UBE4A cDNA ORF clone, Phascolarctos cinereus(koala)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE4A
Entrez Gene ID	110207188
Full Name	ubiquitination factor E4A
Gene Type	protein-coding
Organism	Phascolarctos cinereus(koala)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020984778.1	XP_020840437.1	ubiquitin conjugation factor E4 A isoform X1
XM_020984779.1	XP_020840438.1	ubiquitin conjugation factor E4 A isoform X2

Pathways from BioSystems
Gene Ontology

The following UBE4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPh253686	XM_020984778.1 Latest version!	Phascolarctos cinereus ubiquitination factor E4A (UBE4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPh253687	XM_020984779.1 Latest version!	Phascolarctos cinereus ubiquitination factor E4A (UBE4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE4A ( XM_020984778.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020840437.1 Phascolarctos cinereus ubiquitination factor E4A (UBE4A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPh253686
Clone ID Related Accession (Same CDS sequence)	XM_020984778.1
Accession Version	XM_020984778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin conjugation factor E4 A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343970.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACC AGGACAATAA CAACAACATC TCCAGTAACC CCTTTGCTGC TCTCTTCAGT 
TCCCTGGCTG ATGCCAAGCA CTTTGCAGCG ATCCAGAAGG AACAGCTGAA GCAGCAATCT 
GAGAAATGTA TGTTTACAAG ATCAAATTTT CTGGCAGATG AACCCTCAGC TAGCCCTGAT 
GACTCGGATA ACAGTGTGTC GGAAAGTTTG GATGACTGTG ACTATTCTGT CTCTGAGATT 
AGCCGATCAT TCCGTTCCCA GCAGGAGATA TGTGAGCAGC TCAACATCAA TCACATGATC 
CAAAGGATTT TCCTCATCAC CCTGGACAAC AGTGACCCAA GCATGAAAGG TGGTAATGGG 
ATCCCCAGCC GCTGTGTGTA TTTGGAGGAA CTGGCAGGAG ACTTGGACGA GCAAGACTGG 
CTTGATATGA ACAACATTGA GCAGGCCCTC TTTACCCGAT TGCTGCTTCA GGATCCAAGC 
AACCACTTGA TTAACATGAC CTGCTCCACA ACATTAAACC TCTCTGCAGA TCGAGATGCA 
GGGGAGAAGC ACATTCTTTG CTACCTTTAT TCTTGTTTCT TAAGAGCAAA AGAGGAGATT 
ACCAAAGTAC CAGAAAACCT GCTGCCCTTT GCTGTGAGGT GTAGGAACCT AACTGTGTCT 
AATACCCGAA CTGTCCTCCT CACCCCAGAG ATTTATGTTG ACCAGAATGT CTATGATCAG 
CTGGTAGACC TCTTGTTGGA AGCAATTCGA GGGGCCCATT TTGAAGATGT AACCGAGTTC 
CTTGAAGAGG TCATTGAAGC CTTGACAGTG GATCAGGAAG TTCGGACATT TCAAGAAGTC 
ATGGTTCCAG TATTTGACAT TTTGTTGAGC AGAATAAAAG ACTTGGATCT CTGTCAAATC 
TTGCTATACA CATATCTAGA TATGCTTCTG TATTTCACAA GGCAGAAGGA TATTGCGAAA 
