BRAF cDNA ORF clone, Odobenus rosmarus divergens(Pacific walrus)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRAF
Entrez Gene ID	101377465
Full Name	B-Raf proto-oncogene, serine/threonine kinase
Gene Type	protein-coding
Organism	Odobenus rosmarus divergens(Pacific walrus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012567280.1	XP_012422734.1	serine/threonine-protein kinase B-raf

Pathways from BioSystems
Gene Ontology

The following BRAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOd68729	XM_012567280.1 Latest version!	Odobenus rosmarus divergens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRAF ( XM_012567280.1 ) cDNA ORF clone, Odobenus rosmarus divergens(Pacific walrus) -> XP_012422734.1 Odobenus rosmarus divergens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.

Price & Availability↑

CloneID	OOd68729
Clone ID Related Accession (Same CDS sequence)	XM_012567280.1
Accession Version	XM_012567280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Odobenus rosmarus divergens(Pacific walrus)
Product	serine/threonine-protein kinase B-raf
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004451079.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Odobenus rosmarus divergens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTGTTGG ACAAATTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAGCT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC TGTTACATCT TCTTCCTCTT CCAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATGTATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACAGTGGT ACCTGCAAGA 
TGTGGAGTTA CAGTCCGAGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACCGG AGAGGAACTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACCTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGCTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC CGCTCCCCCC TCCGATTCTG CTGGGCACCA AATTCTCACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AATTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CTGTCAATAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGACGGA 
GCCCCTTTGA ACCAGCTGAT GCGCTGCCTT CGGAAATGCC AATCCCGGAC TCCCAGTCCC 
CTCCTACATT CTGTCCCCAG TGAAATAGTG TTTGATTTTG AGCCTGGCCC AGTGTTCAGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCGT TACAGAAATC TCCAGGACCT CAGCGGGAAA GGAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACCCTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATT 
CCTGATGGGC AGATCACAGT GGGTCAGAGA ATTGGATCTG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA TGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TCATGGGCTA TTCAACAAAG CCCCAACTAG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATCGAGA CCAAATTCGA GATGATCAAG 
CTTATAGATA TTGCACGGCA GACTGCGCAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGATTTTG GTCTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGAT CCATTTTGTG GATGGCACCA GAAGTGATCC GAATGCAAGA TAAAAACCCA 
TATAGCTTTC AGTCAGATGT CTATGCATTT GGGATTGTTC TATATGAATT GATGACCGGA 
CAGTTACCTT ATTCAAACAT CAACAACAGG GACCAGATAA TTTTTATGGT GGGACGAGGA 
TATCTTTCTC CAGATCTCAG TAAGGTACGG AGTAACTGTC CAAAAGCCAT GAAGAGATTG 
ATGGCAGAGT GCCTCAAAAA GAAAAGAGAT GAGAGGCCAC TCTTTCCCCA AGAAAGGTCC 
AGAAGAAAGT ATAGACTCTT TAACAGTGGT TACCCCTGGA AGATTCTCGC CTCTATTGAG 
CTGCTGGCCC GCTCATTGCC AAAAATTCAC CGCAGTGCAT CAGAACCCTC CTTGAATCGG 
GCTGGCTTCC AAACAGAGGA TTTTAGTCTA TATGCTTGTG CTTCTCCAAA AACACCCATC 
CAGGCAGGGG GATATGGAGA ATTTGCAGCC TTCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012422734.1
CDS	1..2319
Translation	MIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASTDTVTSSSSSSLSVLPSSLSVFQNPTDVSRSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASIAETALTSGSSPSAPPSDSAGHQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRSDGAPLNQLMRCLRKCQSRTPSPLLHSVPSEIVFDFEPGPVFRGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQERSRRKYRLFNSGYPWKILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGEFAAFK

Target ORF information:

RefSeq Version	XM_012567280.1
Organism	Odobenus rosmarus divergens(Pacific walrus)
Definition	Odobenus rosmarus divergens B-Raf proto-oncogene, serine/threonine kinase (BRAF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012567280.1

ORF Insert Sequence:

ATGATTAAGT TGACACAGGA ACATATAGAA GCCCTGTTGG ACAAATTTGG TGGGGAGCAT 
AATCCACCAT CAATATATCT GGAGGCCTAT GAAGAATACA CCAGCAAGCT AGATGCCCTC 
CAACAGCGAG AACAACAGTT ATTGGAATCC CTGGGGAATG GAACTGATTT TTCTGTTTCT 
AGCTCTGCAT CAACGGACAC TGTTACATCT TCTTCCTCTT CCAGCCTTTC AGTGCTACCT 
TCATCTCTTT CAGTTTTTCA AAATCCCACA GATGTATCAC GGAGCAATCC CAAGTCACCA 
CAAAAACCTA TCGTTAGAGT CTTCCTGCCC AATAAACAGA GGACAGTGGT ACCTGCAAGA 
TGTGGAGTTA CAGTCCGAGA CAGTCTAAAG AAAGCTCTGA TGATGAGAGG TCTAATCCCA 
GAGTGCTGTG CTGTTTACAG AATTCAGGAT GGAGAGAAGA AACCGATTGG CTGGGACACT 
GATATTTCCT GGCTCACCGG AGAGGAACTG CATGTAGAAG TGTTGGAAAA TGTTCCGCTT 
ACCACACACA ACTTTGTACG GAAAACCTTT TTCACCTTAG CATTTTGTGA CTTTTGTCGA 
AAGCTGCTTT TCCAGGGTTT TCGCTGTCAA ACATGTGGTT ATAAATTTCA CCAGCGCTGT 
AGTACAGAGG TTCCACTGAT GTGTGTTAAT TATGACCAAC TTGATTTGCT GTTTGTCTCC 
AAGTTCTTTG AACACCACCC AATACCACAG GAGGAGGCCT CCATAGCAGA GACTGCCCTT 
ACGTCTGGAT CATCCCCTTC CGCTCCCCCC TCCGATTCTG CTGGGCACCA AATTCTCACC 
AGTCCGTCTC CTTCAAAATC CATTCCAATT CCACAGCCTT TCCGACCAGC AGATGAAGAT 
CATCGAAATC AATTTGGACA ACGAGACCGG TCCTCATCAG CTCCAAATGT GCATATAAAC 
ACAATAGAAC CTGTCAATAT TGATGACTTG ATTAGAGACC AAGGGTTTCG TAGTGACGGA 
GCCCCTTTGA ACCAGCTGAT GCGCTGCCTT CGGAAATGCC AATCCCGGAC TCCCAGTCCC 
CTCCTACATT CTGTCCCCAG TGAAATAGTG TTTGATTTTG AGCCTGGCCC AGTGTTCAGA 
GGATCAACCA CAGGTTTGTC TGCCACCCCC CCTGCCTCAT TGCCTGGCTC ACTCACTAAT 
GTAAAAGCGT TACAGAAATC TCCAGGACCT CAGCGGGAAA GGAAATCATC TTCATCCTCA 
GAAGATAGGA ATCGAATGAA AACCCTTGGT AGACGGGATT CAAGTGATGA TTGGGAGATT 
CCTGATGGGC AGATCACAGT GGGTCAGAGA ATTGGATCTG GGTCATTTGG GACAGTCTAC 
AAGGGAAAGT GGCATGGTGA TGTGGCAGTG AAAATGTTGA ATGTGACAGC ACCCACACCT 
CAGCAGTTAC AGGCCTTCAA AAATGAAGTA GGAGTACTCA GGAAAACTCG ACATGTGAAT 
ATCCTACTCT TCATGGGCTA TTCAACAAAG CCCCAACTAG CTATTGTTAC CCAGTGGTGT 
GAGGGCTCCA GCTTATATCA CCATCTCCAC ATCATCGAGA CCAAATTCGA GATGATCAAG 
CTTATAGATA TTGCACGGCA GACTGCGCAG GGCATGGATT ACTTACACGC CAAGTCAATC 
ATCCACAGAG ACCTCAAGAG TAATAATATT TTTCTTCATG AAGACCTCAC AGTAAAAATA 
GGTGATTTTG GTCTAGCCAC AGTGAAATCT CGATGGAGTG GGTCCCATCA GTTTGAACAG 
TTGTCTGGAT CCATTTTGTG GATGGCACCA GAAGTGATCC GAATGCAAGA TAAAAACCCA 
TATAGCTTTC AGTCAGATGT CTATGCATTT GGGATTGTTC TATATGAATT GATGACCGGA 
CAGTTACCTT ATTCAAACAT CAACAACAGG GACCAGATAA TTTTTATGGT GGGACGAGGA 
TATCTTTCTC CAGATCTCAG TAAGGTACGG AGTAACTGTC CAAAAGCCAT GAAGAGATTG 
ATGGCAGAGT GCCTCAAAAA GAAAAGAGAT GAGAGGCCAC TCTTTCCCCA AGAAAGGTCC 
AGAAGAAAGT ATAGACTCTT TAACAGTGGT TACCCCTGGA AGATTCTCGC CTCTATTGAG 
CTGCTGGCCC GCTCATTGCC AAAAATTCAC CGCAGTGCAT CAGAACCCTC CTTGAATCGG 
GCTGGCTTCC AAACAGAGGA TTTTAGTCTA TATGCTTGTG CTTCTCCAAA AACACCCATC 
CAGGCAGGGG GATATGGAGA ATTTGCAGCC TTCAAGTAG