WAPL cDNA ORF clone, Numida meleagris(helmeted guineafowl)

Gene
Clones
gRNAs

Summary

Gene Symbol	WAPL
Entrez Gene ID	110400067
Full Name	WAPL cohesin release factor
Gene Type	protein-coding
Organism	Numida meleagris(helmeted guineafowl)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021399665.1	XP_021255340.1	wings apart-like protein homolog isoform X1
XM_021399666.1	XP_021255341.1	wings apart-like protein homolog isoform X2

Pathways from BioSystems
Gene Ontology

The following WAPL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WAPL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONu25357	XM_021399665.1 Latest version!	Numida meleagris WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ONu25358	XM_021399666.1 Latest version!	Numida meleagris WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

WAPL ( XM_021399665.1 ) cDNA ORF clone, Numida meleagris(helmeted guineafowl) -> XP_021255340.1 Numida meleagris WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONu25357
Clone ID Related Accession (Same CDS sequence)	XM_021399665.1
Accession Version	XM_021399665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-06
Organism	Numida meleagris(helmeted guineafowl)
Product	wings apart-like protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034413.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Numida meleagris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCCC GATTTGGAAA GACCTATAGC CGGAAAGGGG GAAACGGCAG CTCCAAGTTT 
GACGAAGTAT TTTCCAACAA ACGGACCACC CTTAGCACGA AATGGGGAGA AACCACCTTC 
ATGGCCAAAT TGGGACAGAA GAGGCCCAGT GTCAAAACGG ACATTTCCGA AGTTCCTAAA 
AGACCCAGAG TTGAAGATGA GGTAACAGAG GATCCATTTG GGTTTGACAG TGATGAAGAA 
TCTCTTCCTG TGTCTTCAAA GAATGTGGCA CAGGGTAAAA GCTCCTCTCA GCTGGACCCA 
AATGAAGCTG CTCAGTCTGA GGACTTCACT TCTCTACTTG AAGCCAACAG CAAAAGTAAT 
CAGCCAGACA GTGGAGAAAA TACTGCATCC AGCAAACAAA TACCTGCTGA CAGTGCTGCT 
CCTCTCATAA AAGAAAAGAG CACAAGCAAA AATGTGGAAG ACAGAGAATG CAAAGGCAGC 
AGTGCTAAAC TCGCATCTGC AGATAAAATG CAGAATTTGA ATGAAGATGA TGAAAAGAAC 
AGTCATTTCA TCTTTGAGAG TGCTGAAGAC AGTAACAAAA CGACTGCTGA AACCACAGAC 
ACTTCTGGAT ACAAAATTGA AGCCAAGGAC TTGCCTGATT CTTGGGACTC CCACGTTGGA 
AAACCAACAG ACTCTCTATC GGAAGCATCT CAGATGAAGG GGCCTTTAAG AACTCACTTG 
TATGAATGGG ACGATGAACC TGAAGACTCC AGAACTGGAG AGTACATATT AGATTTTGAC 
AGTGAGCATC TCTCAGAGAT GAAGAGCAAG GATGAGGAGG GTGATAAAGA AGATACAGAA 
AATCCAAAGG AAGCCATTTG TGAAGAACTT GTGCAAACTA TTCCTAGGCC AAGCAACTGT 
AGGACATACT GTAGATCCAA CAAAGCAAAA CAACAGGGGG CAACAAACTT TGACAAGCTA 
ATGGATGGTA CTAGCCAGTC TTTATCCAAA GCAAACAATG AGTCAAATAA TGATGGTCTG 
AACCCAGCAA AAAAAGCTTC TTTAAGTAGT GGGACCAGTT ATAGAGGGAC AGTTGGACGG 
ACTAGAGACT ACACTGTTCT ACATCCATCT TGCTTGTCAG TTTGTAATGT TACTATACAG 
GACACCATGG AACGAATTGA CGAATTTACC ACAGCAACCC CTACTGACCT TGGAGAAGCT 
GGACGTTTAA GAAAAAAAGC AGACATTGCT ACTACAAAAA CTACAACCAG GTTTCGACCA 
AGCAATACCA AATCCAAAAA GGATGTCAAA TTGGAGTTTT TTGGGTTTGA TGATCAAGAT 
GAGGGAGAGG GAGATGAAGG TGCCTCTAGA AGTTCTGGGA GTTCCAATTA CAAAATTAAG 
TACTTTGGGT TTGATGACTT GAGTGAAAGC GAAGATGAAG ATGAAGAGTG GCAGAGAACA 
GAAAGGAAAG CTAGCAAAAA ACGAACTAGG ACTGCGCCAT CACCCTCACT ACAGCCAACC 
TCCGACAGCA ATGAAAACTG TTCCCAGGAT AATCAGGTCA ATACTAACAC AGATCTTCTG 
GAGTTTTCAG AGGATGCATC TGGTGTGCCT GAAGGCAATA AAAAATCCAC AAATAAACAA 
GGTGATAAAT CCAAGGAAAA CACCAGGAAG ATTTTTAGTG GGCCAAAGCG GTCTCCCACA 
AAAGCTGTAT ATAATGCTAG ACACTGGAAC CAGCCGGAAT CAGAGGTTCT GCCAGCACCA 
CCGGTTTTGA AAACTCCCAG TGTCCCAGTA AGGCTATCTT CAAAGGAGGC AATTCATAAA 
GATGATGGAG TTTTTAAGGC TCCTGCACCA CCTCCCAAAG TGATTAAAAC TGTGACAATA 
CCTACTCAGC CCTATCAAGA GATAGTAACT GCATTGAAAT GTAGGAAAGA AGACAAAGAA 
TTATACACTG TTGTTCAACA TGTAAAGCAC TTCAATGATG TAGTGGAATT TGGTGAAAAT 
CAAGAGTTCA CAGATGATAT TGAGTACTTG CTAAGTGGAT TGAAGAGCAA TCAGCCCCTA 
AACACACGTT GCCTTAGTGT AATCAGCTTG GCGACCAAGT GCGCCATGCC CAGCTTCCGA 
ATGCATCTGA GAGCACATGG GATGGTAGCC ATGGTTTTCA AAACACTGGA TGATTCCCAG 
CACCATCAGA ATTTGTCCCT TTGTACAGCA GCTCTAATGT ACATCTTGAG TAGGGATCGT 
CTGAACATGG ATTTAGACAG AGCTAGTTTA GATCTGATGA TACGGCTGTT GGAATTGGAA 
CAAGATGCTT CTTCTGCCAA GCTGCTGAAT GAGAAAGACA TGAATAAAAT TAAGGAAAAA 
ATTCGGAGAC TGTGTGAAAC TGTTCACAAT AAACATCTTG ATCTCGAAAA CATCACGACG 
GGGCATTTGG CAATGGAGAC ATTATTGTCT CTCACCTCAA AACGAGCTGG GGACTGGTTT 
AAAGAAGAGC TACGACTTCT AGGTGGTCTT GATCATATTG TAGATAAAGT TAAAGAATGT 
GTGGATCACC TTAGCAGTGA CGAAAATGAA GAGAAATTAG TTGCTTCGTT ATGGGGTGCA 
GAAAGATGTT TACGGGTCTT AGAAAGTGTA ACTGTGCATA ATCCAGAAAA TCAGAGCTAC 
CTGATAGCAT ACAAAGACTC ACAACTCATA GTCTCCTCAG CTAAAGCACT GCAGCATTGT 
GAGGAGTTAA TCCAGAGATA TAATCGTGCT GAAGACAGTA TCTGTTTACC AGACAATAAG 
CCTCTGCCTC ACCAGAATGT AACTAACCAT GTGGGTAAAG CAGTGGAAGA CTGCATGAGG 
GCCATCATTG GTGTCTTGCT CAACCTAACA AATGATAATG AATGGGGTAG TACAAAAACA 
GGTGAACAAG ATGGTCTGAT AGGCACGGCT ATGAATTGTG TGCTTCATGT TCCGAAGTTC 
CTACCTCAAG AACAGAGATT CGATATTCGG GTGCTGGGAT TGGGTCTGTT GATCAATCTA 
GTAGAATATA GTGCTCGGAA CAGGCACTGT CTCGTCAATA TGGAAACATC GTGTTCTTTT 
GACTCATTCT GCACGGGAGA AGGAGACGAT AGCCTAAAGA TAACTGGACA GATTGATGCT 
GTTCAGGCTT TAGTGCAGCT GTTTCTTGAG CGTGAGCGAG CAGCACAGCT GGCTGAGAGC 
CAGACAGATA AACTAATTAA AGAAGCTCCT ACTGCTCAGC ATGATAAGAG TGGAGAATGG 
CAAGAAACAA GTGGGGAGAT ACAGTGGGTC TCCACAGAAA AGTCAGATAG TACTGAAGAG 
AAAGATAAGA AGGAAGAAGA TGATGAAGAA CTTGATCTTA ATAAAGCTCT TCAGCATGCT 
GGGAAGCACA TGGAAGACTG CATTGTGGCC TCGTACACAG CATTGCTTCT AGGCTGTCTC 
TGCCAAGAGA GTCCGATCAA CGTGACTACT GTGAGGGAAT ACTTACCTGA AGGAGATTTC 
TCAATAATGA CTGAAATGCT CAAAAAATTT CTCAGTTTTA TGAATCTTAC TTGTGCTGTT 
GGAACAACAG GCCAGAAATC CATCTCTAGG GTGATTGAAT ACTTGGAACA CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021255340.1
CDS	145..3741
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPSVKTDISEVPKRPRVEDEVTEDPFGFDSDEESLPVSSKNVAQGKSSSQLDPNEAAQSEDFTSLLEANSKSNQPDSGENTASSKQIPADSAAPLIKEKSTSKNVEDRECKGSSAKLASADKMQNLNEDDEKNSHFIFESAEDSNKTTAETTDTSGYKIEAKDLPDSWDSHVGKPTDSLSEASQMKGPLRTHLYEWDDEPEDSRTGEYILDFDSEHLSEMKSKDEEGDKEDTENPKEAICEELVQTIPRPSNCRTYCRSNKAKQQGATNFDKLMDGTSQSLSKANNESNNDGLNPAKKASLSSGTSYRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERIDEFTTATPTDLGEAGRLRKKADIATTKTTTRFRPSNTKSKKDVKLEFFGFDDQDEGEGDEGASRSSGSSNYKIKYFGFDDLSESEDEDEEWQRTERKASKKRTRTAPSPSLQPTSDSNENCSQDNQVNTNTDLLEFSEDASGVPEGNKKSTNKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNQPESEVLPAPPVLKTPSVPVRLSSKEAIHKDDGVFKAPAPPPKVIKTVTIPTQPYQEIVTALKCRKEDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSNQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSSDENEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQRYNRAEDSICLPDNKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTAMNCVLHVPKFLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSFCTGEGDDSLKITGQIDAVQALVQLFLERERAAQLAESQTDKLIKEAPTAQHDKSGEWQETSGEIQWVSTEKSDSTEEKDKKEEDDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_021399665.1
Organism	Numida meleagris(helmeted guineafowl)
Definition	Numida meleagris WAPL cohesin release factor (WAPL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021399665.1

ORF Insert Sequence:

ATGACATCCC GATTTGGAAA GACCTATAGC CGGAAAGGGG GAAACGGCAG CTCCAAGTTT 
GACGAAGTAT TTTCCAACAA ACGGACCACC CTTAGCACGA AATGGGGAGA AACCACCTTC 
ATGGCCAAAT TGGGACAGAA GAGGCCCAGT GTCAAAACGG ACATTTCCGA AGTTCCTAAA 
AGACCCAGAG TTGAAGATGA GGTAACAGAG GATCCATTTG GGTTTGACAG TGATGAAGAA 
TCTCTTCCTG TGTCTTCAAA GAATGTGGCA CAGGGTAAAA GCTCCTCTCA GCTGGACCCA 
AATGAAGCTG CTCAGTCTGA GGACTTCACT TCTCTACTTG AAGCCAACAG CAAAAGTAAT 
CAGCCAGACA GTGGAGAAAA TACTGCATCC AGCAAACAAA TACCTGCTGA CAGTGCTGCT 
CCTCTCATAA AAGAAAAGAG CACAAGCAAA AATGTGGAAG ACAGAGAATG CAAAGGCAGC 
AGTGCTAAAC TCGCATCTGC AGATAAAATG CAGAATTTGA ATGAAGATGA TGAAAAGAAC 
AGTCATTTCA TCTTTGAGAG TGCTGAAGAC AGTAACAAAA CGACTGCTGA AACCACAGAC 
ACTTCTGGAT ACAAAATTGA AGCCAAGGAC TTGCCTGATT CTTGGGACTC CCACGTTGGA 
AAACCAACAG ACTCTCTATC GGAAGCATCT CAGATGAAGG GGCCTTTAAG AACTCACTTG 
TATGAATGGG ACGATGAACC TGAAGACTCC AGAACTGGAG AGTACATATT AGATTTTGAC 
AGTGAGCATC TCTCAGAGAT GAAGAGCAAG GATGAGGAGG GTGATAAAGA AGATACAGAA 
AATCCAAAGG AAGCCATTTG TGAAGAACTT GTGCAAACTA TTCCTAGGCC AAGCAACTGT 
AGGACATACT GTAGATCCAA CAAAGCAAAA CAACAGGGGG CAACAAACTT TGACAAGCTA 
ATGGATGGTA CTAGCCAGTC TTTATCCAAA GCAAACAATG AGTCAAATAA TGATGGTCTG 
AACCCAGCAA AAAAAGCTTC TTTAAGTAGT GGGACCAGTT ATAGAGGGAC AGTTGGACGG 
ACTAGAGACT ACACTGTTCT ACATCCATCT TGCTTGTCAG TTTGTAATGT TACTATACAG 
GACACCATGG AACGAATTGA CGAATTTACC ACAGCAACCC CTACTGACCT TGGAGAAGCT 
GGACGTTTAA GAAAAAAAGC AGACATTGCT ACTACAAAAA CTACAACCAG GTTTCGACCA 
AGCAATACCA AATCCAAAAA GGATGTCAAA TTGGAGTTTT TTGGGTTTGA TGATCAAGAT 
GAGGGAGAGG GAGATGAAGG TGCCTCTAGA AGTTCTGGGA GTTCCAATTA CAAAATTAAG 
TACTTTGGGT TTGATGACTT GAGTGAAAGC GAAGATGAAG ATGAAGAGTG GCAGAGAACA 
GAAAGGAAAG CTAGCAAAAA ACGAACTAGG ACTGCGCCAT CACCCTCACT ACAGCCAACC 
TCCGACAGCA ATGAAAACTG TTCCCAGGAT AATCAGGTCA ATACTAACAC AGATCTTCTG 
GAGTTTTCAG AGGATGCATC TGGTGTGCCT GAAGGCAATA AAAAATCCAC AAATAAACAA 
GGTGATAAAT CCAAGGAAAA CACCAGGAAG ATTTTTAGTG GGCCAAAGCG GTCTCCCACA 
AAAGCTGTAT ATAATGCTAG ACACTGGAAC CAGCCGGAAT CAGAGGTTCT GCCAGCACCA 
CCGGTTTTGA AAACTCCCAG TGTCCCAGTA AGGCTATCTT CAAAGGAGGC AATTCATAAA 
GATGATGGAG TTTTTAAGGC TCCTGCACCA CCTCCCAAAG TGATTAAAAC TGTGACAATA 
CCTACTCAGC CCTATCAAGA GATAGTAACT GCATTGAAAT GTAGGAAAGA AGACAAAGAA 
TTATACACTG TTGTTCAACA TGTAAAGCAC TTCAATGATG TAGTGGAATT TGGTGAAAAT 
CAAGAGTTCA CAGATGATAT TGAGTACTTG CTAAGTGGAT TGAAGAGCAA TCAGCCCCTA 
AACACACGTT GCCTTAGTGT AATCAGCTTG GCGACCAAGT GCGCCATGCC CAGCTTCCGA 
ATGCATCTGA GAGCACATGG GATGGTAGCC ATGGTTTTCA AAACACTGGA TGATTCCCAG 
CACCATCAGA ATTTGTCCCT TTGTACAGCA GCTCTAATGT ACATCTTGAG TAGGGATCGT 
CTGAACATGG ATTTAGACAG AGCTAGTTTA GATCTGATGA TACGGCTGTT GGAATTGGAA 
CAAGATGCTT CTTCTGCCAA GCTGCTGAAT GAGAAAGACA TGAATAAAAT TAAGGAAAAA 
ATTCGGAGAC TGTGTGAAAC TGTTCACAAT AAACATCTTG ATCTCGAAAA CATCACGACG 
GGGCATTTGG CAATGGAGAC ATTATTGTCT CTCACCTCAA AACGAGCTGG GGACTGGTTT 
AAAGAAGAGC TACGACTTCT AGGTGGTCTT GATCATATTG TAGATAAAGT TAAAGAATGT 
GTGGATCACC TTAGCAGTGA CGAAAATGAA GAGAAATTAG TTGCTTCGTT ATGGGGTGCA 
GAAAGATGTT TACGGGTCTT AGAAAGTGTA ACTGTGCATA ATCCAGAAAA TCAGAGCTAC 
CTGATAGCAT ACAAAGACTC ACAACTCATA GTCTCCTCAG CTAAAGCACT GCAGCATTGT 
GAGGAGTTAA TCCAGAGATA TAATCGTGCT GAAGACAGTA TCTGTTTACC AGACAATAAG 
CCTCTGCCTC ACCAGAATGT AACTAACCAT GTGGGTAAAG CAGTGGAAGA CTGCATGAGG 
GCCATCATTG GTGTCTTGCT CAACCTAACA AATGATAATG AATGGGGTAG TACAAAAACA 
GGTGAACAAG ATGGTCTGAT AGGCACGGCT ATGAATTGTG TGCTTCATGT TCCGAAGTTC 
CTACCTCAAG AACAGAGATT CGATATTCGG GTGCTGGGAT TGGGTCTGTT GATCAATCTA 
GTAGAATATA GTGCTCGGAA CAGGCACTGT CTCGTCAATA TGGAAACATC GTGTTCTTTT 
GACTCATTCT GCACGGGAGA AGGAGACGAT AGCCTAAAGA TAACTGGACA GATTGATGCT 
GTTCAGGCTT TAGTGCAGCT GTTTCTTGAG CGTGAGCGAG CAGCACAGCT GGCTGAGAGC 
CAGACAGATA AACTAATTAA AGAAGCTCCT ACTGCTCAGC ATGATAAGAG TGGAGAATGG 
CAAGAAACAA GTGGGGAGAT ACAGTGGGTC TCCACAGAAA AGTCAGATAG TACTGAAGAG 
AAAGATAAGA AGGAAGAAGA TGATGAAGAA CTTGATCTTA ATAAAGCTCT TCAGCATGCT 
GGGAAGCACA TGGAAGACTG CATTGTGGCC TCGTACACAG CATTGCTTCT AGGCTGTCTC 
TGCCAAGAGA GTCCGATCAA CGTGACTACT GTGAGGGAAT ACTTACCTGA AGGAGATTTC 
TCAATAATGA CTGAAATGCT CAAAAAATTT CTCAGTTTTA TGAATCTTAC TTGTGCTGTT 
GGAACAACAG GCCAGAAATC CATCTCTAGG GTGATTGAAT ACTTGGAACA CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WAPL ( XM_021399666.1 ) cDNA ORF clone, Numida meleagris(helmeted guineafowl) -> XP_021255341.1 Numida meleagris WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONu25358
Clone ID Related Accession (Same CDS sequence)	XM_021399666.1
Accession Version	XM_021399666.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-06
Organism	Numida meleagris(helmeted guineafowl)
Product	wings apart-like protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034413.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Numida meleagris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCCC GATTTGGAAA GACCTATAGC CGGAAAGGGG GAAACGGCAG CTCCAAGTTT 
GACGAAGTAT TTTCCAACAA ACGGACCACC CTTAGCACGA AATGGGGAGA AACCACCTTC 
ATGGCCAAAT TGGGACAGAA GAGGCCCAGT GTCAAAACGG ACATTTCCGA AGTTCCTAAA 
AGACCCAGAG TTGAAGATGA GGTAACAGAG GATCCATTTG GGTTTGACAG TGATGAAGAA 
TCTCTTCCTG TGTCTTCAAA GAATGTGGCA CAGGGTAAAA GCTCCTCTCA GCTGGACCCA 
AATGAAGCTG CTCAGTCTGA GGACTTCACT TCTCTACTTG AAGCCAACAG CAAAAGTAAT 
CAGCCAGACA GTGGAGAAAA TACTGCATCC AGCAAACAAA TACCTGCTGA CAGTGCTGCT 
CCTCTCATAA AAGAAAAGAG CACAAGCAAA AATGTGGAAG ACAGAGAATG CAAAGGCAGC 
AGTGCTAAAC TCGCATCTGC AGATAAAATG CAGAATTTGA ATGAAGATGA TGAAAAGAAC 
AGTCATTTCA TCTTTGAGAG TGCTGAAGAC AGTAACAAAA CGACTGCTGA AACCACAGAC 
ACTTCTGGAT ACAAAATTGA AGCCAAGGAC TTGCCTGATT CTTGGGACTC CCACGTTGGA 
AAACCAACAG ACTCTCTATC GGAAGCATCT CAGATGAAGG GGCCTTTAAG AACTCACTTG 
TATGAATGGG ACGATGAACC TGAAGACTCC AGAACTGGAG AGTACATATT AGATTTTGAC 
AGTGAGCATC TCTCAGAGAT GAAGAGCAAG GATGAGGAGG GTGATAAAGA AGATACAGAA 
AATCCAAAGG AAGCCATTTG TGAAGAACTT GTGCAAACTA TTCCTAGGCC AAGCAACTGT 
AGGACATACT GTAGATCCAA CAAAGCAAAA CAACAGGGGG CAACAAACTT TGACAAGCTA 
ATGGATGGTA CTAGCCAGTC TTTATCCAAA GCAAACAATG AGTCAAATAA TGATGGTCTG 
AACCCAGCAA AAAAAGCTTC TTTAAGTAGT GGGACCAGTT ATAGAGGGAC AGTTGGACGG 
ACTAGAGACT ACACTGTTCT ACATCCATCT TGCTTGTCAG TTTGTAATGT TACTATACAG 
GACACCATGG AACGAATTGA CGAATTTACC ACAGCAACCC CTACTGACCT TGGAGAAGCT 
GGACGTTTAA GAAAAAAAGC AGACATTGCT ACTACAAAAA CTACAACCAG GTTTCGACCA 
AGCAATACCA AATCCAAAAA GGATGTCAAA TTGGAGTTTT TTGGGTTTGA TGATCAAGAT 
GAGGGAGAGG GAGATGAAGG TGCCTCTAGA AGTTCTGGGA GTTCCAATTA CAAAATTAAG 
TACTTTGGGT TTGATGACTT GAGTGAAAGC GAAGATGAAG ATGAAGAGTG GCAGAGAACA 
GAAAGGAAAG CTAGCAAAAA ACGAACTAGG ACTGCGCCAT CACCCTCACT ACAGCCAACC 
TCCGACAGCA ATGAAAACTG TTCCCAGGAT AATCAGGTCA ATACTAACAC AGATCTTCTG 
GAGTTTTCAG AGGATGCATC TGGTGTGCCT GAAGGCAATA AAAAATCCAC AAATAAACAA 
GGTGATAAAT CCAAGGAAAA CACCAGGAAG ATTTTTAGTG GGCCAAAGCG GTCTCCCACA 
AAAGCTGTAT ATAATGCTAG ACACTGGAAC CAGCCGGAAT CAGAGGTTCT GCCAGCACCA 
CCGGTTTTGA AAACTCCCAG TGTCCCATTA TACACTGTTG TTCAACATGT AAAGCACTTC 
AATGATGTAG TGGAATTTGG TGAAAATCAA GAGTTCACAG ATGATATTGA GTACTTGCTA 
AGTGGATTGA AGAGCAATCA GCCCCTAAAC ACACGTTGCC TTAGTGTAAT CAGCTTGGCG 
ACCAAGTGCG CCATGCCCAG CTTCCGAATG CATCTGAGAG CACATGGGAT GGTAGCCATG 
GTTTTCAAAA CACTGGATGA TTCCCAGCAC CATCAGAATT TGTCCCTTTG TACAGCAGCT 
CTAATGTACA TCTTGAGTAG GGATCGTCTG AACATGGATT TAGACAGAGC TAGTTTAGAT 
CTGATGATAC GGCTGTTGGA ATTGGAACAA GATGCTTCTT CTGCCAAGCT GCTGAATGAG 
AAAGACATGA ATAAAATTAA GGAAAAAATT CGGAGACTGT GTGAAACTGT TCACAATAAA 
CATCTTGATC TCGAAAACAT CACGACGGGG CATTTGGCAA TGGAGACATT ATTGTCTCTC 
ACCTCAAAAC GAGCTGGGGA CTGGTTTAAA GAAGAGCTAC GACTTCTAGG TGGTCTTGAT 
CATATTGTAG ATAAAGTTAA AGAATGTGTG GATCACCTTA GCAGTGACGA AAATGAAGAG 
AAATTAGTTG CTTCGTTATG GGGTGCAGAA AGATGTTTAC GGGTCTTAGA AAGTGTAACT 
GTGCATAATC CAGAAAATCA GAGCTACCTG ATAGCATACA AAGACTCACA ACTCATAGTC 
TCCTCAGCTA AAGCACTGCA GCATTGTGAG GAGTTAATCC AGAGATATAA TCGTGCTGAA 
GACAGTATCT GTTTACCAGA CAATAAGCCT CTGCCTCACC AGAATGTAAC TAACCATGTG 
GGTAAAGCAG TGGAAGACTG CATGAGGGCC ATCATTGGTG TCTTGCTCAA CCTAACAAAT 
GATAATGAAT GGGGTAGTAC AAAAACAGGT GAACAAGATG GTCTGATAGG CACGGCTATG 
AATTGTGTGC TTCATGTTCC GAAGTTCCTA CCTCAAGAAC AGAGATTCGA TATTCGGGTG 
CTGGGATTGG GTCTGTTGAT CAATCTAGTA GAATATAGTG CTCGGAACAG GCACTGTCTC 
GTCAATATGG AAACATCGTG TTCTTTTGAC TCATTCTGCA CGGGAGAAGG AGACGATAGC 
CTAAAGATAA CTGGACAGAT TGATGCTGTT CAGGCTTTAG TGCAGCTGTT TCTTGAGCGT 
GAGCGAGCAG CACAGCTGGC TGAGAGCCAG ACAGATAAAC TAATTAAAGA AGCTCCTACT 
GCTCAGCATG ATAAGAGTGG AGAATGGCAA GAAACAAGTG GGGAGATACA GTGGGTCTCC 
ACAGAAAAGT CAGATAGTAC TGAAGAGAAA GATAAGAAGG AAGAAGATGA TGAAGAACTT 
GATCTTAATA AAGCTCTTCA GCATGCTGGG AAGCACATGG AAGACTGCAT TGTGGCCTCG 
TACACAGCAT TGCTTCTAGG CTGTCTCTGC CAAGAGAGTC CGATCAACGT GACTACTGTG 
AGGGAATACT TACCTGAAGG AGATTTCTCA ATAATGACTG AAATGCTCAA AAAATTTCTC 
AGTTTTATGA ATCTTACTTG TGCTGTTGGA ACAACAGGCC AGAAATCCAT CTCTAGGGTG 
ATTGAATACT TGGAACACTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021255341.1
CDS	145..3588
Translation	MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPSVKTDISEVPKRPRVEDEVTEDPFGFDSDEESLPVSSKNVAQGKSSSQLDPNEAAQSEDFTSLLEANSKSNQPDSGENTASSKQIPADSAAPLIKEKSTSKNVEDRECKGSSAKLASADKMQNLNEDDEKNSHFIFESAEDSNKTTAETTDTSGYKIEAKDLPDSWDSHVGKPTDSLSEASQMKGPLRTHLYEWDDEPEDSRTGEYILDFDSEHLSEMKSKDEEGDKEDTENPKEAICEELVQTIPRPSNCRTYCRSNKAKQQGATNFDKLMDGTSQSLSKANNESNNDGLNPAKKASLSSGTSYRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERIDEFTTATPTDLGEAGRLRKKADIATTKTTTRFRPSNTKSKKDVKLEFFGFDDQDEGEGDEGASRSSGSSNYKIKYFGFDDLSESEDEDEEWQRTERKASKKRTRTAPSPSLQPTSDSNENCSQDNQVNTNTDLLEFSEDASGVPEGNKKSTNKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNQPESEVLPAPPVLKTPSVPLYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSNQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSSDENEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQRYNRAEDSICLPDNKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTAMNCVLHVPKFLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSFCTGEGDDSLKITGQIDAVQALVQLFLERERAAQLAESQTDKLIKEAPTAQHDKSGEWQETSGEIQWVSTEKSDSTEEKDKKEEDDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Target ORF information:

RefSeq Version	XM_021399666.1
Organism	Numida meleagris(helmeted guineafowl)
Definition	Numida meleagris WAPL cohesin release factor (WAPL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021399666.1

ORF Insert Sequence:

ATGACATCCC GATTTGGAAA GACCTATAGC CGGAAAGGGG GAAACGGCAG CTCCAAGTTT 
GACGAAGTAT TTTCCAACAA ACGGACCACC CTTAGCACGA AATGGGGAGA AACCACCTTC 
ATGGCCAAAT TGGGACAGAA GAGGCCCAGT GTCAAAACGG ACATTTCCGA AGTTCCTAAA 
AGACCCAGAG TTGAAGATGA GGTAACAGAG GATCCATTTG GGTTTGACAG TGATGAAGAA 
TCTCTTCCTG TGTCTTCAAA GAATGTGGCA CAGGGTAAAA GCTCCTCTCA GCTGGACCCA 
AATGAAGCTG CTCAGTCTGA GGACTTCACT TCTCTACTTG AAGCCAACAG CAAAAGTAAT 
CAGCCAGACA GTGGAGAAAA TACTGCATCC AGCAAACAAA TACCTGCTGA CAGTGCTGCT 
CCTCTCATAA AAGAAAAGAG CACAAGCAAA AATGTGGAAG ACAGAGAATG CAAAGGCAGC 
AGTGCTAAAC TCGCATCTGC AGATAAAATG CAGAATTTGA ATGAAGATGA TGAAAAGAAC 
AGTCATTTCA TCTTTGAGAG TGCTGAAGAC AGTAACAAAA CGACTGCTGA AACCACAGAC 
ACTTCTGGAT ACAAAATTGA AGCCAAGGAC TTGCCTGATT CTTGGGACTC CCACGTTGGA 
AAACCAACAG ACTCTCTATC GGAAGCATCT CAGATGAAGG GGCCTTTAAG AACTCACTTG 
TATGAATGGG ACGATGAACC TGAAGACTCC AGAACTGGAG AGTACATATT AGATTTTGAC 
AGTGAGCATC TCTCAGAGAT GAAGAGCAAG GATGAGGAGG GTGATAAAGA AGATACAGAA 
AATCCAAAGG AAGCCATTTG TGAAGAACTT GTGCAAACTA TTCCTAGGCC AAGCAACTGT 
AGGACATACT GTAGATCCAA CAAAGCAAAA CAACAGGGGG CAACAAACTT TGACAAGCTA 
ATGGATGGTA CTAGCCAGTC TTTATCCAAA GCAAACAATG AGTCAAATAA TGATGGTCTG 
AACCCAGCAA AAAAAGCTTC TTTAAGTAGT GGGACCAGTT ATAGAGGGAC AGTTGGACGG 
ACTAGAGACT ACACTGTTCT ACATCCATCT TGCTTGTCAG TTTGTAATGT TACTATACAG 
GACACCATGG AACGAATTGA CGAATTTACC ACAGCAACCC CTACTGACCT TGGAGAAGCT 
GGACGTTTAA GAAAAAAAGC AGACATTGCT ACTACAAAAA CTACAACCAG GTTTCGACCA 
AGCAATACCA AATCCAAAAA GGATGTCAAA TTGGAGTTTT TTGGGTTTGA TGATCAAGAT 
GAGGGAGAGG GAGATGAAGG TGCCTCTAGA AGTTCTGGGA GTTCCAATTA CAAAATTAAG 
TACTTTGGGT TTGATGACTT GAGTGAAAGC GAAGATGAAG ATGAAGAGTG GCAGAGAACA 
GAAAGGAAAG CTAGCAAAAA ACGAACTAGG ACTGCGCCAT CACCCTCACT ACAGCCAACC 
TCCGACAGCA ATGAAAACTG TTCCCAGGAT AATCAGGTCA ATACTAACAC AGATCTTCTG 
GAGTTTTCAG AGGATGCATC TGGTGTGCCT GAAGGCAATA AAAAATCCAC AAATAAACAA 
GGTGATAAAT CCAAGGAAAA CACCAGGAAG ATTTTTAGTG GGCCAAAGCG GTCTCCCACA 
AAAGCTGTAT ATAATGCTAG ACACTGGAAC CAGCCGGAAT CAGAGGTTCT GCCAGCACCA 
CCGGTTTTGA AAACTCCCAG TGTCCCATTA TACACTGTTG TTCAACATGT AAAGCACTTC 
AATGATGTAG TGGAATTTGG TGAAAATCAA GAGTTCACAG ATGATATTGA GTACTTGCTA 
AGTGGATTGA AGAGCAATCA GCCCCTAAAC ACACGTTGCC TTAGTGTAAT CAGCTTGGCG 
ACCAAGTGCG CCATGCCCAG CTTCCGAATG CATCTGAGAG CACATGGGAT GGTAGCCATG 
GTTTTCAAAA CACTGGATGA TTCCCAGCAC CATCAGAATT TGTCCCTTTG TACAGCAGCT 
CTAATGTACA TCTTGAGTAG GGATCGTCTG AACATGGATT TAGACAGAGC TAGTTTAGAT 
CTGATGATAC GGCTGTTGGA ATTGGAACAA GATGCTTCTT CTGCCAAGCT GCTGAATGAG 
AAAGACATGA ATAAAATTAA GGAAAAAATT CGGAGACTGT GTGAAACTGT TCACAATAAA 
CATCTTGATC TCGAAAACAT CACGACGGGG CATTTGGCAA TGGAGACATT ATTGTCTCTC 
ACCTCAAAAC GAGCTGGGGA CTGGTTTAAA GAAGAGCTAC GACTTCTAGG TGGTCTTGAT 
CATATTGTAG ATAAAGTTAA AGAATGTGTG GATCACCTTA GCAGTGACGA AAATGAAGAG 
AAATTAGTTG CTTCGTTATG GGGTGCAGAA AGATGTTTAC GGGTCTTAGA AAGTGTAACT 
GTGCATAATC CAGAAAATCA GAGCTACCTG ATAGCATACA AAGACTCACA ACTCATAGTC 
TCCTCAGCTA AAGCACTGCA GCATTGTGAG GAGTTAATCC AGAGATATAA TCGTGCTGAA 
GACAGTATCT GTTTACCAGA CAATAAGCCT CTGCCTCACC AGAATGTAAC TAACCATGTG 
GGTAAAGCAG TGGAAGACTG CATGAGGGCC ATCATTGGTG TCTTGCTCAA CCTAACAAAT 
GATAATGAAT GGGGTAGTAC AAAAACAGGT GAACAAGATG GTCTGATAGG CACGGCTATG 
AATTGTGTGC TTCATGTTCC GAAGTTCCTA CCTCAAGAAC AGAGATTCGA TATTCGGGTG 
CTGGGATTGG GTCTGTTGAT CAATCTAGTA GAATATAGTG CTCGGAACAG GCACTGTCTC 
GTCAATATGG AAACATCGTG TTCTTTTGAC TCATTCTGCA CGGGAGAAGG AGACGATAGC 
CTAAAGATAA CTGGACAGAT TGATGCTGTT CAGGCTTTAG TGCAGCTGTT TCTTGAGCGT 
GAGCGAGCAG CACAGCTGGC TGAGAGCCAG ACAGATAAAC TAATTAAAGA AGCTCCTACT 
GCTCAGCATG ATAAGAGTGG AGAATGGCAA GAAACAAGTG GGGAGATACA GTGGGTCTCC 
ACAGAAAAGT CAGATAGTAC TGAAGAGAAA GATAAGAAGG AAGAAGATGA TGAAGAACTT 
GATCTTAATA AAGCTCTTCA GCATGCTGGG AAGCACATGG AAGACTGCAT TGTGGCCTCG 
TACACAGCAT TGCTTCTAGG CTGTCTCTGC CAAGAGAGTC CGATCAACGT GACTACTGTG 
AGGGAATACT TACCTGAAGG AGATTTCTCA ATAATGACTG AAATGCTCAA AAAATTTCTC 
AGTTTTATGA ATCTTACTTG TGCTGTTGGA ACAACAGGCC AGAAATCCAT CTCTAGGGTG 
ATTGAATACT TGGAACACTG CTAG