LOC4325131 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC4325131
Entrez Gene ID	4325131
Full Name	callose synthase 12
Gene Type	protein-coding
Organism	Oryza sativa Japonica Group(Japanese rice)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015761145.1	XP_015616631.1	callose synthase 12
XM_015761145.2	XP_015616631.1	callose synthase 12
XM_015761139.2	XP_015616625.1	callose synthase 12
XM_015761151.2	XP_015616637.1	callose synthase 12
XM_015761139.1	XP_015616625.1	callose synthase 12
XM_015761151.1	XP_015616637.1	callose synthase 12

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	GSL1	NP_567278.1
Arabidopsis thaliana (thale cress)	GSL05	NP_192264.1
Oryza sativa (rice)	Os01g0754200	NP_001044278.1

Gene Ontology

The following LOC4325131 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC4325131 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOa00870	XM_015761139.2 Latest version!	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOa00870	XM_015761151.2 Latest version!	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOa00870	XM_015761145.2 Latest version!	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OOa00870	XM_015761145.1	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOa00870	XM_015761139.1	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOa00870	XM_015761151.1	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC4325131 ( XM_015761145.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015616631.1 Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOa00870
Clone ID Related Accession (Same CDS sequence)	XM_015761145.1 , XM_015761145.2 , XM_015761139.2 , XM_015761151.2 , XM_015761139.1 , XM_015761151.1
Accession Version	XM_015761145.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	callose synthase 12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379174.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015616631.1
CDS	259..5631
Translation	MSLLRNRRAAAAAGSGEQTVVQAAYNIIPIQDVVMHGDHPSLQVPEVRAAVEALSHASDFPAPPLARVWDPHRADIFDWLGATFGFQADNVRNQREHLVLLLANAQLRAAPGFPKDHPIDVLHLTVARGIRRKLLKNYTSWCAYLGQKRHFRVPSGGGGGRRTGAATGNDVRMDLLYTALYLLIWGEAANLRFMPECLCYIFHYMALDLHHVVEQSIDIETGRPAMPAVCGEDAFLIRVVTPIYNVLKNEVEASRNGTKPHSAWRNYDDVNEYFWSRRVFKRLRWPLDPSRSFFVEPGKTGRIGKTGFVEQRSFWNVYRSFDRVWVMHILFFQAAMIVAWDGKTPWVSLRFRDIQVRVLSVFITWGGLRFVQAMLDAGTQYSLVSRETKTVAVRMVLKVLVAAGWTITFSVLYKRMWDQRWRDRRWSFAANTRVLNYLEAAAVFVIPQVLAIVLFIIPWIRNFLEKTNWKILYVLTWWFQTRTFVGRGLREGLIDNIKYSIFWVCLLVSKFSFSYFLQIKPMVGPTKVIFKLHDIKRNWFEFMPHTERLAVIILWLPVIIIYLMDIQIWYAVFSSLTGALIGLFSHLGEIRSVEQLRLRFQFFASAMQFNLMPEEHLDTVHGGIRSKFYDAINRLKLRYGFGRPYRKIEANEVEAKRFALVWNEIIQTFREEDIISDKELGLLELPAVVWRIRVVRWPCLLLKNELLLALSQAAELVADDRTHWNKICNNEYRRCAVIEAYDSIRHLLLEIIKERTNEHIIVNQLFLAFDGAMEYGKFTEEYRLTLLPQIHKYVISLVEQLLLKDKDQIKIVRTLQDLYDLAVHDFPKIKKDFEQLRREGLALSRPTESQLLFQDAIKCPDDNDVSFYKQVRRLHTILTSRDSMDDVPKNPEARRRITFFSNSLFMNMPRAPTVQRMMAFSVLTPYYNEDVLYNKDQLRRENEDGISILFYLQKIYEDDWKNFLERMQREGMASDDGIWAGKFQDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDNASEVEITEGTKQLASFGSVQYENDVYPMNGGLSQRPRRRLERGTSTVSQLFKGQEDGAAIMKYTYVVACQIYGNQKKAKDQRAEDILTLMKKNDALRVAYVDEVHPEIGDTQYYSVLVKFDPVLQREVEIYRIRLPGQLKLGEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEQYDYYHGSQKPTLLGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRLWFLTRGGISKASRVINISEDIFAGFNCTLRGGNVSHHEYIQVGKGRDVGLNQISMFEAKVSSGNGEQTLSRDIYRLGHRLDFFRSLSVFYTTVGFYFNTMMVVLTVYTFVWGRLYLALSGLEAGIQGSANATNNKALGAVLNQQFVIQLGLFTALPMIIENSLEQGFLPAVWDFFTMQMMFSSVFYTFSMGTKSHYYGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGIVLTVYAAHSVIARDTLVYIIMMISSWFLVVSWIMAPFAFNPSGFDWLKTVYDFDDFMNWIWYPGSIFSKAEHSWEVWWFEEQDHLRTTGLWGKILEILLDLRYFFFQYGVVYQLKIANESRSIAVYLLSWICVAVIFGIFVLMSYARDKYAAKQHLYYRVIQSGVIILAVLVLIIFLKFTKFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWASVVSVARLYEILLGVFVMAPVAFFSWLPGFQEMQTRVLFNEAFSRGLQISRILAGKKTIAV

Target ORF information:

RefSeq Version	XM_015761145.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015761145.1

ORF Insert Sequence:

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4325131 ( XM_015761145.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015616631.1 Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X2, mRNA.

