LOC110774500 cDNA ORF clone, Prunus avium(sweet cherry)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110774500
Entrez Gene ID	110774500
Full Name	DNA repair protein RAD50
Gene Type	protein-coding
Organism	Prunus avium(sweet cherry)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021979043.1	XP_021834735.1	DNA repair protein RAD50

Pathways from BioSystems
Gene Ontology

The following LOC110774500 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110774500 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPr123385	XM_021979043.1 Latest version!	Prunus avium DNA repair protein RAD50 (LOC110774500), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110774500 ( XM_021979043.1 ) cDNA ORF clone, Prunus avium(sweet cherry) -> XP_021834735.1 Prunus avium DNA repair protein RAD50 (LOC110774500), mRNA.

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CloneID	OPr123385
Clone ID Related Accession (Same CDS sequence)	XM_021979043.1
Accession Version	XM_021979043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-07-25
Organism	Prunus avium(sweet cherry)
Product	DNA repair protein RAD50
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018921329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Prunus avium Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTACGG TGGACAAGAT GCTGATAAAG GGAATTCGGA GCTTCGATCC GGAGAACAAG 
CACGTCATCA CCTTCTTCAA GCCCTTAACC CTAATCGTCG GCCCAAACGG CGCCGGAAAA 
ACCACCATTA TCGAGTGCTT GAAGCTCTCT TGCTCCGGCG AGTTGCCTCC CAATGCTAGG 
TCTGGCCACA GCTTCATCCA TGACCCGAAA GTTGCCGGCG AAACCGAGAC CAAAGCCCAG 
ATTAAGCTTC GATTCAGAAC CGCAGCGGGT AAAGATGTGG TGTGCATTAG GTCATTTCAG 
CTCACGCAGA AAGCCTCGAA AATGGAATTC AAAGCCATCG ACAGTGTCCT TCAAACCATT 
AATCCCCACA CGGGAGAGAA AGTTTGCCTT AGCTATAGAT GTGCTGATAT GGATAGGGAA 
ATCCCTGCTT TAATGGGTGT GTCAAAGGCT ATTTTGGAAA ATGTTATATT TGTGCACCAA 
GATGAAGCCA ATTGGCCTTT GCAAGATCCT TCCACATTGA AGAAAAAGTT TGATGATATC 
TTCTCTGCTA CCCGATATAC CAAGGCATTG GAGGTGATAA AAAAACTTCA CAAGGATCAA 
GCTCAAGAAA TAAAGACCTA CAAGCTAAAA TTGGAGAATC TTCAGACCTT GAAAGATGTT 
GCATATAAGC TTCGAGAGAG TATTTCTAAA GACCAAGAAA AAACAGAATC TGTGAAAAGT 
CAGATGCAAG AATTGGAGGG AAGCATCAGA GACGTGGATG CTAAAATTCG TTATACTGAA 
GCAACACTGA AAGATTTGCG GGAGCTTCAG GACCAGATAT CGATGAAGAC TGCTGTAAGA 
AGCACTCTCT TTAAGGAGCA GCAGAAAAAG TACGCAGATC TTGCTGAGGA GAACGAAGAC 
ACTGATGAAG AATTGAAGGA ATGGAAAACC AAGTTTGAAG AAAGGATTGC ACTTTTGGAA 
