NCU08603 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU08603
Entrez Gene ID	3880184
Full Name	ankyrin repeat protein
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_958942.3	XP_964035.3	ankyrin repeat protein

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_05656	XP_003710549.1
Neurospora crassa	NCU08603	XP_964035.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU08603 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU08603 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf12654	XM_958942.3 Latest version!	Neurospora crassa OR74A ankyrin repeat protein (NCU08603), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU08603 ( XM_958942.3 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_964035.3 Neurospora crassa OR74A ankyrin repeat protein (NCU08603), mRNA.

Price & Availability↑

CloneID	ONf12654
Clone ID Related Accession (Same CDS sequence)	XM_958942.3
Accession Version	XM_958942.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	ankyrin repeat protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_958942.2.

ATGGCGACGT CAACCTTCCC CCAGCTGCCC GTCCCTCATA GCGACCTGGT CAAGCACATC 
ACCAACCACC CACAGACCCC CATGGTCGAC CTCCTCCAGC CCTACCGTGA CTATGAGGCT 
CGTCTTCGTC AGGTCTACGC CCAAGAGCCC GACTCTGAGC TGCTCAAGGA CCCCTACGTC 
AACGTCCTGC CTCTCTTCAC AGACAACACC TCCGACATCA AGATCCGGGC CCGTAACATG 
GAGGCCGAGT CCGAAGAGGA GAGAGCACGC TACATCATGC CCCTGCCCAA GGACATACGC 
CGTCCAGACC AATCCCCCGC CGTTGTCCAG TCACACAAGG ACTTCCAGAG GAACTTCAAC 
ATCTTCTCCG AGTCTTCTCT GGTCGAGTTG AATTGGGACA ATGTCGTTGC GGCCGGCTCC 
TCGGTTGTCA ACTGCATCTT GCCGGTTCCC AAAAAGTACA GCGGCTCCAA GAGGCTGATG 
CGCGAGTTCT ACCATGAAAA GTTTGCTCCT GCGTCCGACG TCGATCTGTT CTTGTATGGC 
CTGACCGAGG CGCAGGCGAT TGAGAAGATC AAGGAGATTG AGAGCCAGGT CAGGGATGCG 
TTGCTTTGCG AGACTACCAC GGTTAGGACC AAGCATGCTG TTACCATTTG CAGCCAGTAT 
CCCACCAGAC ACATCCAGAT CGTCCTTCGC ATCTACAAGT CCGTGTCCGA AATTCTGACC 
GGTTTCGACA TCGACTGCTC TGGCGCTGCC TATGATGGAA AGCAAGTCTA TTGCACCCCT 
CGCGCTCTGC AGTCCTACAT GACCCAGACC AACCACATCG ACCTCACTCG CCGCAGTCCT 
