chs-4 cDNA ORF clone, Neurospora crassa OR74A

The following chs-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chs-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf02489 XM_011394973.1
Latest version!
Neurospora crassa OR74A chitin synthase 4 (chs-4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ONf02489 XM_011394972.1
Latest version!
Neurospora crassa OR74A chitin synthase 4, variant (chs-4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf02489
    Clone ID Related Accession (Same CDS sequence) XM_011394972.1 , XM_011394973.1
    Accession Version XM_011394972.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product chitin synthase 4, variant
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCTTC CTCGGAGGCC GGGGCCGTCG CCTCCGCACG AGGAGCCACG CCGCTACCGG 
    CAAAGCGGTT CGCGCCGCAG TCGACCACCA CCCGCCGATG TTGAGACCGG ATATGCTCCC
    ATGGCAGGAG AAAGACCAAG CCAGCAGCAA CGCGTACCAT CGATATCTTC ATTTCCGGAA
    ACCCTCCCGT CCCCCAACCC CAATGTCGAA CGAGACCCTT TGGCGCCCAC CCCCGAGCCC
    GCACATCCAT CCGGCATACC TCAGCGGAAG AGGAGTTTGA TTCGACCGGA GCGCAACAGG
    ATTGGCAAAG ACCACCCCAA CTATCACTAC AGAAAACATG CGGCCAACAT GAACACCCTA
    CCGTCCTCAA CCGGACACGA CCCGATATAC GAGGATCTGG AGGGAGCAAC GGACGATGTA
    TCGGGAACTG GCTCGAGGAA CGACGACGAT GTTTCCGAGG AGTCGCCCCC AAGAAGAAAG
    CACTCGACGA AAATGCAGGT CATCGAAACT GAAAAGTCCG GTGATGAAAG ACGACGTCGG
    CGGAAATCGG ATACCACAAA ACATGGTAAA ATTGTCAAGG CCTCCAAGGG AAAGCGCGAA
    AAGTCCGGAG GACTCCCTAC CCCGAGCTTT TGGAACATTT ACTGCGGATT CGTCACCTTC
    TGGTGCCCTG GCTTTGTCCT CAAGTGCTTC GGCATGCCCG AGATGGCTCA ACAGAGGGCG
    TGGAGAGAGA AGATGGGTCT GATCAGCATT ATTCTGCTCA TCATGGGCTT TGTTGGCTTC
    ATCACTTTCG GCTTCACCCA GGTGGTCTGC GGTAAACCTC CGCTCCGTTT GCGCATCAAT
    GAGGTCGGGT CAGGATACAT GATCTTCCAC GGATCTGCAT ATGACCTCAC CAAATCTCAC
    CATCCTCCGG CCGAGGGTAT CCCGCGCCGT CCGGACGGTT TGGGTGCAAA CGTGATCTAT
    GACTTGCCTC AGCATTACGG TGGACAGGAC GGCAGTTTCT TGTTCCAGAA CGTCAACGGC
    AAGTGCAAGG GTCTCATCAC CAAGCAAGAG AACTCCGATG TGCCAAGCGA TAAGAGCGGC
    AATCTGGCGT GGTACTTCCC TTGTAACACG TTCAACCAAG ATGGTTCATC CAAGCCCAAT
    ACCACGATAC CTTATTACCT GGGCTATGCC TGTCACACGA CCGCTAACGC CAGGGACTCT
    TTCTATCTCG GCCTCAAGAG CTCCGCCGAT GTCTACTTTA CGTGGGATGA CATCAAGAAC
    AGCTCTCGAA ACCTTGTTGT GTACTCTGGC CATGTTCTCG ACCTTGATCT GCTTCACTGG
    TTCAACGACA CTCAAGTCAC GTATCCCGCC CGCTTCAAGG AGCTGAGGGA TAAGAATACT
    GCTGGAAACC AGGCTATTCG TGGACGCGAT ATTACCCATG CTTTCCAGTC GAGCAAAGAC