ACTAAAATTA GCAAGTTAGA GAGGGAAATG GAGGACACAG AGACCAAAAG TGGAGTCCTT 
AAGAAGACAC TAGAGAAATC TATATGGGAG ATTAGCAAGC TTCAAACCGA AGCTGAAGTT 
CATAGGTCCT CAAAGAGCGA GAGAGATTCT ATCATTCAAA ACTTTTTTAC AAGGCACAAT 
TTAGGTTCTC TTCCAAATCC TCCCTTTGAT GATGAAGTTG CTTTGAACCT CACAAATAGA 
ATAAAGTCAA GGTTGCTTGA GCTCGAAAAG GATTTGCAAG ATAAGAAGAA ATCAAACGAA 
TTCGAACTCA AGACAGCATG GGACCACTAC ATGGATGCAA ATGGCCGTTG GAATAATAAT 
GAGGCTCAAA AAGAAGCTAA AAAAGGCATT AAGAATGGCC TTTTGAAACG CATTAAGGAA 
AAGGAAAATG AACGTGATTC TTTTGAACTT CAAGTTTCAA ATGTTGATCT TTCTCACATT 
GATGAGAAAG AGAAAAACAT GTCGATTGAG GTTGAGAGAA AGAAGAACCA GCTTGCTAGA 
AGAGAATTTG AATCAACAAT AATTCAAAAG GAAAGTGAGC TGTATAGCAT TGGGCAAATG 
ATTAAGGTTG CTGATCGAGA AAAAAGCATC CTGGATCTTG ATTCGGAGGA CAGAGTAAAA 
CTGTCTATAA AGAAGACAGA ATTGGAGAAT CTAAAAAAGA AACATAGAAA GATAATTGAT 
GAATACAAGG ACAGAATTAG AGGAATGTTA AAGGGGAGGC TTCCTCCTGA GAAGGATTTC 
AACAAGGAAA TCACTCAAGT CTTGAGGGCT GCTACCAAGG AGTTTGATGA CTTGAGTGCC 
AAATCTCGCG AAGCAGAGAA GGAGGTGAAT ATGTTGGAAA TGAGAGTACA AGAAGTTAAC 
AATAACCTCT CCAAGCATCG TAAAGATATG GACTCAAAAA TGAGATATAT CGAATCTAAA 
CTTCAGGCCC TAGATCAGCA ATCTTTTACT GCTGATTCCT ATCCCAAAGT TTTGGATTCA 
GCTAAGGAGA AAAGAGATGT TGAGAAAAGG AAATACAACT TTGCAGATGG TATGCGACAA 
ATGTTTGATC CTTTTGAGAG AGTTGCCCGT GCCAATCATA TTTGCCCTTG CTGTGAACGC 
CCCTTCTCTC TCAAAGAGGA AGATGAATTT GTTAAAAAGC AAAGAATGAA TTCTGCAAGT 
TCTGCTGAGA AAATAAAAGT GCTGGCAGCG GAGTCCTTGA GTGCTGATTC GTTTTTTCAG 
CAATTGGACA AGCTCCGCAT GGTTTATGAA GAATATGTTA ACATTGGAAA GGAAACAATC 
CCTAATGCAG AGAAAGAACT ACGTGACCTT ACTGAAGAGA TGGAACAAAA GTCTCAGGCC 
CTCGATGATG TGCTAGCTGT TTCAGCTCAA GTGAAGGCTG ATAAGGACTC AATTCAGGCC 
CTGGTGCAAC CCATTGAAAC TGCTGACAGA CTATTTCAAG AAATACAAAC ATTGCAGAAG 
CAAGTTGATG AGTTGGTGTA TAAGCTTGAA TATCAAGGGC AGGGTGCGAA ATCCCTGAAG 
GATATTGAAT CGGAGCTGAA TGGTCTGCGT AGTAGAGAGG ATAATTTGCG TGATGAACTA 
GAGAAGTTAA GGGAAGAACG AAGGTACATG GAAAATGATT TATCAGACAC TAGAATACGG 
TGGCACAGTC TAAAGGAAGA GAAAGGCAAG GCAGCTAATA TCTTGCGTGA TGTCGAAAGG 
GTAGAAGCAG AGTTGGATCA TTTGACTGAG GAAAAGAGTC AAGTTGATCT TGATGAAAAG 
CATTTGGAAG AGGCTCATGG CCCTTTATCA AGGGAGAAAG ATGAATTACT TCGTGACTAC 
AATAACTTAA AAGTTAAACT TGATCGCGAA TATGAGGAGC AAGCTGAAAA AAAAGGAAAA 
TATCAGCAGG AAGTTGTGGC ACTACTTACG CTTATTTCTA AAATTAAACA GTATAATGAT 
GAAAAGAAAG GAGAGAAGTT GCAGGAACTG CAAGTAAATA AATGTCTATC AGAATCTCAA 
CTTCAAAGTT GTGATACTAG AAAGCAGGAA ATTTCAACTG AATTAGACAA AAGCAAAGAA 
