LOC4342015 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC4342015
Entrez Gene ID	4342015
Full Name	putative disease resistance protein RGA1
Gene Type	protein-coding
Organism	Oryza sativa Japonica Group(Japanese rice)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015788667.1	XP_015644153.1	putative disease resistance protein RGA1
XM_015788668.1	XP_015644154.1	putative disease resistance protein RGA1
XM_015788666.2	XP_015644152.1	putative disease resistance protein RGA1
XM_015788667.2	XP_015644153.1	putative disease resistance protein RGA1
XM_015788666.1	XP_015644152.1	putative disease resistance protein RGA1
XM_015788668.2	XP_015644154.1	putative disease resistance protein RGA1
XM_015788669.1	XP_015644155.1	putative disease resistance protein RGA1
XM_015788669.2	XP_015644155.1	putative disease resistance protein RGA1

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os06g0594200	NP_001174882.1
Oryza sativa (rice)	Os12g0481700	NP_001176954.1
Oryza sativa (rice)	Os10g0125700	NP_001064077.1
Oryza sativa (rice)	Os01g0781200	NP_001044443.1
Oryza sativa (rice)	Os05g0365300	NP_001055325.1
Oryza sativa (rice)	Os04g0111900	NP_001052034.1
Oryza sativa (rice)	Os03g0566700	NP_001173500.1
Oryza sativa (rice)	Os02g0282000	NP_001046563.1
Oryza sativa (rice)	Os04g0621600	NP_001053913.2
Oryza sativa (rice)	Os04g0621900	NP_001053916.1
Oryza sativa (rice)	Os04g0621300	NP_001053912.1
Oryza sativa (rice)	Os08g0296600	NP_001061476.1
Oryza sativa (rice)	Os07g0521800	NP_001059812.2
Oryza sativa (rice)	Os04g0325300	NP_001052471.1
Oryza sativa (rice)	Os01g0113100	NP_001041817.2
Oryza sativa (rice)	Os02g0262800	NP_001046492.1
Oryza sativa (rice)	Os02g0118900	NP_001045696.2
Oryza sativa (rice)	Os01g0781401	NP_001172591.1
Oryza sativa (rice)	Os11g0555300	NP_001176605.1
Oryza sativa (rice)	Os09g0365000	NP_001175803.1
Oryza sativa (rice)	Os04g0312000	NP_001052442.1
Oryza sativa (rice)	Os02g0119000	NP_001045697.2
Oryza sativa (rice)	Os02g0261600	NP_001046489.2
Oryza sativa (rice)	Os05g0414200	NP_001055549.1
Oryza sativa (rice)	Os12g0481400	NP_001066773.1
Oryza sativa (rice)	Os12g0202900	NP_001066373.2
Oryza sativa (rice)	Os01g0782100	NP_001172592.1
Oryza sativa (rice)	Os06g0619000	NP_001058087.1
Oryza sativa (rice)	Os09g0365300	NP_001063004.1
Oryza sativa (rice)	Os12g0489800	NP_001066797.1
Oryza sativa (rice)	Os02g0281900	NP_001046562.2
Oryza sativa (rice)	Os01g0178900	NP_001042195.1
Oryza sativa (rice)	Os11g0263000	NP_001176470.1
Oryza sativa (rice)	Os02g0261400	NP_001046488.2
Oryza sativa (rice)	Os04g0374175	NP_001173899.1
Oryza sativa (rice)	Os12g0511500	NP_001066870.1
Oryza sativa (rice)	Os06g0304700	NP_001057467.2
Oryza sativa (rice)	Os01g0112866	NP_001172136.1
Oryza sativa (rice)	Os02g0261300	NP_001046487.2
Oryza sativa (rice)	Os11g0485900	NP_001067917.2
Oryza sativa (rice)	Os04g0621500	NP_001174096.1
Oryza sativa (rice)	Os12g0204700	NP_001176835.1
Oryza sativa (rice)	Os11g0668000	NP_001068424.2
Oryza sativa (rice)	Os01g0781100	NP_001044442.2
Oryza sativa (rice)	Os06g0621600	NP_001058093.1
Oryza sativa (rice)	Os04g0375300	NP_001052576.1
Oryza sativa (rice)	Os02g0281200	NP_001046560.1
Oryza sativa (rice)	Os06g0267900	NP_001057351.1
Oryza sativa (rice)	Os06g0707700	NP_001058528.1
Oryza sativa (rice)	Os06g0707400	NP_001058527.1
Oryza sativa (rice)	Os02g0263366	NP_001172887.1
Oryza sativa (rice)	Os02g0263432	NP_001172888.1
Oryza sativa (rice)	Os04g0621750	NP_001174097.1
Oryza sativa (rice)	Os03g0573500	NP_001050525.1
Oryza sativa (rice)	Os05g0414300	NP_001055550.1
Oryza sativa (rice)	Os12g0511400	NP_001066869.1
Oryza sativa (rice)	Os11g0429100	NP_001067785.1
Oryza sativa (rice)	Os08g0296700	NP_001061477.1
Oryza sativa (rice)	Os06g0279900	NP_001057389.1
Oryza sativa (rice)	Os06g0621500	NP_001058092.1
Oryza sativa (rice)	Os02g0282500	NP_001046565.1
Oryza sativa (rice)	Os04g0374800	NP_001052575.1
Oryza sativa (rice)	Os04g0622600	NP_001053918.1
Oryza sativa (rice)	Os04g0626450	NP_001174101.1
Oryza sativa (rice)	Os02g0261800	NP_001046490.2
Oryza sativa (rice)	Os02g0262700	NP_001046491.1
Oryza sativa (rice)	Os07g0521500	NP_001059809.2
Oryza sativa (rice)	Os12g0204600	NP_001066379.1