GTTTTTGTAG AGTACATTCA ACCCCAGGAC CCCAGCAATG GACAGATGTA CCAGAAGACT 
TTACTGGGCG TAATTCTGAG TATCTCCTGC TTGTTAAAGA CTCCAGGGGT AGTGGAGAAT 
CACAGCTACT TCTTGAATCC ATCCCGATCC AGTCCCCAGG AGATCAAAGT GCAGGAGGCC 
AACATCCATC AGTTCATGGC TCAGTTCCAC GAAAAGATTT ACCAGATGCT GAAGAACTTG 
CTCCAGCTCT CTCCAGACAC AAAGCATTGG ATCTTGTCCT GGCTTGGAAA TTGCCTATAT 
GCCAACACAG GTCGCACCAA GATCTGGGCC AATCAGATGC CAGAAATCTT CTTCCAGATG 
TATGCCTCAG ATGCCTTCTT CCTGAATCTG GGTGCTGCCC TCCTGAAGCT GTGCCAGCCA 
TTTTGCAAAC CTAAATCCCC TAGGCTGCTA ACCTTTAACC CTACCTACTG TGCCCTCAAA 
GAGTTGAATG ATGAAGAGAG AAGGATAAAG AATGTGCATA TGAAAGGTTT GGATAAAGAG 
ACCTGTTTGA TCCCTAAAGA GAGTGACCAG GAGATGCAGT TTGCCCAAAA CTATAACCTG 
GTAACAGAGA ACCTGGTCCT GACACAGTAC ACCTTACACG TGGGATTTCA CAGGTTGCAT 
GACCAGATGG TAAAAATCAA CCAGAGCTTG CACCGGCTGC AGGTCGCTTG GCGTGAGGCT 
CAACAAAGTG CCAGCCCCAC CGCAGACAGC CTTCGAGAGC AGTTTGAGCG CTTGATGACG 
ATCTATCTCT CCACCAAGAC TGCCATGACT GAGCCGCAGA TGCTACAAAA CTGCCTCAAT 
TTGCAGGTGT CCATGGCCGT TCTTCTGGTT CAGCTAGCCA ATGGCAACCA AGGCACAGAG 
CCAGTAGAGC TGACCTTCCC TTTGTCAGAT CAGTACAGCT CTTTGGCTTA TGTGCCAGAA 
TTTTTTGCGG ATAACCTGGG AGATTTTCTT ATTTTCCTAC GGCGCTTTGC TGACGACATC 
CTGGAGACAT CAGCTGATTC ACTGGAGCAA GTTCTTCATT TTGTCACTAT TTTCACTGGG 
AGTGTAGAGA GAATGAAAAA CCCCCACCTG AGGGCCAAAC TGGCAGAGGT GCTGGAAGCA 
GTCATGCCAC ACCTGGACCA AACCCCAAAT CCCCTGGTGT CCAGCGTGTT CCACCGTAAG 
CGTGTGTTCT GCGGCTATCC CTATGCAGCC CAGCTTGCTG AGGCACTCAT CAAAGTCTTT 
GTAGACATCG AGTTTACAGG AGATCCCCAT CAGTTTGAGC AAAAGTTTAA CTACCGCCGC 
CCCATGTATC CTATCTTAAA GTACATGTGG GAAACAGACA CCTACAGAGA AAGCATAAAG 
GCTCTAGCTG ATTATGCCTC TGCAAATTTG GAGGCCATGA ATCCCCCTCT CTTTCTCCGC 
TTCCTTAACC TGCTGATGAA TGATGCTATC TTCCTACTGG ATGAAGCCAT ACAGTACTTG 
AGCAAGATAA AGATTCAGCA GATTGAGAGG GACCAAGGGG AATGGGACAA TTTGTCTCCA 
GAGGCCCGCC GAGAAAAGGA GGCCAGTCTG CAAATGTTTG GGCAACTGGC TCGATTCCAC 
AACATCATGT CAAATGAAAC CATTGGTACC TTGGCCTTTC TAACATCAGA GATCAAGTCT 
CTCTTTGTAC ATCCCTTCCT GGCTGAACGA ATCATCTCTA TGTTAAACTA CTTCCTACAG 
CACTTGGTTG GTCCTAAGAT GGGAGCCTTA AAAGTCAAGG ACTTCAGCGA GTTTGACTTC 
AAACCCCAGC AGCTTGTTTC AGACATCTGC AACATCTACT TAAACCTTGG TGATGAAGAG 
AATTTCTGTG CCACTGTGCC CAAGGATGGG CGATCTTATT CCCCAATGCT CTTTGCTCAG 
ACAGTCCGAG TCCTGAAGAA AATAAACAAG CCTGGAAATA TGATTGTGGC TTTCAGCAAT 
CTGGCAGAGA AGATCAAGTC TCTTGCAGAT CTCCAGCAAC AGGAGGAAGA GACCTATGCA 
GATGCCTGTG ATGAGTTTCT AGATCCCATC ATGAGTACAC TGATGTCAGA CCCTGTGATG 
CTGCCATCTT CCAGAGTCAC TGTGGATAGG TCCACAATAG CCAGGCATCT GCTCAGTGAT 
CAGACTGATC CCTTTAACCG TAGCCCCCTA ACTATGGACC AGATCCGGCC AAACACAGAA 
