NCU04800 cDNA ORF clone, Neurospora crassa OR74A

The following NCU04800 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU04800 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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ONf11403 XM_955333.3
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Neurospora crassa OR74A E3 ubiquitin-protein ligase (NCU04800), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf11403
    Clone ID Related Accession (Same CDS sequence) XM_955333.3
    Accession Version XM_955333.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3438bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product E3 ubiquitin-protein ligase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026506). On Feb 23, 2015 this sequence version replaced XM_955333.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTTCCCA CCTTTACTGG TAGCTCTAGG AAAGGTCGAA ATGTCAACCT CAGCGGTCAA 
    CGGACAAGCA ACCCCTTCAC CAGCACCTCA TGGGGCGCCG GTTCAGCGAC GGGAGCTTCC
    AAAACAGTAG CGCATGCCCA AGCAGAACGT CAGCAAAGGC AACAGGAACG AGACCGGCTC
    AGAGCGGCGC AACGAATACA ACGAACATGG AGGGGACATC GTGTGCGACG AACCCTACGA
    GATGAACAAC GCCAGGCTAT CGACCGTCTG TACGAAGCGC AACCCGTTGA TGCTGGGCAG
    AGGTCAGCGC AGGCATTACC TCTGGTGTTT GCCGCCTTCC AAGCTTTCAA CGTACAAGAC
    CGTCACAGGC TAGCTTTGGT TACCGAAGAT CTTCTCCAGA CAGACTTCTC TGCCTTTAGC
    TTGGTATTCT TGGGGTGGGA CAAACTGGGC AAACTAAGGC GGATTTTACT TGCTGCTCTG
    ACAAGGTGTG ATCTCGGAAA CGAGCAGACT CAGGTGGCTC TTTTCCTACG GGCCCTTGAT
    GCGATCAGGA CCAAGACGTC GGACTTCGAG ACGTTTGGAC AAGTTCTGGG AGAGTGGTAC
    AAGGTACTCG GAAGGTTATG CCAGGAGACT GGTTTGCCCT CTTCTGTCCA AGAGCTCATT
    TGCAGTCTGG TGGTTGCACC GCTTGATGTA CCCCACGATT TAGACTTCAA ACATACAGCC
    TACCAAAACT TGGTTGTTTC TTTTCTGACA CAGCCCGGCC CGGCGCGATG CTCGGACCTG
    ATTACGAGAC TAGCCGCCAA CATAGACCTA GATCGGCTGT CAAATTATAT CATTGACCTC
    TCGCATAAGC TTTCAAAGCA TGACCCGAAC GCTGTCCTCT GGCTTATCGC ACACTACATT
    GCGATCAATG GGGCGAAGGA ACAACGAAGC TCAAGGCTTC TTTTCATCAA GACCCTAGAA
    TCACTCTTCC CTCTGTGCTC AGCCCAGATA AGGGCGGGTT TTGCCGTTGC TCGACCAAAG
    GACACGGGTT ACTTTGCGGA AGCCGAGCAA GATGGATCCG ACGTTCTGCA TCCCTTTGTG
    CGACAACAGC TTCAGTCGCT AGTTGATAAA GGAGCCATCC AAGACCTTCT AAGCGCTTTT
    ACCGCAACTA CCGGGGAAAC CTCTACTTCG GCGGATGACG ACGCCAGTTA CCTCGCTGGT
    TTCATCCTGA CTTTGATCAA CTGCTTTCCT GCTTTTAGCG AGGACATTCG CATGTTCCTC
