mik-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	mik-1
Entrez Gene ID	3875794
Full Name	MEK kinase-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011396809.1	XP_011395111.1	MAP kinase kinase kinase
XM_011396810.1	XP_011395112.1	MAP kinase kinase kinase, variant

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_00883	XP_003718085.1
Neurospora crassa	NCU02234	XP_959647.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mik-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mik-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf07398	XM_011396809.1 Latest version!	Neurospora crassa OR74A MAP kinase kinase kinase (mik-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ONf11129	XM_011396810.1 Latest version!	Neurospora crassa OR74A MAP kinase kinase kinase, variant (mik-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mik-1 ( XM_011396809.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011395111.1 Neurospora crassa OR74A MAP kinase kinase kinase (mik-1), partial mRNA.

Price & Availability↑

CloneID	ONf07398
Clone ID Related Accession (Same CDS sequence)	XM_011396809.1
Accession Version	XM_011396809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	MAP kinase kinase kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507). COMPLETENESS: incomplete on the 5' end.

ATGTACCAAA ATGGCCAGAG GGACCCTCCG CGGCCCTTTG CGGTCCCGCC GCCGCCGCCG 
CCCATGTCAC CCCCGCAAGT CGGTTCTGGA GTTGCAGGCA TCAACAGCGT CATGAACATT 
CCTCCTCCAC CCCCTCGCTA CCCGGGCGCA CCAGGAACTG TGGGCGGATC CTTATTGCCG 
CCTCCTCCCG GCCCGCCTCC TGGGAACCCG GGTTTCCCTC CGAGCGCAAT AGCCCCGCCG 
AGCGCCTTGG GCGCTGCACC CTGGCATGGC GCCTGGGGAC GCCCATACAA CGGACAAACC 
GCCTTCAATC TTCCTCCTCC TCCGCCTGGT GGAAATGGCG GGCTACAGGC CTACAATCCC 
TTGTTACATG CGCAGGCGGC TGCAGCGGCG GCAGCGGCGG CTGCGTCTGC TTCCAATACC 
CCAGGTCCCC CCGCTACCTC GATCACGATA CCTCCCCCGC CACCGCCAAG CGAGCAAATG 
AGCGCGACCT ATATTCCATC GGCCGGCGAC ACGTATGGTG AAGGTGTGGG CATTCCTGGC 
TTGGGACTAC CTGAGGAGTT GGGTGGCCCA GGGAACTGGA CCGGGGCCGA ACAGCAGCGC 
TTGGGAGGAA CATCTATGGA GGATGCGCAT AGGTTGCACG CTGCTCCCAC GGCGGACCGG 
GGCTTATCGA CCACCAGCAA CACCCCCAAC AGCTCGTCGG CTATTCCGCC CGAGTTGGCA 
GCGCAGTGGC CGCTTGATAG GGTCCTGATG TGGCTTCAGG CAAACAGCTT TTCCCAGGAC 
TGGGTGAATA CGTTCAAGGT CTTGAACCTT CATGGTGCGC AATTTCTGGA GCTCGGCAGT 
GGTCATGGTG GTCGCGGAAA CTTTGGTATG ATGCATCAGC AAGTATATCC GCAGCTGGCG 
CATGAGTGCC AGATGAGCAA GACGGGCTGG GACCAGACGA AAGAGCGGGA GGAGGGCAAG 
CGGATGCGAC GAATGATCAG GGGGATCGTG ACGGGTCGAT CTGTAGATCC ATCCAAAGTT 
GCCGCAAGTG GAGGCGCGCA AGGAGGAGCA GGAGGAGCAG GAGGAGGAGG CCATGCCCGC 
AGAGAGTCCG TCAGTGCAAG CGTCCCGCCT AGTGCAGGTC CCGATTCTGC AGATTCTCCA 
AACGTGGAGT TCAGCAAATC TCATCTCTTT GTGCAGGAAG AGCAAGTCCA GTCCGGACCC 
AAGGCCCCGG GCCCGGGCTT CGCAGGCAGG AGATTCTCCC AATCGCGCGC AACGACGATG 
CCGCTGCTGA CAAGTACCAT GTCATCAGGT GAGCCAAATC ACCGTAGCAT GATGAGGAAT 
CTGGATATAG ACAGCGCCCG TCGTCACAGC CCGAGCACCA GTAACGTGAG CGAATCGGGC 
GACCTAAACG CAGGTACCTT CCGTACGGTC GGGAGAGAAA GGCAGGATAG TCCGATCGGT 
GGCAGTCCTA ACCCTTCTTC TGCCCTCTTC CCTTCTAACG CTGGCAACAC GATCTTGTCA 
TCGTCACCAC ATCGCAGCGG CAGCAGGTTT GGTCATCGTT CGCGCAACAG CAGTGATTCA 
GTATCCTCAA ACGCAGCACA GTACGGGTCT GGTATTCCTG CCGATGCCGC AGCCATGTTC 
AAGAACGGAA GCCTCGCCGA TATGATTAAG AATAGCTTTA ACTCAAACAA CCCTGATCGT 
CGAAACGGTC AAGATGGTGG TCGGCCCTTG GGTGTCGTTG AAACAGGAGA CAGGAGTGCC 
GGGACTGACC CTCCGAACAG CGCCAAGGGC CCAAAATCGT TCTTGTCCTT CTTGTCGCGA 
AACAAGAGAA AGGAAGATGG AACATCACCC GACGAGCTGG ATTCCCCCAC CAGTCCAGCT 
ACCGGCTTCA AGGCTCACTC GCTTGGCAGT CGTGCCGGTC ATGTCAGCGA AACCTCGCTC 
GATCAGCGCC CCGGTTCAAG TATCTCGACT CACGAGCACA GTGTCGGGTT CCAGAGCGGC 
CATAGTAGGA AGAAGAGCGT GGTATCTACT CGGGTTTATC TCCTCGCCAC TCTGGATCAC 
TGGAATTATC GCATGTTGGA TGTTACTGAT ATCGACTCCG CTCATGACCT GAGACAGCTA 
GTGTGCATCA ACCTGGGCCT ACCTGACAGT GAAGGCGCGG CAATCTACGT GACTGAGCTG 
GGCAAGTTCG ACCATGAGGA GCCCTTGGAT GATATGAAAC TACTCACGAA CAAAAGGCTA 
CGGGCGGACG CAATCGGTAC ACTAAAACTT TTTGTAAAAC CGGGCGGTTT GACGAGTTCG 
