NCU02083 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU02083
Entrez Gene ID	3880750
Full Name	1-phosphatidylinositol-3-phosphate 5-kinase
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_959517.2	XP_964610.1	1-phosphatidylinositol-3-phosphate 5-kinase

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_14757	XP_003713896.1
Neurospora crassa	NCU02083	XP_964610.1
Schizosaccharomyces pombe (fission yeast)	fab1	NP_596090.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU02083 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU02083 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf07915	XM_959517.2 Latest version!	Neurospora crassa OR74A 1-phosphatidylinositol-3-phosphate 5-kinase (NCU02083), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU02083 ( XM_959517.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_964610.1 Neurospora crassa OR74A 1-phosphatidylinositol-3-phosphate 5-kinase (NCU02083), mRNA.

Price & Availability↑

CloneID	ONf07915
Clone ID Related Accession (Same CDS sequence)	XM_959517.2
Accession Version	XM_959517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	1-phosphatidylinositol-3-phosphate 5-kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026501). On Feb 23, 2015 this sequence version replaced XM_959517.1.

ATGGCTTCCA GCTCACACAG GCCATTCAAG CTCCCCCTTG GTCCCCTTTC GCGCTCCAAC 
AATGGCGACC ATTCCTCCGA GTCCGACTAT CCGGGCGGCC GTCGCGACTC CATGGCCGCC 
CTCTCCATCT CTTCTCATGT CAACCAGGAA CAGCTCGCTC ACACCCTTGA CCGAATTCAC 
AATACAGCAA GCCAATCTGG GGTCCTGACC ACCTTCAATG ATTTCGCCCC GCCACCTCCA 
CCTACTGTGC CTGTTGCTGA GAAAAAGGGA ACTACGGGTG ACTTGGTACA GCAAGGCTTG 
AGTGGTCTCT ACAGCCGTTT AAAGGAGGTT GTTGGGGTGG CAGGGAAACC TTCTGCCCAG 
GAGGCTGAAA GTTCGACTAA CACCCAGGAT GCCACGCCCA AGAATTTCAA GAGCCCGATT 
CCCAATAGCC CCAACAACAG TAGCACCAAG ATCTCGGTTG CCTCCCCTCC AAAGGGAGAC 
GGTTCGTCCA TCAAGTCACC TTTTGATACA AAGTCGCCTT TTGATACAAA GTCGCCCTTC 
GATGCAAAAT CACCCTTTGA AGCAAGGCAT GCCTTTGATG GGCCCGCCTC TCTTGACATC 
CCACCCGCTG TGTCTACCGG CACATCTCCA ATTTCGCCAA CCACCATACC AGCAGACCTC 
CAGTCCCCCC CACAGACAAC CAGGCCACCA TCACTTGGTA CGCTGCCGAG CCTCAAGACC 
GGAAACTCAA GCGCACAGAC AATTCCGAGG ACGGGAAGGA CGTCCCAGTC AACCGCGGCC 
AGCCCTACCT CTGCCCCCTT CATGGGAAAA GCGTCTCTCG AAAGAGACAG CTCTAGGGGT 
CCTGCCAGAC ACCTCGATGA GCCCTCAAGT TGGGGTTCTA GCCGGCGGAG CATCAGCAGA 
CATGGTGACG GATCCACGTC GCCTAGCATT GCCGTGAGAC CAAGTGTAGA TACCACGACG 
GCCGCTTTGG CGCGCAGTAG AATGGAGGAC GCTGTGAGTA TAGATGGAGG CATCGGCAGT 
CCCATGAGCC CTTCTGGGCC TATACCAGAC GTGTGCATCT CCTCTGCCTC CTCGCGCTTG 
TCTGAGGCCA CTCAAGTCAA GCGAAGGCCT GCCATTATTG ACCGCATAAG CCAGTCCAGA 
GTTCATAGCC ATTCCAGGTC CTCGTCCATG GAGCCTGGCA AGGCACAGCC CAGTCCCATC 
AGCACATCGG CTCATAGCAC TATCTATCAT GATTCGTTTA CTCACAATGT CAAACCACAG 