    TATGTGGCTG ACATCTCCAC TGCCCAAGGC CGTATTCCAA CGGCCAAGTA CTTCTGGAGT
    GCTACACAAA AGACGTCCGT GTTCTCAGAG ATTGCGTCCA GCCAGGACTC CACGCTAGAC
    TTTCTTCGAC GCAAACCGTC ATCACCAGCT GCCGAGGCGG TGTGGCATCG AGAATGGAAA
    ACTGTTCTTC TGTTCCTTGA ATCGTACATC TTCGTTTTGC GGCTCATGGA CGACGATGAT
    TTCTTCAGCA CTTTAAACCC CCATCCTAAC TTCGGCGGCC CTTCCTCTCG ACTACGTCTG
    GCGGGAATGT CGCTGAGCGA ACTGAAGCGC TTGACACAAT TTCTCAAGCA CCTCAGTTTC
    ACAATGTACT ACAATGCTAA GGAGCTACTG ACAAACAGCC CTTGGGCCGA GACGCCCAAC
    TCGAATCGCC CTAGAGGCGA TGGCGGTCAA GCTTCCAAAA ATGATAGCTT CCTTCCTCCT
    GCCGGAGTTG ATTTCACTAC GCTCAGAGAC TTAGCAACGA CAGCCATGAA GATGCTTTAC
    GAACGGGACT CGAGACGACC ATTTTTGCCC GAAGGCCACT GGCTGATGAC ATCCAAGTTC
    GACATGGAAG GGTTCCTTGC GGCTGTGGTT CTTGAAGAGC AGAGACAACA CGAGCTGAGC
    AATACCGAAG ATGGTGCCGA CAGCTCCGAG GATGATCAAC AGGAATGGTC TCACTACGCA
    CATCTTGAGA GGCTCAGGCA GAAGAATAGG AAGGCCGCCC GCAACCACTT ATTGTCTGCA
    ATCACCCCGA AGCTTGAGAT TCTGCGCAAT ATGCCTTTTG CTATCCCATT TGAGACGCGC
    GTTCAAATCT TCCGACAGTT CATCCAACTG GATAAGCAGC GCAGACGTGG AGGAGATGGC
    GATCCAGACA TGTGGAGAGC TCATATGCTA CGCAATGGCT TTGGTGCCCA GCACCCCCTG
    GGTAAACACA CGGCTCGGAT CCGAAGAGGT CGAGTTTTTG AGGATGCCAT GAAACAGCTT
    TGGGAGTTGG GCGAAGGACT CAAAGAGCCG ATCCAGGTTA CGTTTGAGGA CGAATTTGGA
    ATGCAGGAGG CGGGTATCGA TGGCGGCGGC GTCACCAAAG AGTTCCTTGA CAGCGTAACT
    ACAGAGGCAT TTACCCACAC CGAACTCTTT GTCACGAACA GCAAGAACGC ATACTACCCA
    AATCCCACCC TTATCGATCG GATCAGGAAC ATGCGGGTAG GAGAACCGGA AGAAACACAG
    CGTGCTGTAG AACAGGTGCT CAAAATGTAC GAATTCCTGG GGCGAATTAT CGGCAAATGC
    ATGTACGAGG GGATCCTGAT CGACATCGTC TTCGCCGGCT TCTTCCTCCT CAAATGGGCC
    ACCGCCAACG ACACCCCAGG CGGCGGTAGC AACCCCCTAG CATCATCCTC CTCCACCACC
    GCCGGCTACC GCGCCAACAT CAACGACCTG CGCGAACTCG ACGAGGAGCT CTACCAAGGC
    ATGCTAAGCC TCAAAAACTA CACGGGCGAC GTGCAAACCG ACCTAGCTCT CGACTTCACC
    ATTACCGACC CCATCAACTC ACCCGGCGAA CCCACCCGCA CCATCATCCG CCCGCTAATC
    CCCCACGGCG ACTCGACGCC CGTCACCAAC GAGAACCGCC CCCTCTACAT CTCCTACGTT
    GCCCGCCACC GCCTCGTCCG CCAACCCTAC CCGCAAACGC GCGCTTTCCT GCGCGGTTTA
    GGCTCCGTCA TCGACCCCAG CTGGCTCTCC ATGTTCAACC AACTCGAGCT CCAACGCCTC
    GTCGGCGGCG ACAGCTCCGA AATCGACGTT GAAGATCTCC GTCGCAACAC GCACTACAAC
    GGGGTGTACC AGATCGGTCT TGATGGGGAG GAGCACCCCA CCATTCAGAT CTTTTGGGAG
    GTGATGCATG AGCTGAAGGA CGAGGAGAGG AGGGAGGTCC TGAAATATGT GACGTCAACG