CCATACCCAG GTCCCGGAAC CCAAAGCGGT CAGCCATCAT CGTATGTTCC TCGCGACGCC 
CCTCCCATGG ACGAGGAAAC TTTCAACAGG CTAAACGGAC AAAGGCAGCG TTCTAGTTCA 
TCGCCGCCGA CGTCAAGGCA GAATACGATA TCCAGCAAGG ACCGGGACGA GAAGATGCCA 
GTGCCGGAGC CAGTCGAGCA CAAGGCAGAG CCCCCGAGAA AGCACGCAAT CAAGGAGACA 
AGCCCGATCG GAGAGACGAC ACCGTTCGGT ATTGTCGGCA GGAAAGTCGA CTTTGACCAG 
CCCCGACTCT CACCCTTCGA GGACAAGAGG CCCGATCACC TGTTTCCGGT ACGAAAGGCA 
CCTGCACCAC CAGGGGACCC ATCGGCTACT TTGAAAAAGG TCAATTCACT GAGTAAGAAA 
ACTGGACAGG GATTCCGACC TAGCACGGCG CATGCCACCC AGCACCATCA TCATAACGCC 
ATAGATCATG GATACCACCA GAGACGAGTA TCGACGGATA TACGCGAGAA CATGACGTCC 
GAAAGGCAGA CGAGGAGGCC AGCCGCCATA AGAGGAACCG CCGAAGTGCC ATCACCATCA 
GGAGGAATCG GCAGTTTGCT CATCAATATA GGGGGCCACC TGGGTGGCAT CGGTCATCCT 
GTCGCTGGTG GTGCTCGCGC ATTATCACCA AACCGTGTTG CCTCAGCGCC CGTCGGGCAT 
GGTGGTGAGA TGAGCGAACA GCAAAGAGCA CGCGCGCCGT CACCATCACT TAGTCCCAAT 
GCTCGTCGGA GACCAAGCGA GTCTCATATC AGCCAGCAAC AATATCAGCA GAATCACCGA 
CCACCATCAT CGCAGCAGCA TCATCCACAT CTCCAGCACC AGGCACAGCC ACAGCCACAG 
CCACAGCCAC AGCAGCAGCA ACAACAGCAA CAGCAACAAC AACAACAGCA ACAATACCAG 
CCGAGACAAC AGCAGACACA GCAACCACAG CAGCAACAAC AAGCACAGCA ACCACAGCAG 
CAACAACAAG CTCCGGCTCA ACACGAGGAG TCCAAGCGCA AGTCCCATGG TCCAGATGTG 
GATTTCAGCG ATAACGATGT GCGCTTCACC CCACCTCCTG AGAGTGAATC CCTCAACGCC 
AGTTCAAACC ATCAAGACTA CGCAGACGAT CAAGATGATT CGGACGACTC TGATGATGGC 
CTCTTCGCGG TTCCGATCTC GAAAAACAAG ACAGCGGAGA CGTCGAAGAC CAAAGATAAT 
TCGGGCGGGA GCGATGAAGG ACTCGGCAAG CGCCCAAGCT TGAAGGTGGC GACCGATCGC 
TCCAAGAAGA ACTTGTCCGT GGCATTCACT ACTCCTCAGG CCAGCCCCAG AATGGCCTCG 
TTCACGGACG AAACTGAGCA TGCTAGTGTG CCAGCTTCCT CGGGTCATGG TCACCGGCAG 
TCAGCGCCTG TTCCATCGGG GTCATCGAAG GAATGGGAAC AGGATGAGAC GGAGTGCAAG 
ATTGGCAGGC GCAAGTCCTT TATCGAGAAG GATGTTTGGG CCAACCGTCC CCCGACCGAC 
GCCCTTATCA ACAACCTTGA GGACTTCTTC CCCAACTTGG ATGTCGACCA GCCGGTGCTT 
GAGGAAGGCG GTGACACGGA GGTTGAGGTC GATGCCCCGT CACCGATTGC CGAGGTGGAC 
GAGAGCCAAT TCCAGACACG GCCGCAGCCG ATGGCGGAAA CCATGGCTCA GAGCAACACG 
GCGGTTGCCA GCAGTAGCAA CAACAACGCT CCTATGCTCC CGCCGCTAAA CAGGGTTTCT 
TCAGCTTTCA ACGAGAGCGA TACCCTGGGG TCTGATGAGT CTACGCTCAA GGCGCTGGAG 
TCACGTCCAC CGTCGATGGT CTCTGGCAGT GTGCGCCGCT CTAGAGGCCT TGGCCGCATG 
AAGTCTATTC GTGAGGTGGC CCGTGGTGCG CACGAAGCCC ACAAGCGCTA TACGCAAACG 
TCGATGCAGA CGAACGTGGC CACACCGGCA GTTCCCAACG CGACGACCAA CCTCATGCGG 
CGAAAGAGCA CAAAGATGTT CAACGCCAAC ATTGTGCAGA TCGAACCGAG GCGGGGGTCC 
ATTCTGTCTA CCATTCCCCA GGAGACCCTT CCCACGCAAG ACAACATCAA CACGCTACCG 
AAGAGACAAA CGACGTTCCG CTGGTTCAAG GGTCAGCTGA TTGGCAAGGG CACCTTTGGC 
CGCGTCTACC TCGGTATGAA CGCGACAACG GGTGAATTCT TGGCCGTCAA GGAAGTCGAA 
GTGAACCCCA AAGCCGCGCA AGGCGATAAG AAGAAGATGC AAGAGCTGGT GGCCGCGCTC 
GACCAGGAGA TTGACACGAT GCAGCATTTG GATCATGTCA ACATTGTGCA GTACCTCGGC 
TGCGAGCGCA AGGAGACCTC CATCTCCATC TTCCTCGAGT ATATTTCGGG TGGCTCCATC 
GGCTCGTGCC TGCGCAAGCA CGGCAAGTTT GAGGAGCCCG TGGTTGCGTC TTTAACGCGC 
CAGACGCTTT CCGGTCTGGC GTATCTGCAC CGCGAAGGGA TTCTGCATCG TGACCTCAAG 
GCGGACAATA TCTTGCTGGA TCTGGACGGC ACGTGCAAGA TATCCGATTT CGGCATCTCC 
AAGAAGACGG ACAACATCTA TGGTAATGAC AAGACCAACT CGATGCAGGG GTCCGTGTTC 
TGGATGGCGC CCGAGGTGAT CCGGTCGCAG GGCGAAGGAT ACTCGGCCAA GGTGGATATT 
TGGTCGTTGG GCTGTGTGGT GCTGGAGATG TTTGCGGGCA GAAGGCCGTG GAGCAAGGAT 
GAGGCGGTTG GGGCGATTTA CAAGATTGCG AATGGCGAGG CGCCGCCGAT TCCGGATGAT 
ATCCGGGAGG AGATCACGCC GATTGCGATT GCTTTTATGT TGGATTGCTT TACTGTCGAC 
CCAACTGATC GCCCTACCGC CGACGTCCTT CTCTCCCAAC ACCCCTTTTG CGAGCTGGAT 
CCTAACTACA GCTTTATGGA TACGGAGCTG TATGCCAAGA TCAGGGGTAC TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011395111.1
CDS	1..5337
