LOC104949544 cDNA ORF clone, Notothenia coriiceps(black rockcod)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104949544
Entrez Gene ID	104949544
Full Name	phosphatidate phosphatase LPIN1-like
Gene Type	protein-coding
Organism	Notothenia coriiceps(black rockcod)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010775914.1	XP_010774216.1	phosphatidate phosphatase LPIN1-like isoform X1
XM_010775915.1	XP_010774217.1	phosphatidate phosphatase LPIN1-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC104949544 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104949544 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo42365	XM_010775914.1 Latest version!	Notothenia coriiceps phosphatidate phosphatase LPIN1-like (LOC104949544), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ONo42366	XM_010775915.1 Latest version!	Notothenia coriiceps phosphatidate phosphatase LPIN1-like (LOC104949544), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104949544 ( XM_010775914.1 ) cDNA ORF clone, Notothenia coriiceps(black rockcod) -> XP_010774216.1 Notothenia coriiceps phosphatidate phosphatase LPIN1-like (LOC104949544), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONo42365
Clone ID Related Accession (Same CDS sequence)	XM_010775914.1
Accession Version	XM_010775914.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-22
Organism	Notothenia coriiceps(black rockcod)
Product	phosphatidate phosphatase LPIN1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011349154.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Notothenia coriiceps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATATCAG CAGAGGATGA AGAAGAGTTT GATGAAACTG CGGAGAACTT TAGCACTGAC 
AACACATGGA GCTGGACCCG TCAGACGATG AACTACGTGG GCCAGCTGGC AGGCCAGGTG 
TTCGTCCAGG TCAAAGAGCT GTACCGAGGC CTGAACCCGG CCACCCTGTC CGGCTGCATC 
GACGTCATCG TAGTGAGGCA GCCCGATGGA TCCCTGCAGT GCTCACCTTT CCATGTCCGC 
TTCGGGAAGA TGGGAGTCCT CCGGTCCCTG GAGAAAGTGG TGGACATGGA GATCAATGGA 
GAGCCAGTGA ATCTACACAT GAAGCTTGGG GACAATGGAG AAGCATTCTT TGTGCAAGAA 
ACAGAAAATG ATCAGGAAGT GGTTCCCTCC TACCTGGCCA CCTCTCCCAT CTTGTCGGAC 