AGGTTGCAAT CTGGAGCAAT GAAAATCCCA GGCACCACGA CAAACGAAGG AGCCCCTGAG 
GTCGTCAATG CCAGACTAGA AATGATGAGG AAGCAGGTCC TTAGCAAAGA GTTCTGGATG 
AAAGACGAAA CCACCAAAGA ATGCTTTGCC TGCGGGAGCC CGTTCACTGC CTTTCGGCGC 
AAGCACCACT GCCGCACGTG TGGCTGCATC TTCTGTTCAA AATGCACCTC TACCATTTCA 
GGACAAAAGT TTGGCGTCCA GGGAACGCTT CGCGTCTGCA AGAATTGCTT GGAGGTCATA 
AACCGCCGCT ATGAAAGCGG CTCAGATGAT TCGGCAGATG AGCCATTCTT GCCCGCCATC 
TTTCGTTCCA ATCAGACCAA GCCGCCTCCT CTTGCTCTTG CTCGACCGCA GGCGGATGAT 
GAGGTTAGTA TCGCGGAGCG AACGGAGCAA GCAAATGACA CAAGGTCCGC CAAGACACCG 
ATGATGGCGA TTCCGGCCAC ACGCCGAGTT GGCGACAGCA ATCGCCACTC GGCCGTTTTG 
GAGATTGATA TGCCACAGCT CAGCCGCCCC GGATCATCTC GGTCGCTGAG ATCACTCGCG 
GCGGGCCGAC CGCAATCATC TAGTCATCGC AGGCTTCATT CAAAACATAC CTTCTTGACT 
AGGTTCAAGT CTTCTGCGAC TGACGATAGG GCACCGTTTC GCAAGCCTGC TAGCGAAGAC 
CTTGGGAGGA AATCCAAGCT CAGCGCCTTC CATGACGACA ACATCATTGA TCCAGAACTT 
GCCCCTTACA TGTCGGACGA GTCAACTGAT GACGAACAAA TGAGTAGCAT ATTTGCAACC 
ATGAACAGCA ATGATGCGCC AGCGGCCAGC CTCGATCCCG ACAAAACAAG CATGGGTGCC 
TTTATGAATG CTGCAAACAG AAAACATCGG TTCCGTCACG GCGAGAAGAG CATCAGTGGA 
CTTAGTTATA CCAGCAGAGG CCACGAAGAC ACTGGCTTAC CGACTCTCTC TCTCCACACC 
CGGCCAACCA GAAAGAGAAA TCTCAGCACT GCAAGTGTCA CGGGCCATCC GCTGCGGTCA 
CCCCGCCCAA GATCTGGCAT CTTTAGCATG ATGCTAAACC CTGGTGCTTC AGCTGACGCC 
TTGTCTCTCT GGGATAATCC TGGAGTTGTC GAAGCATCTG CACTCAGTAG AAGGGATTCT 
TACTTTGAGG GCAGGTCATC AACCGGTGGA TTAAACCCCA TAAGCATGAA CCACGTCAAG 
AAGCTTTTCC GGCAGCTTTT GAACGATGCA GAAATCCCCA ACCCTGCAGC GTGGGAAAAG 
GCCCTCATCC CAATCCTTGA TAGATGTGCT GATGACGTTG ATCCGGATGT CCGTAACGGA 
GATGACATGG ACATTCGCCA CTGGATCAAG TTGAAAAAGA TACCTGGCGG CAAACCTGGC 
GATACTGCTT ACGTCCATGG AGTCGTCTTT TCCAAGAACT TGGCCCTCAA GACCATGCCC 
CGCAAGATCG AGAATCCCAA AATCGTCATC ATCACTTTTC CAATCGAGTA TCAGCGGCAC 
CAGGAGCAGC ATTTTATGAG CTTGCAGCCG GTCATAGAGC AGGAAAAGGA GTATCTGAGG 
ATGGTGGTGA ACCGAATCCT GAACCTCGAG CCTCGGGTGT TGCTCGTCGA AAAGAACGTC 
TCTGGCGTGG CTTTACAATA TCTCTCGGAA GCCAACGTCG CGGTCGTGTA TAACGTCAAA 
CCGTCAGTTA TCGAGGCTGT TTCCAGAATT GCCAATATTC CCATCATCTC ATCCATGGAT 
ATGCTTAGCT TGGGTGCGCG CGTCGGGACC TGCCAAAGTT TTGAAGTCAA GACCTTCGTC 
AACAAGGAGC TCAAAGGCAG GAAGAAGACG TACATTTTTA TCTCTGGCTG CCCAAAAGAG 
CGCGGCTGTA CTATTGCTCT TCGCGGCGGG TCGACAGAGA TTCTGTCCCG CATGAAGAGA 
ATCACCGAGT TCATGGTTTA TGTGTTTTAC AACCTAAAGC TCGAATCCTG TCTTATGCGC 
GATGAATTTG TTTATGTGCC TGAAGAGCTC GAGTCCCCTC CACGATCAAC TACGAAGCTC 
CTGAGTAACG GCAGTCAGGA CCCGTCGTCA ACTACTCCAA GCGCGCAGAC TGGGGAAAAT 
TCCCCTCACA GCGTGATCCA CCACATTTCC GAACATGATC AGTCTTTGGA ACCATCTCAA 
GCGGCTGCTA GACCTGTCGG GAGCACAACT TCGGCCGAGG AATATTCAGC TGCCGCCCTC 
GACACCAGCA CGTCGATCAA GGAAGAGACT GAGGAGGAAT TGCTTCGGGC TGAGTCAGAC 
ACCCATGAAG GTCAAGGTGC GGAAGACTTG CCTATGCCGA CATATTATGG TGACATGGTA 