Translation	MYQNGQRDPPRPFAVPPPPPPMSPPQVGSGVAGINSVMNIPPPPPRYPGAPGTVGGSLLPPPPGPPPGNPGFPPSAIAPPSALGAAPWHGAWGRPYNGQTAFNLPPPPPGGNGGLQAYNPLLHAQAAAAAAAAAASASNTPGPPATSITIPPPPPPSEQMSATYIPSAGDTYGEGVGIPGLGLPEELGGPGNWTGAEQQRLGGTSMEDAHRLHAAPTADRGLSTTSNTPNSSSAIPPELAAQWPLDRVLMWLQANSFSQDWVNTFKVLNLHGAQFLELGSGHGGRGNFGMMHQQVYPQLAHECQMSKTGWDQTKEREEGKRMRRMIRGIVTGRSVDPSKVAASGGAQGGAGGAGGGGHARRESVSASVPPSAGPDSADSPNVEFSKSHLFVQEEQVQSGPKAPGPGFAGRRFSQSRATTMPLLTSTMSSGEPNHRSMMRNLDIDSARRHSPSTSNVSESGDLNAGTFRTVGRERQDSPIGGSPNPSSALFPSNAGNTILSSSPHRSGSRFGHRSRNSSDSVSSNAAQYGSGIPADAAAMFKNGSLADMIKNSFNSNNPDRRNGQDGGRPLGVVETGDRSAGTDPPNSAKGPKSFLSFLSRNKRKEDGTSPDELDSPTSPATGFKAHSLGSRAGHVSETSLDQRPGSSISTHEHSVGFQSGHSRKKSVVSTRVYLLATLDHWNYRMLDVTDIDSAHDLRQLVCINLGLPDSEGAAIYVTELGKFDHEEPLDDMKLLTNKRLRADAIGTLKLFVKPGGLTSSPYPGPGTQSGQPSSYVPRDAPPMDEETFNRLNGQRQRSSSSPPTSRQNTISSKDRDEKMPVPEPVEHKAEPPRKHAIKETSPIGETTPFGIVGRKVDFDQPRLSPFEDKRPDHLFPVRKAPAPPGDPSATLKKVNSLSKKTGQGFRPSTAHATQHHHHNAIDHGYHQRRVSTDIRENMTSERQTRRPAAIRGTAEVPSPSGGIGSLLINIGGHLGGIGHPVAGGARALSPNRVASAPVGHGGEMSEQQRARAPSPSLSPNARRRPSESHISQQQYQQNHRPPSSQQHHPHLQHQAQPQPQPQPQQQQQQQQQQQQQQQYQPRQQQTQQPQQQQQAQQPQQQQQAPAQHEESKRKSHGPDVDFSDNDVRFTPPPESESLNASSNHQDYADDQDDSDDSDDGLFAVPISKNKTAETSKTKDNSGGSDEGLGKRPSLKVATDRSKKNLSVAFTTPQASPRMASFTDETEHASVPASSGHGHRQSAPVPSGSSKEWEQDETECKIGRRKSFIEKDVWANRPPTDALINNLEDFFPNLDVDQPVLEEGGDTEVEVDAPSPIAEVDESQFQTRPQPMAETMAQSNTAVASSSNNNAPMLPPLNRVSSAFNESDTLGSDESTLKALESRPPSMVSGSVRRSRGLGRMKSIREVARGAHEAHKRYTQTSMQTNVATPAVPNATTNLMRRKSTKMFNANIVQIEPRRGSILSTIPQETLPTQDNINTLPKRQTTFRWFKGQLIGKGTFGRVYLGMNATTGEFLAVKEVEVNPKAAQGDKKKMQELVAALDQEIDTMQHLDHVNIVQYLGCERKETSISIFLEYISGGSIGSCLRKHGKFEEPVVASLTRQTLSGLAYLHREGILHRDLKADNILLDLDGTCKISDFGISKKTDNIYGNDKTNSMQGSVFWMAPEVIRSQGEGYSAKVDIWSLGCVVLEMFAGRRPWSKDEAVGAIYKIANGEAPPIPDDIREEITPIAIAFMLDCFTVDPTDRPTADVLLSQHPFCELDPNYSFMDTELYAKIRGTY

Target ORF information:

RefSeq Version	XM_011396809.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A MAP kinase kinase kinase (mik-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396809.1

ORF Insert Sequence:

ATGTACCAAA ATGGCCAGAG GGACCCTCCG CGGCCCTTTG CGGTCCCGCC GCCGCCGCCG 
CCCATGTCAC CCCCGCAAGT CGGTTCTGGA GTTGCAGGCA TCAACAGCGT CATGAACATT 
CCTCCTCCAC CCCCTCGCTA CCCGGGCGCA CCAGGAACTG TGGGCGGATC CTTATTGCCG 
CCTCCTCCCG GCCCGCCTCC TGGGAACCCG GGTTTCCCTC CGAGCGCAAT AGCCCCGCCG 
AGCGCCTTGG GCGCTGCACC CTGGCATGGC GCCTGGGGAC GCCCATACAA CGGACAAACC 
GCCTTCAATC TTCCTCCTCC TCCGCCTGGT GGAAATGGCG GGCTACAGGC CTACAATCCC 
TTGTTACATG CGCAGGCGGC TGCAGCGGCG GCAGCGGCGG CTGCGTCTGC TTCCAATACC 
CCAGGTCCCC CCGCTACCTC GATCACGATA CCTCCCCCGC CACCGCCAAG CGAGCAAATG 
AGCGCGACCT ATATTCCATC GGCCGGCGAC ACGTATGGTG AAGGTGTGGG CATTCCTGGC 
TTGGGACTAC CTGAGGAGTT GGGTGGCCCA GGGAACTGGA CCGGGGCCGA ACAGCAGCGC 
TTGGGAGGAA CATCTATGGA GGATGCGCAT AGGTTGCACG CTGCTCCCAC GGCGGACCGG 
GGCTTATCGA CCACCAGCAA CACCCCCAAC AGCTCGTCGG CTATTCCGCC CGAGTTGGCA 
GCGCAGTGGC CGCTTGATAG GGTCCTGATG TGGCTTCAGG CAAACAGCTT TTCCCAGGAC 
TGGGTGAATA CGTTCAAGGT CTTGAACCTT CATGGTGCGC AATTTCTGGA GCTCGGCAGT 
GGTCATGGTG GTCGCGGAAA CTTTGGTATG ATGCATCAGC AAGTATATCC GCAGCTGGCG 
CATGAGTGCC AGATGAGCAA GACGGGCTGG GACCAGACGA AAGAGCGGGA GGAGGGCAAG 
CGGATGCGAC GAATGATCAG GGGGATCGTG ACGGGTCGAT CTGTAGATCC ATCCAAAGTT 
GCCGCAAGTG GAGGCGCGCA AGGAGGAGCA GGAGGAGCAG GAGGAGGAGG CCATGCCCGC 
AGAGAGTCCG TCAGTGCAAG CGTCCCGCCT AGTGCAGGTC CCGATTCTGC AGATTCTCCA 
AACGTGGAGT TCAGCAAATC TCATCTCTTT GTGCAGGAAG AGCAAGTCCA GTCCGGACCC 
AAGGCCCCGG GCCCGGGCTT CGCAGGCAGG AGATTCTCCC AATCGCGCGC AACGACGATG 
CCGCTGCTGA CAAGTACCAT GTCATCAGGT GAGCCAAATC ACCGTAGCAT GATGAGGAAT 
CTGGATATAG ACAGCGCCCG TCGTCACAGC CCGAGCACCA GTAACGTGAG CGAATCGGGC 
GACCTAAACG CAGGTACCTT CCGTACGGTC GGGAGAGAAA GGCAGGATAG TCCGATCGGT 
GGCAGTCCTA ACCCTTCTTC TGCCCTCTTC CCTTCTAACG CTGGCAACAC GATCTTGTCA 
TCGTCACCAC ATCGCAGCGG CAGCAGGTTT GGTCATCGTT CGCGCAACAG CAGTGATTCA 
GTATCCTCAA ACGCAGCACA GTACGGGTCT GGTATTCCTG CCGATGCCGC AGCCATGTTC 
AAGAACGGAA GCCTCGCCGA TATGATTAAG AATAGCTTTA ACTCAAACAA CCCTGATCGT 
CGAAACGGTC AAGATGGTGG TCGGCCCTTG GGTGTCGTTG AAACAGGAGA CAGGAGTGCC 
GGGACTGACC CTCCGAACAG CGCCAAGGGC CCAAAATCGT TCTTGTCCTT CTTGTCGCGA 
AACAAGAGAA AGGAAGATGG AACATCACCC GACGAGCTGG ATTCCCCCAC CAGTCCAGCT 
ACCGGCTTCA AGGCTCACTC GCTTGGCAGT CGTGCCGGTC ATGTCAGCGA AACCTCGCTC 
GATCAGCGCC CCGGTTCAAG TATCTCGACT CACGAGCACA GTGTCGGGTT CCAGAGCGGC 
CATAGTAGGA AGAAGAGCGT GGTATCTACT CGGGTTTATC TCCTCGCCAC TCTGGATCAC 