CTGAAAGAGA AAATCCAACG GTGGCTCGCT GAGAGGAAAT TACAGAAGGA ACAACTTGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020840437.1
CDS	143..3385
Translation	MTDQDNNNNISSNPFAALFSSLADAKHFAAIQKEQLKQQSEKCMFTRSNFLADEPSASPDDSDNSVSESLDDCDYSVSEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSMKGGNGIPSRCVYLEELAGDLDEQDWLDMNNIEQALFTRLLLQDPSNHLINMTCSTTLNLSADRDAGEKHILCYLYSCFLRAKEEITKVPENLLPFAVRCRNLTVSNTRTVLLTPEIYVDQNVYDQLVDLLLEAIRGAHFEDVTEFLEEVIEALTVDQEVRTFQEVMVPVFDILLSRIKDLDLCQILLYTYLDMLLYFTRQKDIAKVFVEYIQPQDPSNGQMYQKTLLGVILSISCLLKTPGVVENHSYFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPDTKHWILSWLGNCLYANTGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPKSPRLLTFNPTYCALKELNDEERRIKNVHMKGLDKETCLIPKESDQEMQFAQNYNLVTENLVLTQYTLHVGFHRLHDQMVKINQSLHRLQVAWREAQQSASPTADSLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLANGNQGTEPVELTFPLSDQYSSLAYVPEFFADNLGDFLIFLRRFADDILETSADSLEQVLHFVTIFTGSVERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCGYPYAAQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILKYMWETDTYRESIKALADYASANLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIERDQGEWDNLSPEARREKEASLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICNIYLNLGDEENFCATVPKDGRSYSPMLFAQTVRVLKKINKPGNMIVAFSNLAEKIKSLADLQQQEEETYADACDEFLDPIMSTLMSDPVMLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKLQKEQLE

Target ORF information:

RefSeq Version	XM_020984778.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitination factor E4A (UBE4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020984778.1

ORF Insert Sequence:

ATGACAGACC AGGACAATAA CAACAACATC TCCAGTAACC CCTTTGCTGC TCTCTTCAGT 
TCCCTGGCTG ATGCCAAGCA CTTTGCAGCG ATCCAGAAGG AACAGCTGAA GCAGCAATCT 
GAGAAATGTA TGTTTACAAG ATCAAATTTT CTGGCAGATG AACCCTCAGC TAGCCCTGAT 
GACTCGGATA ACAGTGTGTC GGAAAGTTTG GATGACTGTG ACTATTCTGT CTCTGAGATT 
AGCCGATCAT TCCGTTCCCA GCAGGAGATA TGTGAGCAGC TCAACATCAA TCACATGATC 
CAAAGGATTT TCCTCATCAC CCTGGACAAC AGTGACCCAA GCATGAAAGG TGGTAATGGG 
ATCCCCAGCC GCTGTGTGTA TTTGGAGGAA CTGGCAGGAG ACTTGGACGA GCAAGACTGG 
CTTGATATGA ACAACATTGA GCAGGCCCTC TTTACCCGAT TGCTGCTTCA GGATCCAAGC 
AACCACTTGA TTAACATGAC CTGCTCCACA ACATTAAACC TCTCTGCAGA TCGAGATGCA 
GGGGAGAAGC ACATTCTTTG CTACCTTTAT TCTTGTTTCT TAAGAGCAAA AGAGGAGATT 
ACCAAAGTAC CAGAAAACCT GCTGCCCTTT GCTGTGAGGT GTAGGAACCT AACTGTGTCT 
AATACCCGAA CTGTCCTCCT CACCCCAGAG ATTTATGTTG ACCAGAATGT CTATGATCAG 
CTGGTAGACC TCTTGTTGGA AGCAATTCGA GGGGCCCATT TTGAAGATGT AACCGAGTTC 