Gene Ontology

The following LOC4342015 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC4342015 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOa35989	XM_015788669.2 Latest version!	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OOa35989	XM_015788666.2 Latest version!	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OOa35989	XM_015788667.2 Latest version!	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OOa35989	XM_015788668.2 Latest version!	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
View Old Accession Versions >>
OOa35989	XM_015788667.1	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OOa35989	XM_015788666.1	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OOa35989	XM_015788668.1	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OOa35989	XM_015788669.1	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC4342015 ( XM_015788667.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015644153.1 Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOa35989
Clone ID Related Accession (Same CDS sequence)	XM_015788667.1 , XM_015788668.1 , XM_015788666.2 , XM_015788667.2 , XM_015788666.1 , XM_015788668.2 , XM_015788669.1 , XM_015788669.2
Accession Version	XM_015788667.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	putative disease resistance protein RGA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379179.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015644153.1
CDS	688..5172
Translation	MESAAVNAARWVVGKALSPLSGGFVEAWAASTELGPNVGAIKMELLYAQGMLHNTRGRETSNPALQQLLLELRGLAYDADDVLDELDYFRIQDELDGTYEAADEHASGCLLGLVLNAHHTARNIKRRYLSASCSCGGDEEASRHANEEEAVAGSGCMRILACSAQSTIHLVGKHLPCFPFAPGLDNCDDSGALHISTMKTPKLKFDRVDLSTRMKHIVEQLKPVCAKVSTILNLELLESCIAISINAEFSRKPGQTLVHPSNVAMNRPVTTSGIIDPEFYGRKGDRSRIIEDITRGDYCDKDLTVIPIVGPGGIGKTTLTQHIYKEVHNHFDVKVWVCVSLNFNLYRLKEEISKWIPKLKDDEKYVDMDDSIEQRLKSKKLLLVLDDMWKCDNEDEWKKFLAPLRKAMTKGNIILVTTRFPVVAEMVKTFDRPIQLEGLEPEEFWELFQACVFGDDKSIDDHANLLETGEKIVKKLKGSPLAAKTVGRLLRNHLDLDDWMRVLESKEWESQTSENDIMPALKLSYDYLSFHLQQCFTYCALFPEDYKFDSEEMIHLWIGQGILHSQDENKTIEDIGLRYLNDLVNYGFFIKDVNEDGSPYYTMHDLLHELALKVSSYEYLSISNTKVMSMQIPPSIRHLSIVIDDMDVNDRATFENIKKDFGTLSKRLDVEKLHSLMIFGKNNGSFVIPFGNLLSKAKALRVILLSVASYAVENMLHNFSNLVHLRYLRINKGYFPELSLSNIISRFYHLRILDVRQCSGHFGLPRDMNHLVRMRHFLVPNDNLHSEIASVGKLKCLQELRRFEVQRQVEAFALRQLGQLEDLKGSLGIYNLENAQAPEEAELSNKSRLHKLILDCDIKRLTKDSLQEEHILESLKPHSNLRELHINGRGGTTCPSWLGVNLSIKGLQTLRLDGVDWNKFPPLGKLLLINEHGEESLVCTTERSFPNLKRLELVGIQRLAKWVGNDSCFVFSLLKVLIIRDCPELTELPFSHSTCPRSEQEMNLTQLRTLKELEIVNCPKLSSFPPIPWRGSPCRALIEGVGLDFQQLDYSKNKQYELCMLVKGKDEHLDGAFWRRLVFGNLTDLKELDLKKCPPLPLEYLLMLSCLRRLTISDSSNVMSHLESENTVSYQFPIEKLEIFECGCSGKELTLFLSHFPKLSMFISRRCNKIRGLGVLGKQMTTMLASSSSPCGNKLEDARFGLEQQQPRGEDEKVSAEAGLLLLPHQLQELNISEIPELILQIHSLVDGMEGGLRGIGGGLQGLHSLRAFSIWGCPTFLSSYYSTSLSCFPFPSSLQKMYLSDVRGMETLAPLSNLSSLTRLTIWKCMDLSGEGLSSLLDHGQLTDLRIQMTPKFFVRSDPSRLQQLQTDDIARVLAPPICSLISSSLTKLTIGFNHEVERITKEQSEALLLLSSLQDLEFYSCRKLQSLPTGLHRLVGLKILEIDWCPAICSLPKGGLPSSLQELDVCRCENEELKRQCRKLRGTIPIIKDREY

Target ORF information:

RefSeq Version	XM_015788667.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015788667.1

ORF Insert Sequence:

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4342015 ( XM_015788668.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015644154.1 Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOa35989
Clone ID Related Accession (Same CDS sequence)	XM_015788667.1 , XM_015788668.1 , XM_015788666.2 , XM_015788667.2 , XM_015788666.1 , XM_015788668.2 , XM_015788669.1 , XM_015788669.2
Accession Version	XM_015788668.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	putative disease resistance protein RGA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015644154.1
CDS	719..5203
Translation	MESAAVNAARWVVGKALSPLSGGFVEAWAASTELGPNVGAIKMELLYAQGMLHNTRGRETSNPALQQLLLELRGLAYDADDVLDELDYFRIQDELDGTYEAADEHASGCLLGLVLNAHHTARNIKRRYLSASCSCGGDEEASRHANEEEAVAGSGCMRILACSAQSTIHLVGKHLPCFPFAPGLDNCDDSGALHISTMKTPKLKFDRVDLSTRMKHIVEQLKPVCAKVSTILNLELLESCIAISINAEFSRKPGQTLVHPSNVAMNRPVTTSGIIDPEFYGRKGDRSRIIEDITRGDYCDKDLTVIPIVGPGGIGKTTLTQHIYKEVHNHFDVKVWVCVSLNFNLYRLKEEISKWIPKLKDDEKYVDMDDSIEQRLKSKKLLLVLDDMWKCDNEDEWKKFLAPLRKAMTKGNIILVTTRFPVVAEMVKTFDRPIQLEGLEPEEFWELFQACVFGDDKSIDDHANLLETGEKIVKKLKGSPLAAKTVGRLLRNHLDLDDWMRVLESKEWESQTSENDIMPALKLSYDYLSFHLQQCFTYCALFPEDYKFDSEEMIHLWIGQGILHSQDENKTIEDIGLRYLNDLVNYGFFIKDVNEDGSPYYTMHDLLHELALKVSSYEYLSISNTKVMSMQIPPSIRHLSIVIDDMDVNDRATFENIKKDFGTLSKRLDVEKLHSLMIFGKNNGSFVIPFGNLLSKAKALRVILLSVASYAVENMLHNFSNLVHLRYLRINKGYFPELSLSNIISRFYHLRILDVRQCSGHFGLPRDMNHLVRMRHFLVPNDNLHSEIASVGKLKCLQELRRFEVQRQVEAFALRQLGQLEDLKGSLGIYNLENAQAPEEAELSNKSRLHKLILDCDIKRLTKDSLQEEHILESLKPHSNLRELHINGRGGTTCPSWLGVNLSIKGLQTLRLDGVDWNKFPPLGKLLLINEHGEESLVCTTERSFPNLKRLELVGIQRLAKWVGNDSCFVFSLLKVLIIRDCPELTELPFSHSTCPRSEQEMNLTQLRTLKELEIVNCPKLSSFPPIPWRGSPCRALIEGVGLDFQQLDYSKNKQYELCMLVKGKDEHLDGAFWRRLVFGNLTDLKELDLKKCPPLPLEYLLMLSCLRRLTISDSSNVMSHLESENTVSYQFPIEKLEIFECGCSGKELTLFLSHFPKLSMFISRRCNKIRGLGVLGKQMTTMLASSSSPCGNKLEDARFGLEQQQPRGEDEKVSAEAGLLLLPHQLQELNISEIPELILQIHSLVDGMEGGLRGIGGGLQGLHSLRAFSIWGCPTFLSSYYSTSLSCFPFPSSLQKMYLSDVRGMETLAPLSNLSSLTRLTIWKCMDLSGEGLSSLLDHGQLTDLRIQMTPKFFVRSDPSRLQQLQTDDIARVLAPPICSLISSSLTKLTIGFNHEVERITKEQSEALLLLSSLQDLEFYSCRKLQSLPTGLHRLVGLKILEIDWCPAICSLPKGGLPSSLQELDVCRCENEELKRQCRKLRGTIPIIKDREY