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CloneID	OOa00870
Clone ID Related Accession (Same CDS sequence)	XM_015761145.1 , XM_015761145.2 , XM_015761139.2 , XM_015761151.2 , XM_015761139.1 , XM_015761151.1
Accession Version	XM_015761145.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	callose synthase 12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029256.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015761145.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015616631.1
CDS	352..5724
Translation	MSLLRNRRAAAAAGSGEQTVVQAAYNIIPIQDVVMHGDHPSLQVPEVRAAVEALSHASDFPAPPLARVWDPHRADIFDWLGATFGFQADNVRNQREHLVLLLANAQLRAAPGFPKDHPIDVLHLTVARGIRRKLLKNYTSWCAYLGQKRHFRVPSGGGGGRRTGAATGNDVRMDLLYTALYLLIWGEAANLRFMPECLCYIFHYMALDLHHVVEQSIDIETGRPAMPAVCGEDAFLIRVVTPIYNVLKNEVEASRNGTKPHSAWRNYDDVNEYFWSRRVFKRLRWPLDPSRSFFVEPGKTGRIGKTGFVEQRSFWNVYRSFDRVWVMHILFFQAAMIVAWDGKTPWVSLRFRDIQVRVLSVFITWGGLRFVQAMLDAGTQYSLVSRETKTVAVRMVLKVLVAAGWTITFSVLYKRMWDQRWRDRRWSFAANTRVLNYLEAAAVFVIPQVLAIVLFIIPWIRNFLEKTNWKILYVLTWWFQTRTFVGRGLREGLIDNIKYSIFWVCLLVSKFSFSYFLQIKPMVGPTKVIFKLHDIKRNWFEFMPHTERLAVIILWLPVIIIYLMDIQIWYAVFSSLTGALIGLFSHLGEIRSVEQLRLRFQFFASAMQFNLMPEEHLDTVHGGIRSKFYDAINRLKLRYGFGRPYRKIEANEVEAKRFALVWNEIIQTFREEDIISDKELGLLELPAVVWRIRVVRWPCLLLKNELLLALSQAAELVADDRTHWNKICNNEYRRCAVIEAYDSIRHLLLEIIKERTNEHIIVNQLFLAFDGAMEYGKFTEEYRLTLLPQIHKYVISLVEQLLLKDKDQIKIVRTLQDLYDLAVHDFPKIKKDFEQLRREGLALSRPTESQLLFQDAIKCPDDNDVSFYKQVRRLHTILTSRDSMDDVPKNPEARRRITFFSNSLFMNMPRAPTVQRMMAFSVLTPYYNEDVLYNKDQLRRENEDGISILFYLQKIYEDDWKNFLERMQREGMASDDGIWAGKFQDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDNASEVEITEGTKQLASFGSVQYENDVYPMNGGLSQRPRRRLERGTSTVSQLFKGQEDGAAIMKYTYVVACQIYGNQKKAKDQRAEDILTLMKKNDALRVAYVDEVHPEIGDTQYYSVLVKFDPVLQREVEIYRIRLPGQLKLGEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEQYDYYHGSQKPTLLGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRLWFLTRGGISKASRVINISEDIFAGFNCTLRGGNVSHHEYIQVGKGRDVGLNQISMFEAKVSSGNGEQTLSRDIYRLGHRLDFFRSLSVFYTTVGFYFNTMMVVLTVYTFVWGRLYLALSGLEAGIQGSANATNNKALGAVLNQQFVIQLGLFTALPMIIENSLEQGFLPAVWDFFTMQMMFSSVFYTFSMGTKSHYYGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGIVLTVYAAHSVIARDTLVYIIMMISSWFLVVSWIMAPFAFNPSGFDWLKTVYDFDDFMNWIWYPGSIFSKAEHSWEVWWFEEQDHLRTTGLWGKILEILLDLRYFFFQYGVVYQLKIANESRSIAVYLLSWICVAVIFGIFVLMSYARDKYAAKQHLYYRVIQSGVIILAVLVLIIFLKFTKFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWASVVSVARLYEILLGVFVMAPVAFFSWLPGFQEMQTRVLFNEAFSRGLQISRILAGKKTIAV

Target ORF information:

RefSeq Version	XM_015761145.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015761145.2

ORF Insert Sequence:

