ESS2 cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ESS2
Entrez Gene ID	102457493
Full Name	ess-2 splicing factor homolog
Synonyms	DGCR14
Gene Type	protein-coding
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006134787.2	XP_006134849.2	protein DGCR14
XM_025190542.1	XP_025046327.1	splicing factor ESS-2 homolog

Pathways from BioSystems
Gene Ontology

The following ESS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ESS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe154791	XM_025190542.1 Latest version!	Pelodiscus sinensis ess-2 splicing factor homolog (ESS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OPe39821	XM_006134787.2 Latest version!	Pelodiscus sinensis DiGeorge syndrome critical region gene 14 (DGCR14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ESS2 ( XM_025190542.1 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_025046327.1 Pelodiscus sinensis ess-2 splicing factor homolog (ESS2), mRNA.

Price & Availability↑

CloneID	OPe154791
Clone ID Related Accession (Same CDS sequence)	XM_025190542.1
Accession Version	XM_025190542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1347bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-03
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	splicing factor ESS-2 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005858757.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pelodiscus sinensis Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGTCAACC CTGCTGCTGC AGCAGTGTTG AGAAATCGAG CACTGAGTTT GGAGAAGATT 
ATCCAGAGGG ATTTCTTTCC TGATGTTGAG AGGTTACGTG CTCAGAAGGA GTACTTGGAA 
GCTGAAGAAA GTGGTGACTT AGAGAAAATG AGACAAATAG CAATCAAATT TGGCTCTTCA 
TTGGGGAAAT CATCCAAGGA GACACCTGCC CCATATGTTA CTCCAGCCAC ATTTGAAACA 
CCTGAAGTAC ACCCAGGCGC CTCTTCCTTG TTGAATAAGT CCAAGATTGG GATCAAAACT 
GCAGAAGAAG GAGAAGTGGA GAAAGAACAG AATAAGGATG CTCTTCCCAG CCTTGACAGT 
TTCTTAACCA AACACACCAG CGAAGATAAT GCTTCATTTG AGCAGATCAT GGAAGTGGCC 
AAAGAGAAGG AGAAGGCCAG ACACTCTTGG CTGTACGAAG CAGAAGAGGA GTTCGCTCAG 
CGACGACTTG AGTATCTGGC GCTTCCCTCA GCAGAGCACC AGGCCCTGGA GAGTGGGAAA 
GCTGGAGTGG AGACTTGGGA GTACAAGGCT CAGAATTCCC TGATGTATTA CCCACCAGGT 
GTGCCTGATG AGGATGATGT GTTTAAGAAG CCTCGAGAAG TTGTGCATAG GAACACCCGC 
TTTCTGAAAG ATCCATTCAG TCAAGCACTG AGTAAATCCC AGCTGCAGCA GGCAGCAGCC 
CTCAATGCCC AGTACAAACA GGGCAAAGTA GGGCCAGATG GGAAAGAGCT GATCCCCCAG 
GAGTCTCCAA AAGTGAATGG CTATGGATTT GTGGCCACCC CCTCTCCTGC CCCTGGTGTG 
ACCGATTCTC CTCTTATGAC ATGGGGCGAG ATTGAGAGCA CCCCCCTGCG AATAGATGGG 
TCAGAAACTC CGTATGTGGA CAGGACACCT GGACCAGCTT TCAAGATCCT GGAGCCTGGG 
CGCAGAGAGC GGTTAGGACT CAAGATGGCC AATGAGGCAG CTGCTAAAAA CCGAGCAAAG 
AAACAGGAAG CATTAAGAAG AGTCACGGAA AACTTGGCTA GCCTCACTCC AAAAGGACTG 
AGTCCAGCTA TGTCACCAGC CTTACAACGA CTAGTAAACA GGACTGCGAG CAAATATACT 
GATAAAGCAC TGCGAGCCAG TTATACCCCT TCTCCAGCAC ACCCCGGAAC TCCTGGGTAT 
AAGACCCCAG TAGGTGGGTC ACAGACACCC ACTAGCACCC CACCTCCTAG GACTGTTTCT 
CAGACTCCAG TAACCCAAGA TCCCACTTCA ATCACAGACA ACCTGCTGCA GCTCCCTAAA 
AGACGAAAGG CATCTGATTT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025046327.1
CDS	1..1347
Translation	MVNPAAAAVLRNRALSLEKIIQRDFFPDVERLRAQKEYLEAEESGDLEKMRQIAIKFGSSLGKSSKETPAPYVTPATFETPEVHPGASSLLNKSKIGIKTAEEGEVEKEQNKDALPSLDSFLTKHTSEDNASFEQIMEVAKEKEKARHSWLYEAEEEFAQRRLEYLALPSAEHQALESGKAGVETWEYKAQNSLMYYPPGVPDEDDVFKKPREVVHRNTRFLKDPFSQALSKSQLQQAAALNAQYKQGKVGPDGKELIPQESPKVNGYGFVATPSPAPGVTDSPLMTWGEIESTPLRIDGSETPYVDRTPGPAFKILEPGRRERLGLKMANEAAAKNRAKKQEALRRVTENLASLTPKGLSPAMSPALQRLVNRTASKYTDKALRASYTPSPAHPGTPGYKTPVGGSQTPTSTPPPRTVSQTPVTQDPTSITDNLLQLPKRRKASDFF

