ECE2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECE2
Entrez Gene ID	104001071
Full Name	endothelin converting enzyme 2
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001148031.2	XP_001148031.2	endothelin-converting enzyme 2 isoform X3
XM_009446942.3	XP_009445217.2	endothelin-converting enzyme 2 isoform X1
XM_009446942.2	XP_009445217.2	endothelin-converting enzyme 2 isoform X1
XM_001148106.3	XP_001148106.2	endothelin-converting enzyme 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following ECE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc83041	XM_009446942.3 Latest version!	Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPc83042	XM_001148106.3 Latest version!	Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OPc83043	XM_001148031.2 Latest version!	Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OPc83041	XM_009446942.2	Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECE2 ( XM_009446942.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445217.2 Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc83041
Clone ID Related Accession (Same CDS sequence)	XM_009446942.3 , XM_009446942.2
Accession Version	XM_009446942.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	endothelin-converting enzyme 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_009446942.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTCG CGCTGCAGGA GCTGGGAGCT GGCAGCAACA TGGTGGAGTA CAAACGGGCC 
ACGCTTCGGG ATGAAGACGC ACCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC 
ATGGAGGTGG GCAAGGGGGC TTCCCCTTTC TCACCAGGCC CCAGCCCTGG CATGACGCCT 
GGCACACCCA GGAGCTCTGG GCTGTTCTGG AGGGTCACCT GCCCCCACCT CCGCTCCATC 
TCTGGCCTCT GCTCTAGGAC TATGGTGGGA TTCCAGAAGG GGACAAGACA GCTGTTAGGC 
TCACGCACGC AGCTGGAGCT GGTCTTAGCA GGTGTCTCTC TACTGCTGGC TGCACTGCTT 
CTGGGCTGCC TTGTGGCCCT AGGGGTCCAG TACCACAGAG ACCCATCCCA CAGCACCTGC 
CTTACAGAGG CCTGCATTCG AGTGGCTGGA AAAATCCTGG AGTCCCTGGA CCGAGGGGTG 
AGCCCCTGTG AGGACTTTTA CCAGTTCTCC TGTGGGGGCT GGATTCGGAG GAACCCCCTG 
CCCGATGGGC GTTCTCGCTG GAACACCTTC AACAGCCTCT GGGACCAAAA CCAGGCCATA 
CTGAAGCACC TGCTTGAAAA CACCACCTTC AACTCCAGCA GTGAAGCTGA GCGGAAGACA 
CAGCGCTTCT ACCTATCTTG CCTACAGGTG GAGCGCATTG AGGAGCTGGG AGCCCAGCCA 
CTGAGAGACC TCATTGACAA GATTGGTGGT TGGAACATTA CGGGGCCCTG GGACCAGGAC 
AACTTTATGG AGGTGTTGAA GGCAGTAGCA GGGACCTACA GGGCCACCCC ATTCTTCACC 
GTCTACATCA GTGCCGACTC TAAGAGTTCC AACAGCAATG TTATCCAGGT GGACCAGTCT 
GGGCTCTTTC TGCCCTCTCG GGATTACTAC TTAAACAGAA CTGCCAATGA GAAAGTGCTC 
ACTGCCTATC TGGATTACAT GGAGGAACTG GGGCTGCTGC TGGGTGGGCG GCCCACCTCC 
ACGAGGGAGC AGATGCAGCA GGTGCTGGAG TTGGAGATAC AGCTGGCCAA CATCACAGTG 
CCCCAGGACC AGCGGCGCGA CGAGGAGAAG ATCTACCACA AGATGAGCAT TTCGGAGCTG 
CAGGCTCTGG CGCCCTCCAT GGACTGGCTT GAGTTCCTGT CTTTCTTGCT GTCACCATTG 
GAGTTGAGTG ACTCTGAGCC TGTGGTGGTG TATGGGATGG ATTATTTGCA GCAGGTGTCA 
GAGCTCATCA ACCGCACGGA ACCAAGCATC CTGAACAATT ACCTGATCTG GAACCTGGTG 
CAAAAGACAA CCTCAAGCCT GGACCGACGC TTTGAGTCTG CACAAGAGAA GCTGCTGGAG 
ACCCTCTATG GCACTAAGAA GTCCTGTGTG CCGAGGTGGC AGACCTGCAT CTCCAACACG 
GATGACGCCC TTGGCTTCGC TTTGGGGTCC CTCTTCGTGA AGGCCACGTT TGACCGGCAA 
AGCAAAGAAA TTGCAGAGGG GATGATCAGC GAAATCCGGA CCGCATTTGA GGAGGCCCTG 
GGACAGCTGG TTTGGATGGA TGAGAAGACC CGCCAGGCAG CCAAGGAGAA AGCAGATGCC 
ATCTATGATA TGATTGGTTT CCCAGACTTT ATCCTGGAGC CCAAAGAGCT GGATGATGTT 
TATGACGGGT ACGAAATTTC TGAAGATTCT TTCTTCCAAA ACATGTTGAA TTTGTACAAC 
TTCTCTGCCA AGGTTATGGC TGACCAGCTC CGCAAGCCTC CCAGCCGAGA CCAGTGGAGC 
ATGACCCCCC AGACAGTGAA TGCCTACTAC CTTCCAACTA AGAATGAGAT CGTCTTCCCC 
GCTGGCATCC TGCAGGCCCC CTTCTATGCC CGCAACCACC CCAAGGCCCT GAACTTTGGT 
GGCATCGGTG TGGTCATGGG CCATGAGTTG ACGCATGCCT TTGATGACCA AGGGCGCGAG 
TATGACAAAG AAGGGAACCT GCGGCCCTGG TGGCAGAATG AGTCCCTGGC AGCCTTCCGG 
AACCACACGG CCTGCATGGA GGAACAGTAC AATCAATACC AGGTCAATGG GGAGAGGCTC 
AACGGCCGCC AGACGCTGGG GGAGAACATT GCTGACAACG GGGGGCTGAA GGCTGCCTAC 
AATGCTTACA AAGCATGGCT GAGAAAGCAT GGGGAGGAGC AGCAACTGCC AGCCGTGGGG 
CTCACCAACC ACCAGCTCTT CTTCGTGGGA TTTGCCCAGG TGTGGTGCTC GGTCCGCACA 
CCAGAGAGCT CTCACGAGGG GCTGGTGACC GACCCCCACA GCCCTGCCCG CTTCCGCGTG 
CTGGGCACTC TCTCCAACTC CCGTGACTTC CTGCGGCACT TCGGCTGCCC TGTCGGCTCC 
CCCATGAACC CAGGGCAGCT GTGTGAGGTG TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445217.2
CDS	137..2572
Translation	MNVALQELGAGSNMVEYKRATLRDEDAPETPVEGGASPDAMEVGKGASPFSPGPSPGMTPGTPRSSGLFWRVTCPHLRSISGLCSRTMVGFQKGTRQLLGSRTQLELVLAGVSLLLAALLLGCLVALGVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTQRFYLSCLQVERIEELGAQPLRDLIDKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGLLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW

Target ORF information:

RefSeq Version	XM_009446942.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009446942.3

ORF Insert Sequence:

ATGAACGTCG CGCTGCAGGA GCTGGGAGCT GGCAGCAACA TGGTGGAGTA CAAACGGGCC 
ACGCTTCGGG ATGAAGACGC ACCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC 
ATGGAGGTGG GCAAGGGGGC TTCCCCTTTC TCACCAGGCC CCAGCCCTGG CATGACGCCT 
GGCACACCCA GGAGCTCTGG GCTGTTCTGG AGGGTCACCT GCCCCCACCT CCGCTCCATC 
TCTGGCCTCT GCTCTAGGAC TATGGTGGGA TTCCAGAAGG GGACAAGACA GCTGTTAGGC 
TCACGCACGC AGCTGGAGCT GGTCTTAGCA GGTGTCTCTC TACTGCTGGC TGCACTGCTT 
CTGGGCTGCC TTGTGGCCCT AGGGGTCCAG TACCACAGAG ACCCATCCCA CAGCACCTGC 
CTTACAGAGG CCTGCATTCG AGTGGCTGGA AAAATCCTGG AGTCCCTGGA CCGAGGGGTG 
AGCCCCTGTG AGGACTTTTA CCAGTTCTCC TGTGGGGGCT GGATTCGGAG GAACCCCCTG 
CCCGATGGGC GTTCTCGCTG GAACACCTTC AACAGCCTCT GGGACCAAAA CCAGGCCATA 
CTGAAGCACC TGCTTGAAAA CACCACCTTC AACTCCAGCA GTGAAGCTGA GCGGAAGACA 
CAGCGCTTCT ACCTATCTTG CCTACAGGTG GAGCGCATTG AGGAGCTGGG AGCCCAGCCA 
CTGAGAGACC TCATTGACAA GATTGGTGGT TGGAACATTA CGGGGCCCTG GGACCAGGAC 
AACTTTATGG AGGTGTTGAA GGCAGTAGCA GGGACCTACA GGGCCACCCC ATTCTTCACC 
GTCTACATCA GTGCCGACTC TAAGAGTTCC AACAGCAATG TTATCCAGGT GGACCAGTCT 
GGGCTCTTTC TGCCCTCTCG GGATTACTAC TTAAACAGAA CTGCCAATGA GAAAGTGCTC 
ACTGCCTATC TGGATTACAT GGAGGAACTG GGGCTGCTGC TGGGTGGGCG GCCCACCTCC 
ACGAGGGAGC AGATGCAGCA GGTGCTGGAG TTGGAGATAC AGCTGGCCAA CATCACAGTG 
CCCCAGGACC AGCGGCGCGA CGAGGAGAAG ATCTACCACA AGATGAGCAT TTCGGAGCTG 
CAGGCTCTGG CGCCCTCCAT GGACTGGCTT GAGTTCCTGT CTTTCTTGCT GTCACCATTG 
GAGTTGAGTG ACTCTGAGCC TGTGGTGGTG TATGGGATGG ATTATTTGCA GCAGGTGTCA 
GAGCTCATCA ACCGCACGGA ACCAAGCATC CTGAACAATT ACCTGATCTG GAACCTGGTG 
CAAAAGACAA CCTCAAGCCT GGACCGACGC TTTGAGTCTG CACAAGAGAA GCTGCTGGAG 
ACCCTCTATG GCACTAAGAA GTCCTGTGTG CCGAGGTGGC AGACCTGCAT CTCCAACACG 
GATGACGCCC TTGGCTTCGC TTTGGGGTCC CTCTTCGTGA AGGCCACGTT TGACCGGCAA 
AGCAAAGAAA TTGCAGAGGG GATGATCAGC GAAATCCGGA CCGCATTTGA GGAGGCCCTG 
GGACAGCTGG TTTGGATGGA TGAGAAGACC CGCCAGGCAG CCAAGGAGAA AGCAGATGCC 
ATCTATGATA TGATTGGTTT CCCAGACTTT ATCCTGGAGC CCAAAGAGCT GGATGATGTT 
TATGACGGGT ACGAAATTTC TGAAGATTCT TTCTTCCAAA ACATGTTGAA TTTGTACAAC 
TTCTCTGCCA AGGTTATGGC TGACCAGCTC CGCAAGCCTC CCAGCCGAGA CCAGTGGAGC 
ATGACCCCCC AGACAGTGAA TGCCTACTAC CTTCCAACTA AGAATGAGAT CGTCTTCCCC 
GCTGGCATCC TGCAGGCCCC CTTCTATGCC CGCAACCACC CCAAGGCCCT GAACTTTGGT 
GGCATCGGTG TGGTCATGGG CCATGAGTTG ACGCATGCCT TTGATGACCA AGGGCGCGAG 
TATGACAAAG AAGGGAACCT GCGGCCCTGG TGGCAGAATG AGTCCCTGGC AGCCTTCCGG 
AACCACACGG CCTGCATGGA GGAACAGTAC AATCAATACC AGGTCAATGG GGAGAGGCTC 
AACGGCCGCC AGACGCTGGG GGAGAACATT GCTGACAACG GGGGGCTGAA GGCTGCCTAC 
AATGCTTACA AAGCATGGCT GAGAAAGCAT GGGGAGGAGC AGCAACTGCC AGCCGTGGGG 
CTCACCAACC ACCAGCTCTT CTTCGTGGGA TTTGCCCAGG TGTGGTGCTC GGTCCGCACA 
CCAGAGAGCT CTCACGAGGG GCTGGTGACC GACCCCCACA GCCCTGCCCG CTTCCGCGTG 
CTGGGCACTC TCTCCAACTC CCGTGACTTC CTGCGGCACT TCGGCTGCCC TGTCGGCTCC 
CCCATGAACC CAGGGCAGCT GTGTGAGGTG TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE2 ( XM_009446942.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_009445217.2 Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc83041
Clone ID Related Accession (Same CDS sequence)	XM_009446942.3 , XM_009446942.2