GCCAAATACG AGACCAGAAT TCTCTCTGCG TCACCCTTCG TCAAGTTCAC TCAACCTTAC 
CTGCTCATGA AAGCCCGAGA GCAGGAGAGG CGCCTGGTGT ATCTCAAACG TCTTCGTGAC 
CAGGACATCG TCGAAGAACT CGGAGAGAGC GAGAAGAGTG AGCCTCAGCC TAGACAATTT 
CAGCTTATCA AGCCTGAAAT GGTGCATGAA CTTGGCCAAA AGGCACCGCG CCACATCATG 
GAGGTGCTCC ATGCTGTCCA CGACGCGGAA TACGACAAGG CCTTGTACAA CTATCAGACT 
CAAACCAGAC AGTGGGAGAA TTATATCCAG GGAAGCCTCG ACCTGTTTGA TCCATACTCC 
CATCAGAGCA TCGTTATTCT GTACTCTGTC ACATGCAACG TGACCCAAGT TCCATGCACG 
GAGCCCAGCG TGGTGGCCTT TGAGTTCTAC AAGGAGAATC CAGACCCACT CAGTGGAATG 
TACCAAGATT GCACCCTGGG GCAGTACATA GAAGACGTCT GCGAAAGCGC CGACTCAATC 
TGTCACTCCA ATGGATGCGA CCGAAAGATG TTTGAGCATT ACCAGACATA TGTTCACGAA 
AACGCGCGAA TTACTGTCAT GGTGGAGGAC AAGCCCAAAT GGCCAGAGAA CTTCCCCGAG 
AAACCGCACG AAAGGGATGG TTGGCAAGAT GGCACCGGCA TCTGCATGTG GAATTACTGC 
AAGTTATGCA ACAAACATTT CGGACTGATG CCCATGTCAG TGAGCACCTG GAAGTATTCT 
TTCGCAAAGT ATCTGGAACT GTCGTTCTGG AGCCGTGGTT TACGCCTTCA CCCAGAGACA 
GGATGTCCTC ACGATCATAA AAAAGACCAT GTTCGCTTCT TTTATTACCT ATACCGCGAC 
ATTGCCGTCA GGATACACTA CGACCCGATC GACTTGTACG AAATCATTGT ACCTAGATCT 
CGCATCACCT GGAAGGTTGA CCATGATCTG GAGTTGAAAA ATCAAGTCTT TCTCAAGGCC 
GAGGAGCGCT GGAACCGCTT CATCAACTCC GTAAAAGCCA GGCTGAAGAG CATTAGAATC 
GATAGCGTCT TGCCGGAGAA AACGGAGCAT TGCAAGGCTG AGGTGGAAAG AATGACAAAG 
AAGGCACAAG AGGACCAGGC CGAGCTGATT CAGGACCTTC AGGATGCCTA CATGGGCTCA 
AAGTACTACG AGGTTCTTCC CATGAACGGA GTCATACGTC AAATGGCTGA GAGAGCTACT 
GAATGGGACG TTGCCTTCAC TAAATTTGAG GCCGACTTTC TTTCGGACAA AGATGTTCGC 
CAGTTGACTA TCATCCAGCT GAAGAAGATG TTTGACCCAA AATCTGGATC CTCAGGTGCG 
GAACTTGGGG AGAAGCCTGA GGTAACTCAC TCCAATGTCA ACGCCGGCGA AATTGAGGTG 
AAAGAAAAGC CATCGCAGCC AACGGAAGAT GAAGGCCCCA GTCCCAAGAT GCATTCTCCA 
GTGTCTTCAG CGACCAAACC TATTCCTGTG GTGAGGGAGG AAGCAGTAGA AGAGGCCGGG 
TCCATCCTGG ATGGAGTTGA GCATCTTGAC CTTGCAACTG CATCCTCGCC CACGCTCCTC 
AGGAAGGGGG ATCCCGTCCT GGAAGCAGCA AAGGTCACTG TCAATGAGCC AACGCCGATT 
GTTGCACCAG CAGCCCCTAC ACAACAACCA ACATCTCTCT CAAAAGTTGA CGAAGCGCCT 
CATGAGTCGT CTCTAGCAGA GATGATCGAA CCCAAACGCC GAGAACAACA TGTAGCCATG 
CCTGAGGCTA CCACCCATGC TGCCGGCGGC GAGGGTTCGA TGGAAGGTTA CAAGCCCGTT 
GTACCGGAGC GCACGTCATC TCGTAAGGCG GGTTTGGCGT TATCGCCTCC CTTAACACGG 
GCCATTTCGC AGCCGGTGGG AGTTATCCCG AAGCTCCAAT CCGCTATCCC GAAGGCTAAG 
GAGCATAAGA TGCCAGAGCC GGGTGATCTC GCACGGGCAA CAGGGGACGG CTCGTCTGCC 
AAGGGAGATA AGAAGTTCCT TGGGATGAAG ATCCGTCCTT CTGCCATCCC ACGCTATCGG 
CATAGGAATT CGAAGGTGTT CACTCTTGCA CGTCACTTCG AGGAGTTGAG TCGAGAGTTT 
GAGAAGGAGC GAATCAAGGA CAAGAGAGAG CGGCAGGCCA AGATGTCTTT TCCTCGTGCA 
TATCTGCCAC GTTCAACAAC CAAGGCTATC GTGGAGATCT ACGAGGATGT CGACCAGGCC 
GTTCAAGAAC CCGGTACCGC GGAAGAGGGC CATCCTGGTG ACAGAGACTC GAAAGACAGA 
AGACCAATAG AGGGCTCTTC TCCGTCGGAT CAAATCCAAG GGGATCCTGC CAGATTGTCT 