TGGAATTATC GCATGTTGGA TGTTACTGAT ATCGACTCCG CTCATGACCT GAGACAGCTA 
GTGTGCATCA ACCTGGGCCT ACCTGACAGT GAAGGCGCGG CAATCTACGT GACTGAGCTG 
GGCAAGTTCG ACCATGAGGA GCCCTTGGAT GATATGAAAC TACTCACGAA CAAAAGGCTA 
CGGGCGGACG CAATCGGTAC ACTAAAACTT TTTGTAAAAC CGGGCGGTTT GACGAGTTCG 
CCATACCCAG GTCCCGGAAC CCAAAGCGGT CAGCCATCAT CGTATGTTCC TCGCGACGCC 
CCTCCCATGG ACGAGGAAAC TTTCAACAGG CTAAACGGAC AAAGGCAGCG TTCTAGTTCA 
TCGCCGCCGA CGTCAAGGCA GAATACGATA TCCAGCAAGG ACCGGGACGA GAAGATGCCA 
GTGCCGGAGC CAGTCGAGCA CAAGGCAGAG CCCCCGAGAA AGCACGCAAT CAAGGAGACA 
AGCCCGATCG GAGAGACGAC ACCGTTCGGT ATTGTCGGCA GGAAAGTCGA CTTTGACCAG 
CCCCGACTCT CACCCTTCGA GGACAAGAGG CCCGATCACC TGTTTCCGGT ACGAAAGGCA 
CCTGCACCAC CAGGGGACCC ATCGGCTACT TTGAAAAAGG TCAATTCACT GAGTAAGAAA 
ACTGGACAGG GATTCCGACC TAGCACGGCG CATGCCACCC AGCACCATCA TCATAACGCC 
ATAGATCATG GATACCACCA GAGACGAGTA TCGACGGATA TACGCGAGAA CATGACGTCC 
GAAAGGCAGA CGAGGAGGCC AGCCGCCATA AGAGGAACCG CCGAAGTGCC ATCACCATCA 
GGAGGAATCG GCAGTTTGCT CATCAATATA GGGGGCCACC TGGGTGGCAT CGGTCATCCT 
GTCGCTGGTG GTGCTCGCGC ATTATCACCA AACCGTGTTG CCTCAGCGCC CGTCGGGCAT 
GGTGGTGAGA TGAGCGAACA GCAAAGAGCA CGCGCGCCGT CACCATCACT TAGTCCCAAT 
GCTCGTCGGA GACCAAGCGA GTCTCATATC AGCCAGCAAC AATATCAGCA GAATCACCGA 
CCACCATCAT CGCAGCAGCA TCATCCACAT CTCCAGCACC AGGCACAGCC ACAGCCACAG 
CCACAGCCAC AGCAGCAGCA ACAACAGCAA CAGCAACAAC AACAACAGCA ACAATACCAG 
CCGAGACAAC AGCAGACACA GCAACCACAG CAGCAACAAC AAGCACAGCA ACCACAGCAG 
CAACAACAAG CTCCGGCTCA ACACGAGGAG TCCAAGCGCA AGTCCCATGG TCCAGATGTG 
GATTTCAGCG ATAACGATGT GCGCTTCACC CCACCTCCTG AGAGTGAATC CCTCAACGCC 
AGTTCAAACC ATCAAGACTA CGCAGACGAT CAAGATGATT CGGACGACTC TGATGATGGC 
CTCTTCGCGG TTCCGATCTC GAAAAACAAG ACAGCGGAGA CGTCGAAGAC CAAAGATAAT 
TCGGGCGGGA GCGATGAAGG ACTCGGCAAG CGCCCAAGCT TGAAGGTGGC GACCGATCGC 
TCCAAGAAGA ACTTGTCCGT GGCATTCACT ACTCCTCAGG CCAGCCCCAG AATGGCCTCG 
TTCACGGACG AAACTGAGCA TGCTAGTGTG CCAGCTTCCT CGGGTCATGG TCACCGGCAG 
TCAGCGCCTG TTCCATCGGG GTCATCGAAG GAATGGGAAC AGGATGAGAC GGAGTGCAAG 
ATTGGCAGGC GCAAGTCCTT TATCGAGAAG GATGTTTGGG CCAACCGTCC CCCGACCGAC 
GCCCTTATCA ACAACCTTGA GGACTTCTTC CCCAACTTGG ATGTCGACCA GCCGGTGCTT 
GAGGAAGGCG GTGACACGGA GGTTGAGGTC GATGCCCCGT CACCGATTGC CGAGGTGGAC 
GAGAGCCAAT TCCAGACACG GCCGCAGCCG ATGGCGGAAA CCATGGCTCA GAGCAACACG 
GCGGTTGCCA GCAGTAGCAA CAACAACGCT CCTATGCTCC CGCCGCTAAA CAGGGTTTCT 
TCAGCTTTCA ACGAGAGCGA TACCCTGGGG TCTGATGAGT CTACGCTCAA GGCGCTGGAG 
TCACGTCCAC CGTCGATGGT CTCTGGCAGT GTGCGCCGCT CTAGAGGCCT TGGCCGCATG 
AAGTCTATTC GTGAGGTGGC CCGTGGTGCG CACGAAGCCC ACAAGCGCTA TACGCAAACG 
TCGATGCAGA CGAACGTGGC CACACCGGCA GTTCCCAACG CGACGACCAA CCTCATGCGG 
CGAAAGAGCA CAAAGATGTT CAACGCCAAC ATTGTGCAGA TCGAACCGAG GCGGGGGTCC 
ATTCTGTCTA CCATTCCCCA GGAGACCCTT CCCACGCAAG ACAACATCAA CACGCTACCG 
AAGAGACAAA CGACGTTCCG CTGGTTCAAG GGTCAGCTGA TTGGCAAGGG CACCTTTGGC 
CGCGTCTACC TCGGTATGAA CGCGACAACG GGTGAATTCT TGGCCGTCAA GGAAGTCGAA 
GTGAACCCCA AAGCCGCGCA AGGCGATAAG AAGAAGATGC AAGAGCTGGT GGCCGCGCTC 
GACCAGGAGA TTGACACGAT GCAGCATTTG GATCATGTCA ACATTGTGCA GTACCTCGGC 
TGCGAGCGCA AGGAGACCTC CATCTCCATC TTCCTCGAGT ATATTTCGGG TGGCTCCATC 
GGCTCGTGCC TGCGCAAGCA CGGCAAGTTT GAGGAGCCCG TGGTTGCGTC TTTAACGCGC 
CAGACGCTTT CCGGTCTGGC GTATCTGCAC CGCGAAGGGA TTCTGCATCG TGACCTCAAG 
GCGGACAATA TCTTGCTGGA TCTGGACGGC ACGTGCAAGA TATCCGATTT CGGCATCTCC 
AAGAAGACGG ACAACATCTA TGGTAATGAC AAGACCAACT CGATGCAGGG GTCCGTGTTC 
TGGATGGCGC CCGAGGTGAT CCGGTCGCAG GGCGAAGGAT ACTCGGCCAA GGTGGATATT 
TGGTCGTTGG GCTGTGTGGT GCTGGAGATG TTTGCGGGCA GAAGGCCGTG GAGCAAGGAT 
GAGGCGGTTG GGGCGATTTA CAAGATTGCG AATGGCGAGG CGCCGCCGAT TCCGGATGAT 
ATCCGGGAGG AGATCACGCC GATTGCGATT GCTTTTATGT TGGATTGCTT TACTGTCGAC 
CCAACTGATC GCCCTACCGC CGACGTCCTT CTCTCCCAAC ACCCCTTTTG CGAGCTGGAT 
CCTAACTACA GCTTTATGGA TACGGAGCTG TATGCCAAGA TCAGGGGTAC TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mik-1 ( XM_011396810.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011395112.1 Neurospora crassa OR74A MAP kinase kinase kinase, variant (mik-1), mRNA.

