LOC104947404 cDNA ORF clone, Notothenia coriiceps(black rockcod)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104947404
Entrez Gene ID	104947404
Full Name	tubulin beta-1 chain-like
Gene Type	protein-coding
Organism	Notothenia coriiceps(black rockcod)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010773401.1	XP_010771703.1	tubulin beta-1 chain-like

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Cold adaptation of microtubule assembly and dynamics. Structural interpretation of primary sequence changes present in the alpha- and beta-tubulins of Antarctic fishes.
Detrich HW 3rd, Parker SK, Williams RC Jr, Nogales E, Downing KH
The Journal of biological chemistry275(47)37038-47(2000 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC104947404 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104947404 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo40735	XM_010773401.1 Latest version!	Notothenia coriiceps tubulin beta-1 chain-like (LOC104947404), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104947404 ( XM_010773401.1 ) cDNA ORF clone, Notothenia coriiceps(black rockcod) -> XP_010771703.1 Notothenia coriiceps tubulin beta-1 chain-like (LOC104947404), mRNA.

Price & Availability↑

CloneID	ONo40735
Clone ID Related Accession (Same CDS sequence)	XM_010773401.1
Accession Version	XM_010773401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-22
Organism	Notothenia coriiceps(black rockcod)
Product	tubulin beta-1 chain-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011345269.1) and transcript sequence (AF255553.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Notothenia coriiceps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1284 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAGGGAAA TCGTGCATCT GCAGGCAGGC CAATGTGGAA ATCAAATTGG AGCTAAGTTT 
TGGGAAGTCA TAAGCGACGA ACATGGCATC GACCCGACCG GCTCATACCA GGGGGACAGC 
GACCTGCAGC TGGATCGCAT CAACGTCTAT TACAATGAGG CTTCAGGTGG GAAGTATGTC 
CCCAGGGCCG TGCTGGTGGA CTTGGAGCCC GGTACGATGG ACTCGGTGAG GTCCGGTCCC 
TTCGGCCAGA TCTTTAGACC AGACAACTTT GTCTTTGGCC AGAGTGGAGC TGGTAATAAC 
TGGGCAAAGG GTCACTACAC TGAGGGAGCC GAGCTGGTGG ACTCGGTCCT GGATGTGGTG 
AGGAAGGAGG CGGAGAGCTG CGACTGCCTG CAGGGCTTCC AGCTCACACA CTCCCTGGGT 
GGAGGAACCG GCTCGGGCAT GGGCACGCTG CTCATCAGCA AAATCCGAGA GGAGTATCCA 
GACCGCATCA TGAACACTTT CAGCGTGGTG CCTTCCCCCA AGGTTTCAGA CACAGTTGTG 
GAGCCATACA ACGCCACCCT CTCCGTCCAC CAGCTGGTGG AGAACACAGA TGAGACCTTC 
TGCATTGATA ATGAGGCGCT GTATGACATC TGCTTCCGCA CACTGAAGCT CACCACCCCC 
ACCTACGGAG ATCTCAACCA CCTCGTCTCA GCCACCATGA GCGGTGTCAC CACCTGTCTG 
CGCTTCCCCG GCCAGCTCAA TGCTGATTTG AGGAAACTGG CCGTCAACAT GGTGCCCTTC 
CCCAGGCTGC ACTTCTTCAT GCCCGGCTTC GCCCCATTGA CCAGCCGTGG CAGCCAGCAG 
TACAGGGCTC TGACGGTTCC TGAGCTCACC CAGCAGATGT TCGACTCCAA GAACATGATG 
GCTGCCTGTG ACCCACGCCA CGGCCGCTAC CTCACGGTTG CCGCCATCTT CAGAGGCCGC 
ATGTCCATGA AGGAAGTAGA CGAGCAGATG TTGAATGTGC AGAACAAAAA CAGCAGCTAC 
TTCGTAGAAT GGATCCCAAA CAACGTGAAG ACTGCCGTCT GCGACATCCC TCCCCGTGGC 
CTCAAGATGG CTGCCACCTT CATTGGCAAC AGCACGGCCA TTCAGGAGCT GTTCAAGCGC 
ATCTCAGAGC AGTTCACCGC CATGTTCCGC CGCAAGGCCT TCCTCCACTG GTACACCGGC 
GAGGGCATGG ATGAGATGGA GTTCACAGAG GCTGAGAGCA ACATGAACGA CCTGGTGTCT 
GAGTACCAGC AGTACCAGGA CGCCACCGCT GAGGAGGAGG GCGAGTTTGA GGATGAAGGC 
GAAGAGGACC TGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010771703.1
CDS	99..1436
Translation	MREIVHLQAGQCGNQIGAKFWEVISDEHGIDPTGSYQGDSDLQLDRINVYYNEASGGKYVPRAVLVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETFCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPRHGRYLTVAAIFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEFEDEGEEDLA

Target ORF information:

RefSeq Version	XM_010773401.1
Organism	Notothenia coriiceps(black rockcod)
Definition	Notothenia coriiceps tubulin beta-1 chain-like (LOC104947404), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010773401.1

ORF Insert Sequence:

ATGAGGGAAA TCGTGCATCT GCAGGCAGGC CAATGTGGAA ATCAAATTGG AGCTAAGTTT 
TGGGAAGTCA TAAGCGACGA ACATGGCATC GACCCGACCG GCTCATACCA GGGGGACAGC 
GACCTGCAGC TGGATCGCAT CAACGTCTAT TACAATGAGG CTTCAGGTGG GAAGTATGTC 
CCCAGGGCCG TGCTGGTGGA CTTGGAGCCC GGTACGATGG ACTCGGTGAG GTCCGGTCCC 
TTCGGCCAGA TCTTTAGACC AGACAACTTT GTCTTTGGCC AGAGTGGAGC TGGTAATAAC 
TGGGCAAAGG GTCACTACAC TGAGGGAGCC GAGCTGGTGG ACTCGGTCCT GGATGTGGTG 
AGGAAGGAGG CGGAGAGCTG CGACTGCCTG CAGGGCTTCC AGCTCACACA CTCCCTGGGT 
GGAGGAACCG GCTCGGGCAT GGGCACGCTG CTCATCAGCA AAATCCGAGA GGAGTATCCA 
GACCGCATCA TGAACACTTT CAGCGTGGTG CCTTCCCCCA AGGTTTCAGA CACAGTTGTG 
GAGCCATACA ACGCCACCCT CTCCGTCCAC CAGCTGGTGG AGAACACAGA TGAGACCTTC 
TGCATTGATA ATGAGGCGCT GTATGACATC TGCTTCCGCA CACTGAAGCT CACCACCCCC 
ACCTACGGAG ATCTCAACCA CCTCGTCTCA GCCACCATGA GCGGTGTCAC CACCTGTCTG 
CGCTTCCCCG GCCAGCTCAA TGCTGATTTG AGGAAACTGG CCGTCAACAT GGTGCCCTTC 
CCCAGGCTGC ACTTCTTCAT GCCCGGCTTC GCCCCATTGA CCAGCCGTGG CAGCCAGCAG 
TACAGGGCTC TGACGGTTCC TGAGCTCACC CAGCAGATGT TCGACTCCAA GAACATGATG 
GCTGCCTGTG ACCCACGCCA CGGCCGCTAC CTCACGGTTG CCGCCATCTT CAGAGGCCGC 
ATGTCCATGA AGGAAGTAGA CGAGCAGATG TTGAATGTGC AGAACAAAAA CAGCAGCTAC 
TTCGTAGAAT GGATCCCAAA CAACGTGAAG ACTGCCGTCT GCGACATCCC TCCCCGTGGC 
CTCAAGATGG CTGCCACCTT CATTGGCAAC AGCACGGCCA TTCAGGAGCT GTTCAAGCGC 
ATCTCAGAGC AGTTCACCGC CATGTTCCGC CGCAAGGCCT TCCTCCACTG GTACACCGGC 
GAGGGCATGG ATGAGATGGA GTTCACAGAG GCTGAGAGCA ACATGAACGA CCTGGTGTCT 
GAGTACCAGC AGTACCAGGA CGCCACCGCT GAGGAGGAGG GCGAGTTTGA GGATGAAGGC 
GAAGAGGACC TGGCCTAG