITHPSFNDFTFDYDIALLELEKPAEYSTVVRPICLPDNTHVFPAGKAIWVTGWGHTKEGGTGALILQKGEIRVINQTTCEELLPQQITPRMMCVGFLSGGVDSCQGDSGGPLSSVEKDGRIFQAGVVSWGEGCAQRNKPGVYTRIPEVRDWIKEQTGV

Target ORF information:

RefSeq Version	NM_053635.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus suppression of tumorigenicity 14 (St14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053635.2

ORF Insert Sequence:

ATGGGGAACA ATCGGGGCCG CAAGGCCGGA GGGGGCTCGC AGGACTTCGG CGCCGGACTC 
AAGTACAACT CCCGGCTAGA GAACATGAAT GGCTTTGAGG AGGGTGTGGA GTTCCTGCCT 
GTGAACAACG CCAAGCAGGT GGAGAAGCGA GGCCCCAGGC GCTGGGTGGT GATGGTGGCC 
GTAGTGTTCA GCTTCCTTTT GCTCTCGCTC ATGGCCGGCT TGCTGGTGTG GCACTTCCAT 
TACCGGAATG TGAGGATCCA AAAGGTCTTC AATGGCCATC TGAGGATCAC GAATGAGAAC 
TTTCTGGACG CGTATGAGAA CTCCACCTCC ACGGAGTTCA TCAGCCTGGC CAGTCAGGTG 
AAGGAAGCGC TGAAGCTGAT GTACAGTGAA GTCCCTGTCC TGGGCCCCTA CCACAAGAAA 
TCGACTGTAA CTGCCTTCAG TGAGGGCAGC GTCATTGCCT ACTACTGGTC AGAGTTCAGC 
ATCCCCCCAC ACCTGGAGGA AGAGGTTGAT CGCGCCATGG CTGTGGAGCG AGTTGTCACA 
CTGCCACCCC GAGCACGGGC ACTGAAATCC TTCGTGCTGA CCTCTGTGGT GGCCTTCCCC 
ATTGACCCCA GAATGCTCCA GAGGACTCAG GACAACAGCT GCAGTTTTGC CCTCCATGCC 
CGTGGCAGGA CAGTGACACG TTTCACTACC CCTGGCTTCC CTAACAGTCC CTACCCAGCT 
CATGCCCGCT GCCAGTGGGT CCTGCGGGGG GACGCGGACT CTGTGCTGAG CCTCACCTTC 
CGAAGTTTTG ATGTCGCGCC CTGTGATGGG CATGACAGTG ACCTGGTCAC AGTGTATGAC 
AGCCTGAGCC CCATGGAGCC CCATGCTGTG GTGCGGCTGT GTGGCACCTT CTCGCCCTCC 
TACAACCTGA CTTTCCTCTC CTCCCAGAAC GTCTTCCTTG TTACGCTGAT AACCAACACT 
GACCGGCGAC ATCCTGGCTT TGAGGCCACT TTCTTCCAGC TGCCCAAGAT GAGCAGCTGT 
GGCGGCCTTT TGAGTGAAGC CCAAGGGACG TTTAGCAGCC CCTACTATCC AGGCCATTAC 
CCGCCCAACA TCAACTGCAC ATGGAATATC AAGGTGCCCA ACAACCGGAA CGTGAAAGTA 
CGCTTCAAAC TCTTCTATCT GGTGGACCCC AACATACCAG TGGGAAGCTG CACCAAGGAC 
TATGTGGAGA TCAACGGGGA GAAGTTCTGC GGTGAGAGGT CCCAGTTTGT GGTGAGCAGC 
AACAGCAGCA AGATTACGGT CCACTTCCAT TCTGATCACT CGTACACGGA CACCGGGTTC 
CTGGCTGAGT ATCTCTCCTA CGACTCCAAT GACCCATGCC CAGGGATGTT CATGTGTAAG 
ACTGGACGGT GCATCCGCAA GGACTTGCGC TGCGATGGCT GGGCTGACTG CCCGGACTAC 
AGTGATGAGC GTCACTGCCG ATGCAACGCT ACCCACCAGT TCATGTGCAA GAACCAGTTC 
TGCAAGCCCC TCTTCTGGGT CTGTGACAGT GTCAACGACT GTGGGGATGG CAGCGATGAG 
GAGGGCTGCA GTTGTCCTGC CGGGAGTTTC AAGTGTTCCA ATGGGAAGTG TCTCCCTCAG 
AGCCAGCAGT GTAATGGGAA GGACGACTGT GGAGATGGGT CTGACGAGGC CTCATGTGAC 
AATGTGAATG CCGTCTCTTG CACCAAATAT ACCTACCGCT GCCAAAATGG CCTCTGTCTG 
