dph2 cDNA ORF clone, Nothobranchius furzeri(turquoise killifish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dph2
Entrez Gene ID	107382637
Full Name	DPH2 homolog
Gene Type	protein-coding
Organism	Nothobranchius furzeri(turquoise killifish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015954858.1	XP_015810344.1	diphthamide biosynthesis protein 2

Pathways from BioSystems
Gene Ontology

The following dph2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dph2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONo13081	XM_015954858.1 Latest version!	Nothobranchius furzeri DPH2 homolog (dph2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dph2 ( XM_015954858.1 ) cDNA ORF clone, Nothobranchius furzeri(turquoise killifish) -> XP_015810344.1 Nothobranchius furzeri DPH2 homolog (dph2), mRNA.

Price & Availability↑

CloneID	ONo13081
Clone ID Related Accession (Same CDS sequence)	XM_015954858.1
Accession Version	XM_015954858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-17
Organism	Nothobranchius furzeri(turquoise killifish)
Product	diphthamide biosynthesis protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015443549.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nothobranchius furzeri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG CGTTCAGTAG CAGCTCGGAA ACTGTAATTC GTCGTTCGAT AAACGTCGAA 
GTGAAGGCAA ATACCTCTGG GGAGAAACTG GAAGAGTTTT ATGACATTAA AGAGACATGC 
GAGTTTATTA GGGAGCATAA CTTTGACAAG GTTGCTCTGC AGTTTCCCGA TGAACTGCTG 
GTTGATTCGG TGGCCGTAGC CCAGGAGATT GAAAGAAACA GTCATGCCAA AACGTTTATT 
TTAGGAGACA CCTCTTATGG CAGCTGCTGT GTGGATGAAG TCGCAGCAGA GCACGTTGGA 
GCTTCCTGCA TTGTGCACTA TGGCAGTGCT TGCCTCAGCC CATCCAACAG GCTTCCTGTG 
TTGTACATCT TTGAAAAGAG ACCATTGGAT GTGGAGTTGT GCGCCTCCTC CTTCAGGGAG 
CTGTACCCCG ACACGCAAAC GCATGTCATC CTCCTCTGTG ATGTCAACTA TAATCACGCT 
ATTGATGGTC TTCTGGCACT TCTGTCACCA GAGTATCCAA ACCTTGCTGT CTCGGAGCTT 
GTTGTCGGCG GAGGACAGTG TTTCATTCAC GGAAGAAAAC AAATGGATAA AACTTGTCCT 
TCTGAGCCTG AAAACAGCCT GGTCATATCT CAGTTTGGGA GGCGGTTTTC CCTAAAATGT 
GGTACAGCTG TAACAGATTA CAGGATGTTT TATGTTGGCC TGGAGGGGGC GACCTTAAGG 
AACTTCATGA TGACTTGGAA TCGCTGCTCG TTTTGCTCTT TTGATCCCGT CTCACTGAAA 
GGAAGGACTG AGTCTATTAA TGTTAACCGA GCGCTGATGA AGCGATATTA CGCCATAGAA 
CGGGCCAAAG ATGCCAACGT GGTTGGTATT CTTGTCGGGA CCTTGGGTGT GGTTGACTAC 
CTCTCCATCA TCCAGCAGCT GAAGGAAACC ATTCACCGAG CTGGCAAGAA GAGCTACATG 
TTCCTCATGG GGAAACTCAA CGTACCCAAA CTAGCTAACT TTATGGAAAT CGACATATTT 
GTGCTCATCG CATGTCCGGA GAACTCGCTG TTGGACTCCA GTGAATTCTA TAAACCCATC 
GTAACGCCGT TTGAGATGGA AGTGGCCTGC AACAAGAAAA GGGAGTGGTC AGGGGAATAT 
GTCACGGACT TCCGACACCT CCTGCCAGGT GGTGGAAGTC ATGTGCCGTT AGCCGATCAG 
CAGGAGGACG TCGACGATGA GACAGACGTT TCATTGATCA CCGGAGCGCT GCGAAGCCAG 
AACCTGATGA ACAGCGAGCC TGTAGAGTCC TCGTTTGGAT CCTCTGTGGT CCTGAGGAAC 
CAGACTCTGA CCGTAGCCAA CGCCGACTCA GCAGCGTCCT TTTTGGCAGG TAGAAGCTGG 
CGTGGCTTAG AGCCGGCGCT GGGAGACGCT CCTGTGGTGA AGGTAGTAGA GGGAAGAAAA 
GGCATCGCTA TCGCCTACGA AGACGAAGGA ACATCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015810344.1
CDS	184..1662
Translation	MAEAFSSSSETVIRRSINVEVKANTSGEKLEEFYDIKETCEFIREHNFDKVALQFPDELLVDSVAVAQEIERNSHAKTFILGDTSYGSCCVDEVAAEHVGASCIVHYGSACLSPSNRLPVLYIFEKRPLDVELCASSFRELYPDTQTHVILLCDVNYNHAIDGLLALLSPEYPNLAVSELVVGGGQCFIHGRKQMDKTCPSEPENSLVISQFGRRFSLKCGTAVTDYRMFYVGLEGATLRNFMMTWNRCSFCSFDPVSLKGRTESINVNRALMKRYYAIERAKDANVVGILVGTLGVVDYLSIIQQLKETIHRAGKKSYMFLMGKLNVPKLANFMEIDIFVLIACPENSLLDSSEFYKPIVTPFEMEVACNKKREWSGEYVTDFRHLLPGGGSHVPLADQQEDVDDETDVSLITGALRSQNLMNSEPVESSFGSSVVLRNQTLTVANADSAASFLAGRSWRGLEPALGDAPVVKVVEGRKGIAIAYEDEGTS