CTTGAAGAGG TCATTGAAGC CTTGACAGTG GATCAGGAAG TTCGGACATT TCAAGAAGTC 
ATGGTTCCAG TATTTGACAT TTTGTTGAGC AGAATAAAAG ACTTGGATCT CTGTCAAATC 
TTGCTATACA CATATCTAGA TATGCTTCTG TATTTCACAA GGCAGAAGGA TATTGCGAAA 
GTTTTTGTAG AGTACATTCA ACCCCAGGAC CCCAGCAATG GACAGATGTA CCAGAAGACT 
TTACTGGGCG TAATTCTGAG TATCTCCTGC TTGTTAAAGA CTCCAGGGGT AGTGGAGAAT 
CACAGCTACT TCTTGAATCC ATCCCGATCC AGTCCCCAGG AGATCAAAGT GCAGGAGGCC 
AACATCCATC AGTTCATGGC TCAGTTCCAC GAAAAGATTT ACCAGATGCT GAAGAACTTG 
CTCCAGCTCT CTCCAGACAC AAAGCATTGG ATCTTGTCCT GGCTTGGAAA TTGCCTATAT 
GCCAACACAG GTCGCACCAA GATCTGGGCC AATCAGATGC CAGAAATCTT CTTCCAGATG 
TATGCCTCAG ATGCCTTCTT CCTGAATCTG GGTGCTGCCC TCCTGAAGCT GTGCCAGCCA 
TTTTGCAAAC CTAAATCCCC TAGGCTGCTA ACCTTTAACC CTACCTACTG TGCCCTCAAA 
GAGTTGAATG ATGAAGAGAG AAGGATAAAG AATGTGCATA TGAAAGGTTT GGATAAAGAG 
ACCTGTTTGA TCCCTAAAGA GAGTGACCAG GAGATGCAGT TTGCCCAAAA CTATAACCTG 
GTAACAGAGA ACCTGGTCCT GACACAGTAC ACCTTACACG TGGGATTTCA CAGGTTGCAT 
GACCAGATGG TAAAAATCAA CCAGAGCTTG CACCGGCTGC AGGTCGCTTG GCGTGAGGCT 
CAACAAAGTG CCAGCCCCAC CGCAGACAGC CTTCGAGAGC AGTTTGAGCG CTTGATGACG 
ATCTATCTCT CCACCAAGAC TGCCATGACT GAGCCGCAGA TGCTACAAAA CTGCCTCAAT 
TTGCAGGTGT CCATGGCCGT TCTTCTGGTT CAGCTAGCCA ATGGCAACCA AGGCACAGAG 
CCAGTAGAGC TGACCTTCCC TTTGTCAGAT CAGTACAGCT CTTTGGCTTA TGTGCCAGAA 
TTTTTTGCGG ATAACCTGGG AGATTTTCTT ATTTTCCTAC GGCGCTTTGC TGACGACATC 
CTGGAGACAT CAGCTGATTC ACTGGAGCAA GTTCTTCATT TTGTCACTAT TTTCACTGGG 
AGTGTAGAGA GAATGAAAAA CCCCCACCTG AGGGCCAAAC TGGCAGAGGT GCTGGAAGCA 
GTCATGCCAC ACCTGGACCA AACCCCAAAT CCCCTGGTGT CCAGCGTGTT CCACCGTAAG 
CGTGTGTTCT GCGGCTATCC CTATGCAGCC CAGCTTGCTG AGGCACTCAT CAAAGTCTTT 
GTAGACATCG AGTTTACAGG AGATCCCCAT CAGTTTGAGC AAAAGTTTAA CTACCGCCGC 
CCCATGTATC CTATCTTAAA GTACATGTGG GAAACAGACA CCTACAGAGA AAGCATAAAG 
GCTCTAGCTG ATTATGCCTC TGCAAATTTG GAGGCCATGA ATCCCCCTCT CTTTCTCCGC 
TTCCTTAACC TGCTGATGAA TGATGCTATC TTCCTACTGG ATGAAGCCAT ACAGTACTTG 
AGCAAGATAA AGATTCAGCA GATTGAGAGG GACCAAGGGG AATGGGACAA TTTGTCTCCA 
GAGGCCCGCC GAGAAAAGGA GGCCAGTCTG CAAATGTTTG GGCAACTGGC TCGATTCCAC 
AACATCATGT CAAATGAAAC CATTGGTACC TTGGCCTTTC TAACATCAGA GATCAAGTCT 
CTCTTTGTAC ATCCCTTCCT GGCTGAACGA ATCATCTCTA TGTTAAACTA CTTCCTACAG 
CACTTGGTTG GTCCTAAGAT GGGAGCCTTA AAAGTCAAGG ACTTCAGCGA GTTTGACTTC 
AAACCCCAGC AGCTTGTTTC AGACATCTGC AACATCTACT TAAACCTTGG TGATGAAGAG 
AATTTCTGTG CCACTGTGCC CAAGGATGGG CGATCTTATT CCCCAATGCT CTTTGCTCAG 
ACAGTCCGAG TCCTGAAGAA AATAAACAAG CCTGGAAATA TGATTGTGGC TTTCAGCAAT 