    AAGCAGATTG CCGAATGCTT TGAGGAGATC ATCAAGGTCG GTTCGGTGGA TACCGAGACA
    GTTGGCTGCA TTGCGTCAAA GGTCGTTCTC TATGTGTCCC TCGTCCTTAT CCTCGCGGTC
    GTCCTCGCCA GATTCGTCTT GGCTCTAATC TTCCAATGGT TCATCAGCAA GACGTATGCG
    GCTGCTAAGA CCTCCCAAAC GTCTGACCAG CGAAAGCGAA ACCGTCAGAT TGAAGACTGG
    ACTGAAGACA TTTACAGAGC TCCCCCAAGG CTACCAGGGG AGGTCGGTAG CAGTGTCGCT
    GGAAGCTCCG ATCGTCAAAG CAAGCGCAGC AGTGCTTTCT TGCCCACACA CTCTCGTTTC
    TCCACCGTCT ACGGCAATGA GCGCGGCAAT AGGAAGCCTG GACTTCCTAC AACCATGGCC
    AGCCAGAATG CCGCTGGTCA ACTACTCCAT CCTGGAACCA TATACGGCCA AGGAAATGAG
    AGTCGGTCAA GCTTCCTCAA ATCTGATGCT TACGGATCAA GCAGCAGTCC AGCAGACGGC
    CCAGGTCCTG CCGGATTTAT TCATGAAGCT GTCGTTCCTC AGCCGCCTTC CGACTGGATG
    CCGTTTGGTT TCCCCCTCGC TCACACCATC TGCCTTGTCA CAGCCTACTC CGAGGGTGAG
    ATGGGCGTTC GTACCACTCT GGACTCGATT GCAATGACGG ACTACCCCAA CAGCCACAAG
    GTTATCCTCG TTATTTGCGA TGGTATTATC AAGGGCCACG GAGAGGAACA CTCAACCCCC
    GATATCATTC TCGGCATGAT GAAAGACCAC ACGATTCACC CCGATGACGT TGAGCCATTC
    TCTTATGTTG CGGTTGCAAC TGGTTCTAAG CGCCACAACA TGGCCAAGGT CTACACCGGC
    TTCTACGATT ATGGCACTAA CTCTGCCATT CCTCTGGAGA AGCAGCAGCG TGTTCCTATG
    ATGATGGTTG TGAAGTGCGG CACTCCTGCT GAAGCCTCCA AGTCGAAGCC TGGAAACCGT
    GGCAAGCGTG ACAGTCAGAT CATCCTTATG TCTTTTCTCC AGAAGGTTAT GTTCGACGAG
    CGTATGACGG AGCTAGAATA CGAGATGTTC AATGGCTTGT GGAAGATCAC GGGCATTTCT
    CCTGACTTCT ATGAGATCGT GCTCATGGTT GACGCTGATA CCAAGGTCTT CCCCGACAGT
    CTCACGCATA TGATCTCGGC CATGGTCAAG GATCCTGAAA TTATGGGTCT TTGTGGCGAG
    ACCAAGATCG CCAACAAGCG AGCAAGCTGG GTTTCCGCCA TCCAGGTATT CGAGTATTTC
    GTCTCTCATC ATCTTGCCAA GGCTTTCGAG TCCGTCTTTG GCGGTGTCAC CTGTCTTCCC
    GGATGTTTCT GCATGTACCG CATCAAGGCA CCCAAGGGTG CCCAGAACTA CTGGGTCCCT
    ATCTTGGCCA ACCCGGACGT TGTCGAACAC TACTCCGAGA ACGTTGTCGA TACGCTGCAC
    AAGAAGAACC TCTTGCTCCT TGGTGAGGAT CGTTATCTCA GTACGCTCAT GTTGCGCACG
    TTCCCCAAGC GTAAACAAGT ATTTGTGCCT CAGGCCGTTT GCAAGACGAC TGTCCCCGAT
    GAATTCATGG TGTTGCTTTC GCAGCGCCGC AGATGGATCA ACTCGACCAT TCACAATCTG
    ATGGAGCTCG TTCTTGTGCG CGATCTTTGC GGTACCTTCT GTTTCAGTAT GCAGTTCATT
    GTCGGTATCG AGCTGATTGG TACCCTTGTG TTGCCTGCCG CCATTGCCTT TACTTTCTAT
    GTTGTCATCA TCTCAATCAT CAACTCCCCG CCACAAATCA TTCCTCTTGT TCTCCTTGGT
    CTCATCCTCG GTCTCCCAGC CATCCTCGTC GTCGTCACAG CTCACAGCTG GTCCTACATC
    ATCTGGATGT TTATCTACTT GCTCTCCCTG CCCGTGTGGA ACTTTGTTCT GCCTACCTAC
    GCTTTCTGGA AGTTTGACGA TTTCTCATGG GGCGACACGC GCAAGACGGC CGGCGAGAAA