Accession Version	XM_009446942.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	endothelin-converting enzyme 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006490.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_009446942.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTCG CGCTGCAGGA GCTGGGAGCT GGCAGCAACA TGGTGGAGTA CAAACGGGCC 
ACGCTTCGGG ATGAAGACGC ACCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC 
ATGGAGGTGG GCAAGGGGGC TTCCCCTTTC TCACCAGGCC CCAGCCCTGG CATGACGCCT 
GGCACACCCA GGAGCTCTGG GCTGTTCTGG AGGGTCACCT GCCCCCACCT CCGCTCCATC 
TCTGGCCTCT GCTCTAGGAC TATGGTGGGA TTCCAGAAGG GGACAAGACA GCTGTTAGGC 
TCACGCACGC AGCTGGAGCT GGTCTTAGCA GGTGTCTCTC TACTGCTGGC TGCACTGCTT 
CTGGGCTGCC TTGTGGCCCT AGGGGTCCAG TACCACAGAG ACCCATCCCA CAGCACCTGC 
CTTACAGAGG CCTGCATTCG AGTGGCTGGA AAAATCCTGG AGTCCCTGGA CCGAGGGGTG 
AGCCCCTGTG AGGACTTTTA CCAGTTCTCC TGTGGGGGCT GGATTCGGAG GAACCCCCTG 
CCCGATGGGC GTTCTCGCTG GAACACCTTC AACAGCCTCT GGGACCAAAA CCAGGCCATA 
CTGAAGCACC TGCTTGAAAA CACCACCTTC AACTCCAGCA GTGAAGCTGA GCGGAAGACA 
CAGCGCTTCT ACCTATCTTG CCTACAGGTG GAGCGCATTG AGGAGCTGGG AGCCCAGCCA 
CTGAGAGACC TCATTGACAA GATTGGTGGT TGGAACATTA CGGGGCCCTG GGACCAGGAC 
AACTTTATGG AGGTGTTGAA GGCAGTAGCA GGGACCTACA GGGCCACCCC ATTCTTCACC 
GTCTACATCA GTGCCGACTC TAAGAGTTCC AACAGCAATG TTATCCAGGT GGACCAGTCT 
GGGCTCTTTC TGCCCTCTCG GGATTACTAC TTAAACAGAA CTGCCAATGA GAAAGTGCTC 
ACTGCCTATC TGGATTACAT GGAGGAACTG GGGCTGCTGC TGGGTGGGCG GCCCACCTCC 
ACGAGGGAGC AGATGCAGCA GGTGCTGGAG TTGGAGATAC AGCTGGCCAA CATCACAGTG 
CCCCAGGACC AGCGGCGCGA CGAGGAGAAG ATCTACCACA AGATGAGCAT TTCGGAGCTG 
CAGGCTCTGG CGCCCTCCAT GGACTGGCTT GAGTTCCTGT CTTTCTTGCT GTCACCATTG 
GAGTTGAGTG ACTCTGAGCC TGTGGTGGTG TATGGGATGG ATTATTTGCA GCAGGTGTCA 
GAGCTCATCA ACCGCACGGA ACCAAGCATC CTGAACAATT ACCTGATCTG GAACCTGGTG 
CAAAAGACAA CCTCAAGCCT GGACCGACGC TTTGAGTCTG CACAAGAGAA GCTGCTGGAG 
ACCCTCTATG GCACTAAGAA GTCCTGTGTG CCGAGGTGGC AGACCTGCAT CTCCAACACG 
GATGACGCCC TTGGCTTCGC TTTGGGGTCC CTCTTCGTGA AGGCCACGTT TGACCGGCAA 
AGCAAAGAAA TTGCAGAGGG GATGATCAGC GAAATCCGGA CCGCATTTGA GGAGGCCCTG 
GGACAGCTGG TTTGGATGGA TGAGAAGACC CGCCAGGCAG CCAAGGAGAA AGCAGATGCC 
ATCTATGATA TGATTGGTTT CCCAGACTTT ATCCTGGAGC CCAAAGAGCT GGATGATGTT 
TATGACGGGT ACGAAATTTC TGAAGATTCT TTCTTCCAAA ACATGTTGAA TTTGTACAAC 
TTCTCTGCCA AGGTTATGGC TGACCAGCTC CGCAAGCCTC CCAGCCGAGA CCAGTGGAGC 
ATGACCCCCC AGACAGTGAA TGCCTACTAC CTTCCAACTA AGAATGAGAT CGTCTTCCCC 
GCTGGCATCC TGCAGGCCCC CTTCTATGCC CGCAACCACC CCAAGGCCCT GAACTTTGGT 
GGCATCGGTG TGGTCATGGG CCATGAGTTG ACGCATGCCT TTGATGACCA AGGGCGCGAG 
TATGACAAAG AAGGGAACCT GCGGCCCTGG TGGCAGAATG AGTCCCTGGC AGCCTTCCGG 
AACCACACGG CCTGCATGGA GGAACAGTAC AATCAATACC AGGTCAATGG GGAGAGGCTC 
AACGGCCGCC AGACGCTGGG GGAGAACATT GCTGACAACG GGGGGCTGAA GGCTGCCTAC 
AATGCTTACA AAGCATGGCT GAGAAAGCAT GGGGAGGAGC AGCAACTGCC AGCCGTGGGG 
CTCACCAACC ACCAGCTCTT CTTCGTGGGA TTTGCCCAGG TGTGGTGCTC GGTCCGCACA 
CCAGAGAGCT CTCACGAGGG GCTGGTGACC GACCCCCACA GCCCTGCCCG CTTCCGCGTG 
CTGGGCACTC TCTCCAACTC CCGTGACTTC CTGCGGCACT TCGGCTGCCC TGTCGGCTCC 
CCCATGAACC CAGGGCAGCT GTGTGAGGTG TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009445217.2
CDS	136..2571
Translation	MNVALQELGAGSNMVEYKRATLRDEDAPETPVEGGASPDAMEVGKGASPFSPGPSPGMTPGTPRSSGLFWRVTCPHLRSISGLCSRTMVGFQKGTRQLLGSRTQLELVLAGVSLLLAALLLGCLVALGVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTQRFYLSCLQVERIEELGAQPLRDLIDKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGLLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW

Target ORF information:

RefSeq Version	XM_009446942.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009446942.2

ORF Insert Sequence:

ATGAACGTCG CGCTGCAGGA GCTGGGAGCT GGCAGCAACA TGGTGGAGTA CAAACGGGCC 
ACGCTTCGGG ATGAAGACGC ACCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC 
ATGGAGGTGG GCAAGGGGGC TTCCCCTTTC TCACCAGGCC CCAGCCCTGG CATGACGCCT 
GGCACACCCA GGAGCTCTGG GCTGTTCTGG AGGGTCACCT GCCCCCACCT CCGCTCCATC 
TCTGGCCTCT GCTCTAGGAC TATGGTGGGA TTCCAGAAGG GGACAAGACA GCTGTTAGGC 
TCACGCACGC AGCTGGAGCT GGTCTTAGCA GGTGTCTCTC TACTGCTGGC TGCACTGCTT 
CTGGGCTGCC TTGTGGCCCT AGGGGTCCAG TACCACAGAG ACCCATCCCA CAGCACCTGC 
CTTACAGAGG CCTGCATTCG AGTGGCTGGA AAAATCCTGG AGTCCCTGGA CCGAGGGGTG 
AGCCCCTGTG AGGACTTTTA CCAGTTCTCC TGTGGGGGCT GGATTCGGAG GAACCCCCTG 
CCCGATGGGC GTTCTCGCTG GAACACCTTC AACAGCCTCT GGGACCAAAA CCAGGCCATA 
CTGAAGCACC TGCTTGAAAA CACCACCTTC AACTCCAGCA GTGAAGCTGA GCGGAAGACA 
CAGCGCTTCT ACCTATCTTG CCTACAGGTG GAGCGCATTG AGGAGCTGGG AGCCCAGCCA 
CTGAGAGACC TCATTGACAA GATTGGTGGT TGGAACATTA CGGGGCCCTG GGACCAGGAC 
AACTTTATGG AGGTGTTGAA GGCAGTAGCA GGGACCTACA GGGCCACCCC ATTCTTCACC 
GTCTACATCA GTGCCGACTC TAAGAGTTCC AACAGCAATG TTATCCAGGT GGACCAGTCT 
GGGCTCTTTC TGCCCTCTCG GGATTACTAC TTAAACAGAA CTGCCAATGA GAAAGTGCTC 
ACTGCCTATC TGGATTACAT GGAGGAACTG GGGCTGCTGC TGGGTGGGCG GCCCACCTCC 
ACGAGGGAGC AGATGCAGCA GGTGCTGGAG TTGGAGATAC AGCTGGCCAA CATCACAGTG 
CCCCAGGACC AGCGGCGCGA CGAGGAGAAG ATCTACCACA AGATGAGCAT TTCGGAGCTG 
CAGGCTCTGG CGCCCTCCAT GGACTGGCTT GAGTTCCTGT CTTTCTTGCT GTCACCATTG 
GAGTTGAGTG ACTCTGAGCC TGTGGTGGTG TATGGGATGG ATTATTTGCA GCAGGTGTCA 
GAGCTCATCA ACCGCACGGA ACCAAGCATC CTGAACAATT ACCTGATCTG GAACCTGGTG 
CAAAAGACAA CCTCAAGCCT GGACCGACGC TTTGAGTCTG CACAAGAGAA GCTGCTGGAG 
ACCCTCTATG GCACTAAGAA GTCCTGTGTG CCGAGGTGGC AGACCTGCAT CTCCAACACG 
GATGACGCCC TTGGCTTCGC TTTGGGGTCC CTCTTCGTGA AGGCCACGTT TGACCGGCAA 
AGCAAAGAAA TTGCAGAGGG GATGATCAGC GAAATCCGGA CCGCATTTGA GGAGGCCCTG 
GGACAGCTGG TTTGGATGGA TGAGAAGACC CGCCAGGCAG CCAAGGAGAA AGCAGATGCC 
ATCTATGATA TGATTGGTTT CCCAGACTTT ATCCTGGAGC CCAAAGAGCT GGATGATGTT 
TATGACGGGT ACGAAATTTC TGAAGATTCT TTCTTCCAAA ACATGTTGAA TTTGTACAAC 
TTCTCTGCCA AGGTTATGGC TGACCAGCTC CGCAAGCCTC CCAGCCGAGA CCAGTGGAGC 
ATGACCCCCC AGACAGTGAA TGCCTACTAC CTTCCAACTA AGAATGAGAT CGTCTTCCCC 
GCTGGCATCC TGCAGGCCCC CTTCTATGCC CGCAACCACC CCAAGGCCCT GAACTTTGGT 
GGCATCGGTG TGGTCATGGG CCATGAGTTG ACGCATGCCT TTGATGACCA AGGGCGCGAG 
TATGACAAAG AAGGGAACCT GCGGCCCTGG TGGCAGAATG AGTCCCTGGC AGCCTTCCGG 
AACCACACGG CCTGCATGGA GGAACAGTAC AATCAATACC AGGTCAATGG GGAGAGGCTC 
AACGGCCGCC AGACGCTGGG GGAGAACATT GCTGACAACG GGGGGCTGAA GGCTGCCTAC 
AATGCTTACA AAGCATGGCT GAGAAAGCAT GGGGAGGAGC AGCAACTGCC AGCCGTGGGG 
CTCACCAACC ACCAGCTCTT CTTCGTGGGA TTTGCCCAGG TGTGGTGCTC GGTCCGCACA 
CCAGAGAGCT CTCACGAGGG GCTGGTGACC GACCCCCACA GCCCTGCCCG CTTCCGCGTG 
CTGGGCACTC TCTCCAACTC CCGTGACTTC CTGCGGCACT TCGGCTGCCC TGTCGGCTCC 
CCCATGAACC CAGGGCAGCT GTGTGAGGTG TGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE2 ( XM_001148106.3 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001148106.2 Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X2, mRNA.