CTGGCAGAGA AGATCAAGTC TCTTGCAGAT CTCCAGCAAC AGGAGGAAGA GACCTATGCA 
GATGCCTGTG ATGAGTTTCT AGATCCCATC ATGAGTACAC TGATGTCAGA CCCTGTGATG 
CTGCCATCTT CCAGAGTCAC TGTGGATAGG TCCACAATAG CCAGGCATCT GCTCAGTGAT 
CAGACTGATC CCTTTAACCG TAGCCCCCTA ACTATGGACC AGATCCGGCC AAACACAGAA 
CTGAAAGAGA AAATCCAACG GTGGCTCGCT GAGAGGAAAT TACAGAAGGA ACAACTTGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4A ( XM_020984779.1 ) cDNA ORF clone, Phascolarctos cinereus(koala) -> XP_020840438.1 Phascolarctos cinereus ubiquitination factor E4A (UBE4A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPh253687
Clone ID Related Accession (Same CDS sequence)	XM_020984779.1
Accession Version	XM_020984779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-09
Organism	Phascolarctos cinereus(koala)
Product	ubiquitin conjugation factor E4 A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018343970.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Phascolarctos cinereus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGACC AGGACAATAA CAACAACATC TCCAGTAACC CCTTTGCTGC TCTCTTCAGT 
TCCCTGGCTG ATGCCAAGCA CTTTGCAGCG ATCCAGAAGG AACAGCTGAA GCAGCAATCT 
GATGAACCCT CAGCTAGCCC TGATGACTCG GATAACAGTG TGTCGGAAAG TTTGGATGAC 
TGTGACTATT CTGTCTCTGA GATTAGCCGA TCATTCCGTT CCCAGCAGGA GATATGTGAG 
CAGCTCAACA TCAATCACAT GATCCAAAGG ATTTTCCTCA TCACCCTGGA CAACAGTGAC 
CCAAGCATGA AAGGTGGTAA TGGGATCCCC AGCCGCTGTG TGTATTTGGA GGAACTGGCA 
GGAGACTTGG ACGAGCAAGA CTGGCTTGAT ATGAACAACA TTGAGCAGGC CCTCTTTACC 
CGATTGCTGC TTCAGGATCC AAGCAACCAC TTGATTAACA TGACCTGCTC CACAACATTA 
AACCTCTCTG CAGATCGAGA TGCAGGGGAG AAGCACATTC TTTGCTACCT TTATTCTTGT 
TTCTTAAGAG CAAAAGAGGA GATTACCAAA GTACCAGAAA ACCTGCTGCC CTTTGCTGTG 
AGGTGTAGGA ACCTAACTGT GTCTAATACC CGAACTGTCC TCCTCACCCC AGAGATTTAT 
GTTGACCAGA ATGTCTATGA TCAGCTGGTA GACCTCTTGT TGGAAGCAAT TCGAGGGGCC 
CATTTTGAAG ATGTAACCGA GTTCCTTGAA GAGGTCATTG AAGCCTTGAC AGTGGATCAG 
GAAGTTCGGA CATTTCAAGA AGTCATGGTT CCAGTATTTG ACATTTTGTT GAGCAGAATA 
AAAGACTTGG ATCTCTGTCA AATCTTGCTA TACACATATC TAGATATGCT TCTGTATTTC 
ACAAGGCAGA AGGATATTGC GAAAGTTTTT GTAGAGTACA TTCAACCCCA GGACCCCAGC 
AATGGACAGA TGTACCAGAA GACTTTACTG GGCGTAATTC TGAGTATCTC CTGCTTGTTA 
AAGACTCCAG GGGTAGTGGA GAATCACAGC TACTTCTTGA ATCCATCCCG ATCCAGTCCC 
CAGGAGATCA AAGTGCAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCACGAAAAG 
ATTTACCAGA TGCTGAAGAA CTTGCTCCAG CTCTCTCCAG ACACAAAGCA TTGGATCTTG 
TCCTGGCTTG GAAATTGCCT ATATGCCAAC ACAGGTCGCA CCAAGATCTG GGCCAATCAG 