Target ORF information:

RefSeq Version	XM_025190542.1
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis ess-2 splicing factor homolog (ESS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025190542.1

ORF Insert Sequence:

ATGGTCAACC CTGCTGCTGC AGCAGTGTTG AGAAATCGAG CACTGAGTTT GGAGAAGATT 
ATCCAGAGGG ATTTCTTTCC TGATGTTGAG AGGTTACGTG CTCAGAAGGA GTACTTGGAA 
GCTGAAGAAA GTGGTGACTT AGAGAAAATG AGACAAATAG CAATCAAATT TGGCTCTTCA 
TTGGGGAAAT CATCCAAGGA GACACCTGCC CCATATGTTA CTCCAGCCAC ATTTGAAACA 
CCTGAAGTAC ACCCAGGCGC CTCTTCCTTG TTGAATAAGT CCAAGATTGG GATCAAAACT 
GCAGAAGAAG GAGAAGTGGA GAAAGAACAG AATAAGGATG CTCTTCCCAG CCTTGACAGT 
TTCTTAACCA AACACACCAG CGAAGATAAT GCTTCATTTG AGCAGATCAT GGAAGTGGCC 
AAAGAGAAGG AGAAGGCCAG ACACTCTTGG CTGTACGAAG CAGAAGAGGA GTTCGCTCAG 
CGACGACTTG AGTATCTGGC GCTTCCCTCA GCAGAGCACC AGGCCCTGGA GAGTGGGAAA 
GCTGGAGTGG AGACTTGGGA GTACAAGGCT CAGAATTCCC TGATGTATTA CCCACCAGGT 
GTGCCTGATG AGGATGATGT GTTTAAGAAG CCTCGAGAAG TTGTGCATAG GAACACCCGC 
TTTCTGAAAG ATCCATTCAG TCAAGCACTG AGTAAATCCC AGCTGCAGCA GGCAGCAGCC 
CTCAATGCCC AGTACAAACA GGGCAAAGTA GGGCCAGATG GGAAAGAGCT GATCCCCCAG 
GAGTCTCCAA AAGTGAATGG CTATGGATTT GTGGCCACCC CCTCTCCTGC CCCTGGTGTG 
ACCGATTCTC CTCTTATGAC ATGGGGCGAG ATTGAGAGCA CCCCCCTGCG AATAGATGGG 
TCAGAAACTC CGTATGTGGA CAGGACACCT GGACCAGCTT TCAAGATCCT GGAGCCTGGG 
CGCAGAGAGC GGTTAGGACT CAAGATGGCC AATGAGGCAG CTGCTAAAAA CCGAGCAAAG 
AAACAGGAAG CATTAAGAAG AGTCACGGAA AACTTGGCTA GCCTCACTCC AAAAGGACTG 
AGTCCAGCTA TGTCACCAGC CTTACAACGA CTAGTAAACA GGACTGCGAG CAAATATACT 
GATAAAGCAC TGCGAGCCAG TTATACCCCT TCTCCAGCAC ACCCCGGAAC TCCTGGGTAT 
AAGACCCCAG TAGGTGGGTC ACAGACACCC ACTAGCACCC CACCTCCTAG GACTGTTTCT 
CAGACTCCAG TAACCCAAGA TCCCACTTCA ATCACAGACA ACCTGCTGCA GCTCCCTAAA 
AGACGAAAGG CATCTGATTT CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ESS2 ( XM_006134787.2 ) cDNA ORF clone, Pelodiscus sinensis(Chinese soft-shelled turtle) -> XP_006134849.2 Pelodiscus sinensis DiGeorge syndrome critical region gene 14 (DGCR14), mRNA.