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CloneID	ONf11129
Clone ID Related Accession (Same CDS sequence)	XM_011396810.1
Accession Version	XM_011396810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5256bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	MAP kinase kinase kinase, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026507).

ATGTACCAAA ATGGCCAGAG GGACCCTCCG CGGCCCTTTG CGGTCCCGCC GCCGCCGCCG 
CCCATGTCAC CCCCGCAAGT CGGTTCTGGA GTTGCAGGCA TCAACAGCGT CATGAACATT 
CCTCCTCCAC CCCCTCGCTA CCCGGGCGCA CCAGGAACTG TGGGCGGATC CTTATTGCCG 
CCTCCTCCCG GCCCGCCTCC TGGGAACCCG GGTTTCCCTC CGAGCGCAAT AGCCCCGCCG 
AGCGCCTTGG GCGCTGCACC CTGGCATGGC GCCTGGGGAC GCCCATACAA CGGACAAACC 
GCCTTCAATC TTCCTCCTCC TCCGCCTGGT GGAAATGGCG GGCTACAGGC CTACAATCCC 
TTGTTACATG CGCAGGCGGC TGCAGCGGCG GCAGCGGCGG CTGCGTCTGC TTCCAATACC 
CCAGGTCCCC CCGCTACCTC GATCACGATA CCTCCCCCGC CACCGCCAAG CGAGCAAATG 
AGCGCGACCT ATATTCCATC GGCCGGCGAC ACGTATGGTG AAGGTGTGGG CATTCCTGGC 
TTGGGACTAC CTGAGGAGTT GGGTGGCCCA GGGAACTGGA CCGGGGCCGA ACAGCAGCGC 
TTGGGAGGAA CATCTATGGA GGATGCGCAT AGGTTGCACG CTGCTCCCAC GGCGGACCGG 
GGCTTATCGA CCACCAGCAA CACCCCCAAC AGCTCGTCGG CTATTCCGCC CGAGTTGGCA 
GCGCAGTGGC CGCTTGATAG GGTCCTGATG TGGCTTCAGG CAAACAGCTT TTCCCAGGAC 
TGGGTGAATA CGTTCAAGGT CTTGAACCTT CATGGTGCGC AATTTCTGGA GCTCGGCAGT 
GGTCATGGTG GTCGCGGAAA CTTTGGTATG ATGCATCAGC AAGTATATCC GCAGCTGGCG 
CATGAGTGCC AGATGAGCAA GACGGGCTGG GACCAGACGA AAGAGCGGGA GGAGGGCAAG 
CGGATGCGAC GAATGATCAG GGGGATCGTG ACGGGTCGAT CTGTAGATCC ATCCAAAGTT 
GCCGCAAGTG GAGGCGCGCA AGGAGGAGCA GGAGGAGCAG GAGGAGGAGG CCATGCCCGC 
AGAGAGTCCG TCAGTGCAAG CGTCCCGCCT AGTGCAGGTC CCGATTCTGC AGATTCTCCA 
AACGTGGAGT TCAGCAAATC TCATCTCTTT GTGCAGGAAG AGCAAGTCCA GTCCGGACCC 
AAGGCCCCGG GCCCGGGCTT CGCAGGCAGG AGATTCTCCC AATCGCGCGC AACGACGATG 
CCGCTGCTGA CAAGTACCAT GTCATCAGGT GAGCCAAATC ACCGTAGCAT GATGAGGAAT 
CTGGATATAG ACAGCGCCCG TCGTCACAGC CCGAGCACCA GTAACGTGAG CGAATCGGGC 
GACCTAAACG CAGGTACCTT CCGTACGGTC GGGAGAGAAA GGCAGGATAG TCCGATCGGT 
GGCAGTCCTA ACCCTTCTTC TGCCCTCTTC CCTTCTAACG CTGGCAACAC GATCTTGTCA 
TCGTCACCAC ATCGCAGCGG CAGCAGGTTT GGTCATCGTT CGCGCAACAG CAGTGATTCA 
GTATCCTCAA ACGCAGCACA GTACGGGTCT GGTATTCCTG CCGATGCCGC AGCCATGTTC 
AAGAACGGAA GCCTCGCCGA TATGATTAAG AATAGCTTTA ACTCAAACAA CCCTGATCGT 
CGAAACGGTC AAGATGGTGG TCGGCCCTTG GGTGTCGTTG AAACAGGAGA CAGGAGTGCC 
GGGACTGACC CTCCGAACAG CGCCAAGGGC CCAAAATCGT TCTTGTCCTT CTTGTCGCGA 
AACAAGAGAA AGGAAGATGG AACATCACCC GACGAGCTGG ATTCCCCCAC CAGTCCAGCT 
ACCGGCTTCA AGGCTCACTC GCTTGGCAGT CGTGCCGGTC ATGTCAGCGA AACCTCGCTC 
GATCAGCGCC CCGGTTCAAG TATCTCGACT CACGAGCACA GTGTCGGGTT CCAGAGCGGC 
CATAGTAGGA AGAAGAGCGT GGTATCTACT CGGGTTTATC TCCTCGCCAC TCTGGATCAC 
TGGAATTATC GCATGTTGGA TGTTACTGAT ATCGACTCCG CTCATGACCT GAGACAGCTA 
GTGTGCATCA ACCTGGGCCT ACCTGACAGT GAAGGCGCGG CAATCTACGT GACTGAGCTG 
GGCAAGTTCG ACCATGAGGA GCCCTTGGAT GATATGAAAC TACTCACGAA CAAAAGGCTA 
CGGGCGGACG CAATCGGTAC ACTAAAACTT TTTGTAAAAC CGGGCGGTTT GACGAGTTCG 
CCATACCCAG GTCCCGGAAC CCAAAGCGGT CAGCCATCAT CGTATGTTCC TCGCGACGCC 
CCTCCCATGG ACGAGGAAAC TTTCAACAGG CTAAACGGAC AAAGGCAGCG TTCTAGTTCA 
TCGCCGCCGA CGTCAAGGCA GAATACGATA TCCAGCAAGG ACCGGGACGA GAAGATGCCA 
GTGCCGGAGC CAGTCGAGCA CAAGGCAGAG CCCCCGAGAA AGCACGCAAT CAAGGAGACA 
AGCCCGATCG GAGAGACGAC ACCGTTCGGT ATTGTCGGCA GGAAAGTCGA CTTTGACCAG 
CCCCGACTCT CACCCTTCGA GGACAAGAGG CCCGATCACC TGTTTCCGGG ATTCCGACCT 
AGCACGGCGC ATGCCACCCA GCACCATCAT CATAACGCCA TAGATCATGG ATACCACCAG 
AGACGAGTAT CGACGGATAT ACGCGAGAAC ATGACGTCCG AAAGGCAGAC GAGGAGGCCA 
GCCGCCATAA GAGGAACCGC CGAAGTGCCA TCACCATCAG GAGGAATCGG CAGTTTGCTC 
ATCAATATAG GGGGCCACCT GGGTGGCATC GGTCATCCTG TCGCTGGTGG TGCTCGCGCA 