TCCTACGAAA ACCGCCTTTC CAAGTACAGC CGCAGAGGGT TTGAGGTCTA CTGGCCTGAG 
CTTGACCGTT CTCGCATCGA CCCTACCATC TTCGAGCGGA GTTTTATGAG AACACTGGGT 
CTCGCACGAC TTCTCGTGCT GGAAAGACTG CCCACTGCCA AGGCTAGAGA CAACTATGTC 
GACCAGCGTC GCCAGGAAAG AGGTCGCCCT GCTCTTAATC GATTCGTGCG CAAGAACTTT 
CGGGCCATGG GTTCGAACAT CAAGGACGAC TACGACGACG AAATCGCCGA TTGGGTCAGC 
GGAGACGAAG TGTCCGACTA CCACACATTT ACCATCCCCT ACGGTCCCAA GTTCCACGCC 
AAAAAGATTG AGAAGCTTTG CTACACCAAG GATCTCTTGC TCAATGCCGA GTGGAACCAG 
CACGATAATC GGACCGTGTA CTTGCACCGC CATCCTGCCT TCTTTGGCCG CTTCAAGGAC 
GTCGTTGAAG ACTGCTGCGG ACACTGCCCG GTTCCCCAGA CCGATGAGGA GAAAGACATT 
GCCGAAGAGG AAGAGAAGAT CTACGTCTCT GGCAGAATCT CCTTCATCAA GGACGACCCC 
GGAAGACAGC AGATTGGCTC CTTCAACCCT TTGACCGATG ATGACTGGAC GGAAATGGCC 
TATATCGGCA ACACTGCCAG GCTGTGCCAG GCCATTGTCG ACGAGGATGT TGAGCATGTC 
CAAGACTGGC TTGCTCAGGA GGGCGCCAAT CCCAACACCA GAGACCACAC AGGCAGGACT 
CCCCTTCACC TTGCTGTCAT GTCTTCCTCG CCCGCAGTGG TTCGTGCCCT CGTTGAGGCT 
GGTGCCCGCC TTACTGCGCG TCTCGCGGAC GGAAGAACCG CACTCCACCT GGCCGCTTCT 
CGTGGCGACG TGGAAATTGT CAAGATCCTC ATGAATAAGA GCAACGCCAA CGAGGAAGAG 
TTTGAGGAAC TGGAAGATCA ACGTCGTAAG GCTAGGGCTG CACTTCGAGA GAAGAAGACG 
GAGAAGATGG ATATCGACGA GGAGAAGGCG GATGACTCGG AAGACATCGA AATGATCGAA 
GCAGGCGATG CCGAGTCTGA TGCGGGTGGC GATAAGATGA GCATCGCGAC AGGTTCGTTT 
GTCAAGGTTG ACAAGACTCC TGAAACACCC GCACCTGATG GCATACTCGT AGCCGAAGAT 
GAGGAAGGCC CTGACTTCTA CAAAGTGGAT GAAGTTGCAT GGGATATTCC GTGCTCGGCT 
TTGCACCTTG CCATCATGGA GGGGCACTGT GAAGTTGTCA AGCTTTTGTG CCAAGAATAC 
GGAGCTGATG TCTTGCTGCC CGTCAAGTTC CCTCAGGATA AGAATAAGGT CCTCCTGACG 
CTGGGTCTTG TCATGGCCCT TCCCATCGAA AAGGCAAAGT CGATGGCTCA GACGCTTCTC 
AGCCTTGGTG CCACGGTCGC CCAGGCGGAC ATGAACAGTT GCACTGTGTT CACCCGTTAC 
GTGGAGCGAA ATGCCAGGAC GCTGCTGGAC TTGGCCTGTG AGCTCGACAA GGCAGGCTAC 
AAGACGGCAA TCAACCACGT CTGCAATCAA TGGGCCCAAA GTGAGACGCC ATTGCTAATC 
GCAATCAAAG ATGGCGACCT GAGCATGGTT GTGAAGCTCC TTGAGACGGG CGCTGTGCCA 
GAGATTGACT TTGAGACCTG GCTCAAGAGC GCCAGACAAT CGCCCAAGGA GAGCCGGCTC 
AGCGACTTTG AGAGCAACAA GAAATTGTTC CAAGAAGATC TTGATCGCCC ATTGATTGCT 
GCCTTGCGGT CCAAGGAGCC CAGCATTGCG CTTGAACTTT TAGAACGGGG GTGCGATCCG 
AATCCCCTCC ACCCCGAGTC AGTAAGGGGT TTGCATAGTA CTTACTCCTA TCGATGGAAG 
GGCAAAAGTG CCCTTGACAT GGTGCGGGAG CAGCTAGAGG ATCTTCGAAA ATACAAAGGA 
GAAAACACCA AATTCGAGGA GCCCAAGCCG CGCCTGGGAC TTGACACGTA TCTCCAACGC 
TTCGAGGAGG GCACTTATCA ACACTGGGCG GTATCCGGCG GAGTCAAAGT TGAGAAGATG 
GGGGACCCGG AGAAGCGGAC GGAATATGAG ATAGGTCTAA AATGTCTTGA GAGAACGAAG 
GGCATTCCTG AAAAGGAGCA GGCCGTCCGA GACGCCATTG CGACGCTGGA GAGAATAGAA 
CAGAGCATCA TCTCTAAGGG TGGCAAGACG TTCAAGGAGC TCTATCCCGA CCGCAAGGAG 
GACGGTAATG ATGACACCAG CATCAATCAC CATGGGTTCT GGCTTGGTCG TCATCATGAC 
GACGAGGTTT CCAAGAAAGA GCCGTATCAC TATAACTTCG TGTTCACCGG TGTTCAGGAC 
ATGACTGAGA CAAAGCAGGC TGCCTACATG GAGCTTTTTG AGGCTGTATG GACTGGTGAT 
CTCGATAAGG TCAAAAAATT CACCTTGACT TCTTGGGACG ACGCCAAGCA GCAGCCGCCA 
CTAAAGATTG CTGTCGAAGG TCACAATGGA CACAATCCCT TCACCCTGGC GTTGCAGTGT 
GGTCACTACG AACTCGCCAA GGCGGTGCTA GAGATTGCTC AGGCGCAGTA CTCACCTCCG 
GAGAAGGCCA AGACCCGTTA CTCGATGAAC AAGAGCGGCG GGGAAGAAGA AGAAGATGAT 
GATGATGATA GTTCCTCCGA CGAGTCTGCA CCCAGAATTG TGGGCGAGAC TGTCGACGAT 
CAGCTTACCA TTGACACCAT CGGTCAGGTG TCCATGCAGG TAGAGAGTCA CACCTTACCA 
GTATCTCTGC TTAGGGGCAG GACGGCCACC AACGGTCTCA ACTACGGCAG ATGGCGTAAT 
CACATGCAGT TGAACAAGGG CCAGGTTCTC GATGATGAAG AGGAAACGTG GGATCCGCTG 
ATGTATGCCG TCACGAATAA CGACTTGAAG CTCCTTAAGT TTGTGCTCGA TATGGGCGAG 
TACTTTGGAA CCCTGCAGCT TGACCCGGCC ACAGAGCCTC CCAAGTTCTT CTCCGCACCA 
GATGAAGTCT TTGCCACGGC CATTCGGAAG GGACGCGTTG AGGTCATGGC TGAGCTGATC 
AGACGCACGG GTGCGGGCTT GCCATTGGAG CACCTGGTGG AAGGATCCGG TGTCGAGCTT 
AAGGAGCAGC CGCGCTATTA CCAGGGCTTG TCGGTGTATG GAAAGAAGAG AAAGGATTGG 
GCCAATGCCG GCCGGGAAGT GGCTCAACAA CCACGCGATG AGAATTCCAC AACGTCGCCT 
CTGCTGAGAG CTGCTGAGAG CTGTTGCATC GAGAGTATCC AGTGGTTCTT GAGCGATGCC 
CCGTTGAGAC ATTACCTCGA GTTTGCCAAA TCCAAGGCTG CTCAGGAAGA CACCAGGATG 
CAACACCTGG GACGGGCCCC CGGAGGTGTT GAAGGTGCGA TCGCTAGATG GCTTGATGAT 
CAGGGCGAGT TGATCATCCA CGCCGTCATT GTCAAGTCTG GCCAAGTCGA CGTTAGTCAT 
CTGGTCTCGT ATCTTATCAA GGCTTGCCCT GAAGCCTTGG AGGCCAAGTC ACACAACGTG 
ACACCGCTGG TGCAGGCCGT CTACATGGGT CGTGTGGAAC TCGTCAAGCT CCTGCTAGAA 
GCCGGTGCTG ACCCAAACGT CAGGACTAGC TCGTGGACCA ATATTTTGCA CGCCGCATTG 
CTCTACACGC CACCGGCTGA CAAGCTCAAG GAACTGCTAA GCCTCTTCGA CCGCGAGGCC 
GTCATCCGCA TGGTCACTGA GCGTTCCAGC CTGGCCAGTG ATGGCCGCAC CCCACTCCAC 
CAATGGCTGA CCTGCATCGG AGGGAACGAA GACCACAACA AGTCCGTCGC CGTCTTCAAC 
CTCCTCGTTG AGATGTCGTC TGAGGCGGTG ACCACCGCGC TCAATATGCT TGACGCTTCC 
GGTGACACGC CTTTGCACGA CATGTTAACC TCACACACCA GCTCCAAGCT GATCCGGGCC 
GTGCTGGACT TCCAACCCAG CCTGCTCTTC CGTGAGAACG CCGTGGGCCG GACCCCCGTC 
GAAGTGGTTC ACGACGAGCT CATTTCCAGC CGAGTCAAGT CAGAGAGCGG ACAGCAGGGG 
CGCTACCATT CAAGGTACAG TGTGTCTGGA GCTGAAGGCT ACCGCTACTT GATGGGAGAG 
AAGCCGCAGG TGTTTCTCAA CAACATGAAA CTTTATCAGG AAAAACGGAG CCGTGAGCAT 
GAGAGCCAAT ATAGTGACAG CCATAATACC CGAATGTGGG CTCTTTGCCA GGAATATTTG 
GCCAAATTGG CCAGCACCCC TCCTCCATCT CGTTCCGCTT CACCTCCCAC CACCGAAGGC 
CAAACCCAAG CTCATCATGC CCGCGACCAG TCGCCTCCCA AACGCCGCAA GCTCACGACA 
GAAACCGAAG GCCGTCTTAA CGCACACATC ATCGCCACCC ACAAGCTGAA AGAGAAGCGC 
ACACTCGTCT CTCTCCCCGA AGCCAACGAC GTAGCCAAGC GTCTGGGCGA AATGAAGGTG 
GGATCCAAGT ACAATTACCA ACTTGCTCCT CCCGAAGAGG AAGGTCAAGA AGAAGAAGAT 
GTGAAACGGT TCGCAGACGA CGAGACCCCT TTCGCCTCCA AGCCCGACCG TCGCACATGG 
GGAGACGGTG TCCGTTATGG CAGGTGGAGA AAGCAAAATG GAGAGAAGCA GACGCCGCGG 
AAGAGTTATC GTGTACTGGC CAATGTGGCT TGGAAGGATC TGAAGAAGAG TGGGGAGAAG 
GCTGATGACG GGCTGGATTG GGGACGGTGT AGGTTTTGCG GGGATTATCA TACGAAGTCT 
GGGGAGCCGC ATCCGTACAA CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_964035.3
CDS	466..6192
Translation	MATSTFPQLPVPHSDLVKHITNHPQTPMVDLLQPYRDYEARLRQVYAQEPDSELLKDPYVNVLPLFTDNTSDIKIRARNMEAESEEERARYIMPLPKDIRRPDQSPAVVQSHKDFQRNFNIFSESSLVELNWDNVVAAGSSVVNCILPVPKKYSGSKRLMREFYHEKFAPASDVDLFLYGLTEAQAIEKIKEIESQVRDALLCETTTVRTKHAVTICSQYPTRHIQIVLRIYKSVSEILTGFDIDCSGAAYDGKQVYCTPRALQSYMTQTNHIDLTRRSPSYENRLSKYSRRGFEVYWPELDRSRIDPTIFERSFMRTLGLARLLVLERLPTAKARDNYVDQRRQERGRPALNRFVRKNFRAMGSNIKDDYDDEIADWVSGDEVSDYHTFTIPYGPKFHAKKIEKLCYTKDLLLNAEWNQHDNRTVYLHRHPAFFGRFKDVVEDCCGHCPVPQTDEEKDIAEEEEKIYVSGRISFIKDDPGRQQIGSFNPLTDDDWTEMAYIGNTARLCQAIVDEDVEHVQDWLAQEGANPNTRDHTGRTPLHLAVMSSSPAVVRALVEAGARLTARLADGRTALHLAASRGDVEIVKILMNKSNANEEEFEELEDQRRKARAALREKKTEKMDIDEEKADDSEDIEMIEAGDAESDAGGDKMSIATGSFVKVDKTPETPAPDGILVAEDEEGPDFYKVDEVAWDIPCSALHLAIMEGHCEVVKLLCQEYGADVLLPVKFPQDKNKVLLTLGLVMALPIEKAKSMAQTLLSLGATVAQADMNSCTVFTRYVERNARTLLDLACELDKAGYKTAINHVCNQWAQSETPLLIAIKDGDLSMVVKLLETGAVPEIDFETWLKSARQSPKESRLSDFESNKKLFQEDLDRPLIAALRSKEPSIALELLERGCDPNPLHPESVRGLHSTYSYRWKGKSALDMVREQLEDLRKYKGENTKFEEPKPRLGLDTYLQRFEEGTYQHWAVSGGVKVEKMGDPEKRTEYEIGLKCLERTKGIPEKEQAVRDAIATLERIEQSIISKGGKTFKELYPDRKEDGNDDTSINHHGFWLGRHHDDEVSKKEPYHYNFVFTGVQDMTETKQAAYMELFEAVWTGDLDKVKKFTLTSWDDAKQQPPLKIAVEGHNGHNPFTLALQCGHYELAKAVLEIAQAQYSPPEKAKTRYSMNKSGGEEEEDDDDDSSSDESAPRIVGETVDDQLTIDTIGQVSMQVESHTLPVSLLRGRTATNGLNYGRWRNHMQLNKGQVLDDEEETWDPLMYAVTNNDLKLLKFVLDMGEYFGTLQLDPATEPPKFFSAPDEVFATAIRKGRVEVMAELIRRTGAGLPLEHLVEGSGVELKEQPRYYQGLSVYGKKRKDWANAGREVAQQPRDENSTTSPLLRAAESCCIESIQWFLSDAPLRHYLEFAKSKAAQEDTRMQHLGRAPGGVEGAIARWLDDQGELIIHAVIVKSGQVDVSHLVSYLIKACPEALEAKSHNVTPLVQAVYMGRVELVKLLLEAGADPNVRTSSWTNILHAALLYTPPADKLKELLSLFDREAVIRMVTERSSLASDGRTPLHQWLTCIGGNEDHNKSVAVFNLLVEMSSEAVTTALNMLDASGDTPLHDMLTSHTSSKLIRAVLDFQPSLLFRENAVGRTPVEVVHDELISSRVKSESGQQGRYHSRYSVSGAEGYRYLMGEKPQVFLNNMKLYQEKRSREHESQYSDSHNTRMWALCQEYLAKLASTPPPSRSASPPTTEGQTQAHHARDQSPPKRRKLTTETEGRLNAHIIATHKLKEKRTLVSLPEANDVAKRLGEMKVGSKYNYQLAPPEEEGQEEEDVKRFADDETPFASKPDRRTWGDGVRYGRWRKQNGEKQTPRKSYRVLANVAWKDLKKSGEKADDGLDWGRCRFCGDYHTKSGEPHPYNH

Target ORF information:

RefSeq Version	XM_958942.3
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A ankyrin repeat protein (NCU08603), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_958942.3

ORF Insert Sequence:

ATGGCGACGT CAACCTTCCC CCAGCTGCCC GTCCCTCATA GCGACCTGGT CAAGCACATC 
ACCAACCACC CACAGACCCC CATGGTCGAC CTCCTCCAGC CCTACCGTGA CTATGAGGCT 
CGTCTTCGTC AGGTCTACGC CCAAGAGCCC GACTCTGAGC TGCTCAAGGA CCCCTACGTC 
AACGTCCTGC CTCTCTTCAC AGACAACACC TCCGACATCA AGATCCGGGC CCGTAACATG 
GAGGCCGAGT CCGAAGAGGA GAGAGCACGC TACATCATGC CCCTGCCCAA GGACATACGC 
CGTCCAGACC AATCCCCCGC CGTTGTCCAG TCACACAAGG ACTTCCAGAG GAACTTCAAC 
ATCTTCTCCG AGTCTTCTCT GGTCGAGTTG AATTGGGACA ATGTCGTTGC GGCCGGCTCC 
TCGGTTGTCA ACTGCATCTT GCCGGTTCCC AAAAAGTACA GCGGCTCCAA GAGGCTGATG 
CGCGAGTTCT ACCATGAAAA GTTTGCTCCT GCGTCCGACG TCGATCTGTT CTTGTATGGC 
CTGACCGAGG CGCAGGCGAT TGAGAAGATC AAGGAGATTG AGAGCCAGGT CAGGGATGCG 
TTGCTTTGCG AGACTACCAC GGTTAGGACC AAGCATGCTG TTACCATTTG CAGCCAGTAT 
CCCACCAGAC ACATCCAGAT CGTCCTTCGC ATCTACAAGT CCGTGTCCGA AATTCTGACC 
GGTTTCGACA TCGACTGCTC TGGCGCTGCC TATGATGGAA AGCAAGTCTA TTGCACCCCT 
CGCGCTCTGC AGTCCTACAT GACCCAGACC AACCACATCG ACCTCACTCG CCGCAGTCCT 
TCCTACGAAA ACCGCCTTTC CAAGTACAGC CGCAGAGGGT TTGAGGTCTA CTGGCCTGAG 
CTTGACCGTT CTCGCATCGA CCCTACCATC TTCGAGCGGA GTTTTATGAG AACACTGGGT 
CTCGCACGAC TTCTCGTGCT GGAAAGACTG CCCACTGCCA AGGCTAGAGA CAACTATGTC 
GACCAGCGTC GCCAGGAAAG AGGTCGCCCT GCTCTTAATC GATTCGTGCG CAAGAACTTT 
CGGGCCATGG GTTCGAACAT CAAGGACGAC TACGACGACG AAATCGCCGA TTGGGTCAGC 
GGAGACGAAG TGTCCGACTA CCACACATTT ACCATCCCCT ACGGTCCCAA GTTCCACGCC 
AAAAAGATTG AGAAGCTTTG CTACACCAAG GATCTCTTGC TCAATGCCGA GTGGAACCAG 
CACGATAATC GGACCGTGTA CTTGCACCGC CATCCTGCCT TCTTTGGCCG CTTCAAGGAC 
GTCGTTGAAG ACTGCTGCGG ACACTGCCCG GTTCCCCAGA CCGATGAGGA GAAAGACATT 
GCCGAAGAGG AAGAGAAGAT CTACGTCTCT GGCAGAATCT CCTTCATCAA GGACGACCCC 
GGAAGACAGC AGATTGGCTC CTTCAACCCT TTGACCGATG ATGACTGGAC GGAAATGGCC 
TATATCGGCA ACACTGCCAG GCTGTGCCAG GCCATTGTCG ACGAGGATGT TGAGCATGTC 
CAAGACTGGC TTGCTCAGGA GGGCGCCAAT CCCAACACCA GAGACCACAC AGGCAGGACT 
CCCCTTCACC TTGCTGTCAT GTCTTCCTCG CCCGCAGTGG TTCGTGCCCT CGTTGAGGCT 
GGTGCCCGCC TTACTGCGCG TCTCGCGGAC GGAAGAACCG CACTCCACCT GGCCGCTTCT 
CGTGGCGACG TGGAAATTGT CAAGATCCTC ATGAATAAGA GCAACGCCAA CGAGGAAGAG 
TTTGAGGAAC TGGAAGATCA ACGTCGTAAG GCTAGGGCTG CACTTCGAGA GAAGAAGACG 
GAGAAGATGG ATATCGACGA GGAGAAGGCG GATGACTCGG AAGACATCGA AATGATCGAA 
GCAGGCGATG CCGAGTCTGA TGCGGGTGGC GATAAGATGA GCATCGCGAC AGGTTCGTTT 
GTCAAGGTTG ACAAGACTCC TGAAACACCC GCACCTGATG GCATACTCGT AGCCGAAGAT 
GAGGAAGGCC CTGACTTCTA CAAAGTGGAT GAAGTTGCAT GGGATATTCC GTGCTCGGCT 
TTGCACCTTG CCATCATGGA GGGGCACTGT GAAGTTGTCA AGCTTTTGTG CCAAGAATAC 
GGAGCTGATG TCTTGCTGCC CGTCAAGTTC CCTCAGGATA AGAATAAGGT CCTCCTGACG 
CTGGGTCTTG TCATGGCCCT TCCCATCGAA AAGGCAAAGT CGATGGCTCA GACGCTTCTC 
AGCCTTGGTG CCACGGTCGC CCAGGCGGAC ATGAACAGTT GCACTGTGTT CACCCGTTAC 
GTGGAGCGAA ATGCCAGGAC GCTGCTGGAC TTGGCCTGTG AGCTCGACAA GGCAGGCTAC 
AAGACGGCAA TCAACCACGT CTGCAATCAA TGGGCCCAAA GTGAGACGCC ATTGCTAATC 
GCAATCAAAG ATGGCGACCT GAGCATGGTT GTGAAGCTCC TTGAGACGGG CGCTGTGCCA 
GAGATTGACT TTGAGACCTG GCTCAAGAGC GCCAGACAAT CGCCCAAGGA GAGCCGGCTC 
AGCGACTTTG AGAGCAACAA GAAATTGTTC CAAGAAGATC TTGATCGCCC ATTGATTGCT 
GCCTTGCGGT CCAAGGAGCC CAGCATTGCG CTTGAACTTT TAGAACGGGG GTGCGATCCG 
AATCCCCTCC ACCCCGAGTC AGTAAGGGGT TTGCATAGTA CTTACTCCTA TCGATGGAAG 
GGCAAAAGTG CCCTTGACAT GGTGCGGGAG CAGCTAGAGG ATCTTCGAAA ATACAAAGGA 
GAAAACACCA AATTCGAGGA GCCCAAGCCG CGCCTGGGAC TTGACACGTA TCTCCAACGC 
TTCGAGGAGG GCACTTATCA ACACTGGGCG GTATCCGGCG GAGTCAAAGT TGAGAAGATG 
GGGGACCCGG AGAAGCGGAC GGAATATGAG ATAGGTCTAA AATGTCTTGA GAGAACGAAG 
GGCATTCCTG AAAAGGAGCA GGCCGTCCGA GACGCCATTG CGACGCTGGA GAGAATAGAA 
CAGAGCATCA TCTCTAAGGG TGGCAAGACG TTCAAGGAGC TCTATCCCGA CCGCAAGGAG 
GACGGTAATG ATGACACCAG CATCAATCAC CATGGGTTCT GGCTTGGTCG TCATCATGAC 
GACGAGGTTT CCAAGAAAGA GCCGTATCAC TATAACTTCG TGTTCACCGG TGTTCAGGAC 
ATGACTGAGA CAAAGCAGGC TGCCTACATG GAGCTTTTTG AGGCTGTATG GACTGGTGAT 
CTCGATAAGG TCAAAAAATT CACCTTGACT TCTTGGGACG ACGCCAAGCA GCAGCCGCCA 
CTAAAGATTG CTGTCGAAGG TCACAATGGA CACAATCCCT TCACCCTGGC GTTGCAGTGT 
GGTCACTACG AACTCGCCAA GGCGGTGCTA GAGATTGCTC AGGCGCAGTA CTCACCTCCG 
GAGAAGGCCA AGACCCGTTA CTCGATGAAC AAGAGCGGCG GGGAAGAAGA AGAAGATGAT 
GATGATGATA GTTCCTCCGA CGAGTCTGCA CCCAGAATTG TGGGCGAGAC TGTCGACGAT 
CAGCTTACCA TTGACACCAT CGGTCAGGTG TCCATGCAGG TAGAGAGTCA CACCTTACCA 
GTATCTCTGC TTAGGGGCAG GACGGCCACC AACGGTCTCA ACTACGGCAG ATGGCGTAAT 
CACATGCAGT TGAACAAGGG CCAGGTTCTC GATGATGAAG AGGAAACGTG GGATCCGCTG 
ATGTATGCCG TCACGAATAA CGACTTGAAG CTCCTTAAGT TTGTGCTCGA TATGGGCGAG 
TACTTTGGAA CCCTGCAGCT TGACCCGGCC ACAGAGCCTC CCAAGTTCTT CTCCGCACCA 
GATGAAGTCT TTGCCACGGC CATTCGGAAG GGACGCGTTG AGGTCATGGC TGAGCTGATC 
AGACGCACGG GTGCGGGCTT GCCATTGGAG CACCTGGTGG AAGGATCCGG TGTCGAGCTT 
AAGGAGCAGC CGCGCTATTA CCAGGGCTTG TCGGTGTATG GAAAGAAGAG AAAGGATTGG 
GCCAATGCCG GCCGGGAAGT GGCTCAACAA CCACGCGATG AGAATTCCAC AACGTCGCCT 
CTGCTGAGAG CTGCTGAGAG CTGTTGCATC GAGAGTATCC AGTGGTTCTT GAGCGATGCC 
CCGTTGAGAC ATTACCTCGA GTTTGCCAAA TCCAAGGCTG CTCAGGAAGA CACCAGGATG 
CAACACCTGG GACGGGCCCC CGGAGGTGTT GAAGGTGCGA TCGCTAGATG GCTTGATGAT 
CAGGGCGAGT TGATCATCCA CGCCGTCATT GTCAAGTCTG GCCAAGTCGA CGTTAGTCAT 
CTGGTCTCGT ATCTTATCAA GGCTTGCCCT GAAGCCTTGG AGGCCAAGTC ACACAACGTG 
ACACCGCTGG TGCAGGCCGT CTACATGGGT CGTGTGGAAC TCGTCAAGCT CCTGCTAGAA 
GCCGGTGCTG ACCCAAACGT CAGGACTAGC TCGTGGACCA ATATTTTGCA CGCCGCATTG 
CTCTACACGC CACCGGCTGA CAAGCTCAAG GAACTGCTAA GCCTCTTCGA CCGCGAGGCC 
GTCATCCGCA TGGTCACTGA GCGTTCCAGC CTGGCCAGTG ATGGCCGCAC CCCACTCCAC 
CAATGGCTGA CCTGCATCGG AGGGAACGAA GACCACAACA AGTCCGTCGC CGTCTTCAAC 
CTCCTCGTTG AGATGTCGTC TGAGGCGGTG ACCACCGCGC TCAATATGCT TGACGCTTCC 
GGTGACACGC CTTTGCACGA CATGTTAACC TCACACACCA GCTCCAAGCT GATCCGGGCC 
GTGCTGGACT TCCAACCCAG CCTGCTCTTC CGTGAGAACG CCGTGGGCCG GACCCCCGTC 
GAAGTGGTTC ACGACGAGCT CATTTCCAGC CGAGTCAAGT CAGAGAGCGG ACAGCAGGGG 
CGCTACCATT CAAGGTACAG TGTGTCTGGA GCTGAAGGCT ACCGCTACTT GATGGGAGAG 
AAGCCGCAGG TGTTTCTCAA CAACATGAAA CTTTATCAGG AAAAACGGAG CCGTGAGCAT 
GAGAGCCAAT ATAGTGACAG CCATAATACC CGAATGTGGG CTCTTTGCCA GGAATATTTG 
GCCAAATTGG CCAGCACCCC TCCTCCATCT CGTTCCGCTT CACCTCCCAC CACCGAAGGC 
CAAACCCAAG CTCATCATGC CCGCGACCAG TCGCCTCCCA AACGCCGCAA GCTCACGACA 
GAAACCGAAG GCCGTCTTAA CGCACACATC ATCGCCACCC ACAAGCTGAA AGAGAAGCGC 
ACACTCGTCT CTCTCCCCGA AGCCAACGAC GTAGCCAAGC GTCTGGGCGA AATGAAGGTG 
GGATCCAAGT ACAATTACCA ACTTGCTCCT CCCGAAGAGG AAGGTCAAGA AGAAGAAGAT 
GTGAAACGGT TCGCAGACGA CGAGACCCCT TTCGCCTCCA AGCCCGACCG TCGCACATGG 
GGAGACGGTG TCCGTTATGG CAGGTGGAGA AAGCAAAATG GAGAGAAGCA GACGCCGCGG 
AAGAGTTATC GTGTACTGGC CAATGTGGCT TGGAAGGATC TGAAGAAGAG TGGGGAGAAG 
GCTGATGACG GGCTGGATTG GGGACGGTGT AGGTTTTGCG GGGATTATCA TACGAAGTCT 
GGGGAGCCGC ATCCGTACAA CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU08603 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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