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4325131 ( XM_015761139.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015616625.1 Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOa00870
Clone ID Related Accession (Same CDS sequence)	XM_015761145.1 , XM_015761145.2 , XM_015761139.2 , XM_015761151.2 , XM_015761139.1 , XM_015761151.1
Accession Version	XM_015761139.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	callose synthase 12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029256.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015761139.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015616625.1
CDS	277..5649
Translation	MSLLRNRRAAAAAGSGEQTVVQAAYNIIPIQDVVMHGDHPSLQVPEVRAAVEALSHASDFPAPPLARVWDPHRADIFDWLGATFGFQADNVRNQREHLVLLLANAQLRAAPGFPKDHPIDVLHLTVARGIRRKLLKNYTSWCAYLGQKRHFRVPSGGGGGRRTGAATGNDVRMDLLYTALYLLIWGEAANLRFMPECLCYIFHYMALDLHHVVEQSIDIETGRPAMPAVCGEDAFLIRVVTPIYNVLKNEVEASRNGTKPHSAWRNYDDVNEYFWSRRVFKRLRWPLDPSRSFFVEPGKTGRIGKTGFVEQRSFWNVYRSFDRVWVMHILFFQAAMIVAWDGKTPWVSLRFRDIQVRVLSVFITWGGLRFVQAMLDAGTQYSLVSRETKTVAVRMVLKVLVAAGWTITFSVLYKRMWDQRWRDRRWSFAANTRVLNYLEAAAVFVIPQVLAIVLFIIPWIRNFLEKTNWKILYVLTWWFQTRTFVGRGLREGLIDNIKYSIFWVCLLVSKFSFSYFLQIKPMVGPTKVIFKLHDIKRNWFEFMPHTERLAVIILWLPVIIIYLMDIQIWYAVFSSLTGALIGLFSHLGEIRSVEQLRLRFQFFASAMQFNLMPEEHLDTVHGGIRSKFYDAINRLKLRYGFGRPYRKIEANEVEAKRFALVWNEIIQTFREEDIISDKELGLLELPAVVWRIRVVRWPCLLLKNELLLALSQAAELVADDRTHWNKICNNEYRRCAVIEAYDSIRHLLLEIIKERTNEHIIVNQLFLAFDGAMEYGKFTEEYRLTLLPQIHKYVISLVEQLLLKDKDQIKIVRTLQDLYDLAVHDFPKIKKDFEQLRREGLALSRPTESQLLFQDAIKCPDDNDVSFYKQVRRLHTILTSRDSMDDVPKNPEARRRITFFSNSLFMNMPRAPTVQRMMAFSVLTPYYNEDVLYNKDQLRRENEDGISILFYLQKIYEDDWKNFLERMQREGMASDDGIWAGKFQDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDNASEVEITEGTKQLASFGSVQYENDVYPMNGGLSQRPRRRLERGTSTVSQLFKGQEDGAAIMKYTYVVACQIYGNQKKAKDQRAEDILTLMKKNDALRVAYVDEVHPEIGDTQYYSVLVKFDPVLQREVEIYRIRLPGQLKLGEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEQYDYYHGSQKPTLLGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRLWFLTRGGISKASRVINISEDIFAGFNCTLRGGNVSHHEYIQVGKGRDVGLNQISMFEAKVSSGNGEQTLSRDIYRLGHRLDFFRSLSVFYTTVGFYFNTMMVVLTVYTFVWGRLYLALSGLEAGIQGSANATNNKALGAVLNQQFVIQLGLFTALPMIIENSLEQGFLPAVWDFFTMQMMFSSVFYTFSMGTKSHYYGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGIVLTVYAAHSVIARDTLVYIIMMISSWFLVVSWIMAPFAFNPSGFDWLKTVYDFDDFMNWIWYPGSIFSKAEHSWEVWWFEEQDHLRTTGLWGKILEILLDLRYFFFQYGVVYQLKIANESRSIAVYLLSWICVAVIFGIFVLMSYARDKYAAKQHLYYRVIQSGVIILAVLVLIIFLKFTKFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWASVVSVARLYEILLGVFVMAPVAFFSWLPGFQEMQTRVLFNEAFSRGLQISRILAGKKTIAV

Target ORF information:

RefSeq Version	XM_015761139.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015761139.2

ORF Insert Sequence:

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4325131 ( XM_015761151.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015616637.1 Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X3, mRNA.