GCAGACCATG GTCGCACTTC GCCGATTGAG ATCCCATCTC AAGAGCATAC CCATCAAGAT 
GGGAACCATG AAGCGGAGGG AGACGAAGGA CAGCACGAGA GCGAGGTTGA CTCCGATGGT 
GAAGGTGCAG ATAGTGATGC CGACCGGTCA TCATATACGT ATGACGATAT CCCGACCGAT 
GTGAGGGACC TTGCTGCATC CATCGAGCCA AGTGAGGAAA TACCTGAAGA GCTGCCGAAG 
CATCAAAGGA AGAGTCTCAT GACAATGCTC ACCAACTTCT GGGCCGAACG GTCTGCCAGT 
GGATGGCCTA CGCTTGACTA CCCTTACAAT TCAACCGACC ACATGTTTAC GGACTCGAAC 
GTGATTGTCA GAGAAGACGA GCCAAGTTCG GTTGTGGCCT TTGCGATGAA CTCCACGGAC 
TACCATATCC AACTTCGAAA CATTCGAAGG AACTCTAGGA GAACCCAGCA GCAGCAACAA 
TCACAAAGGC AGCAGAAAGA GCAACAGCAA GAGCGAGCCT CCCAGGATTT CGAAGAGAGT 
AGTGACAGCG GCATGCTAGA GATTAAGAGC GGCGGCGCCG GTCCAGACCT CAGCGAAGCC 
GAACTAGAAC AGAGCATGCT CCGCCCCTTT GGCACCCACC TCAAGTTCCA ATTCATGGAA 
GGCTCCGGCA AGTTCACTTG CAAGATCTTC TTCGCCGAGC AATTCGACGC GTTGCGACGC 
AAATGCGGCG TCGGCGACCG TATTGTCGAA TCGCTCTCTC GTTGTCTCAA ATGGGACTCC 
AAAGGCGGCA AGACCAAATC GGTCTTCCTC AAGACTCTCG ATGACCGACT CGTCATGAAA 
TCGCTTTCTC CCGTCGAGAC CTCCGCCTTT CTCGGTTTTG CGCCCGTCTA CTTTGAGTTT 
ATGGCCCAAG CCCTGTTTCA TGAACTCCCC TCCGTCATCG CCAAGATGTT GGGTTTCTTC 
CAAGTGATCA TCAAAAACCC CATCACCAAC ACTGAAGTCA AACTCGACCT GCTGCTCATG 
GAGAATCTGT TCTACGACCG CTCACCAACC CGCACGTTCG ACCTCAAGGG GTCCATGCGC 
AATAGAAAGA TCCAATCAAC CGGCGAACAA AACGAAGTTC TGTTGGACGA AAACATGGTC 
GAGTACATCT GGGAATCCCC TCTCTTCGCG CGGGAGCACT CCAAACGGCT CCTGCGCGCA 
TCGGTATGGA ACGATACGCT TTTCCTCGCG CGCCAAAACG TGATGGATTA CTCGTTGATG 
ATCGCCGTCG AGGAGAACAA GAAGGAGCTG GTGGTGGGCA TGATTGATTG CATCCGCACC 
TGGACGTGGG ACAAACGCCT CGAAAGCTGG ATCAAAGATA GGGGGTTTGC GGGAGGAGGG 
AGGAATCGGC CGACGGTGAC GAGTCCGAAG GAGTATAAGA GTCGTTTTCG CGAGGCTATG 
GCTAGGTATA TCCTCCAAGC ACCTAATTGC TGGCATCACT TTGGAAGTGG CCTAGGCTCG 
ACCGGTAATC CTTCTTTGCA GGCGGTTGGA CATGCTCATA GGGCGAGGTT CGCGGGTGAT 
GTTACCCCGT CATCAGGGAG GGGGTCGGTG AGAGGGTCGG TGAGGGGGAT GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_964610.1
CDS	959..8635
Translation	MASSSHRPFKLPLGPLSRSNNGDHSSESDYPGGRRDSMAALSISSHVNQEQLAHTLDRIHNTASQSGVLTTFNDFAPPPPPTVPVAEKKGTTGDLVQQGLSGLYSRLKEVVGVAGKPSAQEAESSTNTQDATPKNFKSPIPNSPNNSSTKISVASPPKGDGSSIKSPFDTKSPFDTKSPFDAKSPFEARHAFDGPASLDIPPAVSTGTSPISPTTIPADLQSPPQTTRPPSLGTLPSLKTGNSSAQTIPRTGRTSQSTAASPTSAPFMGKASLERDSSRGPARHLDEPSSWGSSRRSISRHGDGSTSPSIAVRPSVDTTTAALARSRMEDAVSIDGGIGSPMSPSGPIPDVCISSASSRLSEATQVKRRPAIIDRISQSRVHSHSRSSSMEPGKAQPSPISTSAHSTIYHDSFTHNVKPQRLQSGAMKIPGTTTNEGAPEVVNARLEMMRKQVLSKEFWMKDETTKECFACGSPFTAFRRKHHCRTCGCIFCSKCTSTISGQKFGVQGTLRVCKNCLEVINRRYESGSDDSADEPFLPAIFRSNQTKPPPLALARPQADDEVSIAERTEQANDTRSAKTPMMAIPATRRVGDSNRHSAVLEIDMPQLSRPGSSRSLRSLAAGRPQSSSHRRLHSKHTFLTRFKSSATDDRAPFRKPASEDLGRKSKLSAFHDDNIIDPELAPYMSDESTDDEQMSSIFATMNSNDAPAASLDPDKTSMGAFMNAANRKHRFRHGEKSISGLSYTSRGHEDTGLPTLSLHTRPTRKRNLSTASVTGHPLRSPRPRSGIFSMMLNPGASADALSLWDNPGVVEASALSRRDSYFEGRSSTGGLNPISMNHVKKLFRQLLNDAEIPNPAAWEKALIPILDRCADDVDPDVRNGDDMDIRHWIKLKKIPGGKPGDTAYVHGVVFSKNLALKTMPRKIENPKIVIITFPIEYQRHQEQHFMSLQPVIEQEKEYLRMVVNRILNLEPRVLLVEKNVSGVALQYLSEANVAVVYNVKPSVIEAVSRIANIPIISSMDMLSLGARVGTCQSFEVKTFVNKELKGRKKTYIFISGCPKERGCTIALRGGSTEILSRMKRITEFMVYVFYNLKLESCLMRDEFVYVPEELESPPRSTTKLLSNGSQDPSSTTPSAQTGENSPHSVIHHISEHDQSLEPSQAAARPVGSTTSAEEYSAAALDTSTSIKEETEEELLRAESDTHEGQGAEDLPMPTYYGDMVAKYETRILSASPFVKFTQPYLLMKAREQERRLVYLKRLRDQDIVEELGESEKSEPQPRQFQLIKPEMVHELGQKAPRHIMEVLHAVHDAEYDKALYNYQTQTRQWENYIQGSLDLFDPYSHQSIVILYSVTCNVTQVPCTEPSVVAFEFYKENPDPLSGMYQDCTLGQYIEDVCESADSICHSNGCDRKMFEHYQTYVHENARITVMVEDKPKWPENFPEKPHERDGWQDGTGICMWNYCKLCNKHFGLMPMSVSTWKYSFAKYLELSFWSRGLRLHPETGCPHDHKKDHVRFFYYLYRDIAVRIHYDPIDLYEIIVPRSRITWKVDHDLELKNQVFLKAEERWNRFINSVKARLKSIRIDSVLPEKTEHCKAEVERMTKKAQEDQAELIQDLQDAYMGSKYYEVLPMNGVIRQMAERATEWDVAFTKFEADFLSDKDVRQLTIIQLKKMFDPKSGSSGAELGEKPEVTHSNVNAGEIEVKEKPSQPTEDEGPSPKMHSPVSSATKPIPVVREEAVEEAGSILDGVEHLDLATASSPTLLRKGDPVLEAAKVTVNEPTPIVAPAAPTQQPTSLSKVDEAPHESSLAEMIEPKRREQHVAMPEATTHAAGGEGSMEGYKPVVPERTSSRKAGLALSPPLTRAISQPVGVIPKLQSAIPKAKEHKMPEPGDLARATGDGSSAKGDKKFLGMKIRPSAIPRYRHRNSKVFTLARHFEELSREFEKERIKDKRERQAKMSFPRAYLPRSTTKAIVEIYEDVDQAVQEPGTAEEGHPGDRDSKDRRPIEGSSPSDQIQGDPARLSADHGRTSPIEIPSQEHTHQDGNHEAEGDEGQHESEVDSDGEGADSDADRSSYTYDDIPTDVRDLAASIEPSEEIPEELPKHQRKSLMTMLTNFWAERSASGWPTLDYPYNSTDHMFTDSNVIVREDEPSSVVAFAMNSTDYHIQLRNIRRNSRRTQQQQQSQRQQKEQQQERASQDFEESSDSGMLEIKSGGAGPDLSEAELEQSMLRPFGTHLKFQFMEGSGKFTCKIFFAEQFDALRRKCGVGDRIVESLSRCLKWDSKGGKTKSVFLKTLDDRLVMKSLSPVETSAFLGFAPVYFEFMAQALFHELPSVIAKMLGFFQVIIKNPITNTEVKLDLLLMENLFYDRSPTRTFDLKGSMRNRKIQSTGEQNEVLLDENMVEYIWESPLFAREHSKRLLRASVWNDTLFLARQNVMDYSLMIAVEENKKELVVGMIDCIRTWTWDKRLESWIKDRGFAGGGRNRPTVTSPKEYKSRFREAMARYILQAPNCWHHFGSGLGSTGNPSLQAVGHAHRARFAGDVTPSSGRGSVRGSVRGME

Target ORF information:

RefSeq Version	XM_959517.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A 1-phosphatidylinositol-3-phosphate 5-kinase (NCU02083), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_959517.2

ORF Insert Sequence:

ATGGCTTCCA GCTCACACAG GCCATTCAAG CTCCCCCTTG GTCCCCTTTC GCGCTCCAAC 
AATGGCGACC ATTCCTCCGA GTCCGACTAT CCGGGCGGCC GTCGCGACTC CATGGCCGCC 
CTCTCCATCT CTTCTCATGT CAACCAGGAA CAGCTCGCTC ACACCCTTGA CCGAATTCAC 
AATACAGCAA GCCAATCTGG GGTCCTGACC ACCTTCAATG ATTTCGCCCC GCCACCTCCA 
CCTACTGTGC CTGTTGCTGA GAAAAAGGGA ACTACGGGTG ACTTGGTACA GCAAGGCTTG 
AGTGGTCTCT ACAGCCGTTT AAAGGAGGTT GTTGGGGTGG CAGGGAAACC TTCTGCCCAG 
GAGGCTGAAA GTTCGACTAA CACCCAGGAT GCCACGCCCA AGAATTTCAA GAGCCCGATT 
CCCAATAGCC CCAACAACAG TAGCACCAAG ATCTCGGTTG CCTCCCCTCC AAAGGGAGAC 
GGTTCGTCCA TCAAGTCACC TTTTGATACA AAGTCGCCTT TTGATACAAA GTCGCCCTTC 
GATGCAAAAT CACCCTTTGA AGCAAGGCAT GCCTTTGATG GGCCCGCCTC TCTTGACATC 