ATGCCAGAAA TCTTCTTCCA GATGTATGCC TCAGATGCCT TCTTCCTGAA TCTGGGTGCT 
GCCCTCCTGA AGCTGTGCCA GCCATTTTGC AAACCTAAAT CCCCTAGGCT GCTAACCTTT 
AACCCTACCT ACTGTGCCCT CAAAGAGTTG AATGATGAAG AGAGAAGGAT AAAGAATGTG 
CATATGAAAG GTTTGGATAA AGAGACCTGT TTGATCCCTA AAGAGAGTGA CCAGGAGATG 
CAGTTTGCCC AAAACTATAA CCTGGTAACA GAGAACCTGG TCCTGACACA GTACACCTTA 
CACGTGGGAT TTCACAGGTT GCATGACCAG ATGGTAAAAA TCAACCAGAG CTTGCACCGG 
CTGCAGGTCG CTTGGCGTGA GGCTCAACAA AGTGCCAGCC CCACCGCAGA CAGCCTTCGA 
GAGCAGTTTG AGCGCTTGAT GACGATCTAT CTCTCCACCA AGACTGCCAT GACTGAGCCG 
CAGATGCTAC AAAACTGCCT CAATTTGCAG GTGTCCATGG CCGTTCTTCT GGTTCAGCTA 
GCCAATGGCA ACCAAGGCAC AGAGCCAGTA GAGCTGACCT TCCCTTTGTC AGATCAGTAC 
AGCTCTTTGG CTTATGTGCC AGAATTTTTT GCGGATAACC TGGGAGATTT TCTTATTTTC 
CTACGGCGCT TTGCTGACGA CATCCTGGAG ACATCAGCTG ATTCACTGGA GCAAGTTCTT 
CATTTTGTCA CTATTTTCAC TGGGAGTGTA GAGAGAATGA AAAACCCCCA CCTGAGGGCC 
AAACTGGCAG AGGTGCTGGA AGCAGTCATG CCACACCTGG ACCAAACCCC AAATCCCCTG 
GTGTCCAGCG TGTTCCACCG TAAGCGTGTG TTCTGCGGCT ATCCCTATGC AGCCCAGCTT 
GCTGAGGCAC TCATCAAAGT CTTTGTAGAC ATCGAGTTTA CAGGAGATCC CCATCAGTTT 
GAGCAAAAGT TTAACTACCG CCGCCCCATG TATCCTATCT TAAAGTACAT GTGGGAAACA 
GACACCTACA GAGAAAGCAT AAAGGCTCTA GCTGATTATG CCTCTGCAAA TTTGGAGGCC 
ATGAATCCCC CTCTCTTTCT CCGCTTCCTT AACCTGCTGA TGAATGATGC TATCTTCCTA 
CTGGATGAAG CCATACAGTA CTTGAGCAAG ATAAAGATTC AGCAGATTGA GAGGGACCAA 
GGGGAATGGG ACAATTTGTC TCCAGAGGCC CGCCGAGAAA AGGAGGCCAG TCTGCAAATG 
TTTGGGCAAC TGGCTCGATT CCACAACATC ATGTCAAATG AAACCATTGG TACCTTGGCC 
TTTCTAACAT CAGAGATCAA GTCTCTCTTT GTACATCCCT TCCTGGCTGA ACGAATCATC 
TCTATGTTAA ACTACTTCCT ACAGCACTTG GTTGGTCCTA AGATGGGAGC CTTAAAAGTC 
AAGGACTTCA GCGAGTTTGA CTTCAAACCC CAGCAGCTTG TTTCAGACAT CTGCAACATC 
TACTTAAACC TTGGTGATGA AGAGAATTTC TGTGCCACTG TGCCCAAGGA TGGGCGATCT 
TATTCCCCAA TGCTCTTTGC TCAGACAGTC CGAGTCCTGA AGAAAATAAA CAAGCCTGGA 
AATATGATTG TGGCTTTCAG CAATCTGGCA GAGAAGATCA AGTCTCTTGC AGATCTCCAG 
CAACAGGAGG AAGAGACCTA TGCAGATGCC TGTGATGAGT TTCTAGATCC CATCATGAGT 
ACACTGATGT CAGACCCTGT GATGCTGCCA TCTTCCAGAG TCACTGTGGA TAGGTCCACA 
ATAGCCAGGC ATCTGCTCAG TGATCAGACT GATCCCTTTA ACCGTAGCCC CCTAACTATG 
GACCAGATCC GGCCAAACAC AGAACTGAAA GAGAAAATCC AACGGTGGCT CGCTGAGAGG 
AAATTACAGA AGGAACAACT TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020840438.1
CDS	143..3349