    CCGAGAGCGC CGCTGTTGGG GTTTGGTCAG TTGAGGCCCT ATTTCACGAT CAGGGATGCC
    GGGCAGGATG AGGACCGGTT GCCGAGTGCG AGTACGTGTG TGAATTTGCT CAAGCTGCCG
    CAGTATCGGA GTAAGGGGAG ACTGAAGGAG AAACTGCTGT ATGCGGTGAA GAGTGGGGCT
    GGGTTTAATT TGAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_960426.3
    CDS274..3711
    Translation

    Target ORF information:

    RefSeq Version XM_955333.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A E3 ubiquitin-protein ligase (NCU04800), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_955333.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTTTCCCA CCTTTACTGG TAGCTCTAGG AAAGGTCGAA ATGTCAACCT CAGCGGTCAA 
    CGGACAAGCA ACCCCTTCAC CAGCACCTCA TGGGGCGCCG GTTCAGCGAC GGGAGCTTCC
    AAAACAGTAG CGCATGCCCA AGCAGAACGT CAGCAAAGGC AACAGGAACG AGACCGGCTC
    AGAGCGGCGC AACGAATACA ACGAACATGG AGGGGACATC GTGTGCGACG AACCCTACGA
    GATGAACAAC GCCAGGCTAT CGACCGTCTG TACGAAGCGC AACCCGTTGA TGCTGGGCAG
    AGGTCAGCGC AGGCATTACC TCTGGTGTTT GCCGCCTTCC AAGCTTTCAA CGTACAAGAC
    CGTCACAGGC TAGCTTTGGT TACCGAAGAT CTTCTCCAGA CAGACTTCTC TGCCTTTAGC
    TTGGTATTCT TGGGGTGGGA CAAACTGGGC AAACTAAGGC GGATTTTACT TGCTGCTCTG
    ACAAGGTGTG ATCTCGGAAA CGAGCAGACT CAGGTGGCTC TTTTCCTACG GGCCCTTGAT
    GCGATCAGGA CCAAGACGTC GGACTTCGAG ACGTTTGGAC AAGTTCTGGG AGAGTGGTAC
    AAGGTACTCG GAAGGTTATG CCAGGAGACT GGTTTGCCCT CTTCTGTCCA AGAGCTCATT
    TGCAGTCTGG TGGTTGCACC GCTTGATGTA CCCCACGATT TAGACTTCAA ACATACAGCC
    TACCAAAACT TGGTTGTTTC TTTTCTGACA CAGCCCGGCC CGGCGCGATG CTCGGACCTG
    ATTACGAGAC TAGCCGCCAA CATAGACCTA GATCGGCTGT CAAATTATAT CATTGACCTC
    TCGCATAAGC TTTCAAAGCA TGACCCGAAC GCTGTCCTCT GGCTTATCGC ACACTACATT
    GCGATCAATG GGGCGAAGGA ACAACGAAGC TCAAGGCTTC TTTTCATCAA GACCCTAGAA
    TCACTCTTCC CTCTGTGCTC AGCCCAGATA AGGGCGGGTT TTGCCGTTGC TCGACCAAAG
    GACACGGGTT ACTTTGCGGA AGCCGAGCAA GATGGATCCG ACGTTCTGCA TCCCTTTGTG
    CGACAACAGC TTCAGTCGCT AGTTGATAAA GGAGCCATCC AAGACCTTCT AAGCGCTTTT
    ACCGCAACTA CCGGGGAAAC CTCTACTTCG GCGGATGACG ACGCCAGTTA CCTCGCTGGT
    TTCATCCTGA CTTTGATCAA CTGCTTTCCT GCTTTTAGCG AGGACATTCG CATGTTCCTC
    TATGTGGCTG ACATCTCCAC TGCCCAAGGC CGTATTCCAA CGGCCAAGTA CTTCTGGAGT
    GCTACACAAA AGACGTCCGT GTTCTCAGAG ATTGCGTCCA GCCAGGACTC CACGCTAGAC
    TTTCTTCGAC GCAAACCGTC ATCACCAGCT GCCGAGGCGG TGTGGCATCG AGAATGGAAA
    ACTGTTCTTC TGTTCCTTGA ATCGTACATC TTCGTTTTGC GGCTCATGGA CGACGATGAT