CCACCCGCTG TGTCTACCGG CACATCTCCA ATTTCGCCAA CCACCATACC AGCAGACCTC 
CAGTCCCCCC CACAGACAAC CAGGCCACCA TCACTTGGTA CGCTGCCGAG CCTCAAGACC 
GGAAACTCAA GCGCACAGAC AATTCCGAGG ACGGGAAGGA CGTCCCAGTC AACCGCGGCC 
AGCCCTACCT CTGCCCCCTT CATGGGAAAA GCGTCTCTCG AAAGAGACAG CTCTAGGGGT 
CCTGCCAGAC ACCTCGATGA GCCCTCAAGT TGGGGTTCTA GCCGGCGGAG CATCAGCAGA 
CATGGTGACG GATCCACGTC GCCTAGCATT GCCGTGAGAC CAAGTGTAGA TACCACGACG 
GCCGCTTTGG CGCGCAGTAG AATGGAGGAC GCTGTGAGTA TAGATGGAGG CATCGGCAGT 
CCCATGAGCC CTTCTGGGCC TATACCAGAC GTGTGCATCT CCTCTGCCTC CTCGCGCTTG 
TCTGAGGCCA CTCAAGTCAA GCGAAGGCCT GCCATTATTG ACCGCATAAG CCAGTCCAGA 
GTTCATAGCC ATTCCAGGTC CTCGTCCATG GAGCCTGGCA AGGCACAGCC CAGTCCCATC 
AGCACATCGG CTCATAGCAC TATCTATCAT GATTCGTTTA CTCACAATGT CAAACCACAG 
AGGTTGCAAT CTGGAGCAAT GAAAATCCCA GGCACCACGA CAAACGAAGG AGCCCCTGAG 
GTCGTCAATG CCAGACTAGA AATGATGAGG AAGCAGGTCC TTAGCAAAGA GTTCTGGATG 
AAAGACGAAA CCACCAAAGA ATGCTTTGCC TGCGGGAGCC CGTTCACTGC CTTTCGGCGC 
AAGCACCACT GCCGCACGTG TGGCTGCATC TTCTGTTCAA AATGCACCTC TACCATTTCA 
GGACAAAAGT TTGGCGTCCA GGGAACGCTT CGCGTCTGCA AGAATTGCTT GGAGGTCATA 
AACCGCCGCT ATGAAAGCGG CTCAGATGAT TCGGCAGATG AGCCATTCTT GCCCGCCATC 
TTTCGTTCCA ATCAGACCAA GCCGCCTCCT CTTGCTCTTG CTCGACCGCA GGCGGATGAT 
GAGGTTAGTA TCGCGGAGCG AACGGAGCAA GCAAATGACA CAAGGTCCGC CAAGACACCG 
ATGATGGCGA TTCCGGCCAC ACGCCGAGTT GGCGACAGCA ATCGCCACTC GGCCGTTTTG 
GAGATTGATA TGCCACAGCT CAGCCGCCCC GGATCATCTC GGTCGCTGAG ATCACTCGCG 
GCGGGCCGAC CGCAATCATC TAGTCATCGC AGGCTTCATT CAAAACATAC CTTCTTGACT 
AGGTTCAAGT CTTCTGCGAC TGACGATAGG GCACCGTTTC GCAAGCCTGC TAGCGAAGAC 
CTTGGGAGGA AATCCAAGCT CAGCGCCTTC CATGACGACA ACATCATTGA TCCAGAACTT 
GCCCCTTACA TGTCGGACGA GTCAACTGAT GACGAACAAA TGAGTAGCAT ATTTGCAACC 
ATGAACAGCA ATGATGCGCC AGCGGCCAGC CTCGATCCCG ACAAAACAAG CATGGGTGCC 
TTTATGAATG CTGCAAACAG AAAACATCGG TTCCGTCACG GCGAGAAGAG CATCAGTGGA 
CTTAGTTATA CCAGCAGAGG CCACGAAGAC ACTGGCTTAC CGACTCTCTC TCTCCACACC 
CGGCCAACCA GAAAGAGAAA TCTCAGCACT GCAAGTGTCA CGGGCCATCC GCTGCGGTCA 
CCCCGCCCAA GATCTGGCAT CTTTAGCATG ATGCTAAACC CTGGTGCTTC AGCTGACGCC 
TTGTCTCTCT GGGATAATCC TGGAGTTGTC GAAGCATCTG CACTCAGTAG AAGGGATTCT 
TACTTTGAGG GCAGGTCATC AACCGGTGGA TTAAACCCCA TAAGCATGAA CCACGTCAAG 
AAGCTTTTCC GGCAGCTTTT GAACGATGCA GAAATCCCCA ACCCTGCAGC GTGGGAAAAG 
GCCCTCATCC CAATCCTTGA TAGATGTGCT GATGACGTTG ATCCGGATGT CCGTAACGGA 
GATGACATGG ACATTCGCCA CTGGATCAAG TTGAAAAAGA TACCTGGCGG CAAACCTGGC 
GATACTGCTT ACGTCCATGG AGTCGTCTTT TCCAAGAACT TGGCCCTCAA GACCATGCCC 
CGCAAGATCG AGAATCCCAA AATCGTCATC ATCACTTTTC CAATCGAGTA TCAGCGGCAC 
CAGGAGCAGC ATTTTATGAG CTTGCAGCCG GTCATAGAGC AGGAAAAGGA GTATCTGAGG 
ATGGTGGTGA ACCGAATCCT GAACCTCGAG CCTCGGGTGT TGCTCGTCGA AAAGAACGTC 
TCTGGCGTGG CTTTACAATA TCTCTCGGAA GCCAACGTCG CGGTCGTGTA TAACGTCAAA 