TTATCACCAA ACCGTGTTGC CTCAGCGCCC GTCGGGCATG GTGGTGAGAT GAGCGAACAG 
CAAAGAGCAC GCGCGCCGTC ACCATCACTT AGTCCCAATG CTCGTCGGAG ACCAAGCGAG 
TCTCATATCA GCCAGCAACA ATATCAGCAG AATCACCGAC CACCATCATC GCAGCAGCAT 
CATCCACATC TCCAGCACCA GGCACAGCCA CAGCCACAGC CACAGCCACA GCAGCAGCAA 
CAACAGCAAC AGCAACAACA ACAACAGCAA CAATACCAGC CGAGACAACA GCAGACACAG 
CAACCACAGC AGCAACAACA AGCACAGCAA CCACAGCAGC AACAACAAGC TCCGGCTCAA 
CACGAGGAGT CCAAGCGCAA GTCCCATGGT CCAGATGTGG ATTTCAGCGA TAACGATGTG 
CGCTTCACCC CACCTCCTGA GAGTGAATCC CTCAACGCCA GTTCAAACCA TCAAGACTAC 
GCAGACGATC AAGATGATTC GGACGACTCT GATGATGGCC TCTTCGCGGT TCCGATCTCG 
AAAAACAAGA CAGCGGAGAC GTCGAAGACC AAAGATAATT CGGGCGGGAG CGATGAAGGA 
CTCGGCAAGC GCCCAAGCTT GAAGGTGGCG ACCGATCGCT CCAAGAAGAA CTTGTCCGTG 
GCATTCACTA CTCCTCAGGC CAGCCCCAGA ATGGCCTCGT TCACGGACGA AACTGAGCAT 
GCTAGTGTGC CAGCTTCCTC GGGTCATGGT CACCGGCAGT CAGCGCCTGT TCCATCGGGG 
TCATCGAAGG AATGGGAACA GGATGAGACG GAGTGCAAGA TTGGCAGGCG CAAGTCCTTT 
ATCGAGAAGG ATGTTTGGGC CAACCGTCCC CCGACCGACG CCCTTATCAA CAACCTTGAG 
GACTTCTTCC CCAACTTGGA TGTCGACCAG CCGGTGCTTG AGGAAGGCGG TGACACGGAG 
GTTGAGGTCG ATGCCCCGTC ACCGATTGCC GAGGTGGACG AGAGCCAATT CCAGACACGG 
CCGCAGCCGA TGGCGGAAAC CATGGCTCAG AGCAACACGG CGGTTGCCAG CAGTAGCAAC 
AACAACGCTC CTATGCTCCC GCCGCTAAAC AGGGTTTCTT CAGCTTTCAA CGAGAGCGAT 
ACCCTGGGGT CTGATGAGTC TACGCTCAAG GCGCTGGAGT CACGTCCACC GTCGATGGTC 
TCTGGCAGTG TGCGCCGCTC TAGAGGCCTT GGCCGCATGA AGTCTATTCG TGAGGTGGCC 
CGTGGTGCGC ACGAAGCCCA CAAGCGCTAT ACGCAAACGT CGATGCAGAC GAACGTGGCC 
ACACCGGCAG TTCCCAACGC GACGACCAAC CTCATGCGGC GAAAGAGCAC AAAGATGTTC 
AACGCCAACA TTGTGCAGAT CGAACCGAGG CGGGGGTCCA TTCTGTCTAC CATTCCCCAG 
GAGACCCTTC CCACGCAAGA CAACATCAAC ACGCTACCGA AGAGACAAAC GACGTTCCGC 
TGGTTCAAGG GTCAGCTGAT TGGCAAGGGC ACCTTTGGCC GCGTCTACCT CGGTATGAAC 
GCGACAACGG GTGAATTCTT GGCCGTCAAG GAAGTCGAAG TGAACCCCAA AGCCGCGCAA 
GGCGATAAGA AGAAGATGCA AGAGCTGGTG GCCGCGCTCG ACCAGGAGAT TGACACGATG 
CAGCATTTGG ATCATGTCAA CATTGTGCAG TACCTCGGCT GCGAGCGCAA GGAGACCTCC 
ATCTCCATCT TCCTCGAGTA TATTTCGGGT GGCTCCATCG GCTCGTGCCT GCGCAAGCAC 
GGCAAGTTTG AGGAGCCCGT GGTTGCGTCT TTAACGCGCC AGACGCTTTC CGGTCTGGCG 
TATCTGCACC GCGAAGGGAT TCTGCATCGT GACCTCAAGG CGGACAATAT CTTGCTGGAT 
CTGGACGGCA CGTGCAAGAT ATCCGATTTC GGCATCTCCA AGAAGACGGA CAACATCTAT 
GGTAATGACA AGACCAACTC GATGCAGGGG TCCGTGTTCT GGATGGCGCC CGAGGTGATC 
CGGTCGCAGG GCGAAGGATA CTCGGCCAAG GTGGATATTT GGTCGTTGGG CTGTGTGGTG 
CTGGAGATGT TTGCGGGCAG AAGGCCGTGG AGCAAGGATG AGGCGGTTGG GGCGATTTAC 
AAGATTGCGA ATGGCGAGGC GCCGCCGATT CCGGATGATA TCCGGGAGGA GATCACGCCG 
ATTGCGATTG CTTTTATGTT GGATTGCTTT ACTGTCGACC CAACTGATCG CCCTACCGCC 
GACGTCCTTC TCTCCCAACA CCCCTTTTGC GAGCTGGATC CTAACTACAG CTTTATGGAT 
ACGGAGCTGT ATGCCAAGAT CAGGGGTACT TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011395112.1
CDS	1186..6441
Translation	MYQNGQRDPPRPFAVPPPPPPMSPPQVGSGVAGINSVMNIPPPPPRYPGAPGTVGGSLLPPPPGPPPGNPGFPPSAIAPPSALGAAPWHGAWGRPYNGQTAFNLPPPPPGGNGGLQAYNPLLHAQAAAAAAAAAASASNTPGPPATSITIPPPPPPSEQMSATYIPSAGDTYGEGVGIPGLGLPEELGGPGNWTGAEQQRLGGTSMEDAHRLHAAPTADRGLSTTSNTPNSSSAIPPELAAQWPLDRVLMWLQANSFSQDWVNTFKVLNLHGAQFLELGSGHGGRGNFGMMHQQVYPQLAHECQMSKTGWDQTKEREEGKRMRRMIRGIVTGRSVDPSKVAASGGAQGGAGGAGGGGHARRESVSASVPPSAGPDSADSPNVEFSKSHLFVQEEQVQSGPKAPGPGFAGRRFSQSRATTMPLLTSTMSSGEPNHRSMMRNLDIDSARRHSPSTSNVSESGDLNAGTFRTVGRERQDSPIGGSPNPSSALFPSNAGNTILSSSPHRSGSRFGHRSRNSSDSVSSNAAQYGSGIPADAAAMFKNGSLADMIKNSFNSNNPDRRNGQDGGRPLGVVETGDRSAGTDPPNSAKGPKSFLSFLSRNKRKEDGTSPDELDSPTSPATGFKAHSLGSRAGHVSETSLDQRPGSSISTHEHSVGFQSGHSRKKSVVSTRVYLLATLDHWNYRMLDVTDIDSAHDLRQLVCINLGLPDSEGAAIYVTELGKFDHEEPLDDMKLLTNKRLRADAIGTLKLFVKPGGLTSSPYPGPGTQSGQPSSYVPRDAPPMDEETFNRLNGQRQRSSSSPPTSRQNTISSKDRDEKMPVPEPVEHKAEPPRKHAIKETSPIGETTPFGIVGRKVDFDQPRLSPFEDKRPDHLFPGFRPSTAHATQHHHHNAIDHGYHQRRVSTDIRENMTSERQTRRPAAIRGTAEVPSPSGGIGSLLINIGGHLGGIGHPVAGGARALSPNRVASAPVGHGGEMSEQQRARAPSPSLSPNARRRPSESHISQQQYQQNHRPPSSQQHHPHLQHQAQPQPQPQPQQQQQQQQQQQQQQQYQPRQQQTQQPQQQQQAQQPQQQQQAPAQHEESKRKSHGPDVDFSDNDVRFTPPPESESLNASSNHQDYADDQDDSDDSDDGLFAVPISKNKTAETSKTKDNSGGSDEGLGKRPSLKVATDRSKKNLSVAFTTPQASPRMASFTDETEHASVPASSGHGHRQSAPVPSGSSKEWEQDETECKIGRRKSFIEKDVWANRPPTDALINNLEDFFPNLDVDQPVLEEGGDTEVEVDAPSPIAEVDESQFQTRPQPMAETMAQSNTAVASSSNNNAPMLPPLNRVSSAFNESDTLGSDESTLKALESRPPSMVSGSVRRSRGLGRMKSIREVARGAHEAHKRYTQTSMQTNVATPAVPNATTNLMRRKSTKMFNANIVQIEPRRGSILSTIPQETLPTQDNINTLPKRQTTFRWFKGQLIGKGTFGRVYLGMNATTGEFLAVKEVEVNPKAAQGDKKKMQELVAALDQEIDTMQHLDHVNIVQYLGCERKETSISIFLEYISGGSIGSCLRKHGKFEEPVVASLTRQTLSGLAYLHREGILHRDLKADNILLDLDGTCKISDFGISKKTDNIYGNDKTNSMQGSVFWMAPEVIRSQGEGYSAKVDIWSLGCVVLEMFAGRRPWSKDEAVGAIYKIANGEAPPIPDDIREEITPIAIAFMLDCFTVDPTDRPTADVLLSQHPFCELDPNYSFMDTELYAKIRGTY

Target ORF information:

RefSeq Version	XM_011396810.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A MAP kinase kinase kinase, variant (mik-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396810.1

ORF Insert Sequence:

ATGTACCAAA ATGGCCAGAG GGACCCTCCG CGGCCCTTTG CGGTCCCGCC GCCGCCGCCG 
CCCATGTCAC CCCCGCAAGT CGGTTCTGGA GTTGCAGGCA TCAACAGCGT CATGAACATT 
CCTCCTCCAC CCCCTCGCTA CCCGGGCGCA CCAGGAACTG TGGGCGGATC CTTATTGCCG 
CCTCCTCCCG GCCCGCCTCC TGGGAACCCG GGTTTCCCTC CGAGCGCAAT AGCCCCGCCG 
AGCGCCTTGG GCGCTGCACC CTGGCATGGC GCCTGGGGAC GCCCATACAA CGGACAAACC 
GCCTTCAATC TTCCTCCTCC TCCGCCTGGT GGAAATGGCG GGCTACAGGC CTACAATCCC 
TTGTTACATG CGCAGGCGGC TGCAGCGGCG GCAGCGGCGG CTGCGTCTGC TTCCAATACC 
CCAGGTCCCC CCGCTACCTC GATCACGATA CCTCCCCCGC CACCGCCAAG CGAGCAAATG 
AGCGCGACCT ATATTCCATC GGCCGGCGAC ACGTATGGTG AAGGTGTGGG CATTCCTGGC 
TTGGGACTAC CTGAGGAGTT GGGTGGCCCA GGGAACTGGA CCGGGGCCGA ACAGCAGCGC 
TTGGGAGGAA CATCTATGGA GGATGCGCAT AGGTTGCACG CTGCTCCCAC GGCGGACCGG 
GGCTTATCGA CCACCAGCAA CACCCCCAAC AGCTCGTCGG CTATTCCGCC CGAGTTGGCA 
GCGCAGTGGC CGCTTGATAG GGTCCTGATG TGGCTTCAGG CAAACAGCTT TTCCCAGGAC 
TGGGTGAATA CGTTCAAGGT CTTGAACCTT CATGGTGCGC AATTTCTGGA GCTCGGCAGT 
GGTCATGGTG GTCGCGGAAA CTTTGGTATG ATGCATCAGC AAGTATATCC GCAGCTGGCG 
CATGAGTGCC AGATGAGCAA GACGGGCTGG GACCAGACGA AAGAGCGGGA GGAGGGCAAG 
CGGATGCGAC GAATGATCAG GGGGATCGTG ACGGGTCGAT CTGTAGATCC ATCCAAAGTT 
GCCGCAAGTG GAGGCGCGCA AGGAGGAGCA GGAGGAGCAG GAGGAGGAGG CCATGCCCGC 
AGAGAGTCCG TCAGTGCAAG CGTCCCGCCT AGTGCAGGTC CCGATTCTGC AGATTCTCCA 
AACGTGGAGT TCAGCAAATC TCATCTCTTT GTGCAGGAAG AGCAAGTCCA GTCCGGACCC 
AAGGCCCCGG GCCCGGGCTT CGCAGGCAGG AGATTCTCCC AATCGCGCGC AACGACGATG 
CCGCTGCTGA CAAGTACCAT GTCATCAGGT GAGCCAAATC ACCGTAGCAT GATGAGGAAT 
CTGGATATAG ACAGCGCCCG TCGTCACAGC CCGAGCACCA GTAACGTGAG CGAATCGGGC 
GACCTAAACG CAGGTACCTT CCGTACGGTC GGGAGAGAAA GGCAGGATAG TCCGATCGGT 
GGCAGTCCTA ACCCTTCTTC TGCCCTCTTC CCTTCTAACG CTGGCAACAC GATCTTGTCA 
TCGTCACCAC ATCGCAGCGG CAGCAGGTTT GGTCATCGTT CGCGCAACAG CAGTGATTCA 
GTATCCTCAA ACGCAGCACA GTACGGGTCT GGTATTCCTG CCGATGCCGC AGCCATGTTC 
AAGAACGGAA GCCTCGCCGA TATGATTAAG AATAGCTTTA ACTCAAACAA CCCTGATCGT 
CGAAACGGTC AAGATGGTGG TCGGCCCTTG GGTGTCGTTG AAACAGGAGA CAGGAGTGCC 
GGGACTGACC CTCCGAACAG CGCCAAGGGC CCAAAATCGT TCTTGTCCTT CTTGTCGCGA 
AACAAGAGAA AGGAAGATGG AACATCACCC GACGAGCTGG ATTCCCCCAC CAGTCCAGCT 
ACCGGCTTCA AGGCTCACTC GCTTGGCAGT CGTGCCGGTC ATGTCAGCGA AACCTCGCTC 
GATCAGCGCC CCGGTTCAAG TATCTCGACT CACGAGCACA GTGTCGGGTT CCAGAGCGGC 
CATAGTAGGA AGAAGAGCGT GGTATCTACT CGGGTTTATC TCCTCGCCAC TCTGGATCAC 
TGGAATTATC GCATGTTGGA TGTTACTGAT ATCGACTCCG CTCATGACCT GAGACAGCTA 
GTGTGCATCA ACCTGGGCCT ACCTGACAGT GAAGGCGCGG CAATCTACGT GACTGAGCTG 
GGCAAGTTCG ACCATGAGGA GCCCTTGGAT GATATGAAAC TACTCACGAA CAAAAGGCTA 