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CloneID	OOa00870
Clone ID Related Accession (Same CDS sequence)	XM_015761145.1 , XM_015761145.2 , XM_015761139.2 , XM_015761151.2 , XM_015761139.1 , XM_015761151.1
Accession Version	XM_015761151.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	callose synthase 12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029256.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015761151.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015616637.1
CDS	337..5709
Translation	MSLLRNRRAAAAAGSGEQTVVQAAYNIIPIQDVVMHGDHPSLQVPEVRAAVEALSHASDFPAPPLARVWDPHRADIFDWLGATFGFQADNVRNQREHLVLLLANAQLRAAPGFPKDHPIDVLHLTVARGIRRKLLKNYTSWCAYLGQKRHFRVPSGGGGGRRTGAATGNDVRMDLLYTALYLLIWGEAANLRFMPECLCYIFHYMALDLHHVVEQSIDIETGRPAMPAVCGEDAFLIRVVTPIYNVLKNEVEASRNGTKPHSAWRNYDDVNEYFWSRRVFKRLRWPLDPSRSFFVEPGKTGRIGKTGFVEQRSFWNVYRSFDRVWVMHILFFQAAMIVAWDGKTPWVSLRFRDIQVRVLSVFITWGGLRFVQAMLDAGTQYSLVSRETKTVAVRMVLKVLVAAGWTITFSVLYKRMWDQRWRDRRWSFAANTRVLNYLEAAAVFVIPQVLAIVLFIIPWIRNFLEKTNWKILYVLTWWFQTRTFVGRGLREGLIDNIKYSIFWVCLLVSKFSFSYFLQIKPMVGPTKVIFKLHDIKRNWFEFMPHTERLAVIILWLPVIIIYLMDIQIWYAVFSSLTGALIGLFSHLGEIRSVEQLRLRFQFFASAMQFNLMPEEHLDTVHGGIRSKFYDAINRLKLRYGFGRPYRKIEANEVEAKRFALVWNEIIQTFREEDIISDKELGLLELPAVVWRIRVVRWPCLLLKNELLLALSQAAELVADDRTHWNKICNNEYRRCAVIEAYDSIRHLLLEIIKERTNEHIIVNQLFLAFDGAMEYGKFTEEYRLTLLPQIHKYVISLVEQLLLKDKDQIKIVRTLQDLYDLAVHDFPKIKKDFEQLRREGLALSRPTESQLLFQDAIKCPDDNDVSFYKQVRRLHTILTSRDSMDDVPKNPEARRRITFFSNSLFMNMPRAPTVQRMMAFSVLTPYYNEDVLYNKDQLRRENEDGISILFYLQKIYEDDWKNFLERMQREGMASDDGIWAGKFQDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDNASEVEITEGTKQLASFGSVQYENDVYPMNGGLSQRPRRRLERGTSTVSQLFKGQEDGAAIMKYTYVVACQIYGNQKKAKDQRAEDILTLMKKNDALRVAYVDEVHPEIGDTQYYSVLVKFDPVLQREVEIYRIRLPGQLKLGEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEQYDYYHGSQKPTLLGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRLWFLTRGGISKASRVINISEDIFAGFNCTLRGGNVSHHEYIQVGKGRDVGLNQISMFEAKVSSGNGEQTLSRDIYRLGHRLDFFRSLSVFYTTVGFYFNTMMVVLTVYTFVWGRLYLALSGLEAGIQGSANATNNKALGAVLNQQFVIQLGLFTALPMIIENSLEQGFLPAVWDFFTMQMMFSSVFYTFSMGTKSHYYGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGIVLTVYAAHSVIARDTLVYIIMMISSWFLVVSWIMAPFAFNPSGFDWLKTVYDFDDFMNWIWYPGSIFSKAEHSWEVWWFEEQDHLRTTGLWGKILEILLDLRYFFFQYGVVYQLKIANESRSIAVYLLSWICVAVIFGIFVLMSYARDKYAAKQHLYYRVIQSGVIILAVLVLIIFLKFTKFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWASVVSVARLYEILLGVFVMAPVAFFSWLPGFQEMQTRVLFNEAFSRGLQISRILAGKKTIAV

Target ORF information:

RefSeq Version	XM_015761151.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015761151.2

ORF Insert Sequence:

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4325131 ( XM_015761139.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015616625.1 Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X1, mRNA.