TTAAAGCGGA ATCAAGATCA GTTAAAACGA AATATCGAGG ATAATTTGAA TTACAGGAAA 
ACAAAAGCTG AAGTAGAGGA ACTTTCCCAG GAGATTGAAT CACTGGAAGA CAGAATTCTG 
AAGATTGGTG GGAAATCCAA AATTGAAGCT GAATTTGGAA AGCTCTCACA GGAGAGGGAG 
AGGCTTCTTT CAGAGTTAAA CATTCATCGT GGAACCATGT CTGTTTACCA GAAAAATATT 
TCAAGGGATA TAAGTGATCT CAAACAGGCA CAATACAAGG AAATTGACAA GCGGTACTTT 
GATCAGTTAA TCCAGCTAAA GACAACTGAG ATGGCAAACA AGGACCTGGA CAGATACTAC 
AATGCGCTTG ACAAAGCACT GATGCGCTTC CACACCATGA AAATGGAGGA AATAAATAAA 
ATTATAAGGG AGTTGTGGCA GCAAACATAC AGAGGACAAG ATATAGACTA CATATGTATA 
CATTCAGATT CTGAAGGTGC TGGGACTCGT TCATATAGCT ACAAGGTTCT CATGCAGACC 
GGTGATGCAG AGCTCGAAAT GAGAGGAAGA TGCAGTGCTG GTCAGAAGGT CCTCGCTTCC 
CTTATCATAA GGTTGGCTTT AGCTGAAACT TTCTGCCTCA ACTGTGGAAT ACTAGCACTA 
GATGAACCGA CGACAAATTT GGATGGCCCA AATGCAGACA GTCTTGCTGC TGCTCTCCAT 
AGAATTATGG AGGACCGGAA GGGCCAGGAA AACTTTCAGC TGATTGTAAT TACTCATGAT 
GAGCGTTTTG CTCAACTAAT TGGCCAACGC CAGCATGCAG AGAAATATTA TCGGGTGGCA 
AAGGACGATC ACCAGCATAG CATAATTGAA GCCCAGGAGA TTTTTGATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021834735.1
CDS	193..4143
Translation	MSTVDKMLIKGIRSFDPENKHVITFFKPLTLIVGPNGAGKTTIIECLKLSCSGELPPNARSGHSFIHDPKVAGETETKAQIKLRFRTAAGKDVVCIRSFQLTQKASKMEFKAIDSVLQTINPHTGEKVCLSYRCADMDREIPALMGVSKAILENVIFVHQDEANWPLQDPSTLKKKFDDIFSATRYTKALEVIKKLHKDQAQEIKTYKLKLENLQTLKDVAYKLRESISKDQEKTESVKSQMQELEGSIRDVDAKIRYTEATLKDLRELQDQISMKTAVRSTLFKEQQKKYADLAEENEDTDEELKEWKTKFEERIALLETKISKLEREMEDTETKSGVLKKTLEKSIWEISKLQTEAEVHRSSKSERDSIIQNFFTRHNLGSLPNPPFDDEVALNLTNRIKSRLLELEKDLQDKKKSNEFELKTAWDHYMDANGRWNNNEAQKEAKKGIKNGLLKRIKEKENERDSFELQVSNVDLSHIDEKEKNMSIEVERKKNQLARREFESTIIQKESELYSIGQMIKVADREKSILDLDSEDRVKLSIKKTELENLKKKHRKIIDEYKDRIRGMLKGRLPPEKDFNKEITQVLRAATKEFDDLSAKSREAEKEVNMLEMRVQEVNNNLSKHRKDMDSKMRYIESKLQALDQQSFTADSYPKVLDSAKEKRDVEKRKYNFADGMRQMFDPFERVARANHICPCCERPFSLKEEDEFVKKQRMNSASSAEKIKVLAAESLSADSFFQQLDKLRMVYEEYVNIGKETIPNAEKELRDLTEEMEQKSQALDDVLAVSAQVKADKDSIQALVQPIETADRLFQEIQTLQKQVDELVYKLEYQGQGAKSLKDIESELNGLRSREDNLRDELEKLREERRYMENDLSDTRIRWHSLKEEKGKAANILRDVERVEAELDHLTEEKSQVDLDEKHLEEAHGPLSREKDELLRDYNNLKVKLDREYEEQAEKKGKYQQEVVALLTLISKIKQYNDEKKGEKLQELQVNKCLSESQLQSCDTRKQEISTELDKSKELKRNQDQLKRNIEDNLNYRKTKAEVEELSQEIESLEDRILKIGGKSKIEAEFGKLSQERERLLSELNIHRGTMSVYQKNISRDISDLKQAQYKEIDKRYFDQLIQLKTTEMANKDLDRYYNALDKALMRFHTMKMEEINKIIRELWQQTYRGQDIDYICIHSDSEGAGTRSYSYKVLMQTGDAELEMRGRCSAGQKVLASLIIRLALAETFCLNCGILALDEPTTNLDGPNADSLAAALHRIMEDRKGQENFQLIVITHDERFAQLIGQRQHAEKYYRVAKDDHQHSIIEAQEIFD