    TCAAGCAAGG GTGGCCACGG AGAAGCCGAG GGAGAGTTTG ATAGCAGCAT GATTACCATG
    AAGCGCTGGG CCGAGTTTGA GCGCGACAGG CGCGTCCGCA GCACGTACTG GGCCGGCTCG
    AGGGACAATG TCATATCCGG GGTAGGGGGT AGTAATGGGT GGGGAAGCAG CCAGCCGAGA
    GGCCATGAGC AGGGGAGACA CTTTGATGAC TACTTTTCGG ACGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011393274.1
    CDS334..4041
    Translation

    Target ORF information:

    RefSeq Version XM_011394972.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A chitin synthase 4, variant (chs-4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011394972.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCTTC CTCGGAGGCC GGGGCCGTCG CCTCCGCACG AGGAGCCACG CCGCTACCGG 
    CAAAGCGGTT CGCGCCGCAG TCGACCACCA CCCGCCGATG TTGAGACCGG ATATGCTCCC
    ATGGCAGGAG AAAGACCAAG CCAGCAGCAA CGCGTACCAT CGATATCTTC ATTTCCGGAA
    ACCCTCCCGT CCCCCAACCC CAATGTCGAA CGAGACCCTT TGGCGCCCAC CCCCGAGCCC
    GCACATCCAT CCGGCATACC TCAGCGGAAG AGGAGTTTGA TTCGACCGGA GCGCAACAGG
    ATTGGCAAAG ACCACCCCAA CTATCACTAC AGAAAACATG CGGCCAACAT GAACACCCTA
    CCGTCCTCAA CCGGACACGA CCCGATATAC GAGGATCTGG AGGGAGCAAC GGACGATGTA
    TCGGGAACTG GCTCGAGGAA CGACGACGAT GTTTCCGAGG AGTCGCCCCC AAGAAGAAAG
    CACTCGACGA AAATGCAGGT CATCGAAACT GAAAAGTCCG GTGATGAAAG ACGACGTCGG
    CGGAAATCGG ATACCACAAA ACATGGTAAA ATTGTCAAGG CCTCCAAGGG AAAGCGCGAA
    AAGTCCGGAG GACTCCCTAC CCCGAGCTTT TGGAACATTT ACTGCGGATT CGTCACCTTC
    TGGTGCCCTG GCTTTGTCCT CAAGTGCTTC GGCATGCCCG AGATGGCTCA ACAGAGGGCG
    TGGAGAGAGA AGATGGGTCT GATCAGCATT ATTCTGCTCA TCATGGGCTT TGTTGGCTTC
    ATCACTTTCG GCTTCACCCA GGTGGTCTGC GGTAAACCTC CGCTCCGTTT GCGCATCAAT
    GAGGTCGGGT CAGGATACAT GATCTTCCAC GGATCTGCAT ATGACCTCAC CAAATCTCAC
    CATCCTCCGG CCGAGGGTAT CCCGCGCCGT CCGGACGGTT TGGGTGCAAA CGTGATCTAT
    GACTTGCCTC AGCATTACGG TGGACAGGAC GGCAGTTTCT TGTTCCAGAA CGTCAACGGC
    AAGTGCAAGG GTCTCATCAC CAAGCAAGAG AACTCCGATG TGCCAAGCGA TAAGAGCGGC
    AATCTGGCGT GGTACTTCCC TTGTAACACG TTCAACCAAG ATGGTTCATC CAAGCCCAAT
    ACCACGATAC CTTATTACCT GGGCTATGCC TGTCACACGA CCGCTAACGC CAGGGACTCT
    TTCTATCTCG GCCTCAAGAG CTCCGCCGAT GTCTACTTTA CGTGGGATGA CATCAAGAAC
    AGCTCTCGAA ACCTTGTTGT GTACTCTGGC CATGTTCTCG ACCTTGATCT GCTTCACTGG
    TTCAACGACA CTCAAGTCAC GTATCCCGCC CGCTTCAAGG AGCTGAGGGA TAAGAATACT
    GCTGGAAACC AGGCTATTCG TGGACGCGAT ATTACCCATG CTTTCCAGTC GAGCAAAGAC
    AAGCAGATTG CCGAATGCTT TGAGGAGATC ATCAAGGTCG GTTCGGTGGA TACCGAGACA