Target ORF information:

RefSeq Version	XM_015788668.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015788668.1

ORF Insert Sequence:

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4342015 ( XM_015788666.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015644152.1 Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOa35989
Clone ID Related Accession (Same CDS sequence)	XM_015788667.1 , XM_015788668.1 , XM_015788666.2 , XM_015788667.2 , XM_015788666.1 , XM_015788668.2 , XM_015788669.1 , XM_015788669.2
Accession Version	XM_015788666.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	putative disease resistance protein RGA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015788666.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015644152.1
CDS	739..5223
Translation	MESAAVNAARWVVGKALSPLSGGFVEAWAASTELGPNVGAIKMELLYAQGMLHNTRGRETSNPALQQLLLELRGLAYDADDVLDELDYFRIQDELDGTYEAADEHASGCLLGLVLNAHHTARNIKRRYLSASCSCGGDEEASRHANEEEAVAGSGCMRILACSAQSTIHLVGKHLPCFPFAPGLDNCDDSGALHISTMKTPKLKFDRVDLSTRMKHIVEQLKPVCAKVSTILNLELLESCIAISINAEFSRKPGQTLVHPSNVAMNRPVTTSGIIDPEFYGRKGDRSRIIEDITRGDYCDKDLTVIPIVGPGGIGKTTLTQHIYKEVHNHFDVKVWVCVSLNFNLYRLKEEISKWIPKLKDDEKYVDMDDSIEQRLKSKKLLLVLDDMWKCDNEDEWKKFLAPLRKAMTKGNIILVTTRFPVVAEMVKTFDRPIQLEGLEPEEFWELFQACVFGDDKSIDDHANLLETGEKIVKKLKGSPLAAKTVGRLLRNHLDLDDWMRVLESKEWESQTSENDIMPALKLSYDYLSFHLQQCFTYCALFPEDYKFDSEEMIHLWIGQGILHSQDENKTIEDIGLRYLNDLVNYGFFIKDVNEDGSPYYTMHDLLHELALKVSSYEYLSISNTKVMSMQIPPSIRHLSIVIDDMDVNDRATFENIKKDFGTLSKRLDVEKLHSLMIFGKNNGSFVIPFGNLLSKAKALRVILLSVASYAVENMLHNFSNLVHLRYLRINKGYFPELSLSNIISRFYHLRILDVRQCSGHFGLPRDMNHLVRMRHFLVPNDNLHSEIASVGKLKCLQELRRFEVQRQVEAFALRQLGQLEDLKGSLGIYNLENAQAPEEAELSNKSRLHKLILDCDIKRLTKDSLQEEHILESLKPHSNLRELHINGRGGTTCPSWLGVNLSIKGLQTLRLDGVDWNKFPPLGKLLLINEHGEESLVCTTERSFPNLKRLELVGIQRLAKWVGNDSCFVFSLLKVLIIRDCPELTELPFSHSTCPRSEQEMNLTQLRTLKELEIVNCPKLSSFPPIPWRGSPCRALIEGVGLDFQQLDYSKNKQYELCMLVKGKDEHLDGAFWRRLVFGNLTDLKELDLKKCPPLPLEYLLMLSCLRRLTISDSSNVMSHLESENTVSYQFPIEKLEIFECGCSGKELTLFLSHFPKLSMFISRRCNKIRGLGVLGKQMTTMLASSSSPCGNKLEDARFGLEQQQPRGEDEKVSAEAGLLLLPHQLQELNISEIPELILQIHSLVDGMEGGLRGIGGGLQGLHSLRAFSIWGCPTFLSSYYSTSLSCFPFPSSLQKMYLSDVRGMETLAPLSNLSSLTRLTIWKCMDLSGEGLSSLLDHGQLTDLRIQMTPKFFVRSDPSRLQQLQTDDIARVLAPPICSLISSSLTKLTIGFNHEVERITKEQSEALLLLSSLQDLEFYSCRKLQSLPTGLHRLVGLKILEIDWCPAICSLPKGGLPSSLQELDVCRCENEELKRQCRKLRGTIPIIKDREY

Target ORF information:

RefSeq Version	XM_015788666.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015788666.2

ORF Insert Sequence:

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4342015 ( XM_015788667.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015644153.1 Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X2, mRNA.

