kcmf1 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	kcmf1
Entrez Gene ID	103155136
Full Name	potassium channel modulatory factor 1
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007576686.2	XP_007576748.1	E3 ubiquitin-protein ligase KCMF1

Pathways from BioSystems
Gene Ontology

The following kcmf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kcmf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo158288	XM_007576686.2 Latest version!	Poecilia formosa potassium channel modulatory factor 1 (kcmf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kcmf1 ( XM_007576686.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007576748.1 Poecilia formosa potassium channel modulatory factor 1 (kcmf1), mRNA.

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CloneID	OPo158288
Clone ID Related Accession (Same CDS sequence)	XM_007576686.2
Accession Version	XM_007576686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	E3 ubiquitin-protein ligase KCMF1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800353.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007576686.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGAC ATGAGGGTGT GAGCTGTGAT GCATGTTTAA AGGGCAACTT TAGAGGACGG 
AGGTTTAAGT GTCTAATTTG CTACGACTAC GACCTGTGTG CATCGTGCTA CGAGAGCGGG 
GCCACCACAA CACGACACAC CACAGAGCAC CCCATGCAGT GTATATTAAC CAGAGTAGAC 
TATGATTTGT ATTATGGAGG AGACACCTTT TCGGTGGAAC AACCTCAGTC GTTCACATGT 
CCTTATTGTG GTAAGATGGG CTTCACGGAG ACGTCCTTAC AGGAGCACGT CACCGCAGAG 
CATTCGGAGA CGTCCACAGA GGTGATCTGT CCAATCTGCG CTGCCTTGCC AGGAGGCGAT 
CCGAACCACG TGACGGACGA CTTTACGGCT CACCTCACCC TTGAGCACAG AGCGCCAAGA 
GATTTAGACG AGTCCAGTAG CGTTCGCCAC GTACGCAGGA TGTTCCACCC AGGGCGAGGA 
ATCGGCGGCC CCAGAGCTCG ACGGACAAAT ATGCACTTTA CTAGCGGCTC CACAGGGGGG 
CTCTCATCCT CCTCATCACA GAGCTCCACT TACACCCCCA GTAACAGAGA GACAATGGAC 
CCAATCGCAG AGTTGTTGTC TCAGCTGTCC GGCGTGCGGC GGGCTGCTGG GGGCCAGATA 
AACTCGTCGG GCCCCTCGGC CTCCCAGCTG CAGCAGCTCC AGATGCAGCT GCAGCTGGAG 
AGACAGCAGG CGCAGGCAGC CCGGCAGCAG GTAGAGGCAG GCCGCCACGC AACTCGACGT 
GGCAACAACC CCGGCAACAC AGGCAACTCT ATCCCCCCGC CTAACTCGTC CAACGCCAAC 
ACCAGCGCCG TGGGCGATAG CAATCCCGCT TCCTCCTCCC AAAGCTCCCA GTTTCTATTA 
GCACGCTTGA ATGAACCCAA GATGTCCGAA GCGGAGCGCC AGTTCCTAGA AGGAGAGCGA 
GCCGACCGCA GCTTGTTCGT CCAGGAGCTG CTGCTGTCGA CACTGATGCA CGAGGAGAGC 
TCCTCCTCCG ATGAGGACGA GCGCCGAGAC TTCGCCGACT TCGGAGCCAT GGGCTGCGTG 
GACATCATGC CTTTAGACGT GGCGCTGGAG AACCTCCAGC TCCGAGAGAG CAGCTCTTCG 
GGGAGGGAGC CTCCGCCGCC TCCTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007576748.1
CDS	188..1357
Translation	MSRHEGVSCDACLKGNFRGRRFKCLICYDYDLCASCYESGATTTRHTTEHPMQCILTRVDYDLYYGGDTFSVEQPQSFTCPYCGKMGFTETSLQEHVTAEHSETSTEVICPICAALPGGDPNHVTDDFTAHLTLEHRAPRDLDESSSVRHVRRMFHPGRGIGGPRARRTNMHFTSGSTGGLSSSSSQSSTYTPSNRETMDPIAELLSQLSGVRRAAGGQINSSGPSASQLQQLQMQLQLERQQAQAARQQVEAGRHATRRGNNPGNTGNSIPPPNSSNANTSAVGDSNPASSSQSSQFLLARLNEPKMSEAERQFLEGERADRSLFVQELLLSTLMHEESSSSDEDERRDFADFGAMGCVDIMPLDVALENLQLRESSSSGREPPPPPL

Target ORF information:

RefSeq Version	XM_007576686.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa potassium channel modulatory factor 1 (kcmf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007576686.2

ORF Insert Sequence:

ATGTCCCGAC ATGAGGGTGT GAGCTGTGAT GCATGTTTAA AGGGCAACTT TAGAGGACGG 
AGGTTTAAGT GTCTAATTTG CTACGACTAC GACCTGTGTG CATCGTGCTA CGAGAGCGGG 
GCCACCACAA CACGACACAC CACAGAGCAC CCCATGCAGT GTATATTAAC CAGAGTAGAC 
TATGATTTGT ATTATGGAGG AGACACCTTT TCGGTGGAAC AACCTCAGTC GTTCACATGT 
CCTTATTGTG GTAAGATGGG CTTCACGGAG ACGTCCTTAC AGGAGCACGT CACCGCAGAG 
CATTCGGAGA CGTCCACAGA GGTGATCTGT CCAATCTGCG CTGCCTTGCC AGGAGGCGAT 
CCGAACCACG TGACGGACGA CTTTACGGCT CACCTCACCC TTGAGCACAG AGCGCCAAGA 
GATTTAGACG AGTCCAGTAG CGTTCGCCAC GTACGCAGGA TGTTCCACCC AGGGCGAGGA 
ATCGGCGGCC CCAGAGCTCG ACGGACAAAT ATGCACTTTA CTAGCGGCTC CACAGGGGGG 
CTCTCATCCT CCTCATCACA GAGCTCCACT TACACCCCCA GTAACAGAGA GACAATGGAC 
CCAATCGCAG AGTTGTTGTC TCAGCTGTCC GGCGTGCGGC GGGCTGCTGG GGGCCAGATA 
AACTCGTCGG GCCCCTCGGC CTCCCAGCTG CAGCAGCTCC AGATGCAGCT GCAGCTGGAG 
AGACAGCAGG CGCAGGCAGC CCGGCAGCAG GTAGAGGCAG GCCGCCACGC AACTCGACGT 
GGCAACAACC CCGGCAACAC AGGCAACTCT ATCCCCCCGC CTAACTCGTC CAACGCCAAC 
ACCAGCGCCG TGGGCGATAG CAATCCCGCT TCCTCCTCCC AAAGCTCCCA GTTTCTATTA 
GCACGCTTGA ATGAACCCAA GATGTCCGAA GCGGAGCGCC AGTTCCTAGA AGGAGAGCGA 
GCCGACCGCA GCTTGTTCGT CCAGGAGCTG CTGCTGTCGA CACTGATGCA CGAGGAGAGC 
TCCTCCTCCG ATGAGGACGA GCGCCGAGAC TTCGCCGACT TCGGAGCCAT GGGCTGCGTG 
GACATCATGC CTTTAGACGT GGCGCTGGAG AACCTCCAGC TCCGAGAGAG CAGCTCTTCG 
GGGAGGGAGC CTCCGCCGCC TCCTCTTTGA