RHOT2 cDNA ORF clone, Pogona vitticeps(central bearded dragon)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHOT2
Entrez Gene ID	110089417
Full Name	ras homolog family member T2
Gene Type	protein-coding
Organism	Pogona vitticeps(central bearded dragon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020812481.1	XP_020668140.1	mitochondrial Rho GTPase 2

Pathways from BioSystems
Gene Ontology

The following RHOT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHOT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo256329	XM_020812481.1 Latest version!	Pogona vitticeps ras homolog family member T2 (RHOT2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHOT2 ( XM_020812481.1 ) cDNA ORF clone, Pogona vitticeps(central bearded dragon) -> XP_020668140.1 Pogona vitticeps ras homolog family member T2 (RHOT2), mRNA.

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CloneID	OPo256329
Clone ID Related Accession (Same CDS sequence)	XM_020812481.1
Accession Version	XM_020812481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-09
Organism	Pogona vitticeps(central bearded dragon)
Product	mitochondrial Rho GTPase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018151421.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pogona vitticeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCCCTGG TGGGCGAGGA GTTCCCGGAA GAGGTACCTC CTCGGGCAGA AGAGATCACA 
ATTCCGGCAG ACGTCACCCC CGAAAAAGTC CCTACCCACA TTGTGGACTA CTCAGAATCG 
GAACAGACGG AGGAAGAACT CCAAGAAGAA ATTGCCAAGG CGAACGTGGT GTGTATGGTC 
TATGACGTCA CGGAGGAGTC CACCATCGAT AAGATTCGGA CAAAATGGAT TCCGATGGTG 
AATGGGGGAG CTGACAAGTG TTCCAGGATC CCAATCATCC TTGTGGGAAA CAAGTCGGAC 
CTGCAGACCG GGAGCTCCAT GGAAACCATC CTGCCCATTA TGAACCAGTT CTCAGAAATA 
GAGACCTGTG TGGAGTGTTC CGCCAAGAAT CTGAAGAACA TCTCCGAGCT ATTCTACTAT 
GCCCAGAAGG CGGTTTTGCA CCCGACGGCT CCTCTGTACG ACCCAGAGGA GAAACAGAAG 
TCCTGCTTCG GCAACCCTCT GGCTCCCCAA GCGCTAGAAG ACGTGAAGAT GGTGGTCTGG 
AAGAACACGA CCGACGGGGT TCAGGACAAC GGCCTGACAC TCAATGGCTT CCTTTTCCTC 
AACACCCTCT TCATCCAGAG GGGGAGACAC GAGACCACCT GGACCATCCT GCGCCGCTTC 
GGCTACGACG ATGCCCTGGA GCTGACGGAC GAATATCTCT ACCCGCCGAT CCGGGTGCCC 
CAGGACTGCT CCACGGAGCT CAATCACTTC GCCTACCAGT TCCTCCAGAG GATCTTTGAG 
AAACACGACA AGGACCGGGA CGGCGCCCTC TCCCCAGCCG AGCTGCAGAG CTTCTTCAGC 
GTCTTCCCTT ACGTGCCCTG GGGCACCGAG CTCTACCACA CGGTATGCAC CACTGATAAC 
GGCCTGCTTT CCCTGCATGG ATTCCTCTGC CAGTGGACGC TGGTCGCCTA CTTGGACGTC 
CATCGCTGCC TGGAGCACCT CGGTTACCTG GGCTACCCCA TCTTCTCAGA GCAGGATTCT 
CAGGTTCACG CCATCACAGT TACACGGGAG AAAAGGATTG ACCTGGAGAA GGGCCAGACG 
CATCGGAACG TCTTCCTGTG CAAAGTGATC GGATCTCGAG GCGCTGGCAA GTCTGCCTTC 
CTGCAGGCCT TCCTCGGGAA GAACCTGTCT GCCCAAAGAA GATCTGAAGG GAAGCTGTCC 
TGTTATACAA TTAACACCGT GCAAGTCAAC GGGCAAGAGA AGCATTTAAT ACTGCAAGAG 
GTGGAGGCGG ACGTGGAGTT TGCGAAAGCA TCCGACGCGG CTTGCGATGT TGCCTGCTTA 
TTGTACGACG CGACGGACCA CAAGTCGTTC AACTACTGTG CCAGCGTTTA TAAGCAGCAC 
TACATGGACG GTCAGATTCC ATGCCTCTTT GTGGCCTCTA GAGCAGACCT GCCAGAGACG 
ACATTCCCCC ACGGGCTCTC GCCGGCCGAA TTCTGCTACA AGCATCGCCT CCCTCCGCCG 
TTCTGCTTCA GCTGCAACGG ACAAGGGCCA CCCAACCCCG CCATCTATGC CAAGCTGGCC 
ACAGCGGCTA CCTTTCCGCA CCTCAACGAC ATTGACCTGG GCACCGCCTC TTTCTGGATC 
CGGGTTGCCT TGGGAGCCAC CGTGACCGCC ATTGTGGGCG TCGCGCTCTA CAAGGCGATG 
GCCAAGAACA AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020668140.1
CDS	50..1744
Translation	MALVGEEFPEEVPPRAEEITIPADVTPEKVPTHIVDYSESEQTEEELQEEIAKANVVCMVYDVTEESTIDKIRTKWIPMVNGGADKCSRIPIILVGNKSDLQTGSSMETILPIMNQFSEIETCVECSAKNLKNISELFYYAQKAVLHPTAPLYDPEEKQKSCFGNPLAPQALEDVKMVVWKNTTDGVQDNGLTLNGFLFLNTLFIQRGRHETTWTILRRFGYDDALELTDEYLYPPIRVPQDCSTELNHFAYQFLQRIFEKHDKDRDGALSPAELQSFFSVFPYVPWGTELYHTVCTTDNGLLSLHGFLCQWTLVAYLDVHRCLEHLGYLGYPIFSEQDSQVHAITVTREKRIDLEKGQTHRNVFLCKVIGSRGAGKSAFLQAFLGKNLSAQRRSEGKLSCYTINTVQVNGQEKHLILQEVEADVEFAKASDAACDVACLLYDATDHKSFNYCASVYKQHYMDGQIPCLFVASRADLPETTFPHGLSPAEFCYKHRLPPPFCFSCNGQGPPNPAIYAKLATAATFPHLNDIDLGTASFWIRVALGATVTAIVGVALYKAMAKNK