Target ORF information:

RefSeq Version	XM_021979043.1
Organism	Prunus avium(sweet cherry)
Definition	Prunus avium DNA repair protein RAD50 (LOC110774500), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021979043.1

ORF Insert Sequence:

ATGAGTACGG TGGACAAGAT GCTGATAAAG GGAATTCGGA GCTTCGATCC GGAGAACAAG 
CACGTCATCA CCTTCTTCAA GCCCTTAACC CTAATCGTCG GCCCAAACGG CGCCGGAAAA 
ACCACCATTA TCGAGTGCTT GAAGCTCTCT TGCTCCGGCG AGTTGCCTCC CAATGCTAGG 
TCTGGCCACA GCTTCATCCA TGACCCGAAA GTTGCCGGCG AAACCGAGAC CAAAGCCCAG 
ATTAAGCTTC GATTCAGAAC CGCAGCGGGT AAAGATGTGG TGTGCATTAG GTCATTTCAG 
CTCACGCAGA AAGCCTCGAA AATGGAATTC AAAGCCATCG ACAGTGTCCT TCAAACCATT 
AATCCCCACA CGGGAGAGAA AGTTTGCCTT AGCTATAGAT GTGCTGATAT GGATAGGGAA 
ATCCCTGCTT TAATGGGTGT GTCAAAGGCT ATTTTGGAAA ATGTTATATT TGTGCACCAA 
GATGAAGCCA ATTGGCCTTT GCAAGATCCT TCCACATTGA AGAAAAAGTT TGATGATATC 
TTCTCTGCTA CCCGATATAC CAAGGCATTG GAGGTGATAA AAAAACTTCA CAAGGATCAA 
GCTCAAGAAA TAAAGACCTA CAAGCTAAAA TTGGAGAATC TTCAGACCTT GAAAGATGTT 
GCATATAAGC TTCGAGAGAG TATTTCTAAA GACCAAGAAA AAACAGAATC TGTGAAAAGT 
CAGATGCAAG AATTGGAGGG AAGCATCAGA GACGTGGATG CTAAAATTCG TTATACTGAA 
GCAACACTGA AAGATTTGCG GGAGCTTCAG GACCAGATAT CGATGAAGAC TGCTGTAAGA 
AGCACTCTCT TTAAGGAGCA GCAGAAAAAG TACGCAGATC TTGCTGAGGA GAACGAAGAC 
ACTGATGAAG AATTGAAGGA ATGGAAAACC AAGTTTGAAG AAAGGATTGC ACTTTTGGAA 
ACTAAAATTA GCAAGTTAGA GAGGGAAATG GAGGACACAG AGACCAAAAG TGGAGTCCTT 
AAGAAGACAC TAGAGAAATC TATATGGGAG ATTAGCAAGC TTCAAACCGA AGCTGAAGTT 
CATAGGTCCT CAAAGAGCGA GAGAGATTCT ATCATTCAAA ACTTTTTTAC AAGGCACAAT 
TTAGGTTCTC TTCCAAATCC TCCCTTTGAT GATGAAGTTG CTTTGAACCT CACAAATAGA 
ATAAAGTCAA GGTTGCTTGA GCTCGAAAAG GATTTGCAAG ATAAGAAGAA ATCAAACGAA 
TTCGAACTCA AGACAGCATG GGACCACTAC ATGGATGCAA ATGGCCGTTG GAATAATAAT 
GAGGCTCAAA AAGAAGCTAA AAAAGGCATT AAGAATGGCC TTTTGAAACG CATTAAGGAA 
AAGGAAAATG AACGTGATTC TTTTGAACTT CAAGTTTCAA ATGTTGATCT TTCTCACATT 
GATGAGAAAG AGAAAAACAT GTCGATTGAG GTTGAGAGAA AGAAGAACCA GCTTGCTAGA 
AGAGAATTTG AATCAACAAT AATTCAAAAG GAAAGTGAGC TGTATAGCAT TGGGCAAATG 
ATTAAGGTTG CTGATCGAGA AAAAAGCATC CTGGATCTTG ATTCGGAGGA CAGAGTAAAA 
CTGTCTATAA AGAAGACAGA ATTGGAGAAT CTAAAAAAGA AACATAGAAA GATAATTGAT 
GAATACAAGG ACAGAATTAG AGGAATGTTA AAGGGGAGGC TTCCTCCTGA GAAGGATTTC 
AACAAGGAAA TCACTCAAGT CTTGAGGGCT GCTACCAAGG AGTTTGATGA CTTGAGTGCC 
AAATCTCGCG AAGCAGAGAA GGAGGTGAAT ATGTTGGAAA TGAGAGTACA AGAAGTTAAC 