CCGTCAGTTA TCGAGGCTGT TTCCAGAATT GCCAATATTC CCATCATCTC ATCCATGGAT 
ATGCTTAGCT TGGGTGCGCG CGTCGGGACC TGCCAAAGTT TTGAAGTCAA GACCTTCGTC 
AACAAGGAGC TCAAAGGCAG GAAGAAGACG TACATTTTTA TCTCTGGCTG CCCAAAAGAG 
CGCGGCTGTA CTATTGCTCT TCGCGGCGGG TCGACAGAGA TTCTGTCCCG CATGAAGAGA 
ATCACCGAGT TCATGGTTTA TGTGTTTTAC AACCTAAAGC TCGAATCCTG TCTTATGCGC 
GATGAATTTG TTTATGTGCC TGAAGAGCTC GAGTCCCCTC CACGATCAAC TACGAAGCTC 
CTGAGTAACG GCAGTCAGGA CCCGTCGTCA ACTACTCCAA GCGCGCAGAC TGGGGAAAAT 
TCCCCTCACA GCGTGATCCA CCACATTTCC GAACATGATC AGTCTTTGGA ACCATCTCAA 
GCGGCTGCTA GACCTGTCGG GAGCACAACT TCGGCCGAGG AATATTCAGC TGCCGCCCTC 
GACACCAGCA CGTCGATCAA GGAAGAGACT GAGGAGGAAT TGCTTCGGGC TGAGTCAGAC 
ACCCATGAAG GTCAAGGTGC GGAAGACTTG CCTATGCCGA CATATTATGG TGACATGGTA 
GCCAAATACG AGACCAGAAT TCTCTCTGCG TCACCCTTCG TCAAGTTCAC TCAACCTTAC 
CTGCTCATGA AAGCCCGAGA GCAGGAGAGG CGCCTGGTGT ATCTCAAACG TCTTCGTGAC 
CAGGACATCG TCGAAGAACT CGGAGAGAGC GAGAAGAGTG AGCCTCAGCC TAGACAATTT 
CAGCTTATCA AGCCTGAAAT GGTGCATGAA CTTGGCCAAA AGGCACCGCG CCACATCATG 
GAGGTGCTCC ATGCTGTCCA CGACGCGGAA TACGACAAGG CCTTGTACAA CTATCAGACT 
CAAACCAGAC AGTGGGAGAA TTATATCCAG GGAAGCCTCG ACCTGTTTGA TCCATACTCC 
CATCAGAGCA TCGTTATTCT GTACTCTGTC ACATGCAACG TGACCCAAGT TCCATGCACG 
GAGCCCAGCG TGGTGGCCTT TGAGTTCTAC AAGGAGAATC CAGACCCACT CAGTGGAATG 
TACCAAGATT GCACCCTGGG GCAGTACATA GAAGACGTCT GCGAAAGCGC CGACTCAATC 
TGTCACTCCA ATGGATGCGA CCGAAAGATG TTTGAGCATT ACCAGACATA TGTTCACGAA 
AACGCGCGAA TTACTGTCAT GGTGGAGGAC AAGCCCAAAT GGCCAGAGAA CTTCCCCGAG 
AAACCGCACG AAAGGGATGG TTGGCAAGAT GGCACCGGCA TCTGCATGTG GAATTACTGC 
AAGTTATGCA ACAAACATTT CGGACTGATG CCCATGTCAG TGAGCACCTG GAAGTATTCT 
TTCGCAAAGT ATCTGGAACT GTCGTTCTGG AGCCGTGGTT TACGCCTTCA CCCAGAGACA 
GGATGTCCTC ACGATCATAA AAAAGACCAT GTTCGCTTCT TTTATTACCT ATACCGCGAC 
ATTGCCGTCA GGATACACTA CGACCCGATC GACTTGTACG AAATCATTGT ACCTAGATCT 
CGCATCACCT GGAAGGTTGA CCATGATCTG GAGTTGAAAA ATCAAGTCTT TCTCAAGGCC 
GAGGAGCGCT GGAACCGCTT CATCAACTCC GTAAAAGCCA GGCTGAAGAG CATTAGAATC 
GATAGCGTCT TGCCGGAGAA AACGGAGCAT TGCAAGGCTG AGGTGGAAAG AATGACAAAG 
AAGGCACAAG AGGACCAGGC CGAGCTGATT CAGGACCTTC AGGATGCCTA CATGGGCTCA 
AAGTACTACG AGGTTCTTCC CATGAACGGA GTCATACGTC AAATGGCTGA GAGAGCTACT 
GAATGGGACG TTGCCTTCAC TAAATTTGAG GCCGACTTTC TTTCGGACAA AGATGTTCGC 
CAGTTGACTA TCATCCAGCT GAAGAAGATG TTTGACCCAA AATCTGGATC CTCAGGTGCG 
GAACTTGGGG AGAAGCCTGA GGTAACTCAC TCCAATGTCA ACGCCGGCGA AATTGAGGTG 
AAAGAAAAGC CATCGCAGCC AACGGAAGAT GAAGGCCCCA GTCCCAAGAT GCATTCTCCA 
GTGTCTTCAG CGACCAAACC TATTCCTGTG GTGAGGGAGG AAGCAGTAGA AGAGGCCGGG 
TCCATCCTGG ATGGAGTTGA GCATCTTGAC CTTGCAACTG CATCCTCGCC CACGCTCCTC 
AGGAAGGGGG ATCCCGTCCT GGAAGCAGCA AAGGTCACTG TCAATGAGCC AACGCCGATT 
GTTGCACCAG CAGCCCCTAC ACAACAACCA ACATCTCTCT CAAAAGTTGA CGAAGCGCCT 