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CloneID	OOa35989
Clone ID Related Accession (Same CDS sequence)	XM_015788667.1 , XM_015788668.1 , XM_015788666.2 , XM_015788667.2 , XM_015788666.1 , XM_015788668.2 , XM_015788669.1 , XM_015788669.2
Accession Version	XM_015788667.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	putative disease resistance protein RGA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015788667.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015644153.1
CDS	708..5192
Translation	MESAAVNAARWVVGKALSPLSGGFVEAWAASTELGPNVGAIKMELLYAQGMLHNTRGRETSNPALQQLLLELRGLAYDADDVLDELDYFRIQDELDGTYEAADEHASGCLLGLVLNAHHTARNIKRRYLSASCSCGGDEEASRHANEEEAVAGSGCMRILACSAQSTIHLVGKHLPCFPFAPGLDNCDDSGALHISTMKTPKLKFDRVDLSTRMKHIVEQLKPVCAKVSTILNLELLESCIAISINAEFSRKPGQTLVHPSNVAMNRPVTTSGIIDPEFYGRKGDRSRIIEDITRGDYCDKDLTVIPIVGPGGIGKTTLTQHIYKEVHNHFDVKVWVCVSLNFNLYRLKEEISKWIPKLKDDEKYVDMDDSIEQRLKSKKLLLVLDDMWKCDNEDEWKKFLAPLRKAMTKGNIILVTTRFPVVAEMVKTFDRPIQLEGLEPEEFWELFQACVFGDDKSIDDHANLLETGEKIVKKLKGSPLAAKTVGRLLRNHLDLDDWMRVLESKEWESQTSENDIMPALKLSYDYLSFHLQQCFTYCALFPEDYKFDSEEMIHLWIGQGILHSQDENKTIEDIGLRYLNDLVNYGFFIKDVNEDGSPYYTMHDLLHELALKVSSYEYLSISNTKVMSMQIPPSIRHLSIVIDDMDVNDRATFENIKKDFGTLSKRLDVEKLHSLMIFGKNNGSFVIPFGNLLSKAKALRVILLSVASYAVENMLHNFSNLVHLRYLRINKGYFPELSLSNIISRFYHLRILDVRQCSGHFGLPRDMNHLVRMRHFLVPNDNLHSEIASVGKLKCLQELRRFEVQRQVEAFALRQLGQLEDLKGSLGIYNLENAQAPEEAELSNKSRLHKLILDCDIKRLTKDSLQEEHILESLKPHSNLRELHINGRGGTTCPSWLGVNLSIKGLQTLRLDGVDWNKFPPLGKLLLINEHGEESLVCTTERSFPNLKRLELVGIQRLAKWVGNDSCFVFSLLKVLIIRDCPELTELPFSHSTCPRSEQEMNLTQLRTLKELEIVNCPKLSSFPPIPWRGSPCRALIEGVGLDFQQLDYSKNKQYELCMLVKGKDEHLDGAFWRRLVFGNLTDLKELDLKKCPPLPLEYLLMLSCLRRLTISDSSNVMSHLESENTVSYQFPIEKLEIFECGCSGKELTLFLSHFPKLSMFISRRCNKIRGLGVLGKQMTTMLASSSSPCGNKLEDARFGLEQQQPRGEDEKVSAEAGLLLLPHQLQELNISEIPELILQIHSLVDGMEGGLRGIGGGLQGLHSLRAFSIWGCPTFLSSYYSTSLSCFPFPSSLQKMYLSDVRGMETLAPLSNLSSLTRLTIWKCMDLSGEGLSSLLDHGQLTDLRIQMTPKFFVRSDPSRLQQLQTDDIARVLAPPICSLISSSLTKLTIGFNHEVERITKEQSEALLLLSSLQDLEFYSCRKLQSLPTGLHRLVGLKILEIDWCPAICSLPKGGLPSSLQELDVCRCENEELKRQCRKLRGTIPIIKDREY

Target ORF information:

RefSeq Version	XM_015788667.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015788667.2

ORF Insert Sequence:

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4342015 ( XM_015788666.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015644152.1 Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X1, mRNA.

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CloneID	OOa35989
Clone ID Related Accession (Same CDS sequence)	XM_015788667.1 , XM_015788668.1 , XM_015788666.2 , XM_015788667.2 , XM_015788666.1 , XM_015788668.2 , XM_015788669.1 , XM_015788669.2
Accession Version	XM_015788666.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	putative disease resistance protein RGA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015644152.1
CDS	719..5203
Translation	MESAAVNAARWVVGKALSPLSGGFVEAWAASTELGPNVGAIKMELLYAQGMLHNTRGRETSNPALQQLLLELRGLAYDADDVLDELDYFRIQDELDGTYEAADEHASGCLLGLVLNAHHTARNIKRRYLSASCSCGGDEEASRHANEEEAVAGSGCMRILACSAQSTIHLVGKHLPCFPFAPGLDNCDDSGALHISTMKTPKLKFDRVDLSTRMKHIVEQLKPVCAKVSTILNLELLESCIAISINAEFSRKPGQTLVHPSNVAMNRPVTTSGIIDPEFYGRKGDRSRIIEDITRGDYCDKDLTVIPIVGPGGIGKTTLTQHIYKEVHNHFDVKVWVCVSLNFNLYRLKEEISKWIPKLKDDEKYVDMDDSIEQRLKSKKLLLVLDDMWKCDNEDEWKKFLAPLRKAMTKGNIILVTTRFPVVAEMVKTFDRPIQLEGLEPEEFWELFQACVFGDDKSIDDHANLLETGEKIVKKLKGSPLAAKTVGRLLRNHLDLDDWMRVLESKEWESQTSENDIMPALKLSYDYLSFHLQQCFTYCALFPEDYKFDSEEMIHLWIGQGILHSQDENKTIEDIGLRYLNDLVNYGFFIKDVNEDGSPYYTMHDLLHELALKVSSYEYLSISNTKVMSMQIPPSIRHLSIVIDDMDVNDRATFENIKKDFGTLSKRLDVEKLHSLMIFGKNNGSFVIPFGNLLSKAKALRVILLSVASYAVENMLHNFSNLVHLRYLRINKGYFPELSLSNIISRFYHLRILDVRQCSGHFGLPRDMNHLVRMRHFLVPNDNLHSEIASVGKLKCLQELRRFEVQRQVEAFALRQLGQLEDLKGSLGIYNLENAQAPEEAELSNKSRLHKLILDCDIKRLTKDSLQEEHILESLKPHSNLRELHINGRGGTTCPSWLGVNLSIKGLQTLRLDGVDWNKFPPLGKLLLINEHGEESLVCTTERSFPNLKRLELVGIQRLAKWVGNDSCFVFSLLKVLIIRDCPELTELPFSHSTCPRSEQEMNLTQLRTLKELEIVNCPKLSSFPPIPWRGSPCRALIEGVGLDFQQLDYSKNKQYELCMLVKGKDEHLDGAFWRRLVFGNLTDLKELDLKKCPPLPLEYLLMLSCLRRLTISDSSNVMSHLESENTVSYQFPIEKLEIFECGCSGKELTLFLSHFPKLSMFISRRCNKIRGLGVLGKQMTTMLASSSSPCGNKLEDARFGLEQQQPRGEDEKVSAEAGLLLLPHQLQELNISEIPELILQIHSLVDGMEGGLRGIGGGLQGLHSLRAFSIWGCPTFLSSYYSTSLSCFPFPSSLQKMYLSDVRGMETLAPLSNLSSLTRLTIWKCMDLSGEGLSSLLDHGQLTDLRIQMTPKFFVRSDPSRLQQLQTDDIARVLAPPICSLISSSLTKLTIGFNHEVERITKEQSEALLLLSSLQDLEFYSCRKLQSLPTGLHRLVGLKILEIDWCPAICSLPKGGLPSSLQELDVCRCENEELKRQCRKLRGTIPIIKDREY