Price & Availability↑

CloneID	OPe39821
Clone ID Related Accession (Same CDS sequence)	XM_006134787.2
Accession Version	XM_006134787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-02
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Product	protein DGCR14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005858757.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 3, 2015 this sequence version replaced XM_006134787.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pelodiscus sinensis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 33% of CDS bases ##RefSeq-Attributes-END##

ATGTCTTCAG AAGCCCCACT GGACTCCGCG CTCAGCAGAA GAAGTGTGAA GAAGCAATGC 
TCATTCACCA TTGAGAACAT TCTCTCCAGT CCAGTTGAGA GAAGCCCCCA GGTTCTGGTC 
CCGTTCTGTC TCCAGGGGAT CTTGGACTGT GAGCCCAAAG GGCTGTGTGG GCTGGAGGCC 
ATCACTACTG GCTCTCTGCA GGAGGAGGAG GAGGAAGAAG AAGAACTGGA AGCTGCTGGG 
TGCAGCTGCT GTTGCTGTTC TCACACAAGC ACCCATTCCT TGCAGGATAC CTCACATTGG 
CTAGACACCA GATTCCCTTG GCCCATGAGG CTAATTCATC CCACTGCTAG AGCATGTAAG 
AATCTTCATG GGAGCCAGAA TGAGCTGCAG ACTTTCCATC AGCTCCAAAG GCGGACCCGC 
CGGCACCGGA CCATCTTCAC TGAAGATCAG CTACAGGCCC TAGAGGCCCT TTTCAACCAG 
AACCAGTACC CAGATGTCAT CACCAGAGAA CACCTGGCCA ACCGCATCCA CCTGAAGGAG 
GAGCGAGTAG AGCTACAGGC CCTAGAGGCC CTTTTCAACC AGAACCAGTA CCCAGATGTC 
ATCACCAGAG AACACCTGGC CAACCGCATC CACCTGAAGG AGGAGCGAGT AGAGAGTTTG 
GAGAAGATTA TCCAGAGGGA TTTCTTTCCT GATGTTGAGA GGTTACGTGC TCAGAAGGAG 
TACTTGGAAG CTGAAGAAAG TGGTGACTTA GAGAAAATGA GACAAATAGC AATCAAATTT 
GGCTCTTCAT TGGGGAAATC ATCCAAGGAG ACACCTGCCC CATATGTTAC TCCAGCCACA 
TTTGAAACAC CTGAAGTACA CCCAGGCGCC TCTTCCTTGT TGAATAAGTC CAAGATTGGG 
ATCAAAACTG CAGAAGAAGG AGAAGTGGAG AAAGAACAGA ATAAGGATGC TCTTCCCAGC 
CTTGACAGTT TCTTAACCAA ACACACCAGC GAAGATAATG CTTCATTTGA GCAGATCATG 
GAAGTGGCCA AAGAGAAGGA GAAGGCCAGA CACTCTTGGC TGTACGAAGC AGAAGAGGAG 
TTCGCTCAGC GACGACTTGA GTATCTGGCG CTTCCCTCAG CAGAGCACCA GGCCCTGGAG 