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CloneID	OOa00870
Clone ID Related Accession (Same CDS sequence)	XM_015761145.1 , XM_015761145.2 , XM_015761139.2 , XM_015761151.2 , XM_015761139.1 , XM_015761151.1
Accession Version	XM_015761139.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	callose synthase 12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379174.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015616625.1
CDS	236..5608
Translation	MSLLRNRRAAAAAGSGEQTVVQAAYNIIPIQDVVMHGDHPSLQVPEVRAAVEALSHASDFPAPPLARVWDPHRADIFDWLGATFGFQADNVRNQREHLVLLLANAQLRAAPGFPKDHPIDVLHLTVARGIRRKLLKNYTSWCAYLGQKRHFRVPSGGGGGRRTGAATGNDVRMDLLYTALYLLIWGEAANLRFMPECLCYIFHYMALDLHHVVEQSIDIETGRPAMPAVCGEDAFLIRVVTPIYNVLKNEVEASRNGTKPHSAWRNYDDVNEYFWSRRVFKRLRWPLDPSRSFFVEPGKTGRIGKTGFVEQRSFWNVYRSFDRVWVMHILFFQAAMIVAWDGKTPWVSLRFRDIQVRVLSVFITWGGLRFVQAMLDAGTQYSLVSRETKTVAVRMVLKVLVAAGWTITFSVLYKRMWDQRWRDRRWSFAANTRVLNYLEAAAVFVIPQVLAIVLFIIPWIRNFLEKTNWKILYVLTWWFQTRTFVGRGLREGLIDNIKYSIFWVCLLVSKFSFSYFLQIKPMVGPTKVIFKLHDIKRNWFEFMPHTERLAVIILWLPVIIIYLMDIQIWYAVFSSLTGALIGLFSHLGEIRSVEQLRLRFQFFASAMQFNLMPEEHLDTVHGGIRSKFYDAINRLKLRYGFGRPYRKIEANEVEAKRFALVWNEIIQTFREEDIISDKELGLLELPAVVWRIRVVRWPCLLLKNELLLALSQAAELVADDRTHWNKICNNEYRRCAVIEAYDSIRHLLLEIIKERTNEHIIVNQLFLAFDGAMEYGKFTEEYRLTLLPQIHKYVISLVEQLLLKDKDQIKIVRTLQDLYDLAVHDFPKIKKDFEQLRREGLALSRPTESQLLFQDAIKCPDDNDVSFYKQVRRLHTILTSRDSMDDVPKNPEARRRITFFSNSLFMNMPRAPTVQRMMAFSVLTPYYNEDVLYNKDQLRRENEDGISILFYLQKIYEDDWKNFLERMQREGMASDDGIWAGKFQDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDNASEVEITEGTKQLASFGSVQYENDVYPMNGGLSQRPRRRLERGTSTVSQLFKGQEDGAAIMKYTYVVACQIYGNQKKAKDQRAEDILTLMKKNDALRVAYVDEVHPEIGDTQYYSVLVKFDPVLQREVEIYRIRLPGQLKLGEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEQYDYYHGSQKPTLLGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRLWFLTRGGISKASRVINISEDIFAGFNCTLRGGNVSHHEYIQVGKGRDVGLNQISMFEAKVSSGNGEQTLSRDIYRLGHRLDFFRSLSVFYTTVGFYFNTMMVVLTVYTFVWGRLYLALSGLEAGIQGSANATNNKALGAVLNQQFVIQLGLFTALPMIIENSLEQGFLPAVWDFFTMQMMFSSVFYTFSMGTKSHYYGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGIVLTVYAAHSVIARDTLVYIIMMISSWFLVVSWIMAPFAFNPSGFDWLKTVYDFDDFMNWIWYPGSIFSKAEHSWEVWWFEEQDHLRTTGLWGKILEILLDLRYFFFQYGVVYQLKIANESRSIAVYLLSWICVAVIFGIFVLMSYARDKYAAKQHLYYRVIQSGVIILAVLVLIIFLKFTKFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWASVVSVARLYEILLGVFVMAPVAFFSWLPGFQEMQTRVLFNEAFSRGLQISRILAGKKTIAV

Target ORF information:

RefSeq Version	XM_015761139.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015761139.1

ORF Insert Sequence:

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4325131 ( XM_015761151.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015616637.1 Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X3, mRNA.

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CloneID	OOa00870
Clone ID Related Accession (Same CDS sequence)	XM_015761145.1 , XM_015761145.2 , XM_015761139.2 , XM_015761151.2 , XM_015761139.1 , XM_015761151.1
Accession Version	XM_015761151.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5373bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	callose synthase 12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379174.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015616637.1
CDS	244..5616
Translation	MSLLRNRRAAAAAGSGEQTVVQAAYNIIPIQDVVMHGDHPSLQVPEVRAAVEALSHASDFPAPPLARVWDPHRADIFDWLGATFGFQADNVRNQREHLVLLLANAQLRAAPGFPKDHPIDVLHLTVARGIRRKLLKNYTSWCAYLGQKRHFRVPSGGGGGRRTGAATGNDVRMDLLYTALYLLIWGEAANLRFMPECLCYIFHYMALDLHHVVEQSIDIETGRPAMPAVCGEDAFLIRVVTPIYNVLKNEVEASRNGTKPHSAWRNYDDVNEYFWSRRVFKRLRWPLDPSRSFFVEPGKTGRIGKTGFVEQRSFWNVYRSFDRVWVMHILFFQAAMIVAWDGKTPWVSLRFRDIQVRVLSVFITWGGLRFVQAMLDAGTQYSLVSRETKTVAVRMVLKVLVAAGWTITFSVLYKRMWDQRWRDRRWSFAANTRVLNYLEAAAVFVIPQVLAIVLFIIPWIRNFLEKTNWKILYVLTWWFQTRTFVGRGLREGLIDNIKYSIFWVCLLVSKFSFSYFLQIKPMVGPTKVIFKLHDIKRNWFEFMPHTERLAVIILWLPVIIIYLMDIQIWYAVFSSLTGALIGLFSHLGEIRSVEQLRLRFQFFASAMQFNLMPEEHLDTVHGGIRSKFYDAINRLKLRYGFGRPYRKIEANEVEAKRFALVWNEIIQTFREEDIISDKELGLLELPAVVWRIRVVRWPCLLLKNELLLALSQAAELVADDRTHWNKICNNEYRRCAVIEAYDSIRHLLLEIIKERTNEHIIVNQLFLAFDGAMEYGKFTEEYRLTLLPQIHKYVISLVEQLLLKDKDQIKIVRTLQDLYDLAVHDFPKIKKDFEQLRREGLALSRPTESQLLFQDAIKCPDDNDVSFYKQVRRLHTILTSRDSMDDVPKNPEARRRITFFSNSLFMNMPRAPTVQRMMAFSVLTPYYNEDVLYNKDQLRRENEDGISILFYLQKIYEDDWKNFLERMQREGMASDDGIWAGKFQDLRLWASYRGQTLARTVRGMMYYYRALKMLAFLDNASEVEITEGTKQLASFGSVQYENDVYPMNGGLSQRPRRRLERGTSTVSQLFKGQEDGAAIMKYTYVVACQIYGNQKKAKDQRAEDILTLMKKNDALRVAYVDEVHPEIGDTQYYSVLVKFDPVLQREVEIYRIRLPGQLKLGEGKPENQNHAIIFTRGDAVQTIDMNQDNYFEEALKMRNLLEQYDYYHGSQKPTLLGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLANPLKVRMHYGHPDVFDRLWFLTRGGISKASRVINISEDIFAGFNCTLRGGNVSHHEYIQVGKGRDVGLNQISMFEAKVSSGNGEQTLSRDIYRLGHRLDFFRSLSVFYTTVGFYFNTMMVVLTVYTFVWGRLYLALSGLEAGIQGSANATNNKALGAVLNQQFVIQLGLFTALPMIIENSLEQGFLPAVWDFFTMQMMFSSVFYTFSMGTKSHYYGRTILHGGAKYRATGRGFVVQHKSFAENYRLYARSHFIKAIELGIVLTVYAAHSVIARDTLVYIIMMISSWFLVVSWIMAPFAFNPSGFDWLKTVYDFDDFMNWIWYPGSIFSKAEHSWEVWWFEEQDHLRTTGLWGKILEILLDLRYFFFQYGVVYQLKIANESRSIAVYLLSWICVAVIFGIFVLMSYARDKYAAKQHLYYRVIQSGVIILAVLVLIIFLKFTKFQIIDIFTSLLAFIPTGWGLISIAQVIRPFIESTVVWASVVSVARLYEILLGVFVMAPVAFFSWLPGFQEMQTRVLFNEAFSRGLQISRILAGKKTIAV