CGGGCGGACG CAATCGGTAC ACTAAAACTT TTTGTAAAAC CGGGCGGTTT GACGAGTTCG 
CCATACCCAG GTCCCGGAAC CCAAAGCGGT CAGCCATCAT CGTATGTTCC TCGCGACGCC 
CCTCCCATGG ACGAGGAAAC TTTCAACAGG CTAAACGGAC AAAGGCAGCG TTCTAGTTCA 
TCGCCGCCGA CGTCAAGGCA GAATACGATA TCCAGCAAGG ACCGGGACGA GAAGATGCCA 
GTGCCGGAGC CAGTCGAGCA CAAGGCAGAG CCCCCGAGAA AGCACGCAAT CAAGGAGACA 
AGCCCGATCG GAGAGACGAC ACCGTTCGGT ATTGTCGGCA GGAAAGTCGA CTTTGACCAG 
CCCCGACTCT CACCCTTCGA GGACAAGAGG CCCGATCACC TGTTTCCGGG ATTCCGACCT 
AGCACGGCGC ATGCCACCCA GCACCATCAT CATAACGCCA TAGATCATGG ATACCACCAG 
AGACGAGTAT CGACGGATAT ACGCGAGAAC ATGACGTCCG AAAGGCAGAC GAGGAGGCCA 
GCCGCCATAA GAGGAACCGC CGAAGTGCCA TCACCATCAG GAGGAATCGG CAGTTTGCTC 
ATCAATATAG GGGGCCACCT GGGTGGCATC GGTCATCCTG TCGCTGGTGG TGCTCGCGCA 
TTATCACCAA ACCGTGTTGC CTCAGCGCCC GTCGGGCATG GTGGTGAGAT GAGCGAACAG 
CAAAGAGCAC GCGCGCCGTC ACCATCACTT AGTCCCAATG CTCGTCGGAG ACCAAGCGAG 
TCTCATATCA GCCAGCAACA ATATCAGCAG AATCACCGAC CACCATCATC GCAGCAGCAT 
CATCCACATC TCCAGCACCA GGCACAGCCA CAGCCACAGC CACAGCCACA GCAGCAGCAA 
CAACAGCAAC AGCAACAACA ACAACAGCAA CAATACCAGC CGAGACAACA GCAGACACAG 
CAACCACAGC AGCAACAACA AGCACAGCAA CCACAGCAGC AACAACAAGC TCCGGCTCAA 
CACGAGGAGT CCAAGCGCAA GTCCCATGGT CCAGATGTGG ATTTCAGCGA TAACGATGTG 
CGCTTCACCC CACCTCCTGA GAGTGAATCC CTCAACGCCA GTTCAAACCA TCAAGACTAC 
GCAGACGATC AAGATGATTC GGACGACTCT GATGATGGCC TCTTCGCGGT TCCGATCTCG 
AAAAACAAGA CAGCGGAGAC GTCGAAGACC AAAGATAATT CGGGCGGGAG CGATGAAGGA 
CTCGGCAAGC GCCCAAGCTT GAAGGTGGCG ACCGATCGCT CCAAGAAGAA CTTGTCCGTG 
GCATTCACTA CTCCTCAGGC CAGCCCCAGA ATGGCCTCGT TCACGGACGA AACTGAGCAT 
GCTAGTGTGC CAGCTTCCTC GGGTCATGGT CACCGGCAGT CAGCGCCTGT TCCATCGGGG 
TCATCGAAGG AATGGGAACA GGATGAGACG GAGTGCAAGA TTGGCAGGCG CAAGTCCTTT 
ATCGAGAAGG ATGTTTGGGC CAACCGTCCC CCGACCGACG CCCTTATCAA CAACCTTGAG 
GACTTCTTCC CCAACTTGGA TGTCGACCAG CCGGTGCTTG AGGAAGGCGG TGACACGGAG 
GTTGAGGTCG ATGCCCCGTC ACCGATTGCC GAGGTGGACG AGAGCCAATT CCAGACACGG 
CCGCAGCCGA TGGCGGAAAC CATGGCTCAG AGCAACACGG CGGTTGCCAG CAGTAGCAAC 
AACAACGCTC CTATGCTCCC GCCGCTAAAC AGGGTTTCTT CAGCTTTCAA CGAGAGCGAT 
ACCCTGGGGT CTGATGAGTC TACGCTCAAG GCGCTGGAGT CACGTCCACC GTCGATGGTC 
TCTGGCAGTG TGCGCCGCTC TAGAGGCCTT GGCCGCATGA AGTCTATTCG TGAGGTGGCC 
CGTGGTGCGC ACGAAGCCCA CAAGCGCTAT ACGCAAACGT CGATGCAGAC GAACGTGGCC 
ACACCGGCAG TTCCCAACGC GACGACCAAC CTCATGCGGC GAAAGAGCAC AAAGATGTTC 
AACGCCAACA TTGTGCAGAT CGAACCGAGG CGGGGGTCCA TTCTGTCTAC CATTCCCCAG 
GAGACCCTTC CCACGCAAGA CAACATCAAC ACGCTACCGA AGAGACAAAC GACGTTCCGC 
TGGTTCAAGG GTCAGCTGAT TGGCAAGGGC ACCTTTGGCC GCGTCTACCT CGGTATGAAC 
GCGACAACGG GTGAATTCTT GGCCGTCAAG GAAGTCGAAG TGAACCCCAA AGCCGCGCAA 
GGCGATAAGA AGAAGATGCA AGAGCTGGTG GCCGCGCTCG ACCAGGAGAT TGACACGATG 
CAGCATTTGG ATCATGTCAA CATTGTGCAG TACCTCGGCT GCGAGCGCAA GGAGACCTCC 
ATCTCCATCT TCCTCGAGTA TATTTCGGGT GGCTCCATCG GCTCGTGCCT GCGCAAGCAC 
GGCAAGTTTG AGGAGCCCGT GGTTGCGTCT TTAACGCGCC AGACGCTTTC CGGTCTGGCG 
TATCTGCACC GCGAAGGGAT TCTGCATCGT GACCTCAAGG CGGACAATAT CTTGCTGGAT 
CTGGACGGCA CGTGCAAGAT ATCCGATTTC GGCATCTCCA AGAAGACGGA CAACATCTAT 
GGTAATGACA AGACCAACTC GATGCAGGGG TCCGTGTTCT GGATGGCGCC CGAGGTGATC 
CGGTCGCAGG GCGAAGGATA CTCGGCCAAG GTGGATATTT GGTCGTTGGG CTGTGTGGTG 
CTGGAGATGT TTGCGGGCAG AAGGCCGTGG AGCAAGGATG AGGCGGTTGG GGCGATTTAC 
AAGATTGCGA ATGGCGAGGC GCCGCCGATT CCGGATGATA TCCGGGAGGA GATCACGCCG 
ATTGCGATTG CTTTTATGTT GGATTGCTTT ACTGTCGACC CAACTGATCG CCCTACCGCC 
GACGTCCTTC TCTCCCAACA CCCCTTTTGC GAGCTGGATC CTAACTACAG CTTTATGGAT 
ACGGAGCTGT ATGCCAAGAT CAGGGGTACT TATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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mik-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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