Target ORF information:

RefSeq Version	XM_020812481.1
Organism	Pogona vitticeps(central bearded dragon)
Definition	Pogona vitticeps ras homolog family member T2 (RHOT2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020812481.1

ORF Insert Sequence:

ATGGCCCTGG TGGGCGAGGA GTTCCCGGAA GAGGTACCTC CTCGGGCAGA AGAGATCACA 
ATTCCGGCAG ACGTCACCCC CGAAAAAGTC CCTACCCACA TTGTGGACTA CTCAGAATCG 
GAACAGACGG AGGAAGAACT CCAAGAAGAA ATTGCCAAGG CGAACGTGGT GTGTATGGTC 
TATGACGTCA CGGAGGAGTC CACCATCGAT AAGATTCGGA CAAAATGGAT TCCGATGGTG 
AATGGGGGAG CTGACAAGTG TTCCAGGATC CCAATCATCC TTGTGGGAAA CAAGTCGGAC 
CTGCAGACCG GGAGCTCCAT GGAAACCATC CTGCCCATTA TGAACCAGTT CTCAGAAATA 
GAGACCTGTG TGGAGTGTTC CGCCAAGAAT CTGAAGAACA TCTCCGAGCT ATTCTACTAT 
GCCCAGAAGG CGGTTTTGCA CCCGACGGCT CCTCTGTACG ACCCAGAGGA GAAACAGAAG 
TCCTGCTTCG GCAACCCTCT GGCTCCCCAA GCGCTAGAAG ACGTGAAGAT GGTGGTCTGG 
AAGAACACGA CCGACGGGGT TCAGGACAAC GGCCTGACAC TCAATGGCTT CCTTTTCCTC 
AACACCCTCT TCATCCAGAG GGGGAGACAC GAGACCACCT GGACCATCCT GCGCCGCTTC 
GGCTACGACG ATGCCCTGGA GCTGACGGAC GAATATCTCT ACCCGCCGAT CCGGGTGCCC 
CAGGACTGCT CCACGGAGCT CAATCACTTC GCCTACCAGT TCCTCCAGAG GATCTTTGAG 
AAACACGACA AGGACCGGGA CGGCGCCCTC TCCCCAGCCG AGCTGCAGAG CTTCTTCAGC 
GTCTTCCCTT ACGTGCCCTG GGGCACCGAG CTCTACCACA CGGTATGCAC CACTGATAAC 
GGCCTGCTTT CCCTGCATGG ATTCCTCTGC CAGTGGACGC TGGTCGCCTA CTTGGACGTC 
CATCGCTGCC TGGAGCACCT CGGTTACCTG GGCTACCCCA TCTTCTCAGA GCAGGATTCT 
CAGGTTCACG CCATCACAGT TACACGGGAG AAAAGGATTG ACCTGGAGAA GGGCCAGACG 
CATCGGAACG TCTTCCTGTG CAAAGTGATC GGATCTCGAG GCGCTGGCAA GTCTGCCTTC 
CTGCAGGCCT TCCTCGGGAA GAACCTGTCT GCCCAAAGAA GATCTGAAGG GAAGCTGTCC 
TGTTATACAA TTAACACCGT GCAAGTCAAC GGGCAAGAGA AGCATTTAAT ACTGCAAGAG 
GTGGAGGCGG ACGTGGAGTT TGCGAAAGCA TCCGACGCGG CTTGCGATGT TGCCTGCTTA 
TTGTACGACG CGACGGACCA CAAGTCGTTC AACTACTGTG CCAGCGTTTA TAAGCAGCAC 
TACATGGACG GTCAGATTCC ATGCCTCTTT GTGGCCTCTA GAGCAGACCT GCCAGAGACG 
ACATTCCCCC ACGGGCTCTC GCCGGCCGAA TTCTGCTACA AGCATCGCCT CCCTCCGCCG 
TTCTGCTTCA GCTGCAACGG ACAAGGGCCA CCCAACCCCG CCATCTATGC CAAGCTGGCC 
ACAGCGGCTA CCTTTCCGCA CCTCAACGAC ATTGACCTGG GCACCGCCTC TTTCTGGATC 
CGGGTTGCCT TGGGAGCCAC CGTGACCGCC ATTGTGGGCG TCGCGCTCTA CAAGGCGATG 
GCCAAGAACA AGTAG