GGGGCCCTCC TGATGAGCTC CTCCCTGCTG GGGAATGGCT CCGGGCCCCC CATACAGACA 
CTGGGCTCCA CGGGGGCCGC TGGGGAGACT GGCAATGGGA TGATGAAGAA GAGGAGGAAG 
AGAAGGAGGA AGGTCCGGGT GGACAGTGTG AGGAGGGAGG ACAGTGGGGA CTACTCCGAG 
GACGAGGACA TGTTTACCAT AGACATCAGT TCCGATGAAA TGACGGGGAT AGGGAGCAAC 
AGGACTTCTC GGGACGTTTT AAGAGATGAG ACGACAGTTG CCCCCGGCAG CTCTTTCAAA 
CAGTCTGGCA TCTACACTCG CTCAGATGGA GACTGGAGCC CCATTCAGAG TCCTGGAAAC 
TCTCGTCCCA TTTCTCCCAA GAGCGACTCT GAGCTATTGA CCAAGCCGTC AGACGCAGCC 
AATCAGGATC CAGCCATGCA TTGGGCGTGG GGAGAGCTCC CGCAGGCTGC CAAGCCGTCT 
TTCCTCCAAG AGAAACCCAA CCATCCGTCA GTTTGCCCGG TAACCATCCC AGTCTCTGAA 
AGCACACACT TCCGCGTGAT AACTCATGAG ATGGAACAGA GTGGACCCTG CGCTGAAATA 
TCAGCACTCA CCCTGATGAT GCCACAGGAG ACGAATGGGG ACACAGTTGG GATGGAGGCC 
ATGACAGCCG GAGGTGTAGC AGCTCAGATG TCTGAAATGG AGGAGACGAT GCCTCTCCCA 
CCGGGAAAAA ACGACTCTCC ATCAAAGAGA AAAGACAAGA GAAGCTGCCA TCTTGGGTCT 
GATGGGATTT ACCTGGATGA CATCACAGAC CTCGAGCCTG AAGTGGCGGC CCTTTATTTC 
CCTAAGAGTG ACGGTGTTGT GGCGGTGCGC AGCGTCTCAG ACCCCGGCCC CCACGGCAGC 
AGCATGTCCC CACAGTCCCT CAGCTCAGGG GGGGACAGCG GAGTGGACAG CTACTGCGAC 
CCTTTGGCGG ACATGCCAAC TATCACCATC TCTCTGTGTG GAGGACTCAA TGACAACAGG 
GAGATCACTA ATGAGCGGTT CCACGAGAAA ATGATCTCTT ACCAGCAGTT TTCGGAAAAC 
CCCTCCATCA TCGACGACCC CAACTTGGTC GTGAAAATTG TCTCCAAATA CTATAATTGG 
AGCTCTGCGG CTCCACTTAT GCTGGCTTTG CAGGCCTTTC AGAAACCGCT GCCAAAGGTT 
GCAGTGGAGA ACATTATGAA GGAGAAGATG CCAAAGAAAG GAGGGAGGTG GTGGTTCTCC 
TGGCGAGGAA GAAACAGCAG CGCCAAATCT GATTCTCTGA ACGTGGGGGC CTGCGGTTAT 
GATGAGCAGG ACGGGAAAAT GTCTAGCAGA CACAAAGAAG ACTCTTCCTC CAGTGATGAA 
GACCACAGGG CAGCCTTACA GAGCTCTGCA CCTGGCCAAT CAGAGCCAGG CCTTACACCA 
GGAGGCGTGT CTTATAAGAA AACACTCAGA CTCACATCAG AGCAGCTGCT GTCCCTCCAG 
CTGCAGGACG GTCCTAACGA TGTTGTGTTC AGCGTGACCA CTCAGTACCA GGGGACGTGT 
CGCTGTCAGG GAACCATCTA CCTCTGGAAC TGGGACGACA AGATCATTAT CTCTGACATA 
GATGGAACCA TCACCAGGTC GGACACACTG GGTCACATCC TGCCCACACT GGGGAAGGAC 
TGGACCCACC AGGGCATTGC ACAGCTCTAC CACAAAGTCA GCCAAAATGG CTACAAGTTC 
CTGTACTGTT CCGCTCGAGC TATCGGCATG GCTGACATGA CCAGAGGTTA CCTCCACTGG 
GTCAACGAGA GAGGCACCAT GCTTCCTATT GGCCCCGTCC TCCTCAGCCC CAGCAGCCTC 