AACAAGGGCA ACCCTGAGTG TGATGGGAAG AAGGACTGCA GCGATGGCTC GGATGAGAAA 
AACTGTGACT GTGGGCTGCG ATCCTTTACC AAACAGGCTC GAGTGGTTGG TGGCACGAAT 
GCGGACGAGG GCGAGTGGCC CTGGCAGGTG AGCCTCCACG CTCTGGGCCA GGGCCACTTG 
TGTGGGGCCT CGCTCATCTC TCCTGACTGG CTGGTCTCTG CAGCTCATTG CTTTCAGGAT 
GAGACAATTT TCAAGTACTC AGACCACACC ATGTGGACAG CCTTCCTCGG TCTGCTGGAC 
CAGAGCAAGC GCAGTGCCTC TGGGGTACAG GAGCACAAGC TCAAACGTAT CATCACTCAC 
CCTTCCTTTA ATGACTTTAC CTTCGACTAT GACATTGCCT TGCTGGAGCT GGAGAAGCCG 
GCAGAGTACA GCACTGTCGT GCGCCCCATC TGCCTGCCTG ATAATACCCA TGTCTTTCCT 
GCCGGCAAAG CCATCTGGGT CACAGGCTGG GGCCACACGA AAGAAGGAGG AACTGGAGCA 
CTGATCCTGC AGAAGGGAGA GATCCGGGTC ATCAACCAGA CCACCTGTGA GGAACTCTTG 
CCGCAGCAGA TCACCCCACG AATGATGTGT GTGGGTTTCC TCAGTGGGGG TGTGGACTCC 
TGCCAGGGTG ATTCCGGTGG CCCCTTGTCC AGTGTGGAGA AAGATGGGCG AATCTTCCAG 
GCTGGTGTGG TGAGCTGGGG TGAAGGCTGC GCTCAGAGGA ACAAGCCAGG CGTGTACACA 
AGGATCCCTG AAGTTCGGGA CTGGATCAAA GAGCAAACTG GGGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Roles of CUB and LDL receptor class A domain repeats of a transmembrane serine protease matriptase in its zymogen activation.
Journal of biochemistry153(1)51-61(2013 Jan)
Inouye K,Tomoishi M,Yasumoto M,Miyake Y,Kojima K,Tsuzuki S,Fushiki T

Identification of the matriptase second CUB domain as the secondary site for interaction with hepatocyte growth factor activator inhibitor type-1.
The Journal of biological chemistry285(43)33394-403(2010 Oct)
Inouye K,Tsuzuki S,Yasumoto M,Kojima K,Mochida S,Fushiki T

Matriptase initiates activation of epidermal pro-kallikrein and disease onset in a mouse model of Netherton syndrome.
Nature genetics42(8)676-83(2010 Aug)
Sales KU,Masedunskas A,Bey AL,Rasmussen AL,Weigert R,List K,Szabo R,Overbeek PA,Bugge TH

Hepsin activates prostasin and cleaves the extracellular domain of the epidermal growth factor receptor.
Molecular and cellular biochemistry337(1-2)259-66(2010 Apr)
Chen M,Chen LM,Lin CY,Chai KX

The occurrence of matriptase C-terminal fragments on the apical and basolateral sides of Madin-Darby canine kidney epithelial cells.
Bioscience, biotechnology, and biochemistry73(11)2538-40(2009 Nov)
Tsuzuki S,Miyake Y,Inouye K,Fushiki T

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St14 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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