Target ORF information:

RefSeq Version	XM_015788666.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015788666.1

ORF Insert Sequence:

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4342015 ( XM_015788668.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015644154.1 Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X3, mRNA.

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CloneID	OOa35989
Clone ID Related Accession (Same CDS sequence)	XM_015788667.1 , XM_015788668.1 , XM_015788666.2 , XM_015788667.2 , XM_015788666.1 , XM_015788668.2 , XM_015788669.1 , XM_015788669.2
Accession Version	XM_015788668.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	putative disease resistance protein RGA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015788668.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015644154.1
CDS	739..5223
Translation	MESAAVNAARWVVGKALSPLSGGFVEAWAASTELGPNVGAIKMELLYAQGMLHNTRGRETSNPALQQLLLELRGLAYDADDVLDELDYFRIQDELDGTYEAADEHASGCLLGLVLNAHHTARNIKRRYLSASCSCGGDEEASRHANEEEAVAGSGCMRILACSAQSTIHLVGKHLPCFPFAPGLDNCDDSGALHISTMKTPKLKFDRVDLSTRMKHIVEQLKPVCAKVSTILNLELLESCIAISINAEFSRKPGQTLVHPSNVAMNRPVTTSGIIDPEFYGRKGDRSRIIEDITRGDYCDKDLTVIPIVGPGGIGKTTLTQHIYKEVHNHFDVKVWVCVSLNFNLYRLKEEISKWIPKLKDDEKYVDMDDSIEQRLKSKKLLLVLDDMWKCDNEDEWKKFLAPLRKAMTKGNIILVTTRFPVVAEMVKTFDRPIQLEGLEPEEFWELFQACVFGDDKSIDDHANLLETGEKIVKKLKGSPLAAKTVGRLLRNHLDLDDWMRVLESKEWESQTSENDIMPALKLSYDYLSFHLQQCFTYCALFPEDYKFDSEEMIHLWIGQGILHSQDENKTIEDIGLRYLNDLVNYGFFIKDVNEDGSPYYTMHDLLHELALKVSSYEYLSISNTKVMSMQIPPSIRHLSIVIDDMDVNDRATFENIKKDFGTLSKRLDVEKLHSLMIFGKNNGSFVIPFGNLLSKAKALRVILLSVASYAVENMLHNFSNLVHLRYLRINKGYFPELSLSNIISRFYHLRILDVRQCSGHFGLPRDMNHLVRMRHFLVPNDNLHSEIASVGKLKCLQELRRFEVQRQVEAFALRQLGQLEDLKGSLGIYNLENAQAPEEAELSNKSRLHKLILDCDIKRLTKDSLQEEHILESLKPHSNLRELHINGRGGTTCPSWLGVNLSIKGLQTLRLDGVDWNKFPPLGKLLLINEHGEESLVCTTERSFPNLKRLELVGIQRLAKWVGNDSCFVFSLLKVLIIRDCPELTELPFSHSTCPRSEQEMNLTQLRTLKELEIVNCPKLSSFPPIPWRGSPCRALIEGVGLDFQQLDYSKNKQYELCMLVKGKDEHLDGAFWRRLVFGNLTDLKELDLKKCPPLPLEYLLMLSCLRRLTISDSSNVMSHLESENTVSYQFPIEKLEIFECGCSGKELTLFLSHFPKLSMFISRRCNKIRGLGVLGKQMTTMLASSSSPCGNKLEDARFGLEQQQPRGEDEKVSAEAGLLLLPHQLQELNISEIPELILQIHSLVDGMEGGLRGIGGGLQGLHSLRAFSIWGCPTFLSSYYSTSLSCFPFPSSLQKMYLSDVRGMETLAPLSNLSSLTRLTIWKCMDLSGEGLSSLLDHGQLTDLRIQMTPKFFVRSDPSRLQQLQTDDIARVLAPPICSLISSSLTKLTIGFNHEVERITKEQSEALLLLSSLQDLEFYSCRKLQSLPTGLHRLVGLKILEIDWCPAICSLPKGGLPSSLQELDVCRCENEELKRQCRKLRGTIPIIKDREY

Target ORF information:

RefSeq Version	XM_015788668.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015788668.2

ORF Insert Sequence:

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4342015 ( XM_015788669.1 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015644155.1 Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X4, mRNA.