    GTTGGCTGCA TTGCGTCAAA GGTCGTTCTC TATGTGTCCC TCGTCCTTAT CCTCGCGGTC
    GTCCTCGCCA GATTCGTCTT GGCTCTAATC TTCCAATGGT TCATCAGCAA GACGTATGCG
    GCTGCTAAGA CCTCCCAAAC GTCTGACCAG CGAAAGCGAA ACCGTCAGAT TGAAGACTGG
    ACTGAAGACA TTTACAGAGC TCCCCCAAGG CTACCAGGGG AGGTCGGTAG CAGTGTCGCT
    GGAAGCTCCG ATCGTCAAAG CAAGCGCAGC AGTGCTTTCT TGCCCACACA CTCTCGTTTC
    TCCACCGTCT ACGGCAATGA GCGCGGCAAT AGGAAGCCTG GACTTCCTAC AACCATGGCC
    AGCCAGAATG CCGCTGGTCA ACTACTCCAT CCTGGAACCA TATACGGCCA AGGAAATGAG
    AGTCGGTCAA GCTTCCTCAA ATCTGATGCT TACGGATCAA GCAGCAGTCC AGCAGACGGC
    CCAGGTCCTG CCGGATTTAT TCATGAAGCT GTCGTTCCTC AGCCGCCTTC CGACTGGATG
    CCGTTTGGTT TCCCCCTCGC TCACACCATC TGCCTTGTCA CAGCCTACTC CGAGGGTGAG
    ATGGGCGTTC GTACCACTCT GGACTCGATT GCAATGACGG ACTACCCCAA CAGCCACAAG
    GTTATCCTCG TTATTTGCGA TGGTATTATC AAGGGCCACG GAGAGGAACA CTCAACCCCC
    GATATCATTC TCGGCATGAT GAAAGACCAC ACGATTCACC CCGATGACGT TGAGCCATTC
    TCTTATGTTG CGGTTGCAAC TGGTTCTAAG CGCCACAACA TGGCCAAGGT CTACACCGGC
    TTCTACGATT ATGGCACTAA CTCTGCCATT CCTCTGGAGA AGCAGCAGCG TGTTCCTATG
    ATGATGGTTG TGAAGTGCGG CACTCCTGCT GAAGCCTCCA AGTCGAAGCC TGGAAACCGT
    GGCAAGCGTG ACAGTCAGAT CATCCTTATG TCTTTTCTCC AGAAGGTTAT GTTCGACGAG
    CGTATGACGG AGCTAGAATA CGAGATGTTC AATGGCTTGT GGAAGATCAC GGGCATTTCT
    CCTGACTTCT ATGAGATCGT GCTCATGGTT GACGCTGATA CCAAGGTCTT CCCCGACAGT
    CTCACGCATA TGATCTCGGC CATGGTCAAG GATCCTGAAA TTATGGGTCT TTGTGGCGAG
    ACCAAGATCG CCAACAAGCG AGCAAGCTGG GTTTCCGCCA TCCAGGTATT CGAGTATTTC
    GTCTCTCATC ATCTTGCCAA GGCTTTCGAG TCCGTCTTTG GCGGTGTCAC CTGTCTTCCC
    GGATGTTTCT GCATGTACCG CATCAAGGCA CCCAAGGGTG CCCAGAACTA CTGGGTCCCT
    ATCTTGGCCA ACCCGGACGT TGTCGAACAC TACTCCGAGA ACGTTGTCGA TACGCTGCAC
    AAGAAGAACC TCTTGCTCCT TGGTGAGGAT CGTTATCTCA GTACGCTCAT GTTGCGCACG
    TTCCCCAAGC GTAAACAAGT ATTTGTGCCT CAGGCCGTTT GCAAGACGAC TGTCCCCGAT
    GAATTCATGG TGTTGCTTTC GCAGCGCCGC AGATGGATCA ACTCGACCAT TCACAATCTG
    ATGGAGCTCG TTCTTGTGCG CGATCTTTGC GGTACCTTCT GTTTCAGTAT GCAGTTCATT
    GTCGGTATCG AGCTGATTGG TACCCTTGTG TTGCCTGCCG CCATTGCCTT TACTTTCTAT
    GTTGTCATCA TCTCAATCAT CAACTCCCCG CCACAAATCA TTCCTCTTGT TCTCCTTGGT
    CTCATCCTCG GTCTCCCAGC CATCCTCGTC GTCGTCACAG CTCACAGCTG GTCCTACATC
    ATCTGGATGT TTATCTACTT GCTCTCCCTG CCCGTGTGGA ACTTTGTTCT GCCTACCTAC
    GCTTTCTGGA AGTTTGACGA TTTCTCATGG GGCGACACGC GCAAGACGGC CGGCGAGAAA
    TCAAGCAAGG GTGGCCACGG AGAAGCCGAG GGAGAGTTTG ATAGCAGCAT GATTACCATG