TTCTCAGCTC TGCACAGGGA GGTGATCGAG AAGAAACCCG AGAAATTTAA GGTGGAGTGT 
CTCAACGATA TCAAGAACCT CTTCTACCCC AACACCCAGC CTTTCTATGC AGCGTTTGGC 
AACAGACCAA CGGATGTATA TTCCTATAAA GAAGTCGGGG TGCCACTGAA CAGGATTTTC 
ACCGTTAACC CAAAAGGCGA GCTAGTGCAG GAGCATGCCA AGACCAACAT CTCATCGTAC 
GTGCGTCTGG GTGAGGTGGT GGACCACGTG TTCCCCCTGA AGATGCGATC CTCCTCCTCA 
GACTTCCCCT GCTCGGACAC CTACAGCCAC TTCACCTACT GGAGGCAGCA GCTCCCCCGG 
GTGGAGCCTC AGGGCACTAC GCCTCCACAG AGCCCGCTCC CCAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010774216.1
CDS	129..2819
Translation	MISAEDEEEFDETAENFSTDNTWSWTRQTMNYVGQLAGQVFVQVKELYRGLNPATLSGCIDVIVVRQPDGSLQCSPFHVRFGKMGVLRSLEKVVDMEINGEPVNLHMKLGDNGEAFFVQETENDQEVVPSYLATSPILSDGALLMSSSLLGNGSGPPIQTLGSTGAAGETGNGMMKKRRKRRRKVRVDSVRREDSGDYSEDEDMFTIDISSDEMTGIGSNRTSRDVLRDETTVAPGSSFKQSGIYTRSDGDWSPIQSPGNSRPISPKSDSELLTKPSDAANQDPAMHWAWGELPQAAKPSFLQEKPNHPSVCPVTIPVSESTHFRVITHEMEQSGPCAEISALTLMMPQETNGDTVGMEAMTAGGVAAQMSEMEETMPLPPGKNDSPSKRKDKRSCHLGSDGIYLDDITDLEPEVAALYFPKSDGVVAVRSVSDPGPHGSSMSPQSLSSGGDSGVDSYCDPLADMPTITISLCGGLNDNREITNERFHEKMISYQQFSENPSIIDDPNLVVKIVSKYYNWSSAAPLMLALQAFQKPLPKVAVENIMKEKMPKKGGRWWFSWRGRNSSAKSDSLNVGACGYDEQDGKMSSRHKEDSSSSDEDHRAALQSSAPGQSEPGLTPGGVSYKKTLRLTSEQLLSLQLQDGPNDVVFSVTTQYQGTCRCQGTIYLWNWDDKIIISDIDGTITRSDTLGHILPTLGKDWTHQGIAQLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTMLPIGPVLLSPSSLFSALHREVIEKKPEKFKVECLNDIKNLFYPNTQPFYAAFGNRPTDVYSYKEVGVPLNRIFTVNPKGELVQEHAKTNISSYVRLGEVVDHVFPLKMRSSSSDFPCSDTYSHFTYWRQQLPRVEPQGTTPPQSPLPSS

Target ORF information:

RefSeq Version	XM_010775914.1
Organism	Notothenia coriiceps(black rockcod)
Definition	Notothenia coriiceps phosphatidate phosphatase LPIN1-like (LOC104949544), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010775914.1

ORF Insert Sequence:

ATGATATCAG CAGAGGATGA AGAAGAGTTT GATGAAACTG CGGAGAACTT TAGCACTGAC 
AACACATGGA GCTGGACCCG TCAGACGATG AACTACGTGG GCCAGCTGGC AGGCCAGGTG 
TTCGTCCAGG TCAAAGAGCT GTACCGAGGC CTGAACCCGG CCACCCTGTC CGGCTGCATC 
GACGTCATCG TAGTGAGGCA GCCCGATGGA TCCCTGCAGT GCTCACCTTT CCATGTCCGC 
TTCGGGAAGA TGGGAGTCCT CCGGTCCCTG GAGAAAGTGG TGGACATGGA GATCAATGGA 