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CloneID	OOa35989
Clone ID Related Accession (Same CDS sequence)	XM_015788667.1 , XM_015788668.1 , XM_015788666.2 , XM_015788667.2 , XM_015788666.1 , XM_015788668.2 , XM_015788669.1 , XM_015788669.2
Accession Version	XM_015788669.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-29
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	putative disease resistance protein RGA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015379179.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryza sativa Japonica Group Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015644155.1
CDS	719..5203
Translation	MESAAVNAARWVVGKALSPLSGGFVEAWAASTELGPNVGAIKMELLYAQGMLHNTRGRETSNPALQQLLLELRGLAYDADDVLDELDYFRIQDELDGTYEAADEHASGCLLGLVLNAHHTARNIKRRYLSASCSCGGDEEASRHANEEEAVAGSGCMRILACSAQSTIHLVGKHLPCFPFAPGLDNCDDSGALHISTMKTPKLKFDRVDLSTRMKHIVEQLKPVCAKVSTILNLELLESCIAISINAEFSRKPGQTLVHPSNVAMNRPVTTSGIIDPEFYGRKGDRSRIIEDITRGDYCDKDLTVIPIVGPGGIGKTTLTQHIYKEVHNHFDVKVWVCVSLNFNLYRLKEEISKWIPKLKDDEKYVDMDDSIEQRLKSKKLLLVLDDMWKCDNEDEWKKFLAPLRKAMTKGNIILVTTRFPVVAEMVKTFDRPIQLEGLEPEEFWELFQACVFGDDKSIDDHANLLETGEKIVKKLKGSPLAAKTVGRLLRNHLDLDDWMRVLESKEWESQTSENDIMPALKLSYDYLSFHLQQCFTYCALFPEDYKFDSEEMIHLWIGQGILHSQDENKTIEDIGLRYLNDLVNYGFFIKDVNEDGSPYYTMHDLLHELALKVSSYEYLSISNTKVMSMQIPPSIRHLSIVIDDMDVNDRATFENIKKDFGTLSKRLDVEKLHSLMIFGKNNGSFVIPFGNLLSKAKALRVILLSVASYAVENMLHNFSNLVHLRYLRINKGYFPELSLSNIISRFYHLRILDVRQCSGHFGLPRDMNHLVRMRHFLVPNDNLHSEIASVGKLKCLQELRRFEVQRQVEAFALRQLGQLEDLKGSLGIYNLENAQAPEEAELSNKSRLHKLILDCDIKRLTKDSLQEEHILESLKPHSNLRELHINGRGGTTCPSWLGVNLSIKGLQTLRLDGVDWNKFPPLGKLLLINEHGEESLVCTTERSFPNLKRLELVGIQRLAKWVGNDSCFVFSLLKVLIIRDCPELTELPFSHSTCPRSEQEMNLTQLRTLKELEIVNCPKLSSFPPIPWRGSPCRALIEGVGLDFQQLDYSKNKQYELCMLVKGKDEHLDGAFWRRLVFGNLTDLKELDLKKCPPLPLEYLLMLSCLRRLTISDSSNVMSHLESENTVSYQFPIEKLEIFECGCSGKELTLFLSHFPKLSMFISRRCNKIRGLGVLGKQMTTMLASSSSPCGNKLEDARFGLEQQQPRGEDEKVSAEAGLLLLPHQLQELNISEIPELILQIHSLVDGMEGGLRGIGGGLQGLHSLRAFSIWGCPTFLSSYYSTSLSCFPFPSSLQKMYLSDVRGMETLAPLSNLSSLTRLTIWKCMDLSGEGLSSLLDHGQLTDLRIQMTPKFFVRSDPSRLQQLQTDDIARVLAPPICSLISSSLTKLTIGFNHEVERITKEQSEALLLLSSLQDLEFYSCRKLQSLPTGLHRLVGLKILEIDWCPAICSLPKGGLPSSLQELDVCRCENEELKRQCRKLRGTIPIIKDREY

Target ORF information:

RefSeq Version	XM_015788669.1
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015788669.1

ORF Insert Sequence:

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC4342015 ( XM_015788669.2 ) cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice) -> XP_015644155.1 Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOa35989
Clone ID Related Accession (Same CDS sequence)	XM_015788667.1 , XM_015788668.1 , XM_015788666.2 , XM_015788667.2 , XM_015788666.1 , XM_015788668.2 , XM_015788669.1 , XM_015788669.2
Accession Version	XM_015788669.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-06
Organism	Oryza sativa Japonica Group(Japanese rice)
Product	putative disease resistance protein RGA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_029261.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2018 this sequence version replaced XM_015788669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryza sativa Japonica Group Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015644155.1
CDS	739..5223
Translation	MESAAVNAARWVVGKALSPLSGGFVEAWAASTELGPNVGAIKMELLYAQGMLHNTRGRETSNPALQQLLLELRGLAYDADDVLDELDYFRIQDELDGTYEAADEHASGCLLGLVLNAHHTARNIKRRYLSASCSCGGDEEASRHANEEEAVAGSGCMRILACSAQSTIHLVGKHLPCFPFAPGLDNCDDSGALHISTMKTPKLKFDRVDLSTRMKHIVEQLKPVCAKVSTILNLELLESCIAISINAEFSRKPGQTLVHPSNVAMNRPVTTSGIIDPEFYGRKGDRSRIIEDITRGDYCDKDLTVIPIVGPGGIGKTTLTQHIYKEVHNHFDVKVWVCVSLNFNLYRLKEEISKWIPKLKDDEKYVDMDDSIEQRLKSKKLLLVLDDMWKCDNEDEWKKFLAPLRKAMTKGNIILVTTRFPVVAEMVKTFDRPIQLEGLEPEEFWELFQACVFGDDKSIDDHANLLETGEKIVKKLKGSPLAAKTVGRLLRNHLDLDDWMRVLESKEWESQTSENDIMPALKLSYDYLSFHLQQCFTYCALFPEDYKFDSEEMIHLWIGQGILHSQDENKTIEDIGLRYLNDLVNYGFFIKDVNEDGSPYYTMHDLLHELALKVSSYEYLSISNTKVMSMQIPPSIRHLSIVIDDMDVNDRATFENIKKDFGTLSKRLDVEKLHSLMIFGKNNGSFVIPFGNLLSKAKALRVILLSVASYAVENMLHNFSNLVHLRYLRINKGYFPELSLSNIISRFYHLRILDVRQCSGHFGLPRDMNHLVRMRHFLVPNDNLHSEIASVGKLKCLQELRRFEVQRQVEAFALRQLGQLEDLKGSLGIYNLENAQAPEEAELSNKSRLHKLILDCDIKRLTKDSLQEEHILESLKPHSNLRELHINGRGGTTCPSWLGVNLSIKGLQTLRLDGVDWNKFPPLGKLLLINEHGEESLVCTTERSFPNLKRLELVGIQRLAKWVGNDSCFVFSLLKVLIIRDCPELTELPFSHSTCPRSEQEMNLTQLRTLKELEIVNCPKLSSFPPIPWRGSPCRALIEGVGLDFQQLDYSKNKQYELCMLVKGKDEHLDGAFWRRLVFGNLTDLKELDLKKCPPLPLEYLLMLSCLRRLTISDSSNVMSHLESENTVSYQFPIEKLEIFECGCSGKELTLFLSHFPKLSMFISRRCNKIRGLGVLGKQMTTMLASSSSPCGNKLEDARFGLEQQQPRGEDEKVSAEAGLLLLPHQLQELNISEIPELILQIHSLVDGMEGGLRGIGGGLQGLHSLRAFSIWGCPTFLSSYYSTSLSCFPFPSSLQKMYLSDVRGMETLAPLSNLSSLTRLTIWKCMDLSGEGLSSLLDHGQLTDLRIQMTPKFFVRSDPSRLQQLQTDDIARVLAPPICSLISSSLTKLTIGFNHEVERITKEQSEALLLLSSLQDLEFYSCRKLQSLPTGLHRLVGLKILEIDWCPAICSLPKGGLPSSLQELDVCRCENEELKRQCRKLRGTIPIIKDREY