    AAGCGCTGGG CCGAGTTTGA GCGCGACAGG CGCGTCCGCA GCACGTACTG GGCCGGCTCG
    AGGGACAATG TCATATCCGG GGTAGGGGGT AGTAATGGGT GGGGAAGCAG CCAGCCGAGA
    GGCCATGAGC AGGGGAGACA CTTTGATGAC TACTTTTCGG ACGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ONf02489
    Clone ID Related Accession (Same CDS sequence) XM_011394972.1 , XM_011394973.1
    Accession Version XM_011394973.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product chitin synthase 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCTTC CTCGGAGGCC GGGGCCGTCG CCTCCGCACG AGGAGCCACG CCGCTACCGG 
    CAAAGCGGTT CGCGCCGCAG TCGACCACCA CCCGCCGATG TTGAGACCGG ATATGCTCCC
    ATGGCAGGAG AAAGACCAAG CCAGCAGCAA CGCGTACCAT CGATATCTTC ATTTCCGGAA
    ACCCTCCCGT CCCCCAACCC CAATGTCGAA CGAGACCCTT TGGCGCCCAC CCCCGAGCCC
    GCACATCCAT CCGGCATACC TCAGCGGAAG AGGAGTTTGA TTCGACCGGA GCGCAACAGG
    ATTGGCAAAG ACCACCCCAA CTATCACTAC AGAAAACATG CGGCCAACAT GAACACCCTA
    CCGTCCTCAA CCGGACACGA CCCGATATAC GAGGATCTGG AGGGAGCAAC GGACGATGTA
    TCGGGAACTG GCTCGAGGAA CGACGACGAT GTTTCCGAGG AGTCGCCCCC AAGAAGAAAG
    CACTCGACGA AAATGCAGGT CATCGAAACT GAAAAGTCCG GTGATGAAAG ACGACGTCGG
    CGGAAATCGG ATACCACAAA ACATGGTAAA ATTGTCAAGG CCTCCAAGGG AAAGCGCGAA
    AAGTCCGGAG GACTCCCTAC CCCGAGCTTT TGGAACATTT ACTGCGGATT CGTCACCTTC
    TGGTGCCCTG GCTTTGTCCT CAAGTGCTTC GGCATGCCCG AGATGGCTCA ACAGAGGGCG
    TGGAGAGAGA AGATGGGTCT GATCAGCATT ATTCTGCTCA TCATGGGCTT TGTTGGCTTC
    ATCACTTTCG GCTTCACCCA GGTGGTCTGC GGTAAACCTC CGCTCCGTTT GCGCATCAAT
    GAGGTCGGGT CAGGATACAT GATCTTCCAC GGATCTGCAT ATGACCTCAC CAAATCTCAC
    CATCCTCCGG CCGAGGGTAT CCCGCGCCGT CCGGACGGTT TGGGTGCAAA CGTGATCTAT
    GACTTGCCTC AGCATTACGG TGGACAGGAC GGCAGTTTCT TGTTCCAGAA CGTCAACGGC
    AAGTGCAAGG GTCTCATCAC CAAGCAAGAG AACTCCGATG TGCCAAGCGA TAAGAGCGGC
    AATCTGGCGT GGTACTTCCC TTGTAACACG TTCAACCAAG ATGGTTCATC CAAGCCCAAT
    ACCACGATAC CTTATTACCT GGGCTATGCC TGTCACACGA CCGCTAACGC CAGGGACTCT
    TTCTATCTCG GCCTCAAGAG CTCCGCCGAT GTCTACTTTA CGTGGGATGA CATCAAGAAC
    AGCTCTCGAA ACCTTGTTGT GTACTCTGGC CATGTTCTCG ACCTTGATCT GCTTCACTGG
    TTCAACGACA CTCAAGTCAC GTATCCCGCC CGCTTCAAGG AGCTGAGGGA TAAGAATACT
    GCTGGAAACC AGGCTATTCG TGGACGCGAT ATTACCCATG CTTTCCAGTC GAGCAAAGAC
    AAGCAGATTG CCGAATGCTT TGAGGAGATC ATCAAGGTCG GTTCGGTGGA TACCGAGACA
    GTTGGCTGCA TTGCGTCAAA GGTCGTTCTC TATGTGTCCC TCGTCCTTAT CCTCGCGGTC
    GTCCTCGCCA GATTCGTCTT GGCTCTAATC TTCCAATGGT TCATCAGCAA GACGTATGCG
    GCTGCTAAGA CCTCCCAAAC GTCTGACCAG CGAAAGCGAA ACCGTCAGAT TGAAGACTGG