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CloneID	OPc83042
Clone ID Related Accession (Same CDS sequence)	XM_001148106.3
Accession Version	XM_001148106.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	endothelin-converting enzyme 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001148106.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTCG CGCTGCAGGA GCTGGGAGCT GGCAGCAACA TGGTGGAGTA CAAACGGGCC 
ACGCTTCGGG ATGAAGACGC ACCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC 
ATGGAGGTGG GATTCCAGAA GGGGACAAGA CAGCTGTTAG GCTCACGCAC GCAGCTGGAG 
CTGGTCTTAG CAGGTGTCTC TCTACTGCTG GCTGCACTGC TTCTGGGCTG CCTTGTGGCC 
CTAGGGGTCC AGTACCACAG AGACCCATCC CACAGCACCT GCCTTACAGA GGCCTGCATT 
CGAGTGGCTG GAAAAATCCT GGAGTCCCTG GACCGAGGGG TGAGCCCCTG TGAGGACTTT 
TACCAGTTCT CCTGTGGGGG CTGGATTCGG AGGAACCCCC TGCCCGATGG GCGTTCTCGC 
TGGAACACCT TCAACAGCCT CTGGGACCAA AACCAGGCCA TACTGAAGCA CCTGCTTGAA 
AACACCACCT TCAACTCCAG CAGTGAAGCT GAGCGGAAGA CACAGCGCTT CTACCTATCT 
TGCCTACAGG TGGAGCGCAT TGAGGAGCTG GGAGCCCAGC CACTGAGAGA CCTCATTGAC 
AAGATTGGTG GTTGGAACAT TACGGGGCCC TGGGACCAGG ACAACTTTAT GGAGGTGTTG 
AAGGCAGTAG CAGGGACCTA CAGGGCCACC CCATTCTTCA CCGTCTACAT CAGTGCCGAC 
TCTAAGAGTT CCAACAGCAA TGTTATCCAG GTGGACCAGT CTGGGCTCTT TCTGCCCTCT 
CGGGATTACT ACTTAAACAG AACTGCCAAT GAGAAAGTGC TCACTGCCTA TCTGGATTAC 
ATGGAGGAAC TGGGGCTGCT GCTGGGTGGG CGGCCCACCT CCACGAGGGA GCAGATGCAG 
CAGGTGCTGG AGTTGGAGAT ACAGCTGGCC AACATCACAG TGCCCCAGGA CCAGCGGCGC 
GACGAGGAGA AGATCTACCA CAAGATGAGC ATTTCGGAGC TGCAGGCTCT GGCGCCCTCC 
ATGGACTGGC TTGAGTTCCT GTCTTTCTTG CTGTCACCAT TGGAGTTGAG TGACTCTGAG 
CCTGTGGTGG TGTATGGGAT GGATTATTTG CAGCAGGTGT CAGAGCTCAT CAACCGCACG 
GAACCAAGCA TCCTGAACAA TTACCTGATC TGGAACCTGG TGCAAAAGAC AACCTCAAGC 
CTGGACCGAC GCTTTGAGTC TGCACAAGAG AAGCTGCTGG AGACCCTCTA TGGCACTAAG 
AAGTCCTGTG TGCCGAGGTG GCAGACCTGC ATCTCCAACA CGGATGACGC CCTTGGCTTC 
GCTTTGGGGT CCCTCTTCGT GAAGGCCACG TTTGACCGGC AAAGCAAAGA AATTGCAGAG 
GGGATGATCA GCGAAATCCG GACCGCATTT GAGGAGGCCC TGGGACAGCT GGTTTGGATG 
GATGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CCATCTATGA TATGATTGGT 
TTCCCAGACT TTATCCTGGA GCCCAAAGAG CTGGATGATG TTTATGACGG GTACGAAATT 
TCTGAAGATT CTTTCTTCCA AAACATGTTG AATTTGTACA ACTTCTCTGC CAAGGTTATG 
GCTGACCAGC TCCGCAAGCC TCCCAGCCGA GACCAGTGGA GCATGACCCC CCAGACAGTG 
AATGCCTACT ACCTTCCAAC TAAGAATGAG ATCGTCTTCC CCGCTGGCAT CCTGCAGGCC 
CCCTTCTATG CCCGCAACCA CCCCAAGGCC CTGAACTTTG GTGGCATCGG TGTGGTCATG 
GGCCATGAGT TGACGCATGC CTTTGATGAC CAAGGGCGCG AGTATGACAA AGAAGGGAAC 
CTGCGGCCCT GGTGGCAGAA TGAGTCCCTG GCAGCCTTCC GGAACCACAC GGCCTGCATG 
GAGGAACAGT ACAATCAATA CCAGGTCAAT GGGGAGAGGC TCAACGGCCG CCAGACGCTG 
GGGGAGAACA TTGCTGACAA CGGGGGGCTG AAGGCTGCCT ACAATGCTTA CAAAGCATGG 
CTGAGAAAGC ATGGGGAGGA GCAGCAACTG CCAGCCGTGG GGCTCACCAA CCACCAGCTC 
TTCTTCGTGG GATTTGCCCA GGTGTGGTGC TCGGTCCGCA CACCAGAGAG CTCTCACGAG 
GGGCTGGTGA CCGACCCCCA CAGCCCTGCC CGCTTCCGCG TGCTGGGCAC TCTCTCCAAC 
TCCCGTGACT TCCTGCGGCA CTTCGGCTGC CCTGTCGGCT CCCCCATGAA CCCAGGGCAG 
CTGTGTGAGG TGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001148106.2
CDS	139..2436
Translation	MNVALQELGAGSNMVEYKRATLRDEDAPETPVEGGASPDAMEVGFQKGTRQLLGSRTQLELVLAGVSLLLAALLLGCLVALGVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTQRFYLSCLQVERIEELGAQPLRDLIDKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGLLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW

Target ORF information:

RefSeq Version	XM_001148106.3
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001148106.3

ORF Insert Sequence:

ATGAACGTCG CGCTGCAGGA GCTGGGAGCT GGCAGCAACA TGGTGGAGTA CAAACGGGCC 
ACGCTTCGGG ATGAAGACGC ACCCGAGACC CCCGTAGAGG GCGGGGCCTC CCCGGACGCC 
ATGGAGGTGG GATTCCAGAA GGGGACAAGA CAGCTGTTAG GCTCACGCAC GCAGCTGGAG 
CTGGTCTTAG CAGGTGTCTC TCTACTGCTG GCTGCACTGC TTCTGGGCTG CCTTGTGGCC 
CTAGGGGTCC AGTACCACAG AGACCCATCC CACAGCACCT GCCTTACAGA GGCCTGCATT 
CGAGTGGCTG GAAAAATCCT GGAGTCCCTG GACCGAGGGG TGAGCCCCTG TGAGGACTTT 
TACCAGTTCT CCTGTGGGGG CTGGATTCGG AGGAACCCCC TGCCCGATGG GCGTTCTCGC 
TGGAACACCT TCAACAGCCT CTGGGACCAA AACCAGGCCA TACTGAAGCA CCTGCTTGAA 
AACACCACCT TCAACTCCAG CAGTGAAGCT GAGCGGAAGA CACAGCGCTT CTACCTATCT 
TGCCTACAGG TGGAGCGCAT TGAGGAGCTG GGAGCCCAGC CACTGAGAGA CCTCATTGAC 
AAGATTGGTG GTTGGAACAT TACGGGGCCC TGGGACCAGG ACAACTTTAT GGAGGTGTTG 
AAGGCAGTAG CAGGGACCTA CAGGGCCACC CCATTCTTCA CCGTCTACAT CAGTGCCGAC 
TCTAAGAGTT CCAACAGCAA TGTTATCCAG GTGGACCAGT CTGGGCTCTT TCTGCCCTCT 
CGGGATTACT ACTTAAACAG AACTGCCAAT GAGAAAGTGC TCACTGCCTA TCTGGATTAC 
ATGGAGGAAC TGGGGCTGCT GCTGGGTGGG CGGCCCACCT CCACGAGGGA GCAGATGCAG 
CAGGTGCTGG AGTTGGAGAT ACAGCTGGCC AACATCACAG TGCCCCAGGA CCAGCGGCGC 
GACGAGGAGA AGATCTACCA CAAGATGAGC ATTTCGGAGC TGCAGGCTCT GGCGCCCTCC 
ATGGACTGGC TTGAGTTCCT GTCTTTCTTG CTGTCACCAT TGGAGTTGAG TGACTCTGAG 
CCTGTGGTGG TGTATGGGAT GGATTATTTG CAGCAGGTGT CAGAGCTCAT CAACCGCACG 
GAACCAAGCA TCCTGAACAA TTACCTGATC TGGAACCTGG TGCAAAAGAC AACCTCAAGC 
CTGGACCGAC GCTTTGAGTC TGCACAAGAG AAGCTGCTGG AGACCCTCTA TGGCACTAAG 
AAGTCCTGTG TGCCGAGGTG GCAGACCTGC ATCTCCAACA CGGATGACGC CCTTGGCTTC 
GCTTTGGGGT CCCTCTTCGT GAAGGCCACG TTTGACCGGC AAAGCAAAGA AATTGCAGAG 
GGGATGATCA GCGAAATCCG GACCGCATTT GAGGAGGCCC TGGGACAGCT GGTTTGGATG 
GATGAGAAGA CCCGCCAGGC AGCCAAGGAG AAAGCAGATG CCATCTATGA TATGATTGGT 
TTCCCAGACT TTATCCTGGA GCCCAAAGAG CTGGATGATG TTTATGACGG GTACGAAATT 
TCTGAAGATT CTTTCTTCCA AAACATGTTG AATTTGTACA ACTTCTCTGC CAAGGTTATG 
GCTGACCAGC TCCGCAAGCC TCCCAGCCGA GACCAGTGGA GCATGACCCC CCAGACAGTG 
AATGCCTACT ACCTTCCAAC TAAGAATGAG ATCGTCTTCC CCGCTGGCAT CCTGCAGGCC 
CCCTTCTATG CCCGCAACCA CCCCAAGGCC CTGAACTTTG GTGGCATCGG TGTGGTCATG 
GGCCATGAGT TGACGCATGC CTTTGATGAC CAAGGGCGCG AGTATGACAA AGAAGGGAAC 
CTGCGGCCCT GGTGGCAGAA TGAGTCCCTG GCAGCCTTCC GGAACCACAC GGCCTGCATG 
GAGGAACAGT ACAATCAATA CCAGGTCAAT GGGGAGAGGC TCAACGGCCG CCAGACGCTG 
GGGGAGAACA TTGCTGACAA CGGGGGGCTG AAGGCTGCCT ACAATGCTTA CAAAGCATGG 
CTGAGAAAGC ATGGGGAGGA GCAGCAACTG CCAGCCGTGG GGCTCACCAA CCACCAGCTC 
TTCTTCGTGG GATTTGCCCA GGTGTGGTGC TCGGTCCGCA CACCAGAGAG CTCTCACGAG 
GGGCTGGTGA CCGACCCCCA CAGCCCTGCC CGCTTCCGCG TGCTGGGCAC TCTCTCCAAC 
TCCCGTGACT TCCTGCGGCA CTTCGGCTGC CCTGTCGGCT CCCCCATGAA CCCAGGGCAG 
CTGTGTGAGG TGTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECE2 ( XM_001148031.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001148031.2 Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X3, mRNA.