GAGCCAGTGA ATCTACACAT GAAGCTTGGG GACAATGGAG AAGCATTCTT TGTGCAAGAA 
ACAGAAAATG ATCAGGAAGT GGTTCCCTCC TACCTGGCCA CCTCTCCCAT CTTGTCGGAC 
GGGGCCCTCC TGATGAGCTC CTCCCTGCTG GGGAATGGCT CCGGGCCCCC CATACAGACA 
CTGGGCTCCA CGGGGGCCGC TGGGGAGACT GGCAATGGGA TGATGAAGAA GAGGAGGAAG 
AGAAGGAGGA AGGTCCGGGT GGACAGTGTG AGGAGGGAGG ACAGTGGGGA CTACTCCGAG 
GACGAGGACA TGTTTACCAT AGACATCAGT TCCGATGAAA TGACGGGGAT AGGGAGCAAC 
AGGACTTCTC GGGACGTTTT AAGAGATGAG ACGACAGTTG CCCCCGGCAG CTCTTTCAAA 
CAGTCTGGCA TCTACACTCG CTCAGATGGA GACTGGAGCC CCATTCAGAG TCCTGGAAAC 
TCTCGTCCCA TTTCTCCCAA GAGCGACTCT GAGCTATTGA CCAAGCCGTC AGACGCAGCC 
AATCAGGATC CAGCCATGCA TTGGGCGTGG GGAGAGCTCC CGCAGGCTGC CAAGCCGTCT 
TTCCTCCAAG AGAAACCCAA CCATCCGTCA GTTTGCCCGG TAACCATCCC AGTCTCTGAA 
AGCACACACT TCCGCGTGAT AACTCATGAG ATGGAACAGA GTGGACCCTG CGCTGAAATA 
TCAGCACTCA CCCTGATGAT GCCACAGGAG ACGAATGGGG ACACAGTTGG GATGGAGGCC 
ATGACAGCCG GAGGTGTAGC AGCTCAGATG TCTGAAATGG AGGAGACGAT GCCTCTCCCA 
CCGGGAAAAA ACGACTCTCC ATCAAAGAGA AAAGACAAGA GAAGCTGCCA TCTTGGGTCT 
GATGGGATTT ACCTGGATGA CATCACAGAC CTCGAGCCTG AAGTGGCGGC CCTTTATTTC 
CCTAAGAGTG ACGGTGTTGT GGCGGTGCGC AGCGTCTCAG ACCCCGGCCC CCACGGCAGC 
AGCATGTCCC CACAGTCCCT CAGCTCAGGG GGGGACAGCG GAGTGGACAG CTACTGCGAC 
CCTTTGGCGG ACATGCCAAC TATCACCATC TCTCTGTGTG GAGGACTCAA TGACAACAGG 
GAGATCACTA ATGAGCGGTT CCACGAGAAA ATGATCTCTT ACCAGCAGTT TTCGGAAAAC 
CCCTCCATCA TCGACGACCC CAACTTGGTC GTGAAAATTG TCTCCAAATA CTATAATTGG 
AGCTCTGCGG CTCCACTTAT GCTGGCTTTG CAGGCCTTTC AGAAACCGCT GCCAAAGGTT 
GCAGTGGAGA ACATTATGAA GGAGAAGATG CCAAAGAAAG GAGGGAGGTG GTGGTTCTCC 
TGGCGAGGAA GAAACAGCAG CGCCAAATCT GATTCTCTGA ACGTGGGGGC CTGCGGTTAT 
GATGAGCAGG ACGGGAAAAT GTCTAGCAGA CACAAAGAAG ACTCTTCCTC CAGTGATGAA 
GACCACAGGG CAGCCTTACA GAGCTCTGCA CCTGGCCAAT CAGAGCCAGG CCTTACACCA 
GGAGGCGTGT CTTATAAGAA AACACTCAGA CTCACATCAG AGCAGCTGCT GTCCCTCCAG 
CTGCAGGACG GTCCTAACGA TGTTGTGTTC AGCGTGACCA CTCAGTACCA GGGGACGTGT 
CGCTGTCAGG GAACCATCTA CCTCTGGAAC TGGGACGACA AGATCATTAT CTCTGACATA 
GATGGAACCA TCACCAGGTC GGACACACTG GGTCACATCC TGCCCACACT GGGGAAGGAC 
TGGACCCACC AGGGCATTGC ACAGCTCTAC CACAAAGTCA GCCAAAATGG CTACAAGTTC 