CATGAGTCGT CTCTAGCAGA GATGATCGAA CCCAAACGCC GAGAACAACA TGTAGCCATG 
CCTGAGGCTA CCACCCATGC TGCCGGCGGC GAGGGTTCGA TGGAAGGTTA CAAGCCCGTT 
GTACCGGAGC GCACGTCATC TCGTAAGGCG GGTTTGGCGT TATCGCCTCC CTTAACACGG 
GCCATTTCGC AGCCGGTGGG AGTTATCCCG AAGCTCCAAT CCGCTATCCC GAAGGCTAAG 
GAGCATAAGA TGCCAGAGCC GGGTGATCTC GCACGGGCAA CAGGGGACGG CTCGTCTGCC 
AAGGGAGATA AGAAGTTCCT TGGGATGAAG ATCCGTCCTT CTGCCATCCC ACGCTATCGG 
CATAGGAATT CGAAGGTGTT CACTCTTGCA CGTCACTTCG AGGAGTTGAG TCGAGAGTTT 
GAGAAGGAGC GAATCAAGGA CAAGAGAGAG CGGCAGGCCA AGATGTCTTT TCCTCGTGCA 
TATCTGCCAC GTTCAACAAC CAAGGCTATC GTGGAGATCT ACGAGGATGT CGACCAGGCC 
GTTCAAGAAC CCGGTACCGC GGAAGAGGGC CATCCTGGTG ACAGAGACTC GAAAGACAGA 
AGACCAATAG AGGGCTCTTC TCCGTCGGAT CAAATCCAAG GGGATCCTGC CAGATTGTCT 
GCAGACCATG GTCGCACTTC GCCGATTGAG ATCCCATCTC AAGAGCATAC CCATCAAGAT 
GGGAACCATG AAGCGGAGGG AGACGAAGGA CAGCACGAGA GCGAGGTTGA CTCCGATGGT 
GAAGGTGCAG ATAGTGATGC CGACCGGTCA TCATATACGT ATGACGATAT CCCGACCGAT 
GTGAGGGACC TTGCTGCATC CATCGAGCCA AGTGAGGAAA TACCTGAAGA GCTGCCGAAG 
CATCAAAGGA AGAGTCTCAT GACAATGCTC ACCAACTTCT GGGCCGAACG GTCTGCCAGT 
GGATGGCCTA CGCTTGACTA CCCTTACAAT TCAACCGACC ACATGTTTAC GGACTCGAAC 
GTGATTGTCA GAGAAGACGA GCCAAGTTCG GTTGTGGCCT TTGCGATGAA CTCCACGGAC 
TACCATATCC AACTTCGAAA CATTCGAAGG AACTCTAGGA GAACCCAGCA GCAGCAACAA 
TCACAAAGGC AGCAGAAAGA GCAACAGCAA GAGCGAGCCT CCCAGGATTT CGAAGAGAGT 
AGTGACAGCG GCATGCTAGA GATTAAGAGC GGCGGCGCCG GTCCAGACCT CAGCGAAGCC 
GAACTAGAAC AGAGCATGCT CCGCCCCTTT GGCACCCACC TCAAGTTCCA ATTCATGGAA 
GGCTCCGGCA AGTTCACTTG CAAGATCTTC TTCGCCGAGC AATTCGACGC GTTGCGACGC 
AAATGCGGCG TCGGCGACCG TATTGTCGAA TCGCTCTCTC GTTGTCTCAA ATGGGACTCC 
AAAGGCGGCA AGACCAAATC GGTCTTCCTC AAGACTCTCG ATGACCGACT CGTCATGAAA 
TCGCTTTCTC CCGTCGAGAC CTCCGCCTTT CTCGGTTTTG CGCCCGTCTA CTTTGAGTTT 
ATGGCCCAAG CCCTGTTTCA TGAACTCCCC TCCGTCATCG CCAAGATGTT GGGTTTCTTC 
CAAGTGATCA TCAAAAACCC CATCACCAAC ACTGAAGTCA AACTCGACCT GCTGCTCATG 
GAGAATCTGT TCTACGACCG CTCACCAACC CGCACGTTCG ACCTCAAGGG GTCCATGCGC 
AATAGAAAGA TCCAATCAAC CGGCGAACAA AACGAAGTTC TGTTGGACGA AAACATGGTC 
GAGTACATCT GGGAATCCCC TCTCTTCGCG CGGGAGCACT CCAAACGGCT CCTGCGCGCA 
TCGGTATGGA ACGATACGCT TTTCCTCGCG CGCCAAAACG TGATGGATTA CTCGTTGATG 
ATCGCCGTCG AGGAGAACAA GAAGGAGCTG GTGGTGGGCA TGATTGATTG CATCCGCACC 
TGGACGTGGG ACAAACGCCT CGAAAGCTGG ATCAAAGATA GGGGGTTTGC GGGAGGAGGG 
AGGAATCGGC CGACGGTGAC GAGTCCGAAG GAGTATAAGA GTCGTTTTCG CGAGGCTATG 
GCTAGGTATA TCCTCCAAGC ACCTAATTGC TGGCATCACT TTGGAAGTGG CCTAGGCTCG 
ACCGGTAATC CTTCTTTGCA GGCGGTTGGA CATGCTCATA GGGCGAGGTT CGCGGGTGAT 
GTTACCCCGT CATCAGGGAG GGGGTCGGTG AGAGGGTCGG TGAGGGGGAT GGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU02083 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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