Target ORF information:

RefSeq Version	XM_015761151.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group callose synthase 12 (LOC4325131), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015761151.1

ORF Insert Sequence:

ATGAGCTTGC TGCGGAACCG CCGCGCCGCG GCGGCGGCGG GTTCGGGGGA GCAGACGGTG 
GTGCAGGCGG CGTACAACAT CATCCCGATC CAGGACGTGG TGATGCACGG GGACCACCCG 
TCGCTGCAGG TGCCGGAAGT GCGGGCGGCG GTGGAGGCGC TGTCGCACGC GTCGGACTTC 
CCGGCGCCGC CGCTGGCCCG CGTCTGGGAC CCGCACCGCG CCGACATCTT CGACTGGCTC 
GGCGCCACCT TCGGGTTCCA GGCGGACAAC GTCCGGAACC AGCGGGAGCA CCTGGTGCTC 
CTCCTCGCCA ACGCGCAGCT CCGCGCCGCG CCGGGATTCC CCAAGGACCA CCCCATCGAC 
GTCCTCCACC TCACCGTCGC CCGCGGCATC CGCAGGAAGC TCCTCAAGAA CTACACCTCC 
TGGTGCGCCT ACCTCGGCCA GAAGCGCCAC TTCCGCGTGC CCAGCGGCGG CGGCGGCGGC 
CGCCGCACCG GCGCCGCCAC CGGCAACGAC GTCCGCATGG ACCTCCTCTA CACGGCGCTG 
TACCTGCTCA TCTGGGGGGA GGCCGCCAAC CTGAGGTTCA TGCCGGAGTG CCTCTGCTAC 
ATCTTCCACT ACATGGCCCT CGACCTCCAC CACGTCGTCG AGCAGTCCAT CGACATCGAG 
ACAGGGCGGC CTGCGATGCC CGCCGTGTGC GGCGAGGACG CCTTCCTCAT CCGCGTGGTG 
ACTCCGATCT ACAACGTCCT CAAGAACGAG GTGGAGGCCA GCCGGAACGG GACCAAGCCG 
CACTCGGCGT GGAGGAATTA CGACGATGTC AACGAGTACT TCTGGAGCCG ACGAGTTTTC 
AAGCGGCTCC GGTGGCCGCT GGATCCATCG AGGAGCTTCT TTGTGGAGCC TGGAAAGACT 
GGGCGCATTG GCAAGACTGG CTTTGTTGAG CAGCGGTCAT TCTGGAATGT CTACCGAAGT 
TTCGATAGGG TATGGGTGAT GCATATTCTG TTCTTTCAAG CTGCGATGAT TGTTGCATGG 
GATGGTAAAA CCCCATGGGT TAGCCTCAGA TTCCGGGACA TCCAGGTCCG GGTGCTGTCT 
GTGTTCATCA CTTGGGGTGG ACTGCGCTTT GTTCAGGCAA TGCTTGATGC GGGTACTCAG 
TACAGTCTCG TGTCGAGGGA GACAAAAACA GTGGCTGTTC GGATGGTGCT CAAGGTGCTT 
GTTGCTGCAG GGTGGACAAT CACATTCAGT GTGCTCTATA AGCGAATGTG GGATCAGCGG 
TGGCGAGATC GCAGGTGGTC CTTTGCTGCC AATACCAGAG TGCTGAATTA CCTTGAGGCA 
GCTGCTGTGT TTGTCATCCC GCAAGTGCTT GCGATTGTGC TCTTCATCAT CCCTTGGATT 
CGAAATTTTC TGGAGAAAAC CAATTGGAAA ATTCTATATG TGCTGACCTG GTGGTTCCAA 
ACACGTACAT TTGTTGGCCG TGGTCTGAGG GAAGGTCTCA TTGATAACAT CAAGTACTCC 
ATATTCTGGG TGTGCCTCCT TGTTTCTAAG TTTAGCTTCA GCTACTTTCT CCAGATTAAG 
CCGATGGTGG GGCCAACAAA GGTAATCTTC AAACTCCATG ACATCAAGCG TAACTGGTTT 
GAGTTCATGC CTCACACAGA GCGCCTTGCA GTAATCATCC TGTGGCTCCC AGTTATTATC 
ATTTACCTCA TGGATATCCA GATATGGTAT GCAGTCTTCT CATCACTAAC AGGGGCGCTT 
ATCGGCCTTT TCTCGCATCT GGGGGAGATT CGCAGTGTTG AGCAGCTGCG CTTGAGGTTC 
CAGTTCTTTG CAAGTGCAAT GCAGTTCAAT TTAATGCCAG AGGAGCACCT GGATACCGTT 
CATGGTGGCA TCCGCAGCAA GTTCTATGAT GCAATCAATC GGCTGAAGCT GAGATATGGG 
TTTGGCCGTC CATATAGGAA GATTGAAGCA AACGAGGTGG AAGCTAAGAG GTTTGCACTG 
GTTTGGAATG AGATCATTCA AACATTTAGG GAAGAGGACA TCATTAGTGA TAAAGAGCTT 
GGGCTTCTTG AGCTGCCGGC AGTTGTGTGG AGAATTCGTG TGGTACGGTG GCCTTGTTTG 
CTGCTAAAAA ATGAGCTTCT TCTTGCTCTC AGTCAGGCAG CAGAGCTGGT GGCTGATGAC 
AGGACACATT GGAACAAGAT ATGTAACAAC GAGTACAGGC GATGTGCAGT AATTGAAGCT 
TATGACAGCA TACGTCACTT GCTGCTGGAA ATAATCAAGG AGAGAACTAA TGAACACATT 
ATTGTCAATC AGCTATTCCT TGCATTTGAT GGTGCAATGG AATATGGAAA GTTCACTGAA 
GAATATAGGC TAACCTTATT ACCTCAAATT CATAAGTATG TAATCTCCTT AGTGGAGCAG 
CTTCTATTGA AGGACAAGGA TCAGATCAAG ATAGTTCGTA CCTTGCAGGA TCTGTATGAT 
CTTGCAGTGC ATGATTTTCC AAAAATCAAG AAGGACTTTG AACAGCTGAG GCGAGAGGGG 
CTAGCACTAT CAAGACCAAC CGAATCTCAG CTGCTTTTTC AGGATGCCAT AAAATGCCCA 
GATGACAATG ATGTTAGCTT CTATAAGCAG GTGAGAAGAC TCCATACGAT TCTCACATCC 