Target ORF information:

RefSeq Version	XM_015954858.1
Organism	Nothobranchius furzeri(turquoise killifish)
Definition	Nothobranchius furzeri DPH2 homolog (dph2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015954858.1

ORF Insert Sequence:

ATGGCTGAGG CGTTCAGTAG CAGCTCGGAA ACTGTAATTC GTCGTTCGAT AAACGTCGAA 
GTGAAGGCAA ATACCTCTGG GGAGAAACTG GAAGAGTTTT ATGACATTAA AGAGACATGC 
GAGTTTATTA GGGAGCATAA CTTTGACAAG GTTGCTCTGC AGTTTCCCGA TGAACTGCTG 
GTTGATTCGG TGGCCGTAGC CCAGGAGATT GAAAGAAACA GTCATGCCAA AACGTTTATT 
TTAGGAGACA CCTCTTATGG CAGCTGCTGT GTGGATGAAG TCGCAGCAGA GCACGTTGGA 
GCTTCCTGCA TTGTGCACTA TGGCAGTGCT TGCCTCAGCC CATCCAACAG GCTTCCTGTG 
TTGTACATCT TTGAAAAGAG ACCATTGGAT GTGGAGTTGT GCGCCTCCTC CTTCAGGGAG 
CTGTACCCCG ACACGCAAAC GCATGTCATC CTCCTCTGTG ATGTCAACTA TAATCACGCT 
ATTGATGGTC TTCTGGCACT TCTGTCACCA GAGTATCCAA ACCTTGCTGT CTCGGAGCTT 
GTTGTCGGCG GAGGACAGTG TTTCATTCAC GGAAGAAAAC AAATGGATAA AACTTGTCCT 
TCTGAGCCTG AAAACAGCCT GGTCATATCT CAGTTTGGGA GGCGGTTTTC CCTAAAATGT 
GGTACAGCTG TAACAGATTA CAGGATGTTT TATGTTGGCC TGGAGGGGGC GACCTTAAGG 
AACTTCATGA TGACTTGGAA TCGCTGCTCG TTTTGCTCTT TTGATCCCGT CTCACTGAAA 
GGAAGGACTG AGTCTATTAA TGTTAACCGA GCGCTGATGA AGCGATATTA CGCCATAGAA 
CGGGCCAAAG ATGCCAACGT GGTTGGTATT CTTGTCGGGA CCTTGGGTGT GGTTGACTAC 
CTCTCCATCA TCCAGCAGCT GAAGGAAACC ATTCACCGAG CTGGCAAGAA GAGCTACATG 
TTCCTCATGG GGAAACTCAA CGTACCCAAA CTAGCTAACT TTATGGAAAT CGACATATTT 
GTGCTCATCG CATGTCCGGA GAACTCGCTG TTGGACTCCA GTGAATTCTA TAAACCCATC 
GTAACGCCGT TTGAGATGGA AGTGGCCTGC AACAAGAAAA GGGAGTGGTC AGGGGAATAT 
GTCACGGACT TCCGACACCT CCTGCCAGGT GGTGGAAGTC ATGTGCCGTT AGCCGATCAG 
CAGGAGGACG TCGACGATGA GACAGACGTT TCATTGATCA CCGGAGCGCT GCGAAGCCAG 
AACCTGATGA ACAGCGAGCC TGTAGAGTCC TCGTTTGGAT CCTCTGTGGT CCTGAGGAAC 
CAGACTCTGA CCGTAGCCAA CGCCGACTCA GCAGCGTCCT TTTTGGCAGG TAGAAGCTGG 
CGTGGCTTAG AGCCGGCGCT GGGAGACGCT CCTGTGGTGA AGGTAGTAGA GGGAAGAAAA 
GGCATCGCTA TCGCCTACGA AGACGAAGGA ACATCGTGA