Target ORF information:

RefSeq Version	XM_015788669.2
Organism	Oryza sativa Japonica Group(Japanese rice)
Definition	Oryza sativa Japonica Group putative disease resistance protein RGA1 (LOC4342015), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015788669.2

ORF Insert Sequence:

ATGGAGTCGG CGGCCGTGAA CGCGGCGCGG TGGGTGGTTG GCAAGGCGCT GAGCCCGCTG 
TCGGGAGGGT TCGTGGAGGC GTGGGCGGCC AGCACGGAGC TCGGGCCAAA CGTCGGCGCC 
ATCAAGATGG AGCTGCTCTA CGCGCAGGGG ATGCTGCACA ACACCCGTGG CAGAGAAACC 
AGCAACCCCG CGCTGCAGCA GCTGCTCCTG GAGCTCCGTG GCCTCGCCTA TGATGCCGAC 
GACGTGCTCG ACGAGCTCGA CTACTTCCGC ATCCAGGACG AGCTCGATGG CACCTACGAG 
GCCGCCGATG AGCATGCCAG CGGCTGTCTC CTTGGCCTTG TGCTCAACGC TCACCACACT 
GCCAGAAACA TCAAGAGAAG GTACCTCTCT GCCTCTTGCT CGTGCGGTGG TGACGAGGAA 
GCGAGTCGCC ACGCCAACGA AGAGGAGGCT GTTGCTGGCA GCGGCTGCAT GCGCATCCTC 
GCTTGCAGTG CTCAGAGCAC CATCCATCTC GTTGGTAAAC ACCTTCCATG CTTCCCTTTT 
GCACCTGGTC TTGACAATTG TGATGACTCC GGTGCCTTGC ACATATCTAC CATGAAAACA 
CCAAAGCTGA AGTTCGATAG GGTGGATCTG TCCACAAGAA TGAAGCACAT CGTCGAACAA 
TTGAAGCCCG TATGTGCCAA AGTCTCTACA ATCCTTAATC TAGAGCTGTT GGAATCATGC 
ATTGCCATCA GTATCAATGC TGAATTTTCA AGGAAACCAG GGCAAACGCT AGTTCATCCA 
AGCAACGTTG CTATGAATCG TCCTGTAACT ACTTCAGGGA TTATAGACCC TGAATTTTAT 
GGGAGGAAGG GTGATAGAAG TAGAATTATT GAGGACATTA CTCGGGGTGA TTACTGTGAC 
AAGGATTTGA CCGTCATTCC AATTGTTGGT CCAGGGGGAA TAGGGAAGAC AACTCTCACT 
CAGCACATAT ACAAGGAAGT ACATAACCAT TTTGATGTTA AGGTTTGGGT ATGCGTCTCT 
CTCAATTTCA ATCTGTATAG ATTGAAAGAA GAGATCTCAA AGTGGATACC TAAACTCAAG 
GATGATGAGA AATATGTCGA CATGGATGAT TCAATTGAGC AAAGATTGAA ATCTAAGAAG 
CTTCTTCTTG TCTTGGATGA TATGTGGAAG TGTGATAATG AGGATGAGTG GAAAAAGTTC 
TTAGCTCCCC TCAGAAAAGC AATGACAAAG GGAAACATAA TTCTAGTCAC CACTCGATTT 
CCAGTGGTTG CAGAAATGGT GAAAACATTT GATCGACCAA TACAATTGGA AGGGCTAGAA 
CCTGAAGAGT TCTGGGAATT ATTCCAAGCA TGTGTTTTTG GTGATGACAA ATCAATAGAT 
GATCATGCTA ACTTACTTGA GACAGGAGAA AAGATAGTGA AAAAACTTAA GGGCTCTCCT 
TTGGCAGCAA AAACGGTGGG TAGATTGCTG AGAAATCACC TTGATTTGGA TGATTGGATG 
AGGGTCCTAG AAAGTAAGGA ATGGGAATCA CAAACTAGTG AAAATGATAT TATGCCAGCT 
TTGAAGCTTA GCTATGACTA TCTATCTTTC CATCTACAAC AATGTTTCAC ATATTGTGCT 
TTGTTCCCTG AAGATTACAA GTTTGATAGT GAGGAGATGA TTCACTTGTG GATAGGGCAA 
GGCATTTTAC ATTCCCAGGA TGAAAACAAA ACAATTGAAG ATATTGGGTT GAGATATTTG 
AATGATTTGG TTAATTATGG ATTTTTCATA AAAGATGTGA ATGAAGATGG GTCACCTTAC 
TACACTATGC ATGACCTACT ACATGAGTTA GCATTGAAGG TTTCATCATA TGAATATCTT 
TCTATAAGTA ATACTAAAGT AATGTCTATG CAAATTCCGC CATCTATACG GCACTTATCT 
ATTGTGATAG ATGACATGGA CGTAAACGAC AGAGCAACTT TTGAAAATAT TAAGAAGGAT 
TTTGGCACAC TAAGTAAGAG GCTTGATGTC GAAAAGCTAC ACTCTCTAAT GATATTTGGA 
AAAAACAATG GAAGCTTTGT CATTCCTTTT GGTAATTTGT TAAGTAAAGC AAAAGCCCTC 
CGTGTCATCT TGTTGTCTGT AGCATCTTAT GCTGTGGAGA ATATGTTGCA CAACTTTTCA 
AATCTTGTTC ATCTTCGTTA CTTAAGGATT AATAAGGGAT ATTTTCCTGA ACTAAGTCTG 