AGGGATTCTA TGGACGATGT TCCAAAGAAC CCCGAGGCTC GTCGGCGCAT AACTTTCTTC 
AGCAACTCCC TGTTCATGAA CATGCCTCGT GCTCCCACCG TTCAGAGGAT GATGGCATTC 
AGTGTTTTAA CCCCATATTA CAATGAAGAT GTTCTGTACA ACAAGGACCA GCTTCGTCGT 
GAGAATGAAG ATGGCATCTC AATCTTGTTT TATCTTCAAA AGATTTATGA AGATGATTGG 
AAAAACTTTT TAGAGCGCAT GCAAAGGGAA GGAATGGCTA GTGATGATGG CATTTGGGCT 
GGAAAATTCC AGGATCTCCG ACTTTGGGCC TCGTACAGGG GGCAGACCTT AGCACGGACT 
GTGAGGGGAA TGATGTACTA CTACAGGGCT CTCAAGATGC TTGCTTTTCT TGATAATGCA 
TCTGAAGTAG AAATTACAGA GGGAACAAAA CAGCTGGCTT CTTTTGGTTC AGTTCAGTAT 
GAGAATGATG TATATCCCAT GAACGGGGGT TTATCACAAC GACCTCGAAG GAGGTTAGAG 
AGAGGAACAA GTACTGTAAG TCAATTGTTT AAAGGCCAGG AAGATGGTGC TGCTATCATG 
AAGTACACCT ATGTGGTCGC TTGCCAAATA TATGGAAACC AGAAAAAGGC GAAAGATCAA 
CGTGCTGAAG ATATCCTAAC TCTTATGAAG AAAAATGATG CCCTCCGTGT TGCTTATGTC 
GATGAGGTCC ATCCTGAGAT TGGTGATACG CAATACTATT CTGTCCTTGT TAAATTTGAT 
CCTGTCTTAC AGAGAGAGGT TGAGATATAC CGTATTAGGT TGCCAGGTCA ACTGAAACTT 
GGGGAGGGAA AACCTGAAAA TCAGAACCAT GCCATCATAT TCACACGAGG TGATGCTGTG 
CAAACGATTG ATATGAACCA GGATAATTAT TTTGAGGAGG CCCTCAAGAT GCGTAACCTA 
CTAGAACAGT ACGACTATTA TCATGGAAGC CAGAAACCAA CGCTTTTGGG CGTCCGGGAG 
CATGTTTTTA CTGGATCGGT GTCTTCACTT GCTTGGTTCA TGTCTGCACA AGAAACAAGC 
TTTGTTACCC TTGGACAGCG AGTTCTAGCT AATCCGTTGA AGGTTCGGAT GCATTATGGG 
CATCCTGATG TATTTGATCG CCTTTGGTTT TTAACCCGAG GTGGTATAAG CAAGGCATCC 
AGAGTAATCA ATATCAGCGA GGACATATTT GCAGGTTTCA ACTGTACCCT ACGCGGTGGC 
AATGTTAGCC ACCATGAGTA TATCCAGGTT GGTAAGGGAC GTGATGTTGG GCTCAATCAG 
ATATCGATGT TTGAAGCAAA GGTTTCTAGT GGCAATGGTG AACAGACCCT AAGTAGGGAT 
ATCTACAGAC TTGGTCATAG GCTGGATTTC TTTAGGAGTC TTTCTGTGTT CTATACAACA 
GTAGGATTCT ACTTCAACAC AATGATGGTG GTCTTGACAG TTTATACATT TGTTTGGGGA 
CGCCTCTATC TAGCTCTGAG TGGTCTTGAG GCTGGAATTC AGGGTAGCGC TAATGCTACC 
AACAATAAAG CCTTGGGTGC CGTTCTGAAT CAGCAATTTG TCATACAGCT TGGCCTCTTC 
ACTGCCTTGC CAATGATTAT AGAGAACTCT CTTGAACAGG GTTTTCTGCC TGCTGTCTGG 
GATTTTTTCA CTATGCAAAT GATGTTTTCG TCCGTGTTCT ACACATTTTC CATGGGGACG 
AAAAGCCATT ACTATGGCCG GACAATTCTT CATGGCGGTG CTAAGTATCG TGCTACTGGC 
CGTGGTTTTG TTGTGCAGCA TAAGAGTTTT GCTGAAAATT ACAGGCTGTA TGCTAGGAGC 
CACTTCATAA AGGCAATAGA ACTTGGAATA GTATTGACTG TGTATGCAGC TCACAGTGTG 
ATTGCAAGGG ATACACTTGT TTACATAATC ATGATGATAT CTAGCTGGTT TCTAGTGGTA 
TCATGGATCA TGGCTCCATT TGCTTTTAAT CCATCTGGCT TTGATTGGTT GAAAACAGTG 
TATGACTTTG ATGATTTCAT GAACTGGATC TGGTACCCTG GTAGTATCTT TTCTAAGGCT 
GAACACAGCT GGGAAGTTTG GTGGTTTGAG GAGCAGGATC ATCTACGAAC AACTGGTCTT 
TGGGGAAAGA TTTTAGAAAT ATTGTTAGAT CTCAGATACT TCTTTTTTCA GTATGGGGTT 
GTATACCAGT TGAAGATCGC AAATGAAAGC AGAAGTATTG CTGTTTATCT GCTTTCCTGG 
ATTTGTGTGG CTGTGATTTT CGGCATTTTT GTCCTAATGT CATATGCTCG AGACAAGTAT 
GCTGCAAAAC AACACCTTTA CTACCGGGTC ATCCAATCTG GTGTCATTAT TCTTGCAGTG 
CTAGTGCTGA TAATATTTCT GAAATTTACT AAATTTCAAA TCATTGACAT CTTCACAAGC 
CTTTTGGCAT TCATTCCTAC TGGTTGGGGC TTGATTTCCA TTGCTCAAGT GATTAGACCA 
TTCATTGAGT CTACTGTGGT CTGGGCCAGT GTTGTTTCTG TGGCACGTTT GTATGAGATA 
CTGCTTGGGG TGTTTGTTAT GGCGCCAGTT GCATTCTTTT CCTGGTTGCC TGGATTTCAG 
GAAATGCAGA CGAGGGTACT TTTCAATGAA GCTTTCAGCA GAGGCCTCCA GATATCCCGC 
ATTCTTGCTG GCAAGAAAAC AATTGCAGTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC4325131 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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