AGTGGGAAAG CTGGAGTGGA GACTTGGGAG TACAAGGCTC AGAATTCCCT GATGTATTAC 
CCACCAGGTG TGCCTGATGA GGATGATGTG TTTAAGAAGC CTCGAGAAGT TGTGCATAGG 
AACACCCGCT TTCTGAAAGA TCCATTCAGT CAAGCACTGA GTAAATCCCA GCTGCAGCAG 
GCAGCAGCCC TCAATGCCCA GTACAAACAG GGCAAAGTAG GGCCAGATGG GAAAGAGCTG 
ATCCCCCAGG AGTCTCCAAA AGTGAATGGC TATGGATTTG TGGCCACCCC CTCTCCTGCC 
CCTGGTGTGA CCGATTCTCC TCTTATGACA TGGGGCGAGA TTGAGAGCAC CCCCCTGCGA 
ATAGATGGGT CAGAAACTCC GTATGTGGAC AGGACACCTG GACCAGCTTT CAAGATCCTG 
GAGCCTGGGC GCAGAGAGCG GTTAGGACTC AAGATGGCCA ATGAGGCAGC TGCTAAAAAC 
CGAGCAAAGA AACAGGAAGC ATTAAGAAGA GTCACGGAAA ACTTGGCTAG CCTCACTCCA 
AAAGGACTGA GTCCAGCTAT GTCACCAGCC TTACAACGAC TAGTAAACAG GACTGCGAGC 
AAATATACTG ATAAAGCACT GCGAGCCAGT TATACCCCTT CTCCAGCACA CCCCGGAACT 
CCTGGGTATA AGACCCCAGT AGGTGGGTCA CAGACACCCA CTAGCACCCC ACCTCCTAGG 
ACTGTTTCTC AGACTCCAGT AACCCAAGAT CCCACTTCAA TCACAGACAA CCTGCTGCAG 
CTCCCTAAAA GACGAAAGGC ATCTGATTTC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006134849.2
CDS	1..1956
Translation	MSSEAPLDSALSRRSVKKQCSFTIENILSSPVERSPQVLVPFCLQGILDCEPKGLCGLEAITTGSLQEEEEEEEELEAAGCSCCCCSHTSTHSLQDTSHWLDTRFPWPMRLIHPTARACKNLHGSQNELQTFHQLQRRTRRHRTIFTEDQLQALEALFNQNQYPDVITREHLANRIHLKEERVELQALEALFNQNQYPDVITREHLANRIHLKEERVESLEKIIQRDFFPDVERLRAQKEYLEAEESGDLEKMRQIAIKFGSSLGKSSKETPAPYVTPATFETPEVHPGASSLLNKSKIGIKTAEEGEVEKEQNKDALPSLDSFLTKHTSEDNASFEQIMEVAKEKEKARHSWLYEAEEEFAQRRLEYLALPSAEHQALESGKAGVETWEYKAQNSLMYYPPGVPDEDDVFKKPREVVHRNTRFLKDPFSQALSKSQLQQAAALNAQYKQGKVGPDGKELIPQESPKVNGYGFVATPSPAPGVTDSPLMTWGEIESTPLRIDGSETPYVDRTPGPAFKILEPGRRERLGLKMANEAAAKNRAKKQEALRRVTENLASLTPKGLSPAMSPALQRLVNRTASKYTDKALRASYTPSPAHPGTPGYKTPVGGSQTPTSTPPPRTVSQTPVTQDPTSITDNLLQLPKRRKASDFF

Target ORF information:

RefSeq Version	XM_006134787.2
Organism	Pelodiscus sinensis(Chinese soft-shelled turtle)
Definition	Pelodiscus sinensis DiGeorge syndrome critical region gene 14 (DGCR14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006134787.2

ORF Insert Sequence:

ATGTCTTCAG AAGCCCCACT GGACTCCGCG CTCAGCAGAA GAAGTGTGAA GAAGCAATGC 
TCATTCACCA TTGAGAACAT TCTCTCCAGT CCAGTTGAGA GAAGCCCCCA GGTTCTGGTC 
CCGTTCTGTC TCCAGGGGAT CTTGGACTGT GAGCCCAAAG GGCTGTGTGG GCTGGAGGCC 
ATCACTACTG GCTCTCTGCA GGAGGAGGAG GAGGAAGAAG AAGAACTGGA AGCTGCTGGG 
TGCAGCTGCT GTTGCTGTTC TCACACAAGC ACCCATTCCT TGCAGGATAC CTCACATTGG 
CTAGACACCA GATTCCCTTG GCCCATGAGG CTAATTCATC CCACTGCTAG AGCATGTAAG 
AATCTTCATG GGAGCCAGAA TGAGCTGCAG ACTTTCCATC AGCTCCAAAG GCGGACCCGC 
CGGCACCGGA CCATCTTCAC TGAAGATCAG CTACAGGCCC TAGAGGCCCT TTTCAACCAG 
AACCAGTACC CAGATGTCAT CACCAGAGAA CACCTGGCCA ACCGCATCCA CCTGAAGGAG 
GAGCGAGTAG AGCTACAGGC CCTAGAGGCC CTTTTCAACC AGAACCAGTA CCCAGATGTC 
ATCACCAGAG AACACCTGGC CAACCGCATC CACCTGAAGG AGGAGCGAGT AGAGAGTTTG 
GAGAAGATTA TCCAGAGGGA TTTCTTTCCT GATGTTGAGA GGTTACGTGC TCAGAAGGAG 
TACTTGGAAG CTGAAGAAAG TGGTGACTTA GAGAAAATGA GACAAATAGC AATCAAATTT 
GGCTCTTCAT TGGGGAAATC ATCCAAGGAG ACACCTGCCC CATATGTTAC TCCAGCCACA 
TTTGAAACAC CTGAAGTACA CCCAGGCGCC TCTTCCTTGT TGAATAAGTC CAAGATTGGG 
ATCAAAACTG CAGAAGAAGG AGAAGTGGAG AAAGAACAGA ATAAGGATGC TCTTCCCAGC 
CTTGACAGTT TCTTAACCAA ACACACCAGC GAAGATAATG CTTCATTTGA GCAGATCATG 
GAAGTGGCCA AAGAGAAGGA GAAGGCCAGA CACTCTTGGC TGTACGAAGC AGAAGAGGAG 
TTCGCTCAGC GACGACTTGA GTATCTGGCG CTTCCCTCAG CAGAGCACCA GGCCCTGGAG 
AGTGGGAAAG CTGGAGTGGA GACTTGGGAG TACAAGGCTC AGAATTCCCT GATGTATTAC 
CCACCAGGTG TGCCTGATGA GGATGATGTG TTTAAGAAGC CTCGAGAAGT TGTGCATAGG 
AACACCCGCT TTCTGAAAGA TCCATTCAGT CAAGCACTGA GTAAATCCCA GCTGCAGCAG 
GCAGCAGCCC TCAATGCCCA GTACAAACAG GGCAAAGTAG GGCCAGATGG GAAAGAGCTG 
ATCCCCCAGG AGTCTCCAAA AGTGAATGGC TATGGATTTG TGGCCACCCC CTCTCCTGCC 
CCTGGTGTGA CCGATTCTCC TCTTATGACA TGGGGCGAGA TTGAGAGCAC CCCCCTGCGA 
ATAGATGGGT CAGAAACTCC GTATGTGGAC AGGACACCTG GACCAGCTTT CAAGATCCTG 
GAGCCTGGGC GCAGAGAGCG GTTAGGACTC AAGATGGCCA ATGAGGCAGC TGCTAAAAAC 
CGAGCAAAGA AACAGGAAGC ATTAAGAAGA GTCACGGAAA ACTTGGCTAG CCTCACTCCA 
AAAGGACTGA GTCCAGCTAT GTCACCAGCC TTACAACGAC TAGTAAACAG GACTGCGAGC 
AAATATACTG ATAAAGCACT GCGAGCCAGT TATACCCCTT CTCCAGCACA CCCCGGAACT 
CCTGGGTATA AGACCCCAGT AGGTGGGTCA CAGACACCCA CTAGCACCCC ACCTCCTAGG 
ACTGTTTCTC AGACTCCAGT AACCCAAGAT CCCACTTCAA TCACAGACAA CCTGCTGCAG 
CTCCCTAAAA GACGAAAGGC ATCTGATTTC TTCTAA