TCCAACATTA TTTCAAGATT TTATCACCTG AGGATCCTTG ATGTGAGACA ATGCAGTGGT 
CACTTTGGTT TACCAAGAGA TATGAATCAC CTTGTAAGGA TGCGGCATTT TCTTGTCCCA 
AATGATAATC TTCACTCTGA AATTGCTAGT GTGGGAAAGC TAAAATGTTT ACAAGAGTTA 
AGGAGATTTG AAGTTCAAAG ACAAGTTGAG GCATTTGCAC TAAGACAGCT GGGGCAGTTG 
GAAGACCTAA AAGGGTCATT GGGCATCTAT AATCTTGAAA ATGCGCAGGC ACCAGAGGAA 
GCAGAACTTT CAAACAAAAG TCGCTTACAC AAGTTGATAT TAGATTGTGA TATTAAACGT 
TTGACAAAGG ATTCTTTACA AGAAGAGCAT ATCCTTGAAA GTCTTAAACC ACATAGCAAT 
CTTCGGGAGC TACACATTAA TGGGCGTGGG GGCACTACTT GCCCTTCATG GTTGGGTGTT 
AACTTGTCCA TCAAAGGCTT GCAAACTCTT CGTCTTGATG GTGTAGACTG GAACAAATTT 
CCTCCTCTAG GGAAGTTGTT GTTGATTAAT GAGCATGGTG AAGAATCCCT GGTTTGTACA 
ACAGAGAGGA GCTTTCCGAA TTTAAAAAGG TTAGAGCTTG TTGGCATACA GAGACTAGCA 
AAATGGGTTG GAAATGATTC TTGCTTTGTG TTCTCTCTGC TAAAGGTGCT CATCATAAGA 
GATTGTCCAG AACTCACGGA GTTGCCATTT TCACATTCCA CATGCCCTCG ATCGGAGCAA 
GAGATGAACT TGACCCAACT CCGTACACTA AAGGAGCTTG AGATTGTTAA TTGTCCAAAG 
CTGTCATCAT TCCCTCCCAT TCCTTGGAGA GGCTCTCCAT GCCGTGCGCT GATTGAAGGA 
GTTGGATTGG ATTTTCAGCA GTTGGATTAC TCAAAAAATA AGCAATATGA ATTATGCATG 
CTAGTCAAGG GAAAGGATGA ACATCTAGAT GGTGCATTCT GGAGGAGATT GGTTTTTGGT 
AATCTAACTG ATCTGAAAGA GTTGGATTTG AAGAAATGCC CTCCTTTGCC GCTGGAATAC 
CTCCTAATGC TATCATGTTT GAGGAGACTC ACTATATCTG ACTCAAGCAA TGTCATGTCA 
CATCTTGAAT CTGAGAACAC TGTCAGCTAC CAGTTTCCAA TCGAGAAATT GGAGATCTTT 
GAGTGTGGTT GTAGCGGGAA GGAATTGACG TTGTTTCTAT CTCATTTCCC CAAACTCTCC 
ATGTTTATTT CAAGGAGATG TAATAAGATA AGAGGGCTTG GTGTGCTGGG GAAGCAGATG 
ACAACAATGT TGGCATCGTC ATCATCACCT TGTGGTAATA AGTTGGAGGA TGCTCGTTTC 
GGACTGGAGC AGCAACAACC AAGAGGAGAG GATGAGAAAG TGTCAGCTGA AGCAGGACTA 
TTGCTCTTGC CTCACCAACT TCAGGAGTTG AACATCAGCG AAATCCCAGA GCTGATCCTG 
CAGATCCATT CTCTTGTAGA TGGCATGGAA GGAGGCTTAA GAGGCATAGG AGGAGGATTA 
CAAGGCCTGC ACTCCCTACG AGCATTCTCT ATTTGGGGTT GCCCCACTTT CCTCTCCTCC 
TACTACTCGA CCTCCTTGTC TTGTTTCCCG TTTCCATCCT CCCTTCAAAA GATGTATCTG 
AGTGATGTGA GAGGCATGGA GACTCTTGCT CCCCTCTCAA ACCTCAGCTC TCTCACCAGA 
TTAACCATAT GGAAATGTAT GGACTTAAGT GGTGAGGGCT TGTCTTCTCT GCTCGACCAT 
GGCCAACTCA CAGACCTTCG CATCCAAATG ACCCCCAAAT TCTTCGTCAG GTCTGACCCC 
TCGCGGCTGC AGCAGCTTCA AACGGATGAC ATTGCAAGGG TCCTTGCACC ACCAATCTGC 
AGCCTCATTT CTTCATCACT CACCAAGCTA ACCATTGGTT TTAATCATGA GGTGGAGCGC 
ATCACCAAGG AGCAGAGTGA GGCCCTTCTG CTCCTCTCCT CCCTCCAGGA CCTCGAGTTT 
TACTCCTGCC GGAAGCTGCA GTCCCTCCCC ACTGGGTTAC ACAGACTCGT CGGCCTCAAG 
ATATTAGAGA TCGACTGGTG TCCAGCCATC TGTTCACTGC CCAAGGGTGG CCTTCCGAGT 
TCATTGCAAG AACTAGATGT CTGTCGTTGT GAGAACGAGG AGCTCAAAAG GCAGTGCCGC 
AAGCTAAGAG GAACAATTCC GATCATCAAA GATAGAGAGT ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC4342015 cDNA ORF clone, Oryza sativa Japonica Group(Japanese rice)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

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