Translation	MTDQDNNNNISSNPFAALFSSLADAKHFAAIQKEQLKQQSDEPSASPDDSDNSVSESLDDCDYSVSEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSMKGGNGIPSRCVYLEELAGDLDEQDWLDMNNIEQALFTRLLLQDPSNHLINMTCSTTLNLSADRDAGEKHILCYLYSCFLRAKEEITKVPENLLPFAVRCRNLTVSNTRTVLLTPEIYVDQNVYDQLVDLLLEAIRGAHFEDVTEFLEEVIEALTVDQEVRTFQEVMVPVFDILLSRIKDLDLCQILLYTYLDMLLYFTRQKDIAKVFVEYIQPQDPSNGQMYQKTLLGVILSISCLLKTPGVVENHSYFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPDTKHWILSWLGNCLYANTGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPKSPRLLTFNPTYCALKELNDEERRIKNVHMKGLDKETCLIPKESDQEMQFAQNYNLVTENLVLTQYTLHVGFHRLHDQMVKINQSLHRLQVAWREAQQSASPTADSLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLANGNQGTEPVELTFPLSDQYSSLAYVPEFFADNLGDFLIFLRRFADDILETSADSLEQVLHFVTIFTGSVERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCGYPYAAQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILKYMWETDTYRESIKALADYASANLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIERDQGEWDNLSPEARREKEASLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICNIYLNLGDEENFCATVPKDGRSYSPMLFAQTVRVLKKINKPGNMIVAFSNLAEKIKSLADLQQQEEETYADACDEFLDPIMSTLMSDPVMLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKLQKEQLE

Target ORF information:

RefSeq Version	XM_020984779.1
Organism	Phascolarctos cinereus(koala)
Definition	Phascolarctos cinereus ubiquitination factor E4A (UBE4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020984779.1

ORF Insert Sequence:

ATGACAGACC AGGACAATAA CAACAACATC TCCAGTAACC CCTTTGCTGC TCTCTTCAGT 
TCCCTGGCTG ATGCCAAGCA CTTTGCAGCG ATCCAGAAGG AACAGCTGAA GCAGCAATCT 
GATGAACCCT CAGCTAGCCC TGATGACTCG GATAACAGTG TGTCGGAAAG TTTGGATGAC 
TGTGACTATT CTGTCTCTGA GATTAGCCGA TCATTCCGTT CCCAGCAGGA GATATGTGAG 
CAGCTCAACA TCAATCACAT GATCCAAAGG ATTTTCCTCA TCACCCTGGA CAACAGTGAC 
CCAAGCATGA AAGGTGGTAA TGGGATCCCC AGCCGCTGTG TGTATTTGGA GGAACTGGCA 
GGAGACTTGG ACGAGCAAGA CTGGCTTGAT ATGAACAACA TTGAGCAGGC CCTCTTTACC 
CGATTGCTGC TTCAGGATCC AAGCAACCAC TTGATTAACA TGACCTGCTC CACAACATTA 
AACCTCTCTG CAGATCGAGA TGCAGGGGAG AAGCACATTC TTTGCTACCT TTATTCTTGT 
TTCTTAAGAG CAAAAGAGGA GATTACCAAA GTACCAGAAA ACCTGCTGCC CTTTGCTGTG 
AGGTGTAGGA ACCTAACTGT GTCTAATACC CGAACTGTCC TCCTCACCCC AGAGATTTAT 
GTTGACCAGA ATGTCTATGA TCAGCTGGTA GACCTCTTGT TGGAAGCAAT TCGAGGGGCC 
CATTTTGAAG ATGTAACCGA GTTCCTTGAA GAGGTCATTG AAGCCTTGAC AGTGGATCAG 
GAAGTTCGGA CATTTCAAGA AGTCATGGTT CCAGTATTTG ACATTTTGTT GAGCAGAATA 
AAAGACTTGG ATCTCTGTCA AATCTTGCTA TACACATATC TAGATATGCT TCTGTATTTC 
ACAAGGCAGA AGGATATTGC GAAAGTTTTT GTAGAGTACA TTCAACCCCA GGACCCCAGC 
AATGGACAGA TGTACCAGAA GACTTTACTG GGCGTAATTC TGAGTATCTC CTGCTTGTTA 
AAGACTCCAG GGGTAGTGGA GAATCACAGC TACTTCTTGA ATCCATCCCG ATCCAGTCCC 