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CloneID	OPc83043
Clone ID Related Accession (Same CDS sequence)	XM_001148031.2
Accession Version	XM_001148031.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	endothelin-converting enzyme 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036882.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001148031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTCG CGCTGCAGGA GCTGGGAGCT GGCAGCAACG TGGGATTCCA GAAGGGGACA 
AGACAGCTGT TAGGCTCACG CACGCAGCTG GAGCTGGTCT TAGCAGGTGT CTCTCTACTG 
CTGGCTGCAC TGCTTCTGGG CTGCCTTGTG GCCCTAGGGG TCCAGTACCA CAGAGACCCA 
TCCCACAGCA CCTGCCTTAC AGAGGCCTGC ATTCGAGTGG CTGGAAAAAT CCTGGAGTCC 
CTGGACCGAG GGGTGAGCCC CTGTGAGGAC TTTTACCAGT TCTCCTGTGG GGGCTGGATT 
CGGAGGAACC CCCTGCCCGA TGGGCGTTCT CGCTGGAACA CCTTCAACAG CCTCTGGGAC 
CAAAACCAGG CCATACTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC CAGCAGTGAA 
GCTGAGCGGA AGACACAGCG CTTCTACCTA TCTTGCCTAC AGGTGGAGCG CATTGAGGAG 
CTGGGAGCCC AGCCACTGAG AGACCTCATT GACAAGATTG GTGGTTGGAA CATTACGGGG 
CCCTGGGACC AGGACAACTT TATGGAGGTG TTGAAGGCAG TAGCAGGGAC CTACAGGGCC 
ACCCCATTCT TCACCGTCTA CATCAGTGCC GACTCTAAGA GTTCCAACAG CAATGTTATC 
CAGGTGGACC AGTCTGGGCT CTTTCTGCCC TCTCGGGATT ACTACTTAAA CAGAACTGCC 
AATGAGAAAG TGCTCACTGC CTATCTGGAT TACATGGAGG AACTGGGGCT GCTGCTGGGT 
GGGCGGCCCA CCTCCACGAG GGAGCAGATG CAGCAGGTGC TGGAGTTGGA GATACAGCTG 
GCCAACATCA CAGTGCCCCA GGACCAGCGG CGCGACGAGG AGAAGATCTA CCACAAGATG 
AGCATTTCGG AGCTGCAGGC TCTGGCGCCC TCCATGGACT GGCTTGAGTT CCTGTCTTTC 
TTGCTGTCAC CATTGGAGTT GAGTGACTCT GAGCCTGTGG TGGTGTATGG GATGGATTAT 
TTGCAGCAGG TGTCAGAGCT CATCAACCGC ACGGAACCAA GCATCCTGAA CAATTACCTG 
ATCTGGAACC TGGTGCAAAA GACAACCTCA AGCCTGGACC GACGCTTTGA GTCTGCACAA 
GAGAAGCTGC TGGAGACCCT CTATGGCACT AAGAAGTCCT GTGTGCCGAG GTGGCAGACC 
TGCATCTCCA ACACGGATGA CGCCCTTGGC TTCGCTTTGG GGTCCCTCTT CGTGAAGGCC 
ACGTTTGACC GGCAAAGCAA AGAAATTGCA GAGGGGATGA TCAGCGAAAT CCGGACCGCA 
TTTGAGGAGG CCCTGGGACA GCTGGTTTGG ATGGATGAGA AGACCCGCCA GGCAGCCAAG 
GAGAAAGCAG ATGCCATCTA TGATATGATT GGTTTCCCAG ACTTTATCCT GGAGCCCAAA 
GAGCTGGATG ATGTTTATGA CGGGTACGAA ATTTCTGAAG ATTCTTTCTT CCAAAACATG 
TTGAATTTGT ACAACTTCTC TGCCAAGGTT ATGGCTGACC AGCTCCGCAA GCCTCCCAGC 
CGAGACCAGT GGAGCATGAC CCCCCAGACA GTGAATGCCT ACTACCTTCC AACTAAGAAT 
GAGATCGTCT TCCCCGCTGG CATCCTGCAG GCCCCCTTCT ATGCCCGCAA CCACCCCAAG 
GCCCTGAACT TTGGTGGCAT CGGTGTGGTC ATGGGCCATG AGTTGACGCA TGCCTTTGAT 
GACCAAGGGC GCGAGTATGA CAAAGAAGGG AACCTGCGGC CCTGGTGGCA GAATGAGTCC 
CTGGCAGCCT TCCGGAACCA CACGGCCTGC ATGGAGGAAC AGTACAATCA ATACCAGGTC 
AATGGGGAGA GGCTCAACGG CCGCCAGACG CTGGGGGAGA ACATTGCTGA CAACGGGGGG 
CTGAAGGCTG CCTACAATGC TTACAAAGCA TGGCTGAGAA AGCATGGGGA GGAGCAGCAA 
CTGCCAGCCG TGGGGCTCAC CAACCACCAG CTCTTCTTCG TGGGATTTGC CCAGGTGTGG 
TGCTCGGTCC GCACACCAGA GAGCTCTCAC GAGGGGCTGG TGACCGACCC CCACAGCCCT 
GCCCGCTTCC GCGTGCTGGG CACTCTCTCC AACTCCCGTG ACTTCCTGCG GCACTTCGGC 
TGCCCTGTCG GCTCCCCCAT GAACCCAGGG CAGCTGTGTG AGGTGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001148031.2
CDS	139..2349
Translation	MNVALQELGAGSNVGFQKGTRQLLGSRTQLELVLAGVSLLLAALLLGCLVALGVQYHRDPSHSTCLTEACIRVAGKILESLDRGVSPCEDFYQFSCGGWIRRNPLPDGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTQRFYLSCLQVERIEELGAQPLRDLIDKIGGWNITGPWDQDNFMEVLKAVAGTYRATPFFTVYISADSKSSNSNVIQVDQSGLFLPSRDYYLNRTANEKVLTAYLDYMEELGLLLGGRPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPSMDWLEFLSFLLSPLELSDSEPVVVYGMDYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDRRFESAQEKLLETLYGTKKSCVPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMISEIRTAFEEALGQLVWMDEKTRQAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEISEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSMTPQTVNAYYLPTKNEIVFPAGILQAPFYARNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWWQNESLAAFRNHTACMEEQYNQYQVNGERLNGRQTLGENIADNGGLKAAYNAYKAWLRKHGEEQQLPAVGLTNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW

Target ORF information:

RefSeq Version	XM_001148031.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes endothelin converting enzyme 2 (ECE2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001148031.2

ORF Insert Sequence:

ATGAACGTCG CGCTGCAGGA GCTGGGAGCT GGCAGCAACG TGGGATTCCA GAAGGGGACA 
AGACAGCTGT TAGGCTCACG CACGCAGCTG GAGCTGGTCT TAGCAGGTGT CTCTCTACTG 
CTGGCTGCAC TGCTTCTGGG CTGCCTTGTG GCCCTAGGGG TCCAGTACCA CAGAGACCCA 
TCCCACAGCA CCTGCCTTAC AGAGGCCTGC ATTCGAGTGG CTGGAAAAAT CCTGGAGTCC 
CTGGACCGAG GGGTGAGCCC CTGTGAGGAC TTTTACCAGT TCTCCTGTGG GGGCTGGATT 
CGGAGGAACC CCCTGCCCGA TGGGCGTTCT CGCTGGAACA CCTTCAACAG CCTCTGGGAC 
CAAAACCAGG CCATACTGAA GCACCTGCTT GAAAACACCA CCTTCAACTC CAGCAGTGAA 
GCTGAGCGGA AGACACAGCG CTTCTACCTA TCTTGCCTAC AGGTGGAGCG CATTGAGGAG 
CTGGGAGCCC AGCCACTGAG AGACCTCATT GACAAGATTG GTGGTTGGAA CATTACGGGG 
CCCTGGGACC AGGACAACTT TATGGAGGTG TTGAAGGCAG TAGCAGGGAC CTACAGGGCC 
ACCCCATTCT TCACCGTCTA CATCAGTGCC GACTCTAAGA GTTCCAACAG CAATGTTATC 
CAGGTGGACC AGTCTGGGCT CTTTCTGCCC TCTCGGGATT ACTACTTAAA CAGAACTGCC 
AATGAGAAAG TGCTCACTGC CTATCTGGAT TACATGGAGG AACTGGGGCT GCTGCTGGGT 
GGGCGGCCCA CCTCCACGAG GGAGCAGATG CAGCAGGTGC TGGAGTTGGA GATACAGCTG 
GCCAACATCA CAGTGCCCCA GGACCAGCGG CGCGACGAGG AGAAGATCTA CCACAAGATG 
AGCATTTCGG AGCTGCAGGC TCTGGCGCCC TCCATGGACT GGCTTGAGTT CCTGTCTTTC 
TTGCTGTCAC CATTGGAGTT GAGTGACTCT GAGCCTGTGG TGGTGTATGG GATGGATTAT 
TTGCAGCAGG TGTCAGAGCT CATCAACCGC ACGGAACCAA GCATCCTGAA CAATTACCTG 
ATCTGGAACC TGGTGCAAAA GACAACCTCA AGCCTGGACC GACGCTTTGA GTCTGCACAA 
GAGAAGCTGC TGGAGACCCT CTATGGCACT AAGAAGTCCT GTGTGCCGAG GTGGCAGACC 
TGCATCTCCA ACACGGATGA CGCCCTTGGC TTCGCTTTGG GGTCCCTCTT CGTGAAGGCC 
ACGTTTGACC GGCAAAGCAA AGAAATTGCA GAGGGGATGA TCAGCGAAAT CCGGACCGCA 
TTTGAGGAGG CCCTGGGACA GCTGGTTTGG ATGGATGAGA AGACCCGCCA GGCAGCCAAG 
GAGAAAGCAG ATGCCATCTA TGATATGATT GGTTTCCCAG ACTTTATCCT GGAGCCCAAA 
GAGCTGGATG ATGTTTATGA CGGGTACGAA ATTTCTGAAG ATTCTTTCTT CCAAAACATG 
TTGAATTTGT ACAACTTCTC TGCCAAGGTT ATGGCTGACC AGCTCCGCAA GCCTCCCAGC 
CGAGACCAGT GGAGCATGAC CCCCCAGACA GTGAATGCCT ACTACCTTCC AACTAAGAAT 
GAGATCGTCT TCCCCGCTGG CATCCTGCAG GCCCCCTTCT ATGCCCGCAA CCACCCCAAG 
GCCCTGAACT TTGGTGGCAT CGGTGTGGTC ATGGGCCATG AGTTGACGCA TGCCTTTGAT 
GACCAAGGGC GCGAGTATGA CAAAGAAGGG AACCTGCGGC CCTGGTGGCA GAATGAGTCC 
CTGGCAGCCT TCCGGAACCA CACGGCCTGC ATGGAGGAAC AGTACAATCA ATACCAGGTC 
AATGGGGAGA GGCTCAACGG CCGCCAGACG CTGGGGGAGA ACATTGCTGA CAACGGGGGG 
CTGAAGGCTG CCTACAATGC TTACAAAGCA TGGCTGAGAA AGCATGGGGA GGAGCAGCAA 
CTGCCAGCCG TGGGGCTCAC CAACCACCAG CTCTTCTTCG TGGGATTTGC CCAGGTGTGG 
TGCTCGGTCC GCACACCAGA GAGCTCTCAC GAGGGGCTGG TGACCGACCC CCACAGCCCT 
GCCCGCTTCC GCGTGCTGGG CACTCTCTCC AACTCCCGTG ACTTCCTGCG GCACTTCGGC 
TGCCCTGTCG GCTCCCCCAT GAACCCAGGG CAGCTGTGTG AGGTGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ECE2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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