LOC104103051 cDNA ORF clone, Nicotiana tomentosiformis

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104103051
Entrez Gene ID	104103051
Full Name	probable E3 ubiquitin-protein ligase RNF217
Gene Type	protein-coding
Organism	Nicotiana tomentosiformis

mRNA and Protein(s)

mRNA	Protein	Name
XM_009610919.2	XP_009609214.1	probable E3 ubiquitin-protein ligase RNF217

Pathways from BioSystems
Gene Ontology

The following LOC104103051 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104103051 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONi102359	XM_009610919.2 Latest version!	Nicotiana tomentosiformis probable E3 ubiquitin-protein ligase RNF217 (LOC104103051), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104103051 ( XM_009610919.2 ) cDNA ORF clone, Nicotiana tomentosiformis -> XP_009609214.1 Nicotiana tomentosiformis probable E3 ubiquitin-protein ligase RNF217 (LOC104103051), mRNA.

Price & Availability↑

CloneID	ONi102359
Clone ID Related Accession (Same CDS sequence)	XM_009610919.2
Accession Version	XM_009610919.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-18
Organism	Nicotiana tomentosiformis
Product	probable E3 ubiquitin-protein ligase RNF217
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_008890357.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 19, 2016 this sequence version replaced XM_009610919.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nicotiana tomentosiformis Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGATC GTATTGATGT CTTGGCTATG ATGATATCAG ATGAGGAATT TGCAGAGAAA 
CTGCAGATGC AACATGTTCT CGGAGCCTCT TATGAAAGTT CCCAAGGATT CTGTGGAATT 
TGCAATAAGA AAAAGGGAGC AGATCGAATG TTCAAACTCC AAATCTGCTC TCGTCATTCT 
TTCTGTTCTC ACTGCATAGG TCTTTATGCC CAATCCAAGA TTCGCGACAA CGTTTTCCCT 
ATAACTTGTC CAGGTTTGAG ATGTCGTGTC GTAATTCAAC CCGAATCTTG TAGGTCTATC 
ATTCCAGTAA ATACATTTGC AAGGTGGGAA GAGGGCTCGG CCGAGTCAGC TATTCCTAAT 
GGTGAAAAAT TCTATTGTCC TTATAAAAGA TGTTCAGGGC TGTTCATTCA TGATCGACAT 
GAAGAGATCG TTCAGTGCAT TTGTCCTCTG TGCAAAAAAC TGTTCTGTGC GAAGTGCCGA 
GTCCCTTGGC ACACTGGGCG TGATTGTGAC AAGTTTCAAA AGGTAGAAAA AGACAGAGAA 
GATGAGCTAA AACTTAAGCT GCTTGCTGAG ATCAAAAAAT GGAAGGAATG CCCCAAGTGC 
AAATCCATTG TGGAGAAGGT TGATGGCTGT ATACACATGA CTTGCAGGTG TAAAATGGAA 
TTTTGCTACG TATGTGGAGG AACGTGGAGC GAAAGGCATT GGAGTTGCCA AGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009609214.1
CDS	130..846
Translation	MSDRIDVLAMMISDEEFAEKLQMQHVLGASYESSQGFCGICNKKKGADRMFKLQICSRHSFCSHCIGLYAQSKIRDNVFPITCPGLRCRVVIQPESCRSIIPVNTFARWEEGSAESAIPNGEKFYCPYKRCSGLFIHDRHEEIVQCICPLCKKLFCAKCRVPWHTGRDCDKFQKVEKDREDELKLKLLAEIKKWKECPKCKSIVEKVDGCIHMTCRCKMEFCYVCGGTWSERHWSCQD

Target ORF information:

RefSeq Version	XM_009610919.2
Organism	Nicotiana tomentosiformis
Definition	Nicotiana tomentosiformis probable E3 ubiquitin-protein ligase RNF217 (LOC104103051), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009610919.2

ORF Insert Sequence:

ATGTCAGATC GTATTGATGT CTTGGCTATG ATGATATCAG ATGAGGAATT TGCAGAGAAA 
CTGCAGATGC AACATGTTCT CGGAGCCTCT TATGAAAGTT CCCAAGGATT CTGTGGAATT 
TGCAATAAGA AAAAGGGAGC AGATCGAATG TTCAAACTCC AAATCTGCTC TCGTCATTCT 
TTCTGTTCTC ACTGCATAGG TCTTTATGCC CAATCCAAGA TTCGCGACAA CGTTTTCCCT 
ATAACTTGTC CAGGTTTGAG ATGTCGTGTC GTAATTCAAC CCGAATCTTG TAGGTCTATC 
ATTCCAGTAA ATACATTTGC AAGGTGGGAA GAGGGCTCGG CCGAGTCAGC TATTCCTAAT 
GGTGAAAAAT TCTATTGTCC TTATAAAAGA TGTTCAGGGC TGTTCATTCA TGATCGACAT 
GAAGAGATCG TTCAGTGCAT TTGTCCTCTG TGCAAAAAAC TGTTCTGTGC GAAGTGCCGA 
GTCCCTTGGC ACACTGGGCG TGATTGTGAC AAGTTTCAAA AGGTAGAAAA AGACAGAGAA 
GATGAGCTAA AACTTAAGCT GCTTGCTGAG ATCAAAAAAT GGAAGGAATG CCCCAAGTGC 
AAATCCATTG TGGAGAAGGT TGATGGCTGT ATACACATGA CTTGCAGGTG TAAAATGGAA 
TTTTGCTACG TATGTGGAGG AACGTGGAGC GAAAGGCATT GGAGTTGCCA AGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.