CAGGAGATCA AAGTGCAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCACGAAAAG 
ATTTACCAGA TGCTGAAGAA CTTGCTCCAG CTCTCTCCAG ACACAAAGCA TTGGATCTTG 
TCCTGGCTTG GAAATTGCCT ATATGCCAAC ACAGGTCGCA CCAAGATCTG GGCCAATCAG 
ATGCCAGAAA TCTTCTTCCA GATGTATGCC TCAGATGCCT TCTTCCTGAA TCTGGGTGCT 
GCCCTCCTGA AGCTGTGCCA GCCATTTTGC AAACCTAAAT CCCCTAGGCT GCTAACCTTT 
AACCCTACCT ACTGTGCCCT CAAAGAGTTG AATGATGAAG AGAGAAGGAT AAAGAATGTG 
CATATGAAAG GTTTGGATAA AGAGACCTGT TTGATCCCTA AAGAGAGTGA CCAGGAGATG 
CAGTTTGCCC AAAACTATAA CCTGGTAACA GAGAACCTGG TCCTGACACA GTACACCTTA 
CACGTGGGAT TTCACAGGTT GCATGACCAG ATGGTAAAAA TCAACCAGAG CTTGCACCGG 
CTGCAGGTCG CTTGGCGTGA GGCTCAACAA AGTGCCAGCC CCACCGCAGA CAGCCTTCGA 
GAGCAGTTTG AGCGCTTGAT GACGATCTAT CTCTCCACCA AGACTGCCAT GACTGAGCCG 
CAGATGCTAC AAAACTGCCT CAATTTGCAG GTGTCCATGG CCGTTCTTCT GGTTCAGCTA 
GCCAATGGCA ACCAAGGCAC AGAGCCAGTA GAGCTGACCT TCCCTTTGTC AGATCAGTAC 
AGCTCTTTGG CTTATGTGCC AGAATTTTTT GCGGATAACC TGGGAGATTT TCTTATTTTC 
CTACGGCGCT TTGCTGACGA CATCCTGGAG ACATCAGCTG ATTCACTGGA GCAAGTTCTT 
CATTTTGTCA CTATTTTCAC TGGGAGTGTA GAGAGAATGA AAAACCCCCA CCTGAGGGCC 
AAACTGGCAG AGGTGCTGGA AGCAGTCATG CCACACCTGG ACCAAACCCC AAATCCCCTG 
GTGTCCAGCG TGTTCCACCG TAAGCGTGTG TTCTGCGGCT ATCCCTATGC AGCCCAGCTT 
GCTGAGGCAC TCATCAAAGT CTTTGTAGAC ATCGAGTTTA CAGGAGATCC CCATCAGTTT 
GAGCAAAAGT TTAACTACCG CCGCCCCATG TATCCTATCT TAAAGTACAT GTGGGAAACA 
GACACCTACA GAGAAAGCAT AAAGGCTCTA GCTGATTATG CCTCTGCAAA TTTGGAGGCC 
ATGAATCCCC CTCTCTTTCT CCGCTTCCTT AACCTGCTGA TGAATGATGC TATCTTCCTA 
CTGGATGAAG CCATACAGTA CTTGAGCAAG ATAAAGATTC AGCAGATTGA GAGGGACCAA 
GGGGAATGGG ACAATTTGTC TCCAGAGGCC CGCCGAGAAA AGGAGGCCAG TCTGCAAATG 
TTTGGGCAAC TGGCTCGATT CCACAACATC ATGTCAAATG AAACCATTGG TACCTTGGCC 
TTTCTAACAT CAGAGATCAA GTCTCTCTTT GTACATCCCT TCCTGGCTGA ACGAATCATC 
TCTATGTTAA ACTACTTCCT ACAGCACTTG GTTGGTCCTA AGATGGGAGC CTTAAAAGTC 
AAGGACTTCA GCGAGTTTGA CTTCAAACCC CAGCAGCTTG TTTCAGACAT CTGCAACATC 
TACTTAAACC TTGGTGATGA AGAGAATTTC TGTGCCACTG TGCCCAAGGA TGGGCGATCT 
TATTCCCCAA TGCTCTTTGC TCAGACAGTC CGAGTCCTGA AGAAAATAAA CAAGCCTGGA 
AATATGATTG TGGCTTTCAG CAATCTGGCA GAGAAGATCA AGTCTCTTGC AGATCTCCAG 
CAACAGGAGG AAGAGACCTA TGCAGATGCC TGTGATGAGT TTCTAGATCC CATCATGAGT 
ACACTGATGT CAGACCCTGT GATGCTGCCA TCTTCCAGAG TCACTGTGGA TAGGTCCACA 
ATAGCCAGGC ATCTGCTCAG TGATCAGACT GATCCCTTTA ACCGTAGCCC CCTAACTATG 
GACCAGATCC GGCCAAACAC AGAACTGAAA GAGAAAATCC AACGGTGGCT CGCTGAGAGG 
AAATTACAGA AGGAACAACT TGAGTAA