AATAACCTCT CCAAGCATCG TAAAGATATG GACTCAAAAA TGAGATATAT CGAATCTAAA 
CTTCAGGCCC TAGATCAGCA ATCTTTTACT GCTGATTCCT ATCCCAAAGT TTTGGATTCA 
GCTAAGGAGA AAAGAGATGT TGAGAAAAGG AAATACAACT TTGCAGATGG TATGCGACAA 
ATGTTTGATC CTTTTGAGAG AGTTGCCCGT GCCAATCATA TTTGCCCTTG CTGTGAACGC 
CCCTTCTCTC TCAAAGAGGA AGATGAATTT GTTAAAAAGC AAAGAATGAA TTCTGCAAGT 
TCTGCTGAGA AAATAAAAGT GCTGGCAGCG GAGTCCTTGA GTGCTGATTC GTTTTTTCAG 
CAATTGGACA AGCTCCGCAT GGTTTATGAA GAATATGTTA ACATTGGAAA GGAAACAATC 
CCTAATGCAG AGAAAGAACT ACGTGACCTT ACTGAAGAGA TGGAACAAAA GTCTCAGGCC 
CTCGATGATG TGCTAGCTGT TTCAGCTCAA GTGAAGGCTG ATAAGGACTC AATTCAGGCC 
CTGGTGCAAC CCATTGAAAC TGCTGACAGA CTATTTCAAG AAATACAAAC ATTGCAGAAG 
CAAGTTGATG AGTTGGTGTA TAAGCTTGAA TATCAAGGGC AGGGTGCGAA ATCCCTGAAG 
GATATTGAAT CGGAGCTGAA TGGTCTGCGT AGTAGAGAGG ATAATTTGCG TGATGAACTA 
GAGAAGTTAA GGGAAGAACG AAGGTACATG GAAAATGATT TATCAGACAC TAGAATACGG 
TGGCACAGTC TAAAGGAAGA GAAAGGCAAG GCAGCTAATA TCTTGCGTGA TGTCGAAAGG 
GTAGAAGCAG AGTTGGATCA TTTGACTGAG GAAAAGAGTC AAGTTGATCT TGATGAAAAG 
CATTTGGAAG AGGCTCATGG CCCTTTATCA AGGGAGAAAG ATGAATTACT TCGTGACTAC 
AATAACTTAA AAGTTAAACT TGATCGCGAA TATGAGGAGC AAGCTGAAAA AAAAGGAAAA 
TATCAGCAGG AAGTTGTGGC ACTACTTACG CTTATTTCTA AAATTAAACA GTATAATGAT 
GAAAAGAAAG GAGAGAAGTT GCAGGAACTG CAAGTAAATA AATGTCTATC AGAATCTCAA 
CTTCAAAGTT GTGATACTAG AAAGCAGGAA ATTTCAACTG AATTAGACAA AAGCAAAGAA 
TTAAAGCGGA ATCAAGATCA GTTAAAACGA AATATCGAGG ATAATTTGAA TTACAGGAAA 
ACAAAAGCTG AAGTAGAGGA ACTTTCCCAG GAGATTGAAT CACTGGAAGA CAGAATTCTG 
AAGATTGGTG GGAAATCCAA AATTGAAGCT GAATTTGGAA AGCTCTCACA GGAGAGGGAG 
AGGCTTCTTT CAGAGTTAAA CATTCATCGT GGAACCATGT CTGTTTACCA GAAAAATATT 
TCAAGGGATA TAAGTGATCT CAAACAGGCA CAATACAAGG AAATTGACAA GCGGTACTTT 
GATCAGTTAA TCCAGCTAAA GACAACTGAG ATGGCAAACA AGGACCTGGA CAGATACTAC 
AATGCGCTTG ACAAAGCACT GATGCGCTTC CACACCATGA AAATGGAGGA AATAAATAAA 
ATTATAAGGG AGTTGTGGCA GCAAACATAC AGAGGACAAG ATATAGACTA CATATGTATA 
CATTCAGATT CTGAAGGTGC TGGGACTCGT TCATATAGCT ACAAGGTTCT CATGCAGACC 
GGTGATGCAG AGCTCGAAAT GAGAGGAAGA TGCAGTGCTG GTCAGAAGGT CCTCGCTTCC 
CTTATCATAA GGTTGGCTTT AGCTGAAACT TTCTGCCTCA ACTGTGGAAT ACTAGCACTA 
GATGAACCGA CGACAAATTT GGATGGCCCA AATGCAGACA GTCTTGCTGC TGCTCTCCAT 
AGAATTATGG AGGACCGGAA GGGCCAGGAA AACTTTCAGC TGATTGTAAT TACTCATGAT 
GAGCGTTTTG CTCAACTAAT TGGCCAACGC CAGCATGCAG AGAAATATTA TCGGGTGGCA 
AAGGACGATC ACCAGCATAG CATAATTGAA GCCCAGGAGA TTTTTGATTG A