CTGTACTGTT CCGCTCGAGC TATCGGCATG GCTGACATGA CCAGAGGTTA CCTCCACTGG 
GTCAACGAGA GAGGCACCAT GCTTCCTATT GGCCCCGTCC TCCTCAGCCC CAGCAGCCTC 
TTCTCAGCTC TGCACAGGGA GGTGATCGAG AAGAAACCCG AGAAATTTAA GGTGGAGTGT 
CTCAACGATA TCAAGAACCT CTTCTACCCC AACACCCAGC CTTTCTATGC AGCGTTTGGC 
AACAGACCAA CGGATGTATA TTCCTATAAA GAAGTCGGGG TGCCACTGAA CAGGATTTTC 
ACCGTTAACC CAAAAGGCGA GCTAGTGCAG GAGCATGCCA AGACCAACAT CTCATCGTAC 
GTGCGTCTGG GTGAGGTGGT GGACCACGTG TTCCCCCTGA AGATGCGATC CTCCTCCTCA 
GACTTCCCCT GCTCGGACAC CTACAGCCAC TTCACCTACT GGAGGCAGCA GCTCCCCCGG 
GTGGAGCCTC AGGGCACTAC GCCTCCACAG AGCCCGCTCC CCAGCAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104949544 ( XM_010775915.1 ) cDNA ORF clone, Notothenia coriiceps(black rockcod) -> XP_010774217.1 Notothenia coriiceps phosphatidate phosphatase LPIN1-like (LOC104949544), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONo42366
Clone ID Related Accession (Same CDS sequence)	XM_010775915.1
Accession Version	XM_010775915.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-22
Organism	Notothenia coriiceps(black rockcod)
Product	phosphatidate phosphatase LPIN1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011349154.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Notothenia coriiceps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTACG TGGGCCAGCT GGCAGGCCAG GTGTTCGTCC AGGTCAAAGA GCTGTACCGA 
GGCCTGAACC CGGCCACCCT GTCCGGCTGC ATCGACGTCA TCGTAGTGAG GCAGCCCGAT 
GGATCCCTGC AGTGCTCACC TTTCCATGTC CGCTTCGGGA AGATGGGAGT CCTCCGGTCC 
CTGGAGAAAG TGGTGGACAT GGAGATCAAT GGAGAGCCAG TGAATCTACA CATGAAGCTT 
GGGGACAATG GAGAAGCATT CTTTGTGCAA GAAACAGAAA ATGATCAGGA AGTGGTTCCC 
TCCTACCTGG CCACCTCTCC CATCTTGTCG GACGGGGCCC TCCTGATGAG CTCCTCCCTG 
CTGGGGAATG GCTCCGGGCC CCCCATACAG ACACTGGGCT CCACGGGGGC CGCTGGGGAG 
ACTGGCAATG GGATGATGAA GAAGAGGAGG AAGAGAAGGA GGAAGGTCCG GGTGGACAGT 
GTGAGGAGGG AGGACAGTGG GGACTACTCC GAGGACGAGG ACATGTTTAC CATAGACATC 
AGTTCCGATG AAATGACGGG GATAGGGAGC AACAGGACTT CTCGGGACGT TTTAAGAGAT 
GAGACGACAG TTGCCCCCGG CAGCTCTTTC AAACAGTCTG GCATCTACAC TCGCTCAGAT 
GGAGACTGGA GCCCCATTCA GAGTCCTGGA AACTCTCGTC CCATTTCTCC CAAGAGCGAC 