    TTCTTCAGCA CTTTAAACCC CCATCCTAAC TTCGGCGGCC CTTCCTCTCG ACTACGTCTG
    GCGGGAATGT CGCTGAGCGA ACTGAAGCGC TTGACACAAT TTCTCAAGCA CCTCAGTTTC
    ACAATGTACT ACAATGCTAA GGAGCTACTG ACAAACAGCC CTTGGGCCGA GACGCCCAAC
    TCGAATCGCC CTAGAGGCGA TGGCGGTCAA GCTTCCAAAA ATGATAGCTT CCTTCCTCCT
    GCCGGAGTTG ATTTCACTAC GCTCAGAGAC TTAGCAACGA CAGCCATGAA GATGCTTTAC
    GAACGGGACT CGAGACGACC ATTTTTGCCC GAAGGCCACT GGCTGATGAC ATCCAAGTTC
    GACATGGAAG GGTTCCTTGC GGCTGTGGTT CTTGAAGAGC AGAGACAACA CGAGCTGAGC
    AATACCGAAG ATGGTGCCGA CAGCTCCGAG GATGATCAAC AGGAATGGTC TCACTACGCA
    CATCTTGAGA GGCTCAGGCA GAAGAATAGG AAGGCCGCCC GCAACCACTT ATTGTCTGCA
    ATCACCCCGA AGCTTGAGAT TCTGCGCAAT ATGCCTTTTG CTATCCCATT TGAGACGCGC
    GTTCAAATCT TCCGACAGTT CATCCAACTG GATAAGCAGC GCAGACGTGG AGGAGATGGC
    GATCCAGACA TGTGGAGAGC TCATATGCTA CGCAATGGCT TTGGTGCCCA GCACCCCCTG
    GGTAAACACA CGGCTCGGAT CCGAAGAGGT CGAGTTTTTG AGGATGCCAT GAAACAGCTT
    TGGGAGTTGG GCGAAGGACT CAAAGAGCCG ATCCAGGTTA CGTTTGAGGA CGAATTTGGA
    ATGCAGGAGG CGGGTATCGA TGGCGGCGGC GTCACCAAAG AGTTCCTTGA CAGCGTAACT
    ACAGAGGCAT TTACCCACAC CGAACTCTTT GTCACGAACA GCAAGAACGC ATACTACCCA
    AATCCCACCC TTATCGATCG GATCAGGAAC ATGCGGGTAG GAGAACCGGA AGAAACACAG
    CGTGCTGTAG AACAGGTGCT CAAAATGTAC GAATTCCTGG GGCGAATTAT CGGCAAATGC
    ATGTACGAGG GGATCCTGAT CGACATCGTC TTCGCCGGCT TCTTCCTCCT CAAATGGGCC
    ACCGCCAACG ACACCCCAGG CGGCGGTAGC AACCCCCTAG CATCATCCTC CTCCACCACC
    GCCGGCTACC GCGCCAACAT CAACGACCTG CGCGAACTCG ACGAGGAGCT CTACCAAGGC
    ATGCTAAGCC TCAAAAACTA CACGGGCGAC GTGCAAACCG ACCTAGCTCT CGACTTCACC
    ATTACCGACC CCATCAACTC ACCCGGCGAA CCCACCCGCA CCATCATCCG CCCGCTAATC
    CCCCACGGCG ACTCGACGCC CGTCACCAAC GAGAACCGCC CCCTCTACAT CTCCTACGTT
    GCCCGCCACC GCCTCGTCCG CCAACCCTAC CCGCAAACGC GCGCTTTCCT GCGCGGTTTA
    GGCTCCGTCA TCGACCCCAG CTGGCTCTCC ATGTTCAACC AACTCGAGCT CCAACGCCTC
    GTCGGCGGCG ACAGCTCCGA AATCGACGTT GAAGATCTCC GTCGCAACAC GCACTACAAC
    GGGGTGTACC AGATCGGTCT TGATGGGGAG GAGCACCCCA CCATTCAGAT CTTTTGGGAG
    GTGATGCATG AGCTGAAGGA CGAGGAGAGG AGGGAGGTCC TGAAATATGT GACGTCAACG
    CCGAGAGCGC CGCTGTTGGG GTTTGGTCAG TTGAGGCCCT ATTTCACGAT CAGGGATGCC
    GGGCAGGATG AGGACCGGTT GCCGAGTGCG AGTACGTGTG TGAATTTGCT CAAGCTGCCG
    CAGTATCGGA GTAAGGGGAG ACTGAAGGAG AAACTGCTGT ATGCGGTGAA GAGTGGGGCT
    GGGTTTAATT TGAGTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B