    ACTGAAGACA TTTACAGAGC TCCCCCAAGG CTACCAGGGG AGGTCGGTAG CAGTGTCGCT
    GGAAGCTCCG ATCGTCAAAG CAAGCGCAGC AGTGCTTTCT TGCCCACACA CTCTCGTTTC
    TCCACCGTCT ACGGCAATGA GCGCGGCAAT AGGAAGCCTG GACTTCCTAC AACCATGGCC
    AGCCAGAATG CCGCTGGTCA ACTACTCCAT CCTGGAACCA TATACGGCCA AGGAAATGAG
    AGTCGGTCAA GCTTCCTCAA ATCTGATGCT TACGGATCAA GCAGCAGTCC AGCAGACGGC
    CCAGGTCCTG CCGGATTTAT TCATGAAGCT GTCGTTCCTC AGCCGCCTTC CGACTGGATG
    CCGTTTGGTT TCCCCCTCGC TCACACCATC TGCCTTGTCA CAGCCTACTC CGAGGGTGAG
    ATGGGCGTTC GTACCACTCT GGACTCGATT GCAATGACGG ACTACCCCAA CAGCCACAAG
    GTTATCCTCG TTATTTGCGA TGGTATTATC AAGGGCCACG GAGAGGAACA CTCAACCCCC
    GATATCATTC TCGGCATGAT GAAAGACCAC ACGATTCACC CCGATGACGT TGAGCCATTC
    TCTTATGTTG CGGTTGCAAC TGGTTCTAAG CGCCACAACA TGGCCAAGGT CTACACCGGC
    TTCTACGATT ATGGCACTAA CTCTGCCATT CCTCTGGAGA AGCAGCAGCG TGTTCCTATG
    ATGATGGTTG TGAAGTGCGG CACTCCTGCT GAAGCCTCCA AGTCGAAGCC TGGAAACCGT
    GGCAAGCGTG ACAGTCAGAT CATCCTTATG TCTTTTCTCC AGAAGGTTAT GTTCGACGAG
    CGTATGACGG AGCTAGAATA CGAGATGTTC AATGGCTTGT GGAAGATCAC GGGCATTTCT
    CCTGACTTCT ATGAGATCGT GCTCATGGTT GACGCTGATA CCAAGGTCTT CCCCGACAGT
    CTCACGCATA TGATCTCGGC CATGGTCAAG GATCCTGAAA TTATGGGTCT TTGTGGCGAG
    ACCAAGATCG CCAACAAGCG AGCAAGCTGG GTTTCCGCCA TCCAGGTATT CGAGTATTTC
    GTCTCTCATC ATCTTGCCAA GGCTTTCGAG TCCGTCTTTG GCGGTGTCAC CTGTCTTCCC
    GGATGTTTCT GCATGTACCG CATCAAGGCA CCCAAGGGTG CCCAGAACTA CTGGGTCCCT
    ATCTTGGCCA ACCCGGACGT TGTCGAACAC TACTCCGAGA ACGTTGTCGA TACGCTGCAC
    AAGAAGAACC TCTTGCTCCT TGGTGAGGAT CGTTATCTCA GTACGCTCAT GTTGCGCACG
    TTCCCCAAGC GTAAACAAGT ATTTGTGCCT CAGGCCGTTT GCAAGACGAC TGTCCCCGAT
    GAATTCATGG TGTTGCTTTC GCAGCGCCGC AGATGGATCA ACTCGACCAT TCACAATCTG
    ATGGAGCTCG TTCTTGTGCG CGATCTTTGC GGTACCTTCT GTTTCAGTAT GCAGTTCATT
    GTCGGTATCG AGCTGATTGG TACCCTTGTG TTGCCTGCCG CCATTGCCTT TACTTTCTAT
    GTTGTCATCA TCTCAATCAT CAACTCCCCG CCACAAATCA TTCCTCTTGT TCTCCTTGGT
    CTCATCCTCG GTCTCCCAGC CATCCTCGTC GTCGTCACAG CTCACAGCTG GTCCTACATC
    ATCTGGATGT TTATCTACTT GCTCTCCCTG CCCGTGTGGA ACTTTGTTCT GCCTACCTAC
    GCTTTCTGGA AGTTTGACGA TTTCTCATGG GGCGACACGC GCAAGACGGC CGGCGAGAAA
    TCAAGCAAGG GTGGCCACGG AGAAGCCGAG GGAGAGTTTG ATAGCAGCAT GATTACCATG
    AAGCGCTGGG CCGAGTTTGA GCGCGACAGG CGCGTCCGCA GCACGTACTG GGCCGGCTCG
    AGGGACAATG TCATATCCGG GGTAGGGGGT AGTAATGGGT GGGGAAGCAG CCAGCCGAGA
    GGCCATGAGC AGGGGAGACA CTTTGATGAC TACTTTTCGG ACGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011393275.1
    CDS609..4316
    Translation

    Target ORF information:

    RefSeq Version XM_011394973.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A chitin synthase 4 (chs-4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011394973.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCTCTTC CTCGGAGGCC GGGGCCGTCG CCTCCGCACG AGGAGCCACG CCGCTACCGG 
    CAAAGCGGTT CGCGCCGCAG TCGACCACCA CCCGCCGATG TTGAGACCGG ATATGCTCCC
    ATGGCAGGAG AAAGACCAAG CCAGCAGCAA CGCGTACCAT CGATATCTTC ATTTCCGGAA
    ACCCTCCCGT CCCCCAACCC CAATGTCGAA CGAGACCCTT TGGCGCCCAC CCCCGAGCCC
    GCACATCCAT CCGGCATACC TCAGCGGAAG AGGAGTTTGA TTCGACCGGA GCGCAACAGG
    ATTGGCAAAG ACCACCCCAA CTATCACTAC AGAAAACATG CGGCCAACAT GAACACCCTA
    CCGTCCTCAA CCGGACACGA CCCGATATAC GAGGATCTGG AGGGAGCAAC GGACGATGTA
    TCGGGAACTG GCTCGAGGAA CGACGACGAT GTTTCCGAGG AGTCGCCCCC AAGAAGAAAG
    CACTCGACGA AAATGCAGGT CATCGAAACT GAAAAGTCCG GTGATGAAAG ACGACGTCGG
    CGGAAATCGG ATACCACAAA ACATGGTAAA ATTGTCAAGG CCTCCAAGGG AAAGCGCGAA
    AAGTCCGGAG GACTCCCTAC CCCGAGCTTT TGGAACATTT ACTGCGGATT CGTCACCTTC
    TGGTGCCCTG GCTTTGTCCT CAAGTGCTTC GGCATGCCCG AGATGGCTCA ACAGAGGGCG
    TGGAGAGAGA AGATGGGTCT GATCAGCATT ATTCTGCTCA TCATGGGCTT TGTTGGCTTC
    ATCACTTTCG GCTTCACCCA GGTGGTCTGC GGTAAACCTC CGCTCCGTTT GCGCATCAAT
    GAGGTCGGGT CAGGATACAT GATCTTCCAC GGATCTGCAT ATGACCTCAC CAAATCTCAC
    CATCCTCCGG CCGAGGGTAT CCCGCGCCGT CCGGACGGTT TGGGTGCAAA CGTGATCTAT
    GACTTGCCTC AGCATTACGG TGGACAGGAC GGCAGTTTCT TGTTCCAGAA CGTCAACGGC
    AAGTGCAAGG GTCTCATCAC CAAGCAAGAG AACTCCGATG TGCCAAGCGA TAAGAGCGGC
    AATCTGGCGT GGTACTTCCC TTGTAACACG TTCAACCAAG ATGGTTCATC CAAGCCCAAT
    ACCACGATAC CTTATTACCT GGGCTATGCC TGTCACACGA CCGCTAACGC CAGGGACTCT
    TTCTATCTCG GCCTCAAGAG CTCCGCCGAT GTCTACTTTA CGTGGGATGA CATCAAGAAC
    AGCTCTCGAA ACCTTGTTGT GTACTCTGGC CATGTTCTCG ACCTTGATCT GCTTCACTGG
    TTCAACGACA CTCAAGTCAC GTATCCCGCC CGCTTCAAGG AGCTGAGGGA TAAGAATACT
    GCTGGAAACC AGGCTATTCG TGGACGCGAT ATTACCCATG CTTTCCAGTC GAGCAAAGAC
    AAGCAGATTG CCGAATGCTT TGAGGAGATC ATCAAGGTCG GTTCGGTGGA TACCGAGACA
    GTTGGCTGCA TTGCGTCAAA GGTCGTTCTC TATGTGTCCC TCGTCCTTAT CCTCGCGGTC
    GTCCTCGCCA GATTCGTCTT GGCTCTAATC TTCCAATGGT TCATCAGCAA GACGTATGCG
    GCTGCTAAGA CCTCCCAAAC GTCTGACCAG CGAAAGCGAA ACCGTCAGAT TGAAGACTGG
    ACTGAAGACA TTTACAGAGC TCCCCCAAGG CTACCAGGGG AGGTCGGTAG CAGTGTCGCT
    GGAAGCTCCG ATCGTCAAAG CAAGCGCAGC AGTGCTTTCT TGCCCACACA CTCTCGTTTC
    TCCACCGTCT ACGGCAATGA GCGCGGCAAT AGGAAGCCTG GACTTCCTAC AACCATGGCC
    AGCCAGAATG CCGCTGGTCA ACTACTCCAT CCTGGAACCA TATACGGCCA AGGAAATGAG
    AGTCGGTCAA GCTTCCTCAA ATCTGATGCT TACGGATCAA GCAGCAGTCC AGCAGACGGC
    CCAGGTCCTG CCGGATTTAT TCATGAAGCT GTCGTTCCTC AGCCGCCTTC CGACTGGATG
    CCGTTTGGTT TCCCCCTCGC TCACACCATC TGCCTTGTCA CAGCCTACTC CGAGGGTGAG
    ATGGGCGTTC GTACCACTCT GGACTCGATT GCAATGACGG ACTACCCCAA CAGCCACAAG
    GTTATCCTCG TTATTTGCGA TGGTATTATC AAGGGCCACG GAGAGGAACA CTCAACCCCC
    GATATCATTC TCGGCATGAT GAAAGACCAC ACGATTCACC CCGATGACGT TGAGCCATTC
    TCTTATGTTG CGGTTGCAAC TGGTTCTAAG CGCCACAACA TGGCCAAGGT CTACACCGGC
    TTCTACGATT ATGGCACTAA CTCTGCCATT CCTCTGGAGA AGCAGCAGCG TGTTCCTATG
    ATGATGGTTG TGAAGTGCGG CACTCCTGCT GAAGCCTCCA AGTCGAAGCC TGGAAACCGT
    GGCAAGCGTG ACAGTCAGAT CATCCTTATG TCTTTTCTCC AGAAGGTTAT GTTCGACGAG
    CGTATGACGG AGCTAGAATA CGAGATGTTC AATGGCTTGT GGAAGATCAC GGGCATTTCT
    CCTGACTTCT ATGAGATCGT GCTCATGGTT GACGCTGATA CCAAGGTCTT CCCCGACAGT
    CTCACGCATA TGATCTCGGC CATGGTCAAG GATCCTGAAA TTATGGGTCT TTGTGGCGAG
    ACCAAGATCG CCAACAAGCG AGCAAGCTGG GTTTCCGCCA TCCAGGTATT CGAGTATTTC
    GTCTCTCATC ATCTTGCCAA GGCTTTCGAG TCCGTCTTTG GCGGTGTCAC CTGTCTTCCC
    GGATGTTTCT GCATGTACCG CATCAAGGCA CCCAAGGGTG CCCAGAACTA CTGGGTCCCT
    ATCTTGGCCA ACCCGGACGT TGTCGAACAC TACTCCGAGA ACGTTGTCGA TACGCTGCAC
    AAGAAGAACC TCTTGCTCCT TGGTGAGGAT CGTTATCTCA GTACGCTCAT GTTGCGCACG
    TTCCCCAAGC GTAAACAAGT ATTTGTGCCT CAGGCCGTTT GCAAGACGAC TGTCCCCGAT
    GAATTCATGG TGTTGCTTTC GCAGCGCCGC AGATGGATCA ACTCGACCAT TCACAATCTG
    ATGGAGCTCG TTCTTGTGCG CGATCTTTGC GGTACCTTCT GTTTCAGTAT GCAGTTCATT
    GTCGGTATCG AGCTGATTGG TACCCTTGTG TTGCCTGCCG CCATTGCCTT TACTTTCTAT
    GTTGTCATCA TCTCAATCAT CAACTCCCCG CCACAAATCA TTCCTCTTGT TCTCCTTGGT
    CTCATCCTCG GTCTCCCAGC CATCCTCGTC GTCGTCACAG CTCACAGCTG GTCCTACATC
    ATCTGGATGT TTATCTACTT GCTCTCCCTG CCCGTGTGGA ACTTTGTTCT GCCTACCTAC
    GCTTTCTGGA AGTTTGACGA TTTCTCATGG GGCGACACGC GCAAGACGGC CGGCGAGAAA
    TCAAGCAAGG GTGGCCACGG AGAAGCCGAG GGAGAGTTTG ATAGCAGCAT GATTACCATG
    AAGCGCTGGG CCGAGTTTGA GCGCGACAGG CGCGTCCGCA GCACGTACTG GGCCGGCTCG
    AGGGACAATG TCATATCCGG GGTAGGGGGT AGTAATGGGT GGGGAAGCAG CCAGCCGAGA
    GGCCATGAGC AGGGGAGACA CTTTGATGAC TACTTTTCGG ACGCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
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