TCTGAGCTAT TGACCAAGCC GTCAGACGCA GCCAATCAGG ATCCAGCCAT GCATTGGGCG 
TGGGGAGAGC TCCCGCAGGC TGCCAAGCCG TCTTTCCTCC AAGAGAAACC CAACCATCCG 
TCAGTTTGCC CGGTAACCAT CCCAGTCTCT GAAAGCACAC ACTTCCGCGT GATAACTCAT 
GAGATGGAAC AGAGTGGACC CTGCGCTGAA ATATCAGCAC TCACCCTGAT GATGCCACAG 
GAGACGAATG GGGACACAGT TGGGATGGAG GCCATGACAG CCGGAGGTGT AGCAGCTCAG 
ATGTCTGAAA TGGAGGAGAC GATGCCTCTC CCACCGGGAA AAAACGACTC TCCATCAAAG 
AGAAAAGACA AGAGAAGCTG CCATCTTGGG TCTGATGGGA TTTACCTGGA TGACATCACA 
GACCTCGAGC CTGAAGTGGC GGCCCTTTAT TTCCCTAAGA GTGACGGTGT TGTGGCGGTG 
CGCAGCGTCT CAGACCCCGG CCCCCACGGC AGCAGCATGT CCCCACAGTC CCTCAGCTCA 
GGGGGGGACA GCGGAGTGGA CAGCTACTGC GACCCTTTGG CGGACATGCC AACTATCACC 
ATCTCTCTGT GTGGAGGACT CAATGACAAC AGGGAGATCA CTAATGAGCG GTTCCACGAG 
AAAATGATCT CTTACCAGCA GTTTTCGGAA AACCCCTCCA TCATCGACGA CCCCAACTTG 
GTCGTGAAAA TTGTCTCCAA ATACTATAAT TGGAGCTCTG CGGCTCCACT TATGCTGGCT 
TTGCAGGCCT TTCAGAAACC GCTGCCAAAG GTTGCAGTGG AGAACATTAT GAAGGAGAAG 
ATGCCAAAGA AAGGAGGGAG GTGGTGGTTC TCCTGGCGAG GAAGAAACAG CAGCGCCAAA 
TCTGATTCTC TGAACGTGGG GGCCTGCGGT TATGATGAGC AGGACGGGAA AATGTCTAGC 
AGACACAAAG AAGACTCTTC CTCCAGTGAT GAAGACCACA GGGCAGCCTT ACAGAGCTCT 
GCACCTGGCC AATCAGAGCC AGGCCTTACA CCAGGAGGCG TGTCTTATAA GAAAACACTC 
AGACTCACAT CAGAGCAGCT GCTGTCCCTC CAGCTGCAGG ACGGTCCTAA CGATGTTGTG 
TTCAGCGTGA CCACTCAGTA CCAGGGGACG TGTCGCTGTC AGGGAACCAT CTACCTCTGG 
AACTGGGACG ACAAGATCAT TATCTCTGAC ATAGATGGAA CCATCACCAG GTCGGACACA 
CTGGGTCACA TCCTGCCCAC ACTGGGGAAG GACTGGACCC ACCAGGGCAT TGCACAGCTC 
TACCACAAAG TCAGCCAAAA TGGCTACAAG TTCCTGTACT GTTCCGCTCG AGCTATCGGC 
ATGGCTGACA TGACCAGAGG TTACCTCCAC TGGGTCAACG AGAGAGGCAC CATGCTTCCT 
ATTGGCCCCG TCCTCCTCAG CCCCAGCAGC CTCTTCTCAG CTCTGCACAG GGAGGTGATC 
GAGAAGAAAC CCGAGAAATT TAAGGTGGAG TGTCTCAACG ATATCAAGAA CCTCTTCTAC 
CCCAACACCC AGCCTTTCTA TGCAGCGTTT GGCAACAGAC CAACGGATGT ATATTCCTAT 
AAAGAAGTCG GGGTGCCACT GAACAGGATT TTCACCGTTA ACCCAAAAGG CGAGCTAGTG 
CAGGAGCATG CCAAGACCAA CATCTCATCG TACGTGCGTC TGGGTGAGGT GGTGGACCAC 
GTGTTCCCCC TGAAGATGCG ATCCTCCTCC TCAGACTTCC CCTGCTCGGA CACCTACAGC 
CACTTCACCT ACTGGAGGCA GCAGCTCCCC CGGGTGGAGC CTCAGGGCAC TACGCCTCCA 
CAGAGCCCGC TCCCCAGCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010774217.1
CDS	261..2864
Translation	MNYVGQLAGQVFVQVKELYRGLNPATLSGCIDVIVVRQPDGSLQCSPFHVRFGKMGVLRSLEKVVDMEINGEPVNLHMKLGDNGEAFFVQETENDQEVVPSYLATSPILSDGALLMSSSLLGNGSGPPIQTLGSTGAAGETGNGMMKKRRKRRRKVRVDSVRREDSGDYSEDEDMFTIDISSDEMTGIGSNRTSRDVLRDETTVAPGSSFKQSGIYTRSDGDWSPIQSPGNSRPISPKSDSELLTKPSDAANQDPAMHWAWGELPQAAKPSFLQEKPNHPSVCPVTIPVSESTHFRVITHEMEQSGPCAEISALTLMMPQETNGDTVGMEAMTAGGVAAQMSEMEETMPLPPGKNDSPSKRKDKRSCHLGSDGIYLDDITDLEPEVAALYFPKSDGVVAVRSVSDPGPHGSSMSPQSLSSGGDSGVDSYCDPLADMPTITISLCGGLNDNREITNERFHEKMISYQQFSENPSIIDDPNLVVKIVSKYYNWSSAAPLMLALQAFQKPLPKVAVENIMKEKMPKKGGRWWFSWRGRNSSAKSDSLNVGACGYDEQDGKMSSRHKEDSSSSDEDHRAALQSSAPGQSEPGLTPGGVSYKKTLRLTSEQLLSLQLQDGPNDVVFSVTTQYQGTCRCQGTIYLWNWDDKIIISDIDGTITRSDTLGHILPTLGKDWTHQGIAQLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTMLPIGPVLLSPSSLFSALHREVIEKKPEKFKVECLNDIKNLFYPNTQPFYAAFGNRPTDVYSYKEVGVPLNRIFTVNPKGELVQEHAKTNISSYVRLGEVVDHVFPLKMRSSSSDFPCSDTYSHFTYWRQQLPRVEPQGTTPPQSPLPSS

Target ORF information:

RefSeq Version	XM_010775915.1
Organism	Notothenia coriiceps(black rockcod)
Definition	Notothenia coriiceps phosphatidate phosphatase LPIN1-like (LOC104949544), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010775915.1

ORF Insert Sequence:

ATGAACTACG TGGGCCAGCT GGCAGGCCAG GTGTTCGTCC AGGTCAAAGA GCTGTACCGA 
GGCCTGAACC CGGCCACCCT GTCCGGCTGC ATCGACGTCA TCGTAGTGAG GCAGCCCGAT 
GGATCCCTGC AGTGCTCACC TTTCCATGTC CGCTTCGGGA AGATGGGAGT CCTCCGGTCC 
CTGGAGAAAG TGGTGGACAT GGAGATCAAT GGAGAGCCAG TGAATCTACA CATGAAGCTT 
GGGGACAATG GAGAAGCATT CTTTGTGCAA GAAACAGAAA ATGATCAGGA AGTGGTTCCC 
TCCTACCTGG CCACCTCTCC CATCTTGTCG GACGGGGCCC TCCTGATGAG CTCCTCCCTG 
CTGGGGAATG GCTCCGGGCC CCCCATACAG ACACTGGGCT CCACGGGGGC CGCTGGGGAG 
ACTGGCAATG GGATGATGAA GAAGAGGAGG AAGAGAAGGA GGAAGGTCCG GGTGGACAGT 
GTGAGGAGGG AGGACAGTGG GGACTACTCC GAGGACGAGG ACATGTTTAC CATAGACATC 
AGTTCCGATG AAATGACGGG GATAGGGAGC AACAGGACTT CTCGGGACGT TTTAAGAGAT 
GAGACGACAG TTGCCCCCGG CAGCTCTTTC AAACAGTCTG GCATCTACAC TCGCTCAGAT 
GGAGACTGGA GCCCCATTCA GAGTCCTGGA AACTCTCGTC CCATTTCTCC CAAGAGCGAC 
TCTGAGCTAT TGACCAAGCC GTCAGACGCA GCCAATCAGG ATCCAGCCAT GCATTGGGCG 
TGGGGAGAGC TCCCGCAGGC TGCCAAGCCG TCTTTCCTCC AAGAGAAACC CAACCATCCG 
TCAGTTTGCC CGGTAACCAT CCCAGTCTCT GAAAGCACAC ACTTCCGCGT GATAACTCAT 
GAGATGGAAC AGAGTGGACC CTGCGCTGAA ATATCAGCAC TCACCCTGAT GATGCCACAG 
GAGACGAATG GGGACACAGT TGGGATGGAG GCCATGACAG CCGGAGGTGT AGCAGCTCAG 
ATGTCTGAAA TGGAGGAGAC GATGCCTCTC CCACCGGGAA AAAACGACTC TCCATCAAAG 
AGAAAAGACA AGAGAAGCTG CCATCTTGGG TCTGATGGGA TTTACCTGGA TGACATCACA 
GACCTCGAGC CTGAAGTGGC GGCCCTTTAT TTCCCTAAGA GTGACGGTGT TGTGGCGGTG 
CGCAGCGTCT CAGACCCCGG CCCCCACGGC AGCAGCATGT CCCCACAGTC CCTCAGCTCA 
GGGGGGGACA GCGGAGTGGA CAGCTACTGC GACCCTTTGG CGGACATGCC AACTATCACC 
ATCTCTCTGT GTGGAGGACT CAATGACAAC AGGGAGATCA CTAATGAGCG GTTCCACGAG 
AAAATGATCT CTTACCAGCA GTTTTCGGAA AACCCCTCCA TCATCGACGA CCCCAACTTG 
GTCGTGAAAA TTGTCTCCAA ATACTATAAT TGGAGCTCTG CGGCTCCACT TATGCTGGCT 
TTGCAGGCCT TTCAGAAACC GCTGCCAAAG GTTGCAGTGG AGAACATTAT GAAGGAGAAG 
ATGCCAAAGA AAGGAGGGAG GTGGTGGTTC TCCTGGCGAG GAAGAAACAG CAGCGCCAAA 
TCTGATTCTC TGAACGTGGG GGCCTGCGGT TATGATGAGC AGGACGGGAA AATGTCTAGC 
AGACACAAAG AAGACTCTTC CTCCAGTGAT GAAGACCACA GGGCAGCCTT ACAGAGCTCT 
GCACCTGGCC AATCAGAGCC AGGCCTTACA CCAGGAGGCG TGTCTTATAA GAAAACACTC 
AGACTCACAT CAGAGCAGCT GCTGTCCCTC CAGCTGCAGG ACGGTCCTAA CGATGTTGTG 
TTCAGCGTGA CCACTCAGTA CCAGGGGACG TGTCGCTGTC AGGGAACCAT CTACCTCTGG 
AACTGGGACG ACAAGATCAT TATCTCTGAC ATAGATGGAA CCATCACCAG GTCGGACACA 
CTGGGTCACA TCCTGCCCAC ACTGGGGAAG GACTGGACCC ACCAGGGCAT TGCACAGCTC 
TACCACAAAG TCAGCCAAAA TGGCTACAAG TTCCTGTACT GTTCCGCTCG AGCTATCGGC 
ATGGCTGACA TGACCAGAGG TTACCTCCAC TGGGTCAACG AGAGAGGCAC CATGCTTCCT 
ATTGGCCCCG TCCTCCTCAG CCCCAGCAGC CTCTTCTCAG CTCTGCACAG GGAGGTGATC 
GAGAAGAAAC CCGAGAAATT TAAGGTGGAG TGTCTCAACG ATATCAAGAA CCTCTTCTAC 
CCCAACACCC AGCCTTTCTA TGCAGCGTTT GGCAACAGAC CAACGGATGT ATATTCCTAT 
AAAGAAGTCG GGGTGCCACT GAACAGGATT TTCACCGTTA ACCCAAAAGG CGAGCTAGTG 
CAGGAGCATG CCAAGACCAA CATCTCATCG TACGTGCGTC TGGGTGAGGT GGTGGACCAC 
GTGTTCCCCC TGAAGATGCG ATCCTCCTCC TCAGACTTCC CCTGCTCGGA CACCTACAGC 
CACTTCACCT ACTGGAGGCA GCAGCTCCCC CGGGTGGAGC CTCAGGGCAC TACGCCTCCA 
CAGAGCCCGC